Rh3BG368400

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000344
Physical Location & Seq
Forward (+)
45953543 .. 45963657
10115 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG368400.1

Sequence Viewer

Length: 3120 bp
ATGGCCGCCGATCCAAGTCAGCAGCAGCAGCTGGCCATAATTCTTGGCCCGGACCCGAGTCAGTTCGAAACCCTAATCTCTCATCTCATGTCCTCCAGCAACGAGCAACGGTCGCAGGCCGAGGCCCTCTTCAACCTCTGCAAGCAGGCCCACCCGGACGCCCTCTTACTGAAGCTGGTCCATGTGTTGCAGGCGTCGACCCGACCCGAGGCCCGCACCATGTCCGTCATCCTCCTCCGGCGGCAGCTCACTCGCGACGACTCTTTCCTCTGGCCCCGCCTCAGCCCCTCCACTCAGTCGACCCTCAAGTCCACACTCCTCACTTGCCTCCAATCAGAATCCACCAAATCCATGGCCAAGAAGCTCTGCGACACCGTTTCGGAGCTCGCTTCTGCCATTTTACCCGATAACCAATGGCCGGAGCTATTGCCGTTCATGTTCCACTGCGTCACCTCCGATAATTCCAAGCTCCAGGAGTCTGCGCTTTTGATTTTTGCGCAGTTGGCGCATTACATCGGCGAAACCCTAGTCCCGCAGTTGACTACGCTCCACTCAGTCTTCTACCAGTGCCTGTCCAACGCCGCCGGCAAGAGCGCCGACGTCAGAATTGCAGCACTCGGCGCCTCTGTCAATTTCATCCAGTGCCTGACCAGCGCCTCCGACAGGGACAGGTTCCAGGACCTGTTGCCCTTGATGATGCAGACTTTGACGGAGGCCTTGAACGGCGGTCAGGAGGCCACGGCGCAGGAGGCGCTGGAGTTGCTGATCGAGTTGGCCGGGACCGAGCCGAGGTTCCTGCGGAGGCAATTGGTGGATGTGGTTGGCTCTATGCTTCAAATTGCGGAGGCTGCGTCCCTCGAGGAAGGTACAAGGCATTTGGCCATTGAGTTTGTCATCACTCTGGCTGAGGCCAGGGAGAGAGCCCCCGGGATGATGAGGAAGTTGCCTCAGTTTATTCATAGGTTGTTTGCCATACTAATGAACATGTTGTTGGACCTTGAGGACGACCCCGACTGGCACACTGCAGATACTGAGAATGAGGATGCTGGTGAGACTAGCAATTATGGCTTTGGACAGGAGTGTTTGGACAGACTTTCAATTTCATTGGGCGGCAATACCATTGTCCCTGTTGCTTCCGAGCTTTTTCCGTCTTACTTGGTTGCTCCTGAATGGCAAAAGCACCACGCCGTGCACATTGCCCTTGCCCAGATTGCCGAGGGGTGCTCTAAGGTAATGGTACAAAACCTCGAACAGGTGGTATCAATGGTATTGAATTCGTTCCAAGATCCTCACCCACGTGTGAGGTGGGCAGCAATTAATGCAGTTGGGCAATTGTCAACGGATTTAGGCCCAAACTTACAAGAAAAGTATCACCAGCGAGTGCTGCCTGCATTAGCTGGAGCAATGGATGACTTCCAAAATCCACGAGTACAGGCCCATGCCGCTTCAGCAGTACTCAATTTCAGTGAAAACTGCACCCCAGATATTTTGGTACCGTACCTGGATGGAATTGTTAGCAAGCTTCTTGTACTTCTACAGAATGGAAAGCAGATGGTCCAGGAGGGGGCTTTAACAGCTTTAGCATCAGTTGCTGATTCGTCACAGGAGCAATTCCAGAAGTATTATGATGCTGTAATGCCTTACTTGAAAGCTATTTTGGTTAATGCAAATGACAAAGCTAATCGTATGCTACGTGCAAAATCAATGGAGTGTATTAGTTTGGTTGGAATGGCTGTCGGGAAGGATAAGTTCAGGGATGACGCAAAGCAGGTTATGGAAGTCTTGATGTCATTGCAAGGATCTGAAATGGAGGCAGATGACCCCACAACAAGTTATATGTTACAAGCATGGGCTAGACTTTGCAAATGCCTTGGGCAGGATTTTCTCCCGTACATGAATGTTGTCATGCCTTCTTTGCTTCAGTCTGCTCAGCTTAAGCCTGATGTAACAATTACTTCTGCAGATTCAGATGCAGAAATTGATGAGGATGATGATAGCATTGAAATGATTACTGTTGGCGATAAAAGGATAGGGATTAAGACTAGTGTTCTGGAGGAAAAAGCTACAGCCTGTAACATGCTATGTTGCTATGCTGATGAGTTAAAGGAAGGATTTTTCCCGTGGATTGATCAGGTTGCTCCAACATTGGTTCCTCTTCTAAAATTTTATTTCCATGAAGAAGTTAGAAAGGCAGCTGTTTCAGCGATGCCGGAGCTCCTACGCTCAGCCAAATTAGCAGTAGAGAAGGGACAATCTCAAGGTCATAATGAGTCCTACGTAAAGCAGTTATCTGATTACATAATACCAGCACTGGTTGAAGCATTACACAAGGAACCTGAGGTTGAAATTTGTGCCAGCATTCTAGATGCATTGAACGAATGTGTGCAGAGTTCTGGGCCACTTCTTGATGAAAACCAAGTTCGATGCATCGTGGATGAGATCAAACAGGTGGTTACAGCAAGCTCATCCAGAAAACAAGAGCGAGCAGAAAGGGCCAAGGCAGAGGACTTTGATGCAGAAGAAGGGGAACTTCTGAAGGAGGAAAATGAACAAGAAGAAGAGCTTTTTGACCTGGTTGGTGATTGCTTGGGAACTTTGATTAAGACATTCAAGGCATCTTTCTTGCCTTTCTTTGACGAGCTTTCCTCTTACATAACACCTATGTTGGGTAAGGATAAAACTGCAGAAGAGAGGAGAATTGCCATTTGCATATTTGATGATATGGCAGAGCACTGCCGTGAAGCTGCTCTTAAATATTATGATACTTACGTTCCTTTCCTACTGGAAGCCTGCAATGATGAAAGTTCAGATGTTCGTCAGGCTGCTGTTTATGGGGTTGGTCTTTGTGCGGAGTATGGTGGATCTGTGTTCAAACCTCTTGTTGGTGAAGCTCTTTCTAGGTTGGATATGGTGATCAGGAACCCCAATTCACAACACTCTGACAATATGATGGCATATGACAATGCTGTTTCAGCTCTTGGGAAAATATGCCAGTTCCACCGTGATAGTATAGATGCACCACAGCTTGTCCCTGCCTGGTTAGGTTGTCTGCCTATAAAAGGTGATTTGATTGAGGCCAAGGTTGTCCATGACCAGCTATGTTCAATGGTTGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

1039

Amino Acids

115.07

Weight (kDa)

4.75

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF577 PF04510 101 - 253 6e-07 Repeat of unknown function (DUF577)
TPR_IPO5 PF25780 115 - 274 2e-36 IPO5-like, TPR repeats
Importin_rep_4 PF18808 285 - 376 2.1e-22 Importin repeat
HEAT_EZ PF13513 392 - 445 4.1e-06 HEAT-like repeat
TOR1L1_N PF24714 392 - 578 2e-09 TORTIFOLIA1 N-terminal
HEAT_GCN1 PF23271 400 - 556 4.3e-06 Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EZ PF13513 475 - 529 3.5e-07 HEAT-like repeat
TPR_IMB1 PF25574 478 - 648 6.9e-12 Importin subunit beta-1-like, TPR repeats
Importin_rep_6 PF18829 784 - 890 1.7e-18 Importin repeat 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19820
fragaria_vesca FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 307
AatII GACGTC 1 cut(s) 603
AbsI CCTCGAGG 1 cut(s) 857
Acc16I TGCGCA 1 cut(s) 498
Acc36I ACCTGC 1 cut(s) 1759
Acc65I GGTACC 1 cut(s) 1492
AccB1I GGYRCC 2 cut(s) 620, 1492
AccI GTMKAC 2 cut(s) 197, 299
AccII CGCG 1 cut(s) 255
AclWI GGATC 4 cut(s) 5, 1280, 1807, 2869
AcoI YGGCCR 6 cut(s) 3, 33, 354, 416, 774, 879
AcsI RAATTY 3 cut(s) 1273, 2162, 2346
AcuI CTGAAG 4 cut(s) 191, 1431, 1904, 2555
AcvI CACGTG 1 cut(s) 1298
AcyI GRCGYC 4 cut(s) 159, 194, 600, 621
AdeI CACNNNGTG 1 cut(s) 1189
AfaI GTAC 8 cut(s) 868, 1239, 1431, 1455, 1494, 1499, 1530, 1892
AflII CTTAAG 1 cut(s) 1934
AflIII ACRYGT 2 cut(s) 984, 1297
