Rh5BG077000

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000341
Physical Location & Seq
Reverse (-)
6453656 .. 6455483
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG077000.1

Sequence Viewer

Length: 1017 bp
ATGGATGTCAAAAGAAACCATGTGTTCATTGCCATATTCATCATCTTAGGGACTGTAGCATCTCAAGCCACATCCCGCCCATTGTATGCTTCTTCCTTTGCTGAAAAACATGAGCAATGGATGGAAAAGTTCGGACGCGTTTACCCGGATAGTGCAGAGAAGGAGAGGCGTTTTGCCATATTCATGCAGAATGTGGAGTATGTGGAGAAGTTCAATAATGAAGGGAACAAAACTTACAAGTTAAGCATCAACGAATTCTCTGACATGACCAATGAAGAGTTCCTGAGGCGTCGTACTGGGTACAAAATGGCCACCAGCTCAAACTCAACCTCATTCAGGTACCAAAGTCTGGCAAGTACTGAAGTTCCAACTAGTGTTGACTGGAGGGAAAAAGGAGCTGTCACACCGATAAAGGATCAAGGAAGCTGCAGTGTTTGCTGGGCCTTTACCGCGGTAGCAGCTGTAGAAGGGCTTACCAAAATCAAAACTGACCAACTGTTCTCTCTGTCTGAGCAACAACTCGTGGATTGTGATGATGGTAACTTTGGCTGTCAACCTGGTTACATAAATGAAGCTTTTGACTACATCAAGCAAAACGGAATTGCACGCGAAGAAAACTATCAATACCTAAATTCCGACAGTGCCACATGCGACACTAGCAAGGCAAATGAGCGCGCTGCTCAGATAACGGGTTATGAAAATGTGCCGACCAATAGTGAAAGTGATCTACTCAAGGCTGTGGCCATGCAACCAGTCTCAGTTGCCATTGACGCCAGCGGAGAAGCCTTTCAGTCATACAGTGGTGGGGTGTTCTCTGGTGATTGTGGTACCAGCCTGAACCATGCTGTAACAGCCGTTGGGTATGGGACGACTGAAGATGGCACAGATTATTGGTTGATGAAGAATTCATGGGGTGAGACTTGGGGTGAAAATGGCTACATAAAAATTCTTAGAAATGCTAATCCCCCATATGGTCTTTGTGGCATTGCCATCCAAGCTCTCTATCCGACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

338

Amino Acids

37.5

Weight (kDa)

