Rw5G048290

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000379
Physical Location & Seq
Reverse (-)
83895475 .. 83899996
4522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048290.1

Sequence Viewer

Length: 3420 bp
ATGAAGCGGGGTCCAAGTCCAATACGGAAGGATGATAATGATCCAGCATCTCGTCCTTCATCAGCAGCTGAAGCTTCATCATCATCACCTCCTCCTCCTGAGCAGAAGGATCATCCAGCATCTCTTCCTTCATCAGCAGCTGAAGTATCATCATCATCATCACCACCACCTCCTCCTCCTCCTCCTGGGCAGAAGTATGATGTGTTTTTGAGTTTCAGGGGTGCTGACACTCGCAAGGGTATCACATCTGATCTATACCATCGACTGCAGAGGAGGGGAATTAAAACATTCATGGATAATCCAGATCTTGAAGTTGGGGATGCTCTTTCTGACACTCTCTTAGCTGCAATTAAAGAATCAAGTTTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATGCATGGAAGACGACAGTAGAATCCTGCCACTTTTTTATAATGTTGATCCTACTGATGTGCGATTTCAGAAGAGGAGTTTCGAAGATGCTTTCACTAAGCATGTAAAATCTGGGCGACACAAATCAGAGAAGGTGGAGGAGTGGAAAGCTGGTTTATACAAAGTGGCCAATATCTCTGGGTGGAATACAAATGATCATAAGACTCACCAAGAACTGATCGACTCCATTGTGGAATCTGTCTACCGTAAAGTCTTACCTGCTGCAGTTGTGTTCATGGGAGAGTTCGAAGAGTTTCAAGCAACAAGACAAGCCATGGATGAGGTTACGAAGGCCCTAAAAGACGATGAAGTCAATGCCGTTGGGGTGTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATGTCGCTGCACAAGTCCGCAAAAATGGGATTTTTCATCATGTAATTATGGCTGTCGTTTCACAAAATCCTGATTTCGAAAAACTTCAAGATGCATTGGCAGAGCAGTTGGGCTTCAAATTGTGGGAGGAGACAGAAACTGGAAGAATTGCTAGATTGAATAAGGAGATAATGAGAACAGAAAAACTCCTTATAATCTTGGACGATGTTTGGGGGAGAACAGAGTTATCAAAGATAGGGATTCCCAGCTACAAGGAACTTCAAAAGTGCAAGTCCAAAGTCCTACTCACCACAAGGAGATTGAATGTATGCCTTACCATGGAGAGCCAAGCAAAAATTAAACTCAGTATCTTATCAGAAGAAGATGGTTGGAACTTGTTTGTGAACAAGGCACGAAGGTCATTTGAATCGACCACTTTTGAGGGTGTAGCGAGGACGGTAGCTGGAGAATGCAAAGGTCTACCCATTGCATTGATAGCAGTTGCAAGGGCACTTGGGGATAAACATCTGGAGGAATGGCAGACAGCAGCTCAACAATTAGAGAAGTCGCAACTTGCCAACCCTGATCATGACGAAGATGCTTTCAGATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAGTCATGCTTCTTACTGTGTTGCCTATATCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCCGGTACGCAATTGGGGAAGGATTGTTTCGAGATACTGACACAATATATGAAGCCAGAGGAATCGCACATTCAGTGGTCAAGCACCTGAAAGATTCTAGCTTGCTTTTGGAGGGTGAATTCAGTGGACACACTGGTGAATGTGTAAGGATGCATGATGTCGTCCGGGATACAGCCATGAGAATTGCAAAATGTGAAGATGGGCATGGGTTTTTGGTGAAAGCTGGATGTGGTTTAAATCATTGGTCGTGTCGATCACATGAAGGCTACTCTGCAATCTCACTAATGGAGAATAAAATTCGCCGTCTACCCGAAGAGTTGGTAAGTCCAAAACTTCAGATTTTATTACTACAACGGAATACTGATTTAAATGAGATCCCAGAGACCTTTTTCCAAAGTCCCAATGAATTAAGGCTCTTGGATCTTAGCAACACTAGTATTTCCTCTCTACCCCAATCTTTCAGTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTCTTGCGAGAAATTGATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGCATGAGGAAATATTCTGGGAGGGAATTGTCGAGAGAAATAGGAAATTTGACCAATCTAAGGATTTTGGAAGTCAGTGGCGCTTTAGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAGGAATTGTACATGCTGCACTGTGGATTTGAGTACTGGGGGTGTGAAATTGAGGGAAAAGGAGGAGAAACCAATATTGGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATTTTGCAGGTTTGCATATCTGATGCAAAATATATCCCTAAAAATGTTGAGGCCGAACCGAATTGGGTTTATTTTGATATACATATCGGCGGATTCATAAAATCTCTATACAATCGAATAGATCATAATTCAAGATTCTTGCATCTTGAAGGACCAATCATGAGTACCTTACCTGATTGGTTTATCAACGCGGTGACAGAGAAAACAGAGAAGCTAAAGTATGATCGCTGCGAAGGGATCAGTGACATTCTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTAAGTGGTCATTTTATTTCCTGTTTGTACTTGAAAGGGTTGATGAACACAACAAGACGAGTTCAAAATGTACCAGTGTTTGAGAATTTGGAAGAGTTGCACCTGATAGATCTGGTCCGCCTTAATGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTACTACATATTTGTTTTTGTTATATGTTGAAGAATGTATCAAAATTGGTACAGAGCCTACCAAATTTGGAGAAACTATATTTAAATTATATGGATGAATTGGAATATGTGTTTGGATGCGAAGGGTTAGAGCCGATACAATCAAAACTGAGAGAGATGCATTTGTTGGGTCCAAATTCAGTGAAAAGCATTTGTAGCGGTCCTGTCCCACGTGCAGTGTTCCAGAGTCTTAAAAGTTTGACCATTTACCGTTGCGAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCCAACTGTCTTGTTCAATTGGAAGACCTTTTAGTAGACAGGTGCCCTTTGTTGGAAAGATTAATTGAAGCAGTGAACAACGAGAAGATTACGGTTCTTTCAAAATTAAAGAATATGGTTTTGAAGAATCTTCCTGTGTTGAATGGTGCAAGTGCTACTGTTGATATTGAGTGTCCTTCATTGGAACGCTTGACTGTGGAGGAATGCCCCCAGTTTTCAACCTCTGCTTCCAACTACTTTGGCAGTAGGAACCCAGTCAAACTCAATGATCCACAACTACACCGCTTCCTATATGAAAGGCATCATATGCATGGGTCCTGCTGA

Protein Analysis

1139

Amino Acids

128.55

Weight (kDa)

5.92

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 65 - 230 2.2e-45 TIR domain
TIR_2 PF13676 68 - 175 1.3e-16 TIR domain
NB-ARC PF00931 249 - 413 5e-32 NB-ARC domain
LRR_8 PF13855 622 - 680 8.7e-10 Leucine rich repeat
LRR_14 PF23598 631 - 752 2.4e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 908 - 1047 1.3e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 988 - 1105 7.4e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 477, 1037
AasI GACNNNNNNGTC 1 cut(s) 785
Acc36I ACCTGC 2 cut(s) 703, 2335
AccB1I GGYRCC 1 cut(s) 3138
AccI GTMKAC 4 cut(s) 678, 1303, 1843, 3132
AccII CGCG 1 cut(s) 2528
AciI CCGC 7 cut(s) 7, 862, 2427, 2528, 2752, 3006, 3379
AclWI GGATC 7 cut(s) 35, 117, 479, 1906, 1965, 2582, 3359
AcoI YGGCCR 1 cut(s) 603
