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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 84, 151 |
| AgsI | TTSAA | 1 cut(s) | 161 |
| ApoI | RAATTY | 2 cut(s) | 84, 151 |
| AsuHPI | GGTGA | 1 cut(s) | 37 |
| BpuEI | CTTGAG | 1 cut(s) | 122 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 14 |
| CviAII | CATG | 2 cut(s) | 109, 116 |
| FaeI | CATG | 2 cut(s) | 112, 119 |
| FaiI | YATR | 4 cut(s) | 90, 110, 117, 128 |
| FatI | CATG | 2 cut(s) | 108, 115 |
| FspEI | CC | 9 cut(s) | 8, 20, 36, 47, 51, 54, 121, 125, 161 |
| Hin1II | CATG | 2 cut(s) | 112, 119 |
| HphI | GGTGA | 1 cut(s) | 37 |
| Hsp92II | CATG | 2 cut(s) | 112, 119 |
| LpnPI | CCDG | 1 cut(s) | 20 |
| MluCI | AATT | 3 cut(s) | 84, 93, 151 |
| MnlI | CCTC | 1 cut(s) | 48 |
| NlaIII | CATG | 2 cut(s) | 112, 119 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 57, 89 |
| SetI | ASST | 1 cut(s) | 144 |
| SgeI | CNNG | 6 cut(s) | 19, 73, 80, 121, 128, 151 |
| SmlI | CTYRAG | 1 cut(s) | 137 |
| SmoI | CTYRAG | 1 cut(s) | 137 |
| Sse9I | AATT | 3 cut(s) | 84, 93, 151 |
| TasI | AATT | 3 cut(s) | 84, 93, 151 |
| TspDTI | ATGAA | 2 cut(s) | 17, 104 |
| XapI | RAATTY | 2 cut(s) | 84, 151 |