RchiOBHm_Chr5g0064091

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Forward (+)
69882221 .. 69883737
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34016

Sequence Viewer

Length: 1476 bp
ATGGAAGAGGTCACGAAGGCACTGAAAAATGATGACATCACTACCATTGGGGTTGAGGGCATGGGAGGGGTTGGCAAGACAAGCATGGTGAAATATGTTGCTGCACAAGCTCTAAAAAATGGGCATTTTAATCATGTGATTTTGGCTGTCATATCCCAAAGTCCCGACTTGTTAAGAATTCAAGGCATATTCGCAGACCTTCTGCACTTGAGATTGCATGAGGAGACATTGATTGGAAGAGCTGTTAAATTGAGAGCTAGGATGATGAGAGCAAACAAGATCCTCATAATCTTAGATGACATGTGGGACGAAATTGATTTGTCAAGCATAGGAGTTCCAAACTACAGCGAGCTTCAAAGATGCCATTCCAAAGTCATGCTGACCACAAGAAGAAGAAATGTATGTCACGTCATGAGAATGCAAGCACAAATTACTCTAGATATCCTTTCCGAAGAAGATTCTTGGACTTTGTTTGTAAAAAATGCAGCATCTTCTTTTCAGAAATCAAATGCAGCATCATCTTTTCAGGAATCAACCATTTTCTATGATATAGCGAGAAAGGTAGCTAGGGAATGTGCTGGTCTACCAATTGCATTGATAGCAGTTTCAAAAGCCCTTGGAGATAAAGACATAGAAGAATGGAAAGAAGCAGCTCAACGCCTAGAGAGGTCCCAAACAGCCAATCTCGATGACAAGGGAGATGTATTCAAATGCATAAAGTTCAGCTATGATTACTTGAGATCTAATGATGCCAAGTCATGTTTCTTGCTTTGCTGCCTATTCCCAGAAGATGAAGATATCCAAATAACAGACTTGATGAAATATGGGCTCGGGAAAGGATTGTTTACAGATGCCAACACACTAGAAGAAGCTCGAGGTATAGCACATTCAGTGGTCAAGTACCTTAAAGCTTCTTGCTTACTTTTGGATAGTACTGAGGATGGGTGCGTAAGGATGCATGATGTCATCCGGGATACAGCCATGTCAATTGCATTGTCTGAAGATGGCCATGGTTTTTTGGTAAAAGCTGGCTGTAAACTAAAGAATTGGCCAAGGGGTATGCATGAAGGCTACTCTGCAATCTCACTAATGGGAAACAAGATTCGCAAGCTTCCCGAGAATTTGGTATGTTCAAAACTCCAGATTTTATTACTACAGACTAATTATGATATACAAGAGATTCCAGAAAGCTTTTTCCAAAGTCCTAGTTCATTGATGGTCTTGGATTTTAGCAAAACTAGTATTTCATTACTACCCCAATCATTGAGTCTCCTAACCAACCTCCAAGCTTTGTATTTGGATTCTTGCAAGAAAATAATTGACATATCAATACTTGGAAACCTGAAGAAGCTTGAGATTCTTAGTATGAGAAAATTTCCTCTTATGGAATTGCCAGAAGAAATAGGGAATTTGGCTAATCTTAGGATGTTGGACGTCACTGGTGGATGTATTAGAAGAACTATACATGCAATGTGA

Protein Analysis

491

Amino Acids

54.98

Weight (kDa)

