Rh3CG364700

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000344
Physical Location & Seq
Forward (+)
45768474 .. 45778427
9954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG364700.1

Sequence Viewer

Length: 3351 bp
ATGGCGGCCGATCCAAGTCAGCAGCAGCAGCAGCTGGCCATAATTCTTGGCCCGGACCCGAGTCAGTTCGAAACCCTAATCTCTCATCTCATGTCCTCCAGCAACGAGCAACGGTCGCAGGCCGAGGCCCTCTTCAACCTCTGCAAGCAGGCCCACCCGGACGCCCTCTTACTGAAGCTGGTCCATGTGTTGCAGGCGTCGACCCGACCCGAGTCCCGCACCATGTCCGTCATCCTCCTCCGGCGGCAGCTCACTCGCGACGACTCGTTCCTCTGGCCCCGCCTCAGCCCCTCCACTCAGTCGACCCTCAAGTCCGCACTCCTCACTTGCCTCCAATCAGAATCCACCAAATCCATGGCCAAGAAGCTCTGCGACACCGTTTCGGAGCTCGCTTCTGCCATTCTACCCGATAACCAATGGCCGGAGCTATTGCCGTTCATGTTCCACTGCGTCACCTCCGATAATTCCAAGCTCCAGGAGTCTGCGCTTTTGATTTTTGCGCAGTTGGCGCATTACATCGGCGAAACCCTAGTCCCGCAGTTGACTACGCTCCACTCAGTCTTCTACCAGTGCCTGTCCAACGCCGCCGGCAAGAGCGCCGACGTCAGAATCGCAGCACTCGGCGCCTCTGTCAATTTCATCCAGTGCCTGACCAGCGCCTCCGACAGGGACAGGTTCCAGGACCTGTTGCCCTTGATGATGCAGACTTTGACGGAGGCCTTGAACGGCGGTCAGGAGGCCACGGCCCAGGAGGCGCTGGAGTTGCTGATCGAGTTGGCCGGGACCGAGCCGAGGTTCCTGCGGAGGCAATTGGTGGATGTGGTTGGCTCTATGCTTCAAATTGCGGAGGCTGCGTCCCTCGAGGAAGGTACAAGGCATTTGGCCATTGAGTTTGTCATCACTCTGGCTGAGGCCAGGGAGAGAGCTCCCGGGATGATGAGGAAGTTGCCTCAGTTTATTCATAGGTTGTTTGCCATACTTATGAACATGTTGTTGGACCTTGAGGACGACCCCGACTGGCACACTGCAGATACTGAGAATGAGGATGCTGGTGAGACTAGCAATTATGGCTTTGGACAGGAGTGTTTGGACAGACTTTCAATTTCATTGGGCGGCAATACCATTGTCCCTGTTGCTTCCGAGCTCTTTCCGTCTTACTTGGTTGCTCCTGAATGGCAAAAGCACCACGCTGTGCACATTGCCCTTGCCCAGATTGCCGAGGGGTGCTCTAAGGTAATGGTACAAAACCTCGAACAGGTGGTATCAATGGTATTGAATTCGTTCCAAGATCCTCACCCACGTGTGAGGTGGGCAGCAATTAATGCAGTTGGACAATTGTCAACGGATTTAGGCCCAAACTTACAAGAAAAGTATCACCAGCGAGTGCTGCCTGCATTAGCTGGAGCAATGGATGACTTCCAAAATCCACGAGTACAGGCCCATGCTGCTTCAGCAGTACTCAATTTCAGTGAAAACTGCACCCCAGATATTTTGGTACCGTACCTGGATGGAATTGTTAGCAAGCTTCTTGTACTTCTACGGAATGGAAAGCAGATGGTCCAGGAGGGGGCTTTAACAGCTTTAGCATCAGTTGCTGATTCGTCACAGGAGCAATTCCAGAAGTATTATGATGCTGTAATGCCTTACTTGAAAGCTATTTTGGTTAATGCAAATGACAAAGCTAATCGTATGCTACGTGCAAAATCAATGGAGTGTATTAGTTTGGTTGGAATGGCTGTCGGGAAGGATAAGTTCAGGGATGACGCAAAGCAGGTTATGGAAGTCTTGATGTCATTGCAAGGATCTGAAATGGAGGCAGATGACCCCACAACAAGTTATATGTTACAAGCATGGGCTAGACTTTGCAAATGCCTTGGGCAGGATTTTCTCCCGTACATGAATGTTGTCATGCCTTCTTTGCTTCAGTCTGCTCAGCTTAAGCCTGATGTAACAATTACTTCTGCAGATTCAGATGCTGAAATTGATGAAGATGATGATAGCATTGAAATGATTACTGTTGGCGATAAAAGGATAGGGATTAAGACTAGTGTTCTGGAGGAAAAAGCTACAGCCTGTAACATGCTATGTTGCTATGCTGATGAGTTAAAGGAAGGATTTTTCCCGTGGATTGATCAGGTTGCTCCAACATTGGTTCCTCTTCTAAAATTTTATTTCCATGAAGAAGTTAGAAAGGCAGCTGTTTCAGCGATGCCGGAGCTCCTACGCTCAGCCAAATTAGCAGTAGAGAAGGGACAATCTCAAGGTCATAATGAGTCCTATGTAAAGCAGTTATCTGATTACATAATACCAGCACTGGTTGAAGCATTACACAAGGAACCTGAGGTAGAAATTTGTGCCAGCATTCTAGATGCATTGAACGAATGTGTGCAGAGTTCTGGGCCACTTCTTGATGAAAACCAAGTTAGATGCATCGTGGATGAGATCAAACAGGTGGTTACAGCAAGCTCATCCAGAAAACAAGAGCGAGCAGAAAGGGCCAAGGCAGAGGACTTTGATGCAGAAGAAGGGGAACTTCTGAAGGAGGAAAATGAACAAGAAGAAGAGCTTTTTGACCTGGTTGGTGATTGCTTGGGAACTTTGATTAAGACATTCAAGGCATCTTTCTTGCCTTTCTTTGACGAGCTTTCCTCTTACATAACACCTATGTTGGGTAAGGATAAAACTGCAGAAGAGAGGAGAATTGCCATTTGCATATTTGATGATATTGCAGAGCATTGCCGTGAAGCTGCTCTTAAATATTATGATACTTACGTTCCTTTCCTACTGGAAGCCTGCAATGATGAAAGTTCAGATGTTCGTCAGGCTGCTGTTTATGGGGTTGGTCTTTGTGCGGAGTATGGTGGATCTGTGTTCAAACCTCTTGTTGGTGAAGCTCTTTCTAGGTTGGATATGGTGATCAGGAACCCCAATTCACAACACTCTGACAATATGATGGCATATGACAACGCTGTTTCAGCTCTTGGGAAAATATGCCAGTTCCACCGTGATAGTATAGATGCACCACAGCTTGTCCCTGCCTGGTTAGGTTGTCTGCCTATAAAAGGTGATTTGATTGAGGCCAAGGTTGTCCATGACCAGCTATGTTCAATGGTTGAAAGGTCGGATAGAGAGCTTTTGGGCCCCAATAATCAGTATCTTCCGAAAATAGTTGCGGTTTTTGCTGAGGTTATATGTGCGGGTAAAGACTTGGCCACAGAGCAAACAACTAGTCGAATGATTAATTTATTAAGGCAGCTCCAACAAACTTTACCACCCTCTACCCTTGCGTCAACCTGGTCATCCTTGCAACCCCAGCAACAGCTTGCATTGCAATTTATACTCTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

1116

Amino Acids

123.58

Weight (kDa)

4.78

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF577 PF04510 102 - 254 7.6e-07 Repeat of unknown function (DUF577)
TPR_IPO5 PF25780 116 - 275 2.2e-36 IPO5-like, TPR repeats
Importin_rep_4 PF18808 286 - 377 2.3e-22 Importin repeat
HEAT_EZ PF13513 393 - 446 4.5e-06 HEAT-like repeat
TOR1L1_N PF24714 393 - 579 2.1e-09 TORTIFOLIA1 N-terminal
HEAT_GCN1 PF23271 401 - 556 7e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EZ PF13513 476 - 530 3.4e-07 HEAT-like repeat
TPR_IMB1 PF25574 499 - 649 2.8e-11 Importin subunit beta-1-like, TPR repeats
Importin_rep_6 PF18829 785 - 891 1.9e-18 Importin repeat 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19820
fragaria_vesca FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 310
AatII GACGTC 1 cut(s) 606
AbsI CCTCGAGG 1 cut(s) 860
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 1762
Acc65I GGTACC 1 cut(s) 1495
AccB1I GGYRCC 2 cut(s) 623, 1495
AccI GTMKAC 2 cut(s) 200, 302
AccII CGCG 1 cut(s) 258
AclWI GGATC 4 cut(s) 5, 1283, 1810, 2872
AcoI YGGCCR 7 cut(s) 6, 36, 357, 419, 777, 882, 3210
AcsI RAATTY 3 cut(s) 1276, 2165, 2349
AcuI CTGAAG 4 cut(s) 194, 1434, 1907, 2558
AcvI CACGTG 1 cut(s) 1301
AcyI GRCGYC 4 cut(s) 162, 197, 603, 624
AdeI CACNNNGTG 1 cut(s) 1192
AfaI GTAC 8 cut(s) 871, 1242, 1434, 1458, 1497, 1502, 1533, 1895
