FvH4_3g44610

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37160003 .. 37163530
3528 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44610.t1

Sequence Viewer

Length: 3225 bp
ATGGATTTCGATTTCTTCGAGATATCGTTGGCGATTGTTGCGGTAATTGTGACATGGACTCAAGTAAAGCGTCGGATAAGTTACACGGTTGGAAAGTCGCAGGATAATCAAGTTGTGGTTGACATGGAGGCCTCTGCATCTCTTCCTTCTTCATCGTCAGCAGTACTTGAATGCAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAAGGCATTACAGCCCAAATACGCGATCGACTTCGTAGAAGTGGCATTATAACGTTCATGGATGAACCAGATCTTAGAGTAGGGGTTACCATTTCGCCCACTCTCATAAAGGCAATTAAAGAGTCGAGGTTTGCAATCGTAATTCTCTCACAAAATTATGCTTCTTCTATTTGGTGTTTGGAGGAACTTGGAGAGATTTGTCTGTCCAAGGAAGACAACAGAATTTTGCCACTTTTTTTTGAAGTTGATCCTACTGATGTTCGACATCAGAAGAAGAGTTTCGAAGAAGCTTTCTCTAAGCATGAAACCTCTGGCCGACATGAATCAGACAAGTTGAATCAATGGAGGGCTGCCTTAAGCAGAATGGCCAAGATCTCAGGGTGGAACACAAGTGATTATAAGACTCACGAAGAACTTGTTGACAACATTGTGGAATCTCTCCGCAGTAAAGTACCAGCTGATGCAATTGAATTCACAAATGATTTCCAAGCATTTGGAGCAACAAAAGAAGCGACAAACAAGGTTATGAAGGCGCTAACAGATGACAAGATTACTGCCGTTGGTGTCTATGGAATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTGGCTGCACAAGCCTGCAGAAATGGTACTTTTAACCATTGGATTATTGCTGTCGTATCCCAAAACCTTGACTTGAAAATAGTTCAAGGTACATTGGCAGATTCGTTGGGCTTTAAATTCGTGGAGGAGACACAAGCTGGAAGAGCTGATAGGTTGCATAAGGAGATAATGGGAAAAGAAAAGCTCCTTATAATCTTGGACGATGTATGGGAGAGAACCGAGTTGTCAAAGATAGGGATTCCCACCTACAATGAGCTTGAAAAGTGCAAGTCCAAAGTGCTACTCACCACCAGGAGATTGAATGTCTGTAATGCCATGAAGTGCCATGAAAAGATCAGCCTCAAAGTTCTCTCAGAACAAGATTCTTGGACCTTGTTCGTGAGAAATGCAGGAACAATTTCTTTTGAGCCTACCTCATTTGAGAAAGTGGCAAGGAGGGTAGCTTTAGAGTGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCAGAGATGAGGACCAGGAGGAATGGGAGAAGGCAGCTGAACGACTAGAGCAGTCGCAATATGTCAATCCCAACCATGAGAAAGATGAGGATAATGCTTTCAGATGTATACGATTAAGCTATGATTACTTGAAAAATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCATAACATCAAAATAAAAGACTTGTTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGCCCAAACAATGGAAGTAGCCAGAGGAAAATTAGTTACAGTGGTCAAGTACCTGAAAGGTTCTAGCTTGCTTTTGGATAGTGAGGAAGAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCCGAATCTGAAGATTGCCATTGGTTTTTTGTGAAAGCTGGCTGTGGTTTAAAGGGTTGGCCGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACGAAATTCGGAAGCTACCCGAAGAAGAGTTAGTATGTCCAAATCTCCTGATTTTATTACTAAACGGGAATGCTGAACTAAGTGAGATCCCCGAAAAGTTGATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCCGTATTACCCCAATCATTTAGTCTTCTCACCAACCTCCAAGCTTTGTATTTAGATGATTGCGGCGAATTAATTGACATTTCCGTTGTGGGGAAACTTAACAAGCTTGAAATTCTTAGTATGAGACCCATCTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTACTGACACTCGCAAAGGGATTGCAAACGACATCTGCAATCGACTTAGTAGAAGTGGCATTAAAACGTTCATGGATGAGCAAGGACTTCAAGTAGGGGATGTTATTTCTCCCACTCTCATATGGGCAATTAAAAAGGCAAGGTTTGCAATCGTAATTTTCTCACAAAATTACGCTTCTTCTGCTTGGTGTTTGGATGAACTTCGAGAGATTTGTCTATCCATAGAAGACGGCAAAATTTTGCCACTTTTTTATCAAGTTAATCCTAGTGATGTTCAATATCACGGGAACAATTTCAAAGAAGCTTTCTCTAAGCATGAAACCTCTGGCCGACATGAATCAGACAAGTTGAATCAATGGAGGGCTGCCTTATGCAAAGTGACCAATATCTCTGGGTGGAATACAAATGATTTCAATACTCACAGTGAACTTGTTGACAAGATTGTGGAATCTCTCCGCAGTAAAGTACCACATGATCCAATTGAATCCACAGTAGATTTCCAAGCATATGAAGCAACAAAAGAAGCCATGCGCAAGGTTATGGAGGCGCTAACAGATGACAAGATTACTGCCGTTGGTGTCCACGGCACAGGCAGCGTTGGGAAGACAAGCATGTTGAGACATGTAGCTACACAAGCATGCAGAAATGGTAATTTTAACAATTGGATTATGGTTGTCGTATCCCAAGACCTTGACTTGAAAAAAATTGAAGGTACATTTTCAAATAAGTTGCTCTTCAAATTTGCGAAGAAGACAGAAGATGTAAGAGCTGATAGGTTACATGAGGAGATAATGAGAACAGAAAAGCTCCTTATAATCTTGGACGATGTATGGGATAAAATACAGTTGTCAAAGATAGGGATCCCTAACTACGAAGAGCTTCAAAAATGCAATTCCAAAGTCCTACTCACCACAAGGAGATTGAGTGTCTGTAATGCTATGGAGTGCCATGAAAAAATCGCCCTCAACATTCTATCAAAAGAAGATTCTTGGGCCTTGTTTGTGAGAAATATAGGAACAATTTCTTTTGAGTCCACCTTGTTTAAGGAAGTGGCATGGAGGGTAGCTGGAGAGTGGAAGGGTCTACCGATTGCATTGTAA

Protein Analysis

1075

Amino Acids

121.72

Weight (kDa)

6.35

Isoelectric Point (pI)

36.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 59 - 222 5.6e-42 TIR domain
TIR_2 PF13676 62 - 156 1.3e-14 TIR domain
NB-ARC PF00931 242 - 405 1e-29 NB-ARC domain
LRR_8 PF13855 617 - 675 2e-08 Leucine rich repeat
LRR_14 PF23598 627 - 698 4.1e-09 Leucine-rich repeat region
TIR PF01582 700 - 864 8e-39 TIR domain
TIR_2 PF13676 703 - 797 1.1e-13 TIR domain
NB-ARC PF00931 882 - 1044 3e-29 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 266, 615, 1016, 2939
Acc16I TGCGCA 1 cut(s) 2657
AccB7I CCANNNNNTGG 1 cut(s) 1573
AccI GTMKAC 3 cut(s) 1285, 1426, 3208
AccII CGCG 3 cut(s) 240, 1538, 1770
AciI CCGC 7 cut(s) 41, 658, 795, 1763, 1770, 2025, 2581
AclI AACGTT 2 cut(s) 269, 2192
AclWI GGATC 7 cut(s) 458, 1891, 1906, 1950, 2594, 2980, 2993
AcoI YGGCCR 4 cut(s) 529, 582, 1760, 2452
AcsI RAATTY 7 cut(s) 438, 686, 941, 1815, 2073, 2361, 2864
AcuI CTGAAG 1 cut(s) 1731
AfaI GTAC 9 cut(s) 165, 669, 853, 916, 1535, 1613, 2128, 2592, 2839
AfiI CCNNNNNNNGG 3 cut(s) 1573, 1769, 2052
AflII CTTAAG 1 cut(s) 571
