MD16G1032300.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000341
Physical Location & Seq
Reverse (-)
2317839 .. 2319049
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1032300.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCTTTTGAAAGAAACATTTTAATTGTTGCCATACTCATCACCTTGGGGACCTTGGCATCTCAAGCCACGTCCCGCACGTTGTACGAAGCTTCCATTGCTGCAAAACATGAGCAATGGATGGAAAAGTTTGGGCGCGTTTACCAGGACAGCGCAGAGAAGGAAAGGCGTTTCCACATATTCAAGAACAATGTGGAATTCGTGGAGAAATTCAACAGTGAAGGGAAGAAAACATACGAGCTAGGCGTCAATCGATTTTCTGATATGACCTATGAAGAATTTCTCAGTCATCATACTGGATACAAGAAGCCCACTGAATCAACCTCATCCGCAGATGCATCTTTTACGTACAAAAACCTAAGCCTGACGGATGTTCCCCCAAGCATGGACTGGAGGGAGCAAGGAGCTGTTACTCCTGTAAAAGATCAAGGGCGCTGTGGTTGTTGTTGGGCATTTTCTGCAGTGGCAGCAGTCGAAGGGATTACTGAAATCAAAACTGGCAAATTGATCTCACTATCTGAGCAACAACTTGTGAGCTGTGATACAAGTAACTCAGGCTGCAATGGTGGTAACATGGTTAAAGCCTTTGCATACATTCAACGAAACGGAGGAATTGCCAGTGAAGCAAATTACCCATACCAGAGTTCGGATGGAAGATCAGGAACATGTGATAAGAACAGGGCAAATGAGGCTGCTGCCAAGATAACTAATTATGCAAGGGTACCTCGCAACAGTGAAGCTGATATAAACAAGGCTGTGTCCATGCAACCAATCTCCGTTGCCATCGTTGCCTCACAAGCCTTTCAAGCCTATAAAGGCGGGCTGTTCACTGATGATAATTGTGGGACAAATTTAAACCACGCTGTTACCATCATTGGGTATGGGACAACGGAAGATGACACCCCCTATTGGTTAATCAAGAACTCATGGGGCACGTCGTGGGGTGAGAATGGCTACATGAAGATTCTTAGATACGCCGATGCCCCAGAAGGTGTTTGTGGCCTCGCTATGGATGCTTCCTATCCAATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

345

Amino Acids

37.85

Weight (kDa)

5.67

Isoelectric Point (pI)

