FvH4_3g45730

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37929463 .. 37937597
8135 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45730.t6

Sequence Viewer

Length: 3357 bp
ATGGCCTTCTCCTCTTCATCATCGTCAACTCGCAGCTGGAAGTATGATGTCTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAAGGGATTGCAAACGAGATCTGCAATCGACTTCGTAGAAGTGGCATTAAAACGTTCATGGATGAGCATGGACTTCAAGTAGGGGATGTTATTTCTCCCACACTCATCCAGGCAATTAAAGAGTCAAGGTTTGCAATCGTAATTCTCTCAAAAAATTACGCTTCTTCTGCTTGGTGTTTGGAGGAACTTCGAGAGATTTGTCAATCCATGGAAGACAACAGAATTTTGCCACTTTTTTTTGAAGTTGAACCTACTGATGTTCGATATCAGAAGAGGAGTTTCGAAGAAGCTTTCTCTAAGCACGATACCTCTGGGCGACATGAATCAGAGAAGGTGGAGCAATGGAGAGCTGCTCTAGAAAAAGTGGCCAATATCTCAGGGTGGAATACAAATGATTTCAAGACTCACGAAGAACTTGTTGACAACATTGTGGAATCTCTCCGCAGTAAAGTTACACCTGATGCAATTGAATTCACAGATGATTTCCAAGCATTTGGAGCAACAAAAGAAGCGACAGACAAGGTTATGAAGGCGCTAACAGATTACAAGATTACTGCCGTTGGTGTCCACGGCATGGGCGGCGTTGGGAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAGAAATGGTACTTTTAACCATTGGATTATGGCTGTTGTATCCCAAAACCTTGACTTGAAAAAAATTCAAGGTACATTGGCAGATTTGTTGGGCTTTAAATTCGTGGAGGAGACAGAAGCTGGAAGAGCCGTTAGGTTGCATAAGGAGATCATGAGAAAAGAAAAACTCCTGATAATTGTGGACGATGTCTGGGAGAGAACAGAGTTGTCAAAGATAGGGATTCCCCCCTACAATGAGCTTCAAAAGTGTAAGTCCAAAGTGCTGCTCACCACGAGGAGATTGAATGTCTGTAATGACATGAAGTGCCATGAAAAGATTGCCCTCAAGCTTCTCTCGAAAAAAGATTCTTGGAACTTGTTTCTGAGAAATGCAGGAACAATTTCTTTTGAGTCCACCTCGTTTGAGGAAGTGGCAAGGAAGGTAGCTGGAGAGTGTAAGGGTCTACCGATTGCATTGGTAGCTGTTGCAAGAGCACTCGGAGATGAGGACCAGGAGGAATGGAAGAAGGCAGCTGAACGACTAGAGCAGTCGCAATATGTCAATCCTAACCATGAGGAATGTGAGGAAAATGCTTTCAGATATATACGATTAAGCTATGATTACTTGAAAAATGAAGACCTCAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCATGACATCAAAATAGAAGACTTGTTCCGGTACGTGATCGGAAAAAGATTGTTTCGAGATGCCAAAACAATGGAAGTAGCCAGAGGAAGATTAGTTACAGTGGTCAAGTACCTGAAAAATTCTAGCTTGCTTTTGGATAGCCAGAAAAAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCCAAATCTGAAGATTGTCATCGGTTTTTGGTAGAAGCTGGTCGTGGTTTAAAGGATTGGGCGCCACGCGGATTACATGAAGGCTGCACTGCAATTTCACTGATGAACAACAAAATTCGCAAGCTACCTGAAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTTTTACTAAACGGGAATGCTGAACTAAGGAAGATCCCTGAAAAGTTTATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGCAACACTGGTATTTCCGTATTACCCCAATCATTTAGTCTCCTCACCAACCTCCAAGCTTTGTATTTAGATGATTGCGACAAATTGATTGACATTTCTGTTGTGGGGAAACTTCACAAGCTTGAAATTCTTAGTATGAGAGAACGTCCAGGGGGGGAATTGTCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGACGTCAATCGCTCTTTGACATTATTTGATGGGAGAGGAATAAAGGAACAATCTAAGGTTACAATTCCATCTAAAGTGATATCAAAGTTGCAAGAATTAGAAGAATTGTACTGTGGATTTGAGGAGGACGGGTCGTGTGTAGCTGAGGGACCAACAGAAAGAACCAATATTATTAGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCTTATCCGATGAAGGTTTCAGCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACTTTTGTATAAGTGTCTTTGGCAGATGCTGGTACTCTATCCCTGCTTCATACGTACAAGGAGATCGTAATTCAAGATCCTTGTTTCTTCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATTACCGCTGCGGAGGGATGAGTGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTTACTGGGCAAGAAGACGAGTTGAAAGAGTTGATGAACACAAAAAGACGAGTTGAAACAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGAAATTAGTGTTCGACCTAGAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTGTTTCATGTGTATTATGGTTGGATTTTGAGGAGTGTATCAAAATTTGTACAAAGACTACCAAATCTGGAGAAACTAGATTTAAACTCTATGCTCAAATTGGAATATGTGTTCAGATGCGAAGGGTTCGAGCCAGAACAATCAAAACTGAGAGAGATGCATTTGTTGTGGCAAGAAGCACTAAGAAACATATGTAATGGTCCCGCTCCACATGCAATGTTCCAGAGTCTTCAGATTTTGACGATTTACCGTTGCCAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTGAGTGTCTTGTTCAATTGGAAGACCTTCTTGTAGAGGATTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGGTTCTACCAAAACTGAAGAACTTGGTTTTGAAGAATCTTCCTTTGTTGTATGGTGCAATTGCTGCCGTTGATATTGAGTGTCCTTCATTGGAACACTTGATGGTGGTGGGTTGCCCCAAGCTTTTATTTTCAACCTCTTCCGATCTCTTCGAAGCCTTCAAAAGCAGGAACCAATTTTCATTTTCAGCCTCCACTTCTCACCCAGTCCAACTCCATGATCCACAACTATACAAAACTCTACGTGACAGAACATCTGGCTTCATATTGCGAAGAGTCTAG

Protein Analysis

1119

Amino Acids

127.45

Weight (kDa)

6.29

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 14 - 85 1.2e-08 TIR domain
NB-ARC PF00931 103 - 266 2.8e-29 NB-ARC domain
LRR_8 PF13855 478 - 536 2.5e-09 Leucine rich repeat
LRR_14 PF23598 488 - 616 1.2e-15 Leucine-rich repeat region
LRR_RPS2 PF23247 778 - 917 3.9e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 858 - 973 3.5e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2019
Acc36I ACCTGC 1 cut(s) 2221
AccB1I GGYRCC 1 cut(s) 1627
AccB7I CCANNNNNTGG 1 cut(s) 655
AccBSI CCGCTC 1 cut(s) 2906
AccI GTMKAC 1 cut(s) 1150
AccII CGCG 1 cut(s) 1635
AciI CCGC 7 cut(s) 523, 660, 1635, 2429, 2464, 2469, 2904
AclI AACGTT 1 cut(s) 134
