Prupe.7G004800_v2.0.a1

Importin-5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000344
Physical Location & Seq
Forward (+)
541991 .. 553428
11438 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G004800.4

Sequence Viewer

Length: 3354 bp
ATGGCCACCGATCCTACCCAGCAGCAGCAGCAGCAGCAACTGGCCATAATTCTGGGCCCCGACCCGACCCACTTCGAAACCCTAATATCTCATCTTATGTCCTCCAGCAACGACCAACGATCCCAGGCCGAAGCCCTCTTCAACCTCTGCAAGCAGGCCCACCCGGACGCCCTCCTGCTCAAGCTCCTTCACGTCTTGCAGTCCTCGACCCGACCCGAGTCCAGGACCATGGCCGTCATCCTCCTCCGCCGCCAGCTCACCCACGATGACTCCTTCCTCTGGCCCCGCCTCACCCCCAACACCCAGTCCACCCTCAAGTCCCTCCTCCTCTCCTCTCTCCAATCCGAGTCCTCCAAATCCATGTCCAAGAAGCTCTGCGATACCATTTCCGAGCTCGCCTCCTCCGTTCTCCCCGAAAACCAGTGGCCCGAGCTTCTCCCTTTCATGTTCCAGTGCGTCACCTCTGACAATCCCAAACTCCAGGAATCGGCTCTCTTGATTTTCGCCCAATTGGCGCATTACATCGGCGAAACCCTCGTTCCTCACTTGACTACCCTCCACGAGGTCTTCTTCCGGTGCCTGTCTACCTCCGCCAAGAGCGCTGACGTCAGAATCGCTGCGCTCGGGGCGTCAGTGAATTTCATCCAGTGCTTGACTAGTGCGGCCGAGAGGGACAGGTTCCAGGACTTGTTGCCGCTGATGATGCAGACGTTGACCGAGGCGTTGAATTGCGGGCAGGAGGCGACTGCGCAGGAGGCTCTGGAGTTATTGATTGAATTGGCAGGGACTGAACCGAGGTTCTTGAGAAGGCAGTTGGTGGATGTGGTGGGGTCCATGCTTCAGATTGCCGAGGCCGAGTCCTTGGAGGAAGGGACAAGGCATCTGGCCATTGAGTTTGTGATTACCTTGGCCGAGGCCAGGGAGAGGGCACCTGGGATGATGAGGAAGTTGCCGCAGTTCATTCAGAGGTTGTTTGCGATTCTCATGAACATGTTGTTGGACATTGAGGATGAACCTGAGTGGCACGCTGCTGATACAGAGAATGAGGATGCCGGGGAGACAAGCAATTACGGGTTTGGACAGGAGTGTTTGGATAGGCTCTCTATTTCCCTGGGTGGGAATACCATTGTCCCAGTTGCTTCAGAGGTTTTCCCGGCTTTCTTGGCTGCTCCCGAGTGGAAAAAGCACCACGCTGCACACATTGCCCTTGCTCAGATTGCCGAGGGTTGCTCCAAGGTAATGATAAAGAACCTTGAACAGGTCGTGTCAATGGTGTTGAATTCATTCCAAGATCCCCATCCGCGTGTGAGATGGGCAGCAATTAATGCAGTTGGGCAATTGTCAACGGATTTAGGCCCAGAGTTACAAGTCCAGTATCACCAGCGAGTGCTGCCTGCTTTGGCTGGAGCCATGGATGACTTCCAAAATCCTCGAGTACAGGCCCATGCTGCTTCAGCAGTACTCAATTTCAGTGAAAATTGCACCCCAGATATTTTGACACCATACCTGGATGGAATTGTGAGCAAGCTTCTTGTACTTCTACAGAATGGGAAGCAGATGGTGCAGGAGGGTGCTTTAACAGCTTTAGCTTCAGTTGCCGATTCGTCACAGGAGCAATTCCAGAAGTATTATGATGCTGTAATGCCTTACTTGAAAGCTATCTTGATTAATGCTAATGACAAATCCAATCGCATGCTACGTGCCAAATCCATGGAGTGTATTAGTTTGGTTGGAATGGCTGTTGGAAAGGATAAGTTCAGGGATGACGCTAAGCAGATTATGGAAGTCTTGATGTCATTGCAAGGATCTCAAATGGAGGCAGACGATCCTACAACAAGTTACATGTTACAAGCATGGGCCAGACTTTGCAAATGCCTTGGACAGGATTTTCTCCCTTATATGAATGTTGTCATGCCCTCTTTGCTTCAGTCTGCTCAACTGAAGCCTGATGTGACAATTACATCTGCAGATTCAGATGCAGATATTGATGAAGATGATGATAGCATTGAAATGATTACTGTTGGTGATAAAAGGATAGGAATTAGGACTAGTGTCCTGGAGGAAAAAGCTACGGCTTGTAACATGCTATGTTGCTATGCTGATGAATTGAAGGAAGGGTTCTTCCCATGGATTGATCAGGTTGCTCCAACATTGGTTCCTCTTCTCAAATTTTATTTCCATGAAGAAGTTAGAAAGGCAGCTGTTTCAGCGATGCCTGAGCTGTTGCGTTCAGCCAAATTAGCTGTAGAGAAGGGACTTGCACAAGGTCATAATGAGTCCTATATCAAGCAGTTGTCTGATTACATAATACCAGCATTGGTTGAAGCACTACACAAGGAGCCTGAGGTAGAAATTTGTGCAAGCATTTTAGATGCGTTAAAGGAATGTGTGCAGATTTCTGGAGCACTTCTTGATGAAAACCAAGTTAGGTGCATCGTGGATGAAATCAAACAAGTGGTTACAGCCAGTTCATCAAGAAAACAAGAGCGAGCAGAAAGGGCCAAGGCAGAGGACTTCGATGCAGAAGAAGGAGAACTGCTTAAGGAAGAAAATGAACAAGAAGAAGAACTTTTTGATCTGGTCGGTGATTGCTTGGGATCTTTGATTAAGACATTCAAGGCATCTTTCATACCTTTCTTTGATGAGCTTTCAACTTACGTAACACTTATGCTGGGTAAGGATAAAACTGCGGAAGAGAGGCGGATTGCCATTTGTATATTTGATGATATGGCAGAGCATTGCCGTGAAGCAGCTCTTAAATACTATGATACCTACGTTCCTTTCCTATTGGAAGCTTGCAATGATGAAAGTTCAGATGTTCGTCAGGCTGCAGTTTATGGGGTTGGTCTTTGCGCCGAGTATGGTGGATCTGTGTTTAAACCGCTTGTTGGAGAAGCTCTTTCTAGGTTGGATATGGTAATCAGGCACCCTAATGCACAACATTCTGACAATGTGATGGCATATGACAATGCTGTTTCAGCTTTTGGGAAAATTTGCCAGTTCCATCGTGACAGTATAGATGCAACACAGCTCGTCCCAGCCTGGTTAAGCTGTTTACCTATAAAAGGTGATTTGATTGAGGCCAAGGTTGTCCATGACCAGCTATGTTCAATGGTTGAAAGGTCAGATAGGGAGCTTTTAGGGCCCAATAATCAATATCTTCCCAAAATAGTTGCGGTTTTTGCTGAGGTTTTATGCGCTGGTAAAGATTTGGCAACAGAGCAAACTGCTAGTCGTATGGTTAATCTATTAAGGCAGCTTCAACAAACTTTACCGCCCTCTACCCTAGAATCAACCTGGTCATCCTTGCAGCCCCAGCAACAGCTTGCATTGCAATTTATACTCTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEAT HEAT_2 HEAT_EZ IBN_N

Protein Analysis

1118

Amino Acids

124.08

Weight (kDa)

4.81

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000344)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19820
fragaria_vesca FvH4_1g21410 FvH4_1g28300 FvH4_1g28300 FvH4_2g17651 FvH4_3g45470 FvH4_3g45470
malus_domestica MD00G1158900.v1.1 MD03G1004600.v1.1 MD03G1004700.v1.1 MD05G1225200.v1.1 MD11G1005300.v1.1 MD11G1005800.v1.1
prunus_persica Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.6G004500_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1 Prupe.7G004800_v2.0.a1
pyrus_communis pycom03g00480 pycom03g00490 pycom03g00500 pycom11g00440 pycom11g00450
rosa_chinensis RchiOBHm_Chr1g0324711 RchiOBHm_Chr2g0142151 RchiOBHm_Chr2g0142161 RchiOBHm_Chr3g0475691 RchiOBHm_Chr3g0496831 RchiOBHm_Chr4g0399761 RchiOBHm_Chr4g0399771 RchiOBHm_Chr4g0399781 RchiOBHm_Chr4g0419561 RchiOBHm_Chr5g0054221 RchiOBHm_Chr5g0078241 RchiOBHm_Chr5g0083261 RchiOBHm_Chr6g0253081 RchiOBHm_Chr6g0253091 RchiOBHm_Chr6g0253101
