MD10G1281700.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000341
Physical Location & Seq
Forward (+)
37235970 .. 37237174
1205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1281700.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGCTTTTGAAAGAAACCTTTTGATTGTTACCATACTCATCACCTTGGGGACCTTGGCATCTGAAGCCACGTCCCGCACATTGTACCAAGCTTCCATTGCTGCAAAACATGAGCAGTGGATGAAAAAGCATGGACGCGTTTACCAGGACAGCGCAGAGAAGGAAAGGCGTTTCCAGATATTCAAAAACAATGTGGAGTTTGTCGAGAAATTCAACAGTGAAGGGAACATGACTTACAGCCTATCCATCAACCGATTTTCGGATATGACCTATGACGAATTCCTCAGGCATTATACTGGATACAAGCAGCCCACTGAATCAACCTCATCTGCTGCTGCATCTTTTACATACGAAAACCTAAGCCCGGCTGATGTTCCCCAAAGCATTGACTGGAGGGAGAAGAGTGCTGTTACTCCAGTAAAATTTCAAGGGAGCTGTGGTTGTTGCTGGGCATTTGCCGCAGTGGCAGCAGTCGAAGGGATTACCCAAATCAAAACTGGCAAATTGATCTCACTGTCTGAGCAACAACTTGTGAACTGTGATACAGAAATTAACGAGGGCTGCCAAGGTGGTCGGGCAGTTAATGCCTTTGCATACATTCAACGAAACGGAGGAATCACCAGTGAAGAAAACTACCCATACCAGGGTTTAGATGGCACATGTGATATGAACAAGGCAAATGAGGCTGCTGCCCACATAACTGGTTATGCAGAGGTACCTCCCAACAGTGAAACCGATCTACTCAAGGCTGTGTCCATGCAACCAGTCTCCGTTTCCATCCACGCCTCACCAGCCTTTCAGCATTATTCAGGCGGGGTGTTCACCGGTGATGATTGTGGGACAAAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCACCCCCTATTGGTTAATCAAGAATTCATGGTCTGATACGTGGGGTGAGAATGGCTACATGAAGATTCTTAGAAACGCTGATGCCCCAGGTGGTGTTTGTGGCCTCGCTATAAGACCTTGCTATCCGACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

343

Amino Acids

37.61

Weight (kDa)

