FvH4_3g43940

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Reverse (-)
36618650 .. 36622713
4064 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43940.t1

Sequence Viewer

Length: 3444 bp
ATGTTTGCATATTACAAAAAGATATCGTTGGCGATTGTTGCGGTAATTGTGACATGGATGGGTTGCAAGGCCATTAAGGGGATATGGACTCAAGTAAAGCGTCGGATAAGTTACATGGTCGGAAAGTCGCAGGATAATCAAGAAGTGATTAACTTGAATGCCTCTGAATCTCTTGTTTCATCATCAGAACCTCGTCGCTCCAAGTATGATGTGTTTTTGAGTTTCAGGGGTCTTGACACTCGGAGGGGAATTACTTCTCAATTATATGATCAGCTGCATAACAAGAGAAGAATTGCAACATTCATGGATGATCGAGATCTTGAAGTAGGGGATGCTATATCTCCCACTCTACTAAAAGCAATTGAAGAATCAAGGTTTGCAATTGTTGTCCTTTCTCCAAACTATGCCTCTTCTACTTGGTGTTTGGAGGAACTTGCGAAGATCTGTGAGTGCATGAAAGACCAGAACAGAATTCTTCCACTTTTCTATAATGTCGAGCCTACTAATGTACGGTTCCAGAAGAGTAGTTTCGAAGAAGCTTTCAACAAGCATGAAAGCTCTGGGCGATACACATCAGAGAAGGTGCAGCGGTGGAAGGATGCTTTGAGCAAAGTGGCCAGTTTCTCTGGGTGGGATTCAAACAATTACATAACTGATACAGAACTTGTGGAGAAAATTGTGGATTCTGTGTTCAAAAGAATACAACCTTTTGACGTTGAGTTTACAATGTCCACCGGAGATTTTGAAGCGTATGAAGCAACAAGAGAATCCATGGGTGAGATTATGAAGAAGCTTAAAGATGACAACGTCACTACCATTGGGGTCTACGGAATGGGCGGAGTCGGCAAGACAACCCTGGTAAAACATGTTGCTGCTCAATCGTGCAAAAATGGGATTTTTCATCATGTGGTTATGGCTGTTGTATCCCAAAGCCCCAACGTTGAAAGAATTCAACACACATTACTAGAAGAGCTGGTGGGCTTCTCTGAACTGGAGAATTTAACAGAAAGTGGAAGAGCCTCGAGATTGTATAAAGAGATAATGAGGAAAGACAATATTCTCATAATCTTGGACGACATTTGGGATTCTATAGATTTGTCCAGAATAGGAATTCCCAGCTATGACCTGCTCAAAACACACAATTCCAAAGTCCTAATCACCACGAGAAAATTGCATGTTTGTAATTCCATGCACAGCCAAGCAAGCATTCCTCTCAATATCTTATCAGAACAAGATTCTTGGAGCTTGTTTGTGAAGAAAACAAAAAGATCTTTTGAATCAACCACTTTTGAGGGTGTAGCGAGAAAGGTTGCTGGAGAATGTAGGGGTCTACCCATTGCTCTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGATCTGGCGGAATGGCAAACAGCAGCTCAACAATTAAAGAAGTCGCAAATTGCCAACCTTGACCATAAGGACGATGCTTTCAGTTGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAATCATGCTTCTTGTTGTGTTGCCTATTTCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCAAGTATGCAATTGGGAAAGGATTGTTTCGAGACACTGACACAATATATGAAGCCAGAGCAATAGCAGATACAGTGGTCAAGCACCTGATAGATTCAAGCTTGCTTTTGGATGGTAAATATCGTGGATGTGTACGGATGCACGGTGTCGTCCGGGATACAGCCATGAGAATTGCAAAATGTGAAGATGGGCATGGGTTTTTGGTGAGAGCTGGATGTGGTCTTAAGGATTGGTCATGTCGATCACATGAAGACTACTATGCTATCTCACTGATGGAGAATAAACTTCACAGTCTGCCCGATGAGTTGATATGTCCAAATCTTCAGATTCTGTTACTACAAGAGAATCAGGATTTGTATAAGATCCCAGAGACCTTTTTCCAAAGTCCGAATGAATTAAGGGTCTTAGATCTTAGCCGCACCAACATTTCATTGCTGCCCCAGTCATTCAATCTCCTGACCAAGCTCCAAGCTCTGCATCTAGATTTTTGCCGTAAAGGAGTTGACATTTCCATAGTCGGAAAACTTAAGAAGCTTGAGATACTTAGTATGAGAGATTATCCTCTTACGGAGCTGTCTAGAGAAATAAGAAATTTGACCAATCTAAGGATGTTGGATATCGGTGGCCGTAGTTCTCACGGTGGAGGTATTGTCACAATTCCATCTAAAGTGATATCAAAGCTGCATAGATTAGAAGAATTGTACATGATGTACTGTGGATTTGTGAACTGGGAAAGTCCAATTGAGGGAGAAGGAGATGAAATCAATATTGGCTTTGGTGAGCTAGCTGGTTTATCAAAGTTGAAAAATTTGCAGGTTTCCTTACCTAATGCAGAATGCATCCCTAAAAATGTCGAGGTCGAACCAGGTTGGTTTTACTTTGATATACGTATTGGTGGATGCGCAAGCGCAAGCGATCTAAATTATCAGTTTGATCGTAAATCAAGATCCTTGCTTCTTCGTGAAATAACCATCAGTACCTTACCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAAAAGCTAAAGTATGACCGCTGCAAAGGGATGAGTGACATTGTTATGGAATATGACCACGGGAGGTTACATAAACTCAAGCATCTCACAGTACGTACGGATTTCTATGGATACTTGAAAGTGTTGATGAACACAACAAGACGAGTTGAAACAGGACCCGTGTTTGAGAATTTGGTAGAGTTGCATCTGATACATCTGATTCGCCTGGAGGAGTTATGTGTTGGTGAGCTACCACCTGGGTCTCTCTCAAATCTCAAGGTATTGCATATGCATGGTTGTTTTATCTTGAAGAGTGTATCAAAATTTGTACATAGACTACCAAATCTGGAGAAACTATATCTGAATCGGATGAAGGAAATGGAATATGTGTTTGGATGCGAAGGGTGTGAGCCAGAACAATCAAAACTGAAAGAGATGCATTTGTTGTATCTAAAAGCACTAAGAAGCATATGTAACGGTCCGGCTCCACGTGCAATGTTTCGGAGTCTTAAAACCTTGACCGTGTACCATTGCGAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTGAGTGTCTTGTTCAATTGGAGGACCTTCTTGTAGAGGATTGTCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAAGATAAGACGGTTCTACCAAACCTGAAGAACTTGGTTTTGAAGAATCTTCCTATGTTGTATGGTCCAAGTGCTACTACTGTTGATATTGAGTGTCCTTCATTGGAACGATTGGTGGTGGTGGATTGCCCCCAGCTTCCATTTTCAACCTCTTCCGATCTCTTCCTACTCTACTTTGGCAGCAGGAACCCTGTCCAACTCAATGATCCGCAACTGTACAAGTTTTTACGTAGCTGA

Protein Analysis

1148

Amino Acids

130.64

Weight (kDa)

6.58

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 68 - 237 1.2e-49 TIR domain
TIR_2 PF13676 71 - 167 7.5e-15 TIR domain
NB-ARC PF00931 256 - 421 8.3e-33 NB-ARC domain
LRR_8 PF13855 627 - 685 6.6e-09 Leucine rich repeat
LRR_14 PF23598 652 - 760 6.1e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 920 - 1058 3.8e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2467
Acc36I ACCTGC 2 cut(s) 1134, 2368
AccI GTMKAC 2 cut(s) 825, 1330
AciI CCGC 8 cut(s) 41, 589, 837, 1382, 1981, 2567, 2602, 3416
AclI AACGTT 1 cut(s) 939
AclWI GGATC 3 cut(s) 1921, 2505, 3407
AcoI YGGCCR 2 cut(s) 615, 2188
AcsI RAATTY 8 cut(s) 471, 948, 997, 1110, 2155, 2371, 2752, 2885
AcuI CTGAAG 2 cut(s) 1871, 3254
AcvI CACGTG 1 cut(s) 3053
AfiI CCNNNNNNNGG 3 cut(s) 78, 2169, 2309
