Rw5G041490

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000341
Physical Location & Seq
Forward (+)
73041269 .. 73042400
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G041490.1

Sequence Viewer

Length: 1026 bp
ATGGATTTCAAAAGAAACCATGTGATCAGTGCCATATTCATCATCTTAGGGAATTTGGCTTCTCAAGCCACATCCGATGCATTGTACGAATCTTCCATTGCCGAAAAACATGAGCAATGGATGGCAAAGCATGGACGTGTTTACCCCAATGCGGCAGAGAAGGAAAGGCGTTTCGGCATCTTCAGGAAGAATGTCGAATACGTGGAGAAGTTCAACAATGAAGGGAATAAGACTTACAAGTTGAACCTCAACAGCTTTGCGGATATGACCAACGAAGAATTCCGGAGACATCATACCGGATACAGAACCCCCCCCGGCTTAAGTTCAACGTCATCCAATTTTAGCTACCAAAGCTTGGCTGCTACTGATATTCCAACTACCGTGGACTGGAGGGAACAACAAGCTGTCACCCCCATAAAGTATCAAAGTCAATGCGGTGCTTGCTGGGCATTTGCAGTAGCAGCAACTGTGGAAGGGCTAACCAAAATCAAAACAGGCCAATTGATCTCACTCTCCGAGCAGCAACTTGTGGACTGCAATCATCAGAACACTGGGTGCAAGGGTGGTTACTTAGAATACGCCTATTCCTACGTAGTACAAAACGGAGGACTCGCTCGAGAAGAAACTTACCCGTACCAGGCAACAGACATGGGAACATGCGATACTAACAAGGAAAGTGAGCATGCAGCACGGATCACTGGCTATGAACGCGTCCCTTCCAGAAGTGAAAACGATCTACAGAAGGCTGTGGCCATGCAACCAGTATCGGTTACCATTTCTGCTTATGGACAAGATTTTCAGATGTACGGCAGTGGGGTGTTCTCCGGCTATTGTGGGACGGAGCTCGACCACGCCGTCACGGTCATTGGCTACGGGACGATTGAAGATGGAACCCCCTATTGGTTAATAAAGAACTCATGGGGGCAGAGTTGGGGTGAAAGTGGGTATATGAGAATTCGTAGAAACATTAATGCCCCAGAGGGTATGTGCGGCCTTGCTATCAATCCCCTCTATCCGACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

341

Amino Acids

38.07

Weight (kDa)

5.94

Isoelectric Point (pI)