4.87

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 2.8e-21 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 122 - 337 5e-78 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 339, 827
AccB1I GGYRCC 2 cut(s) 339, 827
AccB7I CCANNNNNTGG 1 cut(s) 349
AccII CGCG 4 cut(s) 138, 452, 609, 675
AciI CCGC 4 cut(s) 76, 450, 452, 777
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 2 cut(s) 309, 741
AcsI RAATTY 4 cut(s) 254, 631, 904, 945
AcuI CTGAAG 2 cut(s) 381, 894
AcyI GRCGYC 2 cut(s) 289, 771
AfaI GTAC 5 cut(s) 295, 302, 341, 358, 829
AfiI CCNNNNNNNGG 3 cut(s) 336, 349, 971
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 1 cut(s) 214
AhlI ACTAGT 1 cut(s) 371
AjnI CCWGG 1 cut(s) 556
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 6 cut(s) 318, 398, 426, 461, 575, 998
AluI AGCT 6 cut(s) 318, 398, 426, 461, 575, 998
Alw26I GTCTC 2 cut(s) 760, 911
AlwI GGATC 1 cut(s) 423
AoxI GGCC 3 cut(s) 309, 441, 741
ApeKI GCWGC 3 cut(s) 426, 458, 677
ApoI RAATTY 4 cut(s) 254, 631, 904, 945
Asp700I GAANNNNTTC 1 cut(s) 786
Asp718I GGTACC 2 cut(s) 339, 827
AspLEI GCGC 2 cut(s) 675, 677
AspS9I GGNCC 1 cut(s) 441
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 830, 926, 938
AxyI CCTNAGG 1 cut(s) 284
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BalI TGGCCA 2 cut(s) 311, 743
BanI GGYRCC 2 cut(s) 339, 827
BauI CACGAG 1 cut(s) 521
BbvI GCAGC 3 cut(s) 413, 470, 664
BccI CCATC 4 cut(s) 115, 530, 872, 998
BceAI ACGGC 1 cut(s) 839
BciT130I CCWGG 1 cut(s) 558
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 2 cut(s) 760, 911
BcuI ACTAGT 1 cut(s) 371
BfaI CTAG 2 cut(s) 372, 657
BfmI CTRYAG 3 cut(s) 54, 427, 462
BisI GCNGC 3 cut(s) 427, 459, 678
BlsI GCNGC 3 cut(s) 428, 460, 679
BmcAI AGTACT 1 cut(s) 358
Bme1390I CCNGG 2 cut(s) 146, 558
BmgT120I GGNCC 1 cut(s) 441
BmiI GGNNCC 2 cut(s) 341, 829
BmrFI CCNGG 2 cut(s) 146, 558
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 68, 255
BmuI ACTGGG 1 cut(s) 306
BpmI CTGGAG 1 cut(s) 403
BpuEI CTTGAG 2 cut(s) 48, 716
BpuMI CCSGG 1 cut(s) 146
BsaHI GRCGYC 2 cut(s) 289, 771
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 3 cut(s) 336, 349, 971
Bse1I ACTGG 3 cut(s) 301, 386, 752
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 3 cut(s) 27, 122, 984
BseBI CCWGG 1 cut(s) 558
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 4 cut(s) 10, 71, 126, 990
BseLI CCNNNNNNNGG 3 cut(s) 336, 349, 971
BseMI GCAATG 3 cut(s) 27, 122, 984
BseMII CTCAG 4 cut(s) 275, 501, 695, 771
BseNI ACTGG 3 cut(s) 301, 386, 752
BsePI GCGCGC 1 cut(s) 673
BseXI GCAGC 3 cut(s) 413, 470, 664
BseYI CCCAGC 1 cut(s) 438
BsgI GTGCAG 1 cut(s) 174
Bsh1236I CGCG 4 cut(s) 138, 452, 609, 675
BshFI GGCC 3 cut(s) 311, 443, 743
BshNI GGYRCC 2 cut(s) 339, 827
BsiSI CCGG 1 cut(s) 146
BslFI GGGAC 2 cut(s) 64, 880
BslI CCNNNNNNNGG 3 cut(s) 336, 349, 971
BsmAI GTCTC 2 cut(s) 760, 911
BsmFI GGGAC 2 cut(s) 64, 880
BsnI GGCC 3 cut(s) 311, 443, 743
Bsp143I GATC 2 cut(s) 415, 724
BspACI CCGC 4 cut(s) 76, 450, 452, 777
BspANI GGCC 3 cut(s) 311, 443, 743
BspCNI CTCAG 4 cut(s) 276, 502, 694, 770
BspFNI CGCG 4 cut(s) 138, 452, 609, 675
BspLI GGNNCC 2 cut(s) 341, 829
BspMAI CTGCAG 1 cut(s) 431
