MD05G1287600.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000341
Physical Location & Seq
Reverse (-)
42009191 .. 42009998
808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1287600.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGCGTTTGAAAGAAACATTTTAATTGTTGCCATACTCATCACCTTGGGGACCTTGGCATCTCAAGCCACGTCCCGCACATTGTACGAAGCTTCCATTGCTGCAAAACATGAGCAATGGATGGAAGAGTTTGGGCGCGTTTACCAGGACAGTGCAGAGACGGAAAGGCGTTTCCACATATTCAAGAACAATGTGGAATTCGTGGAGAAATTCAACAGTGAAGGGAAGAAGACATACAAGCTAAGCATCAATCAATTTTCTGATATGACCCATGAAGAATTTCTCAGACATCATACTGGATACAAGAAGCCCACTGAATCAACCTCATCCGCAGATGCATCTTTTACGCACGAAAACCTAAGCCTGACGGATGTTCCCCCAAGCATTGACTGGAGGGAGCAAGATGCTGTTACTCCTGTAAAAGATCAAGGGGACTGTGGTTGTTGCTGGGCATTTTCTGCAGTGGCAGCAGTCGAAGGGATTACCAAAATCAAAACTGGCCAATTGATCTCACTGTCTGAGCAACAACTTGTGGACTGTGATACAAGTAACTCTGGCTGCCAAGGTGGTGACATGGCTAAAGCCTTTGCATACATTCAACAGAACGGAGGAATTGCCAGTGAAGAAAATTACCCATACCAGGGTTCGGATGGAACATGTGATACGAACCTGGCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

227

Amino Acids

25.08

Weight (kDa)