rosa_laevigata RLG00000006894 RLG00000017613 RLG00000022498 RLG00000023521 RLG00000029311 RLG00000034357 RLG00000037024
rosa_multiflora Rmu_co8031396.1_g000001 Rmu_co8481369.1_g000001 Rmu_sc0000455.1_g000002 Rmu_sc0000810.1_g000007 Rmu_sc0001438.1_g000001 Rmu_sc0001692.1_g000008 Rmu_sc0001692.1_g000009 Rmu_sc0002130.1_g000004 Rmu_sc0002481.1_g000043 Rmu_sc0004932.1_g000025 Rmu_sc0004967.1_g000012 Rmu_sc0006889.1_g000031 Rmu_sc0011792.1_g000006 Rmu_sc0013655.1_g000001 Rmu_sc0036178.1_g000001 Rmu_sc0040082.1_g000001 Rmu_sc0041125.1_g000001
rosa_roxburghii Rroxscaffold_2G00138150 Rroxscaffold_4G00314380 Rroxscaffold_5G00362400 Rroxscaffold_6G00388660 Rroxscaffold_6G00393970
rosa_rugosa Rorug02G0091400 Rorug03G0288200 Rorug03G0288200 Rorug05G0483800
rosa_samantha Rh1AG440500 Rh2DG505100 Rh2DG505200 Rh3AG331800 Rh3BG368400 Rh3CG343100 Rh3CG364700 Rh3DG367800 Rh4BG222300 Rh4CG233300 Rh5AG533200 Rh5BG560500 Rh5CG583200 Rh5DG381000 Rh5DG569800 Rh6AG307400 Rh6AG307500 Rh6AG307600 Rh7AG013900 Rh7AG014000 Rh7AG153300 Rh7AG154100 Rh7AG283000 Rh7AG385300 Rh7AG446100 Rh7AG487800 Rh7CG302200 Rh7DG365100 Rh7DG365200
rosa_wichuraiana Rw1G008740 Rw2G039350 Rw3G029040 Rw4G018940 Rw4G018950 Rw5G049970 Rw6G002460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 609
Acc16I TGCGCA 1 cut(s) 750
AccB1I GGYRCC 3 cut(s) 576, 928, 2925
AccI GTMKAC 1 cut(s) 584
AccII CGCG 1 cut(s) 1303
AclWI GGATC 7 cut(s) 5, 114, 1286, 1813, 1820, 2605, 2875
AcoI YGGCCR 6 cut(s) 3, 42, 231, 663, 885, 909
AcsI RAATTY 5 cut(s) 637, 1279, 2168, 2352, 2991
AcuI CTGAAG 6 cut(s) 824, 1125, 1437, 1575, 1910, 1961
AcyI GRCGYC 3 cut(s) 168, 606, 629
AfaI GTAC 3 cut(s) 1437, 1461, 1536
AfeI AGCGCT 1 cut(s) 601
AflII CTTAAG 1 cut(s) 2540
AflIII ACRYGT 2 cut(s) 990, 1842
AhlI ACTAGT 2 cut(s) 656, 2048
AjiI CACGTC 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
Alw21I GWGCWC 2 cut(s) 396, 2407
Alw26I GTCTC 1 cut(s) 1052
AlwI GGATC 7 cut(s) 5, 114, 1286, 1813, 1820, 2605, 2875
AlwNI CAGNNNCTG 2 cut(s) 40, 788
Ama87I CYCGRG 5 cut(s) 215, 428, 623, 1172, 1431
Aor51HI AGCGCT 1 cut(s) 601
ApaI GGGCCC 2 cut(s) 59, 3147
ApoI RAATTY 5 cut(s) 637, 1279, 2168, 2352, 2991
ArsI GACNNNNNNTTYG 2 cut(s) 3217, 3249
AseI ATTAAT 2 cut(s) 1323, 1668