31.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 8.3e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 343 2.1e-81 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 721
AccB1I GGYRCC 1 cut(s) 721
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 76, 330, 819
AcsI RAATTY 4 cut(s) 197, 209, 278, 850
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 939
AfaI GTAC 3 cut(s) 86, 350, 723
AfiI CCNNNNNNNGG 5 cut(s) 385, 646, 876, 909, 1009
AflIII ACRYGT 1 cut(s) 665
AgsI TTSAA 5 cut(s) 11, 184, 214, 599, 806
AjiI CACGTC 2 cut(s) 72, 936
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 5 cut(s) 92, 241, 407, 537, 740
AluI AGCT 5 cut(s) 92, 241, 407, 537, 740
AoxI GGCC 1 cut(s) 1000
ApeKI GCWGC 5 cut(s) 101, 467, 558, 692, 695
ApoI RAATTY 4 cut(s) 197, 209, 278, 850
Asp700I GAANNNNTTC 1 cut(s) 279
Asp718I GGTACC 1 cut(s) 721
AspLEI GCGC 3 cut(s) 138, 155, 435
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 34, 956
AvaII GGWCC 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 935
BanI GGYRCC 1 cut(s) 721
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbvI GCAGC 5 cut(s) 88, 479, 545, 679, 682
BccI CCATC 4 cut(s) 115, 644, 791, 878
BciT130I CCWGG 1 cut(s) 146
BciVI GTATCC 1 cut(s) 293
BfaI CTAG 1 cut(s) 242
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 436
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 5 cut(s) 102, 468, 559, 693, 696
BlsI GCNGC 5 cut(s) 103, 469, 560, 694, 697
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 2 cut(s) 72, 936
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 2 cut(s) 52, 723
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 5 cut(s) 68, 325, 347, 970, 1003
BpmI CTGGAG 1 cut(s) 412
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 48
Bsa29I ATCGAT 1 cut(s) 253
BsaAI YACGTR 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 45, 54
Bsc4I CCNNNNNNNGG 5 cut(s) 385, 646, 876, 909, 1009
Bse1I ACTGG 4 cut(s) 301, 395, 502, 618
Bse3DI GCAATG 3 cut(s) 96, 122, 568
BseBI CCWGG 1 cut(s) 146
BseCI ATCGAT 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 45, 54
BseGI GGATG 5 cut(s) 126, 326, 376, 655, 1018
BseLI CCNNNNNNNGG 5 cut(s) 385, 646, 876, 909, 1009
BseMI GCAATG 3 cut(s) 96, 122, 568
BseMII CTCAG 3 cut(s) 298, 510, 567
BseNI ACTGG 4 cut(s) 301, 395, 502, 618
BseSI GKGCMC 1 cut(s) 935
BseXI GCAGC 5 cut(s) 88, 479, 545, 679, 682
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 1002
BshNI GGYRCC 1 cut(s) 721
BshVI ATCGAT 1 cut(s) 253
BslFI GGGAC 4 cut(s) 58, 64, 859, 898
BslI CCNNNNNNNGG 5 cut(s) 385, 646, 876, 909, 1009
BsmFI GGGAC 4 cut(s) 58, 64, 859, 898
BsnI GGCC 1 cut(s) 1002
Bsp1286I GDGCHC 1 cut(s) 935
Bsp143I GATC 3 cut(s) 424, 507, 656
BspACI CCGC 3 cut(s) 76, 330, 819
BspANI GGCC 1 cut(s) 1002
BspCNI CTCAG 3 cut(s) 297, 511, 566
BspDI ATCGAT 1 cut(s) 253
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 52, 723
BspMAI CTGCAG 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 721
BsrDI GCAATG 3 cut(s) 96, 122, 568
BsrI ACTGG 4 cut(s) 301, 395, 502, 618
BssECI CCNNGG 2 cut(s) 45, 54
BssMI GATC 3 cut(s) 424, 507, 656
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 45, 54
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 218, 734
BstACI GRCGYC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBAI YACGTR 1 cut(s) 348
BstC8I GCNNGC 1 cut(s) 821
BstDEI CTNAG 5 cut(s) 284, 359, 519, 553, 968
BstF5I GGATG 5 cut(s) 126, 326, 376, 655, 1018
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 3 cut(s) 138, 155, 435
BstKTI GATC 3 cut(s) 427, 510, 659
BstMBI GATC 3 cut(s) 424, 507, 656
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 669
BstSCI CCNGG 1 cut(s) 144
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 935
BstSNI TACGTA 1 cut(s) 348
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 5 cut(s) 88, 479, 545, 679, 682
Bsu15I ATCGAT 1 cut(s) 253
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 1002
BsuTUI ATCGAT 1 cut(s) 253
BtrI CACGTC 2 cut(s) 72, 936
BtsCI GGATG 5 cut(s) 126, 326, 376, 655, 1018
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 6 cut(s) 223, 312, 468, 625, 739, 828
Cac8I GCNNGC 1 cut(s) 821
CfoI GCGC 3 cut(s) 138, 155, 435
Cfr13I GGNCC 1 cut(s) 51
ClaI ATCGAT 1 cut(s) 253
CseI GACGC 1 cut(s) 235