AcsI RAATTY 9 cut(s) 1655, 1833, 2088, 2140, 2329, 2337, 2719, 2872, 2983
AcuI CTGAAG 4 cut(s) 90, 162, 1856, 2096
AcvI CACGTG 1 cut(s) 3020
AcyI GRCGYC 1 cut(s) 819
AfaI GTAC 9 cut(s) 806, 1544, 2234, 2258, 2503, 2663, 2706, 2811, 2859
AfiI CCNNNNNNNGG 5 cut(s) 185, 1162, 2154, 2301, 3148
AflIII ACRYGT 1 cut(s) 844
AhdI GACNNNNNGTC 1 cut(s) 1474
AhlI ACTAGT 1 cut(s) 1970
AjnI CCWGG 2 cut(s) 184, 2785
AjuI GAANNNNNNNTTGG 4 cut(s) 2283, 2315, 2904, 2936
AleI CACNNNNGTG 1 cut(s) 1671
Alw26I GTCTC 4 cut(s) 968, 1913, 2006, 2796
AlwI GGATC 7 cut(s) 35, 117, 479, 1906, 1965, 2582, 3359
AlwNI CAGNNNCTG 3 cut(s) 68, 140, 983
AoxI GGCC 3 cut(s) 603, 768, 2388
ApoI RAATTY 9 cut(s) 1655, 1833, 2088, 2140, 2329, 2337, 2719, 2872, 2983
AseI ATTAAT 1 cut(s) 3158
Asp700I GAANNNNTTC 2 cut(s) 729, 927
AspLEI GCGC 1 cut(s) 2177
AspS9I GGNCC 7 cut(s) 11, 769, 2489, 2749, 2978, 3008, 3411
AsuC2I CCSGG 1 cut(s) 1703
AsuHPI GGTGA 9 cut(s) 78, 153, 635, 1123, 1664, 1685, 1765, 2542, 2786
AsuII TTCGAA 3 cut(s) 519, 723, 921
AvaII GGWCC 6 cut(s) 11, 2489, 2749, 2978, 3008, 3411
BaeGI GKGCMC 2 cut(s) 1336, 3143
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 3138
BbrPI CACGTG 1 cut(s) 3020
BbsI GAAGAC 4 cut(s) 453, 1515, 1536, 3126
BccI CCATC 4 cut(s) 267, 1202, 1730, 2200
BceAI ACGGC 3 cut(s) 779, 823, 1824
BciT130I CCWGG 2 cut(s) 186, 2787
BciVI GTATCC 1 cut(s) 1699
BclI TGATCA 2 cut(s) 631, 1408
BcnI CCSGG 1 cut(s) 1703
BcoDI GTCTC 4 cut(s) 968, 1913, 2006, 2796
BcuI ACTAGT 1 cut(s) 1970
BfaI CTAG 3 cut(s) 996, 1635, 1971
BfmI CTRYAG 3 cut(s) 266, 699, 2061
BfoI RGCGCY 1 cut(s) 2178
BfuAI ACCTGC 2 cut(s) 703, 2335
BfuI GTATCC 1 cut(s) 1699
BglII AGATCT 2 cut(s) 304, 2743
BmcAI AGTACT 1 cut(s) 2258
Bme1390I CCNGG 3 cut(s) 186, 1703, 2787
Bme18I GGWCC 6 cut(s) 11, 2489, 2749, 2978, 3008, 3411
BmeRI GACNNNNNGTC 1 cut(s) 1474
BmgT120I GGNCC 7 cut(s) 11, 769, 2489, 2749, 2978, 3008, 3411
BmiI GGNNCC 5 cut(s) 12, 2979, 3140, 3347, 3412
BmrFI CCNGG 3 cut(s) 186, 1703, 2787
BmrI ACTGGG 3 cut(s) 2269, 3301, 3344
BmuI ACTGGG 3 cut(s) 2269, 3301, 3344
BpiI GAAGAC 4 cut(s) 453, 1515, 1536, 3126
BpmI CTGGAG 2 cut(s) 1308, 1373
Bpu10I CCTNAGC 1 cut(s) 99
Bpu14I TTCGAA 3 cut(s) 519, 723, 921
BpuEI CTTGAG 2 cut(s) 2606, 2789
BpuMI CCSGG 1 cut(s) 1703
BsaAI YACGTR 1 cut(s) 3020
BsaBI GATNNNNATC 2 cut(s) 39, 1347
BsaHI GRCGYC 1 cut(s) 819