AflII CTTAAG 2 cut(s) 1787, 2090
AflIII ACRYGT 1 cut(s) 865
AjnI CCWGG 4 cut(s) 855, 2428, 2787, 2818
AjuI GAANNNNNNNTTGG 6 cut(s) 1191, 1223, 2316, 2348, 2937, 2969
AloI GAACNNNNNNTCC 2 cut(s) 674, 706
Alw26I GTCTC 3 cut(s) 1590, 1928, 2829
AlwI GGATC 3 cut(s) 1921, 2505, 3407
AlwNI CAGNNNCTG 1 cut(s) 1894
Ama87I CYCGRG 1 cut(s) 1021
AoxI GGCC 3 cut(s) 69, 615, 2188
ApoI RAATTY 8 cut(s) 471, 948, 997, 1110, 2155, 2371, 2752, 2885
Asp700I GAANNNNTTC 2 cut(s) 253, 948
AspLEI GCGC 2 cut(s) 2468, 2474
AspS9I GGNCC 4 cut(s) 2738, 3041, 3154, 3272
AsuC2I CCSGG 1 cut(s) 1718
AsuHPI GGTGA 6 cut(s) 788, 1150, 1780, 2354, 2581, 2819
AsuII TTCGAA 1 cut(s) 531
AsuNHI GCTAGC 1 cut(s) 2347
AvaI CYCGRG 1 cut(s) 1021
AvaII GGWCC 4 cut(s) 2738, 3041, 3154, 3272
BaeGI GKGCMC 1 cut(s) 1363
BaeI ACNNNNGTAYC 2 cut(s) 1626, 1659
BalI TGGCCA 1 cut(s) 617
BauI CACGAG 1 cut(s) 1162
BbrPI CACGTG 1 cut(s) 3053
BbsI GAAGAC 3 cut(s) 1542, 1563, 1821
BccI CCATC 6 cut(s) 52, 1670, 1745, 1831, 2233, 2543
BceAI ACGGC 2 cut(s) 2040, 2175
BcgI CGANNNNNNTGC 2 cut(s) 1153, 1187
BciT130I CCWGG 4 cut(s) 857, 2430, 2789, 2820
BciVI GTATCC 3 cut(s) 934, 1714, 2687
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 1 cut(s) 1718
BcoDI GTCTC 3 cut(s) 1590, 1928, 2829
BfaI CTAG 4 cut(s) 965, 2045, 2142, 2348
BfmI CTRYAG 1 cut(s) 1089
BfrI CTTAAG 2 cut(s) 1787, 2090
BfuAI ACCTGC 2 cut(s) 1134, 2368
BfuI GTATCC 3 cut(s) 934, 1714, 2687
BglII AGATCT 5 cut(s) 316, 441, 1268, 1375, 1972
Bme1390I CCNGG 5 cut(s) 857, 1718, 2430, 2789, 2820
Bme18I GGWCC 4 cut(s) 2738, 3041, 3154, 3272
BmeT110I CYCGRG 1 cut(s) 1021
BmgT120I GGNCC 4 cut(s) 2738, 3041, 3154, 3272
BmiI GGNNCC 4 cut(s) 515, 2740, 3048, 3395
BmrFI CCNGG 5 cut(s) 857, 1718, 2430, 2789, 2820
BmrI ACTGGG 2 cut(s) 1999, 2302
BmtI GCTAGC 1 cut(s) 2351
BmuI ACTGGG 2 cut(s) 1999, 2302
BpiI GAAGAC 3 cut(s) 1542, 1563, 1821
BpmI CTGGAG 4 cut(s) 1013, 1335, 2810, 2930
Bpu14I TTCGAA 1 cut(s) 531
BpuEI CTTGAG 4 cut(s) 75, 2120, 2645, 2822
BpuMI CCSGG 1 cut(s) 1718
BsaAI YACGTR 4 cut(s) 2453, 2678, 3053, 3437
BsaBI GATNNNNATC 3 cut(s) 315, 571, 1374
BsaI GGTCTC 2 cut(s) 1928, 2829
BsaJI CCNNGG 4 cut(s) 771, 855, 2641, 2819
BsaWI WCCGGW 1 cut(s) 734
BsaXI ACNNNNNCTCC 2 cut(s) 2199, 2229
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 2169, 2309
