Rw3G029040

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000344
Physical Location & Seq
Forward (+)
44276785 .. 44286393
9609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G029040.1

Sequence Viewer

Length: 3360 bp
ATGGCCGCCGATCCAAGTCAGCAGCAGCAGCAGCAGCAGCAGCTGGCCATAATTCTTGGCCCGGACCCGAGTCAGTTCGAAACCCTAATCTCTCATCTCATGTCCTCCAGCAACGAGCAACGGTCGCAGGCCGAGGCCCTCTTCAACCTCTGCAAGCAGGCCCACCCGGACGCCCTCTTACTGAAGCTGGTCCATGTGTTGCAGGCGTCGACCCGACCCGAGTCCCGCACCATGTCCGTCATCCTCCTCCGGCGGCAGCTCACTCGCGACGACTCGTTCCTCTGGCCCCGCCTCAGCCCCTCCACTCAGTCGACCCTCAAGTCCGCACTCCTCACTTGCCTCCAATCAGAATCCACCAAATCCATGGCCAAGAAGCTCTGCGACACCGTTTCGGAGCTCGCTTCTGCCATTCTACCCGATAACCAATGGCCGGAGCTATTGCCGTTCATGTTCCACTGCGTCACCTCCGATAATTCCAAGCTCCAGGAGTCTGCGCTTTTGATTTTTGCGCAGTTGGCGCATTACATCGGCGAAACCCTAGTCCCGCAGTTGACTACGCTCCACTCAGTCTTCTACCAGTGCCTGTCCAACGCCGCCGGCAAGAGCGCCGACGTCAGAATCGCAGCACTCGGCGCCTCTGTCAATTTCATCCAGTGCCTGACCAGCGCCTCCGACAGGGACATGTTCCAGGACCTGTTGCCCTTGATGATGCAGACTTTGACGGAGGCCTTGAACGGCGGTCAGGAGGCCACGGCGCAGGAGGCGCTGGAGTTGCTGATCGAGTTGGCCGGGACCGAGCCGAGGTTCCTGCGGAGGCAATTGGTGGATGTGGTTGGCTCTATGCTTCAAATTGCGGAGGCTGCGTCCCTCGAGGAAGGTACAAGGCATTTGGCCATTGAGTTTGTCATCACTCTGGCTGAGGCCAGGGAGAGAGCCCCCGGGATGATGAGGAAGTTGCCTCAGTTTATTCATAGGTTGTTTGCCATACTTATGAACATGTTGTTGGACCTTGAGGACGACCCCGACTGGCACACTGCAGATACTGAGAATGAGGATGCTGGTGAGACTAGCAATTATGGCTTTGGACAGGAGTGTTTGGACAGACTTTCAATTTCATTGGGCGGCAATACCATTGTCCCTGTTGCTTCCGAGCTCTTTCCGTCTTACTTGGTTGCTCCTGAATGGCAAAAGCACCACGCTGTGCACATTGCCCTTGCCCAGATTGCCGAGGGGTGCTCTAAGGTAATGGTACAAAACCTCGAACAGGTGGTATCAATGGTATTGAATTCGTTCCAAGATCCTCACCCACGTGTGAGGTGGGCAGCAATTAATGCAGTTGGACAATTGTCAACGGATTTAGGCCCAAACTTACAAGAAAAGTATCACCAGCGAGTGCTGCCTGCATTAGCTGGAGCAATGGATGACTTCCAAAATCCACGAGTACAGGCCCATGCTGCTTCAGCAGTACTCAATTTCAGTGAAAACTGCACCCCAGATATTTTGGTACCGTACCTGGATGGAATTGTTAGCAAGCTTCTTGTACTTCTACGGAATGGAAAGCAGATGGTCCAGGAGGGGGCTTTAACAGCTTTAGCATCAGTTGCTGATTCGTCACAGGAGCAATTCCAGAAGTATTATGATGCTGTAATGCCTTACTTGAAAGCTATTTTGGTTAATGCAAATGACAAAGCTAATCGTATGCTACGTGCAAAATCAATGGAGTGTATTAGTTTGGTTGGAATGGCTGTCGGGAAGGATAAGTTCAGGGATGACGCAAAGCAGGTTATGGAAGTCTTGATGTCATTGCAAGGATCTGAAATGGAGGCAGATGACCCCACAACAAGTTATATGTTACAAGCATGGGCTAGACTTTGCAAATGCCTTGGGCAGGATTTTCTCCCGTACATGAATGTTGTCATGCCTTCTTTGCTTCAGTCTGCTCAGCTTAAGCCTGATGTAACAATTACTTCTGCAGATTCAGATGCAGAAATTGATGAAGATGATGATAGCATTGAAATGATTACTGTTGGCGATAAAAGGATAGGGATTAAGACTAGTGTTCTGGAGGAAAAAGCTACAGCCTGTAACATGCTATGTTGCTATGCTGATGAGTTAAAGGAAGGATTTTTCCCGTGGATTGATCAGGTTGCTCCAACATTGGTTCCTCTTCTAAAATTTTATTTCCATGAAGAAGTTAGAAAGGCAGCTGTTTCAGCGATGCCGGAGCTCCTACGCTCAGCCAAATTAGCAGTAGAGAAGGGACAATCTCAAGGTCATAATGAGTCCTATGTAAAGCAGTTATCTGATTACATAATACCAGCACTGGTTGAAGCATTACACAAGGAACCTGAGGTAGAAATTTGTGCCAGCATTCTAGATGCATTGAACGAATGTGTGCAGAGTTCTGGGCCACTTCTTGATGAAAACCAAGTTAGATGCATCGTGGATGAGATCAAACAGGTGGTTACAGCAAGCTCATCCAGAAAACAAGAGCGAGCAGAAAGGGCCAAGGCAGAGGACTTTGATGCAGAAGAAGGGGAACTTCTGAAGGAGGAAAATGAACAAGAAGAAGAGCTTTTTGACCTGGTTGGTGATTGCTTGGGAACTTTGATTAAGACATTCAAGGCATCTTTCTTGCCTTTCTTTGACGAGCTTTCCTCTTACATAACACCTATGTTGGGTAAGGATAAAACTGCAGAAGAGAGGAGAATTGCCATTTGCATATTTGATGATATTGCAGAGCATTGCCGTGAAGCTGCTCTTAAATATTATGATACTTACGTTCCTTTCCTACTGGAAGCCTGCAATGATGAAAGTTCAGATGTTCGTCAGGCTGCTGTTTATGGGGTTGGTCTTTGTGCGGAGTATGGTGGATCTGTGTTCAAACCTCTTGTTGGTGAAGCTCTTTCTAGGTTGGATATGGTGATCAGGAACCCCAATTCACAACACTCTGACAATATGATGGCATATGACAACGCTGTTTCAGCTCTTGGGAAAATATGCCAGTTCCACCGTGATAGTATAGATGCACCACAGCTTGTCCCTGCCTGGTTAGGTTGTCTGCCTATAAAAGGTGATTTGATTGAGGCCAAGGTTGTCCATGACCAGCTATGTTCAATGGTTGAAAGGTCGGATAGAGAGCTTTTGGGCCCCAATAATCAGTATCTTCCGAAAATAGTTGCGGTTTTTGCCGAGGTTATATGTGCGGGTAAAGACTTGGCCACAGAGCAAACAACTAGTCGAATGATTAATTTATTAAGGCAGCTCCAACAAACTTTACCACCCTCTACCCTTGCGTCAACCTGGTCATCCTTGCAACCCCAGCAACAGCTTGCATTGCAATTTATACTCTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

1119

Amino Acids

123.94

Weight (kDa)

4.77

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF577 PF04510 105 - 257 1.8e-07 Repeat of unknown function (DUF577)
TPR_IPO5 PF25780 119 - 278 3.8e-36 IPO5-like, TPR repeats
Importin_rep_4 PF18808 289 - 380 2.3e-22 Importin repeat
HEAT_EZ PF13513 396 - 449 4.5e-06 HEAT-like repeat
TOR1L1_N PF24714 396 - 582 2.1e-09 TORTIFOLIA1 N-terminal
HEAT_GCN1 PF23271 404 - 559 7e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EZ PF13513 479 - 533 3.4e-07 HEAT-like repeat
TPR_IMB1 PF25574 502 - 652 2.8e-11 Importin subunit beta-1-like, TPR repeats
Importin_rep_6 PF18829 788 - 894 1.9e-18 Importin repeat 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19820
fragaria_vesca FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 319
AatII GACGTC 1 cut(s) 615
AbsI CCTCGAGG 1 cut(s) 869
Acc16I TGCGCA 1 cut(s) 510
Acc36I ACCTGC 1 cut(s) 1771
Acc65I GGTACC 1 cut(s) 1504
AccB1I GGYRCC 2 cut(s) 632, 1504
AccI GTMKAC 2 cut(s) 209, 311
AccII CGCG 1 cut(s) 267
AclWI GGATC 4 cut(s) 5, 1292, 1819, 2881
AcoI YGGCCR 7 cut(s) 3, 45, 366, 428, 786, 891, 3219
AcsI RAATTY 3 cut(s) 1285, 2174, 2358
AcuI CTGAAG 4 cut(s) 203, 1443, 1916, 2567
AcvI CACGTG 1 cut(s) 1310
AcyI GRCGYC 4 cut(s) 171, 206, 612, 633
AdeI CACNNNGTG 1 cut(s) 1201
AfaI GTAC 8 cut(s) 880, 1251, 1443, 1467, 1506, 1511, 1542, 1904
