Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000344

Physical Location & Seq

Genomic Coordinates Reverse (-)

35807097 .. 35812319

Gene Structure 5223 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1225200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGGCCGAGTCGACTCAGTTACAGCAAGCCCAACTGGCGACGATTCTCGGTCCCGACCCGGCCCCTTTCCAAACCCTGATCTCCCACCTCATGTCTTCCGCCAACGATCAGCGCTCCCAGGCGGAGCTCCTCTTCAACCTCTGCAAGCAGACCGATCCCGACTCCCTCTCCCTCAAGCTCGCCCACCTCATCCAGTTCTCCCCCGCCGCCGAGGCCCGCGCCATGTCCGCGATTCTCCTCCGCAAGCAGCTCACCCGCAACGACTCGTATCTCTGGCCCCGATTGAATCCGACGACCCAATCAACGCTGAAGACGATTCTGCTGACGTGTATTCAGCAGGAGGGCACGAAATCCATCTCCAAGAAGCTCTGCGATACGATTTCCGAGCTCGCCTCTGGGATTTTACCCGATAACGGATGGCCGGAGCTGCTGCCGTTTATGTTCCAGTGCGTCTCGTCTGATTCGCCAAAGCTTCAAGAAGCGGCGTTCTTGATATTCGCTCAGTTATCGCAGTACATCGGCGACATCATGGTGCCGCACATAAAAGAATTGCACGCCGTGTTCTTGCATAGCCTGGGGAATTCGCCGAGCCCCGACGTGAAGATCGCGGCTTTGAACGCCGTCATCAATTTCATTCAGTGTTTGACCAGCTCTGCGGATAGGGATAGGTTTCAGGACCTGTTGCCGGCGATGATGAAGACGTTGATGGAGGCTCTGAACAACGGGAACGAAGCCACCGCGCAGGACGCTCTTGAATTGTTGATTGAATTGGCTGGGACTGAGCCGAGGTTCCTAAGGCGGCAGATTGTGGAAGTCGTTGGCGCAATGTTACAGATTGCTGAGGCCGATTCCTTGGAAGAATCAACAAGGCACTTGGCCATTGAGTTCGTCATCACTCTGGCTGAGGCCAGAGAGCGTGCTCCTGGCATGATGAGGAAGTTGCCGCAGTTTATAAGCCGATTGTTTGCCATTTTAATGAAAATGCTTCTGGATATTGAGGACGAACCGGCATGGCATGCGGCAGAGAGTGAAGATGAGGATGCCGGTGAGACCACTAATTACAGTGTTGGTCAAGAGTGTTTGGATCGGCTTTCGATTTCCCTTGGTGGGAATACCATAGTTCCAGTAGCTTCTGAGCAATTGCCTGCATATTTGGTTGCCCCTGAGTGGCAGAAGCACCATGCTGCATTGATTGCCCTTGCTCAGATTGCTGAGGGTTGTGCTAAGGTAATGATTAAAAATTTGGAGCAAGTGGTAGCAATGGTCTTGAACTCTTTTCAAGATCCACATCCTCGTGTAAGGTGGGCAGCGATTAATGCAATTGGGCAGTTGTCTACTGATTTGGGCCCAGATCTGCAAGTTCAATATCACCAAAGTGTGCTCCCAGCATTGGCTGGAGCTATGGATGATTTTCAGAACCCTCGTGTTCAGGCACATGCTGCTTCGGCTGTGCTGAATTTCAGTGAGAATTGCACTCCGGATATTTTAACACCGTATTTGGATGGAGTAGTGAGCAAATTGCTTGTACTTCTGCAGAACGGAAAACAAATGGTGCAAGAGGGTGCCTTGACTGCTTTGGCATCCGTTGCTGATTCATCTCAGGAGCATTTTCAAAAATATTATGATGCAGTTATGCCTTACCTGAAAGCTATCTTGGTGAATGCCACTGATAAGTCTAATCGCATGCTTCGTGCCAAATCGATGGAGTGTATTAGTTTGGTTGGAATGGCAGTTGGAAAAGAGAAATTCAGGGAAGACGCTAAGCAAGTCATGGAAGTGCTGATGGCTTTGCAAGGATCTCACATGGAGACGGATGATCCAACTACAAGTTACATGTTACAAGCCTGGGCTAGACTCTGCAAGTGCTTAGGACAAGATTTCCTCCCATATATGAGTGTTGTAATGCCTCCTTTGCTTCAGTCTGCTCAACTTAAGCCAGATGTAACCATCACATCTGCAGATGACGACAATGATATTGATGACTCTGATGATGATAGTATGGAAACAATTACTCTTGGGGATAAAAGGATTGGAATCAAGACTAGTGTCTTGGAGGAGAAAGCTACAGCTTGCAATATGCTGTGTTGCTATGCTGATGAGCTGAAAGAAGGATTCTTTCCCTGGATTGATCAGGTTGCCCCCACTTTGGTTCCACTTCTTAAATTTTATTTCCATGAAGAAGTTAGGAAGGCAGCTGTTTCAGCAATGCCGGAGCTACTGCGTTCTGCAAAGTTAGCTATAGAGAAAGGCCAAGCTCAAGGTCGTAACGAAACATATATAAAGCAATTGTCTGACTACATTGTTCCAGCTCTTGTGGAAGCATTACATAAGGAACCTGATACTGAAATCTGTGCAAATATATTGGATGCATTAAATGAATGCTTACAGGTTTGTCATTTTTCTTTAAGAAATTGTATGAATTTTCCATTGTGGCCCTGTTTATTTTACTTTTTGCACTTACTCCGCACCAACCGACCAATTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0001101 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0031090 GO:0031965 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032386 GO:0032388 GO:0032879 GO:0032880 GO:0033036 GO:0033157 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042221 GO:0042277 GO:0042306 GO:0042307 GO:0042886 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046822 GO:0046824 GO:0046907 GO:0048518 GO:0050789 GO:0050896 GO:0051049 GO:0051050 GO:0051169 GO:0051170 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0060341 GO:0065007 GO:0070013 GO:0070201 GO:0070727 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071702 GO:0071705 GO:0072594 GO:0090087 GO:0090316 GO:1900180 GO:1900182 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1903827 GO:1903829 GO:1904589 GO:1904591 GO:1904951

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT HEAT_2 HEAT_EZ

Protein Analysis

829

Amino Acids

91.81

Weight (kDa)

4.92

Isoelectric Point (pI)

50.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_IPO5	PF25780	117 - 273	1.3e-35	IPO5-like, TPR repeats
Importin_rep_4	PF18808	284 - 375	1.2e-21	Importin repeat
HEAT_2	PF13646	387 - 483	3e-06	HEAT repeats
HEAT_EZ	PF13513	390 - 444	2.9e-08	HEAT-like repeat
TOR1L1_N	PF24714	400 - 592	1.6e-08	TORTIFOLIA1 N-terminal
HEAT_EZ	PF13513	474 - 528	9.1e-07	HEAT-like repeat
TPR_IMB1	PF25574	477 - 646	1.8e-11	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000344)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19820