37.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 1.3e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 124 - 339 5.6e-80 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 854
AccB7I CCANNNNNTGG 1 cut(s) 355
AccII CGCG 1 cut(s) 711
AccIII TCCGGA 1 cut(s) 282
AciI CCGC 4 cut(s) 152, 260, 435, 990
AclWI GGATC 1 cut(s) 701
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 3 cut(s) 52, 278, 954
AcuI CTGAAG 1 cut(s) 166
AdeI CACNNNGTG 1 cut(s) 555
AfaI GTAC 4 cut(s) 86, 597, 635, 806
AfiI CCNNNNNNNGG 5 cut(s) 151, 355, 387, 637, 900
AflII CTTAAG 1 cut(s) 319
AflIII ACRYGT 2 cut(s) 136, 709
AgsI TTSAA 5 cut(s) 10, 214, 244, 327, 884
AjiI CACGTC 1 cut(s) 137
AjnI CCWGG 1 cut(s) 636
AjuI GAANNNNNNNTTGG 4 cut(s) 263, 295, 883, 915
AluBI AGCT 5 cut(s) 255, 345, 354, 404, 844
AluI AGCT 5 cut(s) 255, 345, 354, 404, 844
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 1 cut(s) 701
AlwNI CAGNNNCTG 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 615
Aor13HI TCCGGA 1 cut(s) 282
AoxI GGCC 3 cut(s) 496, 750, 991
ApeKI GCWGC 4 cut(s) 359, 461, 520, 686
ApoI RAATTY 3 cut(s) 52, 278, 954
AseI ATTAAT 1 cut(s) 969
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 400, 947
AvaI CYCGRG 1 cut(s) 615
BalI TGGCCA 1 cut(s) 752
BanII GRGCYC 1 cut(s) 846
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 4 cut(s) 346, 473, 532, 698
BccI CCATC 2 cut(s) 115, 881
BceAI ACGGC 2 cut(s) 823, 839
BciT130I CCWGG 1 cut(s) 638
BciVI GTATCC 1 cut(s) 293
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 280
BfmI CTRYAG 1 cut(s) 737
BfrI CTTAAG 1 cut(s) 319
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 6 cut(s) 153, 360, 462, 521, 687, 991
BlsI GCNGC 6 cut(s) 154, 361, 463, 522, 688, 992
Bme1390I CCNGG 2 cut(s) 315, 638
BmeT110I CYCGRG 1 cut(s) 615
BmgBI CACGTC 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 892
BmrFI CCNGG 2 cut(s) 315, 638
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 2 cut(s) 67, 186
BmuI ACTGGG 1 cut(s) 561
BpmI CTGGAG 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 48
BpuMI CCSGG 1 cut(s) 315
BsaAI YACGTR 2 cut(s) 202, 592
BsaJI CCNNGG 2 cut(s) 313, 381
BsaWI WCCGGW 2 cut(s) 282, 296
Bsc4I CCNNNNNNNGG 5 cut(s) 151, 355, 387, 637, 900
Bse1I ACTGG 4 cut(s) 392, 556, 703, 761
Bse3DI GCAATG 2 cut(s) 96, 122
BseAI TCCGGA 1 cut(s) 282
BseBI CCWGG 1 cut(s) 638
BseDI CCNNGG 2 cut(s) 313, 381
BseGI GGATG 3 cut(s) 71, 126, 332
BseLI CCNNNNNNNGG 5 cut(s) 151, 355, 387, 637, 900
BseMI GCAATG 2 cut(s) 96, 122
BseNI ACTGG 4 cut(s) 392, 556, 703, 761
BseXI GCAGC 4 cut(s) 346, 473, 532, 698
BseYI CCCAGC 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 3 cut(s) 498, 752, 993
BsiHKAI GWGCWC 1 cut(s) 846
BsiHKCI CYCGRG 1 cut(s) 615
BsiSI CCGG 4 cut(s) 283, 297, 315, 825
BslFI GGGAC 3 cut(s) 698, 850, 889
BslI CCNNNNNNNGG 5 cut(s) 151, 355, 387, 637, 900
BsmAI GTCTC 1 cut(s) 280
BsmFI GGGAC 3 cut(s) 698, 850, 889
BsnI GGCC 3 cut(s) 498, 752, 993
BsoBI CYCGRG 1 cut(s) 615
Bsp1286I GDGCHC 1 cut(s) 846
Bsp13I TCCGGA 1 cut(s) 282
Bsp143I GATC 4 cut(s) 24, 504, 693, 733
BspACI CCGC 4 cut(s) 152, 260, 435, 990
BspANI GGCC 3 cut(s) 498, 752, 993
BspEI TCCGGA 1 cut(s) 282
BspFNI CGCG 1 cut(s) 711
BspLI GGNNCC 1 cut(s) 892
BspPI GGATC 1 cut(s) 701
BspTI CTTAAG 1 cut(s) 319
BsrDI GCAATG 2 cut(s) 96, 122
BsrI ACTGG 4 cut(s) 392, 556, 703, 761
BssECI CCNNGG 2 cut(s) 313, 381