Asp700I GAANNNNTTC 1 cut(s) 1283
AspLEI GCGC 6 cut(s) 517, 602, 622, 751, 2855, 3200
AsuC2I CCSGG 3 cut(s) 164, 1054, 1154
AsuHPI GGTGA 7 cut(s) 250, 283, 451, 1370, 2036, 2597, 3080
AsuII TTCGAA 1 cut(s) 75
AvaI CYCGRG 5 cut(s) 215, 428, 623, 1172, 1431
AvaII GGWCC 2 cut(s) 225, 831
AxyI CCTNAGG 1 cut(s) 2343
BaeGI GKGCMC 3 cut(s) 59, 931, 3147
BaeI ACNNNNGTAYC 2 cut(s) 2760, 2793
BalI TGGCCA 3 cut(s) 5, 44, 887
BanI GGYRCC 3 cut(s) 576, 928, 2925
BanII GRGCYC 3 cut(s) 59, 396, 3147
BauI CACGAG 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 559
Bbv12I GWGCWC 2 cut(s) 396, 2407
BbvCI CCTCAGC 1 cut(s) 3186
BccI CCATC 6 cut(s) 1305, 1305, 1505, 1552, 2950, 3012
BceAI ACGGC 3 cut(s) 218, 2088, 2727
BclI TGATCA 1 cut(s) 2134
BcnI CCSGG 3 cut(s) 164, 1054, 1154
BcoDI GTCTC 1 cut(s) 1052
BcuI ACTAGT 2 cut(s) 656, 2048
BfaI CTAG 5 cut(s) 657, 2049, 2904, 3231, 3287
BfmI CTRYAG 4 cut(s) 1541, 1965, 2244, 2829
BfoI RGCGCY 1 cut(s) 603
BfrI CTTAAG 1 cut(s) 2540
BglI GCCNNNNNGGC 1 cut(s) 512
BlpI GCTNAGC 1 cut(s) 1770
BmcAI AGTACT 1 cut(s) 1461
Bme18I GGWCC 2 cut(s) 225, 831
BmeT110I CYCGRG 5 cut(s) 215, 428, 623, 1172, 1431
BmgBI CACGTC 1 cut(s) 193
BmrI ACTGGG 2 cut(s) 298, 1127
BmuI ACTGGG 2 cut(s) 298, 1127
BoxI GACNNNNGTC 2 cut(s) 217, 2051
BpiI GAAGAC 1 cut(s) 559
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 1214, 1246
BpmI CTGGAG 6 cut(s) 88, 464, 782, 1425, 2078, 2421
Bpu10I CCTNAGC 2 cut(s) 2217, 3186
Bpu1102I GCTNAGC 1 cut(s) 1770
Bpu14I TTCGAA 1 cut(s) 75
BpuEI CTTGAG 3 cut(s) 164, 299, 823
BpuMI CCSGG 3 cut(s) 164, 1054, 1154
BsaAI YACGTR 2 cut(s) 1700, 2659
BsaBI GATNNNNATC 1 cut(s) 1296
BsaHI GRCGYC 3 cut(s) 168, 606, 629
BsaWI WCCGGW 1 cut(s) 573
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bse1I ACTGG 9 cut(s) 45, 304, 421, 451, 646, 1133, 1372, 2466, 2998
Bse21I CCTNAGG 1 cut(s) 2343
Bse3DI GCAATG 5 cut(s) 1200, 1796, 2737, 2806, 3329
Bse8I GATNNNNATC 1 cut(s) 1296
BseJI GATNNNNATC 1 cut(s) 1296
BseMI GCAATG 5 cut(s) 1200, 1796, 2737, 2806, 3329
BseMII CTCAG 5 cut(s) 1008, 1226, 2208, 2334, 3177
BseNI ACTGG 9 cut(s) 45, 304, 421, 451, 646, 1133, 1372, 2466, 2998
BseRI GAGGAG 5 cut(s) 233, 314, 317, 322, 391
BseSI GKGCMC 3 cut(s) 59, 931, 3147
BseX3I CGGCCG 1 cut(s) 663
BseYI CCCAGC 4 cut(s) 18, 2671, 3037, 3315
BsgI GTGCAG 3 cut(s) 1179, 1583, 2411
