MD10G1282100.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000341
Physical Location & Seq
Forward (+)
37278071 .. 37279298
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1282100.v1.1.491

Sequence Viewer

Length: 1095 bp
ATGGCTTTTGAAAGAAAACTTTTGATTGTTACCATACTCATCACCTTGGGGACTTTGGCATCTGAAGCCACGTCCTACACATTGTACCAAGCTTCCATTGCTGCAAAACATGAGCAGTGGATAAAAAAGCATGGACGAGTTTACCAGGACAGCGTGGAGAAGGAAAGGCGTTTCCACATATTCAAAAACAATGTGGAGTTTGTCGAGAAATTCAACAGTGAAGGGAACATGACTTACAGCCTTTGCGTCAACCGATTTTCGGATATGAGCTATGACGAATTCCTCAGGTATTATACTGGATACAAGCAGCCCACTGAATCAACCTCATCCGCAGATGCATCATTTACGTACGAAAACCTAAGCCCGACGGATGTTCCCCCAAGCATTGACTGGAGGGACAATGGAGCTGTTACTCCAGTAAAAGATCAAGGGCGCTGTGGTTGTGTCTGGGCATTTTCCGCAGTGGCAGCAGTCGAAGGGATCATCCAAATCAAAACCGGCAAATTGATATCACTGTCTGAGCAACAACTTGTGAGCTGTGATACAATTAACAATGGCTGCCAAGGTGGTTCGATGGATAAAGCCTATGCATACATTCAACAAAACGGAGGAATTACCAGTGAAGAAAATTACCCATACCAGAGTTTAAATGGCAGTGCCGGAACATGTGATATGAACAAGGCATATAAGGCTGCTGCCCGGATAACTGGTTATGCAGAGGTACTTTCCATCAATGAAACCGAGCTACTCAAGGCTGTGTCCATGCAACCAGTCTCTGTTGGCATCCATGCCTCACCAGCCTTTCAGCAATATTCAGGCGGGGTGTTCACCGGTGATGATTGTGGGACAGAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCACCCCCTATTGGTTAATCAAGAATTCATGGTCCGATACGTGGGGTGAGAATGGCTACATGAAGATTCTTAGAAACGCTGATGCCCCAGGTGGTGTTTGTGGCCTCGCTACGAGAGCTTCCTATCCGACTACAAGTACTACAGACCAACCCATAGGCTGTGAAAATGCCTGTATTTTTTACTTGATAAAAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

365

Amino Acids

39.98

Weight (kDa)