AfiI CCNNNNNNNGG 9 cut(s) 421, 666, 792, 1255, 1567, 1879, 2669, 2885, 3062
AflII CTTAAG 1 cut(s) 1937
AflIII ACRYGT 2 cut(s) 987, 1300
AhlI ACTAGT 2 cut(s) 2045, 3227
AjnI CCWGG 9 cut(s) 474, 678, 747, 914, 1503, 1560, 2574, 3038, 3293
AjuI GAANNNNNNNTTGG 4 cut(s) 977, 1009, 1643, 1675
AleI CACNNNNGTG 1 cut(s) 1299
Alw21I GWGCWC 6 cut(s) 390, 928, 1146, 1197, 1229, 2220
Alw26I GTCTC 1 cut(s) 1049
Alw44I GTGCAC 1 cut(s) 1193
AlwI GGATC 4 cut(s) 5, 1283, 1810, 2872
AlwNI CAGNNNCTG 7 cut(s) 34, 574, 649, 685, 1034, 1595, 1976
Ama87I CYCGRG 4 cut(s) 58, 209, 860, 929
ApaI GGGCCC 1 cut(s) 3143
ApaLI GTGCAC 1 cut(s) 1193
ApoI RAATTY 3 cut(s) 1276, 2165, 2349
ArsI GACNNNNNNTTYG 4 cut(s) 516, 548, 3214, 3246
AseI ATTAAT 2 cut(s) 1320, 3240
Asp700I GAANNNNTTC 1 cut(s) 1280
Asp718I GGTACC 1 cut(s) 1495
AspLEI GCGC 7 cut(s) 487, 502, 511, 599, 626, 659, 757
AsuC2I CCSGG 5 cut(s) 53, 158, 781, 930, 931
AsuHPI GGTGA 8 cut(s) 445, 1064, 1286, 1367, 2594, 2900, 2926, 3077
AsuII TTCGAA 1 cut(s) 69
AvaI CYCGRG 4 cut(s) 58, 209, 860, 929
AvaII GGWCC 6 cut(s) 55, 181, 682, 783, 997, 1558
AxyI CCTNAGG 1 cut(s) 2340
BaeGI GKGCMC 2 cut(s) 1197, 3143
BaeI ACNNNNGTAYC 2 cut(s) 2757, 2790
BalI TGGCCA 4 cut(s) 38, 359, 884, 3212
BanI GGYRCC 2 cut(s) 623, 1495
BanII GRGCYC 5 cut(s) 390, 928, 1146, 2220, 3143
BauI CACGAG 1 cut(s) 1428
BbrPI CACGTG 1 cut(s) 1301
BbsI GAAGAC 1 cut(s) 553
Bbv12I GWGCWC 6 cut(s) 390, 928, 1146, 1197, 1229, 2220
BbvCI CCTCAGC 3 cut(s) 284, 909, 3183
BccI CCATC 3 cut(s) 1502, 1549, 2947
BceAI ACGGC 4 cut(s) 418, 742, 759, 2724
BcgI CGANNNNNNTGC 4 cut(s) 236, 270, 781, 815
BciT130I CCWGG 9 cut(s) 476, 680, 749, 916, 1505, 1562, 2576, 3040, 3295
BclI TGATCA 2 cut(s) 2131, 2916
BcnI CCSGG 5 cut(s) 53, 158, 781, 930, 931
BcoDI GTCTC 1 cut(s) 1049
BcuI ACTAGT 2 cut(s) 2045, 3227
BfaI CTAG 7 cut(s) 530, 1059, 1857, 2046, 2366, 2901, 3228
BfmI CTRYAG 4 cut(s) 1026, 1962, 2067, 2685
BfoI RGCGCY 4 cut(s) 600, 627, 660, 758
BfrI CTTAAG 1 cut(s) 1937
BfuAI ACCTGC 1 cut(s) 1762
BglI GCCNNNNNGGC 1 cut(s) 752
BlpI GCTNAGC 2 cut(s) 1932, 2227
BmcAI AGTACT 1 cut(s) 1458
Bme18I GGWCC 6 cut(s) 55, 181, 682, 783, 997, 1558
BmeT110I CYCGRG 4 cut(s) 58, 209, 860, 929
BoxI GACNNNNGTC 3 cut(s) 60, 211, 1336
BpiI GAAGAC 1 cut(s) 553
BplI GAGNNNNNCTC 4 cut(s) 1211, 1243, 2633, 2665
BpmI CTGGAG 5 cut(s) 82, 458, 779, 1422, 2075
Bpu10I CCTNAGC 3 cut(s) 284, 909, 3183
Bpu1102I GCTNAGC 2 cut(s) 1932, 2227
Bpu14I TTCGAA 1 cut(s) 69
BpuEI CTTGAG 3 cut(s) 293, 1022, 2244
