FvH4_3g44570

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37128701 .. 37133579
4879 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44570.t1

Sequence Viewer

Length: 3279 bp
ATGAAAATCAGGATTGAGTTGCCTGCGTTCTTGTTAAGGGGTCCTGACACTCGCAAAGGGATTACAGCCCAAATACGCGATCGACTTCGTAGAAGTGGCATTATAACGTTCATGGATGAACCAGATCTTAGAGTAGGGGATACCATTTCGCCCACTCTCATAAAGGCAATTAAAGAGTCGAGGTTTGCAATCGTAATTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTGTCCATGGAAGACAACAGAATTTTGCCACTTTTTTTTGAAGTTGATCCTACTGATGTTCGACATCAGAAGAAGAGTTTCGAAGAAGCTTTCTCTAAGCATGAAACCTCTGGCCGACATGAATCAGACAAGTTGAATCAATGGAGGGCTGCCATAAGCAAAGTGGCCAATATCTCAGGGTGGAACACAAATGATTATAAGACTCACGAAGAACTTGTTGACAAGATTGTGGAATCTCTCCGCAGTGAAGTACCACCAGATGCAATTGAATTCACAGATGATTTCCAAGCATTTGGAGCAACAAAAGAAGCGACAGACAAGGTTATGAAGGCGCTAACAGATGACAAGATTACTGCCGTTGGTGTCTATGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAGAAATGGTACTTTTAACCACTGGATTATGGCTGTCGTATCCCAAAACCTTGACTTGGAAAAAGTTCAAGGTACATTGGCAGATTTGTTGGGCTTTAAATTCGTCGAGGAGACAGAAGCTGGAAGAGCAAATAAGTTGCATAAGGAGATCATGAGAAAAGAAAAACTCCTGATAATTGTGGACGATGTTTGGGAGAGAACAGAGTTGTCAAAGATAGGGATTCCCACCTACAATGAGCTTCAAAAGTGCAAGTCCAAAGTGCTACTCACCACCAGGAGATTGAATGTCTGTAATGCCATGAAGTGCCATGAAAAGATCAGCCTCAAAGTTCTCTCAGATCAAGATTCTTGGACCTTGTTCGTGAGAAATGCAGGAACAATTTCTTTTGAGCCCACCTCATTTGAGAAAGTGGCAAGGAGGGTAGCTTTAGAGTGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCAGGAGGAATGGGAGAAGGCAGCTAACAGCCTAGAGCAGTCGCAATATGTCAATCCCAACCATGAGGAAGATGAGGAAAATGCGTTCAGTTGTATACGATTAAGCTATGATTACTTGAAAAATGACGACCACAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCATGCCATCTACATAGAAGACTTGTTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGCGAAAACAATGGAAGTAGCCAGAGGAAGATTAGTTACAGTGGTCAAGTACCTGAAAAATTCTAGCTTGCTTTTGGATAGTGAGGAAAAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCCAAATCTGAAGATTGCCATCGGTTTTTGGTGGAAGCTGGTCGTGGTTTAAAGGATTGGGCTCCACGGGTATTGCATGAAGGCTGCACTGCAATTTCACTGATGAACAACAAAATTCGCAAGCTACCTGAAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAACGGGAATGCTGTACTAAGTGAGATCCCCGAAAAGTTCATCCTGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGTTACACTGATATTTCTGTATTACCCCAATCATTTAGTCTCCTCACCAACCTCCAAGCTTTGTATTTAAATGGTTGCGACAAATTGATTGACATTTCTGTTGTGGGGAAACTTAAGAAGCTTGAAATTCTTAGTATCAGAGACTGTTCCATGGGGAAATTGTCAAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGACGTCAATTCCTCTGCGATACGATTCATAATTAGAAGAGGACGGCTGATGTGCACATTTCCCTTTACAATTCCATCTAAAGTGATACCAAAGTTGCATGAATTAGAAGAATTATACATGGTGTACTGTGGCTTTGAGGAGAACGGGTGGTGTGTAGCTGAGGGACCAACAGAAACAACCAATATTATTAGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCTTATCCGATGAAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACTTTTGTATAACTATCAACGGCAGATACCCCAACTCTGTATACGAACAAGGAGATCATAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTACAACCGTTGCGGAGGGATGAGTGACATTGTTATGGAATATGACCATGGCAGGTTACATAAACTCAAGCATCTCACAGTTGCTGGGTGTGCTCGTGATTCTTCTTATGTGTACTTGAAAGAGTTGGTGAACACAACAAGACGAGTTGAAACCGGACCCGTGTTTGAGAATTTGGAAGAGTTGCATCTGAAATTTCTGAAGCACCTGGAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTGTTTCATGTGTTTTCGTGTACGATTTTGAGGAGTGTATCAGAATTTGTACAGAGACTACCAAATCTGGAGAAACTAGATTTAAACTATATGGTCAAATTGGAATATGTGTTTAGATACGAAGGGTTCGAGCCAGAACAATCAAAACTGAGGGAGATGCATTTGTTGCGTCTAGAAGCACTAAGAAGCATATGTAATGGTCCCGCTCCACATGCAATGTTCCAGAGTCTTAAAATTTTGACCATTGACCGTTGCCTGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCGGACTGTCTTGTTCAATTGGAAGAACTTCTTGTAGTGGATTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGACCAAGGCGAAGACGGTTCTACCAAAACTGAAGAACTTGGGTTTGATGAATCTTCCTATGTTGTATGGTCCAATTGCTGCCGTTGATATTGAGTGTCCTTCATTGGAACACTTGATGGTGGAGGATTGCCCCAAGCTTTTATTTTCAACTTTTTCCGATCTCTTCAAAGCCTTCAAAAGCAGGAACCAATTTTCATTTTCAGCCTCGGCGTGTCAGAACATCTAG

Protein Analysis

1093

Amino Acids

124.12

Weight (kDa)

5.95

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 165 1.9e-38 TIR domain
TIR_2 PF13676 21 - 102 1.9e-13 TIR domain
NB-ARC PF00931 188 - 351 4.8e-30 NB-ARC domain
LRR_8 PF13855 563 - 622 5.7e-10 Leucine rich repeat
LRR_14 PF23598 567 - 666 3e-14 Leucine-rich repeat region
LRR_14 PF23598 616 - 744 1.6e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 872 - 1008 4.7e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 104, 453
AatII GACGTC 1 cut(s) 1992
Acc36I ACCTGC 2 cut(s) 2209, 2475
AccBSI CCGCTC 1 cut(s) 2897
AccI GTMKAC 3 cut(s) 1123, 1264, 2322
AccII CGCG 2 cut(s) 78, 1376
AciI CCGC 6 cut(s) 496, 633, 2405, 2445, 2895, 2981
AclI AACGTT 1 cut(s) 107
AclWI GGATC 4 cut(s) 296, 1729, 1788, 2343
AcoI YGGCCR 2 cut(s) 367, 420
AcuI CTGAAG 4 cut(s) 1569, 1689, 2651, 3104
AcyI GRCGYC 1 cut(s) 1989
AfiI CCNNNNNNNGG 3 cut(s) 736, 1977, 3031
AflII CTTAAG 1 cut(s) 1898
AflIII ACRYGT 1 cut(s) 661
AhdI GACNNNNNGTC 1 cut(s) 1303
AjnI CCWGG 4 cut(s) 953, 1170, 2637, 2668
AloI GAACNNNNNNTCC 2 cut(s) 1238, 1270
Alw21I GWGCWC 2 cut(s) 2042, 2527
Alw26I GTCTC 6 cut(s) 652, 785, 1829, 1920, 2679, 2740
Alw44I GTGCAC 1 cut(s) 2038
AlwI GGATC 4 cut(s) 296, 1729, 1788, 2343
AlwNI CAGNNNCTG 3 cut(s) 800, 1929, 2516
AoxI GGCC 2 cut(s) 367, 420
ApaLI GTGCAC 1 cut(s) 2038
ApeKI GCWGC 7 cut(s) 404, 668, 1190, 1623, 2950, 2953, 3131
