Rh5AG232900

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000341
Physical Location & Seq
Forward (+)
28127491 .. 28128778
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG232900.1

Sequence Viewer

Length: 1176 bp
ATGGCTTTTCAACGCATTAACCTTGTGTTTATTTCCATATTCATTACCTTGGGGACTTTGGCTTCTCATGCCACATCCCCTTCGTTGTATGAAGCTTCCCTTGCTGAAAAACATGAGCAATGGATGGCAAAGCACGGACGCGTTTACCCCAATGCGGCAGAGAAGGAAATGCGTTTCGGCATCTTCAGGAAGAATGTCGAATACGTGGAGAAGTTCAGCAATGAAGCGAATAAGACCTACAAGTTGAACATCAACAGGTTTTCTGATATGACCAACGAGGAATTCCGGAGACATCTCACCGGATTCAAAATGCCCCCCGGCTTAAGTTCAACGTCCTCCAATTTTAGCTACCAAAGCTTGGCTGCTACAGATATTCCAACTAGTGTGGACTGGAGGGAACAACAAGCTGTCACCCCCATAAAAGATCAAAATTCATGCGATGCTAGCTGGGCATTTACAGTAGCAGCAGCTGTGGAAGGGCTAACCAAAATCACAACCGGCCAATTGATGTCACTCTCCGAGCAGCAACTTGTGGACTGCAATCATCAGAGCTCTGGGTGCAACGGTAGTAGCTTAGAATCCGCCTATTCCTACGTAGTACAAAACGGAGGACTCGCTCGTGAAGCAACTTACCCCTACCAGGCAACAGACATGGGAACATGCGATACTAACAAGGAAAGTGAGCATGCGGGACAGATCACTGGCTATGAACGAGTCCCTTCCAGAAGTGAAAACGATCTACAGAAGGCTGTGGCCATGCAACCAGTATCGGTTGGCATTACTGCTAATGGACAAGATTTTCAAATGTACCGCAGTGGGGTGTTCTCCGGCAATTGTGGGACGGGGATCGACCACGCCGTCACGGCCATTGGCTACGGGACGACTGAAGATGGAACCCCCTATTGGTTAATGAAGAACTCATGGGGGGAGGGTTGGGGTGAAAGTGGGTATATGAGGATTCTTAGAAACAGTGATGCCCCAGAGGGTATGTGCGGCCTTGCTCTGAATTCTGTCTATCCGACTGCTCCAGGACTTCCACCTAAGCCGACTGCTCCAGCACTTCCACCTAAGCCTACTGCTCCAGCACTTCCACCTAAGCCAACTGCTCCAGGACTTCCACCTAAGCAACACTATGCCAAAAACTGCATCAGACGACAGACAGAAACTGTTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

391

Amino Acids

42.72

Weight (kDa)