5.09

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 1.1e-17 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 343 2.4e-78 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 899
AciI CCGC 3 cut(s) 330, 459, 819
AclWI GGATC 1 cut(s) 488
AcsI RAATTY 3 cut(s) 209, 278, 922
AcuI CTGAAG 2 cut(s) 84, 912
AfaI GTAC 4 cut(s) 86, 350, 723, 1036
AfiI CCNNNNNNNGG 4 cut(s) 259, 876, 909, 939
AflIII ACRYGT 1 cut(s) 665
AgeI ACCGGT 1 cut(s) 830
AgsI TTSAA 5 cut(s) 11, 184, 214, 599, 856
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 2 cut(s) 144, 985
AluBI AGCT 6 cut(s) 92, 270, 407, 537, 745, 1016
AluI AGCT 6 cut(s) 92, 270, 407, 537, 745, 1016
Alw26I GTCTC 1 cut(s) 778
AlwI GGATC 1 cut(s) 488
AlwNI CAGNNNCTG 1 cut(s) 776
AoxI GGCC 1 cut(s) 1000
ApeKI GCWGC 6 cut(s) 101, 307, 467, 558, 692, 695
ApoI RAATTY 3 cut(s) 209, 278, 922
AsiGI ACCGGT 1 cut(s) 830
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 1 cut(s) 930
AsuC2I CCSGG 1 cut(s) 700
AsuHPI GGTGA 5 cut(s) 34, 786, 820, 845, 956
AvaII GGWCC 1 cut(s) 930
AxyI CCTNAGG 1 cut(s) 284
BanI GGYRCC 1 cut(s) 899
BbvI GCAGC 6 cut(s) 88, 319, 479, 545, 679, 682
BccI CCATC 4 cut(s) 568, 737, 878, 890
BciT130I CCWGG 2 cut(s) 146, 987
BciVI GTATCC 1 cut(s) 293
BcnI CCSGG 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 778
BfmI CTRYAG 1 cut(s) 1038
BfoI RGCGCY 1 cut(s) 436
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 6 cut(s) 102, 308, 468, 559, 693, 696
BlsI GCNGC 6 cut(s) 103, 309, 469, 560, 694, 697
BmcAI AGTACT 1 cut(s) 1036
Bme1390I CCNGG 3 cut(s) 146, 700, 987
Bme18I GGWCC 1 cut(s) 930
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 930
BmiI GGNNCC 1 cut(s) 901
BmrFI CCNGG 3 cut(s) 146, 700, 987
BmsI GCATC 5 cut(s) 68, 325, 347, 792, 970
BpmI CTGGAG 2 cut(s) 399, 412
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 700
BsaAI YACGTR 2 cut(s) 348, 939
BsaJI CCNNGG 3 cut(s) 45, 562, 985
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 876, 909, 939
Bse118I RCCGGY 2 cut(s) 497, 830
Bse1I ACTGG 6 cut(s) 301, 395, 416, 618, 712, 770
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 2 cut(s) 146, 987
BseDI CCNNGG 3 cut(s) 45, 562, 985
BseGI GGATG 4 cut(s) 326, 376, 483, 783
BseLI CCNNNNNNNGG 4 cut(s) 259, 876, 909, 939
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 298, 510
BseNI ACTGG 6 cut(s) 301, 395, 416, 618, 712, 770
BseXI GCAGC 6 cut(s) 88, 319, 479, 545, 679, 682
BshFI GGCC 1 cut(s) 1002
BshNI GGYRCC 1 cut(s) 899
BshTI ACCGGT 1 cut(s) 830
BsiSI CCGG 4 cut(s) 498, 660, 700, 831
BsiWI CGTACG 1 cut(s) 348
BslFI GGGAC 4 cut(s) 64, 410, 859, 898
BslI CCNNNNNNNGG 4 cut(s) 259, 876, 909, 939
BsmAI GTCTC 1 cut(s) 778
BsmFI GGGAC 4 cut(s) 64, 410, 859, 898
BsnI GGCC 1 cut(s) 1002
Bsp143I GATC 2 cut(s) 424, 480
BspACI CCGC 3 cut(s) 330, 459, 819
BspANI GGCC 1 cut(s) 1002
BspCNI CTCAG 2 cut(s) 297, 511
BspLI GGNNCC 1 cut(s) 901
BspPI GGATC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 899