Bsh1236I CGCG 1 cut(s) 1303
Bsh1285I CGRYCG 1 cut(s) 666
BshNI GGYRCC 3 cut(s) 576, 928, 2925
BsiEI CGRYCG 1 cut(s) 666
BsiHKAI GWGCWC 2 cut(s) 396, 2407
BsiHKCI CYCGRG 5 cut(s) 215, 428, 623, 1172, 1431
BsiSI CCGG 4 cut(s) 164, 574, 1053, 1154
BslFI GGGAC 7 cut(s) 304, 686, 799, 886, 1115, 2268, 3020
BsmAI GTCTC 1 cut(s) 1052
BsmFI GGGAC 7 cut(s) 304, 686, 799, 886, 1115, 2268, 3020
BsoBI CYCGRG 5 cut(s) 215, 428, 623, 1172, 1431
Bsp119I TTCGAA 1 cut(s) 75
Bsp120I GGGCCC 2 cut(s) 55, 3143
Bsp1286I GDGCHC 5 cut(s) 59, 396, 931, 2407, 3147
Bsp143I GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
Bsp1720I GCTNAGC 1 cut(s) 1770
Bsp19I CCATGG 4 cut(s) 228, 1410, 1710, 2126
BspCNI CTCAG 5 cut(s) 1009, 1225, 2209, 2335, 3178
BspFNI CGCG 1 cut(s) 1303
BspHI TCATGA 1 cut(s) 984
BspMAI CTGCAG 2 cut(s) 1969, 2833
BspPI GGATC 7 cut(s) 5, 114, 1286, 1813, 1820, 2605, 2875
BspT104I TTCGAA 1 cut(s) 75
BspT107I GGYRCC 3 cut(s) 576, 928, 2925
BspTI CTTAAG 1 cut(s) 2540
BsrDI GCAATG 5 cut(s) 1200, 1796, 2737, 2806, 3329
BsrI ACTGG 9 cut(s) 45, 304, 421, 451, 646, 1133, 1372, 2466, 2998
BssMI GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
BssNI GRCGYC 3 cut(s) 168, 606, 629
BssSI CACGAG 1 cut(s) 560
Bst2BI CACGAG 1 cut(s) 560
Bst4CI ACNGT 2 cut(s) 2020, 3014
Bst6I CTCTTC 3 cut(s) 143, 2166, 2688
BstACI GRCGYC 3 cut(s) 168, 606, 629
BstAFI CTTAAG 1 cut(s) 2540
BstAPI GCANNNNNTGC 3 cut(s) 1202, 1325, 1561
BstBAI YACGTR 2 cut(s) 1700, 2659
BstBI TTCGAA 1 cut(s) 75
BstDEI CTNAG 6 cut(s) 1017, 1212, 1770, 2217, 2343, 3186
BstDSI CCRYGG 4 cut(s) 228, 1410, 1710, 2126
BstENI CCTNNNNNAGG 4 cut(s) 560, 1256, 1880, 3063
BstFNI CGCG 1 cut(s) 1303
BstH2I RGCGCY 1 cut(s) 603
BstHHI GCGC 6 cut(s) 517, 602, 622, 751, 2855, 3200
BstKTI GATC 9 cut(s) 13, 122, 1294, 1808, 1828, 2137, 2578, 2600, 2870
BstMAI GTCTC 1 cut(s) 1052
BstMBI GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
BstMCI CGRYCG 1 cut(s) 666
BstNSI RCATGY 4 cut(s) 994, 1696, 1846, 2086
BstPAI GACNNNNGTC 2 cut(s) 217, 2051
BstSFI CTRYAG 4 cut(s) 1541, 1965, 2244, 2829
BstSLI GKGCMC 3 cut(s) 59, 931, 3147
BstSNI TACGTA 1 cut(s) 2659
BstUI CGCG 1 cut(s) 1303
BstV2I GAAGAC 1 cut(s) 559
BstX2I RGATCY 4 cut(s) 1291, 1805, 2597, 2867
BstXI CCANNNNNNTGG 3 cut(s) 52, 229, 1711
BstYI RGATCY 4 cut(s) 1291, 1805, 2597, 2867
