MD10G1281800.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000341
Physical Location & Seq
Forward (+)
37254747 .. 37255955
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1281800.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCTTTCAAAAGAAACCTTTTGATTGTTACCATACTCATCACCTTGGGGACCTTGGCATCTGAAGCCACGTCCCGCACATTGTACCAAGCTTCCATTGCTGCAAAACATGAGCAGTGGATGAAAAAGCATGGACGCGTTTACCAGGACAGCGTGGAGAAGGAAAGGCGTTTCCACATATTCAAAAACAATGTGGAGTTTGTCGAAAAATTCAACAGTGAAGGGAACATGACTTACAGCCTAGGCATCAACCGATTTTCAGATATGACCTATGACGAATTCCTCAGGCATTATACAGGATACAAGCAGCCCACTGAATCAACCTCATCCGCAGATGCATCATTTACGTACGAAAACCTAAGCCCGACAGACGTTCCCCAAAGCATTGACTGGAGGGAGAATGGAGCTGTTACTCCAGTAAAAGATCAAGGGCGCTGTGGTTGTTGCTGGGCATTTTCTGCAGTGACAGCAGTCGAAGGGATTATCCAAATCAAAACTGACAAATTGATCTCACTGTCTGAGCAACAACTTGTGAACTGTGATACAGATAACAATGGCTGCCAAGGTGGTTGGATGGTTAATGCCTTTGCATACATTCAACGAAACAGAGGAATTACCAGTGAAGAAAATTACCCATACCAGAGTCTAGAAGGAACATGTGATACGAACAAGGAAAATGAGGCTGCTGCCCAGATAACTAGTTATGCAGAGGTACCTCCCAACAGTGAAACCGATCTACTAAAGGCTGTGTCCATGCAACCAGTCTCCGTTTCCATCCATGCCTCACCAGCCTTTCAGCATTATTCAGGTGGGGTGTTCACCGGTGATGATTGTGGGACAGAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCACCCCCCATTGGTTAATCAAGAATTCATGGTCCGATACGTGGGGTGAGAATGGCTACATGAAGATTCTTAGAAACGCTGATGCCCCAGGTGGTGTTTGTGGCCTTGCTATAAGACCTTTCTATCCGACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

342

Amino Acids

37.95

Weight (kDa)

5.15

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 4.9e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 339 1.8e-81 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 712
AccB1I GGYRCC 2 cut(s) 712, 890
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 76, 330
AcsI RAATTY 3 cut(s) 209, 278, 913
AcuI CTGAAG 2 cut(s) 84, 903
AfaI GTAC 3 cut(s) 86, 350, 714
AfiI CCNNNNNNNGG 3 cut(s) 867, 900, 930
AflIII ACRYGT 2 cut(s) 136, 656
AgeI ACCGGT 1 cut(s) 821
AgsI TTSAA 5 cut(s) 10, 184, 214, 599, 847
AhlI ACTAGT 1 cut(s) 698
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 2 cut(s) 144, 976
AluBI AGCT 2 cut(s) 92, 407
AluI AGCT 2 cut(s) 92, 407
Alw26I GTCTC 1 cut(s) 769
AoxI GGCC 1 cut(s) 991
ApeKI GCWGC 5 cut(s) 101, 307, 558, 683, 686
ApoI RAATTY 3 cut(s) 209, 278, 913
AsiGI ACCGGT 1 cut(s) 821
Asp718I GGTACC 1 cut(s) 712
AspA2I CCTAGG 1 cut(s) 241
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 2 cut(s) 51, 921
AsuHPI GGTGA 5 cut(s) 34, 777, 811, 836, 947
AvaII GGWCC 2 cut(s) 51, 921
AvrII CCTAGG 1 cut(s) 241
AxyI CCTNAGG 1 cut(s) 284
BanI GGYRCC 2 cut(s) 712, 890
BbvI GCAGC 5 cut(s) 88, 319, 545, 670, 673
BccI CCATC 4 cut(s) 568, 782, 869, 881
BciT130I CCWGG 2 cut(s) 146, 978
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 769
BcuI ACTAGT 1 cut(s) 698
BfaI CTAG 3 cut(s) 242, 647, 699