AclWI GGATC 4 cut(s) 1756, 1815, 2364, 3290
AcoI YGGCCR 1 cut(s) 447
AcuI CTGAAG 4 cut(s) 1596, 1716, 2915, 3113
AcyI GRCGYC 2 cut(s) 1628, 2016
AfaI GTAC 9 cut(s) 718, 781, 1400, 1478, 2123, 2327, 2349, 2404, 2751
AfiI CCNNNNNNNGG 3 cut(s) 655, 2004, 3040
AflIII ACRYGT 1 cut(s) 688
AhdI GACNNNNNGTC 1 cut(s) 1330
AjnI CCWGG 4 cut(s) 189, 1197, 1960, 2677
AloI GAACNNNNNNTCC 2 cut(s) 2795, 2827
Alw21I GWGCWC 1 cut(s) 1183
Alw26I GTCTC 4 cut(s) 679, 812, 1856, 2688
AlwI GGATC 4 cut(s) 1756, 1815, 2364, 3290
AlwNI CAGNNNCTG 5 cut(s) 827, 1786, 2322, 2540, 2962
AoxI GGCC 2 cut(s) 3, 447
Asp700I GAANNNNTTC 2 cut(s) 1087, 3015
AspLEI GCGC 2 cut(s) 616, 1630
AspS9I GGNCC 5 cut(s) 68, 1195, 2162, 2597, 2900
AsuC2I CCSGG 1 cut(s) 1547
AsuHPI GGTGA 6 cut(s) 694, 967, 1849, 2443, 2678, 3269
AsuII TTCGAA 2 cut(s) 363, 3228
AvaII GGWCC 5 cut(s) 68, 1195, 2162, 2597, 2900
BalI TGGCCA 1 cut(s) 449
BanI GGYRCC 1 cut(s) 1627
BarI GAAGNNNNNNTAC 2 cut(s) 1447, 1479
BauI CACGAG 1 cut(s) 979
BbsI GAAGAC 9 cut(s) 300, 677, 1329, 1371, 1392, 2556, 2921, 3018, 3080
Bbv12I GWGCWC 1 cut(s) 1183
BbvCI CCTCAGC 1 cut(s) 2157
BccI CCATC 4 cut(s) 2036, 2089, 2405, 3172
BceAI ACGGC 4 cut(s) 623, 667, 821, 3128
BciT130I CCWGG 4 cut(s) 191, 1199, 1962, 2679
BciVI GTATCC 2 cut(s) 757, 1543
BcnI CCSGG 1 cut(s) 1547
BcoDI GTCTC 4 cut(s) 679, 812, 1856, 2688
BfaI CTAG 7 cut(s) 437, 1229, 1491, 2453, 2648, 2777, 3355
BfmI CTRYAG 1 cut(s) 706
BfoI RGCGCY 2 cut(s) 617, 1631
BfuAI ACCTGC 1 cut(s) 2221
BfuI GTATCC 2 cut(s) 757, 1543
BglII AGATCT 1 cut(s) 99
Bme1390I CCNGG 5 cut(s) 191, 1199, 1547, 1962, 2679
Bme18I GGWCC 5 cut(s) 68, 1195, 2162, 2597, 2900
BmeRI GACNNNNNGTC 1 cut(s) 1330
BmgT120I GGNCC 5 cut(s) 68, 1195, 2162, 2597, 2900
BmiI GGNNCC 5 cut(s) 69, 1629, 2163, 2902, 3248
BmrFI CCNGG 5 cut(s) 191, 1199, 1547, 1962, 2679
BmrI ACTGGG 2 cut(s) 2550, 3275
BmsI GCATC 9 cut(s) 532, 1417, 1521, 2309, 2536, 2635, 2807, 2847, 2987
BmuI ACTGGG 2 cut(s) 2550, 3275
BpiI GAAGAC 9 cut(s) 300, 677, 1329, 1371, 1392, 2556, 2921, 3018, 3080
BplI GAGNNNNNCTC 4 cut(s) 418, 450, 1088, 1120
BpmI CTGGAG 3 cut(s) 1155, 1706, 2789
Bpu10I CCTNAGC 1 cut(s) 2157
Bpu14I TTCGAA 2 cut(s) 363, 3228
BpuEI CTTGAG 4 cut(s) 1016, 1313, 2507, 2681