AhlI ACTAGT 1 cut(s) 2042
AjnI CCWGG 7 cut(s) 471, 675, 911, 1500, 1557, 2571, 3035
AjuI GAANNNNNNNTTGG 4 cut(s) 974, 1006, 1640, 1672
AleI CACNNNNGTG 2 cut(s) 1296, 2736
Alw21I GWGCWC 5 cut(s) 387, 1194, 1226, 2217, 2733
Alw26I GTCTC 1 cut(s) 1046
Alw44I GTGCAC 1 cut(s) 1190
AlwI GGATC 4 cut(s) 5, 1280, 1807, 2869
AlwNI CAGNNNCTG 6 cut(s) 31, 571, 646, 682, 1031, 1592
Ama87I CYCGRG 4 cut(s) 55, 206, 857, 926
ApaLI GTGCAC 1 cut(s) 1190
ApoI RAATTY 3 cut(s) 1273, 2162, 2346
ArsI GACNNNNNNTTYG 2 cut(s) 513, 545
AseI ATTAAT 1 cut(s) 1317
Asp700I GAANNNNTTC 1 cut(s) 1277
Asp718I GGTACC 1 cut(s) 1492
AspLEI GCGC 8 cut(s) 484, 499, 508, 596, 623, 656, 745, 754
AsuC2I CCSGG 5 cut(s) 50, 155, 778, 927, 928
AsuHPI GGTGA 8 cut(s) 442, 1061, 1283, 1364, 2591, 2897, 2923, 3074
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 4 cut(s) 55, 206, 857, 926
AvaII GGWCC 6 cut(s) 52, 178, 679, 780, 994, 1555
AxyI CCTNAGG 1 cut(s) 2337
BaeGI GKGCMC 1 cut(s) 1194
BaeI ACNNNNGTAYC 2 cut(s) 2754, 2787
BalI TGGCCA 3 cut(s) 35, 356, 881
BanI GGYRCC 2 cut(s) 620, 1492
BanII GRGCYC 3 cut(s) 387, 925, 2217
BauI CACGAG 1 cut(s) 1425
BbrPI CACGTG 1 cut(s) 1298
BbsI GAAGAC 1 cut(s) 550
Bbv12I GWGCWC 5 cut(s) 387, 1194, 1226, 2217, 2733
BbvCI CCTCAGC 2 cut(s) 281, 906
BccI CCATC 3 cut(s) 1499, 1546, 2944
BceAI ACGGC 5 cut(s) 415, 739, 756, 1172, 2721
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 778, 812
BciT130I CCWGG 7 cut(s) 473, 677, 913, 1502, 1559, 2573, 3037
BclI TGATCA 2 cut(s) 2128, 2913
BcnI CCSGG 5 cut(s) 50, 155, 778, 927, 928
BcoDI GTCTC 1 cut(s) 1046
BcuI ACTAGT 1 cut(s) 2042
BfaI CTAG 6 cut(s) 527, 1056, 1854, 2043, 2363, 2898
BfmI CTRYAG 5 cut(s) 1023, 1535, 1959, 2064, 2682
BfoI RGCGCY 4 cut(s) 597, 624, 657, 755
BfrI CTTAAG 1 cut(s) 1934
BfuAI ACCTGC 1 cut(s) 1759
BlpI GCTNAGC 2 cut(s) 1929, 2224
BmcAI AGTACT 1 cut(s) 1455
Bme18I GGWCC 6 cut(s) 52, 178, 679, 780, 994, 1555
BmeT110I CYCGRG 4 cut(s) 55, 206, 857, 926
BoxI GACNNNNGTC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 550
BplI GAGNNNNNCTC 4 cut(s) 1208, 1240, 2630, 2662
BpmI CTGGAG 5 cut(s) 79, 455, 776, 1419, 2072
Bpu10I CCTNAGC 2 cut(s) 281, 906
Bpu1102I GCTNAGC 2 cut(s) 1929, 2224
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 290, 1019, 2241
BpuMI CCSGG 5 cut(s) 50, 155, 778, 927, 928
BsaAI YACGTR 3 cut(s) 1298, 1694, 2278
BsaHI GRCGYC 4 cut(s) 159, 194, 600, 621
BsaXI ACNNNNNCTCC 2 cut(s) 836, 866
