Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000344

Physical Location & Seq

Genomic Coordinates Reverse (-)

470572 .. 478009

Gene Structure 7438 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1005300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2367 bp

ATGCTTCTGGATATTGAGGACGAACCGGCATGGCATGCGGCAGAGAGTGAAGATGAGGATGCGGGTGAGACTGGTAATTACAGTGTTGGGCAGGAGTGTTTGGATAGGCTTGCCATATCCTTGGGTGGGAATACCATTGTTCCCGTGGCGTCTGAGCAATTGCCTGCATATTTGGCTGCCCCTGAGTGGCAGAAGCACCATGCTGCATTGATTGCCCTGGCTCAGATTGCTGAGGGTTGTGCCAAGGTAATGATTAAAAATTTGGAGCAAGTGGTAGCAATGGTCTTGAACTCATTTCAAGATCCACATCCTCGTGTAAGGTGGGCAGCGATAAATGCAATTGGGCAGTTGTCTACTGATTTGGGCCCAGATCTGCAAGTTCAATATCACCAACGGGTGCTCCCGGCATTGGCTGGAGCTATGGATGATTTTCAGAACCCTCGTGTTCAGGCACATGCTGCTTCAGCAGTGCTGAATTTCAGTGAGAATTGCACACCGGATATTTTAACTCCATATTTGGATGGAGTAGTGAGCAAATTGCTTGTACTTCTGCAGAATGGAAAACAAATGGTACAAGAGGGTGCCTTGACTGCTTTGGCATCCGTTGCTGATTCATCTCAGGAGCATTTTCAAAAATACTATGATGCAGTTATGCCTTACCTGAAAGCTATCTTGGTGAATGCCACTGATAAGTCTAATCGCATGCTTCGTGCCAAATCGATGGAGTGTATTAGTTTGGTTGGAATGGCAGTTGGAAAAGAGAAATTCAGGGAAGATGCTAAGCAGGTCATGGAAGTGCTGATGGCTTTGCAAGGATCTCACATGGAGACAGATGATCCAACTACAAGTTACATGTTACAAGCCTGGGCTAGACTCTGCAAGTGCTTAGGACAAGATTTCCTCCCATACATGAGTGTTGTAATGCCTCCTTTGCTTCAATCTGCTCAACTTAAGCCAGATGTAACCATCACATCTGCAGATGACGAAAATGATATTGATGACTCTGATGATGATAGTATGGAAACAATTACTCTTGGGGATAAAAGGATTGGAATCAAGACTAGTGTCTTGGAGGAGAAAGCTACAGCTTGCAATATGCTGTGTTGCTATGCTGATGAGCTGAAAGAAGGATTCTTTCCCTGGATTGATCAGGTTGCCCCAACTTTGGTTCCACTTCTTAAATTTTATTTCCATGAAGAAGTTAGGAAGGCAGCTGTTTCAGCAATGCCGGAGCTACTGCGTTCTGCAAAGTTGGCAATAGAGAAAGGCCAAGCTCAAGGTCGTAATGAAACATATATAAAGCAATTGTCTGACTACATTGTTCCAGCTCTGGTGGAAGCATTACATAAGGAACCTGATACTGAAATCTGTGCGAATATATTGGATGCATTAAATGAATGCTTACAGACATCTGGACCACTTTTAGATGAAGGCCAGGTGAGATCCATCGTGGAGGAGATAAAGCAGGTGATCACTGCCAGTTCTAGTAGAAAAAGAGAGAGAGCAGAAAGGACCAAAGCAGAAGACTTTGATGCTGAGGAACAGGAGCTAATTAAAGAGGAAAATGAACAAGAGGAAGAAGTTTTTGATCAAGTGGGAGAAATCATTGGAACTTTGATTAAAACGTTTAAAGCCTCTTTCTTGCCTTTCTTTGATGAGCTATCATCGTATTTAACACCTATGTGGGCCAAGGATAAAACACCTGAAGAGAGAAGGATTGCAATATGCATCTTTGATGAAGTAGCAGAGCAGTGTCGTGAAGCAGCTGTAAAATATTATGACACATTTCTTCCTTTTCTGTTGGAGGCTTGCAATGACGAAAACCCTGATGTTCGACAGGCAGCTGTATATGGACTTGGTATTTGTTCAGAGTTTGGTGGAGCAATAATTAAACCTCTTGTTGGCGAGGCTCTATCAAGGCTGAACGCTGTGATTCAGCATCCTAATGCCCAACAATCTGAAAATGTAATGGCATATGATAATGCTGTTTCTGCTTTAGGAAAAATTAGCCAATTCCACCGTGACAGTATTGATGCAGCTCAGGTCATTCCTGCATGGTTGAACTGTTTACCGATTAAAGGAGACTTGGTTGAAGCCAAAGTTGTTCATGACCAACTTTGTTCTATGGTAGAGAGGTCTGATCGGGAACTTTTGGGTCCCAACAATCAGTATCTTCCCAAAATTGTTGCAGTGTTTGCAGAGGTTCTATGTGCTGGTAAGGATCTGGCAACTGAACAGACTGCAAACCGAATGATTAAATTGCTAAGGCAGCTTCAGCAGACTTTGCCACCAGCTACATTGGCTTCAACGTGGTCGTCCTTGCAACCCCAGCAACAGCTTGCTCTGCAATCCATCCTCTCCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

789

Amino Acids

87.17

Weight (kDa)

4.64

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Importin_rep_4	PF18808	1 - 48	6.1e-08	Importin repeat
HEAT_2	PF13646	57 - 156	5.6e-07	HEAT repeats
HEAT_EZ	PF13513	63 - 117	2.7e-08	HEAT-like repeat
TOR1L1_N	PF24714	69 - 265	1.7e-08	TORTIFOLIA1 N-terminal
HEAT_EZ	PF13513	147 - 201	8.6e-07	HEAT-like repeat
TPR_IMB1	PF25574	150 - 319	1.4e-11	Importin subunit beta-1-like, TPR repeats
Importin_rep_6	PF18829	457 - 560	1.4e-19	Importin repeat 6
HEAT_EZ	PF13513	568 - 620	4.2e-08	HEAT-like repeat
HEAT	PF02985	596 - 623	4.3e-06	HEAT repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19820
fragaria_vesca	FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica	MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica	Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis	pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis	RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata	RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora	Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii	Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa	Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha	Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana	Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1456
Acc36I	ACCTGC	2 cut(s)	775, 1456
AccB1I	GGYRCC	1 cut(s)	581
AccI	GTMKAC	1 cut(s)	353
AciI	CCGC	2 cut(s)	38, 62
AclI	AACGTT	1 cut(s)	1625
AclWI	GGATC	5 cut(s)	296, 823, 830, 1437, 2229
AcsI	RAATTY	4 cut(s)	259, 475, 764, 1181
AcuI	CTGAAG	3 cut(s)	447, 1725, 2258
AcyI	GRCGYC	1 cut(s)	149