BpuMI CCSGG 1 cut(s) 1547
BsaAI YACGTR 3 cut(s) 1402, 2347, 3320
BsaBI GATNNNNATC 1 cut(s) 1584
BsaHI GRCGYC 2 cut(s) 1628, 2016
BsaI GGTCTC 1 cut(s) 2688
BsaJI CCNNGG 5 cut(s) 288, 649, 1961, 2503, 2678
BsaWI WCCGGW 1 cut(s) 1395
BsaXI ACNNNNNCTCC 2 cut(s) 2129, 2159
Bsc4I CCNNNNNNNGG 3 cut(s) 655, 2004, 3040
Bse1I ACTGG 4 cut(s) 1825, 2545, 2600, 3281
Bse3DI GCAATG 2 cut(s) 428, 2922
Bse8I GATNNNNATC 1 cut(s) 1584
BseBI CCWGG 4 cut(s) 191, 1199, 1962, 2679
BseDI CCNNGG 5 cut(s) 288, 649, 1961, 2503, 2678
BseGI GGATG 6 cut(s) 148, 172, 186, 1526, 1542, 2481
BseJI GATNNNNATC 1 cut(s) 1584
BseLI CCNNNNNNNGG 3 cut(s) 655, 2004, 3040
BseMI GCAATG 2 cut(s) 428, 2922
BseMII CTCAG 5 cut(s) 471, 1061, 2148, 2840, 2982
BseNI ACTGG 4 cut(s) 1825, 2545, 2600, 3281
BseRI GAGGAG 7 cut(s) 370, 830, 997, 1844, 2150, 2666, 2746
BsgI GTGCAG 2 cut(s) 681, 1636
Bsh1236I CGCG 1 cut(s) 1635
BshFI GGCC 2 cut(s) 5, 449
BshNI GGYRCC 1 cut(s) 1627
BsiHKAI GWGCWC 1 cut(s) 1183
BsiSI CCGG 2 cut(s) 1396, 1546
BslFI GGGAC 2 cut(s) 2175, 2886
BslI CCNNNNNNNGG 3 cut(s) 655, 2004, 3040
BsmAI GTCTC 4 cut(s) 679, 812, 1856, 2688
BsmFI GGGAC 2 cut(s) 2175, 2886
BsmI GAATGC 1 cut(s) 1750
BsnI GGCC 2 cut(s) 5, 449
Bso31I GGTCTC 1 cut(s) 2688
Bsp119I TTCGAA 2 cut(s) 363, 3228
Bsp1286I GDGCHC 1 cut(s) 1183
Bsp1407I TGTACA 1 cut(s) 2749
Bsp143I GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
Bsp19I CCATGG 2 cut(s) 288, 2503
BspACI CCGC 7 cut(s) 523, 660, 1635, 2429, 2464, 2469, 2904
BspANI GGCC 2 cut(s) 5, 449
BspCNI CTCAG 5 cut(s) 470, 1062, 2149, 2841, 2983
BspFNI CGCG 1 cut(s) 1635
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 5 cut(s) 69, 1629, 2163, 2902, 3248
BspMAI CTGCAG 1 cut(s) 710
BspMI ACCTGC 1 cut(s) 2221
BspPI GGATC 4 cut(s) 1756, 1815, 2364, 3290
BspQI GCTCTTC 1 cut(s) 826
BspT104I TTCGAA 2 cut(s) 363, 3228
BspT107I GGYRCC 1 cut(s) 1627
BspTNI GGTCTC 1 cut(s) 2688
BsrBI CCGCTC 1 cut(s) 2906
BsrDI GCAATG 2 cut(s) 428, 2922
BsrGI TGTACA 1 cut(s) 2749
BsrI ACTGG 4 cut(s) 1825, 2545, 2600, 3281
BssECI CCNNGG 5 cut(s) 288, 649, 1961, 2503, 2678
BssMI GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
BssNI GRCGYC 2 cut(s) 1628, 2016
BssSI CACGAG 1 cut(s) 979
