FvH4_1g28300

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000344
Physical Location & Seq
Reverse (-)
20118806 .. 20128863
10058 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28300.t2

Sequence Viewer

Length: 3339 bp
ATGGCCGCCGATCCAACTCAGCAGCAGCAGCAGCAGCAGCTGGCCGTGCTCCTGGGCCAGGACCCGGCTGAGTTCGAAACCCTAATATCACATCTGATGTCGTCGACGAACGAGCAGCGGTCCGAGGCCGAGAGGGTGTTCAACCTGTGCAAGCAGGCCCACCCAGACGCCCTGGTTCTGAAGCTGGTCCATGTCCTCCAGGCCGCGCCCCGACCCGACTCCCGCACCATGTCCGTGATCCTCCTCCGCCGCCAGCTCGACTCGTCCCTCTGGGCCCGCCTCTCCCCCTCCACCCACTCCTCCCTCAAGTCCGCTCTCCTCTCCTCCCTCCACTCCGAGTCCACCTCCAAATCCATGTCCAAGAAGCTCTGCGACACCGTCTCCGAGCTCGCTTCTTCTCTTCTCCCCGACAACCAGTGGCCGGACCTCTTGCCTTTCATGTTCCACTGCGTCTCCTCCGACAACCCCAAGCTCCAGGAGTCGGCTCTCTTCATTTTCGCCCACCTCGCTCATTACATCGCCTCCCAAGCCATGCTCCCCCAGCTCACCACCCTCCACTCCGTCTTCTACCGGTGCCTCTCCTCCGCCGACGTCCGAATCGCCGCCCTCACCGCCTCTGTCAATTTCATCCAGTGCCTCACCTCCCCCTCCCACAGGGACATGTTCCAGGACCTCTTGCCCTTGATGATGCAGACTTTGACAGAGGCCTTGAACTCCGGTCAGGAGGCCACCGCGCAGGAGGCCCTCGAGTTGCTTATCGAACTGGCCGGCACTGAGCCCCGGTTCCTGAGGAGGCAGCTGGTGGATGTGGTTGGCTCCATGCTTCAGATTGCCGAGGCATCCTCCCTGGAGGAAGGGACCCGGCATTTGGCCATTGAGTTTGTCATCACTCTGGCCGAGGCCAGGGAGAGAGCGCCCGGGATGATGAGGAAGCTGCCTCAGTTTATTCATAGGTTGTTTGCCATACTCATGACCATGTTGTTGGACCTTGACGACGACCCCGACTGGCACACTGCAGATACTGAGAATGAGGATGCTGGTGAGACTAGTAACTACGGCTTTGGACAGGAGTGTTTAGACAGGCTTTCAATTTCAGTGGGTGGCAATACCATTATACCTGTTGCTTCCGAGCTTTTTCCGTCCTACTTGGTTGCCCCTGAATGGCAAAAGCACCATGCCGTCCACATTGCCCTTGCTCAGATTGCCGAGGGCTGCTCTAAGGTTATGGTGCAGAACCTCGAACAGGTGGTGTCAACGGTATTGAATTCTTTCCAAGATCCTCACCCACGTGTGCGGTGGGCAGCAATTAATGCAGTTGGGCAATTGTCAACGGATTTAGGCCCAAATTTACAAGAGAAGTATCACCAGCGAGTGCTGCCTGCATTAGCTGGAGCTATGGATGACTTCCAAAATCCACGAGTACAGGCCCATGCCGCTTCAGCAGTACTCAATTTCAGTGAAAACTGCACCCCAGATATTTTGGTACCGTACCTGGATGGAATTGTGAGCAAGCTTCTTGTACTTCTACAGAATGGAAAGCAGATGGTCCAGGAGGGGGCTTTAACAGCTTTAGCATCAGTTGCTGATTCGTCACAGGAGCAATTCCAGAAGTATTATGATGCTGTAATGCCTTACTTGAAAGCCATTTTGGTTAATGCAAATGACAAAGCTAATCGTATGCTACGTGCAAAATCAATGGAATGTATTAGTTTGGTTGGAATGGCTGTCGGGAAGGATAAGTTTAGGGATGATGCAAAGCAGGTTATGGAAGTCTTGATGTCATTGCAAGGATCTGAAATGGAGGCAGATGATCCCACAACAAGTTATATGTTACAAGCATGGGCTAGACTTTGCAAATGCCTTGGGCAGGATTTTCTCCCATACATGAATGTTGTCATGCCTTCTTTGCTTCAGTCTGCTCAACTTAAGCCTGATGTGACAATCACTTCTGCAGATTCAGATGGAGAAATTGATGAAGATGATGATAGCATTGAAATGATTACTGTTGGGGATAAGAGGATAGGAATCAAAACTAGTGTTCTGGAGGAAAAAGCTACGGCCTGTAACATGCTATGTTGCTATGCTGATGAGTTGAAGGAAGGATTTTTCCCTTGGATTGATCAGGTTGCTCCAACATTGGTTCCTCTTCTAAAATTTTATTTCCATGAAGAAGTTAGAAAGGCAGCTGTTTCAGCAATGCCGGAGCTCCTACGTTCAGCCAAATTAGCAGTAGAGAAGGGCCAGTCTCAAGGCCATAATGAGTCCTACATAAAGCAGTTATCTGATTACATAATACCAGCGCTGGTTGAGGCATTGCACAAGGAACCTGAGGTTGAAATTTGTGCCAGCATTTTAGATGCATTGAACGAATGTGTGCAGAGTTCTGGGCCACTACTTGATGAAAACCAAGTTAGATGCATCGTGGATGAGATCAAACAAGTGATTACAGCAATCACATCTAGAAAACAAGAGCGAGCAGAAAGGGCCAAGGCAGAGGACTTTGATGCAGAAGAAGGGGAGCTTCTGAAGGAGGAAAATGAACAAGAAGAAGAGCTTTTTGATCTAGTTGGTGATTGTTTGGGAACTTTGATAAAGACATTCAAGGCTTCTTTTTTGCCTTTCTTTGACAAGCTTTCCTCTTACATAACACCAATGTTGGGTAAGGATAAAACTGCAGAAGAGAGGAGAATTGCCATCTGCATATTTGATGATATTGCAGAGCATTGCCGTGAAGCTGCTCGTAAGTATTATGATACTTATGTTCCTTTCCTACTGGAAGCTTGCAATGATGAAAGTTCAGATGTTCGTCAGGCTGCTGTTTATGGTGTCGGTCTTTGTGCGGAGTATGGTGGATCTGTGTTCAAACCTCTTGTTGGTGAAGCTCTTTCTAGGTTGGATATGGTGATCAGGAACCCCAGTGCACAACACTCTGACAACATGATGGCATATGATAATGCTGTTTCAGCTCTTGGGAAAATATGCCAGTTCCACCGTGATAGCATAGATGCACCACAGCTTGTCCCTGCCTGGTTAGGTTGTTTACCTATAAAAGGTGATTTGATTGAGGCCAAGGTTGTCCATGACCAGCTGTGTTCAATGGTTGAAAGATCGGATAGAGAGCTTTTGGGTCCCAATAATCAATATCTTCCGAAAATAGTTGCAGTTTTTGCTGAGGTTATATGTGCGGGTAAAGACTTGGCAACTGAGCAAACAACTAGTCGAATGATTAATTTATTAAGGCAGCTTCAACAAACCTTACCGCCCTCTACCCTTGCATCAACCTGGTCATCCTTGCAACCGCAGCAACAGCTCGCATTACAATTTATGCTCTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

1113

Amino Acids

123.34

Weight (kDa)

4.86

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_IPO5 PF25780 116 - 271 3.4e-35 IPO5-like, TPR repeats
Importin_rep_4 PF18808 282 - 373 1e-21 Importin repeat
HEAT_EZ PF13513 389 - 442 1.3e-06 HEAT-like repeat
TOR1L1_N PF24714 390 - 575 1.4e-09 TORTIFOLIA1 N-terminal
HEAT_GCN1 PF23271 397 - 552 4.6e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EZ PF13513 472 - 526 3.7e-07 HEAT-like repeat
TPR_IMB1 PF25574 495 - 644 1.3e-11 Importin subunit beta-1-like, TPR repeats
Importin_rep_6 PF18829 781 - 892 1.2e-17 Importin repeat 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19820
fragaria_vesca FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 594
Acc36I ACCTGC 1 cut(s) 1750
Acc65I GGTACC 1 cut(s) 1483
AccB1I GGYRCC 2 cut(s) 573, 1483