Bse118I RCCGGY 1 cut(s) 584
Bse1I ACTGG 6 cut(s) 565, 640, 1019, 2316, 2787, 2992
Bse21I CCTNAGG 1 cut(s) 2337
Bse3DI GCAATG 4 cut(s) 1194, 1410, 1790, 2800
BseBI CCWGG 7 cut(s) 473, 677, 913, 1502, 1559, 2573, 3037
BseMI GCAATG 4 cut(s) 1194, 1410, 1790, 2800
BseMII CTCAG 9 cut(s) 295, 308, 567, 897, 962, 1023, 1943, 2238, 2328
BseNI ACTGG 6 cut(s) 565, 640, 1019, 2316, 2787, 2992
BseRI GAGGAG 3 cut(s) 224, 308, 2707
BseSI GKGCMC 1 cut(s) 1194
BsgI GTGCAG 2 cut(s) 1459, 2405
Bsh1236I CGCG 1 cut(s) 255
Bsh1285I CGRYCG 1 cut(s) 113
BshNI GGYRCC 2 cut(s) 620, 1492
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 5 cut(s) 387, 1194, 1226, 2217, 2733
BsiHKCI CYCGRG 4 cut(s) 55, 206, 857, 926
BsiSI CCGG 8 cut(s) 50, 155, 238, 419, 585, 777, 927, 2210
BslFI GGGAC 7 cut(s) 515, 680, 793, 838, 1109, 2262, 3014
BsmAI GTCTC 1 cut(s) 1046
BsmFI GGGAC 7 cut(s) 515, 680, 793, 838, 1109, 2262, 3014
BsmI GAATGC 1 cut(s) 2358
BsoBI CYCGRG 4 cut(s) 55, 206, 857, 926
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 6 cut(s) 387, 925, 1194, 1226, 2217, 2733
Bsp143I GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
Bsp1720I GCTNAGC 2 cut(s) 1929, 2224
Bsp19I CCATGG 1 cut(s) 351
Bsp68I TCGCGA 1 cut(s) 255
BspCNI CTCAG 9 cut(s) 294, 307, 566, 898, 961, 1024, 1942, 2237, 2329
BspFNI CGCG 1 cut(s) 255
BspMAI CTGCAG 3 cut(s) 1027, 1963, 2686
BspMI ACCTGC 1 cut(s) 1759
BspPI GGATC 4 cut(s) 5, 1280, 1807, 2869
BspQI GCTCTTC 1 cut(s) 2553
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 2 cut(s) 620, 1492
BspTI CTTAAG 1 cut(s) 1934
BsrDI GCAATG 4 cut(s) 1194, 1410, 1790, 2800
BsrFI RCCGGY 1 cut(s) 584
BsrI ACTGG 6 cut(s) 565, 640, 1019, 2316, 2787, 2992
BssAI RCCGGY 1 cut(s) 584
BssMI GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
BssNI GRCGYC 4 cut(s) 159, 194, 600, 621
BssSI CACGAG 1 cut(s) 1425
BssT1I CCWWGG 4 cut(s) 351, 1870, 2496, 3078
Bst2BI CACGAG 1 cut(s) 1425
Bst2UI CCWGG 7 cut(s) 473, 677, 913, 1502, 1559, 2573, 3037
Bst4CI ACNGT 5 cut(s) 111, 376, 1497, 2014, 3002
Bst6I CTCTTC 4 cut(s) 134, 2160, 2553, 2682
BstACI GRCGYC 4 cut(s) 159, 194, 600, 621
BstAFI CTTAAG 1 cut(s) 1934
BstAPI GCANNNNNTGC 2 cut(s) 1319, 1589
BstBAI YACGTR 3 cut(s) 1298, 1694, 2278
BstBI TTCGAA 1 cut(s) 66
BstDSI CCRYGG 3 cut(s) 351, 738, 2120
BstENI CCTNNNNNAGG 4 cut(s) 661, 1250, 1874, 3057
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 4 cut(s) 597, 624, 657, 755