AflIII ACRYGT 2 cut(s) 823, 2746
AhdI GACNNNNNGTC 1 cut(s) 1465
AhlI ACTAGT 1 cut(s) 1955
AjnI CCWGG 2 cut(s) 1115, 1332
Alw26I GTCTC 4 cut(s) 814, 947, 2080, 2737
AlwI GGATC 7 cut(s) 458, 1891, 1906, 1950, 2594, 2980, 2993
AlwNI CAGNNNCTG 1 cut(s) 1921
AoxI GGCC 6 cut(s) 129, 529, 582, 1760, 2452, 3117
ApeKI GCWGC 6 cut(s) 566, 830, 1352, 1785, 2489, 2718
ApoI RAATTY 7 cut(s) 438, 686, 941, 1815, 2073, 2361, 2864
AseI ATTAAT 1 cut(s) 2033
Asp700I GAANNNNTTC 5 cut(s) 494, 1222, 2417, 3003, 3145
AspLEI GCGC 3 cut(s) 751, 2658, 2674
AspS9I GGNCC 4 cut(s) 203, 1194, 1330, 3117
AsuC2I CCSGG 1 cut(s) 1682
AsuHPI GGTGA 4 cut(s) 829, 1102, 1984, 3025
AsuII TTCGAA 1 cut(s) 498
AvaII GGWCC 3 cut(s) 203, 1194, 1330
BaeGI GKGCMC 1 cut(s) 1318
BalI TGGCCA 1 cut(s) 584
BamHI GGATCC 1 cut(s) 2985
BbsI GAAGAC 7 cut(s) 435, 1464, 1506, 1979, 2358, 2735, 2882
BbvI GCAGC 6 cut(s) 553, 817, 1364, 1772, 2476, 2730
BccI CCATC 1 cut(s) 2099
BceAI ACGGC 4 cut(s) 758, 2371, 2681, 2725
BciT130I CCWGG 2 cut(s) 1117, 1334
BciVI GTATCC 3 cut(s) 892, 1678, 2815
BcnI CCSGG 1 cut(s) 1682
BcoDI GTCTC 4 cut(s) 814, 947, 2080, 2737
BcuI ACTAGT 1 cut(s) 1955
BfaI CTAG 4 cut(s) 1364, 1626, 1956, 2391
BfmI CTRYAG 1 cut(s) 841
BfoI RGCGCY 2 cut(s) 752, 2675
BfrI CTTAAG 1 cut(s) 571
BfuI GTATCC 3 cut(s) 892, 1678, 2815
BglII AGATCT 2 cut(s) 286, 588
BisI GCNGC 9 cut(s) 567, 796, 831, 1353, 1763, 1786, 2026, 2490, 2719
BlsI GCNGC 9 cut(s) 568, 797, 832, 1354, 1764, 1787, 2027, 2491, 2720
BmcAI AGTACT 1 cut(s) 165
Bme1390I CCNGG 3 cut(s) 1117, 1334, 1682
Bme18I GGWCC 3 cut(s) 203, 1194, 1330
BmeRI GACNNNNNGTC 1 cut(s) 1465
BmgT120I GGNCC 4 cut(s) 203, 1194, 1330, 3117
BmiI GGNNCC 2 cut(s) 204, 2987
BmrFI CCNGG 3 cut(s) 1117, 1334, 1682
BmsI GCATC 4 cut(s) 146, 667, 1552, 1656
BpiI GAAGAC 7 cut(s) 435, 1464, 1506, 1979, 2358, 2735, 2882
BplI GAGNNNNNCTC 2 cut(s) 1223, 1255
BpmI CTGGAG 1 cut(s) 3213
Bpu14I TTCGAA 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 1682
BsaBI GATNNNNATC 1 cut(s) 2984
BsaI GGTCTC 1 cut(s) 2080
BsaJI CCNNGG 2 cut(s) 423, 2707
BsaWI WCCGGW 1 cut(s) 1530
BsaXI ACNNNNNCTCC 2 cut(s) 3034, 3064
Bsc4I CCNNNNNNNGG 3 cut(s) 1573, 1769, 2052
Bse8I GATNNNNATC 1 cut(s) 2984
BseBI CCWGG 2 cut(s) 1117, 1334
BseDI CCNNGG 2 cut(s) 423, 2707
BseGI GGATG 6 cut(s) 283, 1661, 1677, 2206, 2230, 2326
