Rh5BG165800

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000341
Physical Location & Seq
Forward (+)
17419089 .. 17420819
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG165800.1

Sequence Viewer

Length: 1044 bp
ATGGCTTTTGAAAAAATCCTTGTGATGATTGCCATATTCATCATCTTGGGGACCTTGGCATCCCAAGCCACATCTCGCGCATTGTACAAAGCTTCCATTGCTGAGAAACATGAGCAATGGATGGCCGAGTATGGGCGTGTCTACCTGGACAGCACAGAGAAGGAAAGACGTTTTGCCATATTCAAGAAGAATGTAGAGTTTGTGGAGAAATTCAACATTGACAGGGAAAAGACGTACAAGTTGAGCGTCAATCAGTTCTCTGATATGACCAATGAAGAATTCCTCAGACATCATACTGGATACAAAACGCCTGCCGGCTCCAGTTTAACATCCTCATCCGAAGATAAGTCTTTTATATATCGAAGCCTGTCCATTGATGATATTCCAGCTAGCCGTGACTGGAGGGAACAAGGAGCCGTCACCTCTATTAAGGATCAAGGTTCATGTAATGTTTGCTGGGCATTTGCTGCGGCAGCAGCCGTGGAAGGGATTATGAAAATCAAAATCGGCCAATTGGTCTCGCTGTCCGAGCAACAACTTTTGGAGTGTGACCGCAATATCGGCTGCGATAGCGGTGACATCCGATCAGCTTTTGAATACATAAAGACAAATGGAGGAATTGCTAGTGAAGAAAAGTACCCATACCTGAGCACTTCTGACATGTTAACGGTCAAAGAATGCGACACTCGAAAGGAAACTATGCAAGCTGCCCGGATAACTGGTTATGAAAATGTAACATCCAACAATCAAAATGCTCTACTCAAGGCTGTTTCCATGCAACCAGTCTCGACTGCCATCGACGGTCATGGAATGGAATTTCAGTCATACGGAGGTGGAGTGTTTAAAGGTCCTTGCGGTCAGAAACTGAACCATGCTGTCACAATTATTGGGTACGGGACTGAAAACGGCACCAACTACTGGCTAATTAAGAATTCATGGGGGGACAAGTGGGGTGAAAAGGGCTACATGAAAATTCTTAGAACTGAAACATATGGTGCAGGTCTTTGTGGCCTTGCGATGTATGCTTCCTATCCGATTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

347

Amino Acids

38.68

Weight (kDa)

