FvH4_3g44680

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37213235 .. 37221318
8084 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44680.t2

Sequence Viewer

Length: 3576 bp
ATGGATAGACTCCCATTAATGCCCACATTGCCTTTCCTACAGCTTGAGGATTGGAACCTGATTAACAAACATGATGGTTACGGAGGCCTGTTTCAGCTACATACACATCAAGGCTGGTTATCTAGCTGGGAACTCGTATGTGATGGACAAAAAGTTGCTATAGGCATCCTAACTGAGAGTGAAGTGATCAGTTGGCATGCTCTGGGAAATGTCTCAAGGTATTCTTGCCGCTGGGTTTGTATTCTTACCAGTTTCCATTGCAGGTTTTCAATGGCCTCCTCTTCATCATCGTCAACTCGCAGCTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTACTGACACTCGCAAAGGGATTGCAAACGAGATCTGCAATCGACTTAGTAGTAGAGGAATCAAAACGTTCATGGATGAGCAAGGACTTGAAGTAGGGGATGTTATTTCACCCACCCTCATAGAGGCAATTAAAGAGTCAAGGTTAGCAATCGTAATTCTCTCGCAAAATTACGCTTCTTCTGCTTGGTGTTTGGAGGAACTTCGAGAGATTTGTCTATCCATGGAAGACAACAGAATTTTGCCACTTTTTTATCATGTTGATCCTACTAATGTTCGACATCAGACGGGAAGTTTCAGAGATGCTTTGTCTAAGCACGAAACCTCTCAGCGACATGAATCAGAGAAGATGAATCAATGGAGGGCTGCCTTAAGCAAAGTGGCCAATATCTCAGGGTGGAATACAAATGATTTCAAGAGTCACAGTGAACTTGTGGACAAGATTGTGGAATCTCTCCGCAGTAAAGTATCACTTGATCCAATTGAATCCACAGGAAATTTCCAAGCATATGAAGCAACAAAAGAAGCCATGCGCAAGGTTATGGAGGCGCTAACAGATGACAAGATTACTGTCATTGGTGTCCACGGCATGGGCGGCGTTGGGAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCAGTAAAAATGGTACTTTTAACCATTGGATTATTGCTGTCGTATCCCAAAACCTTGACTTGAAAATAGTTCAAGGTACATTGGCAGATTCGTTGGGCTTTAAATTCGTGGAGGAGACACAAGCTGGAAGAGCTGATAGGTTGCATAAGGAGCTAATGGGAAAAGAAAAGCTCCTTATAATCTTGGACGATGTATGGGATAAAATAAAGTTGTCAAAGATTGGGATCCCTGACTACAAAGAGCTTCAAACATGCAATTCCAAAGTCCTACTCACCACAAGGAGATTGAGTGTCTGTAATGCCATGGAGTGCCATGAAAAAATCGCCCTCAACATTCTATCAAAAGAAGATTCTTGGGCCTTGTTTGTGAGAAATGTAGGAACAATTTCTTTTGAGTCCACCTCGTTTAAGGAAGTGGCATGGAGGGTAGCTGGAGAGTGTAAGGGTCTACCGATTGCATTGAAAGTTGTTGGAAGGGCACTCAGAGGTGAGGACCAGGAGGAATGGGAGAAGGCAGCTAAACGACTAGAGCAATCGAAATATGTCAATCCCAGCCATGAGGAAGATGAGGAAAACGCATTCCAATGTATAAGATTAAGCTATAATTACTTGAAAGATGGGGATTCCAAGTCATGCTTCTTGCTTTGTTGCCTGTTCCCAGAAGACGATGACATCGAAATAGAAGACTTGTTCCGGTACACGATCGGAAAAAGATTGTTTCGAGATGCCGATACAATGGAAGAAGCCAGAGGAATAGTAGTTACAGTGGTCAAGTACTTGAAAAATTCTGGCTTGCTTTTGGATAGCGAGAAAAAAGGATGTGTAAAGATGCATGATGTCATCCGAGATACAGCCCTGAATATTGCACAATCTGAAGATGGGCATCGGTTTTTTGTGAAAGCTGGCTGTGGTTTAAAGGCTTGGCCACCACGCGGATTACATCAAGGCTGCACTGCTATTTCACTAATGAGGAACGAAATTCGCAAGCCACCCGAAGCGTTGATATGTCCAAATCTCCAGATTTTATTACTAAACGGGAATGCTGAACTAAGTGAGATCCCAGAAAAGTTGATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGCAACACTGGTATTTCCGTATTACCCCAATCATTTAGTCTCCTCACAAACCTCCAAGCTTTGTATTTAGATCATTGCCGCAAATTAATTGACATTTCCATAGTGGGGAAACTTAAGAAGCTTGAAATTCTTAGTATGAGAGACTGTCCCAAGGGGCAATTGTCTAGAGAAATAGAACAGTTGACCAATCTAAGGATTTTGGATGTCAATCGCACTTGGGCAGCGGGTGGTATGGTTACAATTCCCTCTAAAGTGATACCAAAGTTGCATAAATTAGAAGAATTGTATATGGTGTACTGTGGATTTGAGGATGGGTGGTATGTAGCTGAGGGACCAAGAGAAAGAACCAATATTATTAGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAATAAATTGCAGCTTGGCTTATCCGATGAAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACTTTTGTATAATTATCTACGGCAGATGCACCAGCTCTATATTCAGAGAAGAAGATCGTAATTCAAGATTCTTGTTTCTTAGTGGAGCAACCATTAGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATTACTGCTGCGGAGGGATGAGTGACATTGTTATGGAATATGACCACGGGAGGTTACATAAACTCAAGCATCTCACAGTTGCTAGGCAACATGACGAGTTGAAAGAGTTGATGAACACAACAAGACGAGTTGAAACAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGATAGAACTGATCCACCTGGAGGAGTTGTGTGTTGGTGAGCTACCACCTCGGTCTCTCTCAAATCTCAAGGTGTTTAATGTGTTTTTAGGTGAGAGTTTGAAGAGTGTATCAGAATTTGTACAGAGACTACCAAATCTGGAGAAACTAGATTTAAACTATACGCCCAAATTAGAATATGTGTTCAGATGCGAAGGGTTCGAGCCAGAACAGTCAAAACTGAGAGAGATGCATTTGTTGGGTCCAAATTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGTGCAATGTTCCAGAGTCTTAAGATTTTGACCATTTACAGTTGTGATCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTCAGTGTCTTGTTCAATTGGAAGACATTCTTGTAGAGGATTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGGTTCTACCAAAACTGAAGAACTTGGTTTTGAGGCATCTTCCTATTTTGTATGGTGCAATTGCTGCTGTTGATATTGAGTGTCCTTCATTGGAACACTTGATGGTGGTGTATTGCCCCAAGCTTTTATTTTCAACCTCTTCCGATCTTTTCGAAGCCTTCAAAAGCAGAAACCAATTTTCATTTTCAGCCTCCACTTCTGACCCAGTCCAACTCAATGATCCACAACTATACCAAACTCTACATTGCAGAACATCTGGTTTCTGA

Protein Analysis

1192

Amino Acids

135.06

Weight (kDa)

5.98

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 90 - 254 4.3e-48 TIR domain
TIR_2 PF13676 93 - 188 2.5e-15 TIR domain
NB-ARC PF00931 272 - 437 2.9e-32 NB-ARC domain
LRR_8 PF13855 647 - 706 4.1e-10 Leucine rich repeat
LRR_14 PF23598 652 - 783 2.5e-17 Leucine-rich repeat region
LRR_RPS2 PF23247 937 - 1076 4.1e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1017 - 1132 4.4e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1148
Acc16I TGCGCA 1 cut(s) 866
Acc36I ACCTGC 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 922
AccI GTMKAC 1 cut(s) 1417
AccII CGCG 1 cut(s) 1902
AciI CCGC 8 cut(s) 229, 790, 927, 1902, 2155, 2300, 2663, 2703
AclI AACGTT 1 cut(s) 401
AclWI GGATC 9 cut(s) 590, 803, 1189, 1202, 2020, 2035, 2079, 2869, 3524
