MD15G1398500.v1.1
WRKY Family

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000379
Physical Location & Seq
Reverse (-)
49919665 .. 49921670
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1398500.v1.1.491

Sequence Viewer

Length: 1938 bp
ATGGCAGAGATTGTTTCTGGACTTGCGAGTCCGATAGTGCAGCTCGGTGAGTGGTTATGGATTCCAGTAAAGCGCGGGTTGAGTTACATGATTCACTACAAGAAAAACATACAGTCTCTTAAACTTCAAGTGGGAAAGCTCGAGGCAATGAAAAATGGTTATCAAGAAATCGTTCGTGCTGTTCAAACGAATGGAGAAGATGTCAAGCCTGAGATTCAAATATGGCTACAAGATGCTAATAAGGCCATAGCAGATGCAACACAATTAAACAATGAAGCCGAGGAAGCAGCTAAAAATTGCATCACCGGAAATTTGAGATGGCGTTACAGTTTAGGAAAGAAGGTAATGAAGAAGAAAGTAGCTTTAAACAAGCTACAAGAGGAGGGAAAGTTTGAAAGTATTTCAGTTCCAGTTATGCGGCCAATTGAAATCGAGTCCACAATGTCAACGAATGGTTTTCAAGCATTTGCAGCAACAAGACAAGCCATGGATCAAGTCATGAAAGCGCTGAAAGATGATAAGGTCACCGTCATTGGGGTCTATGGAATGGGAGGTGTGGGTAAGACAACCATGGTGAAAAATGTCGGTGTGCAAGCTTGTAAAGATGGACTCTTTGATCATGTGATTATGGCTGTCTTTACCCAAAACCCCAACTTGATGAAAATTCAAGGTCAGCTTTCAGACATGTTGGGCTTGAAATTGCAGGAGGAGACAGAATTGGGAAGAGCATGTAGACTGAGGAATAGGATACTAAGAGCAAAGAGGACGCTCATAATTTTAGATGACATTTGGAATACATTATTAGATTTGATCAGCATTGGGATTCCGAACCACACGGAGCTTCAAGGATGCAATTCCAAAGTCCTTCTCACCACAAGGAGATTAAATGTCTGTCATGCCATGGAAAGCCATGTCAAAATACCTCTCAACGTCCTATCTGAAGGAGATTCTTGGAACTTGTTTGTCAAGAAAGCGGGAAGGTCTTTTGAATCGCCTGATTTTAACAATATAGCGAGAAAGGTAGCTAGAGAATGTGCTGGTCTTCCGATAGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGACATTGACGAATGGAAAGAAGCAGCTCGACAACTTGAGATGTCCCGACCTGCCAACCTTGAAGACGAGGGGGATGTGCTCAGATGCATAAAGTTAAGCTATGATTACTTGAAAGGCGAGGATGCCAAGTCATGCTTCTTGCTTTGCTCCCTATTCCCAGAAGATGGTAAAATCCCAATACAAGACTTGTTTAATTATTGGTTTGGGAATGGATTGTTTCGAGATGGCAAGACACTGGAAGAAGCCAGAGCTAGGGCGCATTCAGTGATCAAGTACCTTAAAGCTTCTAGCTTGCTTTTGGATGGGGAAAGGAAAGAATATGTCAGGATGCATGATGTCATACGCGATATGGCCATATCAATTGCATATTCAGAACAGGGCCATGAGTTTTTAGTAAAAGCCGGTTGGGAATTGGAGGACTGGCCAAATGATGTCGATGAAGGCTGCTCTGCAATCTCACTAAAGAGCAACTGCATTTGCAAGCTTCCGGAAGAATTGGTATGTCCAAGACTCCGAATTTTACTTATAGATGATAATCTTAAACTAAAGAAGATCTCAGAAGCTTTTTTCCGAAGTCTGAATGCGTTAAGGGTCTTAGACCTTAGCAGCACTTGTATTTCGTCACTGCCCTCTTCGCTCAATCTCCTAACCAACCTTCAAACATTGCACCTAGATTCATGTTGGTCATTAAAGGATATCTCCATACTCAGAGAACTAACCAAACTTGAGATCCTTAGTCTGAAAGACTCACGTTTTGAGGAATTGCCAAAGGAAATAGGAAACTTGGCCAGTCTGAGGTTGTTGGATTTTGCTGGGAATACAATGATTCAGAGAGTTCCATCCAATAATTGA

Protein Analysis

646

Amino Acids

72.5

Weight (kDa)