AfiI CCNNNNNNNGG 9 cut(s) 430, 675, 801, 1264, 1576, 1888, 2678, 2894, 3071
AflII CTTAAG 1 cut(s) 1946
AflIII ACRYGT 3 cut(s) 681, 996, 1309
AhlI ACTAGT 2 cut(s) 2054, 3236
AjnI CCWGG 8 cut(s) 483, 687, 923, 1512, 1569, 2583, 3047, 3302
AjuI GAANNNNNNNTTGG 4 cut(s) 986, 1018, 1652, 1684
AleI CACNNNNGTG 1 cut(s) 1308
Alw21I GWGCWC 5 cut(s) 399, 1155, 1206, 1238, 2229
Alw26I GTCTC 1 cut(s) 1058
Alw44I GTGCAC 1 cut(s) 1202
AlwI GGATC 4 cut(s) 5, 1292, 1819, 2881
AlwNI CAGNNNCTG 6 cut(s) 43, 583, 658, 694, 1043, 1604
Ama87I CYCGRG 4 cut(s) 67, 218, 869, 938
ApaI GGGCCC 1 cut(s) 3152
ApaLI GTGCAC 1 cut(s) 1202
ApoI RAATTY 3 cut(s) 1285, 2174, 2358
ArsI GACNNNNNNTTYG 4 cut(s) 525, 557, 3223, 3255
AseI ATTAAT 2 cut(s) 1329, 3249
Asp700I GAANNNNTTC 1 cut(s) 1289
Asp718I GGTACC 1 cut(s) 1504
AspLEI GCGC 8 cut(s) 496, 511, 520, 608, 635, 668, 757, 766
AsuC2I CCSGG 5 cut(s) 62, 167, 790, 939, 940
AsuHPI GGTGA 8 cut(s) 454, 1073, 1295, 1376, 2603, 2909, 2935, 3086
AsuII TTCGAA 1 cut(s) 78
AvaI CYCGRG 4 cut(s) 67, 218, 869, 938
AvaII GGWCC 6 cut(s) 64, 190, 691, 792, 1006, 1567
AxyI CCTNAGG 1 cut(s) 2349
BaeGI GKGCMC 2 cut(s) 1206, 3152
BaeI ACNNNNGTAYC 2 cut(s) 2766, 2799
BalI TGGCCA 4 cut(s) 47, 368, 893, 3221
BanI GGYRCC 2 cut(s) 632, 1504
BanII GRGCYC 5 cut(s) 399, 937, 1155, 2229, 3152
BauI CACGAG 1 cut(s) 1437
BbrPI CACGTG 1 cut(s) 1310
BbsI GAAGAC 1 cut(s) 562
Bbv12I GWGCWC 5 cut(s) 399, 1155, 1206, 1238, 2229
BbvCI CCTCAGC 2 cut(s) 293, 918
BccI CCATC 3 cut(s) 1511, 1558, 2956
BceAI ACGGC 4 cut(s) 427, 751, 768, 2733
BcgI CGANNNNNNTGC 4 cut(s) 245, 279, 790, 824
BciT130I CCWGG 8 cut(s) 485, 689, 925, 1514, 1571, 2585, 3049, 3304
BclI TGATCA 2 cut(s) 2140, 2925
BcnI CCSGG 5 cut(s) 62, 167, 790, 939, 940
BcoDI GTCTC 1 cut(s) 1058
BcuI ACTAGT 2 cut(s) 2054, 3236
BfaI CTAG 7 cut(s) 539, 1068, 1866, 2055, 2375, 2910, 3237
BfmI CTRYAG 4 cut(s) 1035, 1971, 2076, 2694
BfoI RGCGCY 4 cut(s) 609, 636, 669, 767
BfrI CTTAAG 1 cut(s) 1946
BfuAI ACCTGC 1 cut(s) 1771
BlpI GCTNAGC 2 cut(s) 1941, 2236
BmcAI AGTACT 1 cut(s) 1467
Bme18I GGWCC 6 cut(s) 64, 190, 691, 792, 1006, 1567
BmeT110I CYCGRG 4 cut(s) 67, 218, 869, 938
BoxI GACNNNNGTC 3 cut(s) 69, 220, 1345
BpiI GAAGAC 1 cut(s) 562
BplI GAGNNNNNCTC 4 cut(s) 1220, 1252, 2642, 2674
BpmI CTGGAG 5 cut(s) 91, 467, 788, 1431, 2084
Bpu10I CCTNAGC 2 cut(s) 293, 918
Bpu1102I GCTNAGC 2 cut(s) 1941, 2236
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 3 cut(s) 302, 1031, 2253
BpuMI CCSGG 5 cut(s) 62, 167, 790, 939, 940