BstHHI GCGC 8 cut(s) 484, 499, 508, 596, 623, 656, 745, 754
BstKTI GATC 8 cut(s) 13, 768, 1288, 1802, 2131, 2442, 2864, 2916
BstMAI GTCTC 1 cut(s) 1046
BstMBI GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
BstMCI CGRYCG 1 cut(s) 113
BstNI CCWGG 7 cut(s) 473, 677, 913, 1502, 1559, 2573, 3037
BstNSI RCATGY 2 cut(s) 988, 2080
BstPAI GACNNNNGTC 1 cut(s) 57
BstSFI CTRYAG 5 cut(s) 1023, 1535, 1959, 2064, 2682
BstSLI GKGCMC 1 cut(s) 1194
BstSNI TACGTA 1 cut(s) 2278
BstUI CGCG 1 cut(s) 255
BstV2I GAAGAC 1 cut(s) 550
BstX2I RGATCY 3 cut(s) 1285, 1799, 2861
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 3 cut(s) 1285, 1799, 2861
Bsu36I CCTNAGG 1 cut(s) 2337
BtgI CCRYGG 3 cut(s) 351, 738, 2120
BtgZI GCGATG 1 cut(s) 2219
BtsI GCAGTG 3 cut(s) 442, 1020, 2731
BtsIMutI CAGTG 7 cut(s) 442, 572, 647, 1020, 1471, 2309, 2731
BtuMI TCGCGA 1 cut(s) 255
BveI ACCTGC 1 cut(s) 1759
CaiI CAGNNNCTG 6 cut(s) 31, 571, 646, 682, 1031, 1592
CfoI GCGC 8 cut(s) 484, 499, 508, 596, 623, 656, 745, 754
Cfr10I RCCGGY 1 cut(s) 584
Cfr9I CCCGGG 1 cut(s) 926
CseI GACGC 5 cut(s) 167, 183, 436, 840, 1769
CsiI ACCWGGT 1 cut(s) 2571
Csp6I GTAC 8 cut(s) 867, 1238, 1430, 1454, 1493, 1498, 1529, 1891
CviQI GTAC 8 cut(s) 867, 1238, 1430, 1454, 1493, 1498, 1529, 1891
DinI GGCGCC 1 cut(s) 622
DpnI GATC 8 cut(s) 12, 767, 1287, 1801, 2130, 2441, 2863, 2915
DpnII GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
DraIII CACNNNGTG 1 cut(s) 1189
DrdI GACNNNNNNGTC 1 cut(s) 307
DseDI GACNNNNNNGTC 1 cut(s) 307
EaeI YGGCCR 6 cut(s) 3, 33, 354, 416, 774, 879
Eam1104I CTCTTC 4 cut(s) 134, 2160, 2553, 2682
EarI CTCTTC 4 cut(s) 134, 2160, 2553, 2682
Ecl136II GAGCTC 2 cut(s) 385, 2215
Eco105I TACGTA 1 cut(s) 2278
Eco130I CCWWGG 4 cut(s) 351, 1870, 2496, 3078
Eco147I AGGCCT 1 cut(s) 716
Eco24I GRGCYC 3 cut(s) 387, 925, 2217
Eco47I GGWCC 6 cut(s) 52, 178, 679, 780, 994, 1555
Eco53kI GAGCTC 2 cut(s) 385, 2215
Eco57I CTGAAG 4 cut(s) 191, 1431, 1904, 2555
Eco72I CACGTG 1 cut(s) 1298
Eco81I CCTNAGG 1 cut(s) 2337
Eco88I CYCGRG 4 cut(s) 55, 206, 857, 926
EcoICRI GAGCTC 2 cut(s) 385, 2215
EcoNI CCTNNNNNAGG 4 cut(s) 661, 1250, 1874, 3057
EcoO109I RGGNCCY 2 cut(s) 124, 679
EcoRI GAATTC 1 cut(s) 1273
EcoRII CCWGG 7 cut(s) 471, 675, 911, 1500, 1557, 2571, 3035
EcoT14I CCWWGG 4 cut(s) 351, 1870, 2496, 3078
EcoT22I ATGCAT 2 cut(s) 2371, 2429
EcoT38I GRGCYC 3 cut(s) 387, 925, 2217
EgeI GGCGCC 1 cut(s) 622
EheI GGCGCC 1 cut(s) 622