Asp700I GAANNNNTTC 4 cut(s) 332, 744, 1060, 3006
AspLEI GCGC 1 cut(s) 589
AspS9I GGNCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
AsuC2I CCSGG 1 cut(s) 1520
AsuHPI GGTGA 6 cut(s) 667, 940, 1822, 2419, 2572, 2669
AsuII TTCGAA 1 cut(s) 336
AvaII GGWCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
BaeGI GKGCMC 2 cut(s) 1156, 2042
BaeI ACNNNNGTAYC 2 cut(s) 2063, 2096
BalI TGGCCA 1 cut(s) 422
BanII GRGCYC 2 cut(s) 1074, 1603
BarI GAAGNNNNNNTAC 4 cut(s) 1420, 1452, 2417, 2449
BauI CACGAG 1 cut(s) 2526
BbsI GAAGAC 4 cut(s) 273, 1344, 1365, 3071
Bbv12I GWGCWC 2 cut(s) 2042, 2527
BbvCI CCTCAGC 1 cut(s) 2145
BbvI GCAGC 7 cut(s) 391, 655, 1202, 1610, 2937, 2940, 3118
BccI CCATC 5 cut(s) 1355, 1566, 2068, 2381, 3163
BceAI ACGGC 4 cut(s) 596, 2045, 2317, 3119
BciT130I CCWGG 4 cut(s) 955, 1172, 2639, 2670
BciVI GTATCC 3 cut(s) 133, 730, 1516
BcnI CCSGG 1 cut(s) 1520
BcoDI GTCTC 6 cut(s) 652, 785, 1829, 1920, 2679, 2740
BfaI CTAG 6 cut(s) 1202, 1464, 2429, 2768, 2864, 3277
BfmI CTRYAG 1 cut(s) 679
BfoI RGCGCY 1 cut(s) 590
BfrI CTTAAG 1 cut(s) 1898
BfuAI ACCTGC 2 cut(s) 2209, 2475
BfuI GTATCC 3 cut(s) 133, 730, 1516
BglII AGATCT 1 cut(s) 124
BisI GCNGC 8 cut(s) 405, 634, 669, 1191, 1624, 2951, 2954, 3132
BlsI GCNGC 8 cut(s) 406, 635, 670, 1192, 1625, 2952, 2955, 3133
Bme1390I CCNGG 5 cut(s) 955, 1172, 1520, 2639, 2670
Bme18I GGWCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
BmeRI GACNNNNNGTC 1 cut(s) 1303
BmgT120I GGNCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
BmiI GGNNCC 6 cut(s) 42, 1602, 2151, 2590, 2893, 3239
BmrFI CCNGG 5 cut(s) 955, 1172, 1520, 2639, 2670
BmsI GCATC 7 cut(s) 505, 1390, 1494, 2512, 2626, 2838, 2978
BpiI GAAGAC 4 cut(s) 273, 1344, 1365, 3071
BplI GAGNNNNNCTC 2 cut(s) 1061, 1093
BpmI CTGGAG 3 cut(s) 1679, 2660, 2780
Bpu10I CCTNAGC 1 cut(s) 2145
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 2483, 2672
BpuMI CCSGG 1 cut(s) 1520
BsaBI GATNNNNATC 1 cut(s) 1557
BsaHI GRCGYC 1 cut(s) 1989
BsaI GGTCTC 1 cut(s) 2679
BsaJI CCNNGG 7 cut(s) 261, 1604, 1935, 2479, 2669, 3057, 3258
BsaWI WCCGGW 2 cut(s) 1368, 2585
BsaXI ACNNNNNCTCC 4 cut(s) 2117, 2147, 2633, 2663
Bsc4I CCNNNNNNNGG 3 cut(s) 736, 1977, 3031
Bse1I ACTGG 1 cut(s) 707
Bse3DI GCAATG 1 cut(s) 2913
Bse8I GATNNNNATC 1 cut(s) 1557
BseBI CCWGG 4 cut(s) 955, 1172, 2639, 2670
BseDI CCNNGG 7 cut(s) 261, 1604, 1935, 2479, 2669, 3057, 3258
BseGI GGATG 6 cut(s) 121, 1499, 1515, 1749, 2244, 2457
BseJI GATNNNNATC 1 cut(s) 1557
BseLI CCNNNNNNNGG 3 cut(s) 736, 1977, 3031