BseJI GATNNNNATC 1 cut(s) 2984
BseLI CCNNNNNNNGG 3 cut(s) 1573, 1769, 2052
BseMII CTCAG 3 cut(s) 606, 1191, 1333
BseRI GAGGAG 2 cut(s) 965, 2924
BseSI GKGCMC 1 cut(s) 1318
BseXI GCAGC 6 cut(s) 553, 817, 1364, 1772, 2476, 2730
BsgI GTGCAG 2 cut(s) 816, 1771
Bsh1236I CGCG 3 cut(s) 240, 1538, 1770
Bsh1285I CGRYCG 2 cut(s) 244, 1542
BshFI GGCC 6 cut(s) 131, 531, 584, 1762, 2454, 3119
BsiEI CGRYCG 2 cut(s) 244, 1542
BsiSI CCGG 2 cut(s) 1531, 1681
BslI CCNNNNNNNGG 3 cut(s) 1573, 1769, 2052
BsmAI GTCTC 4 cut(s) 814, 947, 2080, 2737
BsmI GAATGC 2 cut(s) 176, 1885
BsnI GGCC 6 cut(s) 131, 531, 584, 1762, 2454, 3119
Bso31I GGTCTC 1 cut(s) 2080
Bsp119I TTCGAA 1 cut(s) 498
Bsp1286I GDGCHC 1 cut(s) 1318
BspACI CCGC 7 cut(s) 41, 658, 795, 1763, 1770, 2025, 2581
BspANI GGCC 6 cut(s) 131, 531, 584, 1762, 2454, 3119
BspCNI CTCAG 3 cut(s) 605, 1190, 1332
BspFNI CGCG 3 cut(s) 240, 1538, 1770
BspLI GGNNCC 2 cut(s) 204, 2987
BspMAI CTGCAG 1 cut(s) 845
BspPI GGATC 7 cut(s) 458, 1891, 1906, 1950, 2594, 2980, 2993
BspQI GCTCTTC 3 cut(s) 961, 2864, 2994
BspT104I TTCGAA 1 cut(s) 498
BspTI CTTAAG 1 cut(s) 571
BspTNI GGTCTC 1 cut(s) 2080
BssECI CCNNGG 2 cut(s) 423, 2707
BssNAI GTATAC 1 cut(s) 1427
BssT1I CCWWGG 1 cut(s) 423
Bst1107I GTATAC 1 cut(s) 1427
Bst2UI CCWGG 2 cut(s) 1117, 1334
Bst4CI ACNGT 5 cut(s) 88, 1603, 2549, 2617, 2970
Bst6I CTCTTC 6 cut(s) 147, 485, 961, 1830, 2864, 2994
BstAFI CTTAAG 1 cut(s) 571
BstAPI GCANNNNNTGC 1 cut(s) 2270
BstBI TTCGAA 1 cut(s) 498
BstC8I GCNNGC 4 cut(s) 841, 1631, 1741, 2764
BstDSI CCRYGG 1 cut(s) 2707
BstEII GGTNACC 1 cut(s) 301
BstF5I GGATG 6 cut(s) 283, 1661, 1677, 2206, 2230, 2326
BstFNI CGCG 3 cut(s) 240, 1538, 1770
BstH2I RGCGCY 2 cut(s) 752, 2675
BstHHI GCGC 3 cut(s) 751, 2658, 2674
BstMAI GTCTC 4 cut(s) 814, 947, 2080, 2737
BstMCI CGRYCG 2 cut(s) 244, 1542
BstNI CCWGG 2 cut(s) 1117, 1334
BstNSI RCATGY 4 cut(s) 827, 2740, 2750, 2766
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 3 cut(s) 1115, 1332, 1680
BstSFI CTRYAG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 1318
BstUI CGCG 3 cut(s) 240, 1538, 1770
BstV1I GCAGC 6 cut(s) 553, 817, 1364, 1772, 2476, 2730
BstV2I GAAGAC 7 cut(s) 435, 1464, 1506, 1979, 2358, 2735, 2882
BstX2I RGATCY 5 cut(s) 286, 588, 1896, 1942, 2985
BstXI CCANNNNNNTGG 1 cut(s) 710
BstYI RGATCY 5 cut(s) 286, 588, 1896, 1942, 2985