6.27

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 38 - 95 1.3e-17 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 340 2.7e-74 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
AccB7I CCANNNNNTGG 1 cut(s) 358
AccII CGCG 1 cut(s) 141
AccIII TCCGGA 1 cut(s) 285
AciI CCGC 5 cut(s) 155, 582, 689, 811, 993
AclWI GGATC 1 cut(s) 854
AcoI YGGCCR 3 cut(s) 499, 753, 864
AcsI RAATTY 3 cut(s) 281, 430, 1006
AcuI CTGAAG 2 cut(s) 169, 906
AfaI GTAC 2 cut(s) 600, 809
AfiI CCNNNNNNNGG 5 cut(s) 154, 358, 640, 817, 903
AflII CTTAAG 1 cut(s) 322
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 5 cut(s) 11, 247, 307, 330, 803
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 3 cut(s) 639, 1027, 1108
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 886, 918
AluBI AGCT 8 cut(s) 95, 348, 357, 407, 447, 470, 552, 573
AluI AGCT 8 cut(s) 95, 348, 357, 407, 447, 470, 552, 573
Alw21I GWGCWC 1 cut(s) 554
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 1 cut(s) 854
AlwNI CAGNNNCTG 2 cut(s) 470, 1166
Aor13HI TCCGGA 1 cut(s) 285
AoxI GGCC 4 cut(s) 499, 753, 864, 994
ApeKI GCWGC 4 cut(s) 362, 464, 467, 523
ApoI RAATTY 3 cut(s) 281, 430, 1006
AsuC2I CCSGG 1 cut(s) 318
AsuHPI GGTGA 3 cut(s) 289, 403, 950
AsuNHI GCTAGC 1 cut(s) 443
BalI TGGCCA 1 cut(s) 755
BanII GRGCYC 1 cut(s) 554
BauI CACGAG 1 cut(s) 618
Bbv12I GWGCWC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 349, 476, 479, 535
BccI CCATC 2 cut(s) 118, 884
BceAI ACGGC 2 cut(s) 842, 879
BciT130I CCWGG 3 cut(s) 641, 1029, 1110
BcnI CCSGG 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 283
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 2 cut(s) 381, 444
BfmI CTRYAG 2 cut(s) 366, 740
BfrI CTTAAG 1 cut(s) 322
BglI GCCNNNNNGGC 1 cut(s) 863
BisI GCNGC 6 cut(s) 156, 363, 465, 468, 524, 994
BlsI GCNGC 6 cut(s) 157, 364, 466, 469, 525, 995
Bme1390I CCNGG 4 cut(s) 318, 641, 1029, 1110
BmiI GGNNCC 1 cut(s) 895
BmrFI CCNGG 4 cut(s) 318, 641, 1029, 1110
BmsI GCATC 4 cut(s) 189, 430, 964, 1155
BmtI GCTAGC 1 cut(s) 447
BpmI CTGGAG 5 cut(s) 412, 1011, 1038, 1065, 1092
Bpu10I CCTNAGC 4 cut(s) 1041, 1068, 1095, 1122
BpuMI CCSGG 1 cut(s) 318
BsaAI YACGTR 2 cut(s) 205, 595
BsaJI CCNNGG 2 cut(s) 48, 316
BsaWI WCCGGW 2 cut(s) 285, 299
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 358, 640, 817, 903
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 3 cut(s) 395, 706, 764
Bse3DI GCAATG 2 cut(s) 125, 226
BseAI TCCGGA 1 cut(s) 285
BseBI CCWGG 3 cut(s) 641, 1029, 1110
BseDI CCNNGG 2 cut(s) 48, 316
BseGI GGATG 2 cut(s) 74, 129
BseLI CCNNNNNNNGG 5 cut(s) 154, 358, 640, 817, 903
BseMI GCAATG 2 cut(s) 125, 226
BseNI ACTGG 3 cut(s) 395, 706, 764
BseXI GCAGC 4 cut(s) 349, 476, 479, 535
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 4 cut(s) 501, 755, 866, 996
BsiHKAI GWGCWC 1 cut(s) 554
BsiSI CCGG 5 cut(s) 286, 300, 318, 498, 828
BslFI GGGAC 5 cut(s) 67, 701, 705, 853, 892
BslI CCNNNNNNNGG 5 cut(s) 154, 358, 640, 817, 903
BsmAI GTCTC 1 cut(s) 283
BsmFI GGGAC 5 cut(s) 67, 701, 705, 853, 892
BsnI GGCC 4 cut(s) 501, 755, 866, 996
Bsp1286I GDGCHC 1 cut(s) 554
Bsp13I TCCGGA 1 cut(s) 285
Bsp143I GATC 4 cut(s) 424, 696, 736, 846
BspACI CCGC 5 cut(s) 155, 582, 689, 811, 993
BspANI GGCC 4 cut(s) 501, 755, 866, 996
BspEI TCCGGA 1 cut(s) 285
BspFNI CGCG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 895
BspOI GCTAGC 1 cut(s) 447
BspPI GGATC 1 cut(s) 854
BspTI CTTAAG 1 cut(s) 322
BsrDI GCAATG 2 cut(s) 125, 226