BseMI GCAATG 1 cut(s) 2913
BseMII CTCAG 4 cut(s) 444, 1029, 2136, 2831
BseNI ACTGG 1 cut(s) 707
BseRI GAGGAG 5 cut(s) 803, 1817, 2138, 2657, 2737
BseSI GKGCMC 2 cut(s) 1156, 2042
BseXI GCAGC 7 cut(s) 391, 655, 1202, 1610, 2937, 2940, 3118
BseYI CCCAGC 1 cut(s) 2516
BsgI GTGCAG 2 cut(s) 654, 1609
Bsh1236I CGCG 2 cut(s) 78, 1376
Bsh1285I CGRYCG 2 cut(s) 82, 1380
BshFI GGCC 2 cut(s) 369, 422
BsiEI CGRYCG 2 cut(s) 82, 1380
BsiHKAI GWGCWC 2 cut(s) 2042, 2527
BsiSI CCGG 3 cut(s) 1369, 1519, 2586
BslFI GGGAC 2 cut(s) 2163, 2877
BslI CCNNNNNNNGG 3 cut(s) 736, 1977, 3031
BsmAI GTCTC 6 cut(s) 652, 785, 1829, 1920, 2679, 2740
BsmFI GGGAC 2 cut(s) 2163, 2877
BsmI GAATGC 1 cut(s) 1723
BsnI GGCC 2 cut(s) 369, 422
Bso31I GGTCTC 1 cut(s) 2679
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 5 cut(s) 1074, 1156, 1603, 2042, 2527
Bsp1407I TGTACA 1 cut(s) 2740
Bsp19I CCATGG 3 cut(s) 261, 1935, 2479
BspACI CCGC 6 cut(s) 496, 633, 2405, 2445, 2895, 2981
BspANI GGCC 2 cut(s) 369, 422
BspCNI CTCAG 4 cut(s) 443, 1028, 2137, 2832
BspFNI CGCG 2 cut(s) 78, 1376
BspHI TCATGA 1 cut(s) 831
BspLI GGNNCC 6 cut(s) 42, 1602, 2151, 2590, 2893, 3239
BspMAI CTGCAG 1 cut(s) 683
BspMI ACCTGC 2 cut(s) 2209, 2475
BspPI GGATC 4 cut(s) 296, 1729, 1788, 2343
BspQI GCTCTTC 1 cut(s) 799
BspT104I TTCGAA 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 1898
BspTNI GGTCTC 1 cut(s) 2679
BsrBI CCGCTC 1 cut(s) 2897
BsrDI GCAATG 1 cut(s) 2913
BsrGI TGTACA 1 cut(s) 2740
BsrI ACTGG 1 cut(s) 707
BssECI CCNNGG 7 cut(s) 261, 1604, 1935, 2479, 2669, 3057, 3258
BssNAI GTATAC 2 cut(s) 1265, 2323
BssNI GRCGYC 1 cut(s) 1989
BssSI CACGAG 1 cut(s) 2526
BssT1I CCWWGG 4 cut(s) 261, 1935, 2479, 3057
Bst1107I GTATAC 2 cut(s) 1265, 2323
Bst2BI CACGAG 1 cut(s) 2526
Bst2UI CCWGG 4 cut(s) 955, 1172, 2639, 2670
Bst4CI ACNGT 8 cut(s) 1441, 1931, 2114, 2441, 2512, 2942, 2987, 3070
Bst6I CTCTTC 6 cut(s) 323, 799, 1668, 2017, 2604, 3221
BstACI GRCGYC 1 cut(s) 1989
BstAFI CTTAAG 1 cut(s) 1898
BstAPI GCANNNNNTGC 1 cut(s) 2857
BstAUI TGTACA 1 cut(s) 2740
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 5 cut(s) 24, 679, 1469, 1661, 2948
BstDSI CCRYGG 4 cut(s) 261, 1604, 1935, 2479
BstF5I GGATG 6 cut(s) 121, 1499, 1515, 1749, 2244, 2457
BstFNI CGCG 2 cut(s) 78, 1376
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 1 cut(s) 589
BstMAI GTCTC 6 cut(s) 652, 785, 1829, 1920, 2679, 2740
BstMCI CGRYCG 2 cut(s) 82, 1380
BstMWI GCNNNNNNNGC 8 cut(s) 551, 633, 674, 806, 1139, 2522, 2857, 2903
