MD05G1288400.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000341
Physical Location & Seq
Reverse (-)
42102054 .. 42107038
4985 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1288400.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGCGTTTGAAAGAAACATTTTAATTGTTGCCATACTCATCACCTTGGGGACCTTGGCATCTCAAGCCACGTCCCGCACATTGTACGAAGCTTCCATTGCTGCAAAACATGAGCAATGGATGGAAGAGTTTGGGCGCGTTTACCAGGACAGCGCAGAGAAGGAAAGGCGTTTCCACATATTCAAGAACAATGTGGAATTCGTGGAGAAATTCAACAGTGAAGGGAAGAAGACATACAAGCTAAGCATCAATCAATTTTCTGATATGACCCATGAAGAATTTCTCAGACATCATACTGGATACAAGAAGCCCACTGAATCAACCTCATCCGCAGATGCATCTTTTACGCACGAAAACCTAAGCCTGACGGATGATGTTCCCCCAAGCATTGACTGGAGGGAGCAAGATGCTGTTACTCCTGTAAAAGATCAAGGGGACTGTGGTTGTTGCTGGGCATTTTCTGCAGTGGCAGCAGTCGAAGGGATTACCAAAATCAAAACTGGCCAATTGATCTCACTATCTGAGCAACAACTTGTGGACTGTGATACAAGTAACTCTGGCTGCCAAGGTGGTGACATGGCTAAAGCCTTTGCATACATTCAACAGAACGGAGGAATTGCCAGTGAAGAAAATTACCCATACCAGGGTTCGGATGGAACATGTGATACGAACCTGGCAAATGAGGCTGCTGCCAAGATAACTAATTATGCAAGGGTAACTCCCAACAGTGAAACCGATTTGCTCAAGGCTGTATCCGTGCAACCAGTCTCCGTTGCCATCGCTGCCTCACAAGCCTTTCAGCTCTATTCCGGAGGCCTGTTCACTGATGATAGTTGTGGGACAGAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACGGAAGATGGCACCCCCTATTGGTTAATCAAGAACTCATGGGCCGAAAGGTGGGGTGATAATGGTTACATGAAGATTCTTAGAAACGCCGATGCCCCAGAAGGTGTTTGTGGCCTCGCTATGCAAGCTTCCTATCCAATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

343

Amino Acids

37.54

Weight (kDa)