BstZ17I GTATAC 1 cut(s) 1427
BsuI GTATCC 3 cut(s) 892, 1678, 2815
BsuRI GGCC 6 cut(s) 131, 531, 584, 1762, 2454, 3119
BtgI CCRYGG 1 cut(s) 2707
BtsCI GGATG 6 cut(s) 283, 1661, 1677, 2206, 2230, 2326
BtsI GCAGTG 1 cut(s) 1788
BtsIMutI CAGTG 3 cut(s) 1608, 1788, 2554
Cac8I GCNNGC 4 cut(s) 841, 1631, 1741, 2764
CaiI CAGNNNCTG 1 cut(s) 1921
CfoI GCGC 3 cut(s) 751, 2658, 2674
Cfr13I GGNCC 4 cut(s) 203, 1194, 1330, 3117
CseI GACGC 1 cut(s) 59
Csp6I GTAC 9 cut(s) 164, 668, 852, 915, 1534, 1612, 2127, 2591, 2838
CviQI GTAC 9 cut(s) 164, 668, 852, 915, 1534, 1612, 2127, 2591, 2838
DraI TTTAAA 2 cut(s) 940, 1752
DriI GACNNNNNGTC 1 cut(s) 1465
EaeI YGGCCR 4 cut(s) 529, 582, 1760, 2452
Eam1104I CTCTTC 6 cut(s) 147, 485, 961, 1830, 2864, 2994
Eam1105I GACNNNNNGTC 1 cut(s) 1465
EarI CTCTTC 6 cut(s) 147, 485, 961, 1830, 2864, 2994
Eco130I CCWWGG 1 cut(s) 423
Eco147I AGGCCT 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 2080
Eco32I GATATC 1 cut(s) 24
Eco47I GGWCC 3 cut(s) 203, 1194, 1330
Eco57I CTGAAG 1 cut(s) 1731
Eco91I GGTNACC 1 cut(s) 301
EcoO109I RGGNCCY 1 cut(s) 203
EcoO65I GGTNACC 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 2 cut(s) 1115, 1332
EcoRV GATATC 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 1671
ErhI CCWWGG 1 cut(s) 423
FalI AAGNNNNNCTT 4 cut(s) 1457, 1489, 2843, 2875
FauNDI CATATG 2 cut(s) 2246, 2632
FblI GTMKAC 3 cut(s) 1285, 1426, 3208
Fnu4HI GCNGC 9 cut(s) 567, 796, 831, 1353, 1763, 1786, 2026, 2490, 2719
FokI GGATG 6 cut(s) 290, 1664, 1668, 2213, 2237, 2333
Fsp4HI GCNGC 9 cut(s) 567, 796, 831, 1353, 1763, 1786, 2026, 2490, 2719
FspBI CTAG 4 cut(s) 1364, 1626, 1956, 2391
FspI TGCGCA 1 cut(s) 2657
GlaI GCGC 3 cut(s) 750, 2657, 2673
GluI GCNGC 9 cut(s) 567, 796, 831, 1353, 1763, 1786, 2026, 2490, 2719
GsuI CTGGAG 1 cut(s) 3213
HaeII RGCGCY 2 cut(s) 752, 2675
HaeIII GGCC 6 cut(s) 131, 531, 584, 1762, 2454, 3119
HapII CCGG 2 cut(s) 1531, 1681
HgaI GACGC 1 cut(s) 59
HhaI GCGC 3 cut(s) 751, 2658, 2674
Hin6I GCGC 3 cut(s) 749, 2656, 2672
HinP1I GCGC 3 cut(s) 749, 2656, 2672
HincII GTYRAC 3 cut(s) 121, 637, 2560
HindII GTYRAC 3 cut(s) 121, 637, 2560
HindIII AAGCTT 4 cut(s) 504, 2004, 2066, 2427
HpaII CCGG 2 cut(s) 1531, 1681
HphI GGTGA 4 cut(s) 829, 1102, 1984, 3025
Hpy166II GTNNAC 9 cut(s) 121, 637, 1286, 1427, 2552, 2560, 2707, 3159, 3209
