pycom10g23510

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000341
Physical Location & Seq
Forward (+)
25635642 .. 25636316
675 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g23510.1

Sequence Viewer

Length: 552 bp
ATGGCTTTTGAAAGAAACCTTTTGATTGTTACCATACTCATCACCTTGGGGACCTTGGCATCTGAAGCCACGTCCCGCACATTGTACCAAGCTTCCATTGCTGCAAAACATGTGCAGTGGATGAAAAAGCATGGACGCGTTTACCAGGACAGCACAGAGAAGGAAAGGCGTTTCCAGATTTTCAAAAACAATGTGGAGTTTATCGAGAAATTCAACAGTGAAGGGAACATGACTTACAGCCTATGCATCAACCGATTTTCGGATATGACCTATGATGAATTCCTCAGGCATTATACTGGATACAAGCAGCCCACTGAATCAACCTCATCCGCAGCTGCATCTTTTACATACGAAAACCTAAGCCCAGCTGATGTTCCCCAAAGCATTGACTGGAGGGAGAAGAGAGCAGTTACTCCAGTAAAATTTCAAGGGAGCTGTGGTAAGAGCAGGTTGTTGCTGGGCATTTGCCGCAGTGGCAGCAGTCGAAGGGATTACCCAAATCAAAACTGGCAAATTGATCTCACTGTCCGAGCAACAACTTGTGGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

184

Amino Acids

20.98

Weight (kDa)

9.21

Isoelectric Point (pI)