BpuMI CCSGG 5 cut(s) 53, 158, 781, 930, 931
BsaAI YACGTR 2 cut(s) 1301, 1697
BsaHI GRCGYC 4 cut(s) 162, 197, 603, 624
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 9 cut(s) 421, 666, 792, 1255, 1567, 1879, 2669, 2885, 3062
Bse118I RCCGGY 1 cut(s) 587
Bse1I ACTGG 6 cut(s) 568, 643, 1022, 2319, 2790, 2995
Bse21I CCTNAGG 1 cut(s) 2340
Bse3DI GCAATG 6 cut(s) 1197, 1413, 1793, 2734, 2803, 3326
BseBI CCWGG 9 cut(s) 476, 680, 749, 916, 1505, 1562, 2576, 3040, 3295
BseLI CCNNNNNNNGG 9 cut(s) 421, 666, 792, 1255, 1567, 1879, 2669, 2885, 3062
BseMI GCAATG 6 cut(s) 1197, 1413, 1793, 2734, 2803, 3326
BseNI ACTGG 6 cut(s) 568, 643, 1022, 2319, 2790, 2995
BseRI GAGGAG 3 cut(s) 227, 311, 2710
BseSI GKGCMC 2 cut(s) 1197, 3143
BseX3I CGGCCG 1 cut(s) 6
BseYI CCCAGC 1 cut(s) 3312
BsgI GTGCAG 2 cut(s) 1462, 2408
Bsh1236I CGCG 1 cut(s) 258
Bsh1285I CGRYCG 2 cut(s) 9, 116
BshNI GGYRCC 2 cut(s) 623, 1495
BsiEI CGRYCG 2 cut(s) 9, 116
BsiHKAI GWGCWC 6 cut(s) 390, 928, 1146, 1197, 1229, 2220
BsiHKCI CYCGRG 4 cut(s) 58, 209, 860, 929
BsiSI CCGG 8 cut(s) 53, 158, 241, 422, 588, 780, 930, 2213
BslFI GGGAC 8 cut(s) 199, 518, 683, 796, 841, 1112, 2265, 3017
BslI CCNNNNNNNGG 9 cut(s) 421, 666, 792, 1255, 1567, 1879, 2669, 2885, 3062
BsmAI GTCTC 1 cut(s) 1049
BsmFI GGGAC 8 cut(s) 199, 518, 683, 796, 841, 1112, 2265, 3017
BsmI GAATGC 1 cut(s) 2361
BsoBI CYCGRG 4 cut(s) 58, 209, 860, 929
Bsp119I TTCGAA 1 cut(s) 69
Bsp120I GGGCCC 1 cut(s) 3139
Bsp1286I GDGCHC 7 cut(s) 390, 928, 1146, 1197, 1229, 2220, 3143
Bsp143I GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
Bsp1720I GCTNAGC 2 cut(s) 1932, 2227
Bsp19I CCATGG 1 cut(s) 354
Bsp68I TCGCGA 1 cut(s) 258
BspFNI CGCG 1 cut(s) 258
BspMAI CTGCAG 3 cut(s) 1030, 1966, 2689
BspMI ACCTGC 1 cut(s) 1762
BspPI GGATC 4 cut(s) 5, 1283, 1810, 2872
BspQI GCTCTTC 1 cut(s) 2556
BspT104I TTCGAA 1 cut(s) 69
BspT107I GGYRCC 2 cut(s) 623, 1495
BspTI CTTAAG 1 cut(s) 1937
BsrDI GCAATG 6 cut(s) 1197, 1413, 1793, 2734, 2803, 3326
BsrFI RCCGGY 1 cut(s) 587
BsrI ACTGG 6 cut(s) 568, 643, 1022, 2319, 2790, 2995
BssAI RCCGGY 1 cut(s) 587
BssMI GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
BssNI GRCGYC 4 cut(s) 162, 197, 603, 624
BssSI CACGAG 1 cut(s) 1428
BssT1I CCWWGG 4 cut(s) 354, 1873, 2499, 3081
Bst2BI CACGAG 1 cut(s) 1428
Bst2UI CCWGG 9 cut(s) 476, 680, 749, 916, 1505, 1562, 2576, 3040, 3295
Bst4CI ACNGT 5 cut(s) 114, 379, 1500, 2017, 3005
Bst6I CTCTTC 4 cut(s) 137, 2163, 2556, 2685
BstACI GRCGYC 4 cut(s) 162, 197, 603, 624
BstAFI CTTAAG 1 cut(s) 1937