fragaria_vesca	FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica	MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica	Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis	pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis	RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata	RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora	Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii	Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa	Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha	Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana	Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	953
AccB1I	GGYRCC	2 cut(s)	532, 1562
AccI	GTMKAC	2 cut(s)	11, 1334
AccII	CGCG	4 cut(s)	219, 230, 608, 740
AccIII	TCCGGA	1 cut(s)	1477
AclWI	GGATC	5 cut(s)	149, 1092, 1277, 1804, 1811
AcoI	YGGCCR	3 cut(s)	3, 419, 876
AcsI	RAATTY	6 cut(s)	580, 1240, 1456, 1745, 2162, 2419
AcuI	CTGAAG	2 cut(s)	329, 1901
AdeI	CACNNNGTG	1 cut(s)	559
AfaI	GTAC	2 cut(s)	515, 1527
AfeI	AGCGCT	1 cut(s)	113
AfiI	CCNNNNNNNGG	6 cut(s)	413, 685, 1107, 1167, 1390, 2146
AflII	CTTAAG	1 cut(s)	1931
AflIII	ACRYGT	2 cut(s)	326, 1833
AhlI	ACTAGT	1 cut(s)	2042
AjiI	CACGTC	2 cut(s)	327, 598
AjnI	CCWGG	5 cut(s)	117, 573, 922, 1844, 2120
AjuI	GAANNNNNNNTTGG	2 cut(s)	1637, 1669
AleI	CACNNNNGTG	1 cut(s)	1374
Alw21I	GWGCWC	4 cut(s)	129, 390, 922, 1383
Alw26I	GTCTC	3 cut(s)	457, 1043, 1802
AlwI	GGATC	5 cut(s)	149, 1092, 1277, 1804, 1811
AlwNI	CAGNNNCTG	1 cut(s)	679
Aor13HI	TCCGGA	1 cut(s)	1477
Aor51HI	AGCGCT	1 cut(s)	113
ApaI	GGGCCC	1 cut(s)	1349
ApeKI	GCWGC	7 cut(s)	247, 427, 430, 1184, 1307, 1439, 2192
ApoI	RAATTY	6 cut(s)	580, 1240, 1456, 1745, 2162, 2419
AseI	ATTAAT	1 cut(s)	1314
AspLEI	GCGC	4 cut(s)	114, 221, 742, 824
AspS9I	GGNCC	8 cut(s)	50, 61, 214, 276, 676, 1345, 1346, 2433
AsuC2I	CCSGG	1 cut(s)	59
AsuHPI	GGTGA	4 cut(s)	244, 1058, 1361, 1669
AvaII	GGWCC	2 cut(s)	50, 676
AxyI	CCTNAGG	1 cut(s)	794
BaeGI	GKGCMC	2 cut(s)	347, 1349
BalI	TGGCCA	1 cut(s)	878
BanI	GGYRCC	2 cut(s)	532, 1562
BanII	GRGCYC	4 cut(s)	129, 390, 593, 1349
BauI	CACGAG	2 cut(s)	1293, 1422
BbsI	GAAGAC	4 cut(s)	87, 317, 704, 1761
Bbv12I	GWGCWC	4 cut(s)	129, 390, 922, 1383
BbvCI	CCTCAGC	3 cut(s)	840, 903, 1212
BbvI	GCAGC	7 cut(s)	259, 414, 417, 1171, 1319, 1426, 2204