BsaI GGTCTC 2 cut(s) 1913, 2796
BsaJI CCNNGG 6 cut(s) 185, 750, 834, 1161, 2602, 2786
BsaWI WCCGGW 1 cut(s) 1539
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 1162, 2154, 2301, 3148
Bse1I ACTGG 6 cut(s) 988, 1675, 2264, 2708, 3307, 3350
Bse3DI GCAATG 1 cut(s) 1308
Bse8I GATNNNNATC 2 cut(s) 39, 1347
BseBI CCWGG 2 cut(s) 186, 2787
BseDI CCNNGG 6 cut(s) 185, 750, 834, 1161, 2602, 2786
BseGI GGATG 9 cut(s) 37, 112, 325, 760, 1518, 1692, 1769, 2908, 2930
BseJI GATNNNNATC 2 cut(s) 39, 1347
BseLI CCNNNNNNNGG 5 cut(s) 185, 1162, 2154, 2301, 3148
BseMI GCAATG 1 cut(s) 1308
BseMII CTCAG 3 cut(s) 90, 1201, 2948
BseNI ACTGG 6 cut(s) 988, 1675, 2264, 2708, 3307, 3350
BseSI GKGCMC 2 cut(s) 1336, 3143
BseYI CCCAGC 1 cut(s) 1088
BsgI GTGCAG 3 cut(s) 837, 2225, 3042
Bsh1236I CGCG 1 cut(s) 2528
BshFI GGCC 3 cut(s) 605, 770, 2390
BshNI GGYRCC 1 cut(s) 3138
BsiSI CCGG 2 cut(s) 1540, 1702
BslFI GGGAC 2 cut(s) 1920, 2999
BslI CCNNNNNNNGG 5 cut(s) 185, 1162, 2154, 2301, 3148
BsmAI GTCTC 4 cut(s) 968, 1913, 2006, 2796
BsmFI GGGAC 2 cut(s) 1920, 2999
BsmI GAATGC 2 cut(s) 1298, 3305
BsnI GGCC 3 cut(s) 605, 770, 2390
Bso31I GGTCTC 2 cut(s) 1913, 2796
Bsp119I TTCGAA 3 cut(s) 519, 723, 921
Bsp1286I GDGCHC 2 cut(s) 1336, 3143
Bsp1407I TGTACA 1 cut(s) 2232
Bsp19I CCATGG 4 cut(s) 750, 834, 1161, 2602
BspACI CCGC 7 cut(s) 7, 862, 2427, 2528, 2752, 3006, 3379
BspANI GGCC 3 cut(s) 605, 770, 2390
BspCNI CTCAG 3 cut(s) 91, 1200, 2949
BspFNI CGCG 1 cut(s) 2528
BspHI TCATGA 2 cut(s) 1411, 2496
BspLI GGNNCC 5 cut(s) 12, 2979, 3140, 3347, 3412
BspMAI CTGCAG 2 cut(s) 270, 703
BspMI ACCTGC 2 cut(s) 703, 2335
BspPI GGATC 7 cut(s) 35, 117, 479, 1906, 1965, 2582, 3359
BspT104I TTCGAA 3 cut(s) 519, 723, 921
BspT107I GGYRCC 1 cut(s) 3138
BspTNI GGTCTC 2 cut(s) 1913, 2796
BsrDI GCAATG 1 cut(s) 1308
BsrGI TGTACA 1 cut(s) 2232
BsrI ACTGG 6 cut(s) 988, 1675, 2264, 2708, 3307, 3350
BssECI CCNNGG 6 cut(s) 185, 750, 834, 1161, 2602, 2786
BssNI GRCGYC 1 cut(s) 819
BssT1I CCWWGG 4 cut(s) 750, 834, 1161, 2602
Bst2UI CCWGG 2 cut(s) 186, 2787
Bst6I CTCTTC 5 cut(s) 129, 503, 720, 1845, 2721
BstACI GRCGYC 1 cut(s) 819
BstAPI GCANNNNNTGC 1 cut(s) 3403
BstAUI TGTACA 1 cut(s) 2232
BstBAI YACGTR 1 cut(s) 3020
BstBI TTCGAA 3 cut(s) 519, 723, 921
BstC8I GCNNGC 2 cut(s) 1640, 3065
BstDEI CTNAG 9 cut(s) 99, 340, 422, 534, 1187, 1961, 2093, 2153, 2957
