FvH4_3g20730

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000341
Physical Location & Seq
Reverse (-)
13775747 .. 13776978
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20730.t1

Sequence Viewer

Length: 1014 bp
ATGGCTTCTCATGAACCCATTAACCTTGTGTTCATTGTCATATTCATCACCTTGGCAACTTTGGCTTCTCATGCCACATCCCCTGCATTGTACGAAGCTTCCCTTGCTGAAAAACACGAGCAATGGATGGCAAAGCACGGACGCGTTTACCCCGATGCTGCAGAGAAGGAACGGCGTCTCGGCATCTTCAAGAACAATGTAGAATACGTCGAGAAGTTCAACAAAGGGAACAAGACATACACGTTGAGCATAAACAACTTGGCTGCTTCTGATATTCCAACTAGAATGGACTGGAGGGAACAACAAGCTGTCACTCCCATAAAGGATCAAGGTCGATGCGGTGCTTGCTGGGCATTTTCAGTAGCAGCAGCTGTGGAAGGGCTAACCAAAATCAAAACCGGCGAATTGATCTCACTCTCCGAGCAGCAACTTGTGGACTGCAGCTCCCAGAACAATGGGTGCGGCGGTGGTAGCTTGAAACTCGCATATGACTACGTAGAGCAAAACGGACTGGCTCGTGAAGAAAGTTACCCGTACGAGGGAACAGACACTGGAACATGCAACACTGACATGGAAAGTGAGCGTGCGGCAAAGATCACTAGCTATGCCCAAGTCACTTCCAGAGATGAAAACGATCTACTCAAGGCTGTGGCCATGCAACCAGTCTCGATCCGCATTGCGGCTTATGGATTGGATTTTCAGCAATACAGCATCGGGGTGTTCTCAGGCGATTATGGGACGGACCTCAACCATGACATCACGGCCATCGGCTACGGGACAACTGAAGATGGAATCGACTATTGGTTAATGAAAAACTCATGGGGGACGCAATGGGGTGAAAGTGGGTTTATGAAAATTCTTAGAAACAGTGACGCCCCAGAGGGTATGTGCGGCCTTGCTATTAATAACTTCGATTGCATAAGTCTCTACGTATACTACAGCATGCCTCTTTTCCATATTTATCTTTCTATTGAGTATGCACTTCCAAATTCTACTATACATGTTGGGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

338

Amino Acids

37.29

Weight (kDa)

4.88

Isoelectric Point (pI)