BssMI GATC 4 cut(s) 24, 504, 693, 733
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 3 cut(s) 382, 469, 862
BstAFI CTTAAG 1 cut(s) 319
BstBAI YACGTR 2 cut(s) 202, 592
BstC8I GCNNGC 2 cut(s) 442, 684
BstDEI CTNAG 2 cut(s) 46, 571
BstDSI CCRYGG 1 cut(s) 381
BstEII GGTNACC 1 cut(s) 769
BstF5I GGATG 3 cut(s) 71, 126, 332
BstFNI CGCG 1 cut(s) 711
BstKTI GATC 4 cut(s) 27, 507, 696, 736
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 4 cut(s) 24, 504, 693, 733
BstMWI GCNNNNNNNGC 6 cut(s) 65, 351, 441, 446, 461, 708
BstNI CCWGG 1 cut(s) 638
BstNSI RCATGY 2 cut(s) 660, 686
BstPI GGTNACC 1 cut(s) 769
BstSCI CCNGG 2 cut(s) 313, 636
BstSFI CTRYAG 1 cut(s) 737
BstSNI TACGTA 1 cut(s) 592
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 4 cut(s) 346, 473, 532, 698
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 3 cut(s) 498, 752, 993
BtgI CCRYGG 1 cut(s) 381
BtrI CACGTC 1 cut(s) 137
BtsCI GGATG 3 cut(s) 71, 126, 332
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 4 cut(s) 34, 549, 696, 817
Cac8I GCNNGC 2 cut(s) 442, 684
CaiI CAGNNNCTG 1 cut(s) 467
CseI GACGC 1 cut(s) 700
Csp6I GTAC 4 cut(s) 85, 596, 634, 805
CspCI CAANNNNNGTGG 2 cut(s) 363, 398
CviAII CATG 8 cut(s) 20, 110, 131, 649, 657, 683, 754, 918
CviQI GTAC 4 cut(s) 85, 596, 634, 805
DdeI CTNAG 2 cut(s) 46, 571
DpnI GATC 4 cut(s) 26, 506, 695, 735
DpnII GATC 4 cut(s) 24, 504, 693, 733
DraIII CACNNNGTG 1 cut(s) 555
DrdI GACNNNNNNGTC 1 cut(s) 854
DseDI GACNNNNNNGTC 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 750
Ecl136II GAGCTC 1 cut(s) 844
Eco105I TACGTA 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 846
Eco53kI GAGCTC 1 cut(s) 844
Eco57I CTGAAG 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 615
Eco91I GGTNACC 1 cut(s) 769
EcoICRI GAGCTC 1 cut(s) 844
EcoO65I GGTNACC 1 cut(s) 769
EcoRI GAATTC 2 cut(s) 278, 954
EcoRII CCWGG 1 cut(s) 636
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 846
FaeI CATG 8 cut(s) 23, 113, 134, 652, 660, 686, 757, 921
FaqI GGGAC 3 cut(s) 698, 850, 889
FatI CATG 8 cut(s) 19, 109, 130, 648, 656, 682, 753, 917
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 6 cut(s) 153, 360, 462, 521, 687, 991
FokI GGATG 3 cut(s) 58, 133, 319
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 6 cut(s) 153, 360, 462, 521, 687, 991
GluI GCNGC 6 cut(s) 153, 360, 462, 521, 687, 991
GsaI CCCAGC 1 cut(s) 448
GsuI CTGGAG 1 cut(s) 409
HaeIII GGCC 3 cut(s) 498, 752, 993
HapII CCGG 4 cut(s) 283, 297, 315, 825
HgaI GACGC 1 cut(s) 700
Hin1II CATG 8 cut(s) 23, 113, 134, 652, 660, 686, 757, 921
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 2 cut(s) 89, 609
HpaII CCGG 4 cut(s) 283, 297, 315, 825
HphI GGTGA 2 cut(s) 400, 947
Hpy166II GTNNAC 3 cut(s) 142, 385, 532
Hpy188I TCNGA 5 cut(s) 76, 517, 546, 801, 1017
Hpy188III TCNNGA 4 cut(s) 184, 283, 617, 720
Hpy8I GTNNAC 3 cut(s) 142, 385, 532
HpyAV CCTTC 5 cut(s) 154, 215, 467, 726, 736
HpyCH4III ACNGT 3 cut(s) 382, 469, 862
HpyCH4IV ACGT 4 cut(s) 136, 201, 329, 591
HpyCH4V TGCA 7 cut(s) 80, 455, 537, 558, 686, 757, 1022
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 351, 441, 446, 461, 708
HpyF3I CTNAG 2 cut(s) 46, 571
HpySE526I ACGT 4 cut(s) 136, 201, 329, 591
Hsp92II CATG 8 cut(s) 23, 113, 134, 652, 660, 686, 757, 921
Kpn2I TCCGGA 1 cut(s) 282
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 4 cut(s) 24, 504, 693, 733
LmnI GCTCC 1 cut(s) 841