BstZI CGGCCG 1 cut(s) 663
Bsu36I CCTNAGG 1 cut(s) 2343
BtgI CCRYGG 4 cut(s) 228, 1410, 1710, 2126
BtgZI GCGATG 1 cut(s) 2225
BtrI CACGTC 1 cut(s) 193
BtsIMutI CAGTG 5 cut(s) 428, 458, 639, 653, 1477
CaiI CAGNNNCTG 2 cut(s) 40, 788
CciI TCATGA 1 cut(s) 984
CfoI GCGC 6 cut(s) 517, 602, 622, 751, 2855, 3200
CseI GACGC 4 cut(s) 176, 445, 618, 1775
CsiI ACCWGGT 1 cut(s) 3296
Csp6I GTAC 3 cut(s) 1436, 1460, 1535
CviQI GTAC 3 cut(s) 1436, 1460, 1535
DdeI CTNAG 6 cut(s) 1017, 1212, 1770, 2217, 2343, 3186
DpnI GATC 9 cut(s) 12, 121, 1293, 1807, 1827, 2136, 2577, 2599, 2869
DpnII GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
DraI TTTAAA 1 cut(s) 2878
EaeI YGGCCR 6 cut(s) 3, 42, 231, 663, 885, 909
EagI CGGCCG 1 cut(s) 663
Eam1104I CTCTTC 3 cut(s) 143, 2166, 2688
EarI CTCTTC 3 cut(s) 143, 2166, 2688
EciI GGCGGA 3 cut(s) 236, 580, 2716
Ecl136II GAGCTC 1 cut(s) 394
EclXI CGGCCG 1 cut(s) 663
Eco105I TACGTA 1 cut(s) 2659
Eco24I GRGCYC 3 cut(s) 59, 396, 3147
Eco47I GGWCC 2 cut(s) 225, 831
Eco47III AGCGCT 1 cut(s) 601
Eco52I CGGCCG 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 6 cut(s) 824, 1125, 1437, 1575, 1910, 1961
Eco81I CCTNAGG 1 cut(s) 2343
Eco88I CYCGRG 5 cut(s) 215, 428, 623, 1172, 1431
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 4 cut(s) 560, 1256, 1880, 3063
EcoO109I RGGNCCY 2 cut(s) 56, 3143
EcoRI GAATTC 1 cut(s) 1279
EcoT38I GRGCYC 3 cut(s) 59, 396, 3147
FalI AAGNNNNNCTT 4 cut(s) 2553, 2585, 2739, 2771
FaqI GGGAC 7 cut(s) 304, 686, 799, 886, 1115, 2268, 3020
FauI CCCGC 2 cut(s) 293, 725
FauNDI CATATG 1 cut(s) 2962
FbaI TGATCA 1 cut(s) 2134
FblI GTMKAC 1 cut(s) 584
FriOI GRGCYC 3 cut(s) 59, 396, 3147
FspBI CTAG 5 cut(s) 657, 2049, 2904, 3231, 3287
FspI TGCGCA 1 cut(s) 750
GlaI GCGC 6 cut(s) 516, 601, 621, 750, 2854, 3199
GsaI CCCAGC 4 cut(s) 22, 2675, 3041, 3319
GsuI CTGGAG 6 cut(s) 88, 464, 782, 1425, 2078, 2421
HaeII RGCGCY 1 cut(s) 603
HapII CCGG 4 cut(s) 164, 574, 1053, 1154
HgaI GACGC 4 cut(s) 176, 445, 618, 1775
HhaI GCGC 6 cut(s) 517, 602, 622, 751, 2855, 3200
Hin1I GRCGYC 3 cut(s) 168, 606, 629
Hin6I GCGC 6 cut(s) 515, 600, 620, 749, 2853, 3198
HinP1I GCGC 6 cut(s) 515, 600, 620, 749, 2853, 3198
HincII GTYRAC 2 cut(s) 714, 1344
HindII GTYRAC 2 cut(s) 714, 1344
HindIII AAGCTT 2 cut(s) 1526, 2793
