Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000344

Physical Location & Seq

Genomic Coordinates Forward (+)

368382 .. 375997

Gene Structure 7616 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1004700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3348 bp

ATGGCCGAGTCGACTCAGCTACAGCAAGCTCAACTGGCGACGATTCTCGGTCCCGACCCGGCTCCTTTCCAAACCCTAATCTCCCACCTCATGTCCTCCGCCAATGAGCAGCGCTCCCAGGCCGAGCTCCTCTTCAATCTCTGCAAGCAGACCGATCCTGACTCCCTCTGCCTCAAACTCGCTCACCTCCTCCAGTTCTCTCCCGCCGCCGAGGCCCGAGCAATGTCTGCCATTCTCCTCCGCAAGCAGCTCACTCGCGACGACTCGTACCTCTGGCCCCGATTGAATCCGACGACCCAATCCTCGCTGAAAACGATTCTCCTGACCTGTATTCAGCAGGCGGACAACAAATCTATCTCCAAGAAGCTCTGCGATACAATTTCCGAGCTCGCCTCCGGAATCTTGCCCGATAACGGGTGGCCGGAGCTCTTGCCGTTCATGTTCCAATGCGTCTCGTCCGATTCGCCCAAGCTTCAGGAATCGGCGTTTTTGATATTTGCTCAGTTATCGCAGTACATCGGCGACACCATGGTGCCGCACATAAAGGAGTTGCACGCCGTGTTCTTGCATAGCCTGGGGAACTCGCCGAACCCCGACGTTAAAATCGCAGCTTTGAACGCCGTCATCAATTTCATCCAGTGTTTGACAAGCTCTGCCGAAAGGGATAGGTTTCAGGACCTGTTGCCGGCGATGATGAAGACGTTGATGGAGTCTTTGAACAACGGGAATGAGGCCACAGCACAGGACGCTCTTGAATTGTTGATTGAATTGGCAGGGACCGAGCCCAGGTTCCTCAGGCGGCAGATTGTGGAGGTTGTCGGGGCAATGTTACAGATTGCCGAGGCTGACACCTTGGAAGAAGCTACCCGGCACTTGGCAATTGAGTTCGTAATCACCCTTGCTGAGGCCAGGGAACGTGCTCCAGGGATGATGAGAAAGTTGCCACAGTTTATAAGCCGATTGTTTGCCATTTTGATGAGGATGCTTCTGGATATTGAGGACGAACCCTCATGGCATACAGCCGAGAGTGAAGATGAGGATGCGGGTGAGACTGGTAATTACAGTGTTGGGCAGGAGTGTTTGGATAGGCTTGCCATATCCCTGGGTGGGAATACCATTGTTCCCGTGGCATCCGAGCAGTTGCCTGCTTATTTGGCTGCCCCTGAATGGCAGAAGCACCATGCTGCATTGATTGCTCTCGCTCAAATTGCAGAGGGTTGTGGCAAGGTAATGACAAAAAATTTGGAGCAAGTGGTGGCAATGGTCTTGAACTCATTTCAAGATCCACATCCTCGTGTAAGGTGGGCAGCAATTAATGCAATTGGGCAGCTGTCTACAGATTTGGGTCCAGATCTGCAAGTTCAATATCACCAAAGGGTACTCCCGGCATTGGCTGCAGCTATGGATGATTATCAGAATCCCCGCGTTCAGGCACATGCTGCTTCAGCGTTGCTCAACTTCAGTGAGAATTGTACTCCAGATATTTTGACACCTTATTTGGATGGGATAGTGAGTAAACTGCTTGTACTTTTACAGAATGGGAAACAAATGGTGCAAGAGGGAGCCCTTACTGCTTTGGCATCTGTTGCAGATTCATCTCAGGAGCATTTCCAAAAATACTATGATGCAGTTATACCTTACCTGAAAGCTATCTTGGTGAATGCAACTGATAAGTCTAACCGCATGCTTCGTGCCAAATCCATGGAGTGCATTAGCTTGGTTGGAATGGCTGTTGGAAAGGATAAGTTTAGGGATGATGCGAAGCAGGTCATGGAAGTGCTGATGGCTTTACAAGGATCTGGAATGGAGGCAGATGATCCAACGACAAGTTACATGCTACAGGCATGGGCAAGACTCTGCAAGTGCTTAGGACAAGATTTCCTCCCCTACATGAGTGTTGTAATGCCTCCTTTGCTTCACTCTGCTCAACTTAAGCCAGATGTAACCATTACATCTGCAGATGACAATAGTGATATTGATGATTCTGATGATGAAAGTATTGAAACAATTACTCTTGGGGATAAGAGGATTGGAATCAAGACTAGTGTCCTGGAGGAAAAAGCTACTGCTTGCAATATGCTCTGTTGCTATGCTGACGAGTTGAAAGAAGGATTCTTTCCTTGGATTGATCAGGTTGCCCCAACTTTGGTTCCACTTCTTAAATTTTATTTTCACGATGAAGTTAGGAAGGCAGCTGTTTCAGCGATGCCGGAGCTGCTGCTTTCTGCAAAATTAGCCATAGAGAAGGGGCAAGCTCAAGGTCGTAATGAAACCTATATAAAGCAATTGTCTGACTACATTGTTCCAGCTCTGGTGGAAGCATTACACAAGGAACCTGATACAGAAATATGTGCAAATATTTTGGATGCACTAAATGAATGTTTACAGATATCTGGACCGCTTTTAGATGAAAACCAGGTCAGATCCATTGTGGAGGAGATAAAGCAGGTGATCACTGCCAGTTCAAGTAGAAAAAGAGAGAGAGCAGAAAGGACCAAAGCAGAAGACTTTGACGATGAGGAAAAAGAGCTAATTAAAGAGGAAAATGAACAAGAGGAAGAAGTTTTTGATCAAGTGGGGGAAATCTTAGGAACTCTGATTAAAACATTTAAAGCCTCTTTCTTACCTTTCTTTGATGAGCTATCATCGTATCTAACACCAATGTGGGCCAAGGATAAAACACCTGAAGAGAGAAGGATCGCAATATGCATCTTTGATGATGTTGCGGAGCAGTGCCGTGAAGCAGCTTTAAAATATTATGACACATTTCTTCCTTTTCTATTGGAGGCCTGCAATGACGATAACCCTGATGTTCGACAGGCAGCTCTATATGGACTTGGTGTCTGTTCAGAGTTTGGCGGAATAGTAATTAAACCTCTTGTTGGCGAGGCTCTTTCAAGGCTAAATGCTGTGATTCAGCATCCTAATGCCCTGCAAGCTGAAAATGTGATGGCATATGATAATGCTGTTTCTGCTTTAGGAAAAATATGTCAATTTCATCGTGACAGTATTGATGCAGCTCAGGTAATTCCTGCATGGTTGAACTGTTTACCAATTAAAGGTGACTTGATTGAAGCCAAAGTTGTTCATGACCAACTTTGTTCTATGGTGGAAAGGTCTGACAGAGAACTTTTGGGTCTCAACAATCAGTGTCTTCCTAAAATTGTTGCCGTGTTTGCTGAGGTTCTATGCGCGGGTAAGGATTTAGCAACAGAACAAACTGTAAGCAGAATGATTAATCTATTAAAGCAGCTTCAGCAGACTTTGCCGCCAGCAACATTGGCCTCAACGTGGTCCTCCTTGCAACCTCAGCAACAGCTTGCACTGCAATCTATCCTCTCCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

1116

Amino Acids

123.37

Weight (kDa)

4.74

Isoelectric Point (pI)

46.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	116 - 273	8.1e-37	IPO5-like, TPR repeats
Importin_rep_4	PF18808	284 - 375	3.2e-22	Importin repeat
HEAT_2	PF13646	385 - 484	7e-08	HEAT repeats
HEAT_EZ	PF13513	390 - 444	2.4e-08	HEAT-like repeat
TOR1L1_N	PF24714	396 - 575	5.8e-09	TORTIFOLIA1 N-terminal
HEAT_GCN1	PF23271	398 - 543	2.9e-07	Stalled ribosome sensor GCN1-like, HEAT repeats