6.27

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 2.8e-19 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 128 - 346 1.8e-73 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 989
AccB1I GGYRCC 1 cut(s) 908
AccB7I CCANNNNNTGG 1 cut(s) 918
AccI GTMKAC 1 cut(s) 141
AccII CGCG 1 cut(s) 78
AciI CCGC 4 cut(s) 470, 553, 573, 855
AclWI GGATC 1 cut(s) 441
AcoI YGGCCR 2 cut(s) 123, 508
AcsI RAATTY 5 cut(s) 209, 278, 815, 931, 972
AfaI GTAC 4 cut(s) 86, 236, 638, 893
AfiI CCNNNNNNNGG 3 cut(s) 132, 486, 918
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 4 cut(s) 11, 184, 214, 596
AjnI CCWGG 1 cut(s) 144
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 4 cut(s) 92, 389, 590, 707
AluI AGCT 4 cut(s) 92, 389, 590, 707
Alw21I GWGCWC 1 cut(s) 653
Alw26I GTCTC 2 cut(s) 523, 790
AlwI GGATC 1 cut(s) 441
AlwNI CAGNNNCTG 1 cut(s) 865
AoxI GGCC 3 cut(s) 123, 508, 1009
ApeKI GCWGC 5 cut(s) 467, 473, 476, 564, 707
ApoI RAATTY 5 cut(s) 209, 278, 815, 931, 972
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 51, 848
AsuC2I CCSGG 1 cut(s) 712
AsuHPI GGTGA 3 cut(s) 412, 587, 965
AsuNHI GCTAGC 1 cut(s) 389
AvaII GGWCC 2 cut(s) 51, 848
BanI GGYRCC 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 5 cut(s) 454, 485, 488, 551, 694
BccI CCATC 2 cut(s) 115, 803
BceAI ACGGC 4 cut(s) 378, 401, 464, 922
BciT130I CCWGG 1 cut(s) 146
BciVI GTATCC 1 cut(s) 293
BcnI CCSGG 1 cut(s) 712
BcoDI GTCTC 2 cut(s) 523, 790
BfaI CTAG 2 cut(s) 390, 624
BfuAI ACCTGC 1 cut(s) 989
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 6 cut(s) 468, 471, 474, 477, 565, 708
BlsI GCNGC 6 cut(s) 469, 472, 475, 478, 566, 709
Bme1390I CCNGG 2 cut(s) 146, 712
Bme18I GGWCC 2 cut(s) 51, 848
BmgT120I GGNCC 2 cut(s) 51, 848
BmiI GGNNCC 4 cut(s) 52, 319, 415, 910
BmrFI CCNGG 2 cut(s) 146, 712
BmsI GCATC 1 cut(s) 68
BmtI GCTAGC 1 cut(s) 393
BpmI CTGGAG 2 cut(s) 304, 421
Bpu10I CCTNAGC 1 cut(s) 647
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 1 cut(s) 712
BsaI GGTCTC 1 cut(s) 523
BsaJI CCNNGG 2 cut(s) 54, 480
BsaXI ACNNNNNCTCC 2 cut(s) 822, 852
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 486, 918
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 6 cut(s) 301, 321, 404, 724, 782, 923
Bse3DI GCAATG 2 cut(s) 96, 122
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 54, 480
BseGI GGATG 6 cut(s) 59, 126, 329, 335, 579, 737
BseLI CCNNNNNNNGG 3 cut(s) 132, 486, 918
BseMI GCAATG 2 cut(s) 96, 122
BseMII CTCAG 3 cut(s) 93, 298, 638
BseNI ACTGG 6 cut(s) 301, 321, 404, 724, 782, 923
BseXI GCAGC 5 cut(s) 454, 485, 488, 551, 694
BseYI CCCAGC 1 cut(s) 456
BsgI GTGCAG 1 cut(s) 1017
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 125, 510, 1011
BshNI GGYRCC 1 cut(s) 908
BsiHKAI GWGCWC 1 cut(s) 653
BsiSI CCGG 2 cut(s) 315, 712
BslFI GGGAC 3 cut(s) 64, 910, 956
BslI CCNNNNNNNGG 3 cut(s) 132, 486, 918
BsmAI GTCTC 2 cut(s) 523, 790
BsmFI GGGAC 3 cut(s) 64, 910, 956
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 3 cut(s) 125, 510, 1011
Bso31I GGTCTC 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 653
Bsp1407I TGTACA 1 cut(s) 84
Bsp143I GATC 2 cut(s) 433, 584
BspACI CCGC 4 cut(s) 470, 553, 573, 855
BspANI GGCC 3 cut(s) 125, 510, 1011
BspCNI CTCAG 3 cut(s) 94, 297, 639
