FvH4_2g35000

F-box LRR-repeat protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000358
Physical Location & Seq
Forward (+)
25887469 .. 25889438
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35000.t1

Sequence Viewer

Length: 1080 bp
ATGGCCCAAAGATTTGGGGGAATTTGTGTGCTAGGTGAGGATGAGCTAGACCTAATCGTCAACAAGTTTAGCGACCCAGCTGATCGGAAATCGGTCTCGGAGGTCTGCAAGCTATGGTATAAAGTGGAGAGTCTCAACCGGTCATCACTTCGTCTTCTCGCTCCCGGTTGTCCCCGTCAGGTATTCCCAAGATACCCGAATCTAGTCAAATTCGAAACAACCCGCTCTATCTCAGACTGTGATCTCGCATTTTTGGGTGAAACATGTTCCAAACTTGAGGTCGTCAACCTCAATGGTCTTACTGGGGTTTATCCACCTTTCGTTTTCTCTGGCGTAGGTCTTCTCAGTTTAGCAAAACATTGTCCAAAGTTGTCAAAGGTTATGCTTAATAATGTGAGACTGCATATTATTCATTCTGTACCTATTTCGCTTATAAGTTTAGCACCCAATTTGAGGCATTTGGATTTGGGAAGTTGTCCTGTTGTCACGGACGAAACCCTTGAAGCAATTGGGTCTTCCAGTTCGATTAGCTATTTGAATTTGGCAGGGTGTTTCAAGATTACTGATCGCGGGTTGGGTTTTCTAGCAAATGGGTCTGTCTCAAAATCCCTCAAGACATTGTTCCTTGCTTGGATCAAAGGCCTTACTGATGTTGGGGTCTCGCATTTGCACAAAATGCATTGCTTGGAGCACCTTAGTTTGGCTAACCGTTCGGTATCAGAGATCACCGATATTGGAGGTGTCGCAATCTCGACCATTGCTACCCTCAAAAGATTGAGCTTGCAGTTGCTGCACAAAATATCAGACCTCACTATTAGGGCTCTTGCTCGGAATTGCCCCAACTTGGAATTCCTCGATTTAAGTAAATGTGGAATAACTGGAGCTGGTGTACGTGCATTTTCGGGTCACCCTAGCTTACGGACTATTATTCTGCGCTTCTGTGAAGTCGACGTATCTGATTTAGACCATTTGGTGCTTAGGTGCCCATCATTGAAGTCTATTCTGCTGGACAATAAAAAGCTAGAGCAGATGTTGCCGCTAATAGGGACGCAAACTAGAAGTTTTGTGAAAGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_6

Protein Analysis

360

Amino Acids

39.26

Weight (kDa)