Cnd1	PF12717	412 - 549	4.7e-06	non-SMC mitotic condensation complex subunit 1
HEAT_EZ	PF13513	474 - 528	2.8e-07	HEAT-like repeat
TPR_IMB1	PF25574	476 - 645	8.1e-11	Importin subunit beta-1-like, TPR repeats
Importin_rep_6	PF18829	784 - 887	1.2e-19	Importin repeat 6
HEAT_EZ	PF13513	895 - 947	3.8e-07	HEAT-like repeat
HEAT	PF02985	923 - 951	4.3e-06	HEAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19820
fragaria_vesca	FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica	MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica	Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis	pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis	RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata	RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora	Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii	Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa	Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha	Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana	Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	953
AarI	CACCTGC	1 cut(s)	2437
Acc36I	ACCTGC	2 cut(s)	1756, 2437
AccB1I	GGYRCC	1 cut(s)	532
AccI	GTMKAC	2 cut(s)	11, 1334
AccII	CGCG	3 cut(s)	258, 1425, 3194
AccIII	TCCGGA	1 cut(s)	395
AclWI	GGATC	6 cut(s)	149, 1277, 1804, 1811, 2418, 2705
AcoI	YGGCCR	2 cut(s)	3, 419
AcsI	RAATTY	2 cut(s)	1240, 2162
AcuI	CTGAAG	5 cut(s)	458, 1428, 1444, 2706, 3239
AdeI	CACNNNGTG	1 cut(s)	559
AfaI	GTAC	5 cut(s)	269, 515, 1380, 1474, 1527
AfeI	AGCGCT	1 cut(s)	113
AflII	CTTAAG	1 cut(s)	1931
AhdI	GACNNNNNGTC	1 cut(s)	2840
AhlI	ACTAGT	1 cut(s)	2042
AjnI	CCWGG	8 cut(s)	117, 573, 785, 908, 922, 1101, 2049, 2415
AjuI	GAANNNNNNNTTGG	2 cut(s)	1637, 1669
AleI	CACNNNNGTG	2 cut(s)	530, 2662
Alw21I	GWGCWC	4 cut(s)	129, 390, 429, 922
Alw26I	GTCTC	3 cut(s)	457, 1043, 3143
AlwI	GGATC	6 cut(s)	149, 1277, 1804, 1811, 2418, 2705
AlwNI	CAGNNNCTG	1 cut(s)	679
Ama87I	CYCGRG	1 cut(s)	216
Aor13HI	TCCGGA	1 cut(s)	395
Aor51HI	AGCGCT	1 cut(s)	113
ApoI	RAATTY	2 cut(s)	1240, 2162
ArsI	GACNNNNNNTTYG	4 cut(s)	1225, 1257, 1480, 1512
AseI	ATTAAT	2 cut(s)	1314, 3237
AspLEI	GCGC	2 cut(s)	114, 3194