BccI	CCATC	7 cut(s)	362, 411, 700, 1496, 1696, 1777, 1955
BceAI	ACGGC	3 cut(s)	418, 542, 605
BciT130I	CCWGG	5 cut(s)	119, 575, 924, 1846, 2122
BclI	TGATCA	1 cut(s)	2128
BcnI	CCSGG	1 cut(s)	59
BcoDI	GTCTC	3 cut(s)	457, 1043, 1802
BcuI	ACTAGT	1 cut(s)	2042
BfaI	CTAG	2 cut(s)	1851, 2043
BfmI	CTRYAG	4 cut(s)	1532, 1956, 2064, 2238
BfoI	RGCGCY	1 cut(s)	115
BfrI	CTTAAG	1 cut(s)	1931
BglI	GCCNNNNNGGC	2 cut(s)	35, 212
BglII	AGATCT	1 cut(s)	1351
BlpI	GCTNAGC	1 cut(s)	1761
Bme1390I	CCNGG	6 cut(s)	59, 119, 575, 924, 1846, 2122
Bme18I	GGWCC	2 cut(s)	50, 676
BmgBI	CACGTC	2 cut(s)	327, 598
BmgT120I	GGNCC	8 cut(s)	50, 61, 214, 276, 676, 1345, 1346, 2433
BmiI	GGNNCC	9 cut(s)	52, 63, 278, 534, 791, 1347, 1564, 2151, 2334
BmrFI	CCNGG	6 cut(s)	59, 119, 575, 924, 1846, 2122
BmsI	GCATC	4 cut(s)	1030, 1589, 1615, 2356
BoxI	GACNNNNGTC	1 cut(s)	2045
BpiI	GAAGAC	4 cut(s)	87, 317, 704, 1761
BplI	GAGNNNNNCTC	2 cut(s)	377, 409
BpmI	CTGGAG	1 cut(s)	1416
Bpu10I	CCTNAGC	5 cut(s)	840, 903, 1212, 1224, 1867
Bpu1102I	GCTNAGC	1 cut(s)	1761
BpuEI	CTTGAG	2 cut(s)	158, 2241
BpuMI	CCSGG	1 cut(s)	59
Bsa29I	ATCGAT	1 cut(s)	1700
BsaBI	GATNNNNATC	2 cut(s)	1287, 2033
BsaI	GGTCTC	1 cut(s)	1043
BsaJI	CCNNGG	8 cut(s)	117, 210, 574, 785, 852, 1102, 1845, 2120
BsaWI	WCCGGW	1 cut(s)	1477
BsaXI	ACNNNNNCTCC	2 cut(s)	152, 182
Bsc4I	CCNNNNNNNGG	6 cut(s)	413, 685, 1107, 1167, 1390, 2146
Bse118I	RCCGGY	3 cut(s)	685, 1006, 1043
Bse1I	ACTGG	4 cut(s)	39, 193, 445, 1124
Bse21I	CCTNAGG	1 cut(s)	794
Bse3DI	GCAATG	3 cut(s)	831, 1266, 2211
Bse8I	GATNNNNATC	2 cut(s)	1287, 2033
BseAI	TCCGGA	1 cut(s)	1477
BseBI	CCWGG	5 cut(s)	119, 575, 924, 1846, 2122
BseCI	ATCGAT	1 cut(s)	1700
BseDI	CCNNGG	8 cut(s)	117, 210, 574, 785, 852, 1102, 1845, 2120
BseGI	GGATG	9 cut(s)	189, 422, 1045, 1288, 1411, 1507, 1580, 1819, 2371
BseJI	GATNNNNATC	2 cut(s)	1287, 2033
BseLI	CCNNNNNNNGG	6 cut(s)	413, 685, 1107, 1167, 1390, 2146
BseMI	GCAATG	3 cut(s)	831, 1266, 2211
BseNI	ACTGG	4 cut(s)	39, 193, 445, 1124
BseRI	GAGGAG	3 cut(s)	119, 227, 2069
BseSI	GKGCMC	2 cut(s)	347, 1349
BseXI	GCAGC	7 cut(s)	259, 414, 417, 1171, 1319, 1426, 2204
BseYI	CCCAGC	2 cut(s)	773, 1384
Bsh1236I	CGCG	4 cut(s)	219, 230, 608, 740
BshNI	GGYRCC	2 cut(s)	532, 1562
BshVI	ATCGAT	1 cut(s)	1700