7.81

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 161 - 327 2.3e-30 NB-ARC domain
WHD_DRP PF23559 413 - 480 2.8e-06 Disease resistance protein Winged helix domain
LRR_8 PF13855 531 - 589 6.5e-09 Leucine rich repeat
LRR_14 PF23598 594 - 643 1.9e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 1144
AccB7I CCANNNNNTGG 1 cut(s) 1250
AccI GTMKAC 1 cut(s) 733
AccII CGCG 2 cut(s) 75, 1431
AccIII TCCGGA 1 cut(s) 1573
AciI CCGC 3 cut(s) 75, 418, 974
AclWI GGATC 2 cut(s) 498, 1810
AcoI YGGCCR 4 cut(s) 419, 1437, 1508, 1872
AcsI RAATTY 3 cut(s) 310, 663, 1602
AcuI CTGAAG 1 cut(s) 960
AfaI GTAC 1 cut(s) 1361
AfeI AGCGCT 1 cut(s) 507
AfiI CCNNNNNNNGG 4 cut(s) 534, 1250, 1338, 1881
AflIII ACRYGT 1 cut(s) 684
AjuI GAANNNNNNNTTGG 2 cut(s) 1205, 1237
Alw21I GWGCWC 1 cut(s) 1167
Alw26I GTCTC 2 cut(s) 120, 704
AlwI GGATC 2 cut(s) 498, 1810
Ama87I CYCGRG 1 cut(s) 140
Aor13HI TCCGGA 1 cut(s) 1573
Aor51HI AGCGCT 1 cut(s) 507
AoxI GGCC 6 cut(s) 243, 419, 1437, 1465, 1508, 1872
ApeKI GCWGC 6 cut(s) 40, 287, 470, 1109, 1530, 1692
ApoI RAATTY 3 cut(s) 310, 663, 1602
ArsI GACNNNNNNTTYG 2 cut(s) 1823, 1855
Asp700I GAANNNNTTC 1 cut(s) 171
AspLEI GCGC 3 cut(s) 75, 508, 1345
AspS9I GGNCC 1 cut(s) 1465
AsuHPI GGTGA 5 cut(s) 59, 295, 517, 586, 862
AvaI CYCGRG 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 1075
BalI TGGCCA 3 cut(s) 1439, 1510, 1874
BbsI GAAGAC 2 cut(s) 1034, 1155
Bbv12I GWGCWC 1 cut(s) 1167
BbvI GCAGC 6 cut(s) 52, 299, 482, 1121, 1517, 1704
BccI CCATC 6 cut(s) 312, 599, 1244, 1304, 1382, 1933
BciVI GTATCC 1 cut(s) 741
BclI TGATCA 3 cut(s) 616, 810, 1353
BcoDI GTCTC 2 cut(s) 120, 704
BfaI CTAG 4 cut(s) 1026, 1338, 1374, 1757
BfoI RGCGCY 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 1144
BfuI GTATCC 1 cut(s) 741
BglII AGATCT 1 cut(s) 1638
BisI GCNGC 7 cut(s) 41, 288, 419, 471, 1110, 1531, 1693
BlsI GCNGC 7 cut(s) 42, 289, 420, 472, 1111, 1532, 1694
BmeT110I CYCGRG 1 cut(s) 140
BmgT120I GGNCC 1 cut(s) 1465
BmsI GCATC 7 cut(s) 223, 244, 309, 839, 1160, 1198, 1404
BpiI GAAGAC 2 cut(s) 1034, 1155
Bpu10I CCTNAGC 1 cut(s) 1688
BpuEI CTTGAG 2 cut(s) 1142, 1832
BsaBI GATNNNNATC 1 cut(s) 1620
BsaJI CCNNGG 4 cut(s) 279, 486, 570, 900
BsaWI WCCGGW 2 cut(s) 305, 1573
BsaXI ACNNNNNCTCC 4 cut(s) 186, 216, 374, 404
Bsc4I CCNNNNNNNGG 4 cut(s) 534, 1250, 1338, 1881
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 5 cut(s) 65, 410, 1326, 1511, 1875
Bse3DI GCAATG 2 cut(s) 153, 1748
Bse8I GATNNNNATC 1 cut(s) 1620
BseAI TCCGGA 1 cut(s) 1573
BseDI CCNNGG 4 cut(s) 279, 486, 570, 900
BseGI GGATG 6 cut(s) 854, 1165, 1213, 1393, 1419, 1925
BseJI GATNNNNATC 1 cut(s) 1620
BseLI CCNNNNNNNGG 4 cut(s) 534, 1250, 1338, 1881
BseMI GCAATG 2 cut(s) 153, 1748