ErhI CCWWGG 4 cut(s) 351, 1870, 2496, 3078
FalI AAGNNNNNCTT 6 cut(s) 2514, 2546, 2547, 2579, 2733, 2765
FaqI GGGAC 7 cut(s) 515, 680, 793, 838, 1109, 2262, 3014
FauI CCCGC 3 cut(s) 221, 284, 540
FauNDI CATATG 1 cut(s) 2956
FbaI TGATCA 2 cut(s) 2128, 2913
FblI GTMKAC 2 cut(s) 197, 299
FriOI GRGCYC 3 cut(s) 387, 925, 2217
FspBI CTAG 6 cut(s) 527, 1056, 1854, 2043, 2363, 2898
FspI TGCGCA 1 cut(s) 498
GlaI GCGC 8 cut(s) 483, 498, 507, 595, 622, 655, 744, 753
GsuI CTGGAG 5 cut(s) 79, 455, 776, 1419, 2072
HaeII RGCGCY 4 cut(s) 597, 624, 657, 755
HapII CCGG 8 cut(s) 50, 155, 238, 419, 585, 777, 927, 2210
HgaI GACGC 5 cut(s) 167, 183, 436, 840, 1769
HhaI GCGC 8 cut(s) 484, 499, 508, 596, 623, 656, 745, 754
Hin1I GRCGYC 4 cut(s) 159, 194, 600, 621
Hin6I GCGC 8 cut(s) 482, 497, 506, 594, 621, 654, 743, 752
HinP1I GCGC 8 cut(s) 482, 497, 506, 594, 621, 654, 743, 752
HincII GTYRAC 4 cut(s) 198, 300, 540, 1338
HindII GTYRAC 4 cut(s) 198, 300, 540, 1338
HindIII AAGCTT 1 cut(s) 1520
HinfI GANTC 7 cut(s) 58, 260, 338, 476, 1595, 1964, 2270
HpaII CCGG 8 cut(s) 50, 155, 238, 419, 585, 777, 927, 2210
HphI GGTGA 8 cut(s) 442, 1061, 1283, 1364, 2591, 2897, 2923, 3074
Hpy166II GTNNAC 6 cut(s) 198, 300, 312, 540, 1192, 1338
Hpy8I GTNNAC 6 cut(s) 198, 300, 312, 540, 1192, 1338
Hpy99I CGWCG 3 cut(s) 199, 260, 602
HpyAV CCTTC 7 cut(s) 857, 1735, 1920, 2102, 2239, 2516, 2530
HpyCH4III ACNGT 5 cut(s) 111, 376, 1497, 2014, 3002
HpyCH4IV ACGT 5 cut(s) 600, 1297, 1693, 2277, 2769
HpySE526I ACGT 5 cut(s) 600, 1297, 1693, 2277, 2769
Hsp92I GRCGYC 4 cut(s) 159, 194, 600, 621
HspAI GCGC 8 cut(s) 482, 497, 506, 594, 621, 654, 743, 752
KasI GGCGCC 1 cut(s) 620
KpnI GGTACC 1 cut(s) 1496
KroI GCCGGC 1 cut(s) 584
KroNI GCCGGC 1 cut(s) 586
Ksp22I TGATCA 2 cut(s) 2128, 2913
Kzo9I GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
LguI GCTCTTC 1 cut(s) 2553
MabI ACCWGGT 1 cut(s) 2571
MaeI CTAG 6 cut(s) 527, 1056, 1854, 2043, 2363, 2898
MaeII ACGT 5 cut(s) 600, 1297, 1693, 2277, 2769
MaeIII GTNAC 6 cut(s) 448, 1599, 1839, 1945, 2072, 2452
MalI GATC 8 cut(s) 12, 767, 1287, 1801, 2130, 2441, 2863, 2915
MboI GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
MboII GAAGA 8 cut(s) 121, 550, 2147, 2189, 2531, 2567, 2570, 2699
MfeI CAATTG 2 cut(s) 806, 1331
MflI RGATCY 3 cut(s) 1285, 1799, 2861
MhlI GDGCHC 6 cut(s) 387, 925, 1194, 1226, 2217, 2733
MlsI TGGCCA 3 cut(s) 35, 356, 881
MluNI TGGCCA 3 cut(s) 35, 356, 881
