Rw5G047710

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000379
Physical Location & Seq
Reverse (-)
82708929 .. 82710098
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047710.1

Sequence Viewer

Length: 1170 bp
ATGAATGAGGTTATCGAGGCCCTTAAAGATGACGAGGTCACTGCCATTGGTGTCTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATATCGGTGCACATGCCCGAAAAAGAGGGATTTTTGATTATGTGATTATGGCAGTCGTATCCCAAAGCCCTGACTTGGAAAAGATTCAAGGCGCATTGGCAGAGCTCCTGGGCTTCAAATTACATGAGAAGACAGAAATTGGAAGAGCAGCTAGACTGAGTAAGGAGATAATGAGAAGGAGAAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGACATATCAAGAATAGGAATACCAAGCTACAAGGAACTTCAAAAGTGCAATTCCAAAGTCCTACTCACCACAAGGATAAGAAATGTGTGTCATGTCATGAGATGCCAAGAAAAGATCACCCTCAGTACCCTATCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCCCCAATTTCGAGGATGTAGCGAGGTGGGTAGCTGGAGAGTGTTGTGGTTTACCGATTGCATTGATAGCAGTCGCAAGGGCACTTGGAGATAAAGAACTGGTGGAATGGAAAAGAGCAGCTCAACGACTAGAGAAGTCGCAATCTGCCAACCCGGACGATTCCAGAGAAGCATCCAAATGTATAAAATTAAGCTATGATTACCTGAAAGATGAGGACTACAAGTCATACTTCTTGCTCTGTTGCCTATTCCCAGAAGACTATAACATCCCAATAGAAGACTTGTTCAGGTACGCGATCGGGAAAGGATTGTTTCGAGATGCTGACACACTCGAAGAAGCCAGAGAGAGAGCTGTTTCCGTGGCCACACACCTTAAACATTCTTGCTTGCTTTTGGATACTGGAGAAAAATATGGATCTGGATCTGTAAAGATGCATGATGTTGTTCGAGATACAGCCATTCAAATTGCGCAATCTGAACATGGGTTTTTGGTGAGAGCTGGCTGTGATTTAGAGGACTGGCCACGTCAATTACATGAAGGCTATTCTGCAGTCTCACTAATGCACAATCATATTACCAAGCTACCCGAAAAGTTTGTGGGTTATTATCACAAATGGTACCTGAACTATACCTCAATCTTATCGATGGTATCTGAACTTCAATTTTGA

Protein Analysis

389

Amino Acids

44.29

Weight (kDa)