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 436
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 5 cut(s) 102, 308, 559, 684, 687
BlnI CCTAGG 1 cut(s) 241
BlsI GCNGC 5 cut(s) 103, 309, 560, 685, 688
Bme1390I CCNGG 2 cut(s) 146, 978
Bme18I GGWCC 2 cut(s) 51, 921
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 2 cut(s) 51, 921
BmiI GGNNCC 3 cut(s) 52, 714, 892
BmrFI CCNGG 2 cut(s) 146, 978
BmsI GCATC 5 cut(s) 68, 255, 325, 347, 961
BoxI GACNNNNGTC 1 cut(s) 470
BpmI CTGGAG 2 cut(s) 399, 412
Bpu10I CCTNAGC 1 cut(s) 359
BsaAI YACGTR 2 cut(s) 348, 930
BsaJI CCNNGG 5 cut(s) 45, 54, 241, 562, 976
BsaWI WCCGGW 1 cut(s) 821
Bsc4I CCNNNNNNNGG 3 cut(s) 867, 900, 930
Bse118I RCCGGY 1 cut(s) 821
Bse1I ACTGG 4 cut(s) 395, 416, 618, 761
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 2 cut(s) 146, 978
BseDI CCNNGG 5 cut(s) 45, 54, 241, 562, 976
BseGI GGATG 4 cut(s) 126, 326, 579, 774
BseLI CCNNNNNNNGG 3 cut(s) 867, 900, 930
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 298, 510
BseNI ACTGG 4 cut(s) 395, 416, 618, 761
BseXI GCAGC 5 cut(s) 88, 319, 545, 670, 673
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 993
BshNI GGYRCC 2 cut(s) 712, 890
BshTI ACCGGT 1 cut(s) 821
BsiSI CCGG 1 cut(s) 822
BsiWI CGTACG 1 cut(s) 348
BslFI GGGAC 4 cut(s) 58, 64, 850, 889
BslI CCNNNNNNNGG 3 cut(s) 867, 900, 930
BsmAI GTCTC 1 cut(s) 769
BsmFI GGGAC 4 cut(s) 58, 64, 850, 889
BsnI GGCC 1 cut(s) 993
Bsp143I GATC 3 cut(s) 424, 507, 733
BspACI CCGC 2 cut(s) 76, 330
BspANI GGCC 1 cut(s) 993
BspCNI CTCAG 2 cut(s) 297, 511
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 52, 714, 892
BspMAI CTGCAG 1 cut(s) 463
BspT107I GGYRCC 2 cut(s) 712, 890
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 821
BsrI ACTGG 4 cut(s) 395, 416, 618, 761
BssAI RCCGGY 1 cut(s) 821
BssECI CCNNGG 5 cut(s) 45, 54, 241, 562, 976
BssMI GATC 3 cut(s) 424, 507, 733
BssT1I CCWWGG 4 cut(s) 45, 54, 241, 562
Bst2UI CCWGG 2 cut(s) 146, 978
Bst4CI ACNGT 4 cut(s) 218, 516, 539, 725
BstAPI GCANNNNNTGC 1 cut(s) 458
BstBAI YACGTR 2 cut(s) 348, 930
BstDEI CTNAG 4 cut(s) 284, 359, 519, 959
BstF5I GGATG 4 cut(s) 126, 326, 579, 774
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 3 cut(s) 427, 510, 736
BstMAI GTCTC 1 cut(s) 769
BstMBI GATC 3 cut(s) 424, 507, 733
BstMWI GCNNNNNNNGC 5 cut(s) 65, 98, 458, 467, 788
BstNI CCWGG 2 cut(s) 146, 978
BstNSI RCATGY 1 cut(s) 660
BstPAI GACNNNNGTC 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 144, 976
BstSFI CTRYAG 1 cut(s) 459
BstSNI TACGTA 1 cut(s) 348
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 5 cut(s) 88, 319, 545, 670, 673
Bsu36I CCTNAGG 1 cut(s) 284
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 993