AsuC2I	CCSGG	3 cut(s)	59, 868, 1385
AsuHPI	GGTGA	7 cut(s)	176, 886, 1058, 1361, 1669, 2461, 3074
AvaI	CYCGRG	1 cut(s)	216
AvaII	GGWCC	7 cut(s)	50, 676, 777, 1346, 2396, 2493, 3294
AxyI	CCTNAGG	1 cut(s)	794
BanI	GGYRCC	1 cut(s)	532
BanII	GRGCYC	5 cut(s)	129, 390, 429, 786, 1567
BauI	CACGAG	1 cut(s)	1293
BbsI	GAAGAC	3 cut(s)	704, 2511, 3146
Bbv12I	GWGCWC	4 cut(s)	129, 390, 429, 922
BbvCI	CCTCAGC	3 cut(s)	903, 3180, 3309
BccI	CCATC	4 cut(s)	700, 1496, 1777, 2944
BceAI	ACGGC	5 cut(s)	418, 542, 605, 2721, 3155
BcgI	CGANNNNNNTGC	2 cut(s)	236, 270
BciT130I	CCWGG	8 cut(s)	119, 575, 787, 910, 924, 1103, 2051, 2417
BclI	TGATCA	3 cut(s)	2128, 2451, 2569
BcnI	CCSGG	3 cut(s)	59, 868, 1385
BcoDI	GTCTC	3 cut(s)	457, 1043, 3143
BcuI	ACTAGT	1 cut(s)	2042
BfaI	CTAG	1 cut(s)	2043
BfmI	CTRYAG	5 cut(s)	20, 1335, 1395, 1838, 1956
BfoI	RGCGCY	1 cut(s)	115
BfrI	CTTAAG	1 cut(s)	1931
BfuAI	ACCTGC	2 cut(s)	1756, 2437
BglI	GCCNNNNNGGC	1 cut(s)	212
BglII	AGATCT	1 cut(s)	1351
Bme18I	GGWCC	7 cut(s)	50, 676, 777, 1346, 2396, 2493, 3294
BmeRI	GACNNNNNGTC	1 cut(s)	2840
BmeT110I	CYCGRG	1 cut(s)	216
BoxI	GACNNNNGTC	1 cut(s)	2045
BpiI	GAAGAC	3 cut(s)	704, 2511, 3146
BplI	GAGNNNNNCTC	4 cut(s)	98, 130, 377, 409
BpmI	CTGGAG	4 cut(s)	176, 906, 1461, 2072
Bpu10I	CCTNAGC	5 cut(s)	903, 1867, 3021, 3180, 3309
BpuEI	CTTGAG	1 cut(s)	2241
BpuMI	CCSGG	3 cut(s)	59, 868, 1385
BsaBI	GATNNNNATC	3 cut(s)	1287, 1410, 2033
BsaI	GGTCTC	1 cut(s)	3143
BsaWI	WCCGGW	1 cut(s)	395
Bse118I	RCCGGY	1 cut(s)	685
Bse1I	ACTGG	5 cut(s)	39, 193, 637, 1057, 2460
Bse21I	CCTNAGG	1 cut(s)	794
Bse3DI	GCAATG	4 cut(s)	228, 831, 1266, 2800
Bse8I	GATNNNNATC	3 cut(s)	1287, 1410, 2033
BseAI	TCCGGA	1 cut(s)	395
BseBI	CCWGG	8 cut(s)	119, 575, 787, 910, 924, 1103, 2051, 2417
BseJI	GATNNNNATC	3 cut(s)	1287, 1410, 2033
BseMI	GCAATG	4 cut(s)	228, 831, 1266, 2800
BseMII	CTCAG	8 cut(s)	29, 515, 808, 894, 1613, 3035, 3171, 3323
BseNI	ACTGG	5 cut(s)	39, 193, 637, 1057, 2460
BseRI	GAGGAG	5 cut(s)	119, 179, 227, 2450, 3331
Bsh1236I	CGCG	3 cut(s)	258, 1425, 3194
BshNI	GGYRCC	1 cut(s)	532
BsiHKAI	GWGCWC	4 cut(s)	129, 390, 429, 922
