Rh5BG461300

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000341
Physical Location & Seq
Forward (+)
73771280 .. 73771915
636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG461300.1

Sequence Viewer

Length: 636 bp
ATGATATATTATCCCTTGTTACTAACTTTTGTATATATACATGTAGGTTCTTGCTGGGCATTTGCAGTAGCAGCAACTGTGGAAGGGCTAACCAAGATCAAAACAGGCCAATTGATCTCACTCTCCGAGCAGCAACTTGTGGACTGCAATCATCAGAACACTGGGTGCAAGGGTGGTTACTTAGAATACGCCTATTCCTACGTAGTACAAAACGGAGGACTCGCTCGAGAAGAAACTTACCCGTACCAGGCAACAGACATGGGAACATGCGATACTAACAAGGAAAGTGAGCATGCGGCACAGATCACTGACTATGAACACGTCCCTTCCAGAAGTGAAAACGATCTACAGAAGGCTGTGGCCATGCAACCAGTATCGGTTACCATTTCTGCTTATGGACAAGAGTTTCAGATGTACGGCAGTGGGGTGTTCTCCGGCAATTGTGGGACGGAGCAAGACCACGCCGTCACGGTCATTGGCTACGGGACGATTGAAGATGGAACCCCCTATTGGTTAATAAAGAACTCATGGGGGCAGAGTTGGGGTGAAAGTGGGTATATGAGAATTCTTAGAAACATTAATGCCCCAGAGGGTATGTGCGGCCTTGCTATCAATCCCCTCTATCCGACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

211

Amino Acids

23.2

Weight (kDa)