AfaI	GTAC	2 cut(s)	546, 573
AfiI	CCNNNNNNNGG	6 cut(s)	126, 186, 409, 1165, 1901, 2078
AflII	CTTAAG	1 cut(s)	950
AflIII	ACRYGT	1 cut(s)	852
AgsI	TTSAA	8 cut(s)	289, 299, 383, 632, 938, 2062, 2093, 2307
AhlI	ACTAGT	1 cut(s)	1061
AjnI	CCWGG	4 cut(s)	216, 863, 1139, 1434
AjuI	GAANNNNNNNTTGG	2 cut(s)	656, 688
AleI	CACNNNNGTG	1 cut(s)	1681
Alw21I	GWGCWC	1 cut(s)	402
Alw26I	GTCTC	3 cut(s)	62, 821, 2076
AlwI	GGATC	5 cut(s)	296, 823, 830, 1437, 2229
AoxI	GGCC	4 cut(s)	364, 1267, 1432, 1686
ApaI	GGGCCC	1 cut(s)	368
ApeKI	GCWGC	9 cut(s)	176, 203, 326, 458, 1211, 1763, 1841, 2036, 2269
ApoI	RAATTY	4 cut(s)	259, 475, 764, 1181
ArsI	GACNNNNNNTTYG	2 cut(s)	1845, 1877
AspS9I	GGNCC	6 cut(s)	364, 365, 1415, 1512, 1686, 2156
AsuC2I	CCSGG	1 cut(s)	404
AsuHPI	GGTGA	5 cut(s)	77, 380, 688, 1450, 1480
AvaII	GGWCC	3 cut(s)	1415, 1512, 2156
BaeGI	GKGCMC	1 cut(s)	368
BanI	GGYRCC	1 cut(s)	581
BanII	GRGCYC	1 cut(s)	368
BauI	CACGAG	2 cut(s)	312, 441
BbsI	GAAGAC	1 cut(s)	1530
Bbv12I	GWGCWC	1 cut(s)	402
BbvCI	CCTCAGC	2 cut(s)	231, 1536
BbvI	GCAGC	9 cut(s)	163, 190, 338, 445, 1223, 1775, 1853, 2048, 2281
BccI	CCATC	6 cut(s)	515, 715, 796, 974, 1454, 2360
BciT130I	CCWGG	4 cut(s)	218, 865, 1141, 1436
BclI	TGATCA	3 cut(s)	1147, 1470, 1588
BcnI	CCSGG	1 cut(s)	404
BcoDI	GTCTC	3 cut(s)	62, 821, 2076
BcuI	ACTAGT	1 cut(s)	1061
BfaI	CTAG	3 cut(s)	870, 1062, 1485
BfmI	CTRYAG	3 cut(s)	551, 975, 1083
BfrI	CTTAAG	1 cut(s)	950
BfuAI	ACCTGC	2 cut(s)	775, 1456
BglII	AGATCT	1 cut(s)	370
BlpI	GCTNAGC	1 cut(s)	780
Bme1390I	CCNGG	5 cut(s)	218, 404, 865, 1141, 1436
Bme18I	GGWCC	3 cut(s)	1415, 1512, 2156
BmgT120I	GGNCC	6 cut(s)	364, 365, 1415, 1512, 1686, 2156
BmiI	GGNNCC	6 cut(s)	366, 583, 1170, 1353, 2157, 2158
BmrFI	CCNGG	5 cut(s)	218, 404, 865, 1141, 1436
BmsI	GCATC	9 cut(s)	49, 608, 634, 766, 1375, 1522, 1737, 1948, 2023
BoxI	GACNNNNGTC	1 cut(s)	1064
BpiI	GAAGAC	1 cut(s)	1530
BpmI	CTGGAG	1 cut(s)	435
Bpu10I	CCTNAGC	5 cut(s)	231, 886, 1536, 2040, 2264
Bpu1102I	GCTNAGC	1 cut(s)	780
BpuEI	CTTGAG	1 cut(s)	1260
BpuMI	CCSGG	1 cut(s)	404
Bsa29I	ATCGAT	1 cut(s)	719
BsaBI	GATNNNNATC	2 cut(s)	306, 1052
BsaHI	GRCGYC	1 cut(s)	149