BsiHKCI	CYCGRG	1 cut(s)	216
BsiSI	CCGG	7 cut(s)	59, 396, 422, 686, 868, 1385, 2210
BslFI	GGGAC	2 cut(s)	36, 790
BsmAI	GTCTC	3 cut(s)	457, 1043, 3143
BsmBI	CGTCTC	1 cut(s)	457
BsmFI	GGGAC	2 cut(s)	36, 790
BsmI	GAATGC	1 cut(s)	1666
Bso31I	GGTCTC	1 cut(s)	3143
BsoBI	CYCGRG	1 cut(s)	216
Bsp1286I	GDGCHC	6 cut(s)	129, 390, 429, 786, 922, 1567
Bsp13I	TCCGGA	1 cut(s)	395
Bsp19I	CCATGG	2 cut(s)	528, 1701
Bsp68I	TCGCGA	1 cut(s)	258
BspCNI	CTCAG	8 cut(s)	28, 514, 807, 895, 1612, 3034, 3172, 3322
BspEI	TCCGGA	1 cut(s)	395
BspFNI	CGCG	3 cut(s)	258, 1425, 3194
BspHI	TCATGA	1 cut(s)	3088
BspMAI	CTGCAG	2 cut(s)	1399, 1960
BspMI	ACCTGC	2 cut(s)	1756, 2437
BspPI	GGATC	6 cut(s)	149, 1277, 1804, 1811, 2418, 2705
BspT107I	GGYRCC	1 cut(s)	532
BspTI	CTTAAG	1 cut(s)	1931
BspTNI	GGTCTC	1 cut(s)	3143
BsrDI	GCAATG	4 cut(s)	228, 831, 1266, 2800
BsrFI	RCCGGY	1 cut(s)	685
BsrI	ACTGG	5 cut(s)	39, 193, 637, 1057, 2460
BssAI	RCCGGY	1 cut(s)	685
BssSI	CACGAG	1 cut(s)	1293
BssT1I	CCWWGG	5 cut(s)	528, 852, 1701, 2120, 2670
Bst2BI	CACGAG	1 cut(s)	1293
Bst2UI	CCWGG	8 cut(s)	119, 575, 787, 910, 924, 1103, 2051, 2417
Bst4CI	ACNGT	5 cut(s)	948, 1064, 3008, 3047, 3223
Bst6I	CTCTTC	2 cut(s)	137, 2682
BstAFI	CTTAAG	1 cut(s)	1931
BstAPI	GCANNNNNTGC	7 cut(s)	227, 1193, 1316, 1394, 1439, 1586, 3265
BstDSI	CCRYGG	3 cut(s)	528, 1125, 1701
BstENI	CCTNNNNNAGG	1 cut(s)	902
BstFNI	CGCG	3 cut(s)	258, 1425, 3194
BstH2I	RGCGCY	1 cut(s)	115
BstHHI	GCGC	2 cut(s)	114, 3194
BstMAI	GTCTC	3 cut(s)	457, 1043, 3143
BstNI	CCWGG	8 cut(s)	119, 575, 787, 910, 924, 1103, 2051, 2417
BstNSI	RCATGY	3 cut(s)	1439, 1687, 1837
BstPAI	GACNNNNGTC	1 cut(s)	2045
BstSFI	CTRYAG	5 cut(s)	20, 1335, 1395, 1838, 1956
BstUI	CGCG	3 cut(s)	258, 1425, 3194
BstV2I	GAAGAC	3 cut(s)	704, 2511, 3146
BstX2I	RGATCY	4 cut(s)	1282, 1351, 1796, 2423
BstXI	CCANNNNNNTGG	2 cut(s)	1102, 1702
BstYI	RGATCY	4 cut(s)	1282, 1351, 1796, 2423
Bsu36I	CCTNAGG	1 cut(s)	794
BtgI	CCRYGG	3 cut(s)	528, 1125, 1701
BtgZI	GCGATG	2 cut(s)	704, 2219
BtsI	GCAGTG	3 cut(s)	2454, 2738, 3323
BtsIMutI	CAGTG	7 cut(s)	644, 1069, 1468, 2454, 2738, 3155, 3323
BtuMI	TCGCGA	1 cut(s)	258