BstDSI CCRYGG 4 cut(s) 750, 834, 1161, 2602
BstF5I GGATG 9 cut(s) 37, 112, 325, 760, 1518, 1692, 1769, 2908, 2930
BstFNI CGCG 1 cut(s) 2528
BstH2I RGCGCY 1 cut(s) 2178
BstHHI GCGC 1 cut(s) 2177
BstMAI GTCTC 4 cut(s) 968, 1913, 2006, 2796
BstMWI GCNNNNNNNGC 5 cut(s) 71, 816, 1319, 3003, 3403
BstNI CCWGG 2 cut(s) 186, 2787
BstNSI RCATGY 3 cut(s) 542, 848, 2239
BstSCI CCNGG 3 cut(s) 184, 1701, 2785
BstSFI CTRYAG 3 cut(s) 266, 699, 2061
BstSLI GKGCMC 2 cut(s) 1336, 3143
BstUI CGCG 1 cut(s) 2528
BstV2I GAAGAC 4 cut(s) 453, 1515, 1536, 3126
BstX2I RGATCY 4 cut(s) 304, 1911, 1957, 2743
BstYI RGATCY 4 cut(s) 304, 1911, 1957, 2743
BsuI GTATCC 1 cut(s) 1699
BsuRI GGCC 3 cut(s) 605, 770, 2390
BtgI CCRYGG 4 cut(s) 750, 834, 1161, 2602
BtsCI GGATG 9 cut(s) 37, 112, 325, 760, 1518, 1692, 1769, 2908, 2930
BtsI GCAGTG 2 cut(s) 3030, 3174
BveI ACCTGC 2 cut(s) 703, 2335
Cac8I GCNNGC 2 cut(s) 1640, 3065
CaiI CAGNNNCTG 3 cut(s) 68, 140, 983
CciI TCATGA 2 cut(s) 1411, 2496
CfoI GCGC 1 cut(s) 2177
Cfr13I GGNCC 7 cut(s) 11, 769, 2489, 2749, 2978, 3008, 3411
CseI GACGC 1 cut(s) 808
Csp6I GTAC 9 cut(s) 805, 1543, 2233, 2257, 2502, 2662, 2705, 2810, 2858
CviQI GTAC 9 cut(s) 805, 1543, 2233, 2257, 2502, 2662, 2705, 2810, 2858
DdeI CTNAG 9 cut(s) 99, 340, 422, 534, 1187, 1961, 2093, 2153, 2957
DraI TTTAAA 3 cut(s) 1773, 1905, 2892
DrdI GACNNNNNNGTC 1 cut(s) 785
DriI GACNNNNNGTC 1 cut(s) 1474
DseDI GACNNNNNNGTC 1 cut(s) 785
EaeI YGGCCR 1 cut(s) 603
Eam1104I CTCTTC 5 cut(s) 129, 503, 720, 1845, 2721
Eam1105I GACNNNNNGTC 1 cut(s) 1474
EarI CTCTTC 5 cut(s) 129, 503, 720, 1845, 2721
EciI GGCGGA 2 cut(s) 2442, 2741
Eco130I CCWWGG 4 cut(s) 750, 834, 1161, 2602
Eco31I GGTCTC 2 cut(s) 1913, 2796
Eco32I GATATC 1 cut(s) 2205
Eco47I GGWCC 6 cut(s) 11, 2489, 2749, 2978, 3008, 3411
Eco57I CTGAAG 4 cut(s) 90, 162, 1856, 2096
Eco72I CACGTG 1 cut(s) 3020
EcoO109I RGGNCCY 2 cut(s) 769, 3411
EcoRI GAATTC 1 cut(s) 1655
EcoRII CCWGG 2 cut(s) 184, 2785
EcoRV GATATC 1 cut(s) 2205
EcoT14I CCWWGG 4 cut(s) 750, 834, 1161, 2602
EcoT22I ATGCAT 5 cut(s) 443, 940, 1692, 2970, 3408
ErhI CCWWGG 4 cut(s) 750, 834, 1161, 2602
FalI AAGNNNNNCTT 2 cut(s) 1466, 1498
FaqI GGGAC 2 cut(s) 1920, 2999
FauNDI CATATG 1 cut(s) 3402
FbaI TGATCA 2 cut(s) 631, 1408
FblI GTMKAC 4 cut(s) 678, 1303, 1843, 3132