Bse1I ACTGG 4 cut(s) 618, 996, 2005, 2297
Bse3DI GCAATG 4 cut(s) 1335, 1994, 3063, 3091
Bse8I GATNNNNATC 3 cut(s) 315, 571, 1374
BseBI CCWGG 4 cut(s) 857, 2430, 2789, 2820
BseDI CCNNGG 4 cut(s) 771, 855, 2641, 2819
BseJI GATNNNNATC 3 cut(s) 315, 571, 1374
BseLI CCNNNNNNNGG 3 cut(s) 78, 2169, 2309
BseMI GCAATG 4 cut(s) 1335, 1994, 3063, 3091
BseMII CTCAG 1 cut(s) 3123
BseNI ACTGG 4 cut(s) 618, 996, 2005, 2297
BseRI GAGGAG 1 cut(s) 2807
BseSI GKGCMC 1 cut(s) 1363
BseYI CCCAGC 2 cut(s) 1115, 3339
BsgI GTGCAG 1 cut(s) 605
BshFI GGCC 3 cut(s) 71, 617, 2190
BsiHKCI CYCGRG 1 cut(s) 1021
BsiSI CCGG 3 cut(s) 735, 1717, 3044
BsiWI CGTACG 1 cut(s) 2678
BslI CCNNNNNNNGG 3 cut(s) 78, 2169, 2309
BsmAI GTCTC 3 cut(s) 1590, 1928, 2829
BsmI GAATGC 3 cut(s) 163, 1206, 2405
BsnI GGCC 3 cut(s) 71, 617, 2190
Bso31I GGTCTC 2 cut(s) 1928, 2829
BsoBI CYCGRG 1 cut(s) 1021
Bsp119I TTCGAA 1 cut(s) 531
Bsp1286I GDGCHC 1 cut(s) 1363
Bsp1407I TGTACA 3 cut(s) 2265, 2890, 3423
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 8 cut(s) 41, 589, 837, 1382, 1981, 2567, 2602, 3416
BspANI GGCC 3 cut(s) 71, 617, 2190
BspCNI CTCAG 1 cut(s) 3124
BspLI GGNNCC 4 cut(s) 515, 2740, 3048, 3395
BspMI ACCTGC 2 cut(s) 1134, 2368
BspOI GCTAGC 1 cut(s) 2351
BspPI GGATC 3 cut(s) 1921, 2505, 3407
BspQI GCTCTTC 2 cut(s) 963, 1009
BspT104I TTCGAA 1 cut(s) 531
BspTI CTTAAG 2 cut(s) 1787, 2090
BspTNI GGTCTC 2 cut(s) 1928, 2829
BsrDI GCAATG 4 cut(s) 1335, 1994, 3063, 3091
BsrGI TGTACA 3 cut(s) 2265, 2890, 3423
BsrI ACTGG 4 cut(s) 618, 996, 2005, 2297
BssECI CCNNGG 4 cut(s) 771, 855, 2641, 2819
BssSI CACGAG 1 cut(s) 1162
BssT1I CCWWGG 1 cut(s) 771
Bst2BI CACGAG 1 cut(s) 1162
Bst2UI CCWGG 4 cut(s) 857, 2430, 2789, 2820
Bst6I CTCTTC 7 cut(s) 415, 515, 963, 1009, 2867, 3364, 3374
BstAFI CTTAAG 2 cut(s) 1787, 2090
BstAUI TGTACA 3 cut(s) 2265, 2890, 3423
BstBAI YACGTR 4 cut(s) 2453, 2678, 3053, 3437
BstBI TTCGAA 1 cut(s) 531
BstC8I GCNNGC 6 cut(s) 1204, 1667, 2349, 2470, 2476, 3098
BstDEI CTNAG 6 cut(s) 1969, 1976, 2108, 2168, 3023, 3132
BstDSI CCRYGG 2 cut(s) 771, 2641
BstHHI GCGC 2 cut(s) 2468, 2474
BstMAI GTCTC 3 cut(s) 1590, 1928, 2829
BstMWI GCNNNNNNNGC 6 cut(s) 38, 755, 843, 1203, 1346, 3053
BstNI CCWGG 4 cut(s) 857, 2430, 2789, 2820
BstNSI RCATGY 2 cut(s) 869, 1178
BstSCI CCNGG 5 cut(s) 855, 1716, 2428, 2787, 2818
