MD10G1282200.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000341
Physical Location & Seq
Forward (+)
37293572 .. 37294723
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1282200.v1.1.491

Sequence Viewer

Length: 762 bp
ATGACCTATGACGAATTCCTCGGGCATTATACAGGATACAAGCAGCCCACTGAATCAACCTCATCCGCAGATGCATCATTTACGTACGAAAACCTAAGCCCGACAGACGTTCCCCAAAGCATTGACTGGAGGGAGAATGGAGCTGTTACTCCAGTAAAAGATCAAGGGCGCTGTGGTTGTTGCTGGGCATTTTCTGCAGTGACAGCAGTCGAAGGGATTATCCAAATCAAAACTGACAAATTGATCTCACTGTCTGAGCAACAACTTGTGAACTGTGATACAGATAACAATGGCTGCCAAGGTGGTTGGATGGTTAATGCCTTTGCATACATTCAACGAAACAGAGGAATTACCAGTGAAGAAAATTACCCATACCAGAGTCTAGAAGGAACATGTGATACGAACAAGGAAAATGAGGCTGCTGCCCAGATAACTAGTTATGCAGAGGTACCTCCCAACAGTGAAACCGATCTACTAAAGGCTGTGTCCATGCAACCAGTCTCCGTTTCCATCCATGCCTCACCAGCCTTTCAGCATTATTCAGGTGGGGTGTTCACCGGTGATGATTGTGGGACAGAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCACCCCCCATTGGTTAATCAAGAATTCATGGTCCGATACGTGGGGTGAGAATGGCTACATGAAGATTCTTAGAAACGCTGATGCCCCAGGTGGTGTTTGTGGCCTTGCTATAAGACCTTTCTATCCGACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

254

Amino Acids

27.62

Weight (kDa)