Mly113I GGCGCC 1 cut(s) 621
MlyI GAGTC 4 cut(s) 67, 254, 485, 2279
MmeI TCCRAC 6 cut(s) 600, 684, 972, 1705, 2165, 2883
Mox20I TGGCCA 3 cut(s) 35, 356, 881
Mph1103I ATGCAT 2 cut(s) 2371, 2429
MroNI GCCGGC 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 1277
MscI TGGCCA 3 cut(s) 35, 356, 881
MseI TTAA 8 cut(s) 1317, 1571, 1662, 1935, 2037, 2102, 2601, 2751
MslI CAYNNNNRTG 4 cut(s) 977, 1296, 2003, 2736
Msp20I TGGCCA 3 cut(s) 35, 356, 881
MspA1I CMGCKG 2 cut(s) 31, 2195
MspCI CTTAAG 1 cut(s) 1934
MspI CCGG 8 cut(s) 50, 155, 238, 419, 585, 777, 927, 2210
MunI CAATTG 2 cut(s) 806, 1331
Mva1269I GAATGC 1 cut(s) 2358
MvaI CCWGG 7 cut(s) 473, 677, 913, 1502, 1559, 2573, 3037
MvnI CGCG 1 cut(s) 255
NaeI GCCGGC 1 cut(s) 586
NarI GGCGCC 1 cut(s) 621
NciI CCSGG 5 cut(s) 50, 155, 778, 927, 928
NcoI CCATGG 1 cut(s) 351
NdeI CATATG 1 cut(s) 2956
NdeII GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
NgoMIV GCCGGC 1 cut(s) 584
NmeAIII GCCGAG 4 cut(s) 145, 597, 813, 1240
NmuCI GTSAC 2 cut(s) 448, 1599
NruI TCGCGA 1 cut(s) 255
NsbI TGCGCA 1 cut(s) 498
NsiI ATGCAT 2 cut(s) 2371, 2429
NspI RCATGY 2 cut(s) 988, 2080
NspV TTCGAA 1 cut(s) 66
OliI CACNNNNGTG 2 cut(s) 1296, 2736
PaeR7I CTCGAG 1 cut(s) 857
PceI AGGCCT 1 cut(s) 716
PciI ACATGT 1 cut(s) 984
PciSI GCTCTTC 1 cut(s) 2553
PcsI WCGNNNNNNNCGW 1 cut(s) 1690
PctI GAATGC 1 cut(s) 2358
PdiI GCCGGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 1277
PfeI GAWTC 3 cut(s) 338, 1595, 1964
PfoI TCCNGGA 3 cut(s) 471, 675, 1557
PleI GAGTC 4 cut(s) 66, 254, 484, 2278
PluTI GGCGCC 1 cut(s) 624
PmaCI CACGTG 1 cut(s) 1298
PmlI CACGTG 1 cut(s) 1298
PpsI GAGTC 4 cut(s) 66, 254, 484, 2278
Ppu21I YACGTR 3 cut(s) 1298, 1694, 2278
PpuMI RGGWCCY 1 cut(s) 679
PscI ACATGT 1 cut(s) 984
PshAI GACNNNNGTC 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 1317
Psp124BI GAGCTC 2 cut(s) 387, 2217
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 7 cut(s) 471, 675, 911, 1500, 1557, 2571, 3035
PspCI CACGTG 1 cut(s) 1298
PspGI CCWGG 7 cut(s) 471, 675, 911, 1500, 1557, 2571, 3035
PspPPI RGGWCCY 1 cut(s) 679
PspXI VCTCGAGB 1 cut(s) 857
PstI CTGCAG 3 cut(s) 1027, 1963, 2686
PstNI CAGNNNCTG 6 cut(s) 31, 571, 646, 682, 1031, 1592
PsuI RGATCY 3 cut(s) 1285, 1799, 2861
PvuII CAGCTG 2 cut(s) 31, 2195
RruI TCGCGA 1 cut(s) 255
RsaI GTAC 8 cut(s) 868, 1239, 1431, 1455, 1494, 1499, 1530, 1892
RsaNI GTAC 8 cut(s) 867, 1238, 1430, 1454, 1493, 1498, 1529, 1891