49.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 38 - 94 8e-20 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 438
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 76, 330, 469
AcsI RAATTY 3 cut(s) 209, 278, 422
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 259
AflIII ACRYGT 2 cut(s) 109, 136
AgsI TTSAA 4 cut(s) 11, 184, 214, 428
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 4 cut(s) 92, 335, 368, 435
AluI AGCT 4 cut(s) 92, 335, 368, 435
ApeKI GCWGC 5 cut(s) 101, 307, 332, 335, 477
ApoI RAATTY 3 cut(s) 209, 278, 422
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 51
AxyI CCTNAGG 1 cut(s) 284
BbvI GCAGC 5 cut(s) 88, 319, 322, 344, 489
BciT130I CCWGG 1 cut(s) 146
BciVI GTATCC 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 438
BfuI GTATCC 1 cut(s) 293
BglI GCCNNNNNGGC 1 cut(s) 474
BisI GCNGC 6 cut(s) 102, 308, 333, 336, 469, 478
BlsI GCNGC 6 cut(s) 103, 309, 334, 337, 470, 479
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 3 cut(s) 68, 255, 347
BpmI CTGGAG 2 cut(s) 399, 412
Bpu10I CCTNAGC 1 cut(s) 359
BsaJI CCNNGG 2 cut(s) 45, 54
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse1I ACTGG 4 cut(s) 301, 395, 416, 512
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 45, 54
BseGI GGATG 2 cut(s) 126, 326
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 298
BseNI ACTGG 4 cut(s) 301, 395, 416, 512
BseXI GCAGC 5 cut(s) 88, 319, 322, 344, 489
BseYI CCCAGC 2 cut(s) 364, 457
BsgI GTGCAG 1 cut(s) 134
Bsh1236I CGCG 1 cut(s) 138
BslFI GGGAC 2 cut(s) 58, 64
BslI CCNNNNNNNGG 1 cut(s) 259
BsmFI GGGAC 2 cut(s) 58, 64
Bsp143I GATC 1 cut(s) 517
BspACI CCGC 3 cut(s) 76, 330, 469
BspCNI CTCAG 1 cut(s) 297
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 4 cut(s) 301, 395, 416, 512
BssECI CCNNGG 2 cut(s) 45, 54
BssMI GATC 1 cut(s) 517
BssT1I CCWWGG 2 cut(s) 45, 54
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 218, 526, 549
Bst6I CTCTTC 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 284, 359
BstF5I GGATG 2 cut(s) 126, 326
BstFNI CGCG 1 cut(s) 138
BstKTI GATC 1 cut(s) 520
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 5 cut(s) 65, 98, 468, 474, 477
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 5 cut(s) 88, 319, 322, 344, 489
Bsu36I CCTNAGG 1 cut(s) 284
BsuI GTATCC 1 cut(s) 293
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 2 cut(s) 126, 326
BtsI GCAGTG 2 cut(s) 122, 478
BtsIMutI CAGTG 5 cut(s) 122, 223, 312, 478, 522
BveI ACCTGC 1 cut(s) 438
Cfr13I GGNCC 1 cut(s) 51
CseI GACGC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 85
CviAII CATG 3 cut(s) 110, 131, 229
CviJI RGCY 9 cut(s) 5, 68, 92, 240, 310, 335, 363, 368, 435
CviKI_1 RGCY 9 cut(s) 5, 68, 92, 240, 310, 335, 363, 368, 435
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 2 cut(s) 284, 359
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Eco130I CCWWGG 2 cut(s) 45, 54
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 84
Eco81I CCTNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 45, 54
EcoT22I ATGCAT 1 cut(s) 248
ErhI CCWWGG 2 cut(s) 45, 54
FaeI CATG 3 cut(s) 113, 134, 232
FaiI YATR 9 cut(s) 35, 111, 132, 230, 244, 266, 273, 294, 349
FaqI GGGAC 2 cut(s) 58, 64
FatI CATG 3 cut(s) 109, 130, 228
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 6 cut(s) 102, 308, 333, 336, 469, 478
FokI GGATG 2 cut(s) 133, 313
Fsp4HI GCNGC 6 cut(s) 102, 308, 333, 336, 469, 478
GluI GCNGC 6 cut(s) 102, 308, 333, 336, 469, 478
GsaI CCCAGC 2 cut(s) 368, 461
GsuI CTGGAG 2 cut(s) 399, 412
HgaI GACGC 1 cut(s) 144
Hin1II CATG 3 cut(s) 113, 134, 232
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 1 cut(s) 317
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 2 cut(s) 142, 545
Hpy188I TCNGA 3 cut(s) 64, 262, 530
Hpy188III TCNNGA 2 cut(s) 175, 205
Hpy8I GTNNAC 2 cut(s) 142, 545
HpyAV CCTTC 3 cut(s) 154, 215, 480
HpyCH4III ACNGT 3 cut(s) 218, 526, 549
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 4 cut(s) 104, 115, 246, 338
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 98, 468, 474, 477
HpyF3I CTNAG 2 cut(s) 284, 359
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 3 cut(s) 113, 134, 232
Kzo9I GATC 1 cut(s) 517
LmnI GCTCC 1 cut(s) 432
Lsp1109I GCAGC 5 cut(s) 88, 319, 322, 344, 489
LweI GCATC 3 cut(s) 68, 255, 347
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 2 cut(s) 28, 409
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 1 cut(s) 412
MluCI AATT 4 cut(s) 209, 278, 422, 513
MluI ACGCGT 1 cut(s) 136
MnlI CCTC 3 cut(s) 293, 334, 387
Mph1103I ATGCAT 1 cut(s) 248
MspA1I CMGCKG 2 cut(s) 335, 368
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 5 cut(s) 65, 98, 468, 474, 477
NdeII GATC 1 cut(s) 517
NlaIII CATG 3 cut(s) 113, 134, 232
NlaIV GGNNCC 1 cut(s) 52
NsiI ATGCAT 1 cut(s) 248
NspI RCATGY 1 cut(s) 113
PciI ACATGT 1 cut(s) 109
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 6 cut(s) 103, 309, 334, 337, 470, 479
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 144
PspFI CCCAGC 2 cut(s) 364, 457
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 51
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 218, 250
PvuII CAGCTG 2 cut(s) 335, 368
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SatI GCNGC 6 cut(s) 102, 308, 333, 336, 469, 478
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 146
SfaNI GCATC 3 cut(s) 68, 255, 347
SinI GGWCC 1 cut(s) 51
Sse9I AATT 4 cut(s) 209, 278, 422, 513
SsiI CCGC 3 cut(s) 76, 330, 469
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 2 cut(s) 45, 54
TaaI ACNGT 3 cut(s) 218, 526, 549
TaiI ACGT 1 cut(s) 74
TaqI TCGA 2 cut(s) 204, 484
TasI AATT 4 cut(s) 209, 278, 422, 513
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 1 cut(s) 317
TscAI CASTG 5 cut(s) 122, 223, 319, 478, 529
TseI GCWGC 5 cut(s) 101, 307, 332, 335, 477
TspDTI ATGAA 2 cut(s) 137, 291
TspRI CASTG 5 cut(s) 122, 223, 319, 478, 529
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 3 cut(s) 209, 278, 422
XceI RCATGY 1 cut(s) 113
XcmI CCANNNNNNNNNTGG 1 cut(s) 504
Zsp2I ATGCAT 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.