BsiHKAI	GWGCWC	4 cut(s)	129, 390, 922, 1383
BsiSI	CCGG	7 cut(s)	59, 422, 686, 1007, 1044, 1478, 2210
BslFI	GGGAC	2 cut(s)	36, 790
BslI	CCNNNNNNNGG	6 cut(s)	413, 685, 1107, 1167, 1390, 2146
BsmAI	GTCTC	3 cut(s)	457, 1043, 1802
BsmBI	CGTCTC	2 cut(s)	457, 1802
BsmFI	GGGAC	2 cut(s)	36, 790
BsmI	GAATGC	2 cut(s)	1666, 2384
Bso31I	GGTCTC	1 cut(s)	1043
Bsp120I	GGGCCC	1 cut(s)	1345
Bsp1286I	GDGCHC	7 cut(s)	129, 347, 390, 593, 922, 1349, 1383
Bsp13I	TCCGGA	1 cut(s)	1477
Bsp1720I	GCTNAGC	1 cut(s)	1761
BspDI	ATCGAT	1 cut(s)	1700
BspEI	TCCGGA	1 cut(s)	1477
BspFNI	CGCG	4 cut(s)	219, 230, 608, 740
BspLI	GGNNCC	9 cut(s)	52, 63, 278, 534, 791, 1347, 1564, 2151, 2334
BspMAI	CTGCAG	2 cut(s)	1536, 1960
BspPI	GGATC	5 cut(s)	149, 1092, 1277, 1804, 1811
BspT107I	GGYRCC	2 cut(s)	532, 1562
BspTI	CTTAAG	1 cut(s)	1931
BspTNI	GGTCTC	1 cut(s)	1043
BsrDI	GCAATG	3 cut(s)	831, 1266, 2211
BsrFI	RCCGGY	3 cut(s)	685, 1006, 1043
BsrI	ACTGG	4 cut(s)	39, 193, 445, 1124
BssAI	RCCGGY	3 cut(s)	685, 1006, 1043
BssECI	CCNNGG	8 cut(s)	117, 210, 574, 785, 852, 1102, 1845, 2120
BssSI	CACGAG	2 cut(s)	1293, 1422
BssT1I	CCWWGG	2 cut(s)	852, 1102
Bst2BI	CACGAG	2 cut(s)	1293, 1422
Bst2UI	CCWGG	5 cut(s)	119, 575, 924, 1846, 2122
Bst4CI	ACNGT	2 cut(s)	1064, 1494
Bst6I	CTCTTC	1 cut(s)	137
BstAFI	CTTAAG	1 cut(s)	1931
BstAPI	GCANNNNNTGC	4 cut(s)	1016, 1193, 1439, 1586
BstF5I	GGATG	9 cut(s)	189, 422, 1045, 1288, 1411, 1507, 1580, 1819, 2371
BstFNI	CGCG	4 cut(s)	219, 230, 608, 740
BstH2I	RGCGCY	1 cut(s)	115
BstHHI	GCGC	4 cut(s)	114, 221, 742, 824
BstMAI	GTCTC	3 cut(s)	457, 1043, 1802
BstNI	CCWGG	5 cut(s)	119, 575, 924, 1846, 2122
BstNSI	RCATGY	4 cut(s)	1019, 1439, 1687, 1837
BstPAI	GACNNNNGTC	1 cut(s)	2045
BstSCI	CCNGG	6 cut(s)	57, 117, 573, 922, 1844, 2120
BstSFI	CTRYAG	4 cut(s)	1532, 1956, 2064, 2238
BstSLI	GKGCMC	2 cut(s)	347, 1349
BstUI	CGCG	4 cut(s)	219, 230, 608, 740
BstV1I	GCAGC	7 cut(s)	259, 414, 417, 1171, 1319, 1426, 2204
BstV2I	GAAGAC	4 cut(s)	87, 317, 704, 1761
BstX2I	RGATCY	3 cut(s)	1282, 1351, 1796
BstXI	CCANNNNNNTGG	1 cut(s)	1702
BstYI	RGATCY	3 cut(s)	1282, 1351, 1796
Bsu15I	ATCGAT	1 cut(s)	1700
Bsu36I	CCTNAGG	1 cut(s)	794
BsuTUI	ATCGAT	1 cut(s)	1700
BtgZI	GCGATG	1 cut(s)	704
BtrI	CACGTC	2 cut(s)	327, 598