BveI	ACCTGC	2 cut(s)	1756, 2437
CaiI	CAGNNNCTG	1 cut(s)	679
CciI	TCATGA	1 cut(s)	3088
CfoI	GCGC	2 cut(s)	114, 3194
Cfr10I	RCCGGY	1 cut(s)	685
CseI	GACGC	2 cut(s)	439, 755
CsiI	ACCWGGT	1 cut(s)	2415
Csp6I	GTAC	5 cut(s)	268, 514, 1379, 1473, 1526
CviQI	GTAC	5 cut(s)	268, 514, 1379, 1473, 1526
DraI	TTTAAA	2 cut(s)	2611, 2751
DraIII	CACNNNGTG	1 cut(s)	559
DriI	GACNNNNNGTC	1 cut(s)	2840
EaeI	YGGCCR	2 cut(s)	3, 419
Eam1104I	CTCTTC	2 cut(s)	137, 2682
Eam1105I	GACNNNNNGTC	1 cut(s)	2840
EarI	CTCTTC	2 cut(s)	137, 2682
EciI	GGCGGA	3 cut(s)	88, 356, 2874
Ecl136II	GAGCTC	3 cut(s)	127, 388, 427
Eco130I	CCWWGG	5 cut(s)	528, 852, 1701, 2120, 2670
Eco147I	AGGCCT	1 cut(s)	2789
Eco24I	GRGCYC	5 cut(s)	129, 390, 429, 786, 1567
Eco31I	GGTCTC	1 cut(s)	3143
Eco32I	GATATC	1 cut(s)	2391
Eco47I	GGWCC	7 cut(s)	50, 676, 777, 1346, 2396, 2493, 3294
Eco47III	AGCGCT	1 cut(s)	113
Eco53kI	GAGCTC	3 cut(s)	127, 388, 427
Eco57I	CTGAAG	5 cut(s)	458, 1428, 1444, 2706, 3239
Eco81I	CCTNAGG	1 cut(s)	794
Eco88I	CYCGRG	1 cut(s)	216
EcoICRI	GAGCTC	3 cut(s)	127, 388, 427
EcoNI	CCTNNNNNAGG	1 cut(s)	902
EcoO109I	RGGNCCY	1 cut(s)	676
EcoRII	CCWGG	8 cut(s)	117, 573, 785, 908, 922, 1101, 2049, 2415
EcoRV	GATATC	1 cut(s)	2391
EcoT14I	CCWWGG	5 cut(s)	528, 852, 1701, 2120, 2670
EcoT22I	ATGCAT	1 cut(s)	2711
EcoT38I	GRGCYC	5 cut(s)	129, 390, 429, 786, 1567
ErhI	CCWWGG	5 cut(s)	528, 852, 1701, 2120, 2670
Esp3I	CGTCTC	1 cut(s)	457
FaqI	GGGAC	2 cut(s)	36, 790
FauI	CCCGC	4 cut(s)	211, 1036, 1430, 3187
FauNDI	CATATG	1 cut(s)	2956
FbaI	TGATCA	3 cut(s)	2128, 2451, 2569
FblI	GTMKAC	2 cut(s)	11, 1334
FriOI	GRGCYC	5 cut(s)	129, 390, 429, 786, 1567
FspBI	CTAG	1 cut(s)	2043
GlaI	GCGC	2 cut(s)	113, 3193
GsuI	CTGGAG	4 cut(s)	176, 906, 1461, 2072
HaeII	RGCGCY	1 cut(s)	115
HapII	CCGG	7 cut(s)	59, 396, 422, 686, 868, 1385, 2210
HgaI	GACGC	2 cut(s)	439, 755
HhaI	GCGC	2 cut(s)	114, 3194
Hin6I	GCGC	2 cut(s)	112, 3192
HinP1I	GCGC	2 cut(s)	112, 3192
HincII	GTYRAC	1 cut(s)	12
HindII	GTYRAC	1 cut(s)	12
HindIII	AAGCTT	1 cut(s)	470
HpaII	CCGG	7 cut(s)	59, 396, 422, 686, 868, 1385, 2210
HphI	GGTGA	7 cut(s)	176, 886, 1058, 1361, 1669, 2461, 3074