BssT1I CCWWGG 2 cut(s) 288, 2503
Bst2BI CACGAG 1 cut(s) 979
Bst2UI CCWGG 4 cut(s) 191, 1199, 1962, 2679
Bst4CI ACNGT 5 cut(s) 1468, 2126, 2536, 2951, 3079
Bst6I CTCTTC 8 cut(s) 19, 347, 826, 1695, 2613, 3220, 3230, 3343
BstACI GRCGYC 2 cut(s) 1628, 2016
BstAPI GCANNNNNTGC 1 cut(s) 3140
BstAUI TGTACA 1 cut(s) 2749
BstBAI YACGTR 3 cut(s) 1402, 2347, 3320
BstBI TTCGAA 2 cut(s) 363, 3228
BstC8I GCNNGC 4 cut(s) 706, 1496, 1688, 2957
BstDSI CCRYGG 3 cut(s) 288, 649, 2503
BstF5I GGATG 6 cut(s) 148, 172, 186, 1526, 1542, 2481
BstFNI CGCG 1 cut(s) 1635
BstH2I RGCGCY 2 cut(s) 617, 1631
BstHHI GCGC 2 cut(s) 616, 1630
BstKTI GATC 9 cut(s) 102, 858, 1407, 1764, 1810, 2359, 2372, 3223, 3298
BstMAI GTCTC 4 cut(s) 679, 812, 1856, 2688
BstMBI GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
BstMWI GCNNNNNNNGC 8 cut(s) 248, 578, 660, 701, 833, 1166, 2912, 3140
BstNI CCWGG 4 cut(s) 191, 1199, 1962, 2679
BstNSI RCATGY 2 cut(s) 692, 2915
BstSCI CCNGG 5 cut(s) 189, 1197, 1545, 1960, 2677
BstSFI CTRYAG 1 cut(s) 706
BstSNI TACGTA 1 cut(s) 2347
BstUI CGCG 1 cut(s) 1635
BstV2I GAAGAC 9 cut(s) 300, 677, 1329, 1371, 1392, 2556, 2921, 3018, 3080
BstX2I RGATCY 4 cut(s) 99, 1761, 1807, 2369
BstXI CCANNNNNNTGG 2 cut(s) 575, 1438
BstYI RGATCY 4 cut(s) 99, 1761, 1807, 2369
BsuI GTATCC 2 cut(s) 757, 1543
BsuRI GGCC 2 cut(s) 5, 449
BtgI CCRYGG 3 cut(s) 288, 649, 2503
BtsCI GGATG 6 cut(s) 148, 172, 186, 1526, 1542, 2481
BtsI GCAGTG 2 cut(s) 1653, 3066
BtsIMutI CAGTG 6 cut(s) 1473, 1653, 1664, 1818, 2607, 3066
BveI ACCTGC 1 cut(s) 2221
Cac8I GCNNGC 4 cut(s) 706, 1496, 1688, 2957
CaiI CAGNNNCTG 5 cut(s) 827, 1786, 2322, 2540, 2962
CciI TCATGA 1 cut(s) 858
CfoI GCGC 2 cut(s) 616, 1630
Cfr13I GGNCC 5 cut(s) 68, 1195, 2162, 2597, 2900
Csp6I GTAC 9 cut(s) 717, 780, 1399, 1477, 2122, 2326, 2348, 2403, 2750
CspCI CAANNNNNGTGG 2 cut(s) 1090, 1125
CviQI GTAC 9 cut(s) 717, 780, 1399, 1477, 2122, 2326, 2348, 2403, 2750
DinI GGCGCC 1 cut(s) 1629
DpnI GATC 9 cut(s) 101, 857, 1406, 1763, 1809, 2358, 2371, 3222, 3297
DpnII GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
DraI TTTAAA 3 cut(s) 805, 1617, 2784
DriI GACNNNNNGTC 1 cut(s) 1330
EaeI YGGCCR 1 cut(s) 447
Eam1104I CTCTTC 8 cut(s) 19, 347, 826, 1695, 2613, 3220, 3230, 3343