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 4 cut(s) 52, 319, 415, 910
BspMI ACCTGC 1 cut(s) 989
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 908
BspTNI GGTCTC 1 cut(s) 523
BsrDI GCAATG 2 cut(s) 96, 122
BsrFI RCCGGY 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 84
BsrI ACTGG 6 cut(s) 301, 321, 404, 724, 782, 923
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 54, 480
BssMI GATC 2 cut(s) 433, 584
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 670, 803
BstAPI GCANNNNNTGC 1 cut(s) 467
BstAUI TGTACA 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 312, 316, 391, 705
BstDEI CTNAG 4 cut(s) 102, 284, 647, 977
BstDSI CCRYGG 1 cut(s) 480
BstF5I GGATG 6 cut(s) 59, 126, 329, 335, 579, 737
BstFNI CGCG 1 cut(s) 78
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 2 cut(s) 436, 587
BstMAI GTCTC 2 cut(s) 523, 790
BstMBI GATC 2 cut(s) 433, 584
BstMWI GCNNNNNNNGC 9 cut(s) 65, 98, 467, 473, 476, 529, 561, 570, 1022
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 2 cut(s) 144, 710
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 5 cut(s) 454, 485, 488, 551, 694
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 3 cut(s) 125, 510, 1011
BtgI CCRYGG 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 1031
BtsCI GGATG 6 cut(s) 59, 126, 329, 335, 579, 737
BveI ACCTGC 1 cut(s) 989
Cac8I GCNNGC 4 cut(s) 312, 316, 391, 705
CaiI CAGNNNCTG 1 cut(s) 865
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 51, 848
CseI GACGC 1 cut(s) 235
Csp6I GTAC 4 cut(s) 85, 235, 637, 892
CviAII CATG 8 cut(s) 110, 444, 661, 775, 806, 872, 936, 967
CviQI GTAC 4 cut(s) 85, 235, 637, 892
DdeI CTNAG 4 cut(s) 102, 284, 647, 977
DpnI GATC 2 cut(s) 435, 586
DpnII GATC 2 cut(s) 433, 584
DraI TTTAAA 1 cut(s) 844
EaeI YGGCCR 2 cut(s) 123, 508
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 523
Eco47I GGWCC 2 cut(s) 51, 848
EcoO109I RGGNCCY 2 cut(s) 51, 848
EcoRI GAATTC 2 cut(s) 278, 931
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 8 cut(s) 113, 447, 664, 778, 809, 875, 939, 970
FaqI GGGAC 3 cut(s) 64, 910, 956
FatI CATG 8 cut(s) 109, 443, 660, 774, 805, 871, 935, 966
FauNDI CATATG 1 cut(s) 991
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 6 cut(s) 468, 471, 474, 477, 565, 708
FokI GGATG 6 cut(s) 46, 133, 316, 322, 566, 724
Fsp4HI GCNGC 6 cut(s) 468, 471, 474, 477, 565, 708
FspBI CTAG 2 cut(s) 390, 624
GlaI GCGC 1 cut(s) 79
GluI GCNGC 6 cut(s) 468, 471, 474, 477, 565, 708
GsaI CCCAGC 1 cut(s) 460
GsuI CTGGAG 2 cut(s) 304, 421
HaeIII GGCC 3 cut(s) 125, 510, 1011
HapII CCGG 2 cut(s) 315, 712
HgaI GACGC 1 cut(s) 235
HhaI GCGC 1 cut(s) 80
Hin1II CATG 8 cut(s) 113, 447, 664, 778, 809, 875, 939, 970
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 90
HpaI GTTAAC 1 cut(s) 666
HpaII CCGG 2 cut(s) 315, 712
HphI GGTGA 3 cut(s) 412, 587, 965
Hpy166II GTNNAC 2 cut(s) 142, 666
Hpy188I TCNGA 8 cut(s) 262, 287, 340, 529, 584, 658, 861, 1035
Hpy188III TCNNGA 2 cut(s) 184, 787
Hpy8I GTNNAC 2 cut(s) 142, 666
Hpy99I CGWCG 1 cut(s) 803
HpyAV CCTTC 2 cut(s) 154, 479
HpyCH4III ACNGT 2 cut(s) 670, 803
HpyCH4IV ACGT 2 cut(s) 169, 233
HpyCH4V TGCA 3 cut(s) 703, 778, 998
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 98, 467, 473, 476, 529, 561, 570, 1022