4.77

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 4e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 126 - 341 2.8e-82 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 893
AccII CGCG 1 cut(s) 138
AccIII TCCGGA 1 cut(s) 809
AciI CCGC 2 cut(s) 76, 330
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 3 cut(s) 197, 209, 278
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 6 cut(s) 643, 644, 649, 870, 903, 933
AflIII ACRYGT 1 cut(s) 659
AgsI TTSAA 5 cut(s) 11, 184, 214, 602, 850
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 3 cut(s) 144, 642, 672
AluBI AGCT 4 cut(s) 92, 241, 802, 1010
AluI AGCT 4 cut(s) 92, 241, 802, 1010
Alw26I GTCTC 1 cut(s) 772
Aor13HI TCCGGA 1 cut(s) 809
AoxI GGCC 4 cut(s) 502, 814, 924, 994
ApeKI GCWGC 6 cut(s) 101, 470, 561, 686, 689, 782
ApoI RAATTY 3 cut(s) 197, 209, 278
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 2 cut(s) 138, 155
AspS9I GGNCC 2 cut(s) 51, 924
AsuHPI GGTGA 3 cut(s) 34, 584, 950
AvaII GGWCC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 893
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbsI GAAGAC 1 cut(s) 236
BbvI GCAGC 6 cut(s) 88, 482, 548, 673, 676, 769
BccI CCATC 5 cut(s) 115, 647, 785, 872, 884
BciT130I CCWGG 3 cut(s) 146, 644, 674
BciVI GTATCC 2 cut(s) 293, 763
BcoDI GTCTC 1 cut(s) 772
BfmI CTRYAG 1 cut(s) 462
BfuI GTATCC 2 cut(s) 293, 763
BisI GCNGC 6 cut(s) 102, 471, 562, 687, 690, 783
BlpI GCTNAGC 1 cut(s) 242
BlsI GCNGC 6 cut(s) 103, 472, 563, 688, 691, 784
Bme1390I CCNGG 3 cut(s) 146, 644, 674
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 51, 924
BmiI GGNNCC 2 cut(s) 52, 895
BmrFI CCNGG 3 cut(s) 146, 644, 674
BmsI GCATC 6 cut(s) 68, 255, 325, 347, 397, 964
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 48, 728
BsaJI CCNNGG 4 cut(s) 45, 54, 565, 643
BsaWI WCCGGW 1 cut(s) 809
Bsc4I CCNNNNNNNGG 6 cut(s) 643, 644, 649, 870, 903, 933
Bse1I ACTGG 5 cut(s) 301, 398, 505, 621, 764
Bse3DI GCAATG 2 cut(s) 96, 122
BseAI TCCGGA 1 cut(s) 809
BseBI CCWGG 3 cut(s) 146, 644, 674
BseDI CCNNGG 4 cut(s) 45, 54, 565, 643
BseGI GGATG 4 cut(s) 126, 326, 376, 658
BseLI CCNNNNNNNGG 6 cut(s) 643, 644, 649, 870, 903, 933
BseMI GCAATG 2 cut(s) 96, 122
BseMII CTCAG 2 cut(s) 298, 513
BseNI ACTGG 5 cut(s) 301, 398, 505, 621, 764
BseXI GCAGC 6 cut(s) 88, 482, 548, 673, 676, 769
BseYI CCCAGC 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 4 cut(s) 504, 816, 926, 996
BshNI GGYRCC 1 cut(s) 893
BsiSI CCGG 1 cut(s) 810
BslFI GGGAC 5 cut(s) 58, 64, 449, 853, 892
BslI CCNNNNNNNGG 6 cut(s) 643, 644, 649, 870, 903, 933
BsmAI GTCTC 1 cut(s) 772
BsmFI GGGAC 5 cut(s) 58, 64, 449, 853, 892
BsnI GGCC 4 cut(s) 504, 816, 926, 996
Bsp13I TCCGGA 1 cut(s) 809
Bsp143I GATC 2 cut(s) 427, 510
Bsp1720I GCTNAGC 1 cut(s) 242
BspACI CCGC 2 cut(s) 76, 330
BspANI GGCC 4 cut(s) 504, 816, 926, 996
BspCNI CTCAG 2 cut(s) 297, 514
BspEI TCCGGA 1 cut(s) 809
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 52, 895
BspMAI CTGCAG 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 893
BsrDI GCAATG 2 cut(s) 96, 122
BsrI ACTGG 5 cut(s) 301, 398, 505, 621, 764
BssECI CCNNGG 4 cut(s) 45, 54, 565, 643
BssMI GATC 2 cut(s) 427, 510
BssT1I CCWWGG 3 cut(s) 45, 54, 565
Bst2UI CCWGG 3 cut(s) 146, 644, 674
Bst4CI ACNGT 4 cut(s) 218, 440, 542, 728
Bst6I CTCTTC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 1008
BstDEI CTNAG 5 cut(s) 242, 284, 359, 522, 962
BstF5I GGATG 4 cut(s) 126, 326, 376, 658
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 2 cut(s) 138, 155
BstKTI GATC 2 cut(s) 430, 513
BstMAI GTCTC 1 cut(s) 772
BstMBI GATC 2 cut(s) 427, 510
BstMWI GCNNNNNNNGC 8 cut(s) 65, 98, 461, 470, 683, 782, 791, 1007
BstNI CCWGG 3 cut(s) 146, 644, 674
BstNSI RCATGY 1 cut(s) 663
BstSCI CCNGG 3 cut(s) 144, 642, 672
BstSFI CTRYAG 1 cut(s) 462
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 88, 482, 548, 673, 676, 769
BstV2I GAAGAC 1 cut(s) 236
BsuI GTATCC 2 cut(s) 293, 763
BsuRI GGCC 4 cut(s) 504, 816, 926, 996
BtgZI GCGATG 1 cut(s) 763
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 4 cut(s) 126, 326, 376, 658
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 6 cut(s) 223, 312, 471, 628, 733, 822
Cac8I GCNNGC 1 cut(s) 1008
CfoI GCGC 2 cut(s) 138, 155
Cfr13I GGNCC 2 cut(s) 51, 924
Csp6I GTAC 1 cut(s) 85
CviAII CATG 6 cut(s) 110, 272, 577, 660, 921, 952
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 5 cut(s) 242, 284, 359, 522, 962
DpnI GATC 2 cut(s) 429, 512
DpnII GATC 2 cut(s) 427, 510