Eam1105I GACNNNNNGTC 1 cut(s) 1330
EarI CTCTTC 8 cut(s) 19, 347, 826, 1695, 2613, 3220, 3230, 3343
Eco105I TACGTA 1 cut(s) 2347
Eco130I CCWWGG 2 cut(s) 288, 2503
Eco31I GGTCTC 1 cut(s) 2688
Eco32I GATATC 2 cut(s) 347, 2094
Eco47I GGWCC 5 cut(s) 68, 1195, 2162, 2597, 2900
Eco57I CTGAAG 4 cut(s) 1596, 1716, 2915, 3113
EcoO109I RGGNCCY 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 4 cut(s) 189, 1197, 1960, 2677
EcoRV GATATC 2 cut(s) 347, 2094
EcoT14I CCWWGG 2 cut(s) 288, 2503
EcoT22I ATGCAT 2 cut(s) 1536, 2862
EgeI GGCGCC 1 cut(s) 1629
EheI GGCGCC 1 cut(s) 1629
ErhI CCWWGG 2 cut(s) 288, 2503
FalI AAGNNNNNCTT 4 cut(s) 1322, 1354, 3003, 3035
FaqI GGGAC 2 cut(s) 2175, 2886
FauI CCCGC 1 cut(s) 2911
FauNDI CATATG 1 cut(s) 2891
FblI GTMKAC 1 cut(s) 1150
FokI GGATG 6 cut(s) 155, 173, 179, 1529, 1533, 2488
FspBI CTAG 7 cut(s) 437, 1229, 1491, 2453, 2648, 2777, 3355
GlaI GCGC 2 cut(s) 615, 1629
GsuI CTGGAG 3 cut(s) 1155, 1706, 2789
HaeII RGCGCY 2 cut(s) 617, 1631
HaeIII GGCC 2 cut(s) 5, 449
HapII CCGG 2 cut(s) 1396, 1546
HhaI GCGC 2 cut(s) 616, 1630
Hin1I GRCGYC 2 cut(s) 1628, 2016
Hin6I GCGC 2 cut(s) 614, 1628
HinP1I GCGC 2 cut(s) 614, 1628
HincII GTYRAC 2 cut(s) 27, 502
HindII GTYRAC 2 cut(s) 27, 502
HindIII AAGCTT 5 cut(s) 369, 1034, 1869, 1931, 3197
HpaII CCGG 2 cut(s) 1396, 1546
HphI GGTGA 6 cut(s) 694, 967, 1849, 2443, 2678, 3269
Hpy166II GTNNAC 7 cut(s) 27, 502, 649, 889, 1101, 1151, 3064
Hpy8I GTNNAC 7 cut(s) 27, 502, 649, 889, 1101, 1151, 3064
HpyCH4III ACNGT 5 cut(s) 1468, 2126, 2536, 2951, 3079
HpyCH4IV ACGT 6 cut(s) 134, 1401, 1957, 2016, 2346, 3319
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 578, 660, 701, 833, 1166, 2912, 3140
HpySE526I ACGT 6 cut(s) 134, 1401, 1957, 2016, 2346, 3319
Hsp92I GRCGYC 2 cut(s) 1628, 2016
HspAI GCGC 2 cut(s) 614, 1628
KasI GGCGCC 1 cut(s) 1627
Kzo9I GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
LguI GCTCTTC 1 cut(s) 826
LmnI GCTCC 4 cut(s) 418, 578, 2390, 2911
LweI GCATC 9 cut(s) 532, 1417, 1521, 2309, 2536, 2635, 2807, 2847, 2987
MaeI CTAG 7 cut(s) 437, 1229, 1491, 2453, 2648, 2777, 3355
MaeII ACGT 6 cut(s) 134, 1401, 1957, 2016, 2346, 3319
MaeIII GTNAC 9 cut(s) 532, 1462, 2071, 2431, 2480, 2511, 2536, 2670, 3320