BstSFI CTRYAG 1 cut(s) 1089
BstSLI GKGCMC 1 cut(s) 1363
BstSNI TACGTA 3 cut(s) 2453, 2678, 3437
BstV2I GAAGAC 3 cut(s) 1542, 1563, 1821
BstX2I RGATCY 7 cut(s) 316, 441, 1268, 1375, 1926, 1972, 2510
BstYI RGATCY 7 cut(s) 316, 441, 1268, 1375, 1926, 1972, 2510
BsuI GTATCC 3 cut(s) 934, 1714, 2687
BsuRI GGCC 3 cut(s) 71, 617, 2190
BtgI CCRYGG 2 cut(s) 771, 2641
BtsI GCAGTG 1 cut(s) 3207
BtsIMutI CAGTG 4 cut(s) 1599, 1644, 1832, 3207
BveI ACCTGC 2 cut(s) 1134, 2368
Cac8I GCNNGC 6 cut(s) 1204, 1667, 2349, 2470, 2476, 3098
CaiI CAGNNNCTG 1 cut(s) 1894
CfoI GCGC 2 cut(s) 2468, 2474
Cfr13I GGNCC 4 cut(s) 2738, 3041, 3154, 3272
CpoI CGGWCCG 1 cut(s) 3041
CseI GACGC 1 cut(s) 89
CsiI ACCWGGT 1 cut(s) 2428
CspI CGGWCCG 1 cut(s) 3041
DdeI CTNAG 6 cut(s) 1969, 1976, 2108, 2168, 3023, 3132
EaeI YGGCCR 2 cut(s) 615, 2188
Eam1104I CTCTTC 7 cut(s) 415, 515, 963, 1009, 2867, 3364, 3374
EarI CTCTTC 7 cut(s) 415, 515, 963, 1009, 2867, 3364, 3374
EciI GGCGGA 2 cut(s) 852, 1397
Eco105I TACGTA 3 cut(s) 2453, 2678, 3437
Eco130I CCWWGG 1 cut(s) 771
Eco31I GGTCTC 2 cut(s) 1928, 2829
Eco32I GATATC 3 cut(s) 24, 2182, 2238
Eco47I GGWCC 4 cut(s) 2738, 3041, 3154, 3272
Eco57I CTGAAG 2 cut(s) 1871, 3254
Eco72I CACGTG 1 cut(s) 3053
Eco88I CYCGRG 1 cut(s) 1021
EcoO109I RGGNCCY 2 cut(s) 2738, 3154
EcoRI GAATTC 3 cut(s) 471, 948, 1110
EcoRII CCWGG 4 cut(s) 855, 2428, 2787, 2818
EcoRV GATATC 3 cut(s) 24, 2182, 2238
EcoT14I CCWWGG 1 cut(s) 771
EcoT22I ATGCAT 3 cut(s) 2405, 2856, 3003
ErhI CCWWGG 1 cut(s) 771
FauNDI CATATG 2 cut(s) 2850, 3032
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 2 cut(s) 825, 1330
FspBI CTAG 4 cut(s) 965, 2045, 2142, 2348
FspI TGCGCA 1 cut(s) 2467
GlaI GCGC 2 cut(s) 2467, 2473
GsaI CCCAGC 2 cut(s) 1119, 3343
GsuI CTGGAG 4 cut(s) 1013, 1335, 2810, 2930
HaeIII GGCC 3 cut(s) 71, 617, 2190
HapII CCGG 3 cut(s) 735, 1717, 3044
HgaI GACGC 1 cut(s) 89
HhaI GCGC 2 cut(s) 2468, 2474
Hin6I GCGC 2 cut(s) 2466, 2472
HinP1I GCGC 2 cut(s) 2466, 2472
HincII GTYRAC 1 cut(s) 2068
HindII GTYRAC 1 cut(s) 2068
HindIII AAGCTT 4 cut(s) 537, 791, 1663, 2096
HpaII CCGG 3 cut(s) 735, 1717, 3044
HphI GGTGA 6 cut(s) 788, 1150, 1780, 2354, 2581, 2819
Hpy166II GTNNAC 9 cut(s) 723, 732, 826, 1331, 1697, 2068, 2290, 3088, 3205
Hpy8I GTNNAC 9 cut(s) 723, 732, 826, 1331, 1697, 2068, 2290, 3088, 3205