Lsp1109I GCAGC 4 cut(s) 346, 473, 532, 698
LweI GCATC 2 cut(s) 67, 186
MaeII ACGT 4 cut(s) 136, 201, 329, 591
MaeIII GTNAC 4 cut(s) 406, 566, 769, 856
MalI GATC 4 cut(s) 26, 506, 695, 735
MboI GATC 4 cut(s) 24, 504, 693, 733
MboII GAAGA 6 cut(s) 84, 172, 199, 287, 632, 896
MfeI CAATTG 1 cut(s) 500
MhlI GDGCHC 1 cut(s) 846
MlsI TGGCCA 1 cut(s) 752
MluCI AATT 5 cut(s) 52, 278, 337, 500, 954
MluI ACGCGT 1 cut(s) 709
MluNI TGGCCA 1 cut(s) 752
MlyI GAGTC 1 cut(s) 603
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 5 cut(s) 257, 384, 599, 973, 1019
Mox20I TGGCCA 1 cut(s) 752
Mph1103I ATGCAT 1 cut(s) 82
MroI TCCGGA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 752
MseI TTAA 3 cut(s) 320, 905, 969
MslI CAYNNNNRTG 1 cut(s) 135
Msp20I TGGCCA 1 cut(s) 752
MspCI CTTAAG 1 cut(s) 319
MspI CCGG 4 cut(s) 283, 297, 315, 825
MspR9I CCNGG 2 cut(s) 315, 638
MunI CAATTG 1 cut(s) 500
MvaI CCWGG 1 cut(s) 638
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 6 cut(s) 65, 351, 441, 446, 461, 708
NciI CCSGG 1 cut(s) 315
NdeII GATC 4 cut(s) 24, 504, 693, 733
NlaIII CATG 8 cut(s) 23, 113, 134, 652, 660, 686, 757, 921
NlaIV GGNNCC 1 cut(s) 892
NmuCI GTSAC 2 cut(s) 406, 856
NsiI ATGCAT 1 cut(s) 82
NspI RCATGY 2 cut(s) 660, 686
PaeI GCATGC 1 cut(s) 686
PaeR7I CTCGAG 1 cut(s) 615
PcsI WCGNNNNNNNCGW 1 cut(s) 852
PfeI GAWTC 1 cut(s) 89
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 6 cut(s) 154, 361, 463, 522, 688, 992
PleI GAGTC 1 cut(s) 603
PpsI GAGTC 1 cut(s) 603
Ppu21I YACGTR 2 cut(s) 202, 592
PshBI ATTAAT 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 846
Psp6I CCWGG 1 cut(s) 636
PspEI GGTNACC 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 444
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 1 cut(s) 892
PsrI GAACNNNNNNTAC 2 cut(s) 646, 678
PstNI CAGNNNCTG 1 cut(s) 467
RsaI GTAC 4 cut(s) 86, 597, 635, 806
RsaNI GTAC 4 cut(s) 85, 596, 634, 805
RseI CAYNNNNRTG 1 cut(s) 135
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 3 cut(s) 320, 905, 969
SatI GCNGC 6 cut(s) 153, 360, 462, 521, 687, 991
Sau3AI GATC 4 cut(s) 24, 504, 693, 733
SchI GAGTC 1 cut(s) 603
ScrFI CCNGG 2 cut(s) 315, 638
SduI GDGCHC 1 cut(s) 846
SfaNI GCATC 2 cut(s) 67, 186
SfcI CTRYAG 1 cut(s) 737
Sfr274I CTCGAG 1 cut(s) 615
SlaI CTCGAG 1 cut(s) 615
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 3 cut(s) 63, 319, 615
SmoI CTYRAG 3 cut(s) 63, 319, 615
SnaBI TACGTA 1 cut(s) 592
SphI GCATGC 1 cut(s) 686
Sse9I AATT 5 cut(s) 52, 278, 337, 500, 954
SsiI CCGC 4 cut(s) 152, 260, 435, 990
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 2 cut(s) 313, 636
TaaI ACNGT 3 cut(s) 382, 469, 862
TaiI ACGT 4 cut(s) 139, 204, 332, 594
TaqI TCGA 3 cut(s) 195, 616, 846
TasI AATT 5 cut(s) 52, 278, 337, 500, 954
TatI WGTACW 1 cut(s) 595
TauI GCSGC 2 cut(s) 155, 993
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 3 cut(s) 320, 905, 969
Tru9I TTAA 3 cut(s) 320, 905, 969
TscAI CASTG 4 cut(s) 34, 556, 703, 817
TseFI GTSAC 2 cut(s) 406, 856
TseI GCWGC 4 cut(s) 359, 461, 520, 686
Tsp45I GTSAC 2 cut(s) 406, 856
TspDTI ATGAA 3 cut(s) 28, 234, 720
TspGWI ACGGA 3 cut(s) 618, 706, 854
TspRI CASTG 4 cut(s) 34, 556, 703, 817
Van91I CCANNNNNTGG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 319
VspI ATTAAT 1 cut(s) 969
XapI RAATTY 3 cut(s) 52, 278, 954
XceI RCATGY 2 cut(s) 660, 686
XhoI CTCGAG 1 cut(s) 615
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.