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 3 cut(s) 395, 706, 764
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 48, 316
BssMI GATC 4 cut(s) 424, 696, 736, 846
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 3 cut(s) 641, 1029, 1110
Bst4CI ACNGT 4 cut(s) 460, 566, 971, 1168
BstAFI CTTAAG 1 cut(s) 322
BstBAI YACGTR 2 cut(s) 205, 595
BstC8I GCNNGC 2 cut(s) 445, 687
BstDEI CTNAG 6 cut(s) 574, 962, 1041, 1068, 1095, 1122
BstF5I GGATG 2 cut(s) 74, 129
BstFNI CGCG 1 cut(s) 141
BstKTI GATC 4 cut(s) 427, 699, 739, 849
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 4 cut(s) 424, 696, 736, 846
BstMWI GCNNNNNNNGC 8 cut(s) 68, 101, 354, 444, 449, 558, 623, 863
BstNI CCWGG 3 cut(s) 641, 1029, 1110
BstNSI RCATGY 2 cut(s) 663, 689
BstSCI CCNGG 4 cut(s) 316, 639, 1027, 1108
BstSFI CTRYAG 2 cut(s) 366, 740
BstSNI TACGTA 1 cut(s) 595
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 4 cut(s) 349, 476, 479, 535
BsuRI GGCC 4 cut(s) 501, 755, 866, 996
BtgZI GCGATG 1 cut(s) 453
BtsCI GGATG 2 cut(s) 74, 129
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 3 cut(s) 699, 820, 976
Cac8I GCNNGC 2 cut(s) 445, 687
CaiI CAGNNNCTG 2 cut(s) 470, 1166
Cfr10I RCCGGY 1 cut(s) 497
CseI GACGC 1 cut(s) 147
Csp6I GTAC 2 cut(s) 599, 808
CspCI CAANNNNNGTGG 2 cut(s) 366, 401
CviAII CATG 8 cut(s) 68, 113, 435, 652, 660, 686, 757, 921
CviQI GTAC 2 cut(s) 599, 808
DdeI CTNAG 6 cut(s) 574, 962, 1041, 1068, 1095, 1122
DpnI GATC 4 cut(s) 426, 698, 738, 848
DpnII GATC 4 cut(s) 424, 696, 736, 846
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 3 cut(s) 499, 753, 864
EciI GGCGGA 1 cut(s) 571
Ecl136II GAGCTC 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 595
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 554
Eco53kI GAGCTC 1 cut(s) 552
Eco57I CTGAAG 2 cut(s) 169, 906
EcoICRI GAGCTC 1 cut(s) 552
EcoRI GAATTC 2 cut(s) 281, 1006
EcoRII CCWGG 3 cut(s) 639, 1027, 1108
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 8 cut(s) 71, 116, 438, 655, 663, 689, 760, 924
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 5 cut(s) 67, 701, 705, 853, 892
FatI CATG 8 cut(s) 67, 112, 434, 651, 659, 685, 756, 920
FauI CCCGC 1 cut(s) 682
Fnu4HI GCNGC 6 cut(s) 156, 363, 465, 468, 524, 994
FokI GGATG 2 cut(s) 61, 136
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 6 cut(s) 156, 363, 465, 468, 524, 994
FspBI CTAG 2 cut(s) 381, 444
GluI GCNGC 6 cut(s) 156, 363, 465, 468, 524, 994
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 5 cut(s) 412, 1011, 1038, 1065, 1092
HaeIII GGCC 4 cut(s) 501, 755, 866, 996
HapII CCGG 5 cut(s) 286, 300, 318, 498, 828
HgaI GACGC 1 cut(s) 147
Hin1II CATG 8 cut(s) 71, 116, 438, 655, 663, 689, 760, 924
HindIII AAGCTT 2 cut(s) 93, 355
HinfI GANTC 5 cut(s) 303, 578, 612, 714, 958
HpaII CCGG 5 cut(s) 286, 300, 318, 498, 828
HphI GGTGA 3 cut(s) 289, 403, 950
Hpy166II GTNNAC 3 cut(s) 145, 388, 535
Hpy188I TCNGA 7 cut(s) 265, 520, 549, 1005, 1020, 1151, 1175
Hpy188III TCNNGA 4 cut(s) 187, 286, 620, 723
Hpy8I GTNNAC 3 cut(s) 145, 388, 535
HpyAV CCTTC 5 cut(s) 90, 157, 470, 729, 739
HpyCH4III ACNGT 4 cut(s) 460, 566, 971, 1168
HpyCH4IV ACGT 3 cut(s) 204, 332, 594
HpyCH4V TGCA 4 cut(s) 540, 561, 760, 1146
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 101, 354, 444, 449, 558, 623, 863
HpyF3I CTNAG 6 cut(s) 574, 962, 1041, 1068, 1095, 1122
HpySE526I ACGT 3 cut(s) 204, 332, 594
Hsp92II CATG 8 cut(s) 71, 116, 438, 655, 663, 689, 760, 924
Kpn2I TCCGGA 1 cut(s) 285