Hpy188III TCNNGA 9 cut(s) 19, 206, 623, 1204, 1559, 1858, 1915, 2095, 2330
Hpy8I GTNNAC 9 cut(s) 121, 637, 1286, 1427, 2552, 2560, 2707, 3159, 3209
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 7 cut(s) 156, 739, 1342, 1646, 1775, 2828, 3196
HpyCH4III ACNGT 5 cut(s) 88, 1603, 2549, 2617, 2970
HpyCH4IV ACGT 2 cut(s) 269, 2192
HpySE526I ACGT 2 cut(s) 269, 2192
HspAI GCGC 3 cut(s) 749, 2656, 2672
LguI GCTCTTC 3 cut(s) 961, 2864, 2994
LmnI GCTCC 3 cut(s) 713, 1014, 2937
Lsp1109I GCAGC 6 cut(s) 553, 817, 1364, 1772, 2476, 2730
LweI GCATC 4 cut(s) 146, 667, 1552, 1656
MaeI CTAG 4 cut(s) 1364, 1626, 1956, 2391
MaeII ACGT 2 cut(s) 269, 2192
MaeIII GTNAC 6 cut(s) 49, 80, 301, 1597, 2503, 2901
MfeI CAATTG 3 cut(s) 681, 2604, 2785
MflI RGATCY 5 cut(s) 286, 588, 1896, 1942, 2985
MhlI GDGCHC 1 cut(s) 1318
MlsI TGGCCA 1 cut(s) 584
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 4 cut(s) 52, 347, 613, 3164
MmeI TCCRAC 3 cut(s) 53, 70, 781
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 1671
MroXI GAANNNNTTC 5 cut(s) 494, 1222, 2417, 3003, 3145
MscI TGGCCA 1 cut(s) 584
MslI CAYNNNNRTG 1 cut(s) 2761
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 674, 1355
MspCI CTTAAG 1 cut(s) 571
MspI CCGG 2 cut(s) 1531, 1681
MspR9I CCNGG 3 cut(s) 1117, 1334, 1682
MunI CAATTG 3 cut(s) 681, 2604, 2785
Mva1269I GAATGC 2 cut(s) 176, 1885
MvaI CCWGG 2 cut(s) 1117, 1334
MvnI CGCG 3 cut(s) 240, 1538, 1770
NciI CCSGG 1 cut(s) 1682
NdeI CATATG 2 cut(s) 2246, 2632
NlaIV GGNNCC 2 cut(s) 204, 2987
NmuCI GTSAC 2 cut(s) 49, 2503
NsbI TGCGCA 1 cut(s) 2657
NsiI ATGCAT 1 cut(s) 1671
NspI RCATGY 4 cut(s) 827, 2740, 2750, 2766
NspV TTCGAA 1 cut(s) 498
PaeI GCATGC 1 cut(s) 2766
PceI AGGCCT 1 cut(s) 131
PciI ACATGT 2 cut(s) 823, 2746
PciSI GCTCTTC 3 cut(s) 961, 2864, 2994
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 2 cut(s) 176, 1885
PdmI GAANNNNTTC 5 cut(s) 494, 1222, 2417, 3003, 3145
PflMI CCANNNNNTGG 1 cut(s) 1573
PfoI TCCNGGA 1 cut(s) 1680
PkrI GCNGC 9 cut(s) 568, 797, 832, 1354, 1764, 1787, 2027, 2491, 2720
Ple19I CGATCG 2 cut(s) 244, 1542
PleI GAGTC 4 cut(s) 52, 346, 613, 3163
PpsI GAGTC 4 cut(s) 52, 346, 613, 3163
PpuMI RGGWCCY 1 cut(s) 203
PscI ACATGT 2 cut(s) 823, 2746
PshBI ATTAAT 1 cut(s) 2033
PsiI TTATAA 4 cut(s) 266, 615, 1016, 2939
Psp1406I AACGTT 2 cut(s) 269, 2192