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 2 cut(s) 497, 830
BsrI ACTGG 6 cut(s) 301, 395, 416, 618, 712, 770
BssAI RCCGGY 2 cut(s) 497, 830
BssECI CCNNGG 3 cut(s) 45, 562, 985
BssMI GATC 2 cut(s) 424, 480
BssT1I CCWWGG 2 cut(s) 45, 562
Bst2UI CCWGG 2 cut(s) 146, 987
Bst4CI ACNGT 2 cut(s) 218, 516
BstBAI YACGTR 2 cut(s) 348, 939
BstDEI CTNAG 4 cut(s) 284, 359, 519, 968
BstF5I GGATG 4 cut(s) 326, 376, 483, 783
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 2 cut(s) 427, 483
BstMAI GTCTC 1 cut(s) 778
BstMBI GATC 2 cut(s) 424, 480
BstMWI GCNNNNNNNGC 7 cut(s) 65, 98, 458, 467, 689, 797, 1013
BstNI CCWGG 2 cut(s) 146, 987
BstNSI RCATGY 1 cut(s) 669
BstSCI CCNGG 3 cut(s) 144, 698, 985
BstSFI CTRYAG 1 cut(s) 1038
BstSNI TACGTA 1 cut(s) 348
BstV1I GCAGC 6 cut(s) 88, 319, 479, 545, 679, 682
Bsu36I CCTNAGG 1 cut(s) 284
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 1002
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 4 cut(s) 326, 376, 483, 783
BtsI GCAGTG 3 cut(s) 122, 468, 661
BtsIMutI CAGTG 7 cut(s) 122, 223, 312, 468, 512, 625, 661
CaiI CAGNNNCTG 1 cut(s) 776
CfoI GCGC 1 cut(s) 435
Cfr10I RCCGGY 2 cut(s) 497, 830
Cfr13I GGNCC 1 cut(s) 930
CseI GACGC 1 cut(s) 235
Csp6I GTAC 4 cut(s) 85, 349, 722, 1035
CspAI ACCGGT 1 cut(s) 830
CviAII CATG 8 cut(s) 110, 131, 229, 666, 763, 788, 927, 958
CviQI GTAC 4 cut(s) 85, 349, 722, 1035
DdeI CTNAG 4 cut(s) 284, 359, 519, 968
DpnI GATC 2 cut(s) 426, 482
DpnII GATC 2 cut(s) 424, 480
DraI TTTAAA 1 cut(s) 648
Eco105I TACGTA 1 cut(s) 348
Eco130I CCWWGG 2 cut(s) 45, 562
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 930
Eco57I CTGAAG 2 cut(s) 84, 912
Eco81I CCTNAGG 1 cut(s) 284
EcoRI GAATTC 2 cut(s) 278, 922
EcoRII CCWGG 2 cut(s) 144, 985
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 2 cut(s) 45, 562
EcoT22I ATGCAT 2 cut(s) 340, 592
ErhI CCWWGG 2 cut(s) 45, 562
FaeI CATG 8 cut(s) 113, 134, 232, 669, 766, 791, 930, 961
FaqI GGGAC 4 cut(s) 64, 410, 859, 898
FatI CATG 8 cut(s) 109, 130, 228, 665, 762, 787, 926, 957
FauI CCCGC 1 cut(s) 812
Fnu4HI GCNGC 6 cut(s) 102, 308, 468, 559, 693, 696
FokI GGATG 4 cut(s) 313, 383, 470, 770
Fsp4HI GCNGC 6 cut(s) 102, 308, 468, 559, 693, 696
GlaI GCGC 1 cut(s) 434
GluI GCNGC 6 cut(s) 102, 308, 468, 559, 693, 696
GsuI CTGGAG 2 cut(s) 399, 412
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 1 cut(s) 1002
HapII CCGG 4 cut(s) 498, 660, 700, 831
HgaI GACGC 1 cut(s) 235
HhaI GCGC 1 cut(s) 435
Hin1II CATG 8 cut(s) 113, 134, 232, 669, 766, 791, 930, 961
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 2 cut(s) 317, 964
HpaII CCGG 4 cut(s) 498, 660, 700, 831
HphI GGTGA 5 cut(s) 34, 786, 820, 845, 956
Hpy166II GTNNAC 3 cut(s) 142, 250, 828
Hpy188I TCNGA 5 cut(s) 64, 262, 520, 934, 1026
Hpy188III TCNNGA 2 cut(s) 205, 919
Hpy8I GTNNAC 3 cut(s) 142, 250, 828
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 3 cut(s) 154, 215, 470
HpyCH4III ACNGT 2 cut(s) 218, 516
HpyCH4IV ACGT 3 cut(s) 71, 347, 938
HpyCH4V TGCA 5 cut(s) 104, 338, 590, 716, 766