28.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 39 - 89 8.9e-16 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 92 - 301 1.7e-73 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 933
AccII CGCG 1 cut(s) 144
AciI CCGC 7 cut(s) 339, 462, 465, 587, 673, 680, 891
AclWI GGATC 2 cut(s) 333, 664
AcoI YGGCCR 2 cut(s) 651, 762
AcsI RAATTY 2 cut(s) 855, 988
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 2 cut(s) 175, 873
AfaI GTAC 2 cut(s) 92, 536
AfiI CCNNNNNNNGG 3 cut(s) 538, 539, 679
AflIII ACRYGT 3 cut(s) 142, 240, 1000
AgsI TTSAA 3 cut(s) 190, 220, 478
AjuI GAANNNNNNNTTGG 2 cut(s) 784, 816
AluBI AGCT 6 cut(s) 98, 308, 371, 444, 474, 603
AluI AGCT 6 cut(s) 98, 308, 371, 444, 474, 603
Alw26I GTCTC 3 cut(s) 182, 670, 929
AlwI GGATC 2 cut(s) 333, 664
AlwNI CAGNNNCTG 2 cut(s) 371, 551
AoxI GGCC 3 cut(s) 651, 762, 892
ApeKI GCWGC 6 cut(s) 158, 263, 365, 368, 424, 441
ApoI RAATTY 2 cut(s) 855, 988
AseI ATTAAT 1 cut(s) 903
AspS9I GGNCC 1 cut(s) 742
AsuHPI GGTGA 2 cut(s) 40, 848
AvaII GGWCC 1 cut(s) 742
BalI TGGCCA 1 cut(s) 653
BauI CACGAG 2 cut(s) 116, 516
BbvI GCAGC 6 cut(s) 145, 250, 377, 380, 436, 453
BccI CCATC 3 cut(s) 121, 773, 782
BceAI ACGGC 2 cut(s) 188, 777
BcoDI GTCTC 3 cut(s) 182, 670, 929
BfaI CTAG 2 cut(s) 282, 600
BfmI CTRYAG 3 cut(s) 159, 439, 937
Bme18I GGWCC 1 cut(s) 742
BmgT120I GGNCC 1 cut(s) 742
BmsI GCATC 4 cut(s) 145, 192, 326, 720
BpmI CTGGAG 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 626
BsaAI YACGTR 2 cut(s) 496, 931
BsaHI GRCGYC 2 cut(s) 175, 873
BsaJI CCNNGG 1 cut(s) 51
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 3 cut(s) 538, 539, 679
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 4 cut(s) 296, 516, 556, 662
Bse3DI GCAATG 3 cut(s) 128, 675, 836
BseDI CCNNGG 1 cut(s) 51
BseGI GGATG 2 cut(s) 77, 132
BseLI CCNNNNNNNGG 3 cut(s) 538, 539, 679
BseMI GCAATG 3 cut(s) 128, 675, 836
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 4 cut(s) 296, 516, 556, 662
BseXI GCAGC 6 cut(s) 145, 250, 377, 380, 436, 453
BseYI CCCAGC 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 3 cut(s) 653, 764, 894
BsiSI CCGG 1 cut(s) 399
BsiWI CGTACG 1 cut(s) 534
BslFI GGGAC 3 cut(s) 751, 790, 838
BslI CCNNNNNNNGG 3 cut(s) 538, 539, 679
BsmAI GTCTC 3 cut(s) 182, 670, 929
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 3 cut(s) 751, 790, 838
BsnI GGCC 3 cut(s) 653, 764, 894
Bsp143I GATC 5 cut(s) 325, 408, 594, 634, 669
BspACI CCGC 7 cut(s) 339, 462, 465, 587, 673, 680, 891
BspANI GGCC 3 cut(s) 653, 764, 894
BspCNI CTCAG 1 cut(s) 737
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 10
BspMAI CTGCAG 2 cut(s) 163, 443
BspPI GGATC 2 cut(s) 333, 664
BsrDI GCAATG 3 cut(s) 128, 675, 836
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 4 cut(s) 296, 516, 556, 662
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 5 cut(s) 325, 408, 594, 634, 669
BssNAI GTATAC 1 cut(s) 934