Hpy99I CGWCG 2 cut(s) 105, 198
HpyAV CCTTC 7 cut(s) 574, 589, 2309, 2929, 2957, 3167, 3315
HpyCH4IV ACGT 7 cut(s) 714, 807, 939, 2452, 2677, 3052, 3436
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 755, 843, 1203, 1346, 3053
HpyF3I CTNAG 6 cut(s) 1969, 1976, 2108, 2168, 3023, 3132
HpySE526I ACGT 7 cut(s) 714, 807, 939, 2452, 2677, 3052, 3436
HspAI GCGC 2 cut(s) 2466, 2472
Ksp22I TGATCA 1 cut(s) 268
LguI GCTCTTC 2 cut(s) 963, 1009
LmnI GCTCC 5 cut(s) 203, 1242, 2034, 2134, 3052
MabI ACCWGGT 1 cut(s) 2428
MaeI CTAG 4 cut(s) 965, 2045, 2142, 2348
MaeII ACGT 7 cut(s) 714, 807, 939, 2452, 2677, 3052, 3436
MaeIII GTNAC 9 cut(s) 49, 110, 808, 1896, 2215, 2569, 2618, 2649, 3035
MfeI CAATTG 5 cut(s) 360, 381, 1575, 2304, 3146
MflI RGATCY 7 cut(s) 316, 441, 1268, 1375, 1926, 1972, 2510
MhlI GDGCHC 1 cut(s) 1363
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 3 cut(s) 82, 849, 3076
MmeI TCCRAC 6 cut(s) 83, 100, 2062, 2157, 3162, 3427
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 3 cut(s) 2405, 2856, 3003
MroXI GAANNNNTTC 2 cut(s) 253, 948
MscI TGGCCA 1 cut(s) 617
MslI CAYNNNNRTG 2 cut(s) 2627, 2853
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 3 cut(s) 274, 589, 2604
MspCI CTTAAG 2 cut(s) 1787, 2090
MspI CCGG 3 cut(s) 735, 1717, 3044
MspR9I CCNGG 5 cut(s) 857, 1718, 2430, 2789, 2820
MunI CAATTG 5 cut(s) 360, 381, 1575, 2304, 3146
Mva1269I GAATGC 3 cut(s) 163, 1206, 2405
MvaI CCWGG 4 cut(s) 857, 2430, 2789, 2820
MwoI GCNNNNNNNGC 6 cut(s) 38, 755, 843, 1203, 1346, 3053
NciI CCSGG 1 cut(s) 1718
NcoI CCATGG 1 cut(s) 771
NdeI CATATG 2 cut(s) 2850, 3032
NheI GCTAGC 1 cut(s) 2347
NlaIV GGNNCC 4 cut(s) 515, 2740, 3048, 3395
NmuCI GTSAC 5 cut(s) 49, 808, 2215, 2569, 2618
NsbI TGCGCA 1 cut(s) 2467
NsiI ATGCAT 3 cut(s) 2405, 2856, 3003
NspI RCATGY 2 cut(s) 869, 1178
NspV TTCGAA 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 1021
PciI ACATGT 1 cut(s) 865
PciSI GCTCTTC 2 cut(s) 963, 1009
PctI GAATGC 3 cut(s) 163, 1206, 2405
PdmI GAANNNNTTC 2 cut(s) 253, 948
Pfl23II CGTACG 1 cut(s) 2678
PflFI GACNNNGTC 1 cut(s) 806
PfoI TCCNGGA 1 cut(s) 1716
PleI GAGTC 3 cut(s) 82, 848, 3075
PmaCI CACGTG 1 cut(s) 3053
PmlI CACGTG 1 cut(s) 3053
PpsI GAGTC 3 cut(s) 82, 848, 3075
Ppu21I YACGTR 4 cut(s) 2453, 2678, 3053, 3437