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 4 cut(s) 126, 326, 579, 774
BtsI GCAGTG 2 cut(s) 122, 468
BtsIMutI CAGTG 7 cut(s) 122, 223, 312, 468, 512, 625, 730
CfoI GCGC 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 821
Cfr13I GGNCC 2 cut(s) 51, 921
CseI GACGC 1 cut(s) 144
Csp6I GTAC 3 cut(s) 85, 349, 713
CspAI ACCGGT 1 cut(s) 821
CviAII CATG 8 cut(s) 110, 131, 229, 657, 754, 779, 918, 949
CviQI GTAC 3 cut(s) 85, 349, 713
DdeI CTNAG 4 cut(s) 284, 359, 519, 959
DpnI GATC 3 cut(s) 426, 509, 735
DpnII GATC 3 cut(s) 424, 507, 733
Eco105I TACGTA 1 cut(s) 348
Eco130I CCWWGG 4 cut(s) 45, 54, 241, 562
Eco47I GGWCC 2 cut(s) 51, 921
Eco57I CTGAAG 2 cut(s) 84, 903
Eco81I CCTNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 278, 913
EcoRII CCWGG 2 cut(s) 144, 976
EcoT14I CCWWGG 4 cut(s) 45, 54, 241, 562
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 4 cut(s) 45, 54, 241, 562
FaeI CATG 8 cut(s) 113, 134, 232, 660, 757, 782, 921, 952
FaqI GGGAC 4 cut(s) 58, 64, 850, 889
FatI CATG 8 cut(s) 109, 130, 228, 656, 753, 778, 917, 948
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 5 cut(s) 102, 308, 559, 684, 687
FokI GGATG 4 cut(s) 133, 313, 586, 761
Fsp4HI GCNGC 5 cut(s) 102, 308, 559, 684, 687
FspBI CTAG 3 cut(s) 242, 647, 699
GlaI GCGC 1 cut(s) 434
GluI GCNGC 5 cut(s) 102, 308, 559, 684, 687
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 2 cut(s) 399, 412
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 1 cut(s) 993
HapII CCGG 1 cut(s) 822
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 435
Hin1II CATG 8 cut(s) 113, 134, 232, 660, 757, 782, 921, 952
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 3 cut(s) 317, 643, 955
HpaII CCGG 1 cut(s) 822
HphI GGTGA 5 cut(s) 34, 777, 811, 836, 947
Hpy166II GTNNAC 3 cut(s) 142, 535, 819
Hpy188I TCNGA 5 cut(s) 64, 262, 520, 925, 1017
Hpy188III TCNNGA 2 cut(s) 647, 910
Hpy8I GTNNAC 3 cut(s) 142, 535, 819
HpyAV CCTTC 4 cut(s) 154, 215, 470, 644
HpyCH4III ACNGT 4 cut(s) 218, 516, 539, 725
HpyCH4IV ACGT 4 cut(s) 71, 347, 372, 929
HpyCH4V TGCA 6 cut(s) 104, 338, 461, 590, 707, 757
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 98, 458, 467, 788
HpyF3I CTNAG 4 cut(s) 284, 359, 519, 959
HpySE526I ACGT 4 cut(s) 71, 347, 372, 929
Hsp92II CATG 8 cut(s) 113, 134, 232, 660, 757, 782, 921, 952
HspAI GCGC 1 cut(s) 433
KpnI GGTACC 1 cut(s) 716
Kzo9I GATC 3 cut(s) 424, 507, 733
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 5 cut(s) 88, 319, 545, 670, 673
LweI GCATC 5 cut(s) 68, 255, 325, 347, 961
MaeI CTAG 3 cut(s) 242, 647, 699
MaeII ACGT 4 cut(s) 71, 347, 372, 929
MaeIII GTNAC 4 cut(s) 28, 409, 463, 856
MalI GATC 3 cut(s) 426, 509, 735
MboI GATC 3 cut(s) 424, 507, 733
MboII GAAGA 3 cut(s) 635, 896, 964