4.46

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 37 - 251 6.9e-82 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 448
AccB1I GGYRCC 2 cut(s) 448, 626
AciI CCGC 1 cut(s) 66
AcsI RAATTY 2 cut(s) 14, 649
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 2 cut(s) 86, 450
AfiI CCNNNNNNNGG 3 cut(s) 603, 636, 666
AflIII ACRYGT 1 cut(s) 392
AgeI ACCGGT 1 cut(s) 557
AgsI TTSAA 2 cut(s) 335, 583
AhlI ACTAGT 1 cut(s) 434
AjnI CCWGG 1 cut(s) 712
AluBI AGCT 1 cut(s) 143
AluI AGCT 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 505
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 1 cut(s) 727
ApeKI GCWGC 4 cut(s) 43, 294, 419, 422
ApoI RAATTY 2 cut(s) 14, 649
AsiGI ACCGGT 1 cut(s) 557
Asp718I GGTACC 1 cut(s) 448
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 657
AsuHPI GGTGA 4 cut(s) 513, 547, 572, 683
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 657
BanI GGYRCC 2 cut(s) 448, 626
BbvI GCAGC 4 cut(s) 55, 281, 406, 409
BccI CCATC 4 cut(s) 304, 518, 605, 617
BciT130I CCWGG 1 cut(s) 714
BciVI GTATCC 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 505
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 2 cut(s) 383, 435
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 172
BfuI GTATCC 1 cut(s) 29
BisI GCNGC 4 cut(s) 44, 295, 420, 423
BlsI GCNGC 4 cut(s) 45, 296, 421, 424
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 1 cut(s) 657
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 657
BmiI GGNNCC 2 cut(s) 450, 628
BmrFI CCNGG 1 cut(s) 714
BmsI GCATC 3 cut(s) 61, 83, 697
BoxI GACNNNNGTC 1 cut(s) 206
BpmI CTGGAG 2 cut(s) 135, 148
Bpu10I CCTNAGC 1 cut(s) 95
BsaAI YACGTR 2 cut(s) 84, 666
BsaJI CCNNGG 3 cut(s) 19, 298, 712
BsaWI WCCGGW 1 cut(s) 557
Bsc4I CCNNNNNNNGG 3 cut(s) 603, 636, 666
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 4 cut(s) 131, 152, 354, 497
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 3 cut(s) 19, 298, 712
BseGI GGATG 3 cut(s) 62, 315, 510
BseLI CCNNNNNNNGG 3 cut(s) 603, 636, 666
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 4 cut(s) 131, 152, 354, 497
BseXI GCAGC 4 cut(s) 55, 281, 406, 409
BseYI CCCAGC 1 cut(s) 183
BshFI GGCC 1 cut(s) 729
BshNI GGYRCC 2 cut(s) 448, 626
BshTI ACCGGT 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 558
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 2 cut(s) 586, 625
BslI CCNNNNNNNGG 3 cut(s) 603, 636, 666
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 2 cut(s) 586, 625
BsnI GGCC 1 cut(s) 729
BsoBI CYCGRG 1 cut(s) 20
Bsp143I GATC 3 cut(s) 160, 243, 469
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 729
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 450, 628
BspMAI CTGCAG 1 cut(s) 199
BspT107I GGYRCC 2 cut(s) 448, 626
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 4 cut(s) 131, 152, 354, 497
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 3 cut(s) 19, 298, 712
BssMI GATC 3 cut(s) 160, 243, 469
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 3 cut(s) 252, 275, 461
BstAPI GCANNNNNTGC 1 cut(s) 194
BstBAI YACGTR 2 cut(s) 84, 666
BstDEI CTNAG 3 cut(s) 95, 255, 695
BstF5I GGATG 3 cut(s) 62, 315, 510
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 3 cut(s) 163, 246, 472
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 3 cut(s) 160, 243, 469
BstMWI GCNNNNNNNGC 3 cut(s) 194, 203, 524
BstNI CCWGG 1 cut(s) 714
BstNSI RCATGY 1 cut(s) 396
BstPAI GACNNNNGTC 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 712
BstSFI CTRYAG 1 cut(s) 195
BstSNI TACGTA 1 cut(s) 84
BstV1I GCAGC 4 cut(s) 55, 281, 406, 409
BsuI GTATCC 1 cut(s) 29
BsuRI GGCC 1 cut(s) 729
BtsCI GGATG 3 cut(s) 62, 315, 510
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 5 cut(s) 48, 204, 248, 361, 466
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 1 cut(s) 657
Csp6I GTAC 2 cut(s) 85, 449
CspAI ACCGGT 1 cut(s) 557
CviAII CATG 5 cut(s) 393, 490, 515, 654, 685
CviJI RGCY 9 cut(s) 46, 99, 143, 294, 419, 482, 527, 681, 729
CviKI_1 RGCY 9 cut(s) 46, 99, 143, 294, 419, 482, 527, 681, 729
CviQI GTAC 2 cut(s) 85, 449
DdeI CTNAG 3 cut(s) 95, 255, 695
DpnI GATC 3 cut(s) 162, 245, 471
DpnII GATC 3 cut(s) 160, 243, 469
Eco105I TACGTA 1 cut(s) 84