AccBSI CCGCTC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 104
AccII CGCG 2 cut(s) 206, 734
AclWI GGATC 6 cut(s) 5, 232, 1271, 1798, 1805, 2860
AcoI YGGCCR 6 cut(s) 3, 42, 419, 765, 870, 894
AcsI RAATTY 4 cut(s) 1264, 1345, 2153, 2337
AcuI CTGAAG 5 cut(s) 200, 809, 1422, 1895, 2546
AcvI CACGTG 1 cut(s) 1289
AcyI GRCGYC 2 cut(s) 168, 591
AfaI GTAC 5 cut(s) 1422, 1446, 1485, 1490, 1521
AfeI AGCGCT 1 cut(s) 2301
AflII CTTAAG 1 cut(s) 1925
AflIII ACRYGT 2 cut(s) 660, 1288
AgeI ACCGGT 1 cut(s) 570
AhlI ACTAGT 3 cut(s) 1046, 2033, 3215
AjuI GAANNNNNNNTTGG 2 cut(s) 1631, 1663
AleI CACNNNNGTG 1 cut(s) 1287
Alw21I GWGCWC 4 cut(s) 51, 390, 2208, 2923
Alw26I GTCTC 4 cut(s) 385, 457, 1037, 2250
Alw44I GTGCAC 1 cut(s) 2919
AlwI GGATC 6 cut(s) 5, 232, 1271, 1798, 1805, 2860
AlwNI CAGNNNCTG 3 cut(s) 40, 1022, 1583
Ama87I CYCGRG 2 cut(s) 746, 917
Aor51HI AGCGCT 1 cut(s) 2301
ApaI GGGCCC 1 cut(s) 277
ApaLI GTGCAC 1 cut(s) 2919
ApoI RAATTY 4 cut(s) 1264, 1345, 2153, 2337
ArsI GACNNNNNNTTYG 4 cut(s) 850, 882, 3202, 3234
AseI ATTAAT 2 cut(s) 1308, 3228
AsiGI ACCGGT 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 1268
Asp718I GGTACC 1 cut(s) 1483
AspLEI GCGC 4 cut(s) 208, 736, 916, 2302
AsuC2I CCSGG 5 cut(s) 65, 781, 862, 918, 919
AsuII TTCGAA 1 cut(s) 75
AvaI CYCGRG 2 cut(s) 746, 917
AvaII GGWCC 9 cut(s) 61, 120, 187, 424, 670, 858, 985, 1546, 3128
AxyI CCTNAGG 2 cut(s) 788, 2328
BaeGI GKGCMC 2 cut(s) 277, 2923
BaeI ACNNNNGTAYC 2 cut(s) 2745, 2778
BalI TGGCCA 1 cut(s) 872
BanI GGYRCC 2 cut(s) 573, 1483
BanII GRGCYC 4 cut(s) 277, 390, 780, 2208
BauI CACGAG 1 cut(s) 1416
BbrPI CACGTG 1 cut(s) 1289
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 4 cut(s) 51, 390, 2208, 2923
BbvCI CCTCAGC 1 cut(s) 3171
BccI CCATC 5 cut(s) 1490, 1537, 1955, 2702, 2935
BceAI ACGGC 5 cut(s) 29, 1072, 1163, 2073, 2712
BclI TGATCA 2 cut(s) 2119, 2904
BcnI CCSGG 5 cut(s) 65, 781, 862, 918, 919
BcoDI GTCTC 4 cut(s) 385, 457, 1037, 2250
BcuI ACTAGT 3 cut(s) 1046, 2033, 3215
BfaI CTAG 7 cut(s) 1047, 1845, 2034, 2460, 2564, 2889, 3216
BfmI CTRYAG 4 cut(s) 1014, 1526, 1950, 2673
BfoI RGCGCY 2 cut(s) 917, 2303
BfrI CTTAAG 1 cut(s) 1925
BfuAI ACCTGC 1 cut(s) 1750
BmcAI AGTACT 1 cut(s) 1446
Bme18I GGWCC 9 cut(s) 61, 120, 187, 424, 670, 858, 985, 1546, 3128