BsaAI YACGTR 2 cut(s) 1310, 1706
BsaHI GRCGYC 4 cut(s) 171, 206, 612, 633
BsaXI ACNNNNNCTCC 2 cut(s) 848, 878
Bsc4I CCNNNNNNNGG 9 cut(s) 430, 675, 801, 1264, 1576, 1888, 2678, 2894, 3071
Bse118I RCCGGY 1 cut(s) 596
Bse1I ACTGG 6 cut(s) 577, 652, 1031, 2328, 2799, 3004
Bse21I CCTNAGG 1 cut(s) 2349
Bse3DI GCAATG 6 cut(s) 1206, 1422, 1802, 2743, 2812, 3335
BseBI CCWGG 8 cut(s) 485, 689, 925, 1514, 1571, 2585, 3049, 3304
BseLI CCNNNNNNNGG 9 cut(s) 430, 675, 801, 1264, 1576, 1888, 2678, 2894, 3071
BseMI GCAATG 6 cut(s) 1206, 1422, 1802, 2743, 2812, 3335
BseMII CTCAG 9 cut(s) 307, 320, 579, 909, 974, 1035, 1955, 2250, 2340
BseNI ACTGG 6 cut(s) 577, 652, 1031, 2328, 2799, 3004
BseRI GAGGAG 3 cut(s) 236, 320, 2719
BseSI GKGCMC 2 cut(s) 1206, 3152
BseYI CCCAGC 1 cut(s) 3321
BsgI GTGCAG 2 cut(s) 1471, 2417
Bsh1236I CGCG 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 125
BshNI GGYRCC 2 cut(s) 632, 1504
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 5 cut(s) 399, 1155, 1206, 1238, 2229
BsiHKCI CYCGRG 4 cut(s) 67, 218, 869, 938
BsiSI CCGG 8 cut(s) 62, 167, 250, 431, 597, 789, 939, 2222
BslFI GGGAC 8 cut(s) 208, 527, 692, 805, 850, 1121, 2274, 3026
BslI CCNNNNNNNGG 9 cut(s) 430, 675, 801, 1264, 1576, 1888, 2678, 2894, 3071
BsmAI GTCTC 1 cut(s) 1058
BsmFI GGGAC 8 cut(s) 208, 527, 692, 805, 850, 1121, 2274, 3026
BsmI GAATGC 1 cut(s) 2370
BsoBI CYCGRG 4 cut(s) 67, 218, 869, 938
Bsp119I TTCGAA 1 cut(s) 78
Bsp120I GGGCCC 1 cut(s) 3148
Bsp1286I GDGCHC 7 cut(s) 399, 937, 1155, 1206, 1238, 2229, 3152
Bsp143I GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
Bsp1720I GCTNAGC 2 cut(s) 1941, 2236
Bsp19I CCATGG 1 cut(s) 363
Bsp68I TCGCGA 1 cut(s) 267
BspCNI CTCAG 9 cut(s) 306, 319, 578, 910, 973, 1036, 1954, 2249, 2341
BspFNI CGCG 1 cut(s) 267
BspMAI CTGCAG 3 cut(s) 1039, 1975, 2698
BspMI ACCTGC 1 cut(s) 1771
BspPI GGATC 4 cut(s) 5, 1292, 1819, 2881
BspQI GCTCTTC 1 cut(s) 2565
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 2 cut(s) 632, 1504
BspTI CTTAAG 1 cut(s) 1946
BsrDI GCAATG 6 cut(s) 1206, 1422, 1802, 2743, 2812, 3335
BsrFI RCCGGY 1 cut(s) 596
BsrI ACTGG 6 cut(s) 577, 652, 1031, 2328, 2799, 3004
BssAI RCCGGY 1 cut(s) 596
BssMI GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
BssNI GRCGYC 4 cut(s) 171, 206, 612, 633
BssSI CACGAG 1 cut(s) 1437
BssT1I CCWWGG 4 cut(s) 363, 1882, 2508, 3090
Bst2BI CACGAG 1 cut(s) 1437
Bst2UI CCWGG 8 cut(s) 485, 689, 925, 1514, 1571, 2585, 3049, 3304
Bst4CI ACNGT 5 cut(s) 123, 388, 1509, 2026, 3014