BssNI GRCGYC 2 cut(s) 175, 873
BssSI CACGAG 2 cut(s) 116, 516
BssT1I CCWWGG 1 cut(s) 51
Bst1107I GTATAC 1 cut(s) 934
Bst2BI CACGAG 2 cut(s) 116, 516
Bst4CI ACNGT 1 cut(s) 869
BstACI GRCGYC 2 cut(s) 175, 873
BstBAI YACGTR 2 cut(s) 496, 931
BstC8I GCNNGC 3 cut(s) 346, 585, 944
BstDEI CTNAG 2 cut(s) 724, 860
BstF5I GGATG 2 cut(s) 77, 132
BstFNI CGCG 1 cut(s) 144
BstKTI GATC 5 cut(s) 328, 411, 597, 637, 672
BstMAI GTCTC 3 cut(s) 182, 670, 929
BstMBI GATC 5 cut(s) 325, 408, 594, 634, 669
BstMWI GCNNNNNNNGC 6 cut(s) 62, 71, 104, 345, 350, 471
BstNSI RCATGY 3 cut(s) 561, 946, 1004
BstSFI CTRYAG 3 cut(s) 159, 439, 937
BstSNI TACGTA 2 cut(s) 496, 931
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 6 cut(s) 145, 250, 377, 380, 436, 453
BstXI CCANNNNNNTGG 1 cut(s) 455
BstZ17I GTATAC 1 cut(s) 934
BsuRI GGCC 3 cut(s) 653, 764, 894
BtsCI GGATG 2 cut(s) 77, 132
BtsIMutI CAGTG 3 cut(s) 549, 564, 874
Cac8I GCNNGC 3 cut(s) 346, 585, 944
CaiI CAGNNNCTG 2 cut(s) 371, 551
CciI TCATGA 1 cut(s) 10
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 1 cut(s) 742
CseI GACGC 4 cut(s) 150, 164, 835, 881
Csp6I GTAC 2 cut(s) 91, 535
CviAII CATG 9 cut(s) 11, 71, 558, 571, 655, 752, 819, 943, 1001
CviQI GTAC 2 cut(s) 91, 535
DdeI CTNAG 2 cut(s) 724, 860
DpnI GATC 5 cut(s) 327, 410, 596, 636, 671
DpnII GATC 5 cut(s) 325, 408, 594, 634, 669
EaeI YGGCCR 2 cut(s) 651, 762
Eco105I TACGTA 2 cut(s) 496, 931
Eco130I CCWWGG 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 742
Eco57I CTGAAG 1 cut(s) 804
EcoT14I CCWWGG 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 9 cut(s) 14, 74, 561, 574, 658, 755, 822, 946, 1004
FalI AAGNNNNNCTT 2 cut(s) 87, 119
FaqI GGGAC 3 cut(s) 751, 790, 838
FatI CATG 9 cut(s) 10, 70, 557, 570, 654, 751, 818, 942, 1000
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 933
FokI GGATG 2 cut(s) 64, 139
FspBI CTAG 2 cut(s) 282, 600
GsaI CCCAGC 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 313
HaeIII GGCC 3 cut(s) 653, 764, 894
HapII CCGG 1 cut(s) 399
HgaI GACGC 4 cut(s) 150, 164, 835, 881
Hin1I GRCGYC 2 cut(s) 175, 873
Hin1II CATG 9 cut(s) 14, 74, 561, 574, 658, 755, 822, 946, 1004
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 1 cut(s) 792
HpaII CCGG 1 cut(s) 399
HphI GGTGA 2 cut(s) 40, 848
Hpy166II GTNNAC 3 cut(s) 148, 436, 934
Hpy188I TCNGA 2 cut(s) 271, 421
Hpy188III TCNNGA 7 cut(s) 11, 190, 211, 518, 621, 667, 1011
Hpy8I GTNNAC 3 cut(s) 148, 436, 934
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 2 cut(s) 160, 371
HpyCH4III ACNGT 1 cut(s) 869
HpyCH4IV ACGT 4 cut(s) 207, 242, 495, 930
HpyCH4V TGCA 7 cut(s) 86, 161, 441, 561, 658, 918, 980
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 71, 104, 345, 350, 471
HpyF3I CTNAG 2 cut(s) 724, 860
HpySE526I ACGT 4 cut(s) 207, 242, 495, 930
Hsp92I GRCGYC 2 cut(s) 175, 873
Hsp92II CATG 9 cut(s) 14, 74, 561, 574, 658, 755, 822, 946, 1004
Kzo9I GATC 5 cut(s) 325, 408, 594, 634, 669