Csp6I GTAC 3 cut(s) 85, 349, 722
CviAII CATG 7 cut(s) 110, 385, 574, 666, 763, 927, 958
CviQI GTAC 3 cut(s) 85, 349, 722
DdeI CTNAG 5 cut(s) 284, 359, 519, 553, 968
DpnI GATC 3 cut(s) 426, 509, 658
DpnII GATC 3 cut(s) 424, 507, 656
DraI TTTAAA 1 cut(s) 855
DraIII CACNNNGTG 1 cut(s) 939
Eco105I TACGTA 1 cut(s) 348
Eco130I CCWWGG 2 cut(s) 45, 54
Eco47I GGWCC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 45, 54
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 2 cut(s) 45, 54
FaeI CATG 7 cut(s) 113, 388, 577, 669, 766, 930, 961
FaqI GGGAC 4 cut(s) 58, 64, 859, 898
FatI CATG 7 cut(s) 109, 384, 573, 665, 762, 926, 957
FauI CCCGC 2 cut(s) 83, 812
Fnu4HI GCNGC 5 cut(s) 102, 468, 559, 693, 696
FokI GGATG 5 cut(s) 133, 313, 383, 662, 1025
Fsp4HI GCNGC 5 cut(s) 102, 468, 559, 693, 696
FspBI CTAG 1 cut(s) 242
GlaI GCGC 3 cut(s) 137, 154, 434
GluI GCNGC 5 cut(s) 102, 468, 559, 693, 696
GsuI CTGGAG 1 cut(s) 412
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 1 cut(s) 1002
HgaI GACGC 1 cut(s) 235
HhaI GCGC 3 cut(s) 138, 155, 435
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 7 cut(s) 113, 388, 577, 669, 766, 930, 961
Hin6I GCGC 3 cut(s) 136, 153, 433
HinP1I GCGC 3 cut(s) 136, 153, 433
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 317, 964
HphI GGTGA 2 cut(s) 34, 956
Hpy166II GTNNAC 2 cut(s) 142, 828
Hpy188I TCNGA 3 cut(s) 262, 520, 649
Hpy188III TCNNGA 3 cut(s) 184, 660, 919
Hpy8I GTNNAC 2 cut(s) 142, 828
Hpy99I CGWCG 1 cut(s) 940
HpyAV CCTTC 4 cut(s) 154, 215, 470, 983
HpyCH4III ACNGT 2 cut(s) 218, 734
HpyCH4IV ACGT 4 cut(s) 71, 80, 347, 935
HpyCH4V TGCA 7 cut(s) 104, 338, 461, 561, 590, 716, 766
HpyF3I CTNAG 5 cut(s) 284, 359, 519, 553, 968
HpySE526I ACGT 4 cut(s) 71, 80, 347, 935
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 7 cut(s) 113, 388, 577, 669, 766, 930, 961
HspAI GCGC 3 cut(s) 136, 153, 433
KpnI GGTACC 1 cut(s) 725
Kzo9I GATC 3 cut(s) 424, 507, 656
LmnI GCTCC 2 cut(s) 397, 404
Lsp1109I GCAGC 5 cut(s) 88, 479, 545, 679, 682
LweI GCATC 5 cut(s) 68, 325, 347, 970, 1003
MaeI CTAG 1 cut(s) 242
MaeII ACGT 4 cut(s) 71, 80, 347, 935
MaeIII GTNAC 4 cut(s) 409, 548, 569, 865
MalI GATC 3 cut(s) 426, 509, 658
MboI GATC 3 cut(s) 424, 507, 656
MboII GAAGA 5 cut(s) 238, 287, 666, 905, 973
MhlI GDGCHC 1 cut(s) 935
MnlI CCTC 7 cut(s) 334, 387, 602, 682, 735, 802, 1013
Mph1103I ATGCAT 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 4 cut(s) 23, 579, 854, 914
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 138
NdeII GATC 3 cut(s) 424, 507, 656
NlaIII CATG 7 cut(s) 113, 388, 577, 669, 766, 930, 961
NlaIV GGNNCC 2 cut(s) 52, 723
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 669
PciI ACATGT 1 cut(s) 665
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 243
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 317, 964
PkrI GCNGC 5 cut(s) 103, 469, 560, 694, 697
Ppu21I YACGTR 1 cut(s) 348
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 2 cut(s) 52, 723
PspPI GGNCC 1 cut(s) 51
PspPPI RGGWCCY 1 cut(s) 51
PstI CTGCAG 1 cut(s) 463
RsaI GTAC 3 cut(s) 86, 350, 723
RsaNI GTAC 3 cut(s) 85, 349, 722
SaqAI TTAA 4 cut(s) 23, 579, 854, 914
SatI GCNGC 5 cut(s) 102, 468, 559, 693, 696
Sau3AI GATC 3 cut(s) 424, 507, 656
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 935
SfaNI GCATC 5 cut(s) 68, 325, 347, 970, 1003
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
SnaBI TACGTA 1 cut(s) 348
SsiI CCGC 3 cut(s) 76, 330, 819
SspMI CTAG 1 cut(s) 242
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 2 cut(s) 45, 54
TaaI ACNGT 2 cut(s) 218, 734
TaiI ACGT 4 cut(s) 74, 83, 350, 938
TaqI TCGA 2 cut(s) 253, 474
TfiI GAWTC 2 cut(s) 317, 964
Tru1I TTAA 4 cut(s) 23, 579, 854, 914
Tru9I TTAA 4 cut(s) 23, 579, 854, 914
TscAI CASTG 6 cut(s) 223, 319, 468, 625, 739, 835
TseI GCWGC 5 cut(s) 101, 467, 558, 692, 695
TspDTI ATGAA 2 cut(s) 288, 974
TspGWI ACGGA 4 cut(s) 383, 621, 766, 905
TspRI CASTG 6 cut(s) 223, 319, 468, 625, 739, 835
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 4 cut(s) 197, 209, 278, 850
XceI RCATGY 1 cut(s) 669
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 647
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 1 cut(s) 242
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.