5.34

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 4.5e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 340 5e-80 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 715
AccB1I GGYRCC 2 cut(s) 715, 893
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 76, 459, 813
AcsI RAATTY 4 cut(s) 209, 278, 422, 916
AcuI CTGAAG 2 cut(s) 84, 906
AfaI GTAC 2 cut(s) 86, 717
AfiI CCNNNNNNNGG 6 cut(s) 259, 364, 643, 644, 870, 903
AflIII ACRYGT 2 cut(s) 136, 659
AgeI ACCGGT 1 cut(s) 824
AgsI TTSAA 6 cut(s) 11, 184, 214, 428, 602, 850
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 3 cut(s) 144, 642, 979
AluBI AGCT 2 cut(s) 92, 435
AluI AGCT 2 cut(s) 92, 435
Alw26I GTCTC 1 cut(s) 772
AoxI GGCC 1 cut(s) 994
ApeKI GCWGC 8 cut(s) 101, 307, 332, 335, 467, 561, 686, 689
ApoI RAATTY 4 cut(s) 209, 278, 422, 916
AsiGI ACCGGT 1 cut(s) 824
Asp718I GGTACC 1 cut(s) 715
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 51
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 6 cut(s) 34, 610, 780, 814, 839, 950
AvaII GGWCC 1 cut(s) 51
AxyI CCTNAGG 1 cut(s) 284
BanI GGYRCC 2 cut(s) 715, 893
BbvI GCAGC 8 cut(s) 88, 319, 319, 322, 479, 548, 673, 676
BccI CCATC 5 cut(s) 254, 647, 785, 872, 884
BciT130I CCWGG 3 cut(s) 146, 644, 981
BciVI GTATCC 1 cut(s) 293
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 1 cut(s) 772
BfuI GTATCC 1 cut(s) 293
BglI GCCNNNNNGGC 1 cut(s) 464
BisI GCNGC 9 cut(s) 102, 308, 333, 336, 459, 468, 562, 687, 690
BlsI GCNGC 9 cut(s) 103, 309, 334, 337, 460, 469, 563, 688, 691
Bme1390I CCNGG 4 cut(s) 146, 365, 644, 981
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 3 cut(s) 52, 717, 895
BmrFI CCNGG 4 cut(s) 146, 365, 644, 981
BmsI GCATC 3 cut(s) 68, 347, 964
BpmI CTGGAG 2 cut(s) 399, 412
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 728
BpuMI CCSGG 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 933
BsaJI CCNNGG 5 cut(s) 45, 54, 565, 643, 979
BsaWI WCCGGW 1 cut(s) 824
BsaXI ACNNNNNCTCC 2 cut(s) 424, 454
Bsc4I CCNNNNNNNGG 6 cut(s) 259, 364, 643, 644, 870, 903
Bse118I RCCGGY 1 cut(s) 824
Bse1I ACTGG 7 cut(s) 301, 395, 416, 502, 621, 706, 764
Bse21I CCTNAGG 1 cut(s) 284
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 3 cut(s) 146, 644, 981
BseDI CCNNGG 5 cut(s) 45, 54, 565, 643, 979
BseGI GGATG 2 cut(s) 126, 777
BseLI CCNNNNNNNGG 6 cut(s) 259, 364, 643, 644, 870, 903
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 298, 510
BseNI ACTGG 7 cut(s) 301, 395, 416, 502, 621, 706, 764
BseXI GCAGC 8 cut(s) 88, 319, 319, 322, 479, 548, 673, 676
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 1 cut(s) 996
BshNI GGYRCC 2 cut(s) 715, 893
BshTI ACCGGT 1 cut(s) 824
BsiSI CCGG 2 cut(s) 365, 825
BslFI GGGAC 4 cut(s) 58, 64, 853, 892
BslI CCNNNNNNNGG 6 cut(s) 259, 364, 643, 644, 870, 903
BsmAI GTCTC 1 cut(s) 772
BsmFI GGGAC 4 cut(s) 58, 64, 853, 892
BsnI GGCC 1 cut(s) 996
Bsp143I GATC 2 cut(s) 507, 736
BspACI CCGC 3 cut(s) 76, 459, 813
BspANI GGCC 1 cut(s) 996
BspCNI CTCAG 2 cut(s) 297, 511
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 52, 717, 895
BspT107I GGYRCC 2 cut(s) 715, 893
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 824
BsrI ACTGG 7 cut(s) 301, 395, 416, 502, 621, 706, 764
BssAI RCCGGY 1 cut(s) 824
BssECI CCNNGG 5 cut(s) 45, 54, 565, 643, 979
BssMI GATC 2 cut(s) 507, 736
BssT1I CCWWGG 3 cut(s) 45, 54, 565
Bst2UI CCWGG 3 cut(s) 146, 644, 981
Bst4CI ACNGT 4 cut(s) 218, 516, 539, 728
Bst6I CTCTTC 1 cut(s) 395
BstAPI GCANNNNNTGC 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 933
BstDEI CTNAG 4 cut(s) 284, 359, 519, 962
BstF5I GGATG 2 cut(s) 126, 777
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 2 cut(s) 510, 739
BstMAI GTCTC 1 cut(s) 772
BstMBI GATC 2 cut(s) 507, 736
BstMWI GCNNNNNNNGC 8 cut(s) 65, 98, 458, 464, 467, 584, 683, 791
BstNI CCWGG 3 cut(s) 146, 644, 981
BstNSI RCATGY 1 cut(s) 663
BstSCI CCNGG 4 cut(s) 144, 363, 642, 979
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 8 cut(s) 88, 319, 319, 322, 479, 548, 673, 676
BstXI CCANNNNNNTGG 1 cut(s) 701
Bsu36I CCTNAGG 1 cut(s) 284
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 996
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 2 cut(s) 126, 777
BtsI GCAGTG 2 cut(s) 122, 468
BtsIMutI CAGTG 7 cut(s) 122, 223, 312, 468, 512, 628, 733
CfoI GCGC 1 cut(s) 155
Cfr10I RCCGGY 1 cut(s) 824
Cfr13I GGNCC 1 cut(s) 51
CseI GACGC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 85, 716
CspAI ACCGGT 1 cut(s) 824