BstAPI GCANNNNNTGC 2 cut(s) 1322, 1592
BstBAI YACGTR 2 cut(s) 1301, 1697
BstBI TTCGAA 1 cut(s) 69
BstDSI CCRYGG 3 cut(s) 354, 741, 2123
BstENI CCTNNNNNAGG 4 cut(s) 664, 1253, 1877, 3060
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 4 cut(s) 600, 627, 660, 758
BstHHI GCGC 7 cut(s) 487, 502, 511, 599, 626, 659, 757
BstKTI GATC 8 cut(s) 13, 771, 1291, 1805, 2134, 2445, 2867, 2919
BstMAI GTCTC 1 cut(s) 1049
BstMBI GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
BstMCI CGRYCG 2 cut(s) 9, 116
BstNI CCWGG 9 cut(s) 476, 680, 749, 916, 1505, 1562, 2576, 3040, 3295
BstNSI RCATGY 2 cut(s) 991, 2083
BstPAI GACNNNNGTC 3 cut(s) 60, 211, 1336
BstSFI CTRYAG 4 cut(s) 1026, 1962, 2067, 2685
BstSLI GKGCMC 2 cut(s) 1197, 3143
BstUI CGCG 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 553
BstX2I RGATCY 3 cut(s) 1288, 1802, 2864
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 3 cut(s) 1288, 1802, 2864
BstZI CGGCCG 1 cut(s) 6
Bsu36I CCTNAGG 1 cut(s) 2340
BtgI CCRYGG 3 cut(s) 354, 741, 2123
BtgZI GCGATG 1 cut(s) 2222
BtsI GCAGTG 2 cut(s) 445, 1023
BtsIMutI CAGTG 6 cut(s) 445, 575, 650, 1023, 1474, 2312
BtuMI TCGCGA 1 cut(s) 258
BveI ACCTGC 1 cut(s) 1762
CaiI CAGNNNCTG 7 cut(s) 34, 574, 649, 685, 1034, 1595, 1976
CfoI GCGC 7 cut(s) 487, 502, 511, 599, 626, 659, 757
Cfr10I RCCGGY 1 cut(s) 587
Cfr9I CCCGGG 1 cut(s) 929
CseI GACGC 6 cut(s) 170, 186, 439, 843, 1772, 3276
CsiI ACCWGGT 2 cut(s) 2574, 3293
Csp6I GTAC 8 cut(s) 870, 1241, 1433, 1457, 1496, 1501, 1532, 1894
CviQI GTAC 8 cut(s) 870, 1241, 1433, 1457, 1496, 1501, 1532, 1894
DinI GGCGCC 1 cut(s) 625
DpnI GATC 8 cut(s) 12, 770, 1290, 1804, 2133, 2444, 2866, 2918
DpnII GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
DraIII CACNNNGTG 1 cut(s) 1192
DrdI GACNNNNNNGTC 1 cut(s) 310
DseDI GACNNNNNNGTC 1 cut(s) 310
EaeI YGGCCR 7 cut(s) 6, 36, 357, 419, 777, 882, 3210
EagI CGGCCG 1 cut(s) 6
Eam1104I CTCTTC 4 cut(s) 137, 2163, 2556, 2685
EarI CTCTTC 4 cut(s) 137, 2163, 2556, 2685
Ecl136II GAGCTC 4 cut(s) 388, 926, 1144, 2218
EclXI CGGCCG 1 cut(s) 6
Eco130I CCWWGG 4 cut(s) 354, 1873, 2499, 3081
Eco147I AGGCCT 1 cut(s) 719
Eco24I GRGCYC 5 cut(s) 390, 928, 1146, 2220, 3143
Eco47I GGWCC 6 cut(s) 55, 181, 682, 783, 997, 1558
Eco52I CGGCCG 1 cut(s) 6
Eco53kI GAGCTC 4 cut(s) 388, 926, 1144, 2218
Eco57I CTGAAG 4 cut(s) 194, 1434, 1907, 2558
Eco72I CACGTG 1 cut(s) 1301
Eco81I CCTNAGG 1 cut(s) 2340
Eco88I CYCGRG 4 cut(s) 58, 209, 860, 929
EcoICRI GAGCTC 4 cut(s) 388, 926, 1144, 2218
