MD10G1281600.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000341
Physical Location & Seq
Forward (+)
37222580 .. 37230491
7912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1281600.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGGCTTTTGAAAGAAACCTTTTGATTGTTACCATACTCATCACCTTGGGGACCTTGGCATCTGAAGCCACGTCCCGCACATTGTACCAAGCTTCCATTGCTGCAAAACATGAGCAGTGGATGAAAAAGCATGGACGCGTTTACCAGGACAGCGACAGTGCGGAGAAGGAAAGGCGTTTCCTGATATTCAAAAACAATATGGAGTTTGTCGAGAAATTCAACAGTGAAGGGAACATGACTTACAGCCTATGCGTCAACCGATTTTCGGATATGAGCTATGACGAATTCCTCAGGTATTATACTGGATACCAGCAGCCCACTGAATCAACCTCATCCGCAGATGCATCATTTACGTACGAAAACCTAAGCCCGACGGATGTTCCCCCAAGCATTGACTGGAGGGACAATGGAGCTGTTACTCCGGTAAAAGATCAAGGGCGCTGTGGTTGTTGCTGGGCATTTTCTGCAGTGGCAGCAGTCGAAGGGATTACCCAAATCAAAACTGGCAAATTGATCTCACTGTCTGAGCAACAGCTTGTGAACTGTGATACAGATAACAACAGGGGCTGCCAAGGTGGTTGGATGGTTGATGCCTTTGCATACATTAAACAAAACGGAGGAATTACCAGTGAAGAAAATTACCCATACCAGAGTTTAGATGGCAGTGAAGGAACATGTGATATGAACAAGGCAAAAGAGGCTGCTGCCCGGATAACTAGTTATGCAGAGGTACCTCCCAACAGTGAAACCGATCTACTCAAGGCTGTGTCCATGCAACCAGTCTCCGTTGCCATCAATATGTCACCAGCCTTTCAGCATTATTCAGGCGGGGTGTTCACCGGTGATGATTGTGGGACATCATTGGACCACGCTGTTACCGTCATTGGGTATGGGACAACTGATGATGGCACCCCCTATTGGTTAATCAAGAATTCATGGTCCGATACGTGGGGTGAGAATGGCTACATGAAGATTCTTAGAAACGCTGAAGCCCCAGGTGGTGTTTGTAGCCTCGCTACGAAAGCTTCCTATCCGACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

348

Amino Acids

38.24

Weight (kDa)

4.77

Isoelectric Point (pI)

