Importin-5-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 506 |
| AccII | CGCG | 3 cut(s) | 30, 399, 598 |
| AciI | CCGC | 5 cut(s) | 5, 36, 127, 620, 647 |
| AclWI | GGATC | 4 cut(s) | 35, 177, 423, 693 |
| AcoI | YGGCCR | 1 cut(s) | 453 |
| AcsI | RAATTY | 2 cut(s) | 316, 571 |
| AcuI | CTGAAG | 1 cut(s) | 366 |
| AdeI | CACNNNGTG | 1 cut(s) | 227 |
| AfaI | GTAC | 1 cut(s) | 131 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 91, 301 |
| AgsI | TTSAA | 1 cut(s) | 464 |
| AjnI | CCWGG | 1 cut(s) | 76 |
| AluBI | AGCT | 5 cut(s) | 238, 358, 365, 451, 718 |
| AluI | AGCT | 5 cut(s) | 238, 358, 365, 451, 718 |
| Alw21I | GWGCWC | 2 cut(s) | 62, 218 |
| Alw26I | GTCTC | 1 cut(s) | 437 |
| AlwI | GGATC | 4 cut(s) | 35, 177, 423, 693 |
| Ama87I | CYCGRG | 1 cut(s) | 44 |
| AoxI | GGCC | 3 cut(s) | 64, 70, 453 |
| ApeKI | GCWGC | 7 cut(s) | 25, 81, 355, 512, 550, 553, 715 |
| ApoI | RAATTY | 2 cut(s) | 316, 571 |
| AspLEI | GCGC | 2 cut(s) | 30, 715 |
| AsuC2I | CCSGG | 2 cut(s) | 45, 46 |
| AsuHPI | GGTGA | 2 cut(s) | 50, 626 |
| AvaI | CYCGRG | 1 cut(s) | 44 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 384, 417 |
| BalI | TGGCCA | 1 cut(s) | 455 |
| BanII | GRGCYC | 1 cut(s) | 150 |
| Bbv12I | GWGCWC | 2 cut(s) | 62, 218 |
| BbvI | GCAGC | 7 cut(s) | 37, 68, 367, 499, 562, 565, 727 |
| BccI | CCATC | 3 cut(s) | 317, 321, 385 |
| BceAI | ACGGC | 1 cut(s) | 51 |
| BciT130I | CCWGG | 1 cut(s) | 78 |
| BcnI | CCSGG | 2 cut(s) | 45, 46 |
| BcoDI | GTCTC | 1 cut(s) | 437 |
| BfaI | CTAG | 2 cut(s) | 476, 560 |
| BfmI | CTRYAG | 1 cut(s) | 513 |
| BfuAI | ACCTGC | 1 cut(s) | 506 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 10, 78 |
| BisI | GCNGC | 8 cut(s) | 5, 26, 82, 356, 513, 551, 554, 716 |
| BlsI | GCNGC | 8 cut(s) | 6, 27, 83, 357, 514, 552, 555, 717 |
| Bme1390I | CCNGG | 3 cut(s) | 45, 46, 78 |
| BmeT110I | CYCGRG | 1 cut(s) | 44 |
| BmiI | GGNNCC | 2 cut(s) | 254, 497 |
| BmrFI | CCNGG | 3 cut(s) | 45, 46, 78 |
| BmsI | GCATC | 1 cut(s) | 278 |
| Bpu10I | CCTNAGC | 2 cut(s) | 61, 300 |
| BpuMI | CCSGG | 2 cut(s) | 45, 46 |
| BsaJI | CCNNGG | 5 cut(s) | 8, 44, 45, 67, 90 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 91, 301 |
| BseBI | CCWGG | 1 cut(s) | 78 |
| BseDI | CCNNGG | 5 cut(s) | 8, 44, 45, 67, 90 |
| BseGI | GGATG | 3 cut(s) | 269, 284, 332 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 91, 301 |
| BseMII | CTCAG | 3 cut(s) | 75, 314, 461 |
| BseXI | GCAGC | 7 cut(s) | 37, 68, 367, 499, 562, 565, 727 |
| BseYI | CCCAGC | 2 cut(s) | 365, 718 |
| Bsh1236I | CGCG | 3 cut(s) | 30, 399, 598 |
| BshFI | GGCC | 3 cut(s) | 66, 72, 455 |
| BsiHKAI | GWGCWC | 2 cut(s) | 62, 218 |
| BsiHKCI | CYCGRG | 1 cut(s) | 44 |