BtsCI	GGATG	9 cut(s)	189, 422, 1045, 1288, 1411, 1507, 1580, 1819, 2371
BtsIMutI	CAGTG	5 cut(s)	452, 644, 1069, 1468, 1665
CaiI	CAGNNNCTG	1 cut(s)	679
CfoI	GCGC	4 cut(s)	114, 221, 742, 824
Cfr10I	RCCGGY	3 cut(s)	685, 1006, 1043
Cfr13I	GGNCC	8 cut(s)	50, 61, 214, 276, 676, 1345, 1346, 2433
ClaI	ATCGAT	1 cut(s)	1700
CseI	GACGC	3 cut(s)	439, 755, 1766
Csp6I	GTAC	2 cut(s)	514, 1526
CviQI	GTAC	2 cut(s)	514, 1526
DraIII	CACNNNGTG	1 cut(s)	559
EaeI	YGGCCR	3 cut(s)	3, 419, 876
Eam1104I	CTCTTC	1 cut(s)	137
EarI	CTCTTC	1 cut(s)	137
EciI	GGCGGA	2 cut(s)	88, 137
Ecl136II	GAGCTC	2 cut(s)	127, 388
Eco130I	CCWWGG	2 cut(s)	852, 1102
Eco24I	GRGCYC	4 cut(s)	129, 390, 593, 1349
Eco31I	GGTCTC	1 cut(s)	1043
Eco47I	GGWCC	2 cut(s)	50, 676
Eco47III	AGCGCT	1 cut(s)	113
Eco53kI	GAGCTC	2 cut(s)	127, 388
Eco57I	CTGAAG	2 cut(s)	329, 1901
Eco81I	CCTNAGG	1 cut(s)	794
EcoICRI	GAGCTC	2 cut(s)	127, 388
EcoO109I	RGGNCCY	1 cut(s)	676
EcoRI	GAATTC	1 cut(s)	580
EcoRII	CCWGG	5 cut(s)	117, 573, 922, 1844, 2120
EcoT14I	CCWWGG	2 cut(s)	852, 1102
EcoT22I	ATGCAT	1 cut(s)	2371
EcoT38I	GRGCYC	4 cut(s)	129, 390, 593, 1349
ErhI	CCWWGG	2 cut(s)	852, 1102
Esp3I	CGTCTC	2 cut(s)	457, 1802
FaqI	GGGAC	2 cut(s)	36, 790
FauI	CCCGC	3 cut(s)	211, 224, 263
FbaI	TGATCA	1 cut(s)	2128
FblI	GTMKAC	2 cut(s)	11, 1334
FokI	GGATG	9 cut(s)	176, 429, 1052, 1275, 1418, 1514, 1567, 1826, 2378
FriOI	GRGCYC	4 cut(s)	129, 390, 593, 1349
FspBI	CTAG	2 cut(s)	1851, 2043
GlaI	GCGC	4 cut(s)	113, 220, 741, 823
GsaI	CCCAGC	2 cut(s)	777, 1388
GsuI	CTGGAG	1 cut(s)	1416
HaeII	RGCGCY	1 cut(s)	115
HapII	CCGG	7 cut(s)	59, 422, 686, 1007, 1044, 1478, 2210
HgaI	GACGC	3 cut(s)	439, 755, 1766
HhaI	GCGC	4 cut(s)	114, 221, 742, 824
Hin6I	GCGC	4 cut(s)	112, 219, 740, 822
HinP1I	GCGC	4 cut(s)	112, 219, 740, 822
HincII	GTYRAC	1 cut(s)	12
HindII	GTYRAC	1 cut(s)	12
HindIII	AAGCTT	1 cut(s)	470
HpaII	CCGG	7 cut(s)	59, 422, 686, 1007, 1044, 1478, 2210
HphI	GGTGA	4 cut(s)	244, 1058, 1361, 1669
Hpy166II	GTNNAC	2 cut(s)	12, 1335
Hpy188I	TCNGA	9 cut(s)	291, 385, 460, 717, 1135, 1206, 1416, 1987, 2293
Hpy8I	GTNNAC	2 cut(s)	12, 1335
Hpy99I	CGWCG	3 cut(s)	43, 295, 599
HpyAV	CCTTC	2 cut(s)	2102, 2182
HpyCH4III	ACNGT	2 cut(s)	1064, 1494
HpyCH4IV	ACGT	3 cut(s)	326, 597, 701