35.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 96 2.8e-17 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 127 - 346 3.8e-82 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 730
AccB1I GGYRCC 2 cut(s) 730, 908
AccII CGCG 1 cut(s) 138
AciI CCGC 4 cut(s) 76, 161, 336, 828
AcsI RAATTY 3 cut(s) 215, 284, 931
AcuI CTGAAG 2 cut(s) 84, 1008
AfaI GTAC 3 cut(s) 86, 356, 732
AfiI CCNNNNNNNGG 4 cut(s) 265, 885, 918, 948
AflIII ACRYGT 2 cut(s) 136, 674
AgeI ACCGGT 1 cut(s) 839
AgsI TTSAA 3 cut(s) 11, 190, 220
AhlI ACTAGT 1 cut(s) 716
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 2 cut(s) 144, 994
AluBI AGCT 5 cut(s) 92, 276, 413, 535, 1025
AluI AGCT 5 cut(s) 92, 276, 413, 535, 1025
Alw26I GTCTC 1 cut(s) 787
AlwNI CAGNNNCTG 1 cut(s) 567
ApeKI GCWGC 6 cut(s) 101, 313, 473, 567, 701, 704
ApoI RAATTY 3 cut(s) 215, 284, 931
AsiGI ACCGGT 1 cut(s) 839
Asp718I GGTACC 1 cut(s) 730
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 3 cut(s) 51, 865, 939
AsuC2I CCSGG 1 cut(s) 709
AsuHPI GGTGA 5 cut(s) 34, 795, 829, 854, 965
AvaII GGWCC 3 cut(s) 51, 865, 939
AxyI CCTNAGG 1 cut(s) 290
BanI GGYRCC 2 cut(s) 730, 908
BbvI GCAGC 6 cut(s) 88, 325, 485, 554, 688, 691
BccI CCATC 4 cut(s) 577, 653, 800, 899
BciT130I CCWGG 2 cut(s) 146, 996
BciVI GTATCC 1 cut(s) 299
BcnI CCSGG 1 cut(s) 709
BcoDI GTCTC 1 cut(s) 787
BcuI ACTAGT 1 cut(s) 716
BfaI CTAG 1 cut(s) 717
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 442
BfuI GTATCC 1 cut(s) 299
BisI GCNGC 6 cut(s) 102, 314, 474, 568, 702, 705
BlsI GCNGC 6 cut(s) 103, 315, 475, 569, 703, 706
Bme1390I CCNGG 3 cut(s) 146, 709, 996
Bme18I GGWCC 3 cut(s) 51, 865, 939
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 51, 865, 939
BmiI GGNNCC 3 cut(s) 52, 732, 910
BmrFI CCNGG 3 cut(s) 146, 709, 996
BmsI GCATC 4 cut(s) 68, 331, 353, 580
BpmI CTGGAG 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 1 cut(s) 709
BsaAI YACGTR 2 cut(s) 354, 948
BsaJI CCNNGG 4 cut(s) 45, 54, 571, 994
BsaWI WCCGGW 2 cut(s) 421, 839
Bsc4I CCNNNNNNNGG 4 cut(s) 265, 885, 918, 948
Bse118I RCCGGY 1 cut(s) 839
Bse1I ACTGG 5 cut(s) 307, 401, 508, 627, 779
Bse21I CCTNAGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 2 cut(s) 146, 996
BseDI CCNNGG 4 cut(s) 45, 54, 571, 994
BseGI GGATG 4 cut(s) 126, 332, 382, 588
BseLI CCNNNNNNNGG 4 cut(s) 265, 885, 918, 948
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 304, 516
BseNI ACTGG 5 cut(s) 307, 401, 508, 627, 779
BseXI GCAGC 6 cut(s) 88, 325, 485, 554, 688, 691
BseYI CCCAGC 1 cut(s) 453
Bsh1236I CGCG 1 cut(s) 138
BshNI GGYRCC 2 cut(s) 730, 908
BshTI ACCGGT 1 cut(s) 839
BsiSI CCGG 3 cut(s) 422, 709, 840
BsiWI CGTACG 1 cut(s) 354
BslFI GGGAC 5 cut(s) 58, 64, 416, 868, 907
BslI CCNNNNNNNGG 4 cut(s) 265, 885, 918, 948
BsmAI GTCTC 1 cut(s) 787
BsmFI GGGAC 5 cut(s) 58, 64, 416, 868, 907
Bsp143I GATC 3 cut(s) 430, 513, 751
BspACI CCGC 4 cut(s) 76, 161, 336, 828
BspCNI CTCAG 2 cut(s) 303, 517
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 52, 732, 910
BspMAI CTGCAG 1 cut(s) 469
BspT107I GGYRCC 2 cut(s) 730, 908
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 839
BsrI ACTGG 5 cut(s) 307, 401, 508, 627, 779
BssAI RCCGGY 1 cut(s) 839
BssECI CCNNGG 4 cut(s) 45, 54, 571, 994