BsaJI	CCNNGG	7 cut(s)	120, 144, 216, 243, 864, 1139, 1689
BsaWI	WCCGGW	1 cut(s)	496
BsaXI	ACNNNNNCTCC	4 cut(s)	384, 414, 2073, 2103
Bsc4I	CCNNNNNNNGG	6 cut(s)	126, 186, 409, 1165, 1901, 2078
Bse118I	RCCGGY	1 cut(s)	25
Bse1I	ACTGG	2 cut(s)	76, 1479
Bse3DI	GCAATG	3 cut(s)	285, 1230, 1819
Bse8I	GATNNNNATC	2 cut(s)	306, 1052
BseBI	CCWGG	4 cut(s)	218, 865, 1141, 1436
BseCI	ATCGAT	1 cut(s)	719
BseDI	CCNNGG	7 cut(s)	120, 144, 216, 243, 864, 1139, 1689
BseGI	GGATG	8 cut(s)	64, 307, 430, 526, 599, 1390, 1939, 2352
BseJI	GATNNNNATC	2 cut(s)	306, 1052
BseLI	CCNNNNNNNGG	6 cut(s)	126, 186, 409, 1165, 1901, 2078
BseMI	GCAATG	3 cut(s)	285, 1230, 1819
BseMII	CTCAG	7 cut(s)	144, 174, 222, 236, 632, 1527, 2054
BseNI	ACTGG	2 cut(s)	76, 1479
BseRI	GAGGAG	3 cut(s)	1088, 1469, 2350
BseSI	GKGCMC	1 cut(s)	368
BseXI	GCAGC	9 cut(s)	163, 190, 338, 445, 1223, 1775, 1853, 2048, 2281
BseYI	CCCAGC	1 cut(s)	2328
BshFI	GGCC	4 cut(s)	366, 1269, 1434, 1688
BshNI	GGYRCC	1 cut(s)	581
BshVI	ATCGAT	1 cut(s)	719
BsiHKAI	GWGCWC	1 cut(s)	402
BsiSI	CCGG	4 cut(s)	26, 404, 497, 1229
BslFI	GGGAC	1 cut(s)	2142
BslI	CCNNNNNNNGG	6 cut(s)	126, 186, 409, 1165, 1901, 2078
BsmAI	GTCTC	3 cut(s)	62, 821, 2076
BsmFI	GGGAC	1 cut(s)	2142
BsmI	GAATGC	2 cut(s)	685, 1403
BsnI	GGCC	4 cut(s)	366, 1269, 1434, 1688
Bsp120I	GGGCCC	1 cut(s)	364
Bsp1286I	GDGCHC	2 cut(s)	368, 402
Bsp1720I	GCTNAGC	1 cut(s)	780
BspACI	CCGC	2 cut(s)	38, 62
BspANI	GGCC	4 cut(s)	366, 1269, 1434, 1688
BspCNI	CTCAG	7 cut(s)	145, 175, 223, 235, 631, 1528, 2053
BspDI	ATCGAT	1 cut(s)	719
BspHI	TCATGA	1 cut(s)	2107
BspLI	GGNNCC	6 cut(s)	366, 583, 1170, 1353, 2157, 2158
BspMAI	CTGCAG	2 cut(s)	555, 979
BspMI	ACCTGC	2 cut(s)	775, 1456
BspPI	GGATC	5 cut(s)	296, 823, 830, 1437, 2229
BspT107I	GGYRCC	1 cut(s)	581
BspTI	CTTAAG	1 cut(s)	950
BsrDI	GCAATG	3 cut(s)	285, 1230, 1819
BsrFI	RCCGGY	1 cut(s)	25
BsrI	ACTGG	2 cut(s)	76, 1479
BssAI	RCCGGY	1 cut(s)	25
BssECI	CCNNGG	7 cut(s)	120, 144, 216, 243, 864, 1139, 1689
BssNI	GRCGYC	1 cut(s)	149
BssSI	CACGAG	2 cut(s)	312, 441
BssT1I	CCWWGG	3 cut(s)	120, 243, 1689
Bst2BI	CACGAG	2 cut(s)	312, 441
Bst2UI	CCWGG	4 cut(s)	218, 865, 1141, 1436
Bst4CI	ACNGT	4 cut(s)	83, 2021, 2027, 2066