HpyF3I CTNAG 4 cut(s) 102, 284, 647, 977
HpySE526I ACGT 2 cut(s) 169, 233
Hsp92II CATG 8 cut(s) 113, 447, 664, 778, 809, 875, 939, 970
HspAI GCGC 1 cut(s) 78
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
KspAI GTTAAC 1 cut(s) 666
Kzo9I GATC 2 cut(s) 433, 584
LmnI GCTCC 2 cut(s) 323, 413
Lsp1109I GCAGC 5 cut(s) 454, 485, 488, 551, 694
LweI GCATC 1 cut(s) 68
MaeI CTAG 2 cut(s) 390, 624
MaeII ACGT 2 cut(s) 169, 233
MaeIII GTNAC 6 cut(s) 395, 418, 548, 575, 733, 877
MalI GATC 2 cut(s) 435, 586
MboI GATC 2 cut(s) 433, 584
MboII GAAGA 4 cut(s) 199, 287, 353, 641
MfeI CAATTG 1 cut(s) 512
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 9 cut(s) 209, 278, 512, 618, 815, 882, 924, 931, 972
MmeI TCCRAC 1 cut(s) 765
MnlI CCTC 6 cut(s) 293, 343, 396, 433, 608, 824
MroNI GCCGGC 1 cut(s) 314
MseI TTAA 5 cut(s) 326, 429, 665, 843, 927
MspI CCGG 2 cut(s) 315, 712
MspR9I CCNGG 2 cut(s) 146, 712
MunI CAATTG 1 cut(s) 512
Mva1269I GAATGC 1 cut(s) 683
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 9 cut(s) 65, 98, 467, 473, 476, 529, 561, 570, 1022
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 1 cut(s) 712
NdeI CATATG 1 cut(s) 991
NdeII GATC 2 cut(s) 433, 584
NgoMIV GCCGGC 1 cut(s) 314
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 8 cut(s) 113, 447, 664, 778, 809, 875, 939, 970
NlaIV GGNNCC 4 cut(s) 52, 319, 415, 910
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 5 cut(s) 395, 418, 548, 575, 877
NspI RCATGY 1 cut(s) 664
PciI ACATGT 1 cut(s) 660
PctI GAATGC 1 cut(s) 683
PdiI GCCGGC 1 cut(s) 316
PflMI CCANNNNNTGG 1 cut(s) 918
PkrI GCNGC 6 cut(s) 469, 472, 475, 478, 566, 709
PpuMI RGGWCCY 2 cut(s) 51, 848
PscI ACATGT 1 cut(s) 660
Psp5II RGGWCCY 2 cut(s) 51, 848
Psp6I CCWGG 1 cut(s) 144
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 4 cut(s) 52, 319, 415, 910
PspPI GGNCC 2 cut(s) 51, 848
PspPPI RGGWCCY 2 cut(s) 51, 848
PstNI CAGNNNCTG 1 cut(s) 865
RsaI GTAC 4 cut(s) 86, 236, 638, 893
RsaNI GTAC 4 cut(s) 85, 235, 637, 892
SaqAI TTAA 5 cut(s) 326, 429, 665, 843, 927
SatI GCNGC 6 cut(s) 468, 471, 474, 477, 565, 708
Sau3AI GATC 2 cut(s) 433, 584
Sau96I GGNCC 2 cut(s) 51, 848
ScrFI CCNGG 2 cut(s) 146, 712
SduI GDGCHC 1 cut(s) 653
SfaNI GCATC 1 cut(s) 68
SinI GGWCC 2 cut(s) 51, 848
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 9 cut(s) 209, 278, 512, 618, 815, 882, 924, 931, 972
SsiI CCGC 4 cut(s) 470, 553, 573, 855
SspMI CTAG 2 cut(s) 390, 624
StyD4I CCNGG 2 cut(s) 144, 710
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 670, 803
TaiI ACGT 2 cut(s) 172, 236
TaqI TCGA 4 cut(s) 361, 688, 788, 798
TasI AATT 9 cut(s) 209, 278, 512, 618, 815, 882, 924, 931, 972
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 473
Tru1I TTAA 5 cut(s) 326, 429, 665, 843, 927
Tru9I TTAA 5 cut(s) 326, 429, 665, 843, 927
TseFI GTSAC 5 cut(s) 395, 418, 548, 575, 877
TseI GCWGC 5 cut(s) 467, 473, 476, 564, 707
Tsp45I GTSAC 5 cut(s) 395, 418, 548, 575, 877
TspDTI ATGAA 7 cut(s) 28, 288, 432, 509, 741, 924, 983
TspGWI ACGGA 1 cut(s) 843
Van91I CCANNNNNTGG 1 cut(s) 918
VpaK11BI GGWCC 2 cut(s) 51, 848
XapI RAATTY 5 cut(s) 209, 278, 815, 931, 972
XceI RCATGY 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 2 cut(s) 390, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.