9.02

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box_5 PF18511 18 - 48 5.3e-08 F-box
DUF7885 PF25372 73 - 314 3.5e-14 Leucine Rich Repeat Domain of unknown function (DUF7885)
LRR_At5g56370 PF24758 256 - 340 9.7e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000358)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35670
fragaria_vesca FvH4_1g05120 FvH4_1g05120 FvH4_2g32511 FvH4_2g32520 FvH4_2g32530 FvH4_2g32540 FvH4_2g32590 FvH4_2g32600 FvH4_2g32610 FvH4_2g32630 FvH4_2g34980 FvH4_2g34990 FvH4_2g35000 FvH4_2g35530 FvH4_3g30491 FvH4_5g19260 FvH4_5g19260 FvH4_5g33281 FvH4_6g06400 FvH4_6g07350 FvH4_6g07350 FvH4_6g07350 FvH4_6g10172 FvH4_6g10180 FvH4_6g11570 FvH4_6g11570 FvH4_6g11570 FvH4_6g11570 FvH4_6g11570 FvH4_6g11570 FvH4_6g15310 FvH4_6g41671 FvH4_6g41680 FvH4_6g41680
malus_domestica MD09G1117800.v1.1 MD15G1093400.v1.1 MD17G1109100.v1.1
prunus_persica Prupe.1G448200_v2.0.a1 Prupe.3G206100_v2.0.a1 Prupe.3G206100_v2.0.a1
pyrus_communis pycom09g04310 pycom17g10160
rosa_chinensis RchiOBHm_Chr2g0157391 RchiOBHm_Chr2g0157401 RchiOBHm_Chr3g0468781 RchiOBHm_Chr6g0306251 RchiOBHm_Chr6g0307021 RchiOBHm_Chr6g0307031
rosa_laevigata RLG00000010745 RLG00000010746 RLG00000010811 RLG00000012028 RLG00000020986 RLG00000024410
rosa_multiflora Rmu_co8477457.1_g000001 Rmu_sc0000126.1_g000004 Rmu_sc0000195.1_g000016 Rmu_sc0001639.1_g000026 Rmu_sc0001639.1_g000027 Rmu_sc0001639.1_g000028 Rmu_sc0005634.1_g000011 Rmu_sc0005634.1_g000012 Rmu_sc0017002.1_g000001 Rmu_sc0022075.1_g000001
rosa_roxburghii Rroxscaffold_2G00092220 Rroxscaffold_2G00092230 Rroxscaffold_6G00412570 Rroxscaffold_6G00412580 Rroxscaffold_7G00161250 Rroxscaffold_7G00161270
rosa_rugosa Rorug02G0460900 Rorug02G0461000 Rorug03G0096400 Rorug03G0096400 Rorug06G0348200 Rorug06G0348400 Rorug06G0355000 Rorug06G0355100
rosa_samantha Rh2AG525300 Rh2AG525400 Rh2BG539400 Rh2CG510000 Rh2DG548500 Rh3AG145800 Rh3DG167000 Rh6BG433900 Rh6BG434000 Rh6BG440200 Rh6BG440600 Rh6CG353800 Rh6CG474800 Rh6CG475100 Rh6CG482000 Rh6CG482100 Rh6DG461100 Rh6DG461300 Rh6DG461400 Rh6DG468300 Rh6DG468400
rosa_wichuraiana Rw2G043480 Rw2G043490 Rw3G013740 Rw6G040150 Rw6G040670 Rw6G040680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AasI GACNNNNNNGTC 1 cut(s) 56
AccB1I GGYRCC 1 cut(s) 981
AccBSI CCGCTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 948
AccII CGCG 1 cut(s) 570
AciI CCGC 3 cut(s) 223, 570, 1037
AclWI GGATC 1 cut(s) 641
AcsI RAATTY 4 cut(s) 21, 209, 538, 848
AfaI GTAC 2 cut(s) 420, 891
AfiI CCNNNNNNNGG 4 cut(s) 453, 700, 844, 1043
AflIII ACRYGT 1 cut(s) 263
AgeI ACCGGT 1 cut(s) 138
AgsI TTSAA 4 cut(s) 503, 538, 556, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 8 cut(s) 46, 80, 112, 531, 780, 884, 915, 1021