BmeT110I CYCGRG 2 cut(s) 746, 917
BmrI ACTGGG 1 cut(s) 2910
BmuI ACTGGG 1 cut(s) 2910
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 6 cut(s) 329, 361, 827, 859, 1199, 1231
BpmI CTGGAG 5 cut(s) 182, 458, 869, 1410, 2063
Bpu10I CCTNAGC 1 cut(s) 3171
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 290, 2232
BpuMI CCSGG 5 cut(s) 65, 781, 862, 918, 919
BsaAI YACGTR 2 cut(s) 1289, 1685
BsaBI GATNNNNATC 1 cut(s) 2024
BsaHI GRCGYC 2 cut(s) 168, 591
BsaWI WCCGGW 2 cut(s) 570, 716
BsaXI ACNNNNNCTCC 6 cut(s) 365, 395, 437, 467, 506, 536
Bse118I RCCGGY 2 cut(s) 570, 767
Bse1I ACTGG 8 cut(s) 415, 631, 768, 1010, 2242, 2778, 2916, 2983
Bse21I CCTNAGG 2 cut(s) 788, 2328
Bse3DI GCAATG 6 cut(s) 1185, 1781, 2202, 2312, 2722, 2791
Bse8I GATNNNNATC 1 cut(s) 2024
BseJI GATNNNNATC 1 cut(s) 2024
BseMI GCAATG 6 cut(s) 1185, 1781, 2202, 2312, 2722, 2791
BseNI ACTGG 8 cut(s) 415, 631, 768, 1010, 2242, 2778, 2916, 2983
BseRI GAGGAG 8 cut(s) 233, 289, 308, 313, 445, 571, 805, 2698
BseSI GKGCMC 2 cut(s) 277, 2923
BseYI CCCAGC 1 cut(s) 540
BsgI GTGCAG 3 cut(s) 1250, 1450, 2396
Bsh1236I CGCG 2 cut(s) 206, 734
BshNI GGYRCC 2 cut(s) 573, 1483
BshTI ACCGGT 1 cut(s) 570
BsiHKAI GWGCWC 4 cut(s) 51, 390, 2208, 2923
BsiHKCI CYCGRG 2 cut(s) 746, 917
BsiSI CCGG 9 cut(s) 65, 422, 571, 717, 768, 781, 862, 918, 2201
BslFI GGGAC 5 cut(s) 250, 671, 871, 3005, 3114
BsmAI GTCTC 4 cut(s) 385, 457, 1037, 2250
BsmBI CGTCTC 2 cut(s) 385, 457
BsmFI GGGAC 5 cut(s) 250, 671, 871, 3005, 3114
BsoBI CYCGRG 2 cut(s) 746, 917
Bsp119I TTCGAA 1 cut(s) 75
Bsp120I GGGCCC 1 cut(s) 273
Bsp1286I GDGCHC 6 cut(s) 51, 277, 390, 780, 2208, 2923
BspFNI CGCG 2 cut(s) 206, 734
BspHI TCATGA 1 cut(s) 969
BspMAI CTGCAG 3 cut(s) 1018, 1954, 2677
BspMI ACCTGC 1 cut(s) 1750
BspPI GGATC 6 cut(s) 5, 232, 1271, 1798, 1805, 2860
BspQI GCTCTTC 1 cut(s) 2544
BspT104I TTCGAA 1 cut(s) 75
BspT107I GGYRCC 2 cut(s) 573, 1483
BspTI CTTAAG 1 cut(s) 1925
BsrBI CCGCTC 1 cut(s) 314
BsrDI GCAATG 6 cut(s) 1185, 1781, 2202, 2312, 2722, 2791
BsrFI RCCGGY 2 cut(s) 570, 767
BsrI ACTGG 8 cut(s) 415, 631, 768, 1010, 2242, 2778, 2916, 2983
BssAI RCCGGY 2 cut(s) 570, 767
BssNI GRCGYC 2 cut(s) 168, 591
BssSI CACGAG 1 cut(s) 1416