Bst6I	CTCTTC	1 cut(s)	1701
BstACI	GRCGYC	1 cut(s)	149
BstAFI	CTTAAG	1 cut(s)	950
BstAPI	GCANNNNNTGC	6 cut(s)	35, 212, 458, 605, 2195, 2284
BstC8I	GCNNGC	7 cut(s)	36, 111, 165, 704, 1090, 1810, 2340
BstDSI	CCRYGG	1 cut(s)	144
BstF5I	GGATG	8 cut(s)	64, 307, 430, 526, 599, 1390, 1939, 2352
BstMAI	GTCTC	3 cut(s)	62, 821, 2076
BstNI	CCWGG	4 cut(s)	218, 865, 1141, 1436
BstNSI	RCATGY	4 cut(s)	38, 458, 706, 856
BstPAI	GACNNNNGTC	1 cut(s)	1064
BstSCI	CCNGG	5 cut(s)	216, 402, 863, 1139, 1434
BstSFI	CTRYAG	3 cut(s)	551, 975, 1083
BstSLI	GKGCMC	1 cut(s)	368
BstV1I	GCAGC	9 cut(s)	163, 190, 338, 445, 1223, 1775, 1853, 2048, 2281
BstV2I	GAAGAC	1 cut(s)	1530
BstX2I	RGATCY	5 cut(s)	301, 370, 815, 1442, 2221
BstXI	CCANNNNNNTGG	2 cut(s)	121, 721
BstYI	RGATCY	5 cut(s)	301, 370, 815, 1442, 2221
Bsu15I	ATCGAT	1 cut(s)	719
BsuRI	GGCC	4 cut(s)	366, 1269, 1434, 1688
BsuTUI	ATCGAT	1 cut(s)	719
BtgI	CCRYGG	1 cut(s)	144
BtsCI	GGATG	8 cut(s)	64, 307, 430, 526, 599, 1390, 1939, 2352
BtsI	GCAGTG	4 cut(s)	474, 1473, 1757, 2196
BtsIMutI	CAGTG	7 cut(s)	88, 474, 487, 684, 1473, 1757, 2196
BveI	ACCTGC	2 cut(s)	775, 1456
Cac8I	GCNNGC	7 cut(s)	36, 111, 165, 704, 1090, 1810, 2340
CciI	TCATGA	1 cut(s)	2107
Cfr10I	RCCGGY	1 cut(s)	25
Cfr13I	GGNCC	6 cut(s)	364, 365, 1415, 1512, 1686, 2156
ClaI	ATCGAT	1 cut(s)	719
CseI	GACGC	1 cut(s)	138
Csp6I	GTAC	2 cut(s)	545, 572
CviQI	GTAC	2 cut(s)	545, 572
DraI	TTTAAA	1 cut(s)	1630
Eam1104I	CTCTTC	1 cut(s)	1701
EarI	CTCTTC	1 cut(s)	1701
Eco130I	CCWWGG	3 cut(s)	120, 243, 1689
Eco24I	GRGCYC	1 cut(s)	368
Eco47I	GGWCC	3 cut(s)	1415, 1512, 2156
Eco57I	CTGAAG	3 cut(s)	447, 1725, 2258
EcoO109I	RGGNCCY	1 cut(s)	2156
EcoRII	CCWGG	4 cut(s)	216, 863, 1139, 1434
EcoT14I	CCWWGG	3 cut(s)	120, 243, 1689
EcoT22I	ATGCAT	2 cut(s)	1390, 1730
EcoT38I	GRGCYC	1 cut(s)	368
ErhI	CCWWGG	3 cut(s)	120, 243, 1689
FaqI	GGGAC	1 cut(s)	2142
FauI	CCCGC	1 cut(s)	55
FauNDI	CATATG	1 cut(s)	1975
FbaI	TGATCA	3 cut(s)	1147, 1470, 1588
FblI	GTMKAC	1 cut(s)	353
FokI	GGATG	8 cut(s)	71, 294, 437, 533, 586, 1397, 1926, 2339
FriOI	GRGCYC	1 cut(s)	368
FspBI	CTAG	3 cut(s)	870, 1062, 1485
GsaI	CCCAGC	1 cut(s)	2332
GsuI	CTGGAG	1 cut(s)	435