HpyF10VI GCNNNNNNNGC 7 cut(s) 65, 98, 458, 467, 689, 797, 1013
HpyF3I CTNAG 4 cut(s) 284, 359, 519, 968
HpySE526I ACGT 3 cut(s) 71, 347, 938
Hsp92II CATG 8 cut(s) 113, 134, 232, 669, 766, 791, 930, 961
HspAI GCGC 1 cut(s) 433
Kzo9I GATC 2 cut(s) 424, 480
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 6 cut(s) 88, 319, 479, 545, 679, 682
LweI GCATC 5 cut(s) 68, 325, 347, 792, 970
MaeII ACGT 3 cut(s) 71, 347, 938
MaeIII GTNAC 3 cut(s) 28, 409, 865
MalI GATC 2 cut(s) 426, 482
MboI GATC 2 cut(s) 424, 480
MboII GAAGA 3 cut(s) 635, 905, 973
MluCI AATT 8 cut(s) 209, 278, 503, 546, 612, 628, 851, 922
MmeI TCCRAC 1 cut(s) 1049
MnlI CCTC 7 cut(s) 293, 334, 387, 602, 712, 802, 1013
Mph1103I ATGCAT 2 cut(s) 340, 592
MseI TTAA 3 cut(s) 549, 647, 914
MspI CCGG 4 cut(s) 498, 660, 700, 831
MspR9I CCNGG 3 cut(s) 146, 700, 987
MvaI CCWGG 2 cut(s) 146, 987
MwoI GCNNNNNNNGC 7 cut(s) 65, 98, 458, 467, 689, 797, 1013
NciI CCSGG 1 cut(s) 700
NdeII GATC 2 cut(s) 424, 480
NlaIII CATG 8 cut(s) 113, 134, 232, 669, 766, 791, 930, 961
NlaIV GGNNCC 1 cut(s) 901
NsiI ATGCAT 2 cut(s) 340, 592
NspI RCATGY 1 cut(s) 669
PciI ACATGT 1 cut(s) 665
PfeI GAWTC 2 cut(s) 317, 964
Pfl23II CGTACG 1 cut(s) 348
PinAI ACCGGT 1 cut(s) 830
PkrI GCNGC 6 cut(s) 103, 309, 469, 560, 694, 697
Ppu21I YACGTR 2 cut(s) 348, 939
PscI ACATGT 1 cut(s) 665
Psp6I CCWGG 2 cut(s) 144, 985
PspGI CCWGG 2 cut(s) 144, 985
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 901
PspPI GGNCC 1 cut(s) 930
PsrI GAACNNNNNNTAC 2 cut(s) 218, 250
PstNI CAGNNNCTG 1 cut(s) 776
RsaI GTAC 4 cut(s) 86, 350, 723, 1036
RsaNI GTAC 4 cut(s) 85, 349, 722, 1035
SaqAI TTAA 3 cut(s) 549, 647, 914
SatI GCNGC 6 cut(s) 102, 308, 468, 559, 693, 696
Sau3AI GATC 2 cut(s) 424, 480
Sau96I GGNCC 1 cut(s) 930
ScaI AGTACT 1 cut(s) 1036
ScrFI CCNGG 3 cut(s) 146, 700, 987
SfaNI GCATC 5 cut(s) 68, 325, 347, 792, 970
SfcI CTRYAG 1 cut(s) 1038
SgrAI CRCCGGYG 1 cut(s) 830
SinI GGWCC 1 cut(s) 930
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
SnaBI TACGTA 1 cut(s) 348
Sse9I AATT 8 cut(s) 209, 278, 503, 546, 612, 628, 851, 922
SsiI CCGC 3 cut(s) 330, 459, 819
SspI AATATT 1 cut(s) 812
StyD4I CCNGG 3 cut(s) 144, 698, 985
StyI CCWWGG 2 cut(s) 45, 562
TaaI ACNGT 2 cut(s) 218, 516
TaiI ACGT 3 cut(s) 74, 350, 941
TaqI TCGA 3 cut(s) 204, 474, 572
TasI AATT 8 cut(s) 209, 278, 503, 546, 612, 628, 851, 922
TatI WGTACW 1 cut(s) 1034
TfiI GAWTC 2 cut(s) 317, 964
Tru1I TTAA 3 cut(s) 549, 647, 914
Tru9I TTAA 3 cut(s) 549, 647, 914
TscAI CASTG 7 cut(s) 122, 223, 319, 468, 519, 625, 661
TseI GCWGC 6 cut(s) 101, 307, 467, 558, 692, 695
TspDTI ATGAA 4 cut(s) 689, 750, 915, 974
TspGWI ACGGA 2 cut(s) 383, 621
TspRI CASTG 7 cut(s) 122, 223, 319, 468, 519, 625, 661
VpaK11BI GGWCC 1 cut(s) 930
XapI RAATTY 3 cut(s) 209, 278, 922
XceI RCATGY 1 cut(s) 669
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 647
ZrmI AGTACT 1 cut(s) 1036
Zsp2I ATGCAT 2 cut(s) 340, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.