4.91

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 3.7e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 223 2.2e-41 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 76, 330
AcoI YGGCCR 1 cut(s) 499
AcsI RAATTY 3 cut(s) 197, 209, 278
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 3 cut(s) 640, 641, 646
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 4 cut(s) 11, 184, 214, 599
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 3 cut(s) 144, 639, 669
AluBI AGCT 2 cut(s) 92, 241
AluI AGCT 2 cut(s) 92, 241
Alw26I GTCTC 1 cut(s) 152
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 3 cut(s) 101, 467, 558
ApoI RAATTY 3 cut(s) 197, 209, 278
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 1 cut(s) 51
AsuHPI GGTGA 2 cut(s) 34, 581
AvaII GGWCC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 501
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbsI GAAGAC 1 cut(s) 236
BbvI GCAGC 3 cut(s) 88, 479, 545
BccI CCATC 2 cut(s) 115, 644
BciT130I CCWGG 3 cut(s) 146, 641, 671
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 459
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 3 cut(s) 102, 468, 559
BlpI GCTNAGC 1 cut(s) 242
BlsI GCNGC 3 cut(s) 103, 469, 560
Bme1390I CCNGG 3 cut(s) 146, 641, 671
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 52
BmrFI CCNGG 3 cut(s) 146, 641, 671
BmsI GCATC 5 cut(s) 68, 255, 325, 347, 394
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 412
Bpu10I CCTNAGC 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 48
BsaJI CCNNGG 4 cut(s) 45, 54, 562, 640
Bsc4I CCNNNNNNNGG 3 cut(s) 640, 641, 646
Bse1I ACTGG 4 cut(s) 301, 395, 502, 618
Bse3DI GCAATG 2 cut(s) 96, 122
BseBI CCWGG 3 cut(s) 146, 641, 671
BseDI CCNNGG 4 cut(s) 45, 54, 562, 640
BseGI GGATG 4 cut(s) 126, 326, 376, 655
BseLI CCNNNNNNNGG 3 cut(s) 640, 641, 646
BseMI GCAATG 2 cut(s) 96, 122
BseMII CTCAG 2 cut(s) 298, 510
BseNI ACTGG 4 cut(s) 301, 395, 502, 618
BseXI GCAGC 3 cut(s) 88, 479, 545
BseYI CCCAGC 1 cut(s) 447
BsgI GTGCAG 1 cut(s) 174
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 501
BslFI GGGAC 3 cut(s) 58, 64, 446
BslI CCNNNNNNNGG 3 cut(s) 640, 641, 646
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 3 cut(s) 58, 64, 446
BsnI GGCC 1 cut(s) 501
Bsp143I GATC 2 cut(s) 424, 507
Bsp1720I GCTNAGC 1 cut(s) 242
BspACI CCGC 2 cut(s) 76, 330
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 2 cut(s) 297, 511
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 52
BspMAI CTGCAG 1 cut(s) 463
BsrDI GCAATG 2 cut(s) 96, 122
BsrI ACTGG 4 cut(s) 301, 395, 502, 618
BssECI CCNNGG 4 cut(s) 45, 54, 562, 640
BssMI GATC 2 cut(s) 424, 507
BssT1I CCWWGG 3 cut(s) 45, 54, 562
Bst2UI CCWGG 3 cut(s) 146, 641, 671
Bst4CI ACNGT 5 cut(s) 152, 218, 437, 516, 539
Bst6I CTCTTC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 458
BstDEI CTNAG 4 cut(s) 242, 284, 359, 519
BstF5I GGATG 4 cut(s) 126, 326, 376, 655
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 2 cut(s) 427, 510
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 2 cut(s) 424, 507
BstMWI GCNNNNNNNGC 4 cut(s) 65, 98, 458, 467
BstNI CCWGG 3 cut(s) 146, 641, 671
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 3 cut(s) 144, 639, 669
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 88, 479, 545
BstV2I GAAGAC 1 cut(s) 236
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 501
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 4 cut(s) 126, 326, 376, 655
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 6 cut(s) 157, 223, 312, 468, 512, 625
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 85
CviAII CATG 4 cut(s) 110, 272, 574, 657
CviJI RGCY 9 cut(s) 68, 92, 241, 310, 363, 501, 558, 578, 584
CviKI_1 RGCY 9 cut(s) 68, 92, 241, 310, 363, 501, 558, 578, 584
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 4 cut(s) 242, 284, 359, 519
DpnI GATC 2 cut(s) 426, 509
DpnII GATC 2 cut(s) 424, 507
EaeI YGGCCR 1 cut(s) 499
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 45, 54, 562
Eco47I GGWCC 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 144, 639, 669
EcoT14I CCWWGG 3 cut(s) 45, 54, 562
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 3 cut(s) 45, 54, 562
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 4 cut(s) 113, 275, 577, 660
FaqI GGGAC 3 cut(s) 58, 64, 446
FatI CATG 4 cut(s) 109, 271, 573, 656
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 3 cut(s) 102, 468, 559
FokI GGATG 4 cut(s) 133, 313, 383, 662
Fsp4HI GCNGC 3 cut(s) 102, 468, 559
GlaI GCGC 1 cut(s) 137
GluI GCNGC 3 cut(s) 102, 468, 559
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 1 cut(s) 412
HaeIII GGCC 1 cut(s) 501
HhaI GCGC 1 cut(s) 138
Hin1II CATG 4 cut(s) 113, 275, 577, 660
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 1 cut(s) 317
HphI GGTGA 2 cut(s) 34, 581
Hpy166II GTNNAC 2 cut(s) 142, 535
Hpy188I TCNGA 4 cut(s) 262, 287, 520, 649
Hpy188III TCNNGA 1 cut(s) 184
Hpy8I GTNNAC 2 cut(s) 142, 535
HpyAV CCTTC 2 cut(s) 215, 470
HpyCH4III ACNGT 5 cut(s) 152, 218, 437, 516, 539
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 5 cut(s) 104, 155, 338, 461, 590
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 98, 458, 467
HpyF3I CTNAG 4 cut(s) 242, 284, 359, 519
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 4 cut(s) 113, 275, 577, 660
HspAI GCGC 1 cut(s) 136
Kzo9I GATC 2 cut(s) 424, 507
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 3 cut(s) 88, 479, 545
LweI GCATC 5 cut(s) 68, 255, 325, 347, 394
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 3 cut(s) 409, 548, 569
MalI GATC 2 cut(s) 426, 509
MboI GATC 2 cut(s) 424, 507
MboII GAAGA 5 cut(s) 137, 238, 241, 287, 635
MfeI CAATTG 1 cut(s) 503
MlsI TGGCCA 1 cut(s) 501
MluCI AATT 8 cut(s) 24, 197, 209, 254, 278, 503, 612, 628
MluNI TGGCCA 1 cut(s) 501
MnlI CCTC 3 cut(s) 334, 387, 602
Mox20I TGGCCA 1 cut(s) 501
Mph1103I ATGCAT 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 501
MseI TTAA 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 501
MspR9I CCNGG 3 cut(s) 146, 641, 671
MunI CAATTG 1 cut(s) 503
MvaI CCWGG 3 cut(s) 146, 641, 671
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 4 cut(s) 65, 98, 458, 467
NdeII GATC 2 cut(s) 424, 507
NlaIII CATG 4 cut(s) 113, 275, 577, 660
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 1 cut(s) 569
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 660
PciI ACATGT 1 cut(s) 656
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 3 cut(s) 103, 469, 560
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 656
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 144, 639, 669
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 3 cut(s) 144, 639, 669
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 51
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 646, 678
PstI CTGCAG 1 cut(s) 463
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 3 cut(s) 102, 468, 559
Sau3AI GATC 2 cut(s) 424, 507
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 3 cut(s) 146, 641, 671
SetI ASST 9 cut(s) 47, 56, 74, 94, 243, 326, 360, 568, 672
SfaNI GCATC 5 cut(s) 68, 255, 325, 347, 394
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse9I AATT 8 cut(s) 24, 197, 209, 254, 278, 503, 612, 628
SsiI CCGC 2 cut(s) 76, 330
StyD4I CCNGG 3 cut(s) 144, 639, 669
StyI CCWWGG 3 cut(s) 45, 54, 562
TaaI ACNGT 5 cut(s) 152, 218, 437, 516, 539
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 474
TasI AATT 8 cut(s) 24, 197, 209, 254, 278, 503, 612, 628
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 6 cut(s) 157, 223, 319, 468, 519, 625
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 3 cut(s) 101, 467, 558
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 1 cut(s) 288
TspGWI ACGGA 3 cut(s) 176, 383, 621
TspRI CASTG 6 cut(s) 157, 223, 319, 468, 519, 625
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 3 cut(s) 197, 209, 278
XceI RCATGY 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 647
XmnI GAANNNNTTC 1 cut(s) 279
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.