| BsiSI | CCGG | 1 cut(s) | 45 |
| BslFI | GGGAC | 1 cut(s) | 63 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 91, 301 |
| BsmAI | GTCTC | 1 cut(s) | 437 |
| BsmFI | GGGAC | 1 cut(s) | 63 |
| BsnI | GGCC | 3 cut(s) | 66, 72, 455 |
| BsoBI | CYCGRG | 1 cut(s) | 44 |
| Bsp1286I | GDGCHC | 3 cut(s) | 62, 150, 218 |
| Bsp143I | GATC | 4 cut(s) | 40, 169, 415, 685 |
| BspACI | CCGC | 5 cut(s) | 5, 36, 127, 620, 647 |
| BspANI | GGCC | 3 cut(s) | 66, 72, 455 |
| BspCNI | CTCAG | 3 cut(s) | 74, 313, 460 |
| BspFNI | CGCG | 3 cut(s) | 30, 399, 598 |
| BspLI | GGNNCC | 2 cut(s) | 254, 497 |
| BspMAI | CTGCAG | 1 cut(s) | 517 |
| BspMI | ACCTGC | 1 cut(s) | 506 |
| BspPI | GGATC | 4 cut(s) | 35, 177, 423, 693 |
| BssECI | CCNNGG | 5 cut(s) | 8, 44, 45, 67, 90 |
| BssMI | GATC | 4 cut(s) | 40, 169, 415, 685 |
| BssT1I | CCWWGG | 1 cut(s) | 8 |
| Bst2UI | CCWGG | 1 cut(s) | 78 |
| Bst4CI | ACNGT | 1 cut(s) | 110 |
| Bst6I | CTCTTC | 1 cut(s) | 645 |
| BstC8I | GCNNGC | 3 cut(s) | 74, 453, 596 |
| BstDEI | CTNAG | 3 cut(s) | 61, 300, 447 |
| BstDSI | CCRYGG | 2 cut(s) | 67, 90 |
| BstEII | GGTNACC | 1 cut(s) | 614 |
| BstF5I | GGATG | 3 cut(s) | 269, 284, 332 |
| BstFNI | CGCG | 3 cut(s) | 30, 399, 598 |
| BstHHI | GCGC | 2 cut(s) | 30, 715 |
| BstKTI | GATC | 4 cut(s) | 43, 172, 418, 688 |
| BstMAI | GTCTC | 1 cut(s) | 437 |
| BstMBI | GATC | 4 cut(s) | 40, 169, 415, 685 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 10, 78, 145, 604, 749 |
| BstNI | CCWGG | 1 cut(s) | 78 |
| BstNSI | RCATGY | 1 cut(s) | 269 |
| BstPI | GGTNACC | 1 cut(s) | 614 |
| BstSCI | CCNGG | 3 cut(s) | 43, 44, 76 |
| BstSFI | CTRYAG | 1 cut(s) | 513 |
| BstUI | CGCG | 3 cut(s) | 30, 399, 598 |
| BstV1I | GCAGC | 7 cut(s) | 37, 68, 367, 499, 562, 565, 727 |
| BstX2I | RGATCY | 1 cut(s) | 169 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 682 |
| BstYI | RGATCY | 1 cut(s) | 169 |
| BsuRI | GGCC | 3 cut(s) | 66, 72, 455 |
| BtgI | CCRYGG | 2 cut(s) | 67, 90 |
| BtsCI | GGATG | 3 cut(s) | 269, 284, 332 |
| BtsI | GCAGTG | 1 cut(s) | 497 |
| BtsIMutI | CAGTG | 2 cut(s) | 390, 497 |
| BveI | ACCTGC | 1 cut(s) | 506 |
| Cac8I | GCNNGC | 3 cut(s) | 74, 453, 596 |
| CfoI | GCGC | 2 cut(s) | 30, 715 |
| Cfr9I | CCCGGG | 1 cut(s) | 44 |
| CseI | GACGC | 2 cut(s) | 19, 596 |
| Csp6I | GTAC | 1 cut(s) | 130 |
| CviAII | CATG | 7 cut(s) | 266, 440, 501, 676, 700, 740, 772 |
| CviQI | GTAC | 1 cut(s) | 130 |
| DdeI | CTNAG | 3 cut(s) | 61, 300, 447 |
| DpnI | GATC | 4 cut(s) | 42, 171, 417, 687 |
| DpnII | GATC | 4 cut(s) | 40, 169, 415, 685 |
| DraIII | CACNNNGTG | 1 cut(s) | 227 |
| EaeI | YGGCCR | 1 cut(s) | 453 |
| Eam1104I | CTCTTC | 1 cut(s) | 645 |
| EarI | CTCTTC | 1 cut(s) | 645 |