4.71

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 16 - 209 2.8e-69 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
AciI CCGC 2 cut(s) 296, 600
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 1 cut(s) 564
AdeI CACNNNGTG 1 cut(s) 165
AfaI GTAC 3 cut(s) 207, 245, 416
AfiI CCNNNNNNNGG 2 cut(s) 247, 510
AflIII ACRYGT 2 cut(s) 40, 319
AgsI TTSAA 1 cut(s) 494
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 493, 525
AlwNI CAGNNNCTG 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 225
AoxI GGCC 3 cut(s) 106, 360, 601
ApeKI GCWGC 2 cut(s) 71, 130
ApoI RAATTY 1 cut(s) 564
AseI ATTAAT 1 cut(s) 579
AsuHPI GGTGA 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 225
BalI TGGCCA 1 cut(s) 362
BbvI GCAGC 2 cut(s) 83, 142
BccI CCATC 1 cut(s) 491
BceAI ACGGC 2 cut(s) 433, 449
BciT130I CCWGG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 347
BisI GCNGC 4 cut(s) 72, 131, 297, 601
BlsI GCNGC 4 cut(s) 73, 132, 298, 602
Bme1390I CCNGG 1 cut(s) 248
BmeT110I CYCGRG 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 1 cut(s) 248
BmrI ACTGGG 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 202
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 510
Bse1I ACTGG 2 cut(s) 166, 371
BseBI CCWGG 1 cut(s) 248
BseLI CCNNNNNNNGG 2 cut(s) 247, 510
BseNI ACTGG 2 cut(s) 166, 371
BseXI GCAGC 2 cut(s) 83, 142
BseYI CCCAGC 1 cut(s) 54
BshFI GGCC 3 cut(s) 108, 362, 603
BsiHKCI CYCGRG 1 cut(s) 225
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 3 cut(s) 308, 460, 499
BslI CCNNNNNNNGG 2 cut(s) 247, 510
BsmFI GGGAC 3 cut(s) 308, 460, 499
BsnI GGCC 3 cut(s) 108, 362, 603
BsoBI CYCGRG 1 cut(s) 225
Bsp143I GATC 4 cut(s) 96, 114, 303, 343
BspACI CCGC 2 cut(s) 296, 600
BspANI GGCC 3 cut(s) 108, 362, 603
BspLI GGNNCC 1 cut(s) 502
BsrI ACTGG 2 cut(s) 166, 371
BssMI GATC 4 cut(s) 96, 114, 303, 343
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 2 cut(s) 79, 472
BstBAI YACGTR 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 181, 569
BstEII GGTNACC 1 cut(s) 379
BstKTI GATC 4 cut(s) 99, 117, 306, 346
BstMBI GATC 4 cut(s) 96, 114, 303, 343
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 3 cut(s) 44, 270, 296
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 347
BstSNI TACGTA 1 cut(s) 202
BstV1I GCAGC 2 cut(s) 83, 142
BsuRI GGCC 3 cut(s) 108, 362, 603
BtrI CACGTC 1 cut(s) 322
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 159, 306, 427
Cac8I GCNNGC 1 cut(s) 294
CaiI CAGNNNCTG 1 cut(s) 77
Csp6I GTAC 3 cut(s) 206, 244, 415
CviAII CATG 6 cut(s) 41, 259, 267, 293, 364, 528
CviJI RGCY 6 cut(s) 88, 108, 356, 362, 480, 603
CviKI_1 RGCY 6 cut(s) 88, 108, 356, 362, 480, 603
CviQI GTAC 3 cut(s) 206, 244, 415
DdeI CTNAG 2 cut(s) 181, 569
DpnI GATC 4 cut(s) 98, 116, 305, 345
DpnII GATC 4 cut(s) 96, 114, 303, 343
DraIII CACNNNGTG 1 cut(s) 165
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 360
Eco105I TACGTA 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 379
EcoO65I GGTNACC 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 564
EcoRII CCWGG 1 cut(s) 246
FaeI CATG 6 cut(s) 44, 262, 270, 296, 367, 531
FaqI GGGAC 3 cut(s) 308, 460, 499
FatI CATG 6 cut(s) 40, 258, 266, 292, 363, 527
Fnu4HI GCNGC 4 cut(s) 72, 131, 297, 601
Fsp4HI GCNGC 4 cut(s) 72, 131, 297, 601
GluI GCNGC 4 cut(s) 72, 131, 297, 601
GsaI CCCAGC 1 cut(s) 58
HaeIII GGCC 3 cut(s) 108, 362, 603
HapII CCGG 1 cut(s) 435
Hin1II CATG 6 cut(s) 44, 262, 270, 296, 367, 531
HinfI GANTC 1 cut(s) 219
HpaII CCGG 1 cut(s) 435
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 4 cut(s) 127, 156, 411, 627
Hpy188III TCNNGA 2 cut(s) 227, 330
Hpy8I GTNNAC 1 cut(s) 142
HpyAV CCTTC 3 cut(s) 77, 336, 346
HpyCH4III ACNGT 2 cut(s) 79, 472
HpyCH4IV ACGT 2 cut(s) 201, 321
HpyCH4V TGCA 5 cut(s) 65, 147, 168, 367, 632
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 2 cut(s) 181, 569
HpySE526I ACGT 2 cut(s) 201, 321
Hsp92II CATG 6 cut(s) 44, 262, 270, 296, 367, 531
Kzo9I GATC 4 cut(s) 96, 114, 303, 343
LmnI GCTCC 1 cut(s) 451
LpnPI CCDG 9 cut(s) 40, 90, 147, 233, 260, 343, 384, 448, 600
Lsp1109I GCAGC 2 cut(s) 83, 142
MaeII ACGT 2 cut(s) 201, 321
MaeIII GTNAC 4 cut(s) 18, 176, 379, 466
MalI GATC 4 cut(s) 98, 116, 305, 345
MboI GATC 4 cut(s) 96, 114, 303, 343
MboII GAAGA 2 cut(s) 242, 506
MfeI CAATTG 2 cut(s) 110, 439
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 3 cut(s) 110, 439, 564
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 1 cut(s) 213
MnlI CCTC 3 cut(s) 209, 583, 629
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
MseI TTAA 2 cut(s) 515, 579
Msp20I TGGCCA 1 cut(s) 362
MspI CCGG 1 cut(s) 435
MspR9I CCNGG 1 cut(s) 248
MunI CAATTG 2 cut(s) 110, 439
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeII GATC 4 cut(s) 96, 114, 303, 343
NlaIII CATG 6 cut(s) 44, 262, 270, 296, 367, 531
NlaIV GGNNCC 1 cut(s) 502
NmuCI GTSAC 1 cut(s) 466
NspI RCATGY 3 cut(s) 44, 270, 296
PaeI GCATGC 1 cut(s) 296
PaeR7I CTCGAG 1 cut(s) 225
PciI ACATGT 1 cut(s) 40
PkrI GCNGC 4 cut(s) 73, 132, 298, 602
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
Ppu21I YACGTR 1 cut(s) 202
PscI ACATGT 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 579
Psp6I CCWGG 1 cut(s) 246
PspEI GGTNACC 1 cut(s) 379
PspFI CCCAGC 1 cut(s) 54
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 502
PsrI GAACNNNNNNTAC 2 cut(s) 256, 288
PstNI CAGNNNCTG 1 cut(s) 77
RsaI GTAC 3 cut(s) 207, 245, 416
RsaNI GTAC 3 cut(s) 206, 244, 415
SaqAI TTAA 2 cut(s) 515, 579
SatI GCNGC 4 cut(s) 72, 131, 297, 601
Sau3AI GATC 4 cut(s) 96, 114, 303, 343
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 248
SetI ASST 3 cut(s) 49, 204, 324
SfcI CTRYAG 1 cut(s) 347
Sfr274I CTCGAG 1 cut(s) 225
SlaI CTCGAG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 225
SmoI CTYRAG 1 cut(s) 225
SnaBI TACGTA 1 cut(s) 202
SphI GCATGC 1 cut(s) 296
Sse9I AATT 3 cut(s) 110, 439, 564
SsiI CCGC 2 cut(s) 296, 600
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 2 cut(s) 79, 472
TaiI ACGT 2 cut(s) 204, 324
TaqI TCGA 1 cut(s) 226
TasI AATT 3 cut(s) 110, 439, 564
TatI WGTACW 1 cut(s) 205
TauI GCSGC 2 cut(s) 299, 603
Tru1I TTAA 2 cut(s) 515, 579
Tru9I TTAA 2 cut(s) 515, 579
TscAI CASTG 3 cut(s) 166, 313, 427
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 71, 130
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 1 cut(s) 330
TspGWI ACGGA 2 cut(s) 228, 464
TspRI CASTG 3 cut(s) 166, 313, 427
VspI ATTAAT 1 cut(s) 579
XapI RAATTY 1 cut(s) 564
XceI RCATGY 3 cut(s) 44, 270, 296
XhoI CTCGAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.