Hpy166II	GTNNAC	5 cut(s)	12, 1335, 1517, 2384, 3050
Hpy8I	GTNNAC	5 cut(s)	12, 1335, 1517, 2384, 3050
Hpy99I	CGWCG	4 cut(s)	43, 263, 295, 599
HpyAV	CCTTC	4 cut(s)	2102, 2182, 2239, 2688
HpyCH4III	ACNGT	5 cut(s)	948, 1064, 3008, 3047, 3223
HpyCH4IV	ACGT	4 cut(s)	597, 701, 916, 3290
HpySE526I	ACGT	4 cut(s)	597, 701, 916, 3290
HspAI	GCGC	2 cut(s)	112, 3192
Kpn2I	TCCGGA	1 cut(s)	395
KroI	GCCGGC	1 cut(s)	685
KroNI	GCCGGC	1 cut(s)	687
Ksp22I	TGATCA	3 cut(s)	2128, 2451, 2569
MabI	ACCWGGT	1 cut(s)	2415
MaeI	CTAG	1 cut(s)	2043
MaeII	ACGT	4 cut(s)	597, 701, 916, 3290
MaeIII	GTNAC	5 cut(s)	828, 1829, 1942, 3002, 3062
MboII	GAAGA	9 cut(s)	124, 709, 869, 1043, 2516, 2570, 2699, 2762, 3146
MfeI	CAATTG	3 cut(s)	879, 1320, 2285
MflI	RGATCY	4 cut(s)	1282, 1351, 1796, 2423
MhlI	GDGCHC	6 cut(s)	129, 390, 429, 786, 922, 1567
MlyI	GAGTC	6 cut(s)	7, 17, 155, 257, 719, 1848
MmeI	TCCRAC	4 cut(s)	314, 1702, 1714, 1844
Mph1103I	ATGCAT	1 cut(s)	2711
MroI	TCCGGA	1 cut(s)	395
MroNI	GCCGGC	1 cut(s)	685
MslI	CAYNNNNRTG	6 cut(s)	530, 974, 1293, 1775, 2662, 2925
MspA1I	CMGCKG	2 cut(s)	1330, 2195
MspCI	CTTAAG	1 cut(s)	1931
MspI	CCGG	7 cut(s)	59, 396, 422, 686, 868, 1385, 2210
MunI	CAATTG	3 cut(s)	879, 1320, 2285
Mva1269I	GAATGC	1 cut(s)	1666
MvaI	CCWGG	8 cut(s)	119, 575, 787, 910, 924, 1103, 2051, 2417
MvnI	CGCG	3 cut(s)	258, 1425, 3194
NaeI	GCCGGC	1 cut(s)	687
NciI	CCSGG	3 cut(s)	59, 868, 1385
NcoI	CCATGG	2 cut(s)	528, 1701
NdeI	CATATG	1 cut(s)	2956
NgoMIV	GCCGGC	1 cut(s)	685
NmeAIII	GCCGAG	5 cut(s)	31, 148, 235, 865, 1048
NmuCI	GTSAC	2 cut(s)	3002, 3062
NruI	TCGCGA	1 cut(s)	258
NsiI	ATGCAT	1 cut(s)	2711
NspI	RCATGY	3 cut(s)	1439, 1687, 1837
OliI	CACNNNNGTG	2 cut(s)	530, 2662
PaeI	GCATGC	1 cut(s)	1687
PagI	TCATGA	1 cut(s)	3088
PaqCI	CACCTGC	1 cut(s)	2437
PasI	CCCWGGG	1 cut(s)	1102
PceI	AGGCCT	1 cut(s)	2789
PcsI	WCGNNNNNNNCGW	1 cut(s)	311
PctI	GAATGC	1 cut(s)	1666
PdiI	GCCGGC	1 cut(s)	687
PfoI	TCCNGGA	1 cut(s)	2049
PleI	GAGTC	6 cut(s)	7, 16, 155, 257, 718, 1848
PpsI	GAGTC	6 cut(s)	7, 16, 155, 257, 718, 1848
PpuMI	RGGWCCY	1 cut(s)	676
PshAI	GACNNNNGTC	1 cut(s)	2045