AluI AGCT 8 cut(s) 46, 80, 112, 531, 780, 884, 915, 1021
Alw21I GWGCWC 1 cut(s) 693
Alw26I GTCTC 5 cut(s) 100, 137, 391, 604, 664
AlwI GGATC 1 cut(s) 641
AlwNI CAGNNNCTG 1 cut(s) 790
AoxI GGCC 2 cut(s) 3, 640
ApeKI GCWGC 1 cut(s) 790
ApoI RAATTY 4 cut(s) 21, 209, 538, 848
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
AsiGI ACCGGT 1 cut(s) 138
AspLEI GCGC 1 cut(s) 936
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 165
AsuHPI GGTGA 4 cut(s) 47, 269, 718, 899
AsuII TTCGAA 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 986
BanI GGYRCC 1 cut(s) 981
BanII GRGCYC 1 cut(s) 823
BbsI GAAGAC 3 cut(s) 146, 332, 507
Bbv12I GWGCWC 1 cut(s) 693
BbvI GCAGC 1 cut(s) 777
BccI CCATC 1 cut(s) 994
BcnI CCSGG 1 cut(s) 165
BcoDI GTCTC 5 cut(s) 100, 137, 391, 604, 664
BfaI CTAG 7 cut(s) 32, 47, 203, 584, 912, 1022, 1056
BisI GCNGC 2 cut(s) 791, 1037
BlsI GCNGC 2 cut(s) 792, 1038
Bme1390I CCNGG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 983
BmrFI CCNGG 1 cut(s) 165
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BpiI GAAGAC 3 cut(s) 146, 332, 507
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 977
Bpu14I TTCGAA 1 cut(s) 213
BpuEI CTTGAG 2 cut(s) 296, 596
BpuMI CCSGG 1 cut(s) 165
BsaAI YACGTR 1 cut(s) 893
BsaI GGTCTC 2 cut(s) 100, 664
BsaWI WCCGGW 1 cut(s) 138
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 4 cut(s) 453, 700, 844, 1043
Bse118I RCCGGY 1 cut(s) 138
Bse1I ACTGG 3 cut(s) 307, 519, 883
Bse3DI GCAATG 2 cut(s) 679, 756
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 4 cut(s) 453, 700, 844, 1043
BseMI GCAATG 2 cut(s) 679, 756
BseMII CTCAG 2 cut(s) 246, 358
BseNI ACTGG 3 cut(s) 307, 519, 883
BseSI GKGCMC 1 cut(s) 986
BseXI GCAGC 1 cut(s) 777
BseYI CCCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 2 cut(s) 5, 642
BshNI GGYRCC 1 cut(s) 981
BshTI ACCGGT 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 693
BsiSI CCGG 2 cut(s) 139, 165
BslFI GGGAC 2 cut(s) 156, 1060
BslI CCNNNNNNNGG 4 cut(s) 453, 700, 844, 1043
BsmAI GTCTC 5 cut(s) 100, 137, 391, 604, 664
BsmFI GGGAC 2 cut(s) 156, 1060
BsnI GGCC 2 cut(s) 5, 642
Bso31I GGTCTC 2 cut(s) 100, 664
Bsp119I TTCGAA 1 cut(s) 213
Bsp1286I GDGCHC 3 cut(s) 693, 823, 986
Bsp143I GATC 5 cut(s) 82, 241, 565, 633, 723
BspACI CCGC 3 cut(s) 223, 570, 1037
BspANI GGCC 2 cut(s) 5, 642
BspCNI CTCAG 2 cut(s) 245, 357
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 1 cut(s) 983
BspPI GGATC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 213
BspT107I GGYRCC 1 cut(s) 981
BspTNI GGTCTC 2 cut(s) 100, 664
BsrBI CCGCTC 1 cut(s) 225
BsrDI GCAATG 2 cut(s) 679, 756
BsrFI RCCGGY 1 cut(s) 138
BsrI ACTGG 3 cut(s) 307, 519, 883
BssAI RCCGGY 1 cut(s) 138
BssMI GATC 5 cut(s) 82, 241, 565, 633, 723