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 2 cut(s) 339, 827
BsrDI GCAATG 3 cut(s) 27, 122, 984
BsrI ACTGG 3 cut(s) 301, 386, 752
BssECI CCNNGG 1 cut(s) 450
BssHII GCGCGC 1 cut(s) 673
BssMI GATC 2 cut(s) 415, 724
BssNI GRCGYC 2 cut(s) 289, 771
BssSI CACGAG 1 cut(s) 521
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 1 cut(s) 558
Bst4CI ACNGT 4 cut(s) 55, 498, 641, 800
Bst6I CTCTTC 1 cut(s) 270
BstACI GRCGYC 2 cut(s) 289, 771
BstAPI GCANNNNNTGC 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 607, 675, 775
BstDEI CTNAG 6 cut(s) 46, 284, 510, 681, 757, 950
BstDSI CCRYGG 1 cut(s) 450
BstENI CCTNNNNNAGG 1 cut(s) 334
BstF5I GGATG 4 cut(s) 10, 71, 126, 990
BstFNI CGCG 4 cut(s) 138, 452, 609, 675
BstHHI GCGC 2 cut(s) 675, 677
BstKTI GATC 2 cut(s) 418, 727
BstMAI GTCTC 2 cut(s) 760, 911
BstMBI GATC 2 cut(s) 415, 724
BstMWI GCNNNNNNNGC 8 cut(s) 65, 435, 449, 458, 657, 770, 851, 995
BstNI CCWGG 1 cut(s) 558
BstNSI RCATGY 1 cut(s) 651
BstSCI CCNGG 2 cut(s) 144, 556
BstSFI CTRYAG 3 cut(s) 54, 427, 462
BstUI CGCG 4 cut(s) 138, 452, 609, 675
BstV1I GCAGC 3 cut(s) 413, 470, 664
Bsu36I CCTNAGG 1 cut(s) 284
BsuRI GGCC 3 cut(s) 311, 443, 743
BtgI CCRYGG 1 cut(s) 450
BtsCI GGATG 4 cut(s) 10, 71, 126, 990
BtsI GCAGTG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 436, 646, 805
Cac8I GCNNGC 3 cut(s) 607, 675, 775
CfoI GCGC 2 cut(s) 675, 677
Cfr13I GGNCC 1 cut(s) 441
Cfr42I CCGCGG 1 cut(s) 453
CseI GACGC 3 cut(s) 144, 278, 779
CsiI ACCWGGT 1 cut(s) 556
Csp6I GTAC 5 cut(s) 294, 301, 340, 357, 828
CviAII CATG 8 cut(s) 20, 110, 184, 265, 648, 745, 842, 909
CviQI GTAC 5 cut(s) 294, 301, 340, 357, 828
DdeI CTNAG 6 cut(s) 46, 284, 510, 681, 757, 950
DpnI GATC 2 cut(s) 417, 726
DpnII GATC 2 cut(s) 415, 724
EaeI YGGCCR 2 cut(s) 309, 741
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Eco57I CTGAAG 2 cut(s) 381, 894
Eco81I CCTNAGG 1 cut(s) 284
EcoNI CCTNNNNNAGG 1 cut(s) 334
EcoRI GAATTC 2 cut(s) 254, 904
EcoRII CCWGG 1 cut(s) 556
FaeI CATG 8 cut(s) 23, 113, 187, 268, 651, 748, 845, 912
FaqI GGGAC 2 cut(s) 64, 880
FatI CATG 8 cut(s) 19, 109, 183, 264, 647, 744, 841, 908
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 970
Fnu4HI GCNGC 3 cut(s) 427, 459, 678
FokI GGATG 4 cut(s) 17, 58, 133, 977
Fsp4HI GCNGC 3 cut(s) 427, 459, 678
FspBI CTAG 2 cut(s) 372, 657
GlaI GCGC 2 cut(s) 674, 676
GluI GCNGC 3 cut(s) 427, 459, 678
GsaI CCCAGC 1 cut(s) 442
GsuI CTGGAG 1 cut(s) 403
HaeIII GGCC 3 cut(s) 311, 443, 743
HapII CCGG 1 cut(s) 146
HgaI GACGC 3 cut(s) 144, 278, 779
HhaI GCGC 2 cut(s) 675, 677
Hin1I GRCGYC 2 cut(s) 289, 771
Hin1II CATG 8 cut(s) 23, 113, 187, 268, 651, 748, 845, 912
Hin6I GCGC 2 cut(s) 673, 675
HinP1I GCGC 2 cut(s) 673, 675
HincII GTYRAC 2 cut(s) 379, 554
HindII GTYRAC 2 cut(s) 379, 554
HindIII AAGCTT 1 cut(s) 573
HpaII CCGG 1 cut(s) 146
HphI GGTGA 3 cut(s) 830, 926, 938
Hpy166II GTNNAC 3 cut(s) 142, 379, 554
Hpy188I TCNGA 6 cut(s) 134, 262, 511, 637, 684, 1008
Hpy188III TCNNGA 1 cut(s) 283
Hpy8I GTNNAC 3 cut(s) 142, 379, 554
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 3 cut(s) 154, 215, 461
HpyCH4III ACNGT 4 cut(s) 55, 498, 641, 800
HpyCH4V TGCA 6 cut(s) 155, 187, 429, 605, 748, 1013
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 435, 449, 458, 657, 770, 851, 995