BseMII CTCAG 6 cut(s) 201, 728, 1180, 1656, 1807, 1871
BseNI ACTGG 5 cut(s) 65, 410, 1326, 1511, 1875
BseRI GAGGAG 2 cut(s) 395, 722
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 6 cut(s) 52, 299, 482, 1121, 1517, 1704
BseYI CCCAGC 1 cut(s) 1898
BsgI GTGCAG 1 cut(s) 59
Bsh1236I CGCG 2 cut(s) 75, 1431
BshFI GGCC 6 cut(s) 245, 421, 1439, 1467, 1510, 1874
BsiHKAI GWGCWC 1 cut(s) 1167
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 3 cut(s) 306, 1488, 1574
BslFI GGGAC 1 cut(s) 1114
BslI CCNNNNNNNGG 4 cut(s) 534, 1250, 1338, 1881
BsmAI GTCTC 2 cut(s) 120, 704
BsmFI GGGAC 1 cut(s) 1114
BsmI GAATGC 2 cut(s) 1345, 1672
BsnI GGCC 6 cut(s) 245, 421, 1439, 1467, 1510, 1874
BsoBI CYCGRG 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 1075, 1167
Bsp13I TCCGGA 1 cut(s) 1573
Bsp143I GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
Bsp19I CCATGG 3 cut(s) 486, 570, 900
BspACI CCGC 3 cut(s) 75, 418, 974
BspANI GGCC 6 cut(s) 245, 421, 1439, 1467, 1510, 1874
BspCNI CTCAG 6 cut(s) 202, 729, 1179, 1655, 1806, 1872
BspEI TCCGGA 1 cut(s) 1573
BspFNI CGCG 2 cut(s) 75, 1431
BspHI TCATGA 1 cut(s) 498
BspMI ACCTGC 1 cut(s) 1144
BspPI GGATC 2 cut(s) 498, 1810
BspQI GCTCTTC 1 cut(s) 718
BsrDI GCAATG 2 cut(s) 153, 1748
BsrFI RCCGGY 1 cut(s) 1487
BsrI ACTGG 5 cut(s) 65, 410, 1326, 1511, 1875
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 4 cut(s) 279, 486, 570, 900
BssMI GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
BssT1I CCWWGG 3 cut(s) 486, 570, 900
Bst4CI ACNGT 3 cut(s) 114, 329, 529
Bst6I CTCTTC 2 cut(s) 718, 1723
BstC8I GCNNGC 3 cut(s) 594, 1379, 1568
BstDSI CCRYGG 3 cut(s) 486, 570, 900
BstEII GGTNACC 1 cut(s) 523
BstF5I GGATG 6 cut(s) 854, 1165, 1213, 1393, 1419, 1925
BstFNI CGCG 2 cut(s) 75, 1431
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 3 cut(s) 75, 508, 1345
BstKTI GATC 6 cut(s) 493, 619, 813, 1356, 1641, 1818
BstMAI GTCTC 2 cut(s) 120, 704
BstMBI GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
BstMWI GCNNNNNNNGC 5 cut(s) 242, 284, 470, 1058, 1720
BstNSI RCATGY 2 cut(s) 688, 732
BstPI GGTNACC 1 cut(s) 523
BstSLI GKGCMC 1 cut(s) 1075
BstUI CGCG 2 cut(s) 75, 1431
BstV1I GCAGC 6 cut(s) 52, 299, 482, 1121, 1517, 1704
BstV2I GAAGAC 2 cut(s) 1034, 1155
BstX2I RGATCY 2 cut(s) 1638, 1815
BstYI RGATCY 2 cut(s) 1638, 1815
BsuI GTATCC 1 cut(s) 741
BsuRI GGCC 6 cut(s) 245, 421, 1439, 1467, 1510, 1874
BtgI CCRYGG 3 cut(s) 486, 570, 900
BtsCI GGATG 6 cut(s) 854, 1165, 1213, 1393, 1419, 1925
BtsI GCAGTG 1 cut(s) 1709
BtsIMutI CAGTG 3 cut(s) 1319, 1356, 1709
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 3 cut(s) 594, 1379, 1568
CciI TCATGA 1 cut(s) 498
CfoI GCGC 3 cut(s) 75, 508, 1345