MluCI AATT 7 cut(s) 209, 278, 503, 612, 628, 842, 913
MluI ACGCGT 1 cut(s) 136
MlyI GAGTC 1 cut(s) 652
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 8 cut(s) 293, 334, 387, 602, 673, 703, 726, 793
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 579, 905
MspI CCGG 1 cut(s) 822
MspR9I CCNGG 2 cut(s) 146, 978
MvaI CCWGG 2 cut(s) 146, 978
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 5 cut(s) 65, 98, 458, 467, 788
NdeII GATC 3 cut(s) 424, 507, 733
NlaIII CATG 8 cut(s) 113, 134, 232, 660, 757, 782, 921, 952
NlaIV GGNNCC 3 cut(s) 52, 714, 892
NmuCI GTSAC 1 cut(s) 463
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 660
PciI ACATGT 1 cut(s) 656
PfeI GAWTC 2 cut(s) 317, 955
Pfl23II CGTACG 1 cut(s) 348
PinAI ACCGGT 1 cut(s) 821
PkrI GCNGC 5 cut(s) 103, 309, 560, 685, 688
PleI GAGTC 1 cut(s) 651
PpsI GAGTC 1 cut(s) 651
Ppu21I YACGTR 2 cut(s) 348, 930
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 656
PshAI GACNNNNGTC 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 144, 976
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 2 cut(s) 144, 976
PspLI CGTACG 1 cut(s) 348
PspN4I GGNNCC 3 cut(s) 52, 714, 892
PspPI GGNCC 2 cut(s) 51, 921
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 4 cut(s) 218, 250, 646, 678
PstI CTGCAG 1 cut(s) 463
RsaI GTAC 3 cut(s) 86, 350, 714
RsaNI GTAC 3 cut(s) 85, 349, 713
SaqAI TTAA 2 cut(s) 579, 905
SatI GCNGC 5 cut(s) 102, 308, 559, 684, 687
Sau3AI GATC 3 cut(s) 424, 507, 733
Sau96I GGNCC 2 cut(s) 51, 921
SchI GAGTC 1 cut(s) 652
ScrFI CCNGG 2 cut(s) 146, 978
SfaNI GCATC 5 cut(s) 68, 255, 325, 347, 961
SfcI CTRYAG 1 cut(s) 459
SgrAI CRCCGGYG 1 cut(s) 821
SinI GGWCC 2 cut(s) 51, 921
SnaBI TACGTA 1 cut(s) 348
SpeI ACTAGT 1 cut(s) 698
Sse9I AATT 7 cut(s) 209, 278, 503, 612, 628, 842, 913
SsiI CCGC 2 cut(s) 76, 330
SspMI CTAG 3 cut(s) 242, 647, 699
StyD4I CCNGG 2 cut(s) 144, 976
StyI CCWWGG 4 cut(s) 45, 54, 241, 562
TaaI ACNGT 4 cut(s) 218, 516, 539, 725
TaiI ACGT 4 cut(s) 74, 350, 375, 932
TaqI TCGA 2 cut(s) 204, 474
TasI AATT 7 cut(s) 209, 278, 503, 612, 628, 842, 913
TfiI GAWTC 2 cut(s) 317, 955
Tru1I TTAA 2 cut(s) 579, 905
Tru9I TTAA 2 cut(s) 579, 905
TscAI CASTG 7 cut(s) 122, 223, 319, 468, 519, 625, 730
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 5 cut(s) 101, 307, 558, 683, 686
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 3 cut(s) 137, 906, 965
TspGWI ACGGA 1 cut(s) 757
TspRI CASTG 7 cut(s) 122, 223, 319, 468, 519, 625, 730
VpaK11BI GGWCC 2 cut(s) 51, 921
XapI RAATTY 3 cut(s) 209, 278, 913
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 1 cut(s) 660
XmaJI CCTAGG 1 cut(s) 241
XspI CTAG 3 cut(s) 242, 647, 699
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.