6.48

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 163 6.1e-33 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
Acc16I TGCGCA 1 cut(s) 972
Acc65I GGTACC 1 cut(s) 1119
AccB1I GGYRCC 1 cut(s) 1119
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 797
AclWI GGATC 3 cut(s) 274, 925, 931
AcoI YGGCCR 2 cut(s) 864, 1022
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 3 cut(s) 442, 794, 1121
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 6 cut(s) 182, 211, 356, 500, 965, 1163
AhdI GACNNNNNGTC 1 cut(s) 724
AjiI CACGTC 1 cut(s) 1028
AjnI CCWGG 2 cut(s) 201, 291
AluBI AGCT 9 cut(s) 199, 245, 342, 536, 623, 696, 854, 1001, 1084
AluI AGCT 9 cut(s) 199, 245, 342, 536, 623, 696, 854, 1001, 1084
Alw21I GWGCWC 2 cut(s) 106, 201
Alw26I GTCTC 1 cut(s) 1060
Alw44I GTGCAC 1 cut(s) 102
AlwI GGATC 3 cut(s) 274, 925, 931
AoxI GGCC 3 cut(s) 18, 864, 1022
ApaLI GTGCAC 1 cut(s) 102
ApeKI GCWGC 2 cut(s) 242, 620
Asp700I GAANNNNTTC 1 cut(s) 177
Asp718I GGTACC 1 cut(s) 1119
AspLEI GCGC 2 cut(s) 188, 973
AspS9I GGNCC 2 cut(s) 19, 465
AsuC2I CCSGG 1 cut(s) 656
AsuHPI GGTGA 3 cut(s) 373, 424, 1006
AvaII GGWCC 1 cut(s) 465
BaeGI GKGCMC 2 cut(s) 106, 586
BalI TGGCCA 2 cut(s) 866, 1024
BanI GGYRCC 1 cut(s) 1119
BanII GRGCYC 1 cut(s) 201
BarI GAAGNNNNNNTAC 1 cut(s) 1143
BbsI GAAGAC 3 cut(s) 230, 765, 786
Bbv12I GWGCWC 2 cut(s) 106, 201
BbvI GCAGC 2 cut(s) 254, 632
BccI CCATC 1 cut(s) 1141
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciT130I CCWGG 2 cut(s) 203, 293
BciVI GTATCC 2 cut(s) 163, 892
BcnI CCSGG 1 cut(s) 656
BcoDI GTCTC 1 cut(s) 1060
BfaI CTAG 2 cut(s) 246, 632
BfmI CTRYAG 1 cut(s) 1050
BfuI GTATCC 2 cut(s) 163, 892
BisI GCNGC 2 cut(s) 243, 621
BlsI GCNGC 2 cut(s) 244, 622
Bme1390I CCNGG 3 cut(s) 203, 293, 656
Bme18I GGWCC 1 cut(s) 465
BmeRI GACNNNNNGTC 1 cut(s) 724
BmgBI CACGTC 1 cut(s) 1028
BmgT120I GGNCC 2 cut(s) 19, 465
BmiI GGNNCC 1 cut(s) 1121
BmrFI CCNGG 3 cut(s) 203, 293, 656
BmsI GCATC 4 cut(s) 407, 683, 811, 924
BpiI GAAGAC 3 cut(s) 230, 765, 786
BpmI CTGGAG 2 cut(s) 558, 924
BpuMI CCSGG 1 cut(s) 656
Bsa29I ATCGAT 1 cut(s) 1145
BsaBI GATNNNNATC 1 cut(s) 922
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 84, 202, 861
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 606, 907, 1025
Bse8I GATNNNNATC 1 cut(s) 922
BseBI CCWGG 2 cut(s) 203, 293
BseCI ATCGAT 1 cut(s) 1145
BseDI CCNNGG 3 cut(s) 84, 202, 861
BseGI GGATG 3 cut(s) 523, 674, 768
BseJI GATNNNNATC 1 cut(s) 922
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 242, 451
BseNI ACTGG 3 cut(s) 606, 907, 1025
BseSI GKGCMC 2 cut(s) 106, 586
BseXI GCAGC 2 cut(s) 254, 632
Bsh1236I CGCG 1 cut(s) 797
Bsh1285I CGRYCG 1 cut(s) 801
BshFI GGCC 3 cut(s) 20, 866, 1024
BshNI GGYRCC 1 cut(s) 1119
BshVI ATCGAT 1 cut(s) 1145
BsiEI CGRYCG 1 cut(s) 801
BsiHKAI GWGCWC 2 cut(s) 106, 201
BsiSI CCGG 1 cut(s) 656
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 1 cut(s) 1060
BsnI GGCC 3 cut(s) 20, 866, 1024
Bsp1286I GDGCHC 3 cut(s) 106, 201, 586
Bsp143I GATC 5 cut(s) 279, 429, 798, 917, 923
Bsp19I CCATGG 1 cut(s) 84
BspANI GGCC 3 cut(s) 20, 866, 1024
BspCNI CTCAG 2 cut(s) 243, 450
BspDI ATCGAT 1 cut(s) 1145
BspFNI CGCG 1 cut(s) 797
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 1121
BspMAI CTGCAG 1 cut(s) 1054
BspPI GGATC 3 cut(s) 274, 925, 931