6.61

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 163 9.8e-29 NB-ARC domain
LRR_8 PF13855 379 - 436 1.3e-09 Leucine rich repeat
LRR_14 PF23598 388 - 479 6.5e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1437
AccI GTMKAC 1 cut(s) 583
AclWI GGATC 1 cut(s) 274
AcoI YGGCCR 2 cut(s) 1006, 1049
AcsI RAATTY 4 cut(s) 177, 1120, 1373, 1408
AcuI CTGAAG 2 cut(s) 1020, 1364
AcyI GRCGYC 1 cut(s) 1434
AfaI GTAC 2 cut(s) 902, 934
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 5 cut(s) 182, 356, 609, 709, 1134
AhlI ACTAGT 1 cut(s) 1238
AjiI CACGTC 1 cut(s) 409
AjuI GAANNNNNNNTTGG 2 cut(s) 746, 778
Alw26I GTCTC 2 cut(s) 218, 1274
AlwI GGATC 1 cut(s) 274
Ama87I CYCGRG 3 cut(s) 830, 873, 1115
AoxI GGCC 2 cut(s) 1006, 1049
ApeKI GCWGC 5 cut(s) 101, 485, 512, 650, 774
ApoI RAATTY 4 cut(s) 177, 1120, 1373, 1408
AspS9I GGNCC 1 cut(s) 669
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 1 cut(s) 100
AvaI CYCGRG 3 cut(s) 830, 873, 1115
AvaII GGWCC 1 cut(s) 669
BalI TGGCCA 2 cut(s) 1008, 1051
BanII GRGCYC 1 cut(s) 831
BbvI GCAGC 5 cut(s) 88, 497, 524, 662, 761
BccI CCATC 3 cut(s) 935, 998, 1210
BciVI GTATCC 1 cut(s) 967
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 2 cut(s) 218, 1274
BcuI ACTAGT 1 cut(s) 1238
BfaI CTAG 7 cut(s) 258, 437, 567, 662, 863, 1206, 1239
BfmI CTRYAG 2 cut(s) 343, 1154
BfuI GTATCC 1 cut(s) 967
BglII AGATCT 1 cut(s) 740
BisI GCNGC 5 cut(s) 102, 486, 513, 651, 775
BlsI GCNGC 5 cut(s) 103, 487, 514, 652, 776
BmcAI AGTACT 1 cut(s) 934
Bme1390I CCNGG 1 cut(s) 971
Bme18I GGWCC 1 cut(s) 669
BmeT110I CYCGRG 3 cut(s) 830, 873, 1115
BmgBI CACGTC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 669
BmiI GGNNCC 1 cut(s) 671
BmrFI CCNGG 1 cut(s) 971
BmsI GCATC 6 cut(s) 350, 497, 524, 739, 841, 945
BpmI CTGGAG 1 cut(s) 1124
BpuEI CTTGAG 3 cut(s) 229, 757, 1373
BpuMI CCSGG 1 cut(s) 971
BsaHI GRCGYC 1 cut(s) 1434
BsaJI CCNNGG 3 cut(s) 616, 1009, 1052
Bse1I ACTGG 1 cut(s) 1444
Bse3DI GCAATG 1 cut(s) 1476
BseDI CCNNGG 3 cut(s) 616, 1009, 1052
BseGI GGATG 6 cut(s) 267, 946, 960, 966, 1431, 1451
BseMI GCAATG 1 cut(s) 1476
BseMII CTCAG 1 cut(s) 927
BseNI ACTGG 1 cut(s) 1444
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 5 cut(s) 88, 497, 524, 662, 761
BsgI GTGCAG 2 cut(s) 87, 188
BshFI GGCC 2 cut(s) 1008, 1051
BsiHKCI CYCGRG 3 cut(s) 830, 873, 1115
BsiSI CCGG 1 cut(s) 970
BslFI GGGAC 3 cut(s) 147, 320, 655
BsmAI GTCTC 2 cut(s) 218, 1274
BsmFI GGGAC 3 cut(s) 147, 320, 655
BsmI GAATGC 1 cut(s) 423
BsnI GGCC 2 cut(s) 1008, 1051
BsoBI CYCGRG 3 cut(s) 830, 873, 1115
Bsp1286I GDGCHC 1 cut(s) 831
Bsp143I GATC 2 cut(s) 279, 740
Bsp19I CCATGG 1 cut(s) 1009
BspANI GGCC 2 cut(s) 1008, 1051
BspCNI CTCAG 1 cut(s) 928
BspHI TCATGA 1 cut(s) 411
BspLI GGNNCC 1 cut(s) 671
BspPI GGATC 1 cut(s) 274
BspQI GCTCTTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 1476
BsrI ACTGG 1 cut(s) 1444
BssECI CCNNGG 3 cut(s) 616, 1009, 1052
BssMI GATC 2 cut(s) 279, 740
BssNI GRCGYC 1 cut(s) 1434