| EciI | GGCGGA | 1 cut(s) | 662 |
| Eco130I | CCWWGG | 1 cut(s) | 8 |
| Eco24I | GRGCYC | 1 cut(s) | 150 |
| Eco57I | CTGAAG | 1 cut(s) | 366 |
| Eco88I | CYCGRG | 1 cut(s) | 44 |
| Eco91I | GGTNACC | 1 cut(s) | 614 |
| EcoO65I | GGTNACC | 1 cut(s) | 614 |
| EcoRI | GAATTC | 1 cut(s) | 316 |
| EcoRII | CCWGG | 1 cut(s) | 76 |
| EcoT14I | CCWWGG | 1 cut(s) | 8 |
| EcoT22I | ATGCAT | 1 cut(s) | 271 |
| EcoT38I | GRGCYC | 1 cut(s) | 150 |
| ErhI | CCWWGG | 1 cut(s) | 8 |
| FaeI | CATG | 7 cut(s) | 269, 443, 504, 679, 703, 743, 775 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 156, 188, 175, 207 |
| FaqI | GGGAC | 1 cut(s) | 63 |
| FatI | CATG | 7 cut(s) | 265, 439, 500, 675, 699, 739, 771 |
| FauI | CCCGC | 1 cut(s) | 627 |
| Fnu4HI | GCNGC | 8 cut(s) | 5, 26, 82, 356, 513, 551, 554, 716 |
| FokI | GGATG | 3 cut(s) | 256, 271, 339 |
| FriOI | GRGCYC | 1 cut(s) | 150 |
| Fsp4HI | GCNGC | 8 cut(s) | 5, 26, 82, 356, 513, 551, 554, 716 |
| FspBI | CTAG | 2 cut(s) | 476, 560 |
| GlaI | GCGC | 2 cut(s) | 29, 714 |
| GluI | GCNGC | 8 cut(s) | 5, 26, 82, 356, 513, 551, 554, 716 |
| GsaI | CCCAGC | 2 cut(s) | 369, 722 |
| HaeIII | GGCC | 3 cut(s) | 66, 72, 455 |
| HapII | CCGG | 1 cut(s) | 45 |
| HgaI | GACGC | 2 cut(s) | 19, 596 |
| HhaI | GCGC | 2 cut(s) | 30, 715 |
| Hin1II | CATG | 7 cut(s) | 269, 443, 504, 679, 703, 743, 775 |
| Hin6I | GCGC | 2 cut(s) | 28, 713 |
| HinP1I | GCGC | 2 cut(s) | 28, 713 |
| HindIII | AAGCTT | 1 cut(s) | 236 |
| HinfI | GANTC | 4 cut(s) | 51, 348, 445, 506 |
| HpaII | CCGG | 1 cut(s) | 45 |
| HphI | GGTGA | 2 cut(s) | 50, 626 |
| Hpy188I | TCNGA | 3 cut(s) | 274, 322, 391 |
| Hpy188III | TCNNGA | 2 cut(s) | 232, 413 |
| HpyAV | CCTTC | 1 cut(s) | 266 |
| HpyCH4III | ACNGT | 1 cut(s) | 110 |
| HpyCH4V | TGCA | 5 cut(s) | 269, 355, 439, 515, 775 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 10, 78, 145, 604, 749 |
| HpyF3I | CTNAG | 3 cut(s) | 61, 300, 447 |
| Hsp92II | CATG | 7 cut(s) | 269, 443, 504, 679, 703, 743, 775 |
| HspAI | GCGC | 2 cut(s) | 28, 713 |
| Kzo9I | GATC | 4 cut(s) | 40, 169, 415, 685 |
| LmnI | GCTCC | 1 cut(s) | 501 |
| Lsp1109I | GCAGC | 7 cut(s) | 37, 68, 367, 499, 562, 565, 727 |
| LweI | GCATC | 1 cut(s) | 278 |
| MaeI | CTAG | 2 cut(s) | 476, 560 |
| MaeIII | GTNAC | 2 cut(s) | 95, 614 |
| MalI | GATC | 4 cut(s) | 42, 171, 417, 687 |
| MboI | GATC | 4 cut(s) | 40, 169, 415, 685 |
| MboII | GAAGA | 3 cut(s) | 164, 372, 662 |
| MflI | RGATCY | 1 cut(s) | 169 |
| MhlI | GDGCHC | 3 cut(s) | 62, 150, 218 |
| MlsI | TGGCCA | 1 cut(s) | 455 |
| MluCI | AATT | 3 cut(s) | 204, 316, 571 |
| MluNI | TGGCCA | 1 cut(s) | 455 |
| MlyI | GAGTC | 3 cut(s) | 45, 439, 515 |
| MnlI | CCTC | 2 cut(s) | 646, 741 |