MalI GATC 9 cut(s) 101, 857, 1406, 1763, 1809, 2358, 2371, 3222, 3297
MbiI CCGCTC 1 cut(s) 2906
MboI GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
MfeI CAATTG 3 cut(s) 546, 3005, 3135
MflI RGATCY 4 cut(s) 99, 1761, 1807, 2369
MhlI GDGCHC 1 cut(s) 1183
MlsI TGGCCA 1 cut(s) 449
MluNI TGGCCA 1 cut(s) 449
Mly113I GGCGCC 1 cut(s) 1628
MlyI GAGTC 4 cut(s) 212, 478, 1106, 2935
MmeI TCCRAC 3 cut(s) 2702, 3021, 3310
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 2 cut(s) 1536, 2862
MroXI GAANNNNTTC 2 cut(s) 1087, 3015
MscI TGGCCA 1 cut(s) 449
MseI TTAA 9 cut(s) 129, 198, 723, 804, 1298, 1616, 1796, 2385, 2783
MslI CAYNNNNRTG 1 cut(s) 2489
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 4 cut(s) 36, 1220, 2466, 2959
MspI CCGG 2 cut(s) 1396, 1546
MspR9I CCNGG 5 cut(s) 191, 1199, 1547, 1962, 2679
MunI CAATTG 3 cut(s) 546, 3005, 3135
Mva1269I GAATGC 1 cut(s) 1750
MvaI CCWGG 4 cut(s) 191, 1199, 1962, 2679
MvnI CGCG 1 cut(s) 1635
MwoI GCNNNNNNNGC 8 cut(s) 248, 578, 660, 701, 833, 1166, 2912, 3140
NarI GGCGCC 1 cut(s) 1628
NciI CCSGG 1 cut(s) 1547
NcoI CCATGG 2 cut(s) 288, 2503
NdeI CATATG 1 cut(s) 2891
NdeII GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
NlaIV GGNNCC 5 cut(s) 69, 1629, 2163, 2902, 3248
NmuCI GTSAC 3 cut(s) 2431, 2480, 3320
NsiI ATGCAT 2 cut(s) 1536, 2862
NspI RCATGY 2 cut(s) 692, 2915
NspV TTCGAA 2 cut(s) 363, 3228
PagI TCATGA 1 cut(s) 858
PciI ACATGT 1 cut(s) 688
PciSI GCTCTTC 1 cut(s) 826
PctI GAATGC 1 cut(s) 1750
PdmI GAANNNNTTC 2 cut(s) 1087, 3015
PfeI GAWTC 6 cut(s) 404, 515, 928, 1052, 1783, 3112
PflFI GACNNNGTC 1 cut(s) 893
PflMI CCANNNNNTGG 1 cut(s) 655
PfoI TCCNGGA 1 cut(s) 1545
PleI GAGTC 4 cut(s) 211, 478, 1105, 2934
PluTI GGCGCC 1 cut(s) 1631
PpsI GAGTC 4 cut(s) 211, 478, 1105, 2934
Ppu21I YACGTR 3 cut(s) 1402, 2347, 3320
PpuMI RGGWCCY 1 cut(s) 68
PscI ACATGT 1 cut(s) 688
Psp1406I AACGTT 1 cut(s) 134
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 189, 1197, 1960, 2677
PspGI CCWGG 4 cut(s) 189, 1197, 1960, 2677
PspN4I GGNNCC 5 cut(s) 69, 1629, 2163, 2902, 3248
PspPI GGNCC 5 cut(s) 68, 1195, 2162, 2597, 2900
PspPPI RGGWCCY 1 cut(s) 68
PstI CTGCAG 1 cut(s) 710
PstNI CAGNNNCTG 5 cut(s) 827, 1786, 2322, 2540, 2962