HpaII CCGG 4 cut(s) 164, 574, 1053, 1154
HphI GGTGA 7 cut(s) 250, 283, 451, 1370, 2036, 2597, 3080
Hpy166II GTNNAC 5 cut(s) 309, 585, 714, 1344, 3056
Hpy8I GTNNAC 5 cut(s) 309, 585, 714, 1344, 3056
HpyAV CCTTC 8 cut(s) 197, 283, 801, 863, 2104, 2108, 2245, 2522
HpyCH4III ACNGT 2 cut(s) 2020, 3014
HpyCH4IV ACGT 6 cut(s) 192, 606, 710, 1699, 2658, 2775
HpyF3I CTNAG 6 cut(s) 1017, 1212, 1770, 2217, 2343, 3186
HpySE526I ACGT 6 cut(s) 192, 606, 710, 1699, 2658, 2775
Hsp92I GRCGYC 3 cut(s) 168, 606, 629
HspAI GCGC 6 cut(s) 515, 600, 620, 749, 2853, 3198
Ksp22I TGATCA 1 cut(s) 2134
Kzo9I GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
LmnI GCTCC 9 cut(s) 189, 1174, 1235, 1406, 1612, 2149, 2338, 2402, 3133
MabI ACCWGGT 1 cut(s) 3296
MaeI CTAG 5 cut(s) 657, 2049, 2904, 3231, 3287
MaeII ACGT 6 cut(s) 192, 606, 710, 1699, 2658, 2775
MalI GATC 9 cut(s) 12, 121, 1293, 1807, 1827, 2136, 2577, 2599, 2869
MboI GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
MfeI CAATTG 2 cut(s) 509, 1337
MflI RGATCY 4 cut(s) 1291, 1805, 2597, 2867
MhlI GDGCHC 5 cut(s) 59, 396, 931, 2407, 3147
MlsI TGGCCA 3 cut(s) 5, 44, 887
MluNI TGGCCA 3 cut(s) 5, 44, 887
MlyI GAGTC 5 cut(s) 227, 263, 356, 866, 2285
MmeI TCCRAC 6 cut(s) 978, 1711, 1723, 2171, 2869, 2889
Mox20I TGGCCA 3 cut(s) 5, 44, 887
MroXI GAANNNNTTC 1 cut(s) 1283
MscI TGGCCA 3 cut(s) 5, 44, 887
MslI CAYNNNNRTG 5 cut(s) 989, 1302, 2009, 2742, 2931
Msp20I TGGCCA 3 cut(s) 5, 44, 887
MspA1I CMGCKG 2 cut(s) 697, 2201
MspCI CTTAAG 1 cut(s) 2540
MspI CCGG 4 cut(s) 164, 574, 1053, 1154
MssI GTTTAAAC 1 cut(s) 2878
MunI CAATTG 2 cut(s) 509, 1337
MvnI CGCG 1 cut(s) 1303
NciI CCSGG 3 cut(s) 164, 1054, 1154
NcoI CCATGG 4 cut(s) 228, 1410, 1710, 2126
NdeI CATATG 1 cut(s) 2962
NdeII GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
NmeAIII GCCGAG 6 cut(s) 691, 874, 880, 937, 1246, 2881
NmuCI GTSAC 4 cut(s) 457, 1605, 1951, 3008
NsbI TGCGCA 1 cut(s) 750
NspI RCATGY 4 cut(s) 994, 1696, 1846, 2086
NspV TTCGAA 1 cut(s) 75
PaeI GCATGC 1 cut(s) 1696
PaeR7I CTCGAG 1 cut(s) 1431
PagI TCATGA 1 cut(s) 984
PasI CCCWGGG 1 cut(s) 1111
PciI ACATGT 2 cut(s) 990, 1842
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 1696
PdmI GAANNNNTTC 1 cut(s) 1283
PfeI GAWTC 6 cut(s) 485, 612, 979, 1601, 1970, 3290
PfoI TCCNGGA 4 cut(s) 221, 480, 681, 2055