Bst6I CTCTTC 4 cut(s) 146, 2172, 2565, 2694
BstACI GRCGYC 4 cut(s) 171, 206, 612, 633
BstAFI CTTAAG 1 cut(s) 1946
BstAPI GCANNNNNTGC 2 cut(s) 1331, 1601
BstBAI YACGTR 2 cut(s) 1310, 1706
BstBI TTCGAA 1 cut(s) 78
BstDSI CCRYGG 3 cut(s) 363, 750, 2132
BstENI CCTNNNNNAGG 4 cut(s) 673, 1262, 1886, 3069
BstFNI CGCG 1 cut(s) 267
BstH2I RGCGCY 4 cut(s) 609, 636, 669, 767
BstHHI GCGC 8 cut(s) 496, 511, 520, 608, 635, 668, 757, 766
BstKTI GATC 8 cut(s) 13, 780, 1300, 1814, 2143, 2454, 2876, 2928
BstMAI GTCTC 1 cut(s) 1058
BstMBI GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
BstMCI CGRYCG 1 cut(s) 125
BstNI CCWGG 8 cut(s) 485, 689, 925, 1514, 1571, 2585, 3049, 3304
BstNSI RCATGY 3 cut(s) 685, 1000, 2092
BstPAI GACNNNNGTC 3 cut(s) 69, 220, 1345
BstSFI CTRYAG 4 cut(s) 1035, 1971, 2076, 2694
BstSLI GKGCMC 2 cut(s) 1206, 3152
BstUI CGCG 1 cut(s) 267
BstV2I GAAGAC 1 cut(s) 562
BstX2I RGATCY 3 cut(s) 1297, 1811, 2873
BstXI CCANNNNNNTGG 1 cut(s) 364
BstYI RGATCY 3 cut(s) 1297, 1811, 2873
Bsu36I CCTNAGG 1 cut(s) 2349
BtgI CCRYGG 3 cut(s) 363, 750, 2132
BtgZI GCGATG 1 cut(s) 2231
BtsI GCAGTG 2 cut(s) 454, 1032
BtsIMutI CAGTG 6 cut(s) 454, 584, 659, 1032, 1483, 2321
BtuMI TCGCGA 1 cut(s) 267
BveI ACCTGC 1 cut(s) 1771
CaiI CAGNNNCTG 6 cut(s) 43, 583, 658, 694, 1043, 1604
CfoI GCGC 8 cut(s) 496, 511, 520, 608, 635, 668, 757, 766
Cfr10I RCCGGY 1 cut(s) 596
Cfr9I CCCGGG 1 cut(s) 938
CseI GACGC 6 cut(s) 179, 195, 448, 852, 1781, 3285
CsiI ACCWGGT 2 cut(s) 2583, 3302
Csp6I GTAC 8 cut(s) 879, 1250, 1442, 1466, 1505, 1510, 1541, 1903
CviQI GTAC 8 cut(s) 879, 1250, 1442, 1466, 1505, 1510, 1541, 1903
DinI GGCGCC 1 cut(s) 634
DpnI GATC 8 cut(s) 12, 779, 1299, 1813, 2142, 2453, 2875, 2927
DpnII GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
DraIII CACNNNGTG 1 cut(s) 1201
DrdI GACNNNNNNGTC 1 cut(s) 319
DseDI GACNNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 7 cut(s) 3, 45, 366, 428, 786, 891, 3219
Eam1104I CTCTTC 4 cut(s) 146, 2172, 2565, 2694
EarI CTCTTC 4 cut(s) 146, 2172, 2565, 2694
Ecl136II GAGCTC 3 cut(s) 397, 1153, 2227
Eco130I CCWWGG 4 cut(s) 363, 1882, 2508, 3090
Eco147I AGGCCT 1 cut(s) 728
Eco24I GRGCYC 5 cut(s) 399, 937, 1155, 2229, 3152
Eco47I GGWCC 6 cut(s) 64, 190, 691, 792, 1006, 1567
Eco53kI GAGCTC 3 cut(s) 397, 1153, 2227
Eco57I CTGAAG 4 cut(s) 203, 1443, 1916, 2567
Eco72I CACGTG 1 cut(s) 1310
Eco81I CCTNAGG 1 cut(s) 2349
Eco88I CYCGRG 4 cut(s) 67, 218, 869, 938
EcoICRI GAGCTC 3 cut(s) 397, 1153, 2227