BssT1I CCWWGG 4 cut(s) 1861, 2111, 2487, 3069
Bst2BI CACGAG 1 cut(s) 1416
Bst4CI ACNGT 5 cut(s) 379, 1258, 1488, 2005, 2993
Bst6I CTCTTC 5 cut(s) 405, 494, 2151, 2544, 2673
BstACI GRCGYC 2 cut(s) 168, 591
BstAFI CTTAAG 1 cut(s) 1925
BstAPI GCANNNNNTGC 3 cut(s) 1310, 1580, 3167
BstBAI YACGTR 2 cut(s) 1289, 1685
BstBI TTCGAA 1 cut(s) 75
BstENI CCTNNNNNAGG 5 cut(s) 56, 652, 1241, 1865, 3048
BstFNI CGCG 2 cut(s) 206, 734
BstH2I RGCGCY 2 cut(s) 917, 2303
BstHHI GCGC 4 cut(s) 208, 736, 916, 2302
BstMAI GTCTC 4 cut(s) 385, 457, 1037, 2250
BstNSI RCATGY 2 cut(s) 664, 2071
BstSFI CTRYAG 4 cut(s) 1014, 1526, 1950, 2673
BstSLI GKGCMC 2 cut(s) 277, 2923
BstUI CGCG 2 cut(s) 206, 734
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 3 cut(s) 1276, 1790, 2852
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 3 cut(s) 1276, 1790, 2852
Bsu36I CCTNAGG 2 cut(s) 788, 2328
BtgZI GCGATG 1 cut(s) 502
BtsI GCAGTG 2 cut(s) 445, 1011
BtsIMutI CAGTG 8 cut(s) 422, 445, 638, 771, 1011, 1101, 1462, 2923
BveI ACCTGC 1 cut(s) 1750
CaiI CAGNNNCTG 3 cut(s) 40, 1022, 1583
CciI TCATGA 1 cut(s) 969
CfoI GCGC 4 cut(s) 208, 736, 916, 2302
Cfr10I RCCGGY 2 cut(s) 570, 767
Cfr9I CCCGGG 1 cut(s) 917
CpoI CGGWCCG 1 cut(s) 120
CseI GACGC 2 cut(s) 176, 439
CsiI ACCWGGT 1 cut(s) 3281
Csp6I GTAC 5 cut(s) 1421, 1445, 1484, 1489, 1520
CspAI ACCGGT 1 cut(s) 570
CspCI CAANNNNNGTGG 2 cut(s) 1077, 1112
CspI CGGWCCG 1 cut(s) 120
CviQI GTAC 5 cut(s) 1421, 1445, 1484, 1489, 1520
EaeI YGGCCR 6 cut(s) 3, 42, 419, 765, 870, 894
Eam1104I CTCTTC 5 cut(s) 405, 494, 2151, 2544, 2673
EarI CTCTTC 5 cut(s) 405, 494, 2151, 2544, 2673
EciI GGCGGA 2 cut(s) 236, 574
Ecl136II GAGCTC 2 cut(s) 388, 2206
Eco130I CCWWGG 4 cut(s) 1861, 2111, 2487, 3069
Eco147I AGGCCT 1 cut(s) 707
Eco24I GRGCYC 4 cut(s) 277, 390, 780, 2208
Eco47I GGWCC 9 cut(s) 61, 120, 187, 424, 670, 858, 985, 1546, 3128
Eco47III AGCGCT 1 cut(s) 2301
Eco53kI GAGCTC 2 cut(s) 388, 2206
Eco57I CTGAAG 5 cut(s) 200, 809, 1422, 1895, 2546
Eco72I CACGTG 1 cut(s) 1289
Eco81I CCTNAGG 2 cut(s) 788, 2328
Eco88I CYCGRG 2 cut(s) 746, 917
EcoICRI GAGCTC 2 cut(s) 388, 2206
EcoNI CCTNNNNNAGG 5 cut(s) 56, 652, 1241, 1865, 3048
EcoO109I RGGNCCY 5 cut(s) 61, 670, 742, 858, 3128
EcoRI GAATTC 1 cut(s) 1264