BssT1I CCWWGG 3 cut(s) 616, 1009, 1052
Bst6I CTCTTC 1 cut(s) 232
BstACI GRCGYC 1 cut(s) 1434
BstC8I GCNNGC 4 cut(s) 350, 423, 1030, 1109
BstDEI CTNAG 4 cut(s) 292, 936, 1361, 1421
BstDSI CCRYGG 1 cut(s) 1009
BstF5I GGATG 6 cut(s) 267, 946, 960, 966, 1431, 1451
BstKTI GATC 2 cut(s) 282, 743
BstMAI GTCTC 2 cut(s) 218, 1274
BstMBI GATC 2 cut(s) 279, 740
BstMWI GCNNNNNNNGC 3 cut(s) 81, 107, 599
BstNSI RCATGY 2 cut(s) 304, 1469
BstSCI CCNGG 1 cut(s) 969
BstSFI CTRYAG 2 cut(s) 343, 1154
BstV1I GCAGC 5 cut(s) 88, 497, 524, 662, 761
BstX2I RGATCY 2 cut(s) 279, 740
BstYI RGATCY 2 cut(s) 279, 740
BsuI GTATCC 1 cut(s) 967
BsuRI GGCC 2 cut(s) 1008, 1051
BtgI CCRYGG 1 cut(s) 1009
BtrI CACGTC 1 cut(s) 409
BtsCI GGATG 6 cut(s) 267, 946, 960, 966, 1431, 1451
BtsIMutI CAGTG 3 cut(s) 20, 897, 1437
Cac8I GCNNGC 4 cut(s) 350, 423, 1030, 1109
CciI TCATGA 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 669
Csp6I GTAC 2 cut(s) 901, 933
CviQI GTAC 2 cut(s) 901, 933
DdeI CTNAG 4 cut(s) 292, 936, 1361, 1421
DpnI GATC 2 cut(s) 281, 742
DpnII GATC 2 cut(s) 279, 740
EaeI YGGCCR 2 cut(s) 1006, 1049
Eam1104I CTCTTC 1 cut(s) 232
EarI CTCTTC 1 cut(s) 232
Eco130I CCWWGG 3 cut(s) 616, 1009, 1052
Eco24I GRGCYC 1 cut(s) 831
Eco32I GATATC 2 cut(s) 442, 799
Eco47I GGWCC 1 cut(s) 669
Eco57I CTGAAG 2 cut(s) 1020, 1364
Eco88I CYCGRG 3 cut(s) 830, 873, 1115
EcoO109I RGGNCCY 1 cut(s) 669
EcoRI GAATTC 1 cut(s) 177
EcoRV GATATC 2 cut(s) 442, 799
EcoT14I CCWWGG 3 cut(s) 616, 1009, 1052
EcoT22I ATGCAT 3 cut(s) 716, 960, 1065
EcoT38I GRGCYC 1 cut(s) 831
ErhI CCWWGG 3 cut(s) 616, 1009, 1052
FaqI GGGAC 3 cut(s) 147, 320, 655
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 5 cut(s) 102, 486, 513, 651, 775
FokI GGATG 6 cut(s) 274, 953, 953, 967, 1438, 1458
FriOI GRGCYC 1 cut(s) 831
Fsp4HI GCNGC 5 cut(s) 102, 486, 513, 651, 775
FspBI CTAG 7 cut(s) 258, 437, 567, 662, 863, 1206, 1239
GluI GCNGC 5 cut(s) 102, 486, 513, 651, 775
GsuI CTGGAG 1 cut(s) 1124
HaeIII GGCC 2 cut(s) 1008, 1051
HapII CCGG 1 cut(s) 970
Hin1I GRCGYC 1 cut(s) 1434
HindIII AAGCTT 5 cut(s) 909, 1109, 1189, 1287, 1349
HinfI GANTC 7 cut(s) 458, 530, 1102, 1180, 1267, 1301, 1357
HpaII CCGG 1 cut(s) 970
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 3 cut(s) 584, 846, 1037
Hpy188I TCNGA 3 cut(s) 451, 501, 1000
Hpy8I GTNNAC 3 cut(s) 584, 846, 1037
HpyAV CCTTC 3 cut(s) 10, 209, 1061
HpyCH4IV ACGT 2 cut(s) 408, 1434
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 107, 599
HpyF3I CTNAG 4 cut(s) 292, 936, 1361, 1421
HpySE526I ACGT 2 cut(s) 408, 1434
Hsp92I GRCGYC 1 cut(s) 1434
Kzo9I GATC 2 cut(s) 279, 740
LguI GCTCTTC 1 cut(s) 232
Lsp1109I GCAGC 5 cut(s) 88, 497, 524, 662, 761
LweI GCATC 6 cut(s) 350, 497, 524, 739, 841, 945
MaeI CTAG 7 cut(s) 258, 437, 567, 662, 863, 1206, 1239
MaeII ACGT 2 cut(s) 408, 1434
MaeIII GTNAC 3 cut(s) 10, 404, 1435
MalI GATC 2 cut(s) 281, 742
MboI GATC 2 cut(s) 279, 740
MfeI CAATTG 2 cut(s) 588, 987
MflI RGATCY 2 cut(s) 279, 740
MhlI GDGCHC 1 cut(s) 831