EcoNI CCTNNNNNAGG 4 cut(s) 673, 1262, 1886, 3069
EcoO109I RGGNCCY 3 cut(s) 136, 691, 3149
EcoRI GAATTC 1 cut(s) 1285
EcoRII CCWGG 8 cut(s) 483, 687, 923, 1512, 1569, 2583, 3047, 3302
EcoT14I CCWWGG 4 cut(s) 363, 1882, 2508, 3090
EcoT22I ATGCAT 2 cut(s) 2383, 2441
EcoT38I GRGCYC 5 cut(s) 399, 937, 1155, 2229, 3152
EgeI GGCGCC 1 cut(s) 634
EheI GGCGCC 1 cut(s) 634
ErhI CCWWGG 4 cut(s) 363, 1882, 2508, 3090
FalI AAGNNNNNCTT 6 cut(s) 2526, 2558, 2559, 2591, 2745, 2777
FaqI GGGAC 8 cut(s) 208, 527, 692, 805, 850, 1121, 2274, 3026
FauI CCCGC 4 cut(s) 233, 296, 552, 3199
FauNDI CATATG 1 cut(s) 2968
FbaI TGATCA 2 cut(s) 2140, 2925
FblI GTMKAC 2 cut(s) 209, 311
FriOI GRGCYC 5 cut(s) 399, 937, 1155, 2229, 3152
FspBI CTAG 7 cut(s) 539, 1068, 1866, 2055, 2375, 2910, 3237
FspI TGCGCA 1 cut(s) 510
GlaI GCGC 8 cut(s) 495, 510, 519, 607, 634, 667, 756, 765
GsaI CCCAGC 1 cut(s) 3325
GsuI CTGGAG 5 cut(s) 91, 467, 788, 1431, 2084
HaeII RGCGCY 4 cut(s) 609, 636, 669, 767
HapII CCGG 8 cut(s) 62, 167, 250, 431, 597, 789, 939, 2222
HgaI GACGC 6 cut(s) 179, 195, 448, 852, 1781, 3285
HhaI GCGC 8 cut(s) 496, 511, 520, 608, 635, 668, 757, 766
Hin1I GRCGYC 4 cut(s) 171, 206, 612, 633
Hin6I GCGC 8 cut(s) 494, 509, 518, 606, 633, 666, 755, 764
HinP1I GCGC 8 cut(s) 494, 509, 518, 606, 633, 666, 755, 764
HincII GTYRAC 5 cut(s) 210, 312, 552, 1350, 3300
HindII GTYRAC 5 cut(s) 210, 312, 552, 1350, 3300
HindIII AAGCTT 1 cut(s) 1532
HinfI GANTC 9 cut(s) 70, 221, 272, 350, 488, 618, 1607, 1976, 2282
HpaII CCGG 8 cut(s) 62, 167, 250, 431, 597, 789, 939, 2222
HphI GGTGA 8 cut(s) 454, 1073, 1295, 1376, 2603, 2909, 2935, 3086
Hpy166II GTNNAC 6 cut(s) 210, 312, 552, 1204, 1350, 3300
Hpy8I GTNNAC 6 cut(s) 210, 312, 552, 1204, 1350, 3300
Hpy99I CGWCG 3 cut(s) 211, 272, 614
HpyAV CCTTC 7 cut(s) 869, 1747, 1932, 2114, 2251, 2528, 2542
HpyCH4III ACNGT 5 cut(s) 123, 388, 1509, 2026, 3014
HpyCH4IV ACGT 4 cut(s) 612, 1309, 1705, 2781
HpySE526I ACGT 4 cut(s) 612, 1309, 1705, 2781
Hsp92I GRCGYC 4 cut(s) 171, 206, 612, 633
HspAI GCGC 8 cut(s) 494, 509, 518, 606, 633, 666, 755, 764
KasI GGCGCC 1 cut(s) 632
KpnI GGTACC 1 cut(s) 1508
KroI GCCGGC 1 cut(s) 596
KroNI GCCGGC 1 cut(s) 598
Ksp22I TGATCA 2 cut(s) 2140, 2925
Kzo9I GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
LguI GCTCTTC 1 cut(s) 2565
MabI ACCWGGT 2 cut(s) 2583, 3302
MaeI CTAG 7 cut(s) 539, 1068, 1866, 2055, 2375, 2910, 3237
MaeII ACGT 4 cut(s) 612, 1309, 1705, 2781
MaeIII GTNAC 6 cut(s) 460, 1611, 1851, 1957, 2084, 2464