HpySE526I	ACGT	3 cut(s)	326, 597, 701
HspAI	GCGC	4 cut(s)	112, 219, 740, 822
Kpn2I	TCCGGA	1 cut(s)	1477
KroI	GCCGGC	1 cut(s)	685
KroNI	GCCGGC	1 cut(s)	687
Ksp22I	TGATCA	1 cut(s)	2128
Lsp1109I	GCAGC	7 cut(s)	259, 414, 417, 1171, 1319, 1426, 2204
LweI	GCATC	4 cut(s)	1030, 1589, 1615, 2356
MaeI	CTAG	2 cut(s)	1851, 2043
MaeII	ACGT	3 cut(s)	326, 597, 701
MaeIII	GTNAC	6 cut(s)	18, 828, 1829, 1836, 1942, 2264
MboII	GAAGA	9 cut(s)	87, 124, 322, 613, 709, 869, 1043, 1766, 2189
MfeI	CAATTG	3 cut(s)	1139, 1320, 2285
MflI	RGATCY	3 cut(s)	1282, 1351, 1796
MhlI	GDGCHC	7 cut(s)	129, 347, 390, 593, 922, 1349, 1383
MlsI	TGGCCA	1 cut(s)	878
MluNI	TGGCCA	1 cut(s)	878
MlyI	GAGTC	6 cut(s)	7, 17, 155, 257, 1848, 1976
MmeI	TCCRAC	4 cut(s)	314, 1702, 1714, 1844
Mox20I	TGGCCA	1 cut(s)	878
Mph1103I	ATGCAT	1 cut(s)	2371
MroI	TCCGGA	1 cut(s)	1477
MroNI	GCCGGC	1 cut(s)	685
MscI	TGGCCA	1 cut(s)	878
MseI	TTAA	8 cut(s)	974, 1236, 1314, 1487, 1932, 2160, 2372, 2405
MslI	CAYNNNNRTG	5 cut(s)	530, 974, 1293, 1374, 1775
Msp20I	TGGCCA	1 cut(s)	878
MspA1I	CMGCKG	1 cut(s)	2195
MspCI	CTTAAG	1 cut(s)	1931
MspI	CCGG	7 cut(s)	59, 422, 686, 1007, 1044, 1478, 2210
MspR9I	CCNGG	6 cut(s)	59, 119, 575, 924, 1846, 2122
MunI	CAATTG	3 cut(s)	1139, 1320, 2285
Mva1269I	GAATGC	2 cut(s)	1666, 2384
MvaI	CCWGG	5 cut(s)	119, 575, 924, 1846, 2122
MvnI	CGCG	4 cut(s)	219, 230, 608, 740
NaeI	GCCGGC	1 cut(s)	687
NciI	CCSGG	1 cut(s)	59
NgoMIV	GCCGGC	1 cut(s)	685
NlaIV	GGNNCC	9 cut(s)	52, 63, 278, 534, 791, 1347, 1564, 2151, 2334
NmeAIII	GCCGAG	4 cut(s)	31, 235, 612, 810
NsiI	ATGCAT	1 cut(s)	2371
NspI	RCATGY	4 cut(s)	1019, 1439, 1687, 1837
OliI	CACNNNNGTG	1 cut(s)	1374
PaeI	GCATGC	2 cut(s)	1019, 1687
PciI	ACATGT	1 cut(s)	1833
PcsI	WCGNNNNNNNCGW	2 cut(s)	311, 1687
PctI	GAATGC	2 cut(s)	1666, 2384
PdiI	GCCGGC	1 cut(s)	687
PleI	GAGTC	6 cut(s)	7, 16, 155, 257, 1848, 1976
PpsI	GAGTC	6 cut(s)	7, 16, 155, 257, 1848, 1976
PpuMI	RGGWCCY	1 cut(s)	676
PscI	ACATGT	1 cut(s)	1833
PshAI	GACNNNNGTC	1 cut(s)	2045
PshBI	ATTAAT	1 cut(s)	1314
PsiI	TTATAA	1 cut(s)	953
Psp124BI	GAGCTC	2 cut(s)	129, 390
Psp5II	RGGWCCY	1 cut(s)	676
Psp6I	CCWGG	5 cut(s)	117, 573, 922, 1844, 2120
PspFI	CCCAGC	2 cut(s)	773, 1384