AcoI YGGCCR 2 cut(s) 714, 1892
AcuI CTGAAG 2 cut(s) 1863, 3347
AcvI CACGTG 1 cut(s) 3146
AfaI GTAC 8 cut(s) 337, 985, 1048, 1667, 1745, 2372, 2638, 2985
AfiI CCNNNNNNNGG 7 cut(s) 457, 922, 1901, 2181, 2268, 2884, 3274
AflII CTTAAG 3 cut(s) 703, 2189, 3164
AflIII ACRYGT 1 cut(s) 955
AjnI CCWGG 2 cut(s) 1464, 2880
AjuI GAANNNNNNNTTGG 2 cut(s) 1589, 1621
Alw26I GTCTC 7 cut(s) 217, 946, 1079, 2120, 2211, 2922, 2983
AlwI GGATC 9 cut(s) 590, 803, 1189, 1202, 2020, 2035, 2079, 2869, 3524
AlwNI CAGNNNCTG 1 cut(s) 2050
AoxI GGCC 5 cut(s) 85, 273, 714, 1326, 1892
AseI ATTAAT 2 cut(s) 17, 2162
Asp700I GAANNNNTTC 2 cut(s) 1354, 3249
AspLEI GCGC 2 cut(s) 867, 883
AspS9I GGNCC 6 cut(s) 1326, 1462, 2408, 2831, 3104, 3134
AsuHPI GGTGA 7 cut(s) 435, 961, 1234, 1469, 2677, 2912, 2966
AsuII TTCGAA 1 cut(s) 3462
AvaII GGWCC 5 cut(s) 1462, 2408, 2831, 3104, 3134
BaeGI GKGCMC 1 cut(s) 1450
BaeI ACNNNNGTAYC 2 cut(s) 2324, 2357
BalI TGGCCA 2 cut(s) 716, 1894
BamHI GGATCC 1 cut(s) 1194
BbrPI CACGTG 1 cut(s) 3146
BbsI GAAGAC 6 cut(s) 567, 944, 1638, 1659, 3252, 3314
BbvCI CCTCAGC 1 cut(s) 2403
BccI CCATC 6 cut(s) 68, 137, 1580, 1841, 2381, 3406
BceAI ACGGC 2 cut(s) 934, 2575
BciT130I CCWGG 2 cut(s) 1466, 2882
BciVI GTATCC 1 cut(s) 1024
BclI TGATCA 1 cut(s) 186
BcoDI GTCTC 7 cut(s) 217, 946, 1079, 2120, 2211, 2922, 2983
BfaI CTAG 6 cut(s) 123, 1496, 2241, 2687, 2775, 3011
BfmI CTRYAG 2 cut(s) 38, 159
BfoI RGCGCY 1 cut(s) 884
BfrI CTTAAG 3 cut(s) 703, 2189, 3164
BfuAI ACCTGC 1 cut(s) 252
BfuI GTATCC 1 cut(s) 1024
BglII AGATCT 1 cut(s) 366
BmcAI AGTACT 1 cut(s) 1745
Bme1390I CCNGG 2 cut(s) 1466, 2882
Bme18I GGWCC 5 cut(s) 1462, 2408, 2831, 3104, 3134
BmgT120I GGNCC 6 cut(s) 1326, 1462, 2408, 2831, 3104, 3134
BmiI GGNNCC 4 cut(s) 56, 1196, 2409, 3105
BmrFI CCNGG 2 cut(s) 1466, 2882
BmrI ACTGGG 1 cut(s) 3509
BmuI ACTGGG 1 cut(s) 3509
BpiI GAAGAC 6 cut(s) 567, 944, 1638, 1659, 3252, 3314
BplI GAGNNNNNCTC 2 cut(s) 1355, 1387
BpmI CTGGAG 4 cut(s) 1422, 1970, 2903, 3023
Bpu10I CCTNAGC 1 cut(s) 2403
Bpu14I TTCGAA 1 cut(s) 3462
BpuEI CTTGAG 4 cut(s) 65, 199, 2741, 2915
BsaAI YACGTR 1 cut(s) 3146
BsaBI GATNNNNATC 3 cut(s) 1193, 1851, 2283
BsaI GGTCTC 1 cut(s) 2922
BsaJI CCNNGG 6 cut(s) 555, 916, 1272, 2226, 2737, 2912
BsaWI WCCGGW 1 cut(s) 1662
BsaXI ACNNNNNCTCC 4 cut(s) 1243, 1273, 2876, 2906
Bsc4I CCNNNNNNNGG 7 cut(s) 457, 922, 1901, 2181, 2268, 2884, 3274