PshBI	ATTAAT	2 cut(s)	1314, 3237
PsiI	TTATAA	1 cut(s)	953
Psp124BI	GAGCTC	3 cut(s)	129, 390, 429
Psp5II	RGGWCCY	1 cut(s)	676
Psp6I	CCWGG	8 cut(s)	117, 573, 785, 908, 922, 1101, 2049, 2415
PspGI	CCWGG	8 cut(s)	117, 573, 785, 908, 922, 1101, 2049, 2415
PspPPI	RGGWCCY	1 cut(s)	676
PstI	CTGCAG	2 cut(s)	1399, 1960
PstNI	CAGNNNCTG	1 cut(s)	679
PsuI	RGATCY	4 cut(s)	1282, 1351, 1796, 2423
PvuII	CAGCTG	2 cut(s)	1330, 2195
RruI	TCGCGA	1 cut(s)	258
RsaI	GTAC	5 cut(s)	269, 515, 1380, 1474, 1527
RsaNI	GTAC	5 cut(s)	268, 514, 1379, 1473, 1526
RseI	CAYNNNNRTG	6 cut(s)	530, 974, 1293, 1775, 2662, 2925
SacI	GAGCTC	3 cut(s)	129, 390, 429
SalI	GTCGAC	1 cut(s)	10
SchI	GAGTC	6 cut(s)	7, 17, 155, 257, 719, 1848
SduI	GDGCHC	6 cut(s)	129, 390, 429, 786, 922, 1567
SexAI	ACCWGGT	1 cut(s)	2415
SfcI	CTRYAG	5 cut(s)	20, 1335, 1395, 1838, 1956
SinI	GGWCC	7 cut(s)	50, 676, 777, 1346, 2396, 2493, 3294
SmiMI	CAYNNNNRTG	6 cut(s)	530, 974, 1293, 1775, 2662, 2925
SmlI	CTYRAG	2 cut(s)	1931, 2256
SmoI	CTYRAG	2 cut(s)	1931, 2256
SpeI	ACTAGT	1 cut(s)	2042
SphI	GCATGC	1 cut(s)	1687
SseBI	AGGCCT	1 cut(s)	2789
SspI	AATATT	2 cut(s)	2359, 2756
SspMI	CTAG	1 cut(s)	2043
SstI	GAGCTC	3 cut(s)	129, 390, 429
StuI	AGGCCT	1 cut(s)	2789
StyI	CCWWGG	5 cut(s)	528, 852, 1701, 2120, 2670
TaaI	ACNGT	5 cut(s)	948, 1064, 3008, 3047, 3223
TaiI	ACGT	4 cut(s)	600, 704, 919, 3293
TaqI	TCGA	2 cut(s)	11, 2815
TaqII	GACCGA	3 cut(s)	38, 167, 794
TatI	WGTACW	3 cut(s)	513, 1472, 1525
TauI	GCSGC	4 cut(s)	209, 538, 802, 3271
TscAI	CASTG	7 cut(s)	644, 1069, 1468, 2461, 2738, 3155, 3330
TseFI	GTSAC	2 cut(s)	3002, 3062
Tsp45I	GTSAC	2 cut(s)	3002, 3062
TspRI	CASTG	7 cut(s)	644, 1069, 1468, 2461, 2738, 3155, 3330
Vha464I	CTTAAG	1 cut(s)	1931
VpaK11BI	GGWCC	7 cut(s)	50, 676, 777, 1346, 2396, 2493, 3294
VspI	ATTAAT	2 cut(s)	1314, 3237
XagI	CCTNNNNNAGG	1 cut(s)	902
XapI	RAATTY	2 cut(s)	1240, 2162
XceI	RCATGY	3 cut(s)	1439, 1687, 1837
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1123
XmiI	GTMKAC	2 cut(s)	11, 1334
XspI	CTAG	1 cut(s)	2043
Zsp2I	ATGCAT	1 cut(s)	2711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.