BssMI GATC 3 cut(s) 430, 513, 751
BssT1I CCWWGG 3 cut(s) 45, 54, 571
Bst2UI CCWGG 2 cut(s) 146, 996
Bst4CI ACNGT 6 cut(s) 158, 224, 522, 545, 743, 880
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBAI YACGTR 2 cut(s) 354, 948
BstDEI CTNAG 4 cut(s) 290, 365, 525, 977
BstF5I GGATG 4 cut(s) 126, 332, 382, 588
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 3 cut(s) 433, 516, 754
BstMAI GTCTC 1 cut(s) 787
BstMBI GATC 3 cut(s) 430, 513, 751
BstMWI GCNNNNNNNGC 6 cut(s) 65, 98, 464, 473, 698, 1022
BstNI CCWGG 2 cut(s) 146, 996
BstNSI RCATGY 1 cut(s) 678
BstSCI CCNGG 3 cut(s) 144, 707, 994
BstSFI CTRYAG 1 cut(s) 465
BstSNI TACGTA 1 cut(s) 354
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 88, 325, 485, 554, 688, 691
Bsu36I CCTNAGG 1 cut(s) 290
BsuI GTATCC 1 cut(s) 299
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 4 cut(s) 126, 332, 382, 588
BtsI GCAGTG 3 cut(s) 122, 474, 670
BtsIMutI CAGTG 9 cut(s) 122, 163, 229, 318, 474, 518, 634, 670, 748
CaiI CAGNNNCTG 1 cut(s) 567
CfoI GCGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 839
Cfr13I GGNCC 3 cut(s) 51, 865, 939
CseI GACGC 2 cut(s) 144, 241
Csp6I GTAC 3 cut(s) 85, 355, 731
CspAI ACCGGT 1 cut(s) 839
CviAII CATG 7 cut(s) 110, 131, 235, 675, 772, 936, 967
CviQI GTAC 3 cut(s) 85, 355, 731
DdeI CTNAG 4 cut(s) 290, 365, 525, 977
DpnI GATC 3 cut(s) 432, 515, 753
DpnII GATC 3 cut(s) 430, 513, 751
Eco105I TACGTA 1 cut(s) 354
Eco130I CCWWGG 3 cut(s) 45, 54, 571
Eco47I GGWCC 3 cut(s) 51, 865, 939
Eco57I CTGAAG 2 cut(s) 84, 1008
Eco81I CCTNAGG 1 cut(s) 290
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 284, 931
EcoRII CCWGG 2 cut(s) 144, 994
EcoT14I CCWWGG 3 cut(s) 45, 54, 571
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 3 cut(s) 45, 54, 571
FaeI CATG 7 cut(s) 113, 134, 238, 678, 775, 939, 970
FaqI GGGAC 5 cut(s) 58, 64, 416, 868, 907
FatI CATG 7 cut(s) 109, 130, 234, 674, 771, 935, 966
FauI CCCGC 2 cut(s) 83, 821
Fnu4HI GCNGC 6 cut(s) 102, 314, 474, 568, 702, 705
FokI GGATG 4 cut(s) 133, 319, 389, 595
Fsp4HI GCNGC 6 cut(s) 102, 314, 474, 568, 702, 705
FspBI CTAG 1 cut(s) 717
GlaI GCGC 1 cut(s) 440
GluI GCNGC 6 cut(s) 102, 314, 474, 568, 702, 705
GsaI CCCAGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 418
HaeII RGCGCY 1 cut(s) 442
HapII CCGG 3 cut(s) 422, 709, 840
HgaI GACGC 2 cut(s) 144, 241
HhaI GCGC 1 cut(s) 441
Hin1II CATG 7 cut(s) 113, 134, 238, 678, 775, 939, 970
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 2 cut(s) 90, 1023
HinfI GANTC 2 cut(s) 323, 973
HpaII CCGG 3 cut(s) 422, 709, 840
HphI GGTGA 5 cut(s) 34, 795, 829, 854, 965
Hpy166II GTNNAC 4 cut(s) 142, 256, 541, 837
Hpy188I TCNGA 5 cut(s) 64, 268, 526, 943, 1035
Hpy188III TCNNGA 3 cut(s) 181, 211, 928
Hpy8I GTNNAC 4 cut(s) 142, 256, 541, 837
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 4 cut(s) 160, 221, 476, 662
HpyCH4III ACNGT 6 cut(s) 158, 224, 522, 545, 743, 880
HpyCH4IV ACGT 3 cut(s) 71, 353, 947
HpyCH4V TGCA 6 cut(s) 104, 344, 467, 599, 725, 775
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 98, 464, 473, 698, 1022
HpyF3I CTNAG 4 cut(s) 290, 365, 525, 977
HpySE526I ACGT 3 cut(s) 71, 353, 947
Hsp92II CATG 7 cut(s) 113, 134, 238, 678, 775, 939, 970
HspAI GCGC 1 cut(s) 439