Bse1I ACTGG 5 cut(s) 249, 972, 2089, 2834, 3515
Bse3DI GCAATG 5 cut(s) 26, 256, 2149, 3156, 3553
Bse8I GATNNNNATC 3 cut(s) 1193, 1851, 2283
BseBI CCWGG 2 cut(s) 1466, 2882
BseDI CCNNGG 6 cut(s) 555, 916, 1272, 2226, 2737, 2912
BseGI GGATG 9 cut(s) 165, 415, 439, 1793, 1809, 2284, 2392, 2502, 2715
BseJI GATNNNNATC 3 cut(s) 1193, 1851, 2283
BseLI CCNNNNNNNGG 7 cut(s) 457, 922, 1901, 2181, 2268, 2884, 3274
BseMI GCAATG 5 cut(s) 26, 256, 2149, 3156, 3553
BseMII CTCAG 7 cut(s) 165, 674, 738, 1465, 2394, 3074, 3239
BseNI ACTGG 5 cut(s) 249, 972, 2089, 2834, 3515
BseRI GAGGAG 4 cut(s) 268, 1097, 2108, 2900
BseSI GKGCMC 1 cut(s) 1450
BseYI CCCAGC 3 cut(s) 126, 231, 1520
BsgI GTGCAG 2 cut(s) 948, 1903
Bsh1236I CGCG 1 cut(s) 1902
Bsh1285I CGRYCG 1 cut(s) 1674
BshFI GGCC 5 cut(s) 87, 275, 716, 1328, 1894
BsiEI CGRYCG 1 cut(s) 1674
BsiSI CCGG 1 cut(s) 1663
BslFI GGGAC 2 cut(s) 2208, 2421
BslI CCNNNNNNNGG 7 cut(s) 457, 922, 1901, 2181, 2268, 2884, 3274
BsmAI GTCTC 7 cut(s) 217, 946, 1079, 2120, 2211, 2922, 2983
BsmFI GGGAC 2 cut(s) 2208, 2421
BsmI GAATGC 2 cut(s) 1547, 2014
BsnI GGCC 5 cut(s) 87, 275, 716, 1328, 1894
Bso31I GGTCTC 1 cut(s) 2922
Bsp119I TTCGAA 1 cut(s) 3462
Bsp1286I GDGCHC 1 cut(s) 1450
Bsp1407I TGTACA 1 cut(s) 2983
Bsp19I CCATGG 2 cut(s) 555, 1272
BspACI CCGC 8 cut(s) 229, 790, 927, 1902, 2155, 2300, 2663, 2703
BspANI GGCC 5 cut(s) 87, 275, 716, 1328, 1894
BspCNI CTCAG 7 cut(s) 166, 673, 737, 1464, 2395, 3075, 3238
BspFNI CGCG 1 cut(s) 1902
BspLI GGNNCC 4 cut(s) 56, 1196, 2409, 3105
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 9 cut(s) 590, 803, 1189, 1202, 2020, 2035, 2079, 2869, 3524
BspQI GCTCTTC 2 cut(s) 1093, 3113
BspT104I TTCGAA 1 cut(s) 3462
BspTI CTTAAG 3 cut(s) 703, 2189, 3164
BspTNI GGTCTC 1 cut(s) 2922
BsrDI GCAATG 5 cut(s) 26, 256, 2149, 3156, 3553
BsrGI TGTACA 1 cut(s) 2983
BsrI ACTGG 5 cut(s) 249, 972, 2089, 2834, 3515
BssECI CCNNGG 6 cut(s) 555, 916, 1272, 2226, 2737, 2912
BssT1I CCWWGG 3 cut(s) 555, 1272, 2226
Bst2UI CCWGG 2 cut(s) 1466, 2882
Bst6I CTCTTC 6 cut(s) 286, 1093, 2847, 2960, 3113, 3454
BstAFI CTTAAG 3 cut(s) 703, 2189, 3164
BstAPI GCANNNNNTGC 1 cut(s) 3374
BstAUI TGTACA 1 cut(s) 2983
BstBAI YACGTR 1 cut(s) 3146
BstBI TTCGAA 1 cut(s) 3462
BstC8I GCNNGC 4 cut(s) 198, 1763, 1873, 1955
BstDSI CCRYGG 4 cut(s) 555, 916, 1272, 2737
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 9 cut(s) 165, 415, 439, 1793, 1809, 2284, 2392, 2502, 2715