HaeIII	GGCC	4 cut(s)	366, 1269, 1434, 1688
HapII	CCGG	4 cut(s)	26, 404, 497, 1229
HgaI	GACGC	1 cut(s)	138
Hin1I	GRCGYC	1 cut(s)	149
HinfI	GANTC	6 cut(s)	611, 873, 1001, 1053, 1131, 1933
HpaII	CCGG	4 cut(s)	26, 404, 497, 1229
HphI	GGTGA	5 cut(s)	77, 380, 688, 1450, 1480
Hpy166II	GTNNAC	2 cut(s)	354, 2069
Hpy188I	TCNGA	8 cut(s)	154, 225, 435, 1006, 1312, 1870, 1960, 2140
Hpy188III	TCNNGA	9 cut(s)	8, 286, 299, 620, 1057, 1413, 1757, 2108, 2144
Hpy8I	GTNNAC	2 cut(s)	354, 2069
HpyAV	CCTTC	4 cut(s)	1121, 1201, 1424, 1707
HpyCH4III	ACNGT	4 cut(s)	83, 2021, 2027, 2066
HpyCH4IV	ACGT	2 cut(s)	1625, 2309
HpySE526I	ACGT	2 cut(s)	1625, 2309
Hsp92I	GRCGYC	1 cut(s)	149
KflI	GGGWCCC	1 cut(s)	2156
Ksp22I	TGATCA	3 cut(s)	1147, 1470, 1588
LmnI	GCTCC	7 cut(s)	265, 405, 416, 622, 1231, 1546, 1880
Lsp1109I	GCAGC	9 cut(s)	163, 190, 338, 445, 1223, 1775, 1853, 2048, 2281
LweI	GCATC	9 cut(s)	49, 608, 634, 766, 1375, 1522, 1737, 1948, 2023
MaeI	CTAG	3 cut(s)	870, 1062, 1485
MaeII	ACGT	2 cut(s)	1625, 2309
MaeIII	GTNAC	4 cut(s)	848, 855, 961, 2021
MboII	GAAGA	8 cut(s)	62, 785, 1208, 1535, 1589, 1718, 1781, 2165
MfeI	CAATTG	3 cut(s)	158, 339, 1304
MflI	RGATCY	5 cut(s)	301, 370, 815, 1442, 2221
MhlI	GDGCHC	2 cut(s)	368, 402
MlyI	GAGTC	2 cut(s)	867, 995
MmeI	TCCRAC	4 cut(s)	721, 733, 863, 1782
Mph1103I	ATGCAT	2 cut(s)	1390, 1730
MslI	CAYNNNNRTG	4 cut(s)	312, 794, 1681, 1944
MspA1I	CMGCKG	3 cut(s)	1214, 1766, 1844
MspCI	CTTAAG	1 cut(s)	950
MspI	CCGG	4 cut(s)	26, 404, 497, 1229
MspR9I	CCNGG	5 cut(s)	218, 404, 865, 1141, 1436
MunI	CAATTG	3 cut(s)	158, 339, 1304
Mva1269I	GAATGC	2 cut(s)	685, 1403
MvaI	CCWGG	4 cut(s)	218, 865, 1141, 1436
NciI	CCSGG	1 cut(s)	404
NdeI	CATATG	1 cut(s)	1975
NlaIV	GGNNCC	6 cut(s)	366, 583, 1170, 1353, 2157, 2158
NmuCI	GTSAC	1 cut(s)	2021
NsiI	ATGCAT	2 cut(s)	1390, 1730
NspI	RCATGY	4 cut(s)	38, 458, 706, 856
OliI	CACNNNNGTG	1 cut(s)	1681
PaeI	GCATGC	2 cut(s)	38, 706
PagI	TCATGA	1 cut(s)	2107
PaqCI	CACCTGC	1 cut(s)	1456
PciI	ACATGT	1 cut(s)	852
PcsI	WCGNNNNNNNCGW	1 cut(s)	706
PctI	GAATGC	2 cut(s)	685, 1403
PfeI	GAWTC	4 cut(s)	611, 1053, 1131, 1933
PleI	GAGTC	2 cut(s)	867, 995
PpsI	GAGTC	2 cut(s)	867, 995