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 2 cut(s) 1115, 1332
PspEI GGTNACC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 1115, 1332
PspN4I GGNNCC 2 cut(s) 204, 2987
PspPI GGNCC 4 cut(s) 203, 1194, 1330, 3117
PspPPI RGGWCCY 1 cut(s) 203
PstI CTGCAG 1 cut(s) 845
PstNI CAGNNNCTG 1 cut(s) 1921
PsuI RGATCY 5 cut(s) 286, 588, 1896, 1942, 2985
PvuI CGATCG 2 cut(s) 244, 1542
PvuII CAGCTG 2 cut(s) 674, 1355
RsaI GTAC 9 cut(s) 165, 669, 853, 916, 1535, 1613, 2128, 2592, 2839
RsaNI GTAC 9 cut(s) 164, 668, 852, 915, 1534, 1612, 2127, 2591, 2838
RseI CAYNNNNRTG 1 cut(s) 2761
SapI GCTCTTC 3 cut(s) 961, 2864, 2994
SatI GCNGC 9 cut(s) 567, 796, 831, 1353, 1763, 1786, 2026, 2490, 2719
Sau96I GGNCC 4 cut(s) 203, 1194, 1330, 3117
ScaI AGTACT 1 cut(s) 165
SchI GAGTC 4 cut(s) 52, 347, 613, 3164
ScrFI CCNGG 3 cut(s) 1117, 1334, 1682
SduI GDGCHC 1 cut(s) 1318
SfaNI GCATC 4 cut(s) 146, 667, 1552, 1656
SfcI CTRYAG 1 cut(s) 841
SfuI TTCGAA 1 cut(s) 498
SinI GGWCC 3 cut(s) 203, 1194, 1330
SmiMI CAYNNNNRTG 1 cut(s) 2761
SmlI CTYRAG 2 cut(s) 60, 571
SmoI CTYRAG 2 cut(s) 60, 571
SpeI ACTAGT 1 cut(s) 1955
SphI GCATGC 1 cut(s) 2766
SseBI AGGCCT 1 cut(s) 131
SsiI CCGC 7 cut(s) 41, 658, 795, 1763, 1770, 2025, 2581
SspMI CTAG 4 cut(s) 1364, 1626, 1956, 2391
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 3 cut(s) 1115, 1332, 1680
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 5 cut(s) 88, 1603, 2549, 2617, 2970
TaiI ACGT 2 cut(s) 272, 2195
TaqI TCGA 9 cut(s) 9, 18, 244, 341, 478, 498, 1558, 2167, 2329
TatI WGTACW 1 cut(s) 163
TauI GCSGC 3 cut(s) 798, 1765, 2028
TscAI CASTG 3 cut(s) 1608, 1795, 2554
TseFI GTSAC 2 cut(s) 49, 2503
TseI GCWGC 6 cut(s) 566, 830, 1352, 1785, 2489, 2718
Tsp45I GTSAC 2 cut(s) 49, 2503
TspGWI ACGGA 2 cut(s) 1954, 2035
TspRI CASTG 3 cut(s) 1608, 1795, 2554
Van91I CCANNNNNTGG 1 cut(s) 1573
Vha464I CTTAAG 1 cut(s) 571
VpaK11BI GGWCC 3 cut(s) 203, 1194, 1330
VspI ATTAAT 1 cut(s) 2033
XapI RAATTY 7 cut(s) 438, 686, 941, 1815, 2073, 2361, 2864
XceI RCATGY 4 cut(s) 827, 2740, 2750, 2766
XmiI GTMKAC 3 cut(s) 1285, 1426, 3208
XmnI GAANNNNTTC 5 cut(s) 494, 1222, 2417, 3003, 3145
XspI CTAG 4 cut(s) 1364, 1626, 1956, 2391
ZrmI AGTACT 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 1671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.