EcoT14I CCWWGG 4 cut(s) 1861, 2111, 2487, 3069
EcoT22I ATGCAT 2 cut(s) 2362, 2420
EcoT38I GRGCYC 4 cut(s) 277, 390, 780, 2208
ErhI CCWWGG 4 cut(s) 1861, 2111, 2487, 3069
Esp3I CGTCTC 2 cut(s) 385, 457
FalI AAGNNNNNCTT 4 cut(s) 2505, 2537, 2538, 2570
FaqI GGGAC 5 cut(s) 250, 671, 871, 3005, 3114
FauI CCCGC 3 cut(s) 230, 284, 3178
FauNDI CATATG 1 cut(s) 2947
FbaI TGATCA 2 cut(s) 2119, 2904
FblI GTMKAC 1 cut(s) 104
FriOI GRGCYC 4 cut(s) 277, 390, 780, 2208
FspBI CTAG 7 cut(s) 1047, 1845, 2034, 2460, 2564, 2889, 3216
GlaI GCGC 4 cut(s) 207, 735, 915, 2301
GsaI CCCAGC 1 cut(s) 544
GsuI CTGGAG 5 cut(s) 182, 458, 869, 1410, 2063
HaeII RGCGCY 2 cut(s) 917, 2303
HapII CCGG 9 cut(s) 65, 422, 571, 717, 768, 781, 862, 918, 2201
HgaI GACGC 2 cut(s) 176, 439
HhaI GCGC 4 cut(s) 208, 736, 916, 2302
Hin1I GRCGYC 2 cut(s) 168, 591
Hin6I GCGC 4 cut(s) 206, 734, 914, 2300
HinP1I GCGC 4 cut(s) 206, 734, 914, 2300
HincII GTYRAC 3 cut(s) 105, 1254, 1329
HindII GTYRAC 3 cut(s) 105, 1254, 1329
HindIII AAGCTT 3 cut(s) 1511, 2630, 2778
HinfI GANTC 9 cut(s) 218, 260, 338, 479, 597, 1586, 1955, 2025, 2261
HpaII CCGG 9 cut(s) 65, 422, 571, 717, 768, 781, 862, 918, 2201
Hpy166II GTNNAC 7 cut(s) 105, 342, 1183, 1254, 1329, 2921, 3041
Hpy188III TCNNGA 9 cut(s) 722, 787, 970, 1606, 1729, 1774, 2042, 2460, 2908
Hpy8I GTNNAC 7 cut(s) 105, 342, 1183, 1254, 1329, 2921, 3041
Hpy99I CGWCG 4 cut(s) 106, 109, 593, 998
HpyAV CCTTC 8 cut(s) 848, 1726, 1911, 2089, 2093, 2230, 2507, 2521
HpyCH4III ACNGT 5 cut(s) 379, 1258, 1488, 2005, 2993
HpyCH4IV ACGT 4 cut(s) 591, 1288, 1684, 2212
HpySE526I ACGT 4 cut(s) 591, 1288, 1684, 2212
Hsp92I GRCGYC 2 cut(s) 168, 591
HspAI GCGC 4 cut(s) 206, 734, 914, 2300
KflI GGGWCCC 2 cut(s) 858, 3128
KpnI GGTACC 1 cut(s) 1487
KroI GCCGGC 1 cut(s) 767
KroNI GCCGGC 1 cut(s) 769
Ksp22I TGATCA 2 cut(s) 2119, 2904
LguI GCTCTTC 1 cut(s) 2544
MabI ACCWGGT 1 cut(s) 3281
MaeI CTAG 7 cut(s) 1047, 1845, 2034, 2460, 2564, 2889, 3216
MaeII ACGT 4 cut(s) 591, 1288, 1684, 2212
MaeIII GTNAC 5 cut(s) 1049, 1590, 1830, 1936, 2063
MbiI CCGCTC 1 cut(s) 314
MfeI CAATTG 1 cut(s) 1322
MflI RGATCY 3 cut(s) 1276, 1790, 2852
MhlI GDGCHC 6 cut(s) 51, 277, 390, 780, 2208, 2923
MlsI TGGCCA 1 cut(s) 872