Bst4CI ACNGT 2 cut(s) 239, 710
BstAPI GCANNNNNTGC 3 cut(s) 676, 790, 1033
BstBAI YACGTR 1 cut(s) 893
BstBI TTCGAA 1 cut(s) 213
BstC8I GCNNGC 2 cut(s) 110, 782
BstDEI CTNAG 4 cut(s) 232, 344, 695, 977
BstEII GGTNACC 1 cut(s) 905
BstF5I GGATG 1 cut(s) 46
BstFNI CGCG 1 cut(s) 570
BstHHI GCGC 1 cut(s) 936
BstKTI GATC 5 cut(s) 85, 244, 568, 636, 726
BstMAI GTCTC 5 cut(s) 100, 137, 391, 604, 664
BstMBI GATC 5 cut(s) 82, 241, 565, 633, 723
BstMWI GCNNNNNNNGC 3 cut(s) 676, 790, 1033
BstNSI RCATGY 1 cut(s) 267
BstPI GGTNACC 1 cut(s) 905
BstSCI CCNGG 1 cut(s) 163
BstSLI GKGCMC 1 cut(s) 986
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 1 cut(s) 777
BstV2I GAAGAC 3 cut(s) 146, 332, 507
BstXI CCANNNNNNTGG 1 cut(s) 14
BsuRI GGCC 2 cut(s) 5, 642
BtsCI GGATG 1 cut(s) 46
Cac8I GCNNGC 2 cut(s) 110, 782
CaiI CAGNNNCTG 1 cut(s) 790
CfoI GCGC 1 cut(s) 936
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 1057
Csp6I GTAC 2 cut(s) 419, 890
CspAI ACCGGT 1 cut(s) 138
CviAII CATG 1 cut(s) 264
CviQI GTAC 2 cut(s) 419, 890
DdeI CTNAG 4 cut(s) 232, 344, 695, 977
DpnI GATC 5 cut(s) 84, 243, 567, 635, 725
DpnII GATC 5 cut(s) 82, 241, 565, 633, 723
DrdI GACNNNNNNGTC 1 cut(s) 56
DseDI GACNNNNNNGTC 1 cut(s) 56
Eco147I AGGCCT 1 cut(s) 642
Eco24I GRGCYC 1 cut(s) 823
Eco31I GGTCTC 2 cut(s) 100, 664
Eco91I GGTNACC 1 cut(s) 905
EcoO65I GGTNACC 1 cut(s) 905
EcoRI GAATTC 1 cut(s) 848
EcoT22I ATGCAT 1 cut(s) 681
EcoT38I GRGCYC 1 cut(s) 823
FaeI CATG 1 cut(s) 267
FaiI YATR 6 cut(s) 115, 120, 265, 383, 405, 434
FaqI GGGAC 2 cut(s) 156, 1060
FatI CATG 1 cut(s) 263
FauI CCCGC 2 cut(s) 230, 563
FblI GTMKAC 1 cut(s) 948
Fnu4HI GCNGC 2 cut(s) 791, 1037
FokI GGATG 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 823
Fsp4HI GCNGC 2 cut(s) 791, 1037
FspBI CTAG 7 cut(s) 32, 47, 203, 584, 912, 1022, 1056
GlaI GCGC 1 cut(s) 935
GluI GCNGC 2 cut(s) 791, 1037
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 2 cut(s) 5, 642
HapII CCGG 2 cut(s) 139, 165
HgaI GACGC 1 cut(s) 1057
HhaI GCGC 1 cut(s) 936
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HincII GTYRAC 3 cut(s) 61, 286, 949
HindII GTYRAC 3 cut(s) 61, 286, 949
HinfI GANTC 2 cut(s) 130, 199
HpaII CCGG 2 cut(s) 139, 165
HphI GGTGA 4 cut(s) 47, 269, 718, 899
Hpy166II GTNNAC 4 cut(s) 61, 286, 890, 949
Hpy188I TCNGA 7 cut(s) 87, 100, 235, 721, 805, 831, 958
Hpy188III TCNNGA 3 cut(s) 556, 613, 751
Hpy8I GTNNAC 4 cut(s) 61, 286, 890, 949
Hpy99I CGWCG 1 cut(s) 953
HpyCH4III ACNGT 2 cut(s) 239, 710
HpyCH4IV ACGT 2 cut(s) 892, 951
HpyCH4V TGCA 7 cut(s) 108, 403, 670, 679, 784, 793, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 676, 790, 1033
HpyF3I CTNAG 4 cut(s) 232, 344, 695, 977
HpySE526I ACGT 2 cut(s) 892, 951
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 1 cut(s) 934