FokI GGATG 9 cut(s) 44, 99, 332, 767, 1505, 1699, 1776, 2915, 2937
FspBI CTAG 3 cut(s) 996, 1635, 1971
GlaI GCGC 1 cut(s) 2176
GsaI CCCAGC 1 cut(s) 1092
GsuI CTGGAG 2 cut(s) 1308, 1373
HaeII RGCGCY 1 cut(s) 2178
HaeIII GGCC 3 cut(s) 605, 770, 2390
HapII CCGG 2 cut(s) 1540, 1702
HgaI GACGC 1 cut(s) 808
HhaI GCGC 1 cut(s) 2177
Hin1I GRCGYC 1 cut(s) 819
Hin6I GCGC 1 cut(s) 2175
HinP1I GCGC 1 cut(s) 2175
HindIII AAGCTT 3 cut(s) 72, 2019, 2081
HpaII CCGG 2 cut(s) 1540, 1702
HphI GGTGA 9 cut(s) 78, 153, 635, 1123, 1664, 1685, 1765, 2542, 2786
Hpy166II GTNNAC 8 cut(s) 679, 805, 1228, 1304, 1664, 1844, 3133, 3172
Hpy188I TCNGA 9 cut(s) 250, 331, 507, 565, 1201, 1430, 1875, 2359, 3091
Hpy8I GTNNAC 8 cut(s) 679, 805, 1228, 1304, 1664, 1844, 3133, 3172
Hpy99I CGWCG 1 cut(s) 824
HpyCH4IV ACGT 1 cut(s) 3019
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 816, 1319, 3003, 3403
HpyF3I CTNAG 9 cut(s) 99, 340, 422, 534, 1187, 1961, 2093, 2153, 2957
HpySE526I ACGT 1 cut(s) 3019
Hsp92I GRCGYC 1 cut(s) 819
HspAI GCGC 1 cut(s) 2175
Ksp22I TGATCA 2 cut(s) 631, 1408
MaeI CTAG 3 cut(s) 996, 1635, 1971
MaeII ACGT 1 cut(s) 3019
MaeIII GTNAC 6 cut(s) 760, 2182, 2530, 2579, 2610, 2778
MfeI CAATTG 2 cut(s) 1548, 3113
MflI RGATCY 4 cut(s) 304, 1911, 1957, 2743
MhlI GDGCHC 2 cut(s) 1336, 3143
MlsI TGGCCA 1 cut(s) 605
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 3 cut(s) 634, 653, 3043
MmeI TCCRAC 3 cut(s) 1193, 3129, 3351
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 5 cut(s) 443, 940, 1692, 2970, 3408
MroXI GAANNNNTTC 2 cut(s) 729, 927
MscI TGGCCA 1 cut(s) 605
MslI CAYNNNNRTG 3 cut(s) 1671, 2588, 3405
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 2 cut(s) 68, 140
MspI CCGG 2 cut(s) 1540, 1702
MspR9I CCNGG 3 cut(s) 186, 1703, 2787
MunI CAATTG 2 cut(s) 1548, 3113
Mva1269I GAATGC 2 cut(s) 1298, 3305
MvaI CCWGG 2 cut(s) 186, 2787
MvnI CGCG 1 cut(s) 2528
MwoI GCNNNNNNNGC 5 cut(s) 71, 816, 1319, 3003, 3403
NciI CCSGG 1 cut(s) 1703
NcoI CCATGG 4 cut(s) 750, 834, 1161, 2602
NdeI CATATG 1 cut(s) 3402
NlaIV GGNNCC 5 cut(s) 12, 2979, 3140, 3347, 3412
NmuCI GTSAC 3 cut(s) 2182, 2530, 2579
NsiI ATGCAT 5 cut(s) 443, 940, 1692, 2970, 3408
NspI RCATGY 3 cut(s) 542, 848, 2239
NspV TTCGAA 3 cut(s) 519, 723, 921
OliI CACNNNNGTG 1 cut(s) 1671
PagI TCATGA 2 cut(s) 1411, 2496
PciI ACATGT 1 cut(s) 844