CviAII CATG 7 cut(s) 110, 131, 229, 660, 757, 921, 952
CviQI GTAC 2 cut(s) 85, 716
DdeI CTNAG 4 cut(s) 284, 359, 519, 962
DpnI GATC 2 cut(s) 509, 738
DpnII GATC 2 cut(s) 507, 736
Eam1104I CTCTTC 1 cut(s) 395
EarI CTCTTC 1 cut(s) 395
Eco130I CCWWGG 3 cut(s) 45, 54, 565
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 84, 906
Eco81I CCTNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 2 cut(s) 278, 916
EcoRII CCWGG 3 cut(s) 144, 642, 979
EcoT14I CCWWGG 3 cut(s) 45, 54, 565
ErhI CCWWGG 3 cut(s) 45, 54, 565
FaeI CATG 7 cut(s) 113, 134, 232, 663, 760, 924, 955
FaqI GGGAC 4 cut(s) 58, 64, 853, 892
FatI CATG 7 cut(s) 109, 130, 228, 659, 756, 920, 951
FauI CCCGC 2 cut(s) 83, 806
Fnu4HI GCNGC 9 cut(s) 102, 308, 333, 336, 459, 468, 562, 687, 690
FokI GGATG 2 cut(s) 133, 764
Fsp4HI GCNGC 9 cut(s) 102, 308, 333, 336, 459, 468, 562, 687, 690
GlaI GCGC 1 cut(s) 154
GluI GCNGC 9 cut(s) 102, 308, 333, 336, 459, 468, 562, 687, 690
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 2 cut(s) 399, 412
HaeIII GGCC 1 cut(s) 996
HapII CCGG 2 cut(s) 365, 825
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 155
Hin1II CATG 7 cut(s) 113, 134, 232, 663, 760, 924, 955
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 3 cut(s) 317, 615, 958
HpaII CCGG 2 cut(s) 365, 825
HphI GGTGA 6 cut(s) 34, 610, 780, 814, 839, 950
Hpy166II GTNNAC 3 cut(s) 142, 535, 822
Hpy188I TCNGA 5 cut(s) 64, 262, 520, 928, 1020
Hpy188III TCNNGA 3 cut(s) 175, 205, 913
Hpy8I GTNNAC 3 cut(s) 142, 535, 822
HpyAV CCTTC 3 cut(s) 154, 215, 470
HpyCH4III ACNGT 4 cut(s) 218, 516, 539, 728
HpyCH4IV ACGT 2 cut(s) 71, 932
HpyCH4V TGCA 5 cut(s) 104, 338, 593, 710, 760
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 98, 458, 464, 467, 584, 683, 791
HpyF3I CTNAG 4 cut(s) 284, 359, 519, 962
HpySE526I ACGT 2 cut(s) 71, 932
Hsp92II CATG 7 cut(s) 113, 134, 232, 663, 760, 924, 955
HspAI GCGC 1 cut(s) 153
KpnI GGTACC 1 cut(s) 719
Kzo9I GATC 2 cut(s) 507, 736
LmnI GCTCC 1 cut(s) 432
Lsp1109I GCAGC 8 cut(s) 88, 319, 319, 322, 479, 548, 673, 676
LweI GCATC 3 cut(s) 68, 347, 964
MaeII ACGT 2 cut(s) 71, 932
MaeIII GTNAC 3 cut(s) 28, 409, 859
MalI GATC 2 cut(s) 509, 738
MboI GATC 2 cut(s) 507, 736
MboII GAAGA 4 cut(s) 412, 638, 899, 967
MluCI AATT 7 cut(s) 209, 278, 422, 503, 549, 845, 916
MluI ACGCGT 1 cut(s) 136
MseI TTAA 3 cut(s) 552, 582, 908
MspI CCGG 2 cut(s) 365, 825
MspR9I CCNGG 4 cut(s) 146, 365, 644, 981
MvaI CCWGG 3 cut(s) 146, 644, 981
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 8 cut(s) 65, 98, 458, 464, 467, 584, 683, 791
NciI CCSGG 1 cut(s) 365
NdeII GATC 2 cut(s) 507, 736
NlaIII CATG 7 cut(s) 113, 134, 232, 663, 760, 924, 955
NlaIV GGNNCC 3 cut(s) 52, 717, 895
NspI RCATGY 1 cut(s) 663
PciI ACATGT 1 cut(s) 659
PfeI GAWTC 3 cut(s) 317, 615, 958
PinAI ACCGGT 1 cut(s) 824
PkrI GCNGC 9 cut(s) 103, 309, 334, 337, 460, 469, 563, 688, 691
Ppu21I YACGTR 1 cut(s) 933
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 659
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 144, 642, 979
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 3 cut(s) 144, 642, 979
PspN4I GGNNCC 3 cut(s) 52, 717, 895
PspPI GGNCC 1 cut(s) 51
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 218, 250
RsaI GTAC 2 cut(s) 86, 717
RsaNI GTAC 2 cut(s) 85, 716
SaqAI TTAA 3 cut(s) 552, 582, 908
SatI GCNGC 9 cut(s) 102, 308, 333, 336, 459, 468, 562, 687, 690
Sau3AI GATC 2 cut(s) 507, 736
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 4 cut(s) 146, 365, 644, 981
SfaNI GCATC 3 cut(s) 68, 347, 964
SgrAI CRCCGGYG 1 cut(s) 824
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 7 cut(s) 209, 278, 422, 503, 549, 845, 916
SsiI CCGC 3 cut(s) 76, 459, 813
StyD4I CCNGG 4 cut(s) 144, 363, 642, 979
StyI CCWWGG 3 cut(s) 45, 54, 565
TaaI ACNGT 4 cut(s) 218, 516, 539, 728
TaiI ACGT 2 cut(s) 74, 935
TaqI TCGA 2 cut(s) 204, 474
TasI AATT 7 cut(s) 209, 278, 422, 503, 549, 845, 916
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 3 cut(s) 317, 615, 958
Tru1I TTAA 3 cut(s) 552, 582, 908
Tru9I TTAA 3 cut(s) 552, 582, 908
TscAI CASTG 7 cut(s) 122, 223, 319, 468, 519, 628, 733
TseI GCWGC 8 cut(s) 101, 307, 332, 335, 467, 561, 686, 689
TspDTI ATGAA 4 cut(s) 137, 683, 909, 968
TspGWI ACGGA 2 cut(s) 624, 760
TspRI CASTG 7 cut(s) 122, 223, 319, 468, 519, 628, 733
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 4 cut(s) 209, 278, 422, 916
XceI RCATGY 1 cut(s) 663
XcmI CCANNNNNNNNNTGG 2 cut(s) 494, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.