Kzo9I GATC 5 cut(s) 82, 241, 565, 633, 723
LmnI GCTCC 3 cut(s) 166, 688, 881
Lsp1109I GCAGC 1 cut(s) 777
MaeI CTAG 7 cut(s) 32, 47, 203, 584, 912, 1022, 1056
MaeII ACGT 2 cut(s) 892, 951
MaeIII GTNAC 2 cut(s) 484, 905
MalI GATC 5 cut(s) 84, 243, 567, 635, 725
MbiI CCGCTC 1 cut(s) 225
MboI GATC 5 cut(s) 82, 241, 565, 633, 723
MboII GAAGA 3 cut(s) 146, 332, 507
MfeI CAATTG 1 cut(s) 507
MhlI GDGCHC 3 cut(s) 693, 823, 986
MluCI AATT 7 cut(s) 21, 209, 448, 507, 538, 832, 848
MlyI GAGTC 1 cut(s) 139
Mph1103I ATGCAT 1 cut(s) 681
MseI TTAA 2 cut(s) 387, 860
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 2 cut(s) 139, 165
MspR9I CCNGG 1 cut(s) 165
MunI CAATTG 1 cut(s) 507
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 3 cut(s) 676, 790, 1033
NciI CCSGG 1 cut(s) 165
NdeII GATC 5 cut(s) 82, 241, 565, 633, 723
NlaIII CATG 1 cut(s) 267
NlaIV GGNNCC 1 cut(s) 983
NmuCI GTSAC 2 cut(s) 484, 905
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 1 cut(s) 267
NspV TTCGAA 1 cut(s) 213
PceI AGGCCT 1 cut(s) 642
PciI ACATGT 1 cut(s) 263
PfeI GAWTC 1 cut(s) 199
PinAI ACCGGT 1 cut(s) 138
PkrI GCNGC 2 cut(s) 792, 1038
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
Ppu21I YACGTR 1 cut(s) 893
PscI ACATGT 1 cut(s) 263
PsiI TTATAA 1 cut(s) 434
PspEI GGTNACC 1 cut(s) 905
PspFI CCCAGC 1 cut(s) 76
PspN4I GGNNCC 1 cut(s) 983
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 790
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 2 cut(s) 420, 891
RsaNI GTAC 2 cut(s) 419, 890
SalI GTCGAC 1 cut(s) 947
SaqAI TTAA 2 cut(s) 387, 860
SatI GCNGC 2 cut(s) 791, 1037
Sau3AI GATC 5 cut(s) 82, 241, 565, 633, 723
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 3 cut(s) 693, 823, 986
SfuI TTCGAA 1 cut(s) 213
SmlI CTYRAG 2 cut(s) 275, 611
SmoI CTYRAG 2 cut(s) 275, 611
Sse9I AATT 7 cut(s) 21, 209, 448, 507, 538, 832, 848
SseBI AGGCCT 1 cut(s) 642
SsiI CCGC 3 cut(s) 223, 570, 1037
SspMI CTAG 7 cut(s) 32, 47, 203, 584, 912, 1022, 1056
StuI AGGCCT 1 cut(s) 642
StyD4I CCNGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 239, 710
TaiI ACGT 2 cut(s) 895, 954
TaqI TCGA 5 cut(s) 213, 524, 752, 855, 948
TaqII GACCGA 1 cut(s) 82
TasI AATT 7 cut(s) 21, 209, 448, 507, 538, 832, 848
TauI GCSGC 1 cut(s) 1039
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 2 cut(s) 387, 860
Tru9I TTAA 2 cut(s) 387, 860
TseFI GTSAC 2 cut(s) 484, 905
TseI GCWGC 1 cut(s) 790
Tsp45I GTSAC 2 cut(s) 484, 905
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 2 cut(s) 503, 934
XapI RAATTY 4 cut(s) 21, 209, 538, 848
XceI RCATGY 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 948
XspI CTAG 7 cut(s) 32, 47, 203, 584, 912, 1022, 1056
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.