BstNI CCWGG 4 cut(s) 955, 1172, 2639, 2670
BstNSI RCATGY 2 cut(s) 665, 2906
BstSCI CCNGG 5 cut(s) 953, 1170, 1518, 2637, 2668
BstSFI CTRYAG 1 cut(s) 679
BstSLI GKGCMC 2 cut(s) 1156, 2042
BstUI CGCG 2 cut(s) 78, 1376
BstV1I GCAGC 7 cut(s) 391, 655, 1202, 1610, 2937, 2940, 3118
BstV2I GAAGAC 4 cut(s) 273, 1344, 1365, 3071
BstX2I RGATCY 4 cut(s) 124, 1734, 1780, 2348
BstXI CCANNNNNNTGG 1 cut(s) 548
BstYI RGATCY 4 cut(s) 124, 1734, 1780, 2348
BstZ17I GTATAC 2 cut(s) 1265, 2323
BsuI GTATCC 3 cut(s) 133, 730, 1516
BsuRI GGCC 2 cut(s) 369, 422
BtgI CCRYGG 4 cut(s) 261, 1604, 1935, 2479
BtsCI GGATG 6 cut(s) 121, 1499, 1515, 1749, 2244, 2457
BtsI GCAGTG 3 cut(s) 505, 1626, 3057
BtsIMutI CAGTG 7 cut(s) 505, 700, 1446, 1626, 1637, 1791, 3057
BveI ACCTGC 2 cut(s) 2209, 2475
Cac8I GCNNGC 5 cut(s) 24, 679, 1469, 1661, 2948
CaiI CAGNNNCTG 3 cut(s) 800, 1929, 2516
CciI TCATGA 1 cut(s) 831
CfoI GCGC 1 cut(s) 589
Cfr13I GGNCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
CseI GACGC 1 cut(s) 2849
CspCI CAANNNNNGTGG 4 cut(s) 1063, 1098, 1593, 1628
DraI TTTAAA 4 cut(s) 778, 1590, 1854, 2775
DriI GACNNNNNGTC 1 cut(s) 1303
EaeI YGGCCR 2 cut(s) 367, 420
Eam1104I CTCTTC 6 cut(s) 323, 799, 1668, 2017, 2604, 3221
Eam1105I GACNNNNNGTC 1 cut(s) 1303
EarI CTCTTC 6 cut(s) 323, 799, 1668, 2017, 2604, 3221
Eco130I CCWWGG 4 cut(s) 261, 1935, 2479, 3057
Eco24I GRGCYC 2 cut(s) 1074, 1603
Eco31I GGTCTC 1 cut(s) 2679
Eco47I GGWCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
Eco57I CTGAAG 4 cut(s) 1569, 1689, 2651, 3104
EcoO109I RGGNCCY 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 524
EcoRII CCWGG 4 cut(s) 953, 1170, 2637, 2668
EcoT14I CCWWGG 4 cut(s) 261, 1935, 2479, 3057
EcoT22I ATGCAT 2 cut(s) 1509, 2853
EcoT38I GRGCYC 2 cut(s) 1074, 1603
ErhI CCWWGG 4 cut(s) 261, 1935, 2479, 3057
FalI AAGNNNNNCTT 4 cut(s) 1295, 1327, 2994, 3026
FaqI GGGAC 2 cut(s) 2163, 2877
FauI CCCGC 1 cut(s) 2902
FauNDI CATATG 1 cut(s) 2882
FblI GTMKAC 3 cut(s) 1123, 1264, 2322
Fnu4HI GCNGC 8 cut(s) 405, 634, 669, 1191, 1624, 2951, 2954, 3132
FokI GGATG 6 cut(s) 128, 1502, 1506, 1736, 2231, 2464
FriOI GRGCYC 2 cut(s) 1074, 1603
Fsp4HI GCNGC 8 cut(s) 405, 634, 669, 1191, 1624, 2951, 2954, 3132
FspBI CTAG 6 cut(s) 1202, 1464, 2429, 2768, 2864, 3277
GlaI GCGC 1 cut(s) 588
GluI GCNGC 8 cut(s) 405, 634, 669, 1191, 1624, 2951, 2954, 3132
GsaI CCCAGC 1 cut(s) 2520
GsuI CTGGAG 3 cut(s) 1679, 2660, 2780
HaeII RGCGCY 1 cut(s) 590
HaeIII GGCC 2 cut(s) 369, 422
HapII CCGG 3 cut(s) 1369, 1519, 2586