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 5 cut(s) 212, 254, 347, 488, 2270
MmeI TCCRAC 6 cut(s) 38, 483, 963, 1696, 2156, 2874
Mox20I TGGCCA 1 cut(s) 872
Mph1103I ATGCAT 2 cut(s) 2362, 2420
MroNI GCCGGC 1 cut(s) 767
MroXI GAANNNNTTC 1 cut(s) 1268
MscI TGGCCA 1 cut(s) 872
MseI TTAA 6 cut(s) 1308, 1562, 1653, 1926, 3228, 3236
MslI CAYNNNNRTG 6 cut(s) 233, 968, 974, 1287, 1994, 2727
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 5 cut(s) 40, 118, 799, 2186, 3088
MspCI CTTAAG 1 cut(s) 1925
MspI CCGG 9 cut(s) 65, 422, 571, 717, 768, 781, 862, 918, 2201
MunI CAATTG 1 cut(s) 1322
MvnI CGCG 2 cut(s) 206, 734
NaeI GCCGGC 1 cut(s) 769
NciI CCSGG 5 cut(s) 65, 781, 862, 918, 919
NdeI CATATG 1 cut(s) 2947
NgoMIV GCCGGC 1 cut(s) 767
NmeAIII GCCGAG 4 cut(s) 154, 859, 922, 1231
NmuCI GTSAC 2 cut(s) 1590, 1936
NsiI ATGCAT 2 cut(s) 2362, 2420
NspI RCATGY 2 cut(s) 664, 2071
NspV TTCGAA 1 cut(s) 75
OliI CACNNNNGTG 1 cut(s) 1287
PaeR7I CTCGAG 1 cut(s) 746
PagI TCATGA 1 cut(s) 969
PceI AGGCCT 1 cut(s) 707
PciI ACATGT 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 2544
PcsI WCGNNNNNNNCGW 2 cut(s) 999, 1681
PdiI GCCGGC 1 cut(s) 769
PdmI GAANNNNTTC 1 cut(s) 1268
PfeI GAWTC 4 cut(s) 597, 1586, 1955, 2025
PflFI GACNNNGTC 1 cut(s) 377
PfoI TCCNGGA 3 cut(s) 474, 666, 1548
PinAI ACCGGT 1 cut(s) 570
PleI GAGTC 5 cut(s) 212, 254, 346, 487, 2269
PmaCI CACGTG 1 cut(s) 1289
PmlI CACGTG 1 cut(s) 1289
PpsI GAGTC 5 cut(s) 212, 254, 346, 487, 2269
Ppu21I YACGTR 2 cut(s) 1289, 1685
PpuMI RGGWCCY 4 cut(s) 61, 670, 858, 3128
PscI ACATGT 1 cut(s) 660
PshBI ATTAAT 2 cut(s) 1308, 3228
Psp124BI GAGCTC 2 cut(s) 390, 2208
Psp5II RGGWCCY 4 cut(s) 61, 670, 858, 3128
PspCI CACGTG 1 cut(s) 1289
PspFI CCCAGC 1 cut(s) 540
PspOMI GGGCCC 1 cut(s) 273
PspPPI RGGWCCY 4 cut(s) 61, 670, 858, 3128
PspXI VCTCGAGB 1 cut(s) 746
PstI CTGCAG 3 cut(s) 1018, 1954, 2677
PstNI CAGNNNCTG 3 cut(s) 40, 1022, 1583
PsuI RGATCY 3 cut(s) 1276, 1790, 2852
PsyI GACNNNGTC 1 cut(s) 377
PvuII CAGCTG 4 cut(s) 40, 799, 2186, 3088
RsaI GTAC 5 cut(s) 1422, 1446, 1485, 1490, 1521
RsaNI GTAC 5 cut(s) 1421, 1445, 1484, 1489, 1520
RseI CAYNNNNRTG 6 cut(s) 233, 968, 974, 1287, 1994, 2727
Rsr2I CGGWCCG 1 cut(s) 120
RsrII CGGWCCG 1 cut(s) 120
SacI GAGCTC 2 cut(s) 390, 2208