PpuMI RGGWCCY 2 cut(s) 2738, 3154
PscI ACATGT 1 cut(s) 865
Psp1406I AACGTT 1 cut(s) 939
Psp5II RGGWCCY 2 cut(s) 2738, 3154
Psp6I CCWGG 4 cut(s) 855, 2428, 2787, 2818
PspCI CACGTG 1 cut(s) 3053
PspFI CCCAGC 2 cut(s) 1115, 3339
PspGI CCWGG 4 cut(s) 855, 2428, 2787, 2818
PspLI CGTACG 1 cut(s) 2678
PspN4I GGNNCC 4 cut(s) 515, 2740, 3048, 3395
PspPI GGNCC 4 cut(s) 2738, 3041, 3154, 3272
PspPPI RGGWCCY 2 cut(s) 2738, 3154
PstNI CAGNNNCTG 1 cut(s) 1894
PsuI RGATCY 7 cut(s) 316, 441, 1268, 1375, 1926, 1972, 2510
PsyI GACNNNGTC 1 cut(s) 806
PvuII CAGCTG 1 cut(s) 274
RseI CAYNNNNRTG 2 cut(s) 2627, 2853
Rsr2I CGGWCCG 1 cut(s) 3041
RsrII CGGWCCG 1 cut(s) 3041
SapI GCTCTTC 2 cut(s) 963, 1009
Sau96I GGNCC 4 cut(s) 2738, 3041, 3154, 3272
SchI GAGTC 3 cut(s) 82, 849, 3076
ScrFI CCNGG 5 cut(s) 857, 1718, 2430, 2789, 2820
SduI GDGCHC 1 cut(s) 1363
SexAI ACCWGGT 1 cut(s) 2428
SfcI CTRYAG 1 cut(s) 1089
Sfr274I CTCGAG 1 cut(s) 1021
SfuI TTCGAA 1 cut(s) 531
SinI GGWCC 4 cut(s) 2738, 3041, 3154, 3272
SlaI CTCGAG 1 cut(s) 1021
SmiMI CAYNNNNRTG 2 cut(s) 2627, 2853
SmlI CTYRAG 7 cut(s) 90, 1021, 1787, 2090, 2099, 2660, 2837
SmoI CTYRAG 7 cut(s) 90, 1021, 1787, 2090, 2099, 2660, 2837
SnaBI TACGTA 3 cut(s) 2453, 2678, 3437
SsiI CCGC 8 cut(s) 41, 589, 837, 1382, 1981, 2567, 2602, 3416
SspI AATATT 2 cut(s) 1057, 2332
SspMI CTAG 4 cut(s) 965, 2045, 2142, 2348
StyD4I CCNGG 5 cut(s) 855, 1716, 2428, 2787, 2818
StyI CCWWGG 1 cut(s) 771
TaiI ACGT 7 cut(s) 717, 810, 942, 2455, 2680, 3055, 3439
TaqI TCGA 8 cut(s) 313, 495, 531, 1022, 1594, 1804, 2418, 2424
TatI WGTACW 4 cut(s) 2265, 2274, 2890, 3423
TauI GCSGC 1 cut(s) 1983
TscAI CASTG 4 cut(s) 1606, 1644, 1839, 3207
TseFI GTSAC 5 cut(s) 49, 808, 2215, 2569, 2618
Tsp45I GTSAC 5 cut(s) 49, 808, 2215, 2569, 2618
TspGWI ACGGA 4 cut(s) 843, 1714, 2147, 2696
TspRI CASTG 4 cut(s) 1606, 1644, 1839, 3207
Tth111I GACNNNGTC 1 cut(s) 806
Vha464I CTTAAG 2 cut(s) 1787, 2090
VpaK11BI GGWCC 4 cut(s) 2738, 3041, 3154, 3272
XapI RAATTY 8 cut(s) 471, 948, 997, 1110, 2155, 2371, 2752, 2885
XbaI TCTAGA 2 cut(s) 2044, 2141
XceI RCATGY 2 cut(s) 869, 1178
XhoI CTCGAG 1 cut(s) 1021
XmiI GTMKAC 2 cut(s) 825, 1330
XmnI GAANNNNTTC 2 cut(s) 253, 948
XspI CTAG 4 cut(s) 965, 2045, 2142, 2348
Zsp2I ATGCAT 3 cut(s) 2405, 2856, 3003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.