PpuMI	RGGWCCY	1 cut(s)	2156
PscI	ACATGT	1 cut(s)	852
PshAI	GACNNNNGTC	1 cut(s)	1064
Psp1406I	AACGTT	1 cut(s)	1625
Psp5II	RGGWCCY	1 cut(s)	2156
Psp6I	CCWGG	4 cut(s)	216, 863, 1139, 1434
PspFI	CCCAGC	1 cut(s)	2328
PspGI	CCWGG	4 cut(s)	216, 863, 1139, 1434
PspN4I	GGNNCC	6 cut(s)	366, 583, 1170, 1353, 2157, 2158
PspOMI	GGGCCC	1 cut(s)	364
PspPI	GGNCC	6 cut(s)	364, 365, 1415, 1512, 1686, 2156
PspPPI	RGGWCCY	1 cut(s)	2156
PstI	CTGCAG	2 cut(s)	555, 979
PsuI	RGATCY	5 cut(s)	301, 370, 815, 1442, 2221
PvuII	CAGCTG	3 cut(s)	1214, 1766, 1844
RsaI	GTAC	2 cut(s)	546, 573
RsaNI	GTAC	2 cut(s)	545, 572
RseI	CAYNNNNRTG	4 cut(s)	312, 794, 1681, 1944
Sau96I	GGNCC	6 cut(s)	364, 365, 1415, 1512, 1686, 2156
SchI	GAGTC	2 cut(s)	867, 995
ScrFI	CCNGG	5 cut(s)	218, 404, 865, 1141, 1436
SduI	GDGCHC	2 cut(s)	368, 402
SfaNI	GCATC	9 cut(s)	49, 608, 634, 766, 1375, 1522, 1737, 1948, 2023
SfcI	CTRYAG	3 cut(s)	551, 975, 1083
SinI	GGWCC	3 cut(s)	1415, 1512, 2156
SmiMI	CAYNNNNRTG	4 cut(s)	312, 794, 1681, 1944
SmlI	CTYRAG	2 cut(s)	950, 1275
SmoI	CTYRAG	2 cut(s)	950, 1275
SpeI	ACTAGT	1 cut(s)	1061
SphI	GCATGC	2 cut(s)	38, 706
SsiI	CCGC	2 cut(s)	38, 62
SspI	AATATT	1 cut(s)	1775
SspMI	CTAG	3 cut(s)	870, 1062, 1485
StyD4I	CCNGG	5 cut(s)	216, 402, 863, 1139, 1434
StyI	CCWWGG	3 cut(s)	120, 243, 1689
TaaI	ACNGT	4 cut(s)	83, 2021, 2027, 2066
TaiI	ACGT	2 cut(s)	1628, 2312
TaqI	TCGA	2 cut(s)	719, 1834
TatI	WGTACW	1 cut(s)	544
TauI	GCSGC	1 cut(s)	41
TfiI	GAWTC	4 cut(s)	611, 1053, 1131, 1933
TscAI	CASTG	7 cut(s)	88, 474, 487, 691, 1480, 1757, 2196
TseFI	GTSAC	1 cut(s)	2021
TseI	GCWGC	9 cut(s)	176, 203, 326, 458, 1211, 1763, 1841, 2036, 2269
Tsp45I	GTSAC	1 cut(s)	2021
TspDTI	ATGAA	8 cut(s)	603, 1209, 1302, 1410, 1443, 1581, 1752, 2096
TspGWI	ACGGA	1 cut(s)	592
TspRI	CASTG	7 cut(s)	88, 474, 487, 691, 1480, 1757, 2196
Vha464I	CTTAAG	1 cut(s)	950
VpaK11BI	GGWCC	3 cut(s)	1415, 1512, 2156
XapI	RAATTY	4 cut(s)	259, 475, 764, 1181
XceI	RCATGY	4 cut(s)	38, 458, 706, 856
XcmI	CCANNNNNNNNNTGG	1 cut(s)	142
XmiI	GTMKAC	1 cut(s)	353
XspI	CTAG	3 cut(s)	870, 1062, 1485
Zsp2I	ATGCAT	2 cut(s)	1390, 1730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.