BstFNI CGCG 1 cut(s) 1902
BstH2I RGCGCY 1 cut(s) 884
BstHHI GCGC 2 cut(s) 867, 883
BstMAI GTCTC 7 cut(s) 217, 946, 1079, 2120, 2211, 2922, 2983
BstMCI CGRYCG 1 cut(s) 1674
BstMWI GCNNNNNNNGC 9 cut(s) 28, 515, 845, 927, 968, 1100, 1120, 3146, 3374
BstNI CCWGG 2 cut(s) 1466, 2882
BstNSI RCATGY 3 cut(s) 200, 959, 1224
BstSCI CCNGG 2 cut(s) 1464, 2880
BstSFI CTRYAG 2 cut(s) 38, 159
BstSLI GKGCMC 1 cut(s) 1450
BstUI CGCG 1 cut(s) 1902
BstV2I GAAGAC 6 cut(s) 567, 944, 1638, 1659, 3252, 3314
BstX2I RGATCY 4 cut(s) 366, 1194, 2025, 2071
BstYI RGATCY 4 cut(s) 366, 1194, 2025, 2071
BsuI GTATCC 1 cut(s) 1024
BsuRI GGCC 5 cut(s) 87, 275, 716, 1328, 1894
BtgI CCRYGG 4 cut(s) 555, 916, 1272, 2737
BtsCI GGATG 9 cut(s) 165, 415, 439, 1793, 1809, 2284, 2392, 2502, 2715
BtsI GCAGTG 2 cut(s) 1920, 3300
BtsIMutI CAGTG 8 cut(s) 763, 1740, 1920, 2082, 2841, 3120, 3233, 3300
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 4 cut(s) 198, 1763, 1873, 1955
CaiI CAGNNNCTG 1 cut(s) 2050
CfoI GCGC 2 cut(s) 867, 883
Cfr13I GGNCC 6 cut(s) 1326, 1462, 2408, 2831, 3104, 3134
Csp6I GTAC 8 cut(s) 336, 984, 1047, 1666, 1744, 2371, 2637, 2984
CviQI GTAC 8 cut(s) 336, 984, 1047, 1666, 1744, 2371, 2637, 2984
DraI TTTAAA 3 cut(s) 1072, 1884, 3018
EaeI YGGCCR 2 cut(s) 714, 1892
Eam1104I CTCTTC 6 cut(s) 286, 1093, 2847, 2960, 3113, 3454
EarI CTCTTC 6 cut(s) 286, 1093, 2847, 2960, 3113, 3454
Eco130I CCWWGG 3 cut(s) 555, 1272, 2226
Eco147I AGGCCT 1 cut(s) 87
Eco31I GGTCTC 1 cut(s) 2922
Eco47I GGWCC 5 cut(s) 1462, 2408, 2831, 3104, 3134
Eco57I CTGAAG 2 cut(s) 1863, 3347
Eco72I CACGTG 1 cut(s) 3146
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 1464, 2880
EcoT14I CCWWGG 3 cut(s) 555, 1272, 2226
EcoT22I ATGCAT 2 cut(s) 1803, 3096
ErhI CCWWGG 3 cut(s) 555, 1272, 2226
FalI AAGNNNNNCTT 8 cut(s) 208, 240, 789, 821, 1589, 1621, 3237, 3269
FaqI GGGAC 2 cut(s) 2208, 2421
FauI CCCGC 1 cut(s) 2293
FauNDI CATATG 1 cut(s) 841
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 1 cut(s) 1417
FokI GGATG 9 cut(s) 152, 422, 446, 1796, 1800, 2291, 2399, 2489, 2722
FspBI CTAG 6 cut(s) 123, 1496, 2241, 2687, 2775, 3011
FspI TGCGCA 1 cut(s) 866
GlaI GCGC 2 cut(s) 866, 882
GsaI CCCAGC 3 cut(s) 130, 235, 1524
GsuI CTGGAG 4 cut(s) 1422, 1970, 2903, 3023
HaeII RGCGCY 1 cut(s) 884
HaeIII GGCC 5 cut(s) 87, 275, 716, 1328, 1894
HapII CCGG 1 cut(s) 1663
HhaI GCGC 2 cut(s) 867, 883