HpyF3I CTNAG 6 cut(s) 46, 284, 510, 681, 757, 950
Hsp92I GRCGYC 2 cut(s) 289, 771
Hsp92II CATG 8 cut(s) 23, 113, 187, 268, 651, 748, 845, 912
HspAI GCGC 2 cut(s) 673, 675
KpnI GGTACC 2 cut(s) 343, 831
KspI CCGCGG 1 cut(s) 453
Kzo9I GATC 2 cut(s) 415, 724
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 3 cut(s) 413, 470, 664
LweI GCATC 2 cut(s) 68, 255
MabI ACCWGGT 1 cut(s) 556
MaeI CTAG 2 cut(s) 372, 657
MaeIII GTNAC 4 cut(s) 400, 539, 560, 847
MalI GATC 2 cut(s) 417, 726
MboI GATC 2 cut(s) 415, 724
MboII GAAGA 5 cut(s) 84, 287, 623, 887, 913
MlsI TGGCCA 2 cut(s) 311, 743
MluCI AATT 5 cut(s) 254, 600, 631, 904, 945
MluI ACGCGT 1 cut(s) 136
MluNI TGGCCA 2 cut(s) 311, 743
MmeI TCCRAC 2 cut(s) 392, 660
MnlI CCTC 4 cut(s) 159, 279, 340, 378
Mox20I TGGCCA 2 cut(s) 311, 743
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 2 cut(s) 311, 743
MseI TTAA 1 cut(s) 242
MslI CAYNNNNRTG 1 cut(s) 182
Msp20I TGGCCA 2 cut(s) 311, 743
MspA1I CMGCKG 3 cut(s) 452, 461, 777
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 146, 558
MvaI CCWGG 1 cut(s) 558
MvnI CGCG 4 cut(s) 138, 452, 609, 675
MwoI GCNNNNNNNGC 8 cut(s) 65, 435, 449, 458, 657, 770, 851, 995
NciI CCSGG 1 cut(s) 146
NdeI CATATG 1 cut(s) 970
NdeII GATC 2 cut(s) 415, 724
NlaIII CATG 8 cut(s) 23, 113, 187, 268, 651, 748, 845, 912
NlaIV GGNNCC 2 cut(s) 341, 829
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 651
PauI GCGCGC 1 cut(s) 673
PdmI GAANNNNTTC 1 cut(s) 786
PflMI CCANNNNNTGG 1 cut(s) 349
PkrI GCNGC 3 cut(s) 428, 460, 679
Psp6I CCWGG 1 cut(s) 556
PspFI CCCAGC 1 cut(s) 438
PspGI CCWGG 1 cut(s) 556
PspN4I GGNNCC 2 cut(s) 341, 829
PspPI GGNCC 1 cut(s) 441
PsrI GAACNNNNNNTAC 2 cut(s) 218, 250
PstI CTGCAG 1 cut(s) 431
PteI GCGCGC 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 5 cut(s) 295, 302, 341, 358, 829
RsaNI GTAC 5 cut(s) 294, 301, 340, 357, 828
RseI CAYNNNNRTG 1 cut(s) 182
SacII CCGCGG 1 cut(s) 453
SaqAI TTAA 1 cut(s) 242
SatI GCNGC 3 cut(s) 427, 459, 678
Sau3AI GATC 2 cut(s) 415, 724
Sau96I GGNCC 1 cut(s) 441
ScaI AGTACT 1 cut(s) 358
ScrFI CCNGG 2 cut(s) 146, 558
SexAI ACCWGGT 1 cut(s) 556
SfaNI GCATC 2 cut(s) 68, 255
SfcI CTRYAG 3 cut(s) 54, 427, 462
Sfr303I CCGCGG 1 cut(s) 453
SgrBI CCGCGG 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 63, 731
SmoI CTYRAG 2 cut(s) 63, 731
SpeI ACTAGT 1 cut(s) 371
Sse9I AATT 5 cut(s) 254, 600, 631, 904, 945
SsiI CCGC 4 cut(s) 76, 450, 452, 777
SspMI CTAG 2 cut(s) 372, 657
StyD4I CCNGG 2 cut(s) 144, 556
TaaI ACNGT 4 cut(s) 55, 498, 641, 800
TasI AATT 5 cut(s) 254, 600, 631, 904, 945
TatI WGTACW 1 cut(s) 356
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 3 cut(s) 436, 646, 805
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 3 cut(s) 426, 458, 677
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 9 cut(s) 16, 28, 172, 234, 288, 585, 711, 897, 914
TspGWI ACGGA 1 cut(s) 612
TspRI CASTG 3 cut(s) 436, 646, 805
Van91I CCANNNNNTGG 1 cut(s) 349
XagI CCTNNNNNAGG 1 cut(s) 334
XapI RAATTY 4 cut(s) 254, 631, 904, 945
XceI RCATGY 1 cut(s) 651
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 2 cut(s) 372, 657
ZrmI AGTACT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.