Eco130I CCWWGG 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 657
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 20
EcoRI GAATTC 2 cut(s) 14, 649
EcoRII CCWGG 1 cut(s) 712
EcoT14I CCWWGG 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 76
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 5 cut(s) 396, 493, 518, 657, 688
FaqI GGGAC 2 cut(s) 586, 625
FatI CATG 5 cut(s) 392, 489, 514, 653, 684
Fnu4HI GCNGC 4 cut(s) 44, 295, 420, 423
FokI GGATG 3 cut(s) 49, 322, 497
Fsp4HI GCNGC 4 cut(s) 44, 295, 420, 423
FspBI CTAG 2 cut(s) 383, 435
GlaI GCGC 1 cut(s) 170
GluI GCNGC 4 cut(s) 44, 295, 420, 423
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 2 cut(s) 135, 148
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 1 cut(s) 729
HapII CCGG 1 cut(s) 558
HhaI GCGC 1 cut(s) 171
Hin1II CATG 5 cut(s) 396, 493, 518, 657, 688
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HinfI GANTC 3 cut(s) 53, 379, 691
HpaII CCGG 1 cut(s) 558
HphI GGTGA 4 cut(s) 513, 547, 572, 683
Hpy166II GTNNAC 2 cut(s) 271, 555
Hpy188I TCNGA 3 cut(s) 256, 661, 753
Hpy188III TCNNGA 2 cut(s) 383, 646
Hpy8I GTNNAC 2 cut(s) 271, 555
HpyAV CCTTC 2 cut(s) 206, 380
HpyCH4III ACNGT 3 cut(s) 252, 275, 461
HpyCH4IV ACGT 3 cut(s) 83, 108, 665
HpyCH4V TGCA 5 cut(s) 74, 197, 326, 443, 493
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 203, 524
HpyF3I CTNAG 3 cut(s) 95, 255, 695
HpySE526I ACGT 3 cut(s) 83, 108, 665
Hsp92II CATG 5 cut(s) 396, 493, 518, 657, 688
HspAI GCGC 1 cut(s) 169
KpnI GGTACC 1 cut(s) 452
Kzo9I GATC 3 cut(s) 160, 243, 469
LmnI GCTCC 1 cut(s) 140
Lsp1109I GCAGC 4 cut(s) 55, 281, 406, 409
LweI GCATC 3 cut(s) 61, 83, 697
MaeI CTAG 2 cut(s) 383, 435
MaeII ACGT 3 cut(s) 83, 108, 665
MaeIII GTNAC 3 cut(s) 145, 199, 592
MalI GATC 3 cut(s) 162, 245, 471
MboI GATC 3 cut(s) 160, 243, 469
MboII GAAGA 3 cut(s) 371, 632, 700
MluCI AATT 6 cut(s) 14, 239, 348, 364, 578, 649
MlyI GAGTC 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 287
MnlI CCTC 8 cut(s) 29, 70, 123, 338, 409, 439, 462, 529
Mph1103I ATGCAT 1 cut(s) 76
MseI TTAA 2 cut(s) 315, 641
MspI CCGG 1 cut(s) 558
MspR9I CCNGG 1 cut(s) 714
MvaI CCWGG 1 cut(s) 714
MwoI GCNNNNNNNGC 3 cut(s) 194, 203, 524
NdeII GATC 3 cut(s) 160, 243, 469
NlaIII CATG 5 cut(s) 396, 493, 518, 657, 688
NlaIV GGNNCC 2 cut(s) 450, 628
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 1 cut(s) 396
PciI ACATGT 1 cut(s) 392
PfeI GAWTC 2 cut(s) 53, 691
Pfl23II CGTACG 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 557
PkrI GCNGC 4 cut(s) 45, 296, 421, 424
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
Ppu21I YACGTR 2 cut(s) 84, 666
PscI ACATGT 1 cut(s) 392
PshAI GACNNNNGTC 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 712
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 712
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 450, 628
PspPI GGNCC 1 cut(s) 657
PsrI GAACNNNNNNTAC 2 cut(s) 382, 414
PstI CTGCAG 1 cut(s) 199
RsaI GTAC 2 cut(s) 86, 450
RsaNI GTAC 2 cut(s) 85, 449
SaqAI TTAA 2 cut(s) 315, 641
SatI GCNGC 4 cut(s) 44, 295, 420, 423
Sau3AI GATC 3 cut(s) 160, 243, 469
Sau96I GGNCC 1 cut(s) 657
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 714
SfaNI GCATC 3 cut(s) 61, 83, 697
SfcI CTRYAG 1 cut(s) 195
SgrAI CRCCGGYG 1 cut(s) 557
SinI GGWCC 1 cut(s) 657
SnaBI TACGTA 1 cut(s) 84
SpeI ACTAGT 1 cut(s) 434
Sse9I AATT 6 cut(s) 14, 239, 348, 364, 578, 649
SsiI CCGC 1 cut(s) 66
SspMI CTAG 2 cut(s) 383, 435
StyD4I CCNGG 1 cut(s) 712
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 3 cut(s) 252, 275, 461
TaiI ACGT 3 cut(s) 86, 111, 668
TaqI TCGA 1 cut(s) 210
TasI AATT 6 cut(s) 14, 239, 348, 364, 578, 649
TfiI GAWTC 2 cut(s) 53, 691
Tru1I TTAA 2 cut(s) 315, 641
Tru9I TTAA 2 cut(s) 315, 641
TscAI CASTG 5 cut(s) 55, 204, 255, 361, 466
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 4 cut(s) 43, 294, 419, 422
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 642, 701
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 5 cut(s) 55, 204, 255, 361, 466
VpaK11BI GGWCC 1 cut(s) 657
XapI RAATTY 2 cut(s) 14, 649
XbaI TCTAGA 1 cut(s) 382
XceI RCATGY 1 cut(s) 396
XspI CTAG 2 cut(s) 383, 435
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.