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 1 cut(s) 1465
CseI GACGC 1 cut(s) 775
Csp6I GTAC 1 cut(s) 1360
CviQI GTAC 1 cut(s) 1360
DpnI GATC 6 cut(s) 492, 618, 812, 1355, 1640, 1817
DpnII GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
DraI TTTAAA 1 cut(s) 366
DrdI GACNNNNNNGTC 1 cut(s) 27
DseDI GACNNNNNNGTC 1 cut(s) 27
EaeI YGGCCR 4 cut(s) 419, 1437, 1508, 1872
Eam1104I CTCTTC 2 cut(s) 718, 1723
EarI CTCTTC 2 cut(s) 718, 1723
Eco130I CCWWGG 3 cut(s) 486, 570, 900
Eco32I GATATC 1 cut(s) 1783
Eco47III AGCGCT 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 960
Eco88I CYCGRG 1 cut(s) 140
Eco91I GGTNACC 1 cut(s) 523
EcoO65I GGTNACC 1 cut(s) 523
EcoRV GATATC 1 cut(s) 1783
EcoT14I CCWWGG 3 cut(s) 486, 570, 900
EcoT22I ATGCAT 2 cut(s) 1175, 1419
ErhI CCWWGG 3 cut(s) 486, 570, 900
FalI AAGNNNNNCTT 4 cut(s) 660, 692, 1205, 1237
FaqI GGGAC 1 cut(s) 1114
FauI CCCGC 2 cut(s) 68, 967
FbaI TGATCA 3 cut(s) 616, 810, 1353
FblI GTMKAC 1 cut(s) 733
Fnu4HI GCNGC 7 cut(s) 41, 288, 419, 471, 1110, 1531, 1693
FokI GGATG 6 cut(s) 861, 1172, 1220, 1400, 1426, 1912
Fsp4HI GCNGC 7 cut(s) 41, 288, 419, 471, 1110, 1531, 1693
FspBI CTAG 4 cut(s) 1026, 1338, 1374, 1757
GlaI GCGC 3 cut(s) 74, 507, 1344
GluI GCNGC 7 cut(s) 41, 288, 419, 471, 1110, 1531, 1693
GsaI CCCAGC 1 cut(s) 1902
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 6 cut(s) 245, 421, 1439, 1467, 1510, 1874
HapII CCGG 3 cut(s) 306, 1488, 1574
HgaI GACGC 1 cut(s) 775
HhaI GCGC 3 cut(s) 75, 508, 1345
Hin6I GCGC 3 cut(s) 73, 506, 1343
HinP1I GCGC 3 cut(s) 73, 506, 1343
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HindIII AAGCTT 4 cut(s) 594, 1368, 1568, 1647
HpaII CCGG 3 cut(s) 306, 1488, 1574
HphI GGTGA 5 cut(s) 59, 295, 517, 586, 862
Hpy166II GTNNAC 3 cut(s) 438, 447, 734
Hpy188III TCNNGA 8 cut(s) 18, 164, 499, 967, 1131, 1307, 1411, 1574
Hpy8I GTNNAC 3 cut(s) 438, 447, 734
HpyAV CCTTC 6 cut(s) 334, 875, 935, 972, 1520, 1751
HpyCH4III ACNGT 3 cut(s) 114, 329, 529
HpyCH4IV ACGT 2 cut(s) 930, 1837
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 284, 470, 1058, 1720
HpySE526I ACGT 2 cut(s) 930, 1837
HspAI GCGC 3 cut(s) 73, 506, 1343
Kpn2I TCCGGA 1 cut(s) 1573
Ksp22I TGATCA 3 cut(s) 616, 810, 1353
Kzo9I GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
LguI GCTCTTC 1 cut(s) 718
LmnI GCTCC 2 cut(s) 838, 1238
Lsp1109I GCAGC 6 cut(s) 52, 299, 482, 1121, 1517, 1704
LweI GCATC 7 cut(s) 223, 244, 309, 839, 1160, 1198, 1404
MaeI CTAG 4 cut(s) 1026, 1338, 1374, 1757
MaeII ACGT 2 cut(s) 930, 1837
MaeIII GTNAC 4 cut(s) 83, 323, 523, 1707
MalI GATC 6 cut(s) 492, 618, 812, 1355, 1640, 1817
MboI GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
MfeI CAATTG 2 cut(s) 423, 1446
MflI RGATCY 2 cut(s) 1638, 1815
