Rh5BG076500

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000341
Physical Location & Seq
Reverse (-)
6426792 .. 6429506
2715 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG076500.1

Sequence Viewer

Length: 1041 bp
ATGGCTTTTGAACGAGTTAACCTTGTGATTGTTACTACACTGGTCACCATGGGAGTTTTGGCTTCGCAAGCCGCATCCCTTTCACTGTACGAAGCTTCCATTGCTGAGAAACATGAGCAATGGATGGAGAAGTACGGACGCGTTTACCCCAACAATGCAGAGAAGCAAAGGCGTTTCGCTATCTTCATGAAGAATGTCGAATTTGTGGAGAAATCCAACAGTGAAGGGAACAAGACTTACAAGTTGAGCATCAACGAATTTTCTGATATGACTAATGAAGAATTCATGAGACATCATACTGGATACAAAATCCCCACCAGCTCGAGTTCAACCTCTTCCGAACATAAATCTTTTCGGTACCAAAGCTTGGCTGCAACTGAGGTTCCAGCTAGCATGGACTGGAGGGAGCAAGGAGCTGTTACCCGCATAAGAAATCAAGGTCAATATTGTGGTGCTTGTTGGGCATTTACCGTAGTGGCAGCAGTAGAAGGGCTAATCCAAATCAAAACTGGCCAATTGATCCCGCTGTCCGAGCAACAACTTGTCGACTGTGATCACACCAACAATGGCTGCAACGGTGGCAGTGAAGTGAATGCATTTAAATACATAATACAAAATGGAGGAATCGCTAGTGAAGAAAATTACCAATACCAGAGCACAGACATGGGAACATGTGACACTAACAAGGAAAGTGACCATGCTGCCCAGATCACTGGTTATGAAATAGTCCCTTCCAGAAGTGAAGATGATCTACTCAAGGCTGTGTCCAAGCAACCAGTCTCGGTTAGCATTGATGCTTCTGGTGAAGCGTTTCAGCATTACAGGAGTGGGGTGTTCTCCGGTAATTGTGGGACGAACCTCCATCATGCTGTTGCAGCCATTGGGTACGGAACAACTGAAGGTGAAATCGACTATTGGTTATTGAAGAATTCGTGGGGTGAGACTTGGGGTGAAAATGGCTATATGAAAATTCTTAGAAACGTTGATGCCCCGGAAGGTATGTGTGGCCTTGCTATCAGAGCTTCCTATCCGACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

346

Amino Acids

38.3

Weight (kDa)

5.27

Isoelectric Point (pI)