EcoNI CCTNNNNNAGG 4 cut(s) 664, 1253, 1877, 3060
EcoO109I RGGNCCY 3 cut(s) 127, 682, 3140
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 9 cut(s) 474, 678, 747, 914, 1503, 1560, 2574, 3038, 3293
EcoT14I CCWWGG 4 cut(s) 354, 1873, 2499, 3081
EcoT22I ATGCAT 2 cut(s) 2374, 2432
EcoT38I GRGCYC 5 cut(s) 390, 928, 1146, 2220, 3143
EgeI GGCGCC 1 cut(s) 625
EheI GGCGCC 1 cut(s) 625
ErhI CCWWGG 4 cut(s) 354, 1873, 2499, 3081
FalI AAGNNNNNCTT 6 cut(s) 2517, 2549, 2550, 2582, 2736, 2768
FaqI GGGAC 8 cut(s) 199, 518, 683, 796, 841, 1112, 2265, 3017
FauI CCCGC 4 cut(s) 224, 287, 543, 3190
FauNDI CATATG 1 cut(s) 2959
FbaI TGATCA 2 cut(s) 2131, 2916
FblI GTMKAC 2 cut(s) 200, 302
FriOI GRGCYC 5 cut(s) 390, 928, 1146, 2220, 3143
FspBI CTAG 7 cut(s) 530, 1059, 1857, 2046, 2366, 2901, 3228
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 7 cut(s) 486, 501, 510, 598, 625, 658, 756
GsaI CCCAGC 1 cut(s) 3316
GsuI CTGGAG 5 cut(s) 82, 458, 779, 1422, 2075
HaeII RGCGCY 4 cut(s) 600, 627, 660, 758
HapII CCGG 8 cut(s) 53, 158, 241, 422, 588, 780, 930, 2213
HgaI GACGC 6 cut(s) 170, 186, 439, 843, 1772, 3276
HhaI GCGC 7 cut(s) 487, 502, 511, 599, 626, 659, 757
Hin1I GRCGYC 4 cut(s) 162, 197, 603, 624
Hin6I GCGC 7 cut(s) 485, 500, 509, 597, 624, 657, 755
HinP1I GCGC 7 cut(s) 485, 500, 509, 597, 624, 657, 755
HincII GTYRAC 5 cut(s) 201, 303, 543, 1341, 3291
HindII GTYRAC 5 cut(s) 201, 303, 543, 1341, 3291
HindIII AAGCTT 1 cut(s) 1523
HinfI GANTC 9 cut(s) 61, 212, 263, 341, 479, 609, 1598, 1967, 2273
HpaII CCGG 8 cut(s) 53, 158, 241, 422, 588, 780, 930, 2213
HphI GGTGA 8 cut(s) 445, 1064, 1286, 1367, 2594, 2900, 2926, 3077
Hpy166II GTNNAC 6 cut(s) 201, 303, 543, 1195, 1341, 3291
Hpy8I GTNNAC 6 cut(s) 201, 303, 543, 1195, 1341, 3291
Hpy99I CGWCG 3 cut(s) 202, 263, 605
HpyAV CCTTC 7 cut(s) 860, 1738, 1923, 2105, 2242, 2519, 2533
HpyCH4III ACNGT 5 cut(s) 114, 379, 1500, 2017, 3005
HpyCH4IV ACGT 4 cut(s) 603, 1300, 1696, 2772
HpySE526I ACGT 4 cut(s) 603, 1300, 1696, 2772
Hsp92I GRCGYC 4 cut(s) 162, 197, 603, 624
HspAI GCGC 7 cut(s) 485, 500, 509, 597, 624, 657, 755
KasI GGCGCC 1 cut(s) 623
KpnI GGTACC 1 cut(s) 1499
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Ksp22I TGATCA 2 cut(s) 2131, 2916
Kzo9I GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
LguI GCTCTTC 1 cut(s) 2556
MabI ACCWGGT 2 cut(s) 2574, 3293
MaeI CTAG 7 cut(s) 530, 1059, 1857, 2046, 2366, 2901, 3228
MaeII ACGT 4 cut(s) 603, 1300, 1696, 2772
MaeIII GTNAC 6 cut(s) 451, 1602, 1842, 1948, 2075, 2455