BspQI GCTCTTC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 1119
BsrI ACTGG 3 cut(s) 606, 907, 1025
BssECI CCNNGG 3 cut(s) 84, 202, 861
BssMI GATC 5 cut(s) 279, 429, 798, 917, 923
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 203, 293
Bst6I CTCTTC 1 cut(s) 232
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 890, 1003
BstDEI CTNAG 2 cut(s) 251, 437
BstDSI CCRYGG 2 cut(s) 84, 861
BstF5I GGATG 3 cut(s) 523, 674, 768
BstFNI CGCG 1 cut(s) 797
BstHHI GCGC 2 cut(s) 188, 973
BstKTI GATC 5 cut(s) 282, 432, 801, 920, 926
BstMAI GTCTC 1 cut(s) 1060
BstMBI GATC 5 cut(s) 279, 429, 798, 917, 923
BstMCI CGRYCG 1 cut(s) 801
BstMWI GCNNNNNNNGC 2 cut(s) 66, 569
BstNI CCWGG 2 cut(s) 203, 293
BstNSI RCATGY 1 cut(s) 110
BstSCI CCNGG 3 cut(s) 201, 291, 654
BstSFI CTRYAG 1 cut(s) 1050
BstSLI GKGCMC 2 cut(s) 106, 586
BstUI CGCG 1 cut(s) 797
BstV1I GCAGC 2 cut(s) 254, 632
BstV2I GAAGAC 3 cut(s) 230, 765, 786
BstX2I RGATCY 3 cut(s) 279, 917, 923
BstYI RGATCY 3 cut(s) 279, 917, 923
Bsu15I ATCGAT 1 cut(s) 1145
BsuI GTATCC 2 cut(s) 163, 892
BsuRI GGCC 3 cut(s) 20, 866, 1024
BsuTUI ATCGAT 1 cut(s) 1145
BtgI CCRYGG 2 cut(s) 84, 861
BtrI CACGTC 1 cut(s) 1028
BtsCI GGATG 3 cut(s) 523, 674, 768
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 890, 1003
CciI TCATGA 1 cut(s) 411
CfoI GCGC 2 cut(s) 188, 973
Cfr13I GGNCC 2 cut(s) 19, 465
ClaI ATCGAT 1 cut(s) 1145
CseI GACGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 441, 793, 1120
CviAII CATG 9 cut(s) 61, 85, 107, 218, 407, 412, 938, 983, 1037
CviQI GTAC 3 cut(s) 441, 793, 1120
DdeI CTNAG 2 cut(s) 251, 437
DpnI GATC 5 cut(s) 281, 431, 800, 919, 925
DpnII GATC 5 cut(s) 279, 429, 798, 917, 923
DriI GACNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 2 cut(s) 864, 1022
Eam1104I CTCTTC 1 cut(s) 232
Eam1105I GACNNNNNGTC 1 cut(s) 724
EarI CTCTTC 1 cut(s) 232
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 19
EcoRII CCWGG 2 cut(s) 201, 291
EcoT14I CCWWGG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 939
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 9 cut(s) 64, 88, 110, 221, 410, 415, 941, 986, 1040
FalI AAGNNNNNCTT 2 cut(s) 716, 748
FatI CATG 9 cut(s) 60, 84, 106, 217, 406, 411, 937, 982, 1036
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 243, 621
FokI GGATG 3 cut(s) 530, 661, 755
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 2 cut(s) 243, 621
FspBI CTAG 2 cut(s) 246, 632
FspI TGCGCA 1 cut(s) 972
GlaI GCGC 2 cut(s) 187, 972
GluI GCNGC 2 cut(s) 243, 621
GsuI CTGGAG 2 cut(s) 558, 924
HaeIII GGCC 3 cut(s) 20, 866, 1024
HapII CCGG 1 cut(s) 656
HgaI GACGC 1 cut(s) 58
HhaI GCGC 2 cut(s) 188, 973
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 9 cut(s) 64, 88, 110, 221, 410, 415, 941, 986, 1040
Hin6I GCGC 2 cut(s) 186, 971
HinP1I GCGC 2 cut(s) 186, 971
HinfI GANTC 4 cut(s) 178, 458, 500, 662
HpaII CCGG 1 cut(s) 656
HphI GGTGA 3 cut(s) 373, 424, 1006
Hpy166II GTNNAC 3 cut(s) 55, 104, 554
Hpy188I TCNGA 3 cut(s) 451, 979, 1156
Hpy188III TCNNGA 7 cut(s) 324, 412, 666, 802, 818, 921, 950
Hpy8I GTNNAC 3 cut(s) 55, 104, 554
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 264, 483, 1034
HpyCH4IV ACGT 1 cut(s) 1027
HpyCH4V TGCA 7 cut(s) 104, 363, 485, 563, 937, 1052, 1066
HpyF10VI GCNNNNNNNGC 2 cut(s) 66, 569
HpyF3I CTNAG 2 cut(s) 251, 437
HpySE526I ACGT 1 cut(s) 1027