MlsI TGGCCA 2 cut(s) 1008, 1051
MluNI TGGCCA 2 cut(s) 1008, 1051
MlyI GAGTC 1 cut(s) 1276
MmeI TCCRAC 1 cut(s) 1410
MnlI CCTC 9 cut(s) 49, 59, 214, 293, 660, 869, 931, 1292, 1389
Mox20I TGGCCA 2 cut(s) 1008, 1051
Mph1103I ATGCAT 3 cut(s) 716, 960, 1065
MscI TGGCCA 2 cut(s) 1008, 1051
MseI TTAA 4 cut(s) 129, 173, 246, 906
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 2 cut(s) 1008, 1051
MspI CCGG 1 cut(s) 970
MspR9I CCNGG 1 cut(s) 971
MunI CAATTG 2 cut(s) 588, 987
Mva1269I GAATGC 1 cut(s) 423
MwoI GCNNNNNNNGC 3 cut(s) 81, 107, 599
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 1009
NdeII GATC 2 cut(s) 279, 740
NlaIV GGNNCC 1 cut(s) 671
NmuCI GTSAC 3 cut(s) 10, 404, 1435
NsiI ATGCAT 3 cut(s) 716, 960, 1065
NspI RCATGY 2 cut(s) 304, 1469
PaeR7I CTCGAG 1 cut(s) 873
PagI TCATGA 1 cut(s) 411
PciI ACATGT 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 232
PctI GAATGC 1 cut(s) 423
PfeI GAWTC 6 cut(s) 458, 530, 1102, 1180, 1301, 1357
PfoI TCCNGGA 1 cut(s) 969
PkrI GCNGC 5 cut(s) 103, 487, 514, 652, 776
PleI GAGTC 1 cut(s) 1275
PpsI GAGTC 1 cut(s) 1275
PpuMI RGGWCCY 1 cut(s) 669
PscI ACATGT 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 669
PspN4I GGNNCC 1 cut(s) 671
PspPI GGNCC 1 cut(s) 669
PspPPI RGGWCCY 1 cut(s) 669
PspXI VCTCGAGB 1 cut(s) 873
PsuI RGATCY 2 cut(s) 279, 740
RsaI GTAC 2 cut(s) 902, 934
RsaNI GTAC 2 cut(s) 901, 933
RseI CAYNNNNRTG 1 cut(s) 416
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 4 cut(s) 129, 173, 246, 906
SatI GCNGC 5 cut(s) 102, 486, 513, 651, 775
Sau3AI GATC 2 cut(s) 279, 740
Sau96I GGNCC 1 cut(s) 669
ScaI AGTACT 1 cut(s) 934
SchI GAGTC 1 cut(s) 1276
ScrFI CCNGG 1 cut(s) 971
SduI GDGCHC 1 cut(s) 831
SfaNI GCATC 6 cut(s) 350, 497, 524, 739, 841, 945
SfcI CTRYAG 2 cut(s) 343, 1154
Sfr274I CTCGAG 1 cut(s) 873
SinI GGWCC 1 cut(s) 669
SlaI CTCGAG 1 cut(s) 873
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 4 cut(s) 208, 736, 873, 1352
SmoI CTYRAG 4 cut(s) 208, 736, 873, 1352
SpeI ACTAGT 1 cut(s) 1238
SspMI CTAG 7 cut(s) 258, 437, 567, 662, 863, 1206, 1239
StyD4I CCNGG 1 cut(s) 969
StyI CCWWGG 3 cut(s) 616, 1009, 1052
TaiI ACGT 2 cut(s) 411, 1437
TaqI TCGA 2 cut(s) 687, 874
TatI WGTACW 1 cut(s) 932
TfiI GAWTC 6 cut(s) 458, 530, 1102, 1180, 1301, 1357
Tru1I TTAA 4 cut(s) 129, 173, 246, 906
Tru9I TTAA 4 cut(s) 129, 173, 246, 906
TscAI CASTG 3 cut(s) 27, 897, 1444
TseFI GTSAC 3 cut(s) 10, 404, 1435
TseI GCWGC 5 cut(s) 101, 485, 512, 650, 774
Tsp45I GTSAC 3 cut(s) 10, 404, 1435
TspDTI ATGAA 5 cut(s) 807, 833, 1080, 1200, 1236
TspRI CASTG 3 cut(s) 27, 897, 1444
VpaK11BI GGWCC 1 cut(s) 669
XapI RAATTY 4 cut(s) 177, 1120, 1373, 1408
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 2 cut(s) 304, 1469
XhoI CTCGAG 1 cut(s) 873
XmiI GTMKAC 1 cut(s) 583
XspI CTAG 7 cut(s) 258, 437, 567, 662, 863, 1206, 1239
ZraI GACGTC 1 cut(s) 1435
ZrmI AGTACT 1 cut(s) 934
Zsp2I ATGCAT 3 cut(s) 716, 960, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.