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 45, 54, 565
Eco147I AGGCCT 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 144, 642, 672
EcoT14I CCWWGG 3 cut(s) 45, 54, 565
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 3 cut(s) 45, 54, 565
FaeI CATG 6 cut(s) 113, 275, 580, 663, 924, 955
FaqI GGGAC 5 cut(s) 58, 64, 449, 853, 892
FatI CATG 6 cut(s) 109, 271, 576, 659, 920, 951
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 6 cut(s) 102, 471, 562, 687, 690, 783
FokI GGATG 4 cut(s) 133, 313, 383, 665
Fsp4HI GCNGC 6 cut(s) 102, 471, 562, 687, 690, 783
GlaI GCGC 2 cut(s) 137, 154
GluI GCNGC 6 cut(s) 102, 471, 562, 687, 690, 783
GsaI CCCAGC 1 cut(s) 454
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 4 cut(s) 504, 816, 926, 996
HapII CCGG 1 cut(s) 810
HhaI GCGC 2 cut(s) 138, 155
Hin1II CATG 6 cut(s) 113, 275, 580, 663, 924, 955
Hin6I GCGC 2 cut(s) 136, 153
HinP1I GCGC 2 cut(s) 136, 153
HindIII AAGCTT 2 cut(s) 90, 1008
HinfI GANTC 2 cut(s) 317, 958
HpaII CCGG 1 cut(s) 810
HphI GGTGA 3 cut(s) 34, 584, 950
Hpy166II GTNNAC 3 cut(s) 142, 538, 822
Hpy188I TCNGA 4 cut(s) 262, 287, 523, 652
Hpy188III TCNNGA 3 cut(s) 184, 810, 913
Hpy8I GTNNAC 3 cut(s) 142, 538, 822
HpyAV CCTTC 4 cut(s) 154, 215, 473, 977
HpyCH4III ACNGT 4 cut(s) 218, 440, 542, 728
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 7 cut(s) 104, 338, 464, 593, 710, 760, 1006
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 98, 461, 470, 683, 782, 791, 1007
HpyF3I CTNAG 5 cut(s) 242, 284, 359, 522, 962
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 6 cut(s) 113, 275, 580, 663, 924, 955
HspAI GCGC 2 cut(s) 136, 153
Kpn2I TCCGGA 1 cut(s) 809
Kzo9I GATC 2 cut(s) 427, 510
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 6 cut(s) 88, 482, 548, 673, 676, 769
LweI GCATC 6 cut(s) 68, 255, 325, 347, 397, 964
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 6 cut(s) 412, 551, 572, 715, 859, 947
MalI GATC 2 cut(s) 429, 512
MboI GATC 2 cut(s) 427, 510
MboII GAAGA 7 cut(s) 137, 238, 241, 287, 638, 899, 967
MfeI CAATTG 1 cut(s) 506
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
MnlI CCTC 7 cut(s) 334, 390, 605, 676, 796, 806, 1007
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 809
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 504
MseI TTAA 2 cut(s) 23, 908
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 1 cut(s) 810
MspR9I CCNGG 3 cut(s) 146, 644, 674
MunI CAATTG 1 cut(s) 506
MvaI CCWGG 3 cut(s) 146, 644, 674
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 8 cut(s) 65, 98, 461, 470, 683, 782, 791, 1007
NdeII GATC 2 cut(s) 427, 510
NlaIII CATG 6 cut(s) 113, 275, 580, 663, 924, 955
NlaIV GGNNCC 2 cut(s) 52, 895
NmuCI GTSAC 1 cut(s) 572
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 663
PceI AGGCCT 1 cut(s) 816
PciI ACATGT 1 cut(s) 659
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 317, 958
PkrI GCNGC 6 cut(s) 103, 472, 563, 688, 691, 784
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 659
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 144, 642, 672
PspFI CCCAGC 1 cut(s) 450
PspGI CCWGG 3 cut(s) 144, 642, 672
PspN4I GGNNCC 2 cut(s) 52, 895
PspPI GGNCC 2 cut(s) 51, 924
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 649, 681
PstI CTGCAG 1 cut(s) 466
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SaqAI TTAA 2 cut(s) 23, 908
SatI GCNGC 6 cut(s) 102, 471, 562, 687, 690, 783
Sau3AI GATC 2 cut(s) 427, 510
Sau96I GGNCC 2 cut(s) 51, 924
ScrFI CCNGG 3 cut(s) 146, 644, 674
SfaNI GCATC 6 cut(s) 68, 255, 325, 347, 397, 964
SfcI CTRYAG 1 cut(s) 462
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 63, 743
SmoI CTYRAG 2 cut(s) 63, 743
SseBI AGGCCT 1 cut(s) 816
SsiI CCGC 2 cut(s) 76, 330
StuI AGGCCT 1 cut(s) 816
StyD4I CCNGG 3 cut(s) 144, 642, 672
StyI CCWWGG 3 cut(s) 45, 54, 565
TaaI ACNGT 4 cut(s) 218, 440, 542, 728
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 477
TfiI GAWTC 2 cut(s) 317, 958
Tru1I TTAA 2 cut(s) 23, 908
Tru9I TTAA 2 cut(s) 23, 908
TscAI CASTG 6 cut(s) 223, 319, 471, 628, 733, 829
TseFI GTSAC 1 cut(s) 572
TseI GCWGC 6 cut(s) 101, 470, 561, 686, 689, 782
Tsp45I GTSAC 1 cut(s) 572
TspDTI ATGAA 2 cut(s) 288, 968
TspGWI ACGGA 5 cut(s) 383, 624, 745, 760, 899
TspRI CASTG 6 cut(s) 223, 319, 471, 628, 733, 829
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 3 cut(s) 197, 209, 278
XceI RCATGY 1 cut(s) 663
XcmI CCANNNNNNNNNTGG 2 cut(s) 390, 650
XmnI GAANNNNTTC 1 cut(s) 279
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.