PleI GAGTC 5 cut(s) 226, 263, 355, 865, 2284
PmeI GTTTAAAC 1 cut(s) 2878
PpsI GAGTC 5 cut(s) 226, 263, 355, 865, 2284
Ppu21I YACGTR 2 cut(s) 1700, 2659
PscI ACATGT 2 cut(s) 990, 1842
PshAI GACNNNNGTC 2 cut(s) 217, 2051
PshBI ATTAAT 2 cut(s) 1323, 1668
Psp124BI GAGCTC 1 cut(s) 396
PspFI CCCAGC 4 cut(s) 18, 2671, 3037, 3315
PspOMI GGGCCC 2 cut(s) 55, 3143
PspXI VCTCGAGB 1 cut(s) 1431
PstI CTGCAG 2 cut(s) 1969, 2833
PstNI CAGNNNCTG 2 cut(s) 40, 788
PsuI RGATCY 4 cut(s) 1291, 1805, 2597, 2867
PvuII CAGCTG 1 cut(s) 2201
RsaI GTAC 3 cut(s) 1437, 1461, 1536
RsaNI GTAC 3 cut(s) 1436, 1460, 1535
RseI CAYNNNNRTG 5 cut(s) 989, 1302, 2009, 2742, 2931
SacI GAGCTC 1 cut(s) 396
Sau3AI GATC 9 cut(s) 10, 119, 1291, 1805, 1825, 2134, 2575, 2597, 2867
ScaI AGTACT 1 cut(s) 1461
SchI GAGTC 5 cut(s) 227, 263, 356, 866, 2285
SduI GDGCHC 5 cut(s) 59, 396, 931, 2407, 3147
SexAI ACCWGGT 1 cut(s) 3296
SfcI CTRYAG 4 cut(s) 1541, 1965, 2244, 2829
Sfr274I CTCGAG 1 cut(s) 1431
SfuI TTCGAA 1 cut(s) 75
SinI GGWCC 2 cut(s) 225, 831
SlaI CTCGAG 1 cut(s) 1431
SmiMI CAYNNNNRTG 5 cut(s) 989, 1302, 2009, 2742, 2931
SmlI CTYRAG 5 cut(s) 179, 314, 802, 1431, 2540
SmoI CTYRAG 5 cut(s) 179, 314, 802, 1431, 2540
SnaBI TACGTA 1 cut(s) 2659
SpeI ACTAGT 2 cut(s) 656, 2048
SphI GCATGC 1 cut(s) 1696
SspMI CTAG 5 cut(s) 657, 2049, 2904, 3231, 3287
SstI GAGCTC 1 cut(s) 396
TaaI ACNGT 2 cut(s) 2020, 3014
TaiI ACGT 6 cut(s) 195, 609, 713, 1702, 2661, 2778
TaqI TCGA 4 cut(s) 75, 206, 1432, 2517
TaqII GACCGA 1 cut(s) 731
TatI WGTACW 3 cut(s) 1435, 1459, 1534
TauI GCSGC 4 cut(s) 252, 665, 697, 955
TfiI GAWTC 6 cut(s) 485, 612, 979, 1601, 1970, 3290
TscAI CASTG 5 cut(s) 428, 458, 639, 653, 1477
TseFI GTSAC 4 cut(s) 457, 1605, 1951, 3008
Tsp45I GTSAC 4 cut(s) 457, 1605, 1951, 3008
TspGWI ACGGA 2 cut(s) 394, 1361
TspRI CASTG 5 cut(s) 428, 458, 639, 653, 1477
Vha464I CTTAAG 1 cut(s) 2540
VpaK11BI GGWCC 2 cut(s) 225, 831
VspI ATTAAT 2 cut(s) 1323, 1668
XagI CCTNNNNNAGG 4 cut(s) 560, 1256, 1880, 3063
XapI RAATTY 5 cut(s) 637, 1279, 2168, 2352, 2991
XceI RCATGY 4 cut(s) 994, 1696, 1846, 2086
XhoI CTCGAG 1 cut(s) 1431
XmiI GTMKAC 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 1283
XspI CTAG 5 cut(s) 657, 2049, 2904, 3231, 3287
ZraI GACGTC 1 cut(s) 607
ZrmI AGTACT 1 cut(s) 1461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.