SalI GTCGAC 1 cut(s) 103
SapI GCTCTTC 1 cut(s) 2544
SaqAI TTAA 6 cut(s) 1308, 1562, 1653, 1926, 3228, 3236
ScaI AGTACT 1 cut(s) 1446
SchI GAGTC 5 cut(s) 212, 254, 347, 488, 2270
SduI GDGCHC 6 cut(s) 51, 277, 390, 780, 2208, 2923
SexAI ACCWGGT 1 cut(s) 3281
SfcI CTRYAG 4 cut(s) 1014, 1526, 1950, 2673
Sfr274I CTCGAG 1 cut(s) 746
SfuI TTCGAA 1 cut(s) 75
SgrDI CGTCGACG 1 cut(s) 103
SinI GGWCC 9 cut(s) 61, 120, 187, 424, 670, 858, 985, 1546, 3128
SlaI CTCGAG 1 cut(s) 746
SmaI CCCGGG 1 cut(s) 919
SmiMI CAYNNNNRTG 6 cut(s) 233, 968, 974, 1287, 1994, 2727
SmlI CTYRAG 4 cut(s) 305, 746, 1925, 2247
SmoI CTYRAG 4 cut(s) 305, 746, 1925, 2247
SpeI ACTAGT 3 cut(s) 1046, 2033, 3215
SseBI AGGCCT 1 cut(s) 707
SspMI CTAG 7 cut(s) 1047, 1845, 2034, 2460, 2564, 2889, 3216
SstI GAGCTC 2 cut(s) 390, 2208
StuI AGGCCT 1 cut(s) 707
StyI CCWWGG 4 cut(s) 1861, 2111, 2487, 3069
TaaI ACNGT 5 cut(s) 379, 1258, 1488, 2005, 2993
TaiI ACGT 4 cut(s) 594, 1291, 1687, 2215
TaqI TCGA 7 cut(s) 75, 104, 258, 747, 759, 1239, 3220
TaqII GACCGA 1 cut(s) 2819
TatI WGTACW 3 cut(s) 1420, 1444, 1519
TauI GCSGC 5 cut(s) 8, 206, 252, 605, 1436
TfiI GAWTC 4 cut(s) 597, 1586, 1955, 2025
Tru1I TTAA 6 cut(s) 1308, 1562, 1653, 1926, 3228, 3236
Tru9I TTAA 6 cut(s) 1308, 1562, 1653, 1926, 3228, 3236
TscAI CASTG 8 cut(s) 422, 452, 638, 778, 1018, 1101, 1462, 2923
TseFI GTSAC 2 cut(s) 1590, 1936
Tsp45I GTSAC 2 cut(s) 1590, 1936
TspGWI ACGGA 4 cut(s) 223, 550, 1128, 1346
TspMI CCCGGG 1 cut(s) 917
TspRI CASTG 8 cut(s) 422, 452, 638, 778, 1018, 1101, 1462, 2923
Tth111I GACNNNGTC 1 cut(s) 377
Vha464I CTTAAG 1 cut(s) 1925
VneI GTGCAC 1 cut(s) 2919
VpaK11BI GGWCC 9 cut(s) 61, 120, 187, 424, 670, 858, 985, 1546, 3128
VspI ATTAAT 2 cut(s) 1308, 3228
XagI CCTNNNNNAGG 5 cut(s) 56, 652, 1241, 1865, 3048
XapI RAATTY 4 cut(s) 1264, 1345, 2153, 2337
XbaI TCTAGA 1 cut(s) 2459
XceI RCATGY 2 cut(s) 664, 2071
XcmI CCANNNNNNNNNTGG 1 cut(s) 1293
XhoI CTCGAG 1 cut(s) 746
XmaI CCCGGG 1 cut(s) 917
XmiI GTMKAC 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 1268
XspI CTAG 7 cut(s) 1047, 1845, 2034, 2460, 2564, 2889, 3216
ZraI GACGTC 1 cut(s) 592
ZrmI AGTACT 1 cut(s) 1446
Zsp2I ATGCAT 2 cut(s) 2362, 2420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.