PspGI	CCWGG	5 cut(s)	117, 573, 922, 1844, 2120
PspN4I	GGNNCC	9 cut(s)	52, 63, 278, 534, 791, 1347, 1564, 2151, 2334
PspOMI	GGGCCC	1 cut(s)	1345
PspPI	GGNCC	8 cut(s)	50, 61, 214, 276, 676, 1345, 1346, 2433
PspPPI	RGGWCCY	1 cut(s)	676
PstI	CTGCAG	2 cut(s)	1536, 1960
PstNI	CAGNNNCTG	1 cut(s)	679
PsuI	RGATCY	3 cut(s)	1282, 1351, 1796
PvuII	CAGCTG	1 cut(s)	2195
RsaI	GTAC	2 cut(s)	515, 1527
RsaNI	GTAC	2 cut(s)	514, 1526
RseI	CAYNNNNRTG	5 cut(s)	530, 974, 1293, 1374, 1775
SacI	GAGCTC	2 cut(s)	129, 390
SalI	GTCGAC	1 cut(s)	10
SaqAI	TTAA	8 cut(s)	974, 1236, 1314, 1487, 1932, 2160, 2372, 2405
Sau96I	GGNCC	8 cut(s)	50, 61, 214, 276, 676, 1345, 1346, 2433
SchI	GAGTC	6 cut(s)	7, 17, 155, 257, 1848, 1976
ScrFI	CCNGG	6 cut(s)	59, 119, 575, 924, 1846, 2122
SduI	GDGCHC	7 cut(s)	129, 347, 390, 593, 922, 1349, 1383
SfaNI	GCATC	4 cut(s)	1030, 1589, 1615, 2356
SfcI	CTRYAG	4 cut(s)	1532, 1956, 2064, 2238
SinI	GGWCC	2 cut(s)	50, 676
SmiMI	CAYNNNNRTG	5 cut(s)	530, 974, 1293, 1374, 1775
SmlI	CTYRAG	3 cut(s)	173, 1931, 2256
SmoI	CTYRAG	3 cut(s)	173, 1931, 2256
SpeI	ACTAGT	1 cut(s)	2042
SphI	GCATGC	2 cut(s)	1019, 1687
SspI	AATATT	1 cut(s)	1619
SspMI	CTAG	2 cut(s)	1851, 2043
SstI	GAGCTC	2 cut(s)	129, 390
StyD4I	CCNGG	6 cut(s)	57, 117, 573, 922, 1844, 2120
StyI	CCWWGG	2 cut(s)	852, 1102
TaaI	ACNGT	2 cut(s)	1064, 1494
TaiI	ACGT	3 cut(s)	329, 600, 704
TaqI	TCGA	3 cut(s)	11, 1094, 1700
TaqII	GACCGA	2 cut(s)	38, 167
TatI	WGTACW	2 cut(s)	513, 1525
TauI	GCSGC	7 cut(s)	209, 485, 538, 611, 802, 946, 1022
Tru1I	TTAA	8 cut(s)	974, 1236, 1314, 1487, 1932, 2160, 2372, 2405
Tru9I	TTAA	8 cut(s)	974, 1236, 1314, 1487, 1932, 2160, 2372, 2405
TscAI	CASTG	5 cut(s)	452, 644, 1069, 1468, 1672
TseI	GCWGC	7 cut(s)	247, 427, 430, 1184, 1307, 1439, 2192
TspDTI	ATGAA	7 cut(s)	622, 710, 992, 1584, 2190, 2391, 2432
TspGWI	ACGGA	4 cut(s)	429, 1554, 1573, 1826
TspRI	CASTG	5 cut(s)	452, 644, 1069, 1468, 1672
Vha464I	CTTAAG	1 cut(s)	1931
VpaK11BI	GGWCC	2 cut(s)	50, 676
VspI	ATTAAT	1 cut(s)	1314
XapI	RAATTY	6 cut(s)	580, 1240, 1456, 1745, 2162, 2419
XceI	RCATGY	4 cut(s)	1019, 1439, 1687, 1837
XmiI	GTMKAC	2 cut(s)	11, 1334
XspI	CTAG	2 cut(s)	1851, 2043
Zsp2I	ATGCAT	1 cut(s)	2371

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.