MalI GATC 8 cut(s) 12, 770, 1290, 1804, 2133, 2444, 2866, 2918
MboI GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
MfeI CAATTG 2 cut(s) 809, 1334
MflI RGATCY 3 cut(s) 1288, 1802, 2864
MhlI GDGCHC 7 cut(s) 390, 928, 1146, 1197, 1229, 2220, 3143
MlsI TGGCCA 4 cut(s) 38, 359, 884, 3212
MluNI TGGCCA 4 cut(s) 38, 359, 884, 3212
Mly113I GGCGCC 1 cut(s) 624
MlyI GAGTC 5 cut(s) 70, 221, 257, 488, 2282
MmeI TCCRAC 9 cut(s) 603, 687, 975, 1309, 1708, 2168, 2886, 3102, 3283
Mox20I TGGCCA 4 cut(s) 38, 359, 884, 3212
Mph1103I ATGCAT 2 cut(s) 2374, 2432
MroNI GCCGGC 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 1280
MscI TGGCCA 4 cut(s) 38, 359, 884, 3212
MslI CAYNNNNRTG 4 cut(s) 980, 1299, 2006, 2739
Msp20I TGGCCA 4 cut(s) 38, 359, 884, 3212
MspA1I CMGCKG 2 cut(s) 34, 2198
MspCI CTTAAG 1 cut(s) 1937
MspI CCGG 8 cut(s) 53, 158, 241, 422, 588, 780, 930, 2213
MunI CAATTG 2 cut(s) 809, 1334
Mva1269I GAATGC 1 cut(s) 2361
MvaI CCWGG 9 cut(s) 476, 680, 749, 916, 1505, 1562, 2576, 3040, 3295
MvnI CGCG 1 cut(s) 258
NaeI GCCGGC 1 cut(s) 589
NarI GGCGCC 1 cut(s) 624
NciI CCSGG 5 cut(s) 53, 158, 781, 930, 931
NcoI CCATGG 1 cut(s) 354
NdeI CATATG 1 cut(s) 2959
NdeII GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
NgoMIV GCCGGC 1 cut(s) 587
NmeAIII GCCGAG 4 cut(s) 148, 600, 816, 1243
NmuCI GTSAC 2 cut(s) 451, 1602
NruI TCGCGA 1 cut(s) 258
NsbI TGCGCA 1 cut(s) 501
NsiI ATGCAT 2 cut(s) 2374, 2432
NspI RCATGY 2 cut(s) 991, 2083
NspV TTCGAA 1 cut(s) 69
OliI CACNNNNGTG 1 cut(s) 1299
PaeR7I CTCGAG 1 cut(s) 860
PceI AGGCCT 1 cut(s) 719
PciI ACATGT 1 cut(s) 987
PciSI GCTCTTC 1 cut(s) 2556
PcsI WCGNNNNNNNCGW 1 cut(s) 1693
PctI GAATGC 1 cut(s) 2361
PdiI GCCGGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 1280
PfeI GAWTC 4 cut(s) 341, 609, 1598, 1967
PfoI TCCNGGA 3 cut(s) 474, 678, 1560
PleI GAGTC 5 cut(s) 69, 220, 257, 487, 2281
PluTI GGCGCC 1 cut(s) 627
PmaCI CACGTG 1 cut(s) 1301
PmlI CACGTG 1 cut(s) 1301
PpsI GAGTC 5 cut(s) 69, 220, 257, 487, 2281
Ppu21I YACGTR 2 cut(s) 1301, 1697
PpuMI RGGWCCY 1 cut(s) 682
PscI ACATGT 1 cut(s) 987
PshAI GACNNNNGTC 3 cut(s) 60, 211, 1336
PshBI ATTAAT 2 cut(s) 1320, 3240
Psp124BI GAGCTC 4 cut(s) 390, 928, 1146, 2220
Psp5II RGGWCCY 1 cut(s) 682
Psp6I CCWGG 9 cut(s) 474, 678, 747, 914, 1503, 1560, 2574, 3038, 3293
PspCI CACGTG 1 cut(s) 1301
PspFI CCCAGC 1 cut(s) 3312
PspGI CCWGG 9 cut(s) 474, 678, 747, 914, 1503, 1560, 2574, 3038, 3293
PspOMI GGGCCC 1 cut(s) 3139
PspPPI RGGWCCY 1 cut(s) 682
PspXI VCTCGAGB 1 cut(s) 860