| Mox20I | TGGCCA | 1 cut(s) | 455 |
| Mph1103I | ATGCAT | 1 cut(s) | 271 |
| MscI | TGGCCA | 1 cut(s) | 455 |
| MseI | TTAA | 3 cut(s) | 203, 240, 659 |
| Msp20I | TGGCCA | 1 cut(s) | 455 |
| MspA1I | CMGCKG | 4 cut(s) | 36, 365, 451, 718 |
| MspI | CCGG | 1 cut(s) | 45 |
| MspR9I | CCNGG | 3 cut(s) | 45, 46, 78 |
| MvaI | CCWGG | 1 cut(s) | 78 |
| MvnI | CGCG | 3 cut(s) | 30, 399, 598 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 10, 78, 145, 604, 749 |
| NciI | CCSGG | 2 cut(s) | 45, 46 |
| NdeII | GATC | 4 cut(s) | 40, 169, 415, 685 |
| NlaIII | CATG | 7 cut(s) | 269, 443, 504, 679, 703, 743, 775 |
| NlaIV | GGNNCC | 2 cut(s) | 254, 497 |
| NmuCI | GTSAC | 2 cut(s) | 95, 614 |
| NsiI | ATGCAT | 1 cut(s) | 271 |
| NspI | RCATGY | 1 cut(s) | 269 |
| PfeI | GAWTC | 1 cut(s) | 348 |
| PkrI | GCNGC | 8 cut(s) | 6, 27, 83, 357, 514, 552, 555, 717 |
| PleI | GAGTC | 3 cut(s) | 45, 439, 514 |
| PpsI | GAGTC | 3 cut(s) | 45, 439, 514 |
| Psp6I | CCWGG | 1 cut(s) | 76 |
| PspEI | GGTNACC | 1 cut(s) | 614 |
| PspFI | CCCAGC | 2 cut(s) | 365, 718 |
| PspGI | CCWGG | 1 cut(s) | 76 |
| PspN4I | GGNNCC | 2 cut(s) | 254, 497 |
| PstI | CTGCAG | 1 cut(s) | 517 |
| PsuI | RGATCY | 1 cut(s) | 169 |
| PvuII | CAGCTG | 3 cut(s) | 365, 451, 718 |
| RsaI | GTAC | 1 cut(s) | 131 |
| RsaNI | GTAC | 1 cut(s) | 130 |
| SaqAI | TTAA | 3 cut(s) | 203, 240, 659 |
| SatI | GCNGC | 8 cut(s) | 5, 26, 82, 356, 513, 551, 554, 716 |
| Sau3AI | GATC | 4 cut(s) | 40, 169, 415, 685 |
| SchI | GAGTC | 3 cut(s) | 45, 439, 515 |
| ScrFI | CCNGG | 3 cut(s) | 45, 46, 78 |
| SduI | GDGCHC | 3 cut(s) | 62, 150, 218 |
| SfaNI | GCATC | 1 cut(s) | 278 |
| SfcI | CTRYAG | 1 cut(s) | 513 |
| SmaI | CCCGGG | 1 cut(s) | 46 |
| Sse9I | AATT | 3 cut(s) | 204, 316, 571 |
| SsiI | CCGC | 5 cut(s) | 5, 36, 127, 620, 647 |
| SspMI | CTAG | 2 cut(s) | 476, 560 |
| StyD4I | CCNGG | 3 cut(s) | 43, 44, 76 |
| StyI | CCWWGG | 1 cut(s) | 8 |
| TaaI | ACNGT | 1 cut(s) | 110 |
| TaqI | TCGA | 1 cut(s) | 218 |
| TasI | AATT | 3 cut(s) | 204, 316, 571 |
| TauI | GCSGC | 1 cut(s) | 7 |
| TfiI | GAWTC | 1 cut(s) | 348 |
| Tru1I | TTAA | 3 cut(s) | 203, 240, 659 |
| Tru9I | TTAA | 3 cut(s) | 203, 240, 659 |
| TscAI | CASTG | 2 cut(s) | 390, 497 |
| TseFI | GTSAC | 2 cut(s) | 95, 614 |
| TseI | GCWGC | 7 cut(s) | 25, 81, 355, 512, 550, 553, 715 |
| Tsp45I | GTSAC | 2 cut(s) | 95, 614 |
| TspDTI | ATGAA | 3 cut(s) | 273, 398, 760 |
| TspGWI | ACGGA | 1 cut(s) | 79 |
| TspMI | CCCGGG | 1 cut(s) | 44 |
| TspRI | CASTG | 2 cut(s) | 390, 497 |
| XapI | RAATTY | 2 cut(s) | 316, 571 |
| XceI | RCATGY | 1 cut(s) | 269 |
| XmaI | CCCGGG | 1 cut(s) | 44 |
| XspI | CTAG | 2 cut(s) | 476, 560 |
| Zsp2I | ATGCAT | 1 cut(s) | 271 |