PctI GAATGC 2 cut(s) 1298, 3305
PdmI GAANNNNTTC 2 cut(s) 729, 927
PfoI TCCNGGA 1 cut(s) 1701
PleI GAGTC 3 cut(s) 634, 653, 3042
PmaCI CACGTG 1 cut(s) 3020
PmlI CACGTG 1 cut(s) 3020
PpsI GAGTC 3 cut(s) 634, 653, 3042
Ppu21I YACGTR 1 cut(s) 3020
PpuMI RGGWCCY 1 cut(s) 3411
PscI ACATGT 1 cut(s) 844
PshBI ATTAAT 1 cut(s) 3158
PsiI TTATAA 2 cut(s) 477, 1037
Psp5II RGGWCCY 1 cut(s) 3411
Psp6I CCWGG 2 cut(s) 184, 2785
PspCI CACGTG 1 cut(s) 3020
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 2 cut(s) 184, 2785
PspN4I GGNNCC 5 cut(s) 12, 2979, 3140, 3347, 3412
PspPI GGNCC 7 cut(s) 11, 769, 2489, 2749, 2978, 3008, 3411
PspPPI RGGWCCY 1 cut(s) 3411
PstI CTGCAG 2 cut(s) 270, 703
PstNI CAGNNNCTG 3 cut(s) 68, 140, 983
PsuI RGATCY 4 cut(s) 304, 1911, 1957, 2743
PvuII CAGCTG 2 cut(s) 68, 140
RsaI GTAC 9 cut(s) 806, 1544, 2234, 2258, 2503, 2663, 2706, 2811, 2859
RsaNI GTAC 9 cut(s) 805, 1543, 2233, 2257, 2502, 2662, 2705, 2810, 2858
RseI CAYNNNNRTG 3 cut(s) 1671, 2588, 3405
Sau96I GGNCC 7 cut(s) 11, 769, 2489, 2749, 2978, 3008, 3411
ScaI AGTACT 1 cut(s) 2258
SchI GAGTC 3 cut(s) 634, 653, 3043
ScrFI CCNGG 3 cut(s) 186, 1703, 2787
SduI GDGCHC 2 cut(s) 1336, 3143
SfcI CTRYAG 3 cut(s) 266, 699, 2061
SfuI TTCGAA 3 cut(s) 519, 723, 921
SinI GGWCC 6 cut(s) 11, 2489, 2749, 2978, 3008, 3411
SmiI ATTTAAAT 2 cut(s) 1905, 2892
SmiMI CAYNNNNRTG 3 cut(s) 1671, 2588, 3405
SmlI CTYRAG 2 cut(s) 2621, 2804
SmoI CTYRAG 2 cut(s) 2621, 2804
SpeI ACTAGT 1 cut(s) 1970
SsiI CCGC 7 cut(s) 7, 862, 2427, 2528, 2752, 3006, 3379
SspI AATATT 2 cut(s) 2108, 2299
SspMI CTAG 3 cut(s) 996, 1635, 1971
StyD4I CCNGG 3 cut(s) 184, 1701, 2785
StyI CCWWGG 4 cut(s) 750, 834, 1161, 2602
SwaI ATTTAAAT 2 cut(s) 1905, 2892
TaiI ACGT 1 cut(s) 3022
TatI WGTACW 3 cut(s) 2232, 2256, 2661
TseFI GTSAC 3 cut(s) 2182, 2530, 2579
Tsp45I GTSAC 3 cut(s) 2182, 2530, 2579
TspGWI ACGGA 2 cut(s) 40, 1906
VpaK11BI GGWCC 6 cut(s) 11, 2489, 2749, 2978, 3008, 3411
VspI ATTAAT 1 cut(s) 3158
XapI RAATTY 9 cut(s) 1655, 1833, 2088, 2140, 2329, 2337, 2719, 2872, 2983
XceI RCATGY 3 cut(s) 542, 848, 2239
XmiI GTMKAC 4 cut(s) 678, 1303, 1843, 3132
XmnI GAANNNNTTC 2 cut(s) 729, 927
XspI CTAG 3 cut(s) 996, 1635, 1971
ZrmI AGTACT 1 cut(s) 2258
Zsp2I ATGCAT 5 cut(s) 443, 940, 1692, 2970, 3408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.