Hin6I GCGC 2 cut(s) 865, 881
HinP1I GCGC 2 cut(s) 865, 881
HincII GTYRAC 2 cut(s) 294, 2259
HindII GTYRAC 2 cut(s) 294, 2259
HindIII AAGCTT 3 cut(s) 2133, 2195, 3431
HpaII CCGG 1 cut(s) 1663
HphI GGTGA 7 cut(s) 435, 961, 1234, 1469, 2677, 2912, 2966
Hpy188III TCNNGA 9 cut(s) 539, 748, 1691, 1987, 2044, 2241, 2604, 3002, 3157
HpyAV CCTTC 5 cut(s) 1437, 1474, 3050, 3405, 3478
HpyCH4IV ACGT 2 cut(s) 401, 3145
HpyF10VI GCNNNNNNNGC 9 cut(s) 28, 515, 845, 927, 968, 1100, 1120, 3146, 3374
HpySE526I ACGT 2 cut(s) 401, 3145
HspAI GCGC 2 cut(s) 865, 881
Ksp22I TGATCA 1 cut(s) 186
LguI GCTCTTC 2 cut(s) 1093, 3113
LmnI GCTCC 3 cut(s) 1120, 1146, 2624
MaeI CTAG 6 cut(s) 123, 1496, 2241, 2687, 2775, 3011
MaeII ACGT 2 cut(s) 401, 3145
MaeIII GTNAC 7 cut(s) 77, 752, 1729, 2311, 2665, 2714, 2745
MfeI CAATTG 4 cut(s) 813, 2234, 3239, 3369
MflI RGATCY 4 cut(s) 366, 1194, 2025, 2071
MhlI GDGCHC 1 cut(s) 1450
MlsI TGGCCA 2 cut(s) 716, 1894
MluNI TGGCCA 2 cut(s) 716, 1894
MlyI GAGTC 5 cut(s) 3, 479, 760, 1373, 3169
MmeI TCCRAC 3 cut(s) 1420, 3255, 3544
Mox20I TGGCCA 2 cut(s) 716, 1894
Mph1103I ATGCAT 2 cut(s) 1803, 3096
MroXI GAANNNNTTC 2 cut(s) 1354, 3249
MscI TGGCCA 2 cut(s) 716, 1894
MslI CAYNNNNRTG 2 cut(s) 1552, 2723
Msp20I TGGCCA 2 cut(s) 716, 1894
MspA1I CMGCKG 3 cut(s) 231, 303, 2300
MspCI CTTAAG 3 cut(s) 703, 2189, 3164
MspI CCGG 1 cut(s) 1663
MspR9I CCNGG 2 cut(s) 1466, 2882
MunI CAATTG 4 cut(s) 813, 2234, 3239, 3369
Mva1269I GAATGC 2 cut(s) 1547, 2014
MvaI CCWGG 2 cut(s) 1466, 2882
MvnI CGCG 1 cut(s) 1902
MwoI GCNNNNNNNGC 9 cut(s) 28, 515, 845, 927, 968, 1100, 1120, 3146, 3374
NcoI CCATGG 2 cut(s) 555, 1272
NdeI CATATG 1 cut(s) 841
NlaIV GGNNCC 4 cut(s) 56, 1196, 2409, 3105
NmuCI GTSAC 3 cut(s) 752, 2665, 2714
NsbI TGCGCA 1 cut(s) 866
NsiI ATGCAT 2 cut(s) 1803, 3096
NspI RCATGY 3 cut(s) 200, 959, 1224
NspV TTCGAA 1 cut(s) 3462
PaeI GCATGC 1 cut(s) 200
PceI AGGCCT 1 cut(s) 87
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 2 cut(s) 1093, 3113
PcsI WCGNNNNNNNCGW 1 cut(s) 1641
PctI GAATGC 2 cut(s) 1547, 2014
PdmI GAANNNNTTC 2 cut(s) 1354, 3249
PflFI GACNNNGTC 1 cut(s) 3515
PflMI CCANNNNNTGG 1 cut(s) 922
Ple19I CGATCG 1 cut(s) 1674
PleI GAGTC 5 cut(s) 3, 478, 759, 1372, 3168
PmaCI CACGTG 1 cut(s) 3146
PmlI CACGTG 1 cut(s) 3146
PpsI GAGTC 5 cut(s) 3, 478, 759, 1372, 3168
Ppu21I YACGTR 1 cut(s) 3146
PscI ACATGT 1 cut(s) 955