HgaI GACGC 1 cut(s) 2849
HhaI GCGC 1 cut(s) 589
Hin1I GRCGYC 1 cut(s) 1989
Hin6I GCGC 1 cut(s) 587
HinP1I GCGC 1 cut(s) 587
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 4 cut(s) 342, 1842, 1904, 3188
HpaII CCGG 3 cut(s) 1369, 1519, 2586
HphI GGTGA 6 cut(s) 667, 940, 1822, 2419, 2572, 2669
Hpy99I CGWCG 1 cut(s) 788
HpyAV CCTTC 6 cut(s) 577, 1180, 1613, 2807, 3162, 3235
HpyCH4III ACNGT 8 cut(s) 1441, 1931, 2114, 2441, 2512, 2942, 2987, 3070
HpyCH4IV ACGT 2 cut(s) 107, 1989
HpyF10VI GCNNNNNNNGC 8 cut(s) 551, 633, 674, 806, 1139, 2522, 2857, 2903
HpySE526I ACGT 2 cut(s) 107, 1989
Hsp92I GRCGYC 1 cut(s) 1989
HspAI GCGC 1 cut(s) 587
LguI GCTCTTC 1 cut(s) 799
LmnI GCTCC 4 cut(s) 551, 1606, 2366, 2902
Lsp1109I GCAGC 7 cut(s) 391, 655, 1202, 1610, 2937, 2940, 3118
LweI GCATC 7 cut(s) 505, 1390, 1494, 2512, 2626, 2838, 2978
MaeI CTAG 6 cut(s) 1202, 1464, 2429, 2768, 2864, 3277
MaeII ACGT 2 cut(s) 107, 1989
MaeIII GTNAC 7 cut(s) 1435, 1787, 2407, 2456, 2487, 2661, 3052
MbiI CCGCTC 1 cut(s) 2897
MfeI CAATTG 3 cut(s) 519, 2996, 3126
MflI RGATCY 4 cut(s) 124, 1734, 1780, 2348
MhlI GDGCHC 5 cut(s) 1074, 1156, 1603, 2042, 2527
MlsI TGGCCA 1 cut(s) 422
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 3 cut(s) 185, 451, 2926
MmeI TCCRAC 2 cut(s) 619, 3012
Mox20I TGGCCA 1 cut(s) 422
Mph1103I ATGCAT 2 cut(s) 1509, 2853
MroXI GAANNNNTTC 4 cut(s) 332, 744, 1060, 3006
MscI TGGCCA 1 cut(s) 422
MslI CAYNNNNRTG 1 cut(s) 2465
Msp20I TGGCCA 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 1898
MspI CCGG 3 cut(s) 1369, 1519, 2586
MspR9I CCNGG 5 cut(s) 955, 1172, 1520, 2639, 2670
MunI CAATTG 3 cut(s) 519, 2996, 3126
Mva1269I GAATGC 1 cut(s) 1723
MvaI CCWGG 4 cut(s) 955, 1172, 2639, 2670
MvnI CGCG 2 cut(s) 78, 1376
MwoI GCNNNNNNNGC 8 cut(s) 551, 633, 674, 806, 1139, 2522, 2857, 2903
NciI CCSGG 1 cut(s) 1520
NcoI CCATGG 3 cut(s) 261, 1935, 2479
NdeI CATATG 1 cut(s) 2882
NlaIV GGNNCC 6 cut(s) 42, 1602, 2151, 2590, 2893, 3239
NmeAIII GCCGAG 1 cut(s) 3239
NmuCI GTSAC 3 cut(s) 2407, 2456, 3052
NsiI ATGCAT 2 cut(s) 1509, 2853
NspI RCATGY 2 cut(s) 665, 2906
NspV TTCGAA 1 cut(s) 336
PagI TCATGA 1 cut(s) 831
PciI ACATGT 1 cut(s) 661
PciSI GCTCTTC 1 cut(s) 799
PcsI WCGNNNNNNNCGW 1 cut(s) 2817
PctI GAATGC 1 cut(s) 1723
PdmI GAANNNNTTC 4 cut(s) 332, 744, 1060, 3006
PfeI GAWTC 9 cut(s) 377, 391, 488, 901, 1025, 1756, 2010, 2531, 3103
PfoI TCCNGGA 1 cut(s) 1518
PkrI GCNGC 8 cut(s) 406, 635, 670, 1192, 1625, 2952, 2955, 3133
Ple19I CGATCG 2 cut(s) 82, 1380