RseI CAYNNNNRTG 4 cut(s) 977, 1296, 2003, 2736
SacI GAGCTC 2 cut(s) 387, 2217
SalI GTCGAC 2 cut(s) 196, 298
SapI GCTCTTC 1 cut(s) 2553
SaqAI TTAA 8 cut(s) 1317, 1571, 1662, 1935, 2037, 2102, 2601, 2751
Sau3AI GATC 8 cut(s) 10, 765, 1285, 1799, 2128, 2439, 2861, 2913
ScaI AGTACT 1 cut(s) 1455
SchI GAGTC 4 cut(s) 67, 254, 485, 2279
SduI GDGCHC 6 cut(s) 387, 925, 1194, 1226, 2217, 2733
SexAI ACCWGGT 1 cut(s) 2571
SfcI CTRYAG 5 cut(s) 1023, 1535, 1959, 2064, 2682
SfoI GGCGCC 1 cut(s) 622
Sfr274I CTCGAG 1 cut(s) 857
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 6 cut(s) 52, 178, 679, 780, 994, 1555
SlaI CTCGAG 1 cut(s) 857
SmaI CCCGGG 1 cut(s) 928
SmiMI CAYNNNNRTG 4 cut(s) 977, 1296, 2003, 2736
SmlI CTYRAG 5 cut(s) 305, 857, 998, 1934, 2256
SmoI CTYRAG 5 cut(s) 305, 857, 998, 1934, 2256
SnaBI TACGTA 1 cut(s) 2278
SpeI ACTAGT 1 cut(s) 2042
SseBI AGGCCT 1 cut(s) 716
SspDI GGCGCC 1 cut(s) 620
SspI AATATT 1 cut(s) 2756
SspMI CTAG 6 cut(s) 527, 1056, 1854, 2043, 2363, 2898
SstI GAGCTC 2 cut(s) 387, 2217
StuI AGGCCT 1 cut(s) 716
StyI CCWWGG 4 cut(s) 351, 1870, 2496, 3078
TaaI ACNGT 5 cut(s) 111, 376, 1497, 2014, 3002
TaiI ACGT 5 cut(s) 603, 1300, 1696, 2280, 2772
TaqI TCGA 7 cut(s) 66, 197, 299, 768, 858, 1248, 2422
TaqII GACCGA 1 cut(s) 797
TatI WGTACW 3 cut(s) 1429, 1453, 1528
TauI GCSGC 5 cut(s) 8, 244, 584, 1113, 1445
TfiI GAWTC 3 cut(s) 338, 1595, 1964
Tru1I TTAA 8 cut(s) 1317, 1571, 1662, 1935, 2037, 2102, 2601, 2751
Tru9I TTAA 8 cut(s) 1317, 1571, 1662, 1935, 2037, 2102, 2601, 2751
TscAI CASTG 7 cut(s) 449, 572, 647, 1027, 1471, 2316, 2738
TseFI GTSAC 2 cut(s) 448, 1599
Tsp45I GTSAC 2 cut(s) 448, 1599
TspGWI ACGGA 4 cut(s) 214, 725, 1137, 1355
TspMI CCCGGG 1 cut(s) 926
TspRI CASTG 7 cut(s) 449, 572, 647, 1027, 1471, 2316, 2738
Vha464I CTTAAG 1 cut(s) 1934
VneI GTGCAC 1 cut(s) 1190
VpaK11BI GGWCC 6 cut(s) 52, 178, 679, 780, 994, 1555
VspI ATTAAT 1 cut(s) 1317
XagI CCTNNNNNAGG 4 cut(s) 661, 1250, 1874, 3057
XapI RAATTY 3 cut(s) 1273, 2162, 2346
XbaI TCTAGA 1 cut(s) 2362
XceI RCATGY 2 cut(s) 988, 2080
XcmI CCANNNNNNNNNTGG 2 cut(s) 349, 1302
XhoI CTCGAG 1 cut(s) 857
XmaI CCCGGG 1 cut(s) 926
XmiI GTMKAC 2 cut(s) 197, 299
XmnI GAANNNNTTC 1 cut(s) 1277
XspI CTAG 6 cut(s) 527, 1056, 1854, 2043, 2363, 2898
ZraI GACGTC 1 cut(s) 601
ZrmI AGTACT 1 cut(s) 1455
Zsp2I ATGCAT 2 cut(s) 2371, 2429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.