MalI GATC 8 cut(s) 12, 779, 1299, 1813, 2142, 2453, 2875, 2927
MboI GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
MfeI CAATTG 2 cut(s) 818, 1343
MflI RGATCY 3 cut(s) 1297, 1811, 2873
MhlI GDGCHC 7 cut(s) 399, 937, 1155, 1206, 1238, 2229, 3152
MlsI TGGCCA 4 cut(s) 47, 368, 893, 3221
MluNI TGGCCA 4 cut(s) 47, 368, 893, 3221
Mly113I GGCGCC 1 cut(s) 633
MlyI GAGTC 5 cut(s) 79, 230, 266, 497, 2291
MmeI TCCRAC 9 cut(s) 612, 696, 984, 1318, 1717, 2177, 2895, 3111, 3292
Mox20I TGGCCA 4 cut(s) 47, 368, 893, 3221
Mph1103I ATGCAT 2 cut(s) 2383, 2441
MroNI GCCGGC 1 cut(s) 596
MroXI GAANNNNTTC 1 cut(s) 1289
MscI TGGCCA 4 cut(s) 47, 368, 893, 3221
MslI CAYNNNNRTG 4 cut(s) 989, 1308, 2015, 2748
Msp20I TGGCCA 4 cut(s) 47, 368, 893, 3221
MspA1I CMGCKG 2 cut(s) 43, 2207
MspCI CTTAAG 1 cut(s) 1946
MspI CCGG 8 cut(s) 62, 167, 250, 431, 597, 789, 939, 2222
MunI CAATTG 2 cut(s) 818, 1343
Mva1269I GAATGC 1 cut(s) 2370
MvaI CCWGG 8 cut(s) 485, 689, 925, 1514, 1571, 2585, 3049, 3304
MvnI CGCG 1 cut(s) 267
NaeI GCCGGC 1 cut(s) 598
NarI GGCGCC 1 cut(s) 633
NciI CCSGG 5 cut(s) 62, 167, 790, 939, 940
NcoI CCATGG 1 cut(s) 363
NdeI CATATG 1 cut(s) 2968
NdeII GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
NgoMIV GCCGGC 1 cut(s) 596
NmeAIII GCCGAG 5 cut(s) 157, 609, 825, 1252, 3217
NmuCI GTSAC 2 cut(s) 460, 1611
NruI TCGCGA 1 cut(s) 267
NsbI TGCGCA 1 cut(s) 510
NsiI ATGCAT 2 cut(s) 2383, 2441
NspI RCATGY 3 cut(s) 685, 1000, 2092
NspV TTCGAA 1 cut(s) 78
OliI CACNNNNGTG 1 cut(s) 1308
PaeR7I CTCGAG 1 cut(s) 869
PceI AGGCCT 1 cut(s) 728
PciI ACATGT 2 cut(s) 681, 996
PciSI GCTCTTC 1 cut(s) 2565
PcsI WCGNNNNNNNCGW 1 cut(s) 1702
PctI GAATGC 1 cut(s) 2370
PdiI GCCGGC 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 1289
PfeI GAWTC 4 cut(s) 350, 618, 1607, 1976
PfoI TCCNGGA 3 cut(s) 483, 687, 1569
PleI GAGTC 5 cut(s) 78, 229, 266, 496, 2290
PluTI GGCGCC 1 cut(s) 636
PmaCI CACGTG 1 cut(s) 1310
PmlI CACGTG 1 cut(s) 1310
PpsI GAGTC 5 cut(s) 78, 229, 266, 496, 2290
Ppu21I YACGTR 2 cut(s) 1310, 1706
PpuMI RGGWCCY 1 cut(s) 691
PscI ACATGT 2 cut(s) 681, 996
PshAI GACNNNNGTC 3 cut(s) 69, 220, 1345
PshBI ATTAAT 2 cut(s) 1329, 3249
Psp124BI GAGCTC 3 cut(s) 399, 1155, 2229
Psp5II RGGWCCY 1 cut(s) 691
Psp6I CCWGG 8 cut(s) 483, 687, 923, 1512, 1569, 2583, 3047, 3302
PspCI CACGTG 1 cut(s) 1310
PspFI CCCAGC 1 cut(s) 3321
PspGI CCWGG 8 cut(s) 483, 687, 923, 1512, 1569, 2583, 3047, 3302
PspOMI GGGCCC 1 cut(s) 3148
PspPPI RGGWCCY 1 cut(s) 691
PspXI VCTCGAGB 1 cut(s) 869
PstI CTGCAG 3 cut(s) 1039, 1975, 2698