PstI CTGCAG 3 cut(s) 1030, 1966, 2689
PstNI CAGNNNCTG 7 cut(s) 34, 574, 649, 685, 1034, 1595, 1976
PsuI RGATCY 3 cut(s) 1288, 1802, 2864
PvuII CAGCTG 2 cut(s) 34, 2198
RruI TCGCGA 1 cut(s) 258
RsaI GTAC 8 cut(s) 871, 1242, 1434, 1458, 1497, 1502, 1533, 1895
RsaNI GTAC 8 cut(s) 870, 1241, 1433, 1457, 1496, 1501, 1532, 1894
RseI CAYNNNNRTG 4 cut(s) 980, 1299, 2006, 2739
SacI GAGCTC 4 cut(s) 390, 928, 1146, 2220
SalI GTCGAC 2 cut(s) 199, 301
SapI GCTCTTC 1 cut(s) 2556
Sau3AI GATC 8 cut(s) 10, 768, 1288, 1802, 2131, 2442, 2864, 2916
ScaI AGTACT 1 cut(s) 1458
SchI GAGTC 5 cut(s) 70, 221, 257, 488, 2282
SduI GDGCHC 7 cut(s) 390, 928, 1146, 1197, 1229, 2220, 3143
SexAI ACCWGGT 2 cut(s) 2574, 3293
SfcI CTRYAG 4 cut(s) 1026, 1962, 2067, 2685
SfoI GGCGCC 1 cut(s) 625
Sfr274I CTCGAG 1 cut(s) 860
SfuI TTCGAA 1 cut(s) 69
SinI GGWCC 6 cut(s) 55, 181, 682, 783, 997, 1558
SlaI CTCGAG 1 cut(s) 860
SmaI CCCGGG 1 cut(s) 931
SmiMI CAYNNNNRTG 4 cut(s) 980, 1299, 2006, 2739
SmlI CTYRAG 5 cut(s) 308, 860, 1001, 1937, 2259
SmoI CTYRAG 5 cut(s) 308, 860, 1001, 1937, 2259
SpeI ACTAGT 2 cut(s) 2045, 3227
SseBI AGGCCT 1 cut(s) 719
SspDI GGCGCC 1 cut(s) 623
SspI AATATT 1 cut(s) 2759
SspMI CTAG 7 cut(s) 530, 1059, 1857, 2046, 2366, 2901, 3228
SstI GAGCTC 4 cut(s) 390, 928, 1146, 2220
StuI AGGCCT 1 cut(s) 719
StyI CCWWGG 4 cut(s) 354, 1873, 2499, 3081
TaaI ACNGT 5 cut(s) 114, 379, 1500, 2017, 3005
TaiI ACGT 4 cut(s) 606, 1303, 1699, 2775
TaqI TCGA 7 cut(s) 69, 200, 302, 771, 861, 1251, 3232
TaqII GACCGA 1 cut(s) 800
TatI WGTACW 3 cut(s) 1432, 1456, 1531
TauI GCSGC 4 cut(s) 8, 247, 587, 1116
TfiI GAWTC 4 cut(s) 341, 609, 1598, 1967
TscAI CASTG 6 cut(s) 452, 575, 650, 1030, 1474, 2319
TseFI GTSAC 2 cut(s) 451, 1602
Tsp45I GTSAC 2 cut(s) 451, 1602
TspGWI ACGGA 5 cut(s) 217, 728, 1140, 1358, 1555
TspMI CCCGGG 1 cut(s) 929
TspRI CASTG 6 cut(s) 452, 575, 650, 1030, 1474, 2319
Vha464I CTTAAG 1 cut(s) 1937
VneI GTGCAC 1 cut(s) 1193
VpaK11BI GGWCC 6 cut(s) 55, 181, 682, 783, 997, 1558
VspI ATTAAT 2 cut(s) 1320, 3240
XagI CCTNNNNNAGG 4 cut(s) 664, 1253, 1877, 3060
XapI RAATTY 3 cut(s) 1276, 2165, 2349
XbaI TCTAGA 1 cut(s) 2365
XceI RCATGY 2 cut(s) 991, 2083
XcmI CCANNNNNNNNNTGG 2 cut(s) 352, 1305
XhoI CTCGAG 1 cut(s) 860
XmaI CCCGGG 1 cut(s) 929
XmiI GTMKAC 2 cut(s) 200, 302
XmnI GAANNNNTTC 1 cut(s) 1280
XspI CTAG 7 cut(s) 530, 1059, 1857, 2046, 2366, 2901, 3228
ZraI GACGTC 1 cut(s) 604
ZrmI AGTACT 1 cut(s) 1458
Zsp2I ATGCAT 2 cut(s) 2374, 2432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.