Kzo9I GATC 4 cut(s) 424, 696, 736, 846
LmnI GCTCC 4 cut(s) 1030, 1057, 1084, 1111
Lsp1109I GCAGC 4 cut(s) 349, 476, 479, 535
LweI GCATC 4 cut(s) 189, 430, 964, 1155
MaeI CTAG 2 cut(s) 381, 444
MaeII ACGT 3 cut(s) 204, 332, 594
MaeIII GTNAC 3 cut(s) 409, 510, 859
MalI GATC 4 cut(s) 426, 698, 738, 848
MboI GATC 4 cut(s) 424, 696, 736, 846
MboII GAAGA 4 cut(s) 175, 202, 899, 925
MfeI CAATTG 2 cut(s) 503, 832
MhlI GDGCHC 1 cut(s) 554
MlsI TGGCCA 1 cut(s) 755
MluCI AATT 6 cut(s) 281, 340, 430, 503, 832, 1006
MluI ACGCGT 1 cut(s) 139
MluNI TGGCCA 1 cut(s) 755
MlyI GAGTC 2 cut(s) 606, 723
MmeI TCCRAC 2 cut(s) 401, 1043
MnlI CCTC 7 cut(s) 271, 346, 387, 602, 922, 948, 976
Mox20I TGGCCA 1 cut(s) 755
MroI TCCGGA 1 cut(s) 285
MscI TGGCCA 1 cut(s) 755
MseI TTAA 3 cut(s) 18, 323, 908
Msp20I TGGCCA 1 cut(s) 755
MspA1I CMGCKG 1 cut(s) 470
MspCI CTTAAG 1 cut(s) 322
MspI CCGG 5 cut(s) 286, 300, 318, 498, 828
MspR9I CCNGG 4 cut(s) 318, 641, 1029, 1110
MunI CAATTG 2 cut(s) 503, 832
MvaI CCWGG 3 cut(s) 641, 1029, 1110
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 8 cut(s) 68, 101, 354, 444, 449, 558, 623, 863
NciI CCSGG 1 cut(s) 318
NdeII GATC 4 cut(s) 424, 696, 736, 846
NheI GCTAGC 1 cut(s) 443
NlaIII CATG 8 cut(s) 71, 116, 438, 655, 663, 689, 760, 924
NlaIV GGNNCC 1 cut(s) 895
NmuCI GTSAC 3 cut(s) 409, 510, 859
NspI RCATGY 2 cut(s) 663, 689
PaeI GCATGC 1 cut(s) 689
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PfeI GAWTC 3 cut(s) 303, 578, 958
PflMI CCANNNNNTGG 1 cut(s) 358
PfoI TCCNGGA 2 cut(s) 1027, 1108
PkrI GCNGC 6 cut(s) 157, 364, 466, 469, 525, 995
PleI GAGTC 2 cut(s) 606, 722
PpsI GAGTC 2 cut(s) 606, 722
Ppu21I YACGTR 2 cut(s) 205, 595
Psp124BI GAGCTC 1 cut(s) 554
Psp6I CCWGG 3 cut(s) 639, 1027, 1108
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 3 cut(s) 639, 1027, 1108
PspN4I GGNNCC 1 cut(s) 895
PsrI GAACNNNNNNTAC 2 cut(s) 649, 681
PstNI CAGNNNCTG 2 cut(s) 470, 1166
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 2 cut(s) 600, 809
RsaNI GTAC 2 cut(s) 599, 808
SacI GAGCTC 1 cut(s) 554
SaqAI TTAA 3 cut(s) 18, 323, 908
SatI GCNGC 6 cut(s) 156, 363, 465, 468, 524, 994
Sau3AI GATC 4 cut(s) 424, 696, 736, 846
SchI GAGTC 2 cut(s) 606, 723
ScrFI CCNGG 4 cut(s) 318, 641, 1029, 1110
SduI GDGCHC 1 cut(s) 554
SfaNI GCATC 4 cut(s) 189, 430, 964, 1155
SfcI CTRYAG 2 cut(s) 366, 740
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
SnaBI TACGTA 1 cut(s) 595
SpeI ACTAGT 1 cut(s) 380
SphI GCATGC 1 cut(s) 689
Sse9I AATT 6 cut(s) 281, 340, 430, 503, 832, 1006
SsiI CCGC 5 cut(s) 155, 582, 689, 811, 993
SspMI CTAG 2 cut(s) 381, 444
SstI GAGCTC 1 cut(s) 554
StyD4I CCNGG 4 cut(s) 316, 639, 1027, 1108
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 4 cut(s) 460, 566, 971, 1168
TaiI ACGT 3 cut(s) 207, 335, 597
TaqI TCGA 2 cut(s) 198, 849
TasI AATT 6 cut(s) 281, 340, 430, 503, 832, 1006
TatI WGTACW 1 cut(s) 598
TauI GCSGC 2 cut(s) 158, 996
TfiI GAWTC 3 cut(s) 303, 578, 958
Tru1I TTAA 3 cut(s) 18, 323, 908
Tru9I TTAA 3 cut(s) 18, 323, 908
TscAI CASTG 3 cut(s) 706, 820, 976
TseFI GTSAC 3 cut(s) 409, 510, 859
TseI GCWGC 4 cut(s) 362, 464, 467, 523
Tsp45I GTSAC 3 cut(s) 409, 510, 859
TspDTI ATGAA 6 cut(s) 31, 105, 237, 423, 723, 926
TspGWI ACGGA 2 cut(s) 150, 621
TspRI CASTG 3 cut(s) 706, 820, 976
Van91I CCANNNNNTGG 1 cut(s) 358
Vha464I CTTAAG 1 cut(s) 322
XapI RAATTY 3 cut(s) 281, 430, 1006
XceI RCATGY 2 cut(s) 663, 689
XspI CTAG 2 cut(s) 381, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.