PshBI ATTAAT 2 cut(s) 17, 2162
PsiI TTATAA 1 cut(s) 1148
Psp1406I AACGTT 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 1464, 2880
PspCI CACGTG 1 cut(s) 3146
PspFI CCCAGC 3 cut(s) 126, 231, 1520
PspGI CCWGG 2 cut(s) 1464, 2880
PspN4I GGNNCC 4 cut(s) 56, 1196, 2409, 3105
PspPI GGNCC 6 cut(s) 1326, 1462, 2408, 2831, 3104, 3134
PstNI CAGNNNCTG 1 cut(s) 2050
PsuI RGATCY 4 cut(s) 366, 1194, 2025, 2071
PsyI GACNNNGTC 1 cut(s) 3515
PvuI CGATCG 1 cut(s) 1674
PvuII CAGCTG 1 cut(s) 303
RsaI GTAC 8 cut(s) 337, 985, 1048, 1667, 1745, 2372, 2638, 2985
RsaNI GTAC 8 cut(s) 336, 984, 1047, 1666, 1744, 2371, 2637, 2984
RseI CAYNNNNRTG 2 cut(s) 1552, 2723
SapI GCTCTTC 2 cut(s) 1093, 3113
Sau96I GGNCC 6 cut(s) 1326, 1462, 2408, 2831, 3104, 3134
ScaI AGTACT 1 cut(s) 1745
SchI GAGTC 5 cut(s) 3, 479, 760, 1373, 3169
ScrFI CCNGG 2 cut(s) 1466, 2882
SduI GDGCHC 1 cut(s) 1450
SfcI CTRYAG 2 cut(s) 38, 159
SfuI TTCGAA 1 cut(s) 3462
SinI GGWCC 5 cut(s) 1462, 2408, 2831, 3104, 3134
SmiMI CAYNNNNRTG 2 cut(s) 1552, 2723
SmlI CTYRAG 7 cut(s) 44, 214, 703, 2189, 2756, 2930, 3164
SmoI CTYRAG 7 cut(s) 44, 214, 703, 2189, 2756, 2930, 3164
SphI GCATGC 1 cut(s) 200
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 8 cut(s) 229, 790, 927, 1902, 2155, 2300, 2663, 2703
SspI AATATT 2 cut(s) 1831, 2428
SspMI CTAG 6 cut(s) 123, 1496, 2241, 2687, 2775, 3011
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 1464, 2880
StyI CCWWGG 3 cut(s) 555, 1272, 2226
TaiI ACGT 2 cut(s) 404, 3148
TaqI TCGA 8 cut(s) 376, 538, 610, 1505, 1644, 1690, 3063, 3462
TaqII GACCGA 1 cut(s) 2904
TatI WGTACW 3 cut(s) 1743, 2370, 2983
TauI GCSGC 3 cut(s) 231, 930, 2157
TscAI CASTG 8 cut(s) 763, 1740, 1927, 2089, 2841, 3120, 3233, 3300
TseFI GTSAC 3 cut(s) 752, 2665, 2714
Tsp45I GTSAC 3 cut(s) 752, 2665, 2714
TspGWI ACGGA 2 cut(s) 96, 2083
TspRI CASTG 8 cut(s) 763, 1740, 1927, 2089, 2841, 3120, 3233, 3300
Tth111I GACNNNGTC 1 cut(s) 3515
Van91I CCANNNNNTGG 1 cut(s) 922
Vha464I CTTAAG 3 cut(s) 703, 2189, 3164
VpaK11BI GGWCC 5 cut(s) 1462, 2408, 2831, 3104, 3134
VspI ATTAAT 2 cut(s) 17, 2162
XagI CCTNNNNNAGG 1 cut(s) 455
XbaI TCTAGA 1 cut(s) 2240
XceI RCATGY 3 cut(s) 200, 959, 1224
XmiI GTMKAC 1 cut(s) 1417
XmnI GAANNNNTTC 2 cut(s) 1354, 3249
XspI CTAG 6 cut(s) 123, 1496, 2241, 2687, 2775, 3011
ZrmI AGTACT 1 cut(s) 1745
Zsp2I ATGCAT 2 cut(s) 1803, 3096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.