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 9 cut(s) 64, 88, 110, 221, 410, 415, 941, 986, 1040
HspAI GCGC 2 cut(s) 186, 971
KpnI GGTACC 1 cut(s) 1123
Kzo9I GATC 5 cut(s) 279, 429, 798, 917, 923
LguI GCTCTTC 1 cut(s) 232
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 2 cut(s) 254, 632
LweI GCATC 4 cut(s) 407, 683, 811, 924
MaeI CTAG 2 cut(s) 246, 632
MaeII ACGT 1 cut(s) 1027
MaeIII GTNAC 1 cut(s) 37
MalI GATC 5 cut(s) 281, 431, 800, 919, 925
MboI GATC 5 cut(s) 279, 429, 798, 917, 923
MboII GAAGA 7 cut(s) 235, 249, 464, 467, 770, 791, 848
MflI RGATCY 3 cut(s) 279, 917, 923
MhlI GDGCHC 3 cut(s) 106, 201, 586
MlsI TGGCCA 2 cut(s) 866, 1024
MluCI AATT 8 cut(s) 212, 231, 364, 508, 689, 966, 1031, 1163
MluNI TGGCCA 2 cut(s) 866, 1024
Mox20I TGGCCA 2 cut(s) 866, 1024
Mph1103I ATGCAT 1 cut(s) 939
MroXI GAANNNNTTC 1 cut(s) 177
MscI TGGCCA 2 cut(s) 866, 1024
MseI TTAA 3 cut(s) 24, 692, 876
MslI CAYNNNNRTG 2 cut(s) 99, 679
Msp20I TGGCCA 2 cut(s) 866, 1024
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 3 cut(s) 203, 293, 656
MvaI CCWGG 2 cut(s) 203, 293
MvnI CGCG 1 cut(s) 797
MwoI GCNNNNNNNGC 2 cut(s) 66, 569
NciI CCSGG 1 cut(s) 656
NcoI CCATGG 1 cut(s) 84
NdeII GATC 5 cut(s) 279, 429, 798, 917, 923
NlaIII CATG 9 cut(s) 64, 88, 110, 221, 410, 415, 941, 986, 1040
NlaIV GGNNCC 1 cut(s) 1121
NmuCI GTSAC 1 cut(s) 37
NsbI TGCGCA 1 cut(s) 972
NsiI ATGCAT 1 cut(s) 939
NspI RCATGY 1 cut(s) 110
PagI TCATGA 1 cut(s) 411
PciSI GCTCTTC 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 4 cut(s) 178, 458, 500, 662
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 2 cut(s) 244, 622
Ple19I CGATCG 1 cut(s) 801
PsiI TTATAA 1 cut(s) 287
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 201, 291
PspGI CCWGG 2 cut(s) 201, 291
PspN4I GGNNCC 1 cut(s) 1121
PspPI GGNCC 2 cut(s) 19, 465
PstI CTGCAG 1 cut(s) 1054
PsuI RGATCY 3 cut(s) 279, 917, 923
PsyI GACNNNGTC 1 cut(s) 35
PvuI CGATCG 1 cut(s) 801
RsaI GTAC 3 cut(s) 442, 794, 1121
RsaNI GTAC 3 cut(s) 441, 793, 1120
RseI CAYNNNNRTG 2 cut(s) 99, 679
SacI GAGCTC 1 cut(s) 201
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 3 cut(s) 24, 692, 876
SatI GCNGC 2 cut(s) 243, 621
Sau3AI GATC 5 cut(s) 279, 429, 798, 917, 923
Sau96I GGNCC 2 cut(s) 19, 465
ScrFI CCNGG 3 cut(s) 203, 293, 656
SduI GDGCHC 3 cut(s) 106, 201, 586
SfaNI GCATC 4 cut(s) 407, 683, 811, 924
SfcI CTRYAG 1 cut(s) 1050
SinI GGWCC 1 cut(s) 465
SmiMI CAYNNNNRTG 2 cut(s) 99, 679
Sse9I AATT 8 cut(s) 212, 231, 364, 508, 689, 966, 1031, 1163
SspMI CTAG 2 cut(s) 246, 632
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 3 cut(s) 201, 291, 654
StyI CCWWGG 1 cut(s) 84
TaiI ACGT 1 cut(s) 1030
TaqI TCGA 6 cut(s) 15, 513, 817, 834, 949, 1145
TasI AATT 8 cut(s) 212, 231, 364, 508, 689, 966, 1031, 1163
TfiI GAWTC 4 cut(s) 178, 458, 500, 662
Tru1I TTAA 3 cut(s) 24, 692, 876
Tru9I TTAA 3 cut(s) 24, 692, 876
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 2 cut(s) 242, 620
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 17, 1053
TspGWI ACGGA 1 cut(s) 850
TspRI CASTG 1 cut(s) 46
Tth111I GACNNNGTC 1 cut(s) 35
VneI GTGCAC 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 465
XceI RCATGY 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 54
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 2 cut(s) 246, 632
Zsp2I ATGCAT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.