KpnI GGTACC 1 cut(s) 734
Kzo9I GATC 3 cut(s) 430, 513, 751
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 6 cut(s) 88, 325, 485, 554, 688, 691
LweI GCATC 4 cut(s) 68, 331, 353, 580
MaeI CTAG 1 cut(s) 717
MaeII ACGT 3 cut(s) 71, 353, 947
MaeIII GTNAC 4 cut(s) 28, 415, 801, 874
MalI GATC 3 cut(s) 432, 515, 753
MboI GATC 3 cut(s) 430, 513, 751
MboII GAAGA 2 cut(s) 644, 982
MluCI AATT 6 cut(s) 215, 284, 509, 621, 637, 931
MluI ACGCGT 1 cut(s) 136
MmeI TCCRAC 1 cut(s) 560
MnlI CCTC 8 cut(s) 299, 340, 393, 611, 691, 721, 744, 1022
Mph1103I ATGCAT 1 cut(s) 346
MseI TTAA 2 cut(s) 606, 923
MslI CAYNNNNRTG 1 cut(s) 797
MspI CCGG 3 cut(s) 422, 709, 840
MspR9I CCNGG 3 cut(s) 146, 709, 996
MvaI CCWGG 2 cut(s) 146, 996
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 6 cut(s) 65, 98, 464, 473, 698, 1022
NciI CCSGG 1 cut(s) 709
NdeII GATC 3 cut(s) 430, 513, 751
NlaIII CATG 7 cut(s) 113, 134, 238, 678, 775, 939, 970
NlaIV GGNNCC 3 cut(s) 52, 732, 910
NmuCI GTSAC 1 cut(s) 801
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 1 cut(s) 678
PciI ACATGT 1 cut(s) 674
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PfeI GAWTC 2 cut(s) 323, 973
Pfl23II CGTACG 1 cut(s) 354
PinAI ACCGGT 1 cut(s) 839
PkrI GCNGC 6 cut(s) 103, 315, 475, 569, 703, 706
Ppu21I YACGTR 2 cut(s) 354, 948
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 674
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 144, 994
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 2 cut(s) 144, 994
PspLI CGTACG 1 cut(s) 354
PspN4I GGNNCC 3 cut(s) 52, 732, 910
PspPI GGNCC 3 cut(s) 51, 865, 939
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 224, 256
PstI CTGCAG 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 567
RsaI GTAC 3 cut(s) 86, 356, 732
RsaNI GTAC 3 cut(s) 85, 355, 731
RseI CAYNNNNRTG 1 cut(s) 797
SaqAI TTAA 2 cut(s) 606, 923
SatI GCNGC 6 cut(s) 102, 314, 474, 568, 702, 705
Sau3AI GATC 3 cut(s) 430, 513, 751
Sau96I GGNCC 3 cut(s) 51, 865, 939
ScrFI CCNGG 3 cut(s) 146, 709, 996
SfaNI GCATC 4 cut(s) 68, 331, 353, 580
SfcI CTRYAG 1 cut(s) 465
SgrAI CRCCGGYG 1 cut(s) 839
SinI GGWCC 3 cut(s) 51, 865, 939
SmiMI CAYNNNNRTG 1 cut(s) 797
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
SnaBI TACGTA 1 cut(s) 354
SpeI ACTAGT 1 cut(s) 716
Sse9I AATT 6 cut(s) 215, 284, 509, 621, 637, 931
SsiI CCGC 4 cut(s) 76, 161, 336, 828
SspMI CTAG 1 cut(s) 717
StyD4I CCNGG 3 cut(s) 144, 707, 994
StyI CCWWGG 3 cut(s) 45, 54, 571
TaaI ACNGT 6 cut(s) 158, 224, 522, 545, 743, 880
TaiI ACGT 3 cut(s) 74, 356, 950
TaqI TCGA 2 cut(s) 210, 480
TasI AATT 6 cut(s) 215, 284, 509, 621, 637, 931
TfiI GAWTC 2 cut(s) 323, 973
Tru1I TTAA 2 cut(s) 606, 923
Tru9I TTAA 2 cut(s) 606, 923
TscAI CASTG 9 cut(s) 122, 163, 229, 325, 474, 525, 634, 670, 748
TseFI GTSAC 1 cut(s) 801
TseI GCWGC 6 cut(s) 101, 313, 473, 567, 701, 704
Tsp45I GTSAC 1 cut(s) 801
TspDTI ATGAA 4 cut(s) 137, 698, 924, 983
TspGWI ACGGA 3 cut(s) 389, 630, 775
TspRI CASTG 9 cut(s) 122, 163, 229, 325, 474, 525, 634, 670, 748
VpaK11BI GGWCC 3 cut(s) 51, 865, 939
XapI RAATTY 3 cut(s) 215, 284, 931
XceI RCATGY 1 cut(s) 678
XcmI CCANNNNNNNNNTGG 3 cut(s) 393, 500, 656
XspI CTAG 1 cut(s) 717
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.