MhlI GDGCHC 2 cut(s) 1075, 1167
MlsI TGGCCA 3 cut(s) 1439, 1510, 1874
MluNI TGGCCA 3 cut(s) 1439, 1510, 1874
MlyI GAGTC 5 cut(s) 37, 443, 603, 1590, 1826
MmeI TCCRAC 1 cut(s) 1869
Mox20I TGGCCA 3 cut(s) 1439, 1510, 1874
Mph1103I ATGCAT 2 cut(s) 1175, 1419
MroI TCCGGA 1 cut(s) 1573
MroXI GAANNNNTTC 1 cut(s) 171
MscI TGGCCA 3 cut(s) 1439, 1510, 1874
Msp20I TGGCCA 3 cut(s) 1439, 1510, 1874
MspI CCGG 3 cut(s) 306, 1488, 1574
MunI CAATTG 2 cut(s) 423, 1446
Mva1269I GAATGC 2 cut(s) 1345, 1672
MvnI CGCG 2 cut(s) 75, 1431
MwoI GCNNNNNNNGC 5 cut(s) 242, 284, 470, 1058, 1720
NcoI CCATGG 3 cut(s) 486, 570, 900
NdeII GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 523, 1707
NsiI ATGCAT 2 cut(s) 1175, 1419
NspI RCATGY 2 cut(s) 688, 732
PaeR7I CTCGAG 1 cut(s) 140
PagI TCATGA 1 cut(s) 498
PciI ACATGT 1 cut(s) 684
PciSI GCTCTTC 1 cut(s) 718
PctI GAATGC 2 cut(s) 1345, 1672
PdmI GAANNNNTTC 1 cut(s) 171
PfeI GAWTC 8 cut(s) 61, 91, 214, 823, 947, 989, 1760, 1912
PflMI CCANNNNNTGG 1 cut(s) 1250
PkrI GCNGC 7 cut(s) 42, 289, 420, 472, 1111, 1532, 1694
PleI GAGTC 5 cut(s) 36, 442, 603, 1590, 1826
PpsI GAGTC 5 cut(s) 36, 442, 603, 1590, 1826
PscI ACATGT 1 cut(s) 684
PspEI GGTNACC 1 cut(s) 523
PspFI CCCAGC 1 cut(s) 1898
PspPI GGNCC 1 cut(s) 1465
PspXI VCTCGAGB 1 cut(s) 140
PsuI RGATCY 2 cut(s) 1638, 1815
RsaI GTAC 1 cut(s) 1361
RsaNI GTAC 1 cut(s) 1360
SapI GCTCTTC 1 cut(s) 718
SatI GCNGC 7 cut(s) 41, 288, 419, 471, 1110, 1531, 1693
Sau3AI GATC 6 cut(s) 490, 616, 810, 1353, 1638, 1815
Sau96I GGNCC 1 cut(s) 1465
SchI GAGTC 5 cut(s) 37, 443, 603, 1590, 1826
SduI GDGCHC 2 cut(s) 1075, 1167
SfaNI GCATC 7 cut(s) 223, 244, 309, 839, 1160, 1198, 1404
Sfr274I CTCGAG 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 3 cut(s) 140, 1121, 1811
SmoI CTYRAG 3 cut(s) 140, 1121, 1811
SsiI CCGC 3 cut(s) 75, 418, 974
SspMI CTAG 4 cut(s) 1026, 1338, 1374, 1757
StyI CCWWGG 3 cut(s) 486, 570, 900
TaaI ACNGT 3 cut(s) 114, 329, 529
TaiI ACGT 2 cut(s) 933, 1840
TaqI TCGA 5 cut(s) 141, 432, 1114, 1306, 1521
TauI GCSGC 1 cut(s) 421
TfiI GAWTC 8 cut(s) 61, 91, 214, 823, 947, 989, 1760, 1912
TscAI CASTG 3 cut(s) 1326, 1356, 1716
TseFI GTSAC 2 cut(s) 523, 1707
TseI GCWGC 6 cut(s) 40, 287, 470, 1109, 1530, 1692
Tsp45I GTSAC 2 cut(s) 523, 1707
TspDTI ATGAA 7 cut(s) 164, 288, 362, 515, 674, 1539, 1752
TspGWI ACGGA 1 cut(s) 851
TspRI CASTG 3 cut(s) 1326, 1356, 1716
Van91I CCANNNNNTGG 1 cut(s) 1250
XapI RAATTY 3 cut(s) 310, 663, 1602
XceI RCATGY 2 cut(s) 688, 732
XhoI CTCGAG 1 cut(s) 140
XmiI GTMKAC 1 cut(s) 733
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 4 cut(s) 1026, 1338, 1374, 1757
Zsp2I ATGCAT 2 cut(s) 1175, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.