PsuI RGATCY 4 cut(s) 99, 1761, 1807, 2369
PsyI GACNNNGTC 1 cut(s) 893
PvuII CAGCTG 3 cut(s) 36, 1220, 2959
RsaI GTAC 9 cut(s) 718, 781, 1400, 1478, 2123, 2327, 2349, 2404, 2751
RsaNI GTAC 9 cut(s) 717, 780, 1399, 1477, 2122, 2326, 2348, 2403, 2750
RseI CAYNNNNRTG 1 cut(s) 2489
SapI GCTCTTC 1 cut(s) 826
SaqAI TTAA 9 cut(s) 129, 198, 723, 804, 1298, 1616, 1796, 2385, 2783
Sau3AI GATC 9 cut(s) 99, 855, 1404, 1761, 1807, 2356, 2369, 3220, 3295
Sau96I GGNCC 5 cut(s) 68, 1195, 2162, 2597, 2900
SchI GAGTC 4 cut(s) 212, 478, 1106, 2935
ScrFI CCNGG 5 cut(s) 191, 1199, 1547, 1962, 2679
SduI GDGCHC 1 cut(s) 1183
SfaNI GCATC 9 cut(s) 532, 1417, 1521, 2309, 2536, 2635, 2807, 2847, 2987
SfcI CTRYAG 1 cut(s) 706
SfoI GGCGCC 1 cut(s) 1629
SfuI TTCGAA 2 cut(s) 363, 3228
SinI GGWCC 5 cut(s) 68, 1195, 2162, 2597, 2900
SmiMI CAYNNNNRTG 1 cut(s) 2489
SmlI CTYRAG 4 cut(s) 1031, 1328, 2522, 2696
SmoI CTYRAG 4 cut(s) 1031, 1328, 2522, 2696
SnaBI TACGTA 1 cut(s) 2347
SsiI CCGC 7 cut(s) 523, 660, 1635, 2429, 2464, 2469, 2904
SspDI GGCGCC 1 cut(s) 1627
SspI AATATT 2 cut(s) 2182, 2226
SspMI CTAG 7 cut(s) 437, 1229, 1491, 2453, 2648, 2777, 3355
StyD4I CCNGG 5 cut(s) 189, 1197, 1545, 1960, 2677
StyI CCWWGG 2 cut(s) 288, 2503
TaaI ACNGT 5 cut(s) 1468, 2126, 2536, 2951, 3079
TaiI ACGT 6 cut(s) 137, 1404, 1960, 2019, 2349, 3322
TatI WGTACW 2 cut(s) 2121, 2749
TauI GCSGC 1 cut(s) 663
TfiI GAWTC 6 cut(s) 404, 515, 928, 1052, 1783, 3112
Tru1I TTAA 9 cut(s) 129, 198, 723, 804, 1298, 1616, 1796, 2385, 2783
Tru9I TTAA 9 cut(s) 129, 198, 723, 804, 1298, 1616, 1796, 2385, 2783
TscAI CASTG 6 cut(s) 1473, 1660, 1671, 1825, 2607, 3066
TseFI GTSAC 3 cut(s) 2431, 2480, 3320
Tsp45I GTSAC 3 cut(s) 2431, 2480, 3320
TspGWI ACGGA 1 cut(s) 1819
TspRI CASTG 6 cut(s) 1473, 1660, 1671, 1825, 2607, 3066
Tth111I GACNNNGTC 1 cut(s) 893
Van91I CCANNNNNTGG 1 cut(s) 655
VpaK11BI GGWCC 5 cut(s) 68, 1195, 2162, 2597, 2900
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 2 cut(s) 692, 2915
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 2 cut(s) 1087, 3015
XspI CTAG 7 cut(s) 437, 1229, 1491, 2453, 2648, 2777, 3355
ZraI GACGTC 1 cut(s) 2017
Zsp2I ATGCAT 2 cut(s) 1536, 2862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.