LmnI GCTCC 1 cut(s) 449
Lsp1109I GCAGC 6 cut(s) 145, 250, 377, 380, 436, 453
LweI GCATC 4 cut(s) 145, 192, 326, 720
MaeI CTAG 2 cut(s) 282, 600
MaeII ACGT 4 cut(s) 207, 242, 495, 930
MaeIII GTNAC 4 cut(s) 310, 527, 613, 869
MalI GATC 5 cut(s) 327, 410, 596, 636, 671
MboI GATC 5 cut(s) 325, 408, 594, 634, 669
MboII GAAGA 3 cut(s) 178, 533, 797
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 3 cut(s) 404, 855, 988
MluI ACGCGT 1 cut(s) 142
MluNI TGGCCA 1 cut(s) 653
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 5 cut(s) 288, 532, 755, 874, 957
Mox20I TGGCCA 1 cut(s) 653
MscI TGGCCA 1 cut(s) 653
MseI TTAA 3 cut(s) 21, 806, 903
MslI CAYNNNNRTG 2 cut(s) 569, 716
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 399
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 6 cut(s) 62, 71, 104, 345, 350, 471
NdeI CATATG 1 cut(s) 487
NdeII GATC 5 cut(s) 325, 408, 594, 634, 669
NlaIII CATG 9 cut(s) 14, 74, 561, 574, 658, 755, 822, 946, 1004
NmeAIII GCCGAG 1 cut(s) 159
NmuCI GTSAC 3 cut(s) 310, 613, 869
NspI RCATGY 3 cut(s) 561, 946, 1004
PaeI GCATGC 1 cut(s) 946
PagI TCATGA 1 cut(s) 10
PciI ACATGT 1 cut(s) 1000
PfeI GAWTC 1 cut(s) 792
Pfl23II CGTACG 1 cut(s) 534
Ppu21I YACGTR 2 cut(s) 496, 931
PscI ACATGT 1 cut(s) 1000
PshBI ATTAAT 1 cut(s) 903
PspFI CCCAGC 1 cut(s) 348
PspLI CGTACG 1 cut(s) 534
PspPI GGNCC 1 cut(s) 742
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PstI CTGCAG 2 cut(s) 163, 443
PstNI CAGNNNCTG 2 cut(s) 371, 551
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 2 cut(s) 92, 536
RsaNI GTAC 2 cut(s) 91, 535
RseI CAYNNNNRTG 2 cut(s) 569, 716
SaqAI TTAA 3 cut(s) 21, 806, 903
Sau3AI GATC 5 cut(s) 325, 408, 594, 634, 669
Sau96I GGNCC 1 cut(s) 742
SfaNI GCATC 4 cut(s) 145, 192, 326, 720
SfcI CTRYAG 3 cut(s) 159, 439, 937
SinI GGWCC 1 cut(s) 742
SmiMI CAYNNNNRTG 2 cut(s) 569, 716
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
SnaBI TACGTA 2 cut(s) 496, 931
SphI GCATGC 1 cut(s) 946
Sse9I AATT 3 cut(s) 404, 855, 988
SsiI CCGC 7 cut(s) 339, 462, 465, 587, 673, 680, 891
SspMI CTAG 2 cut(s) 282, 600
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 869
TaiI ACGT 4 cut(s) 210, 245, 498, 933
TaqI TCGA 5 cut(s) 210, 334, 668, 795, 912
TasI AATT 3 cut(s) 404, 855, 988
TauI GCSGC 4 cut(s) 465, 590, 683, 894
TfiI GAWTC 1 cut(s) 792
Tru1I TTAA 3 cut(s) 21, 806, 903
Tru9I TTAA 3 cut(s) 21, 806, 903
TscAI CASTG 3 cut(s) 556, 571, 874
TseFI GTSAC 3 cut(s) 310, 613, 869
TseI GCWGC 6 cut(s) 158, 263, 365, 368, 424, 441
Tsp45I GTSAC 3 cut(s) 310, 613, 869
TspDTI ATGAA 6 cut(s) 22, 27, 34, 642, 824, 866
TspGWI ACGGA 3 cut(s) 153, 522, 755
TspRI CASTG 3 cut(s) 556, 571, 874
VpaK11BI GGWCC 1 cut(s) 742
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 2 cut(s) 855, 988
XceI RCATGY 3 cut(s) 561, 946, 1004
XmiI GTMKAC 1 cut(s) 933
XspI CTAG 2 cut(s) 282, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.