PstNI CAGNNNCTG 6 cut(s) 43, 583, 658, 694, 1043, 1604
PsuI RGATCY 3 cut(s) 1297, 1811, 2873
PvuII CAGCTG 2 cut(s) 43, 2207
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 8 cut(s) 880, 1251, 1443, 1467, 1506, 1511, 1542, 1904
RsaNI GTAC 8 cut(s) 879, 1250, 1442, 1466, 1505, 1510, 1541, 1903
RseI CAYNNNNRTG 4 cut(s) 989, 1308, 2015, 2748
SacI GAGCTC 3 cut(s) 399, 1155, 2229
SalI GTCGAC 2 cut(s) 208, 310
SapI GCTCTTC 1 cut(s) 2565
Sau3AI GATC 8 cut(s) 10, 777, 1297, 1811, 2140, 2451, 2873, 2925
ScaI AGTACT 1 cut(s) 1467
SchI GAGTC 5 cut(s) 79, 230, 266, 497, 2291
SduI GDGCHC 7 cut(s) 399, 937, 1155, 1206, 1238, 2229, 3152
SexAI ACCWGGT 2 cut(s) 2583, 3302
SfcI CTRYAG 4 cut(s) 1035, 1971, 2076, 2694
SfoI GGCGCC 1 cut(s) 634
Sfr274I CTCGAG 1 cut(s) 869
SfuI TTCGAA 1 cut(s) 78
SinI GGWCC 6 cut(s) 64, 190, 691, 792, 1006, 1567
SlaI CTCGAG 1 cut(s) 869
SmaI CCCGGG 1 cut(s) 940
SmiMI CAYNNNNRTG 4 cut(s) 989, 1308, 2015, 2748
SmlI CTYRAG 5 cut(s) 317, 869, 1010, 1946, 2268
SmoI CTYRAG 5 cut(s) 317, 869, 1010, 1946, 2268
SpeI ACTAGT 2 cut(s) 2054, 3236
SseBI AGGCCT 1 cut(s) 728
SspDI GGCGCC 1 cut(s) 632
SspI AATATT 1 cut(s) 2768
SspMI CTAG 7 cut(s) 539, 1068, 1866, 2055, 2375, 2910, 3237
SstI GAGCTC 3 cut(s) 399, 1155, 2229
StuI AGGCCT 1 cut(s) 728
StyI CCWWGG 4 cut(s) 363, 1882, 2508, 3090
TaaI ACNGT 5 cut(s) 123, 388, 1509, 2026, 3014
TaiI ACGT 4 cut(s) 615, 1312, 1708, 2784
TaqI TCGA 7 cut(s) 78, 209, 311, 780, 870, 1260, 3241
TaqII GACCGA 1 cut(s) 809
TatI WGTACW 3 cut(s) 1441, 1465, 1540
TauI GCSGC 4 cut(s) 8, 256, 596, 1125
TfiI GAWTC 4 cut(s) 350, 618, 1607, 1976
TscAI CASTG 6 cut(s) 461, 584, 659, 1039, 1483, 2328
TseFI GTSAC 2 cut(s) 460, 1611
Tsp45I GTSAC 2 cut(s) 460, 1611
TspGWI ACGGA 5 cut(s) 226, 737, 1149, 1367, 1564
TspMI CCCGGG 1 cut(s) 938
TspRI CASTG 6 cut(s) 461, 584, 659, 1039, 1483, 2328
Vha464I CTTAAG 1 cut(s) 1946
VneI GTGCAC 1 cut(s) 1202
VpaK11BI GGWCC 6 cut(s) 64, 190, 691, 792, 1006, 1567
VspI ATTAAT 2 cut(s) 1329, 3249
XagI CCTNNNNNAGG 4 cut(s) 673, 1262, 1886, 3069
XapI RAATTY 3 cut(s) 1285, 2174, 2358
XbaI TCTAGA 1 cut(s) 2374
XceI RCATGY 3 cut(s) 685, 1000, 2092
XcmI CCANNNNNNNNNTGG 2 cut(s) 361, 1314
XhoI CTCGAG 1 cut(s) 869
XmaI CCCGGG 1 cut(s) 938
XmiI GTMKAC 2 cut(s) 209, 311
XmnI GAANNNNTTC 1 cut(s) 1289
XspI CTAG 7 cut(s) 539, 1068, 1866, 2055, 2375, 2910, 3237
ZraI GACGTC 1 cut(s) 613
ZrmI AGTACT 1 cut(s) 1467
Zsp2I ATGCAT 2 cut(s) 2383, 2441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.