PleI GAGTC 3 cut(s) 184, 451, 2925
PpsI GAGTC 3 cut(s) 184, 451, 2925
PpuMI RGGWCCY 1 cut(s) 41
PscI ACATGT 1 cut(s) 661
PsiI TTATAA 2 cut(s) 104, 453
Psp1406I AACGTT 1 cut(s) 107
Psp5II RGGWCCY 1 cut(s) 41
Psp6I CCWGG 4 cut(s) 953, 1170, 2637, 2668
PspFI CCCAGC 1 cut(s) 2516
PspGI CCWGG 4 cut(s) 953, 1170, 2637, 2668
PspN4I GGNNCC 6 cut(s) 42, 1602, 2151, 2590, 2893, 3239
PspPI GGNCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
PspPPI RGGWCCY 1 cut(s) 41
PstI CTGCAG 1 cut(s) 683
PstNI CAGNNNCTG 3 cut(s) 800, 1929, 2516
PsuI RGATCY 4 cut(s) 124, 1734, 1780, 2348
PvuI CGATCG 2 cut(s) 82, 1380
RseI CAYNNNNRTG 1 cut(s) 2465
SapI GCTCTTC 1 cut(s) 799
SatI GCNGC 8 cut(s) 405, 634, 669, 1191, 1624, 2951, 2954, 3132
Sau96I GGNCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
SchI GAGTC 3 cut(s) 185, 451, 2926
ScrFI CCNGG 5 cut(s) 955, 1172, 1520, 2639, 2670
SduI GDGCHC 5 cut(s) 1074, 1156, 1603, 2042, 2527
SfaNI GCATC 7 cut(s) 505, 1390, 1494, 2512, 2626, 2838, 2978
SfcI CTRYAG 1 cut(s) 679
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
SmiI ATTTAAAT 1 cut(s) 1854
SmiMI CAYNNNNRTG 1 cut(s) 2465
SmlI CTYRAG 3 cut(s) 1898, 2498, 2687
SmoI CTYRAG 3 cut(s) 1898, 2498, 2687
SsiI CCGC 6 cut(s) 496, 633, 2405, 2445, 2895, 2981
SspI AATATT 2 cut(s) 2170, 2214
SspMI CTAG 6 cut(s) 1202, 1464, 2429, 2768, 2864, 3277
StyD4I CCNGG 5 cut(s) 953, 1170, 1518, 2637, 2668
StyI CCWWGG 4 cut(s) 261, 1935, 2479, 3057
SwaI ATTTAAAT 1 cut(s) 1854
TaaI ACNGT 8 cut(s) 1441, 1931, 2114, 2441, 2512, 2942, 2987, 3070
TaiI ACGT 2 cut(s) 110, 1992
TaqI TCGA 7 cut(s) 82, 179, 316, 336, 786, 1396, 2820
TatI WGTACW 5 cut(s) 1723, 2109, 2433, 2544, 2740
TauI GCSGC 1 cut(s) 636
TfiI GAWTC 9 cut(s) 377, 391, 488, 901, 1025, 1756, 2010, 2531, 3103
TscAI CASTG 7 cut(s) 505, 707, 1446, 1633, 1644, 1798, 3057
TseFI GTSAC 3 cut(s) 2407, 2456, 3052
TseI GCWGC 7 cut(s) 404, 668, 1190, 1623, 2950, 2953, 3131
Tsp45I GTSAC 3 cut(s) 2407, 2456, 3052
TspRI CASTG 7 cut(s) 505, 707, 1446, 1633, 1644, 1798, 3057
Vha464I CTTAAG 1 cut(s) 1898
VneI GTGCAC 1 cut(s) 2038
VpaK11BI GGWCC 7 cut(s) 41, 1032, 1168, 2150, 2588, 2891, 3122
XbaI TCTAGA 1 cut(s) 2863
XceI RCATGY 2 cut(s) 665, 2906
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XmiI GTMKAC 3 cut(s) 1123, 1264, 2322
XmnI GAANNNNTTC 4 cut(s) 332, 744, 1060, 3006
XspI CTAG 6 cut(s) 1202, 1464, 2429, 2768, 2864, 3277
ZraI GACGTC 1 cut(s) 1990
Zsp2I ATGCAT 2 cut(s) 1509, 2853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.