31.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 38 - 95 2.2e-21 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 128 - 345 3.5e-77 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 367
AccI GTMKAC 1 cut(s) 546
AccII CGCG 1 cut(s) 141
AciI CCGC 3 cut(s) 72, 424, 524
AclI AACGTT 1 cut(s) 981
AclWI GGATC 1 cut(s) 514
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 5 cut(s) 200, 257, 281, 928, 969
AcuI CTGAAG 1 cut(s) 918
AfaI GTAC 4 cut(s) 89, 134, 359, 887
AfiI CCNNNNNNNGG 1 cut(s) 367
AflIII ACRYGT 2 cut(s) 139, 671
AgsI TTSAA 3 cut(s) 11, 330, 925
AluBI AGCT 6 cut(s) 95, 321, 366, 389, 416, 1022
AluI AGCT 6 cut(s) 95, 321, 366, 389, 416, 1022
Alw21I GWGCWC 1 cut(s) 659
Alw26I GTCTC 3 cut(s) 283, 784, 935
AlwI GGATC 1 cut(s) 514
Ama87I CYCGRG 1 cut(s) 322
AoxI GGCC 2 cut(s) 511, 1006
ApeKI GCWGC 5 cut(s) 371, 479, 570, 701, 875
ApoI RAATTY 5 cut(s) 200, 257, 281, 928, 969
Asp700I GAANNNNTTC 1 cut(s) 810
Asp718I GGTACC 1 cut(s) 357
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 5 cut(s) 37, 815, 914, 950, 962
AsuNHI GCTAGC 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 322
BalI TGGCCA 1 cut(s) 513
BanI GGYRCC 1 cut(s) 357
BarI GAAGNNNNNNTAC 2 cut(s) 735, 767
Bbv12I GWGCWC 1 cut(s) 659
BbvI GCAGC 5 cut(s) 358, 491, 557, 688, 887
BccI CCATC 2 cut(s) 118, 870
BciVI GTATCC 1 cut(s) 296
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 992
BcoDI GTCTC 3 cut(s) 283, 784, 935
BfaI CTAG 2 cut(s) 390, 630
BfuI GTATCC 1 cut(s) 296
BisI GCNGC 6 cut(s) 72, 372, 480, 571, 702, 876
BlsI GCNGC 6 cut(s) 73, 373, 481, 572, 703, 877
Bme1390I CCNGG 1 cut(s) 992
BmeT110I CYCGRG 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 359, 384
BmrFI CCNGG 1 cut(s) 992
BmsI GCATC 4 cut(s) 83, 258, 784, 976
BmtI GCTAGC 1 cut(s) 393
BpmI CTGGAG 1 cut(s) 421
BpuEI CTTGAG 1 cut(s) 740
BpuMI CCSGG 1 cut(s) 992
BsaJI CCNNGG 2 cut(s) 48, 990
BsaWI WCCGGW 1 cut(s) 839
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 6 cut(s) 45, 304, 404, 514, 718, 776
Bse3DI GCAATG 2 cut(s) 99, 125
BseDI CCNNGG 2 cut(s) 48, 990
BseGI GGATG 2 cut(s) 74, 129
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 2 cut(s) 99, 125
BseMII CTCAG 2 cut(s) 96, 369
BseNI ACTGG 6 cut(s) 45, 304, 404, 514, 718, 776
BseXI GCAGC 5 cut(s) 358, 491, 557, 688, 887
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 2 cut(s) 513, 1008
BshNI GGYRCC 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 659
BsiHKCI CYCGRG 1 cut(s) 322
BsiSI CCGG 2 cut(s) 840, 992
BslFI GGGAC 2 cut(s) 713, 865
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 3 cut(s) 283, 784, 935
BsmFI GGGAC 2 cut(s) 713, 865
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 2 cut(s) 513, 1008
BsoBI CYCGRG 1 cut(s) 322
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 4 cut(s) 519, 553, 708, 748
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 3 cut(s) 72, 424, 524
BspANI GGCC 2 cut(s) 513, 1008
BspCNI CTCAG 2 cut(s) 97, 370
BspFNI CGCG 1 cut(s) 141
BspHI TCATGA 2 cut(s) 186, 285
BspLI GGNNCC 2 cut(s) 359, 384
BspOI GCTAGC 1 cut(s) 393
BspPI GGATC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 357
BsrDI GCAATG 2 cut(s) 99, 125
BsrI ACTGG 6 cut(s) 45, 304, 404, 514, 718, 776
BssECI CCNNGG 2 cut(s) 48, 990
BssMI GATC 4 cut(s) 519, 553, 708, 748
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 5 cut(s) 87, 221, 472, 551, 578
Bst6I CTCTTC 1 cut(s) 340
BstC8I GCNNGC 2 cut(s) 69, 391
BstDEI CTNAG 3 cut(s) 105, 378, 974
BstDSI CCRYGG 1 cut(s) 48
BstEII GGTNACC 1 cut(s) 43
BstF5I GGATG 2 cut(s) 74, 129
BstFNI CGCG 1 cut(s) 141
BstKTI GATC 4 cut(s) 522, 556, 711, 751
BstMAI GTCTC 3 cut(s) 283, 784, 935
BstMBI GATC 4 cut(s) 519, 553, 708, 748
BstMWI GCNNNNNNNGC 7 cut(s) 68, 101, 461, 532, 579, 875, 1019
BstNSI RCATGY 1 cut(s) 675
BstPI GGTNACC 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 990
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 5 cut(s) 358, 491, 557, 688, 887
BstXI CCANNNNNNTGG 1 cut(s) 713
BsuI GTATCC 1 cut(s) 296
BsuRI GGCC 2 cut(s) 513, 1008
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 2 cut(s) 74, 129
BtsI GCAGTG 1 cut(s) 589
BtsIMutI CAGTG 5 cut(s) 38, 83, 226, 589, 711
Cac8I GCNNGC 2 cut(s) 69, 391
CciI TCATGA 2 cut(s) 186, 285
CseI GACGC 1 cut(s) 147
Csp6I GTAC 4 cut(s) 88, 133, 358, 886
CviAII CATG 9 cut(s) 49, 113, 187, 286, 394, 664, 672, 698, 866
CviQI GTAC 4 cut(s) 88, 133, 358, 886
DdeI CTNAG 3 cut(s) 105, 378, 974
DpnI GATC 4 cut(s) 521, 555, 710, 750
DpnII GATC 4 cut(s) 519, 553, 708, 748
DraI TTTAAA 1 cut(s) 601
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 48
Eco57I CTGAAG 1 cut(s) 918
Eco88I CYCGRG 1 cut(s) 322
Eco91I GGTNACC 1 cut(s) 43
EcoO65I GGTNACC 1 cut(s) 43
EcoRI GAATTC 2 cut(s) 281, 928
EcoT14I CCWWGG 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 598
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 9 cut(s) 52, 116, 190, 289, 397, 667, 675, 701, 869
FaqI GGGAC 2 cut(s) 713, 865
FatI CATG 9 cut(s) 48, 112, 186, 285, 393, 663, 671, 697, 865
FauI CCCGC 2 cut(s) 431, 531
FbaI TGATCA 1 cut(s) 553
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 6 cut(s) 72, 372, 480, 571, 702, 876
FokI GGATG 2 cut(s) 61, 136
Fsp4HI GCNGC 6 cut(s) 72, 372, 480, 571, 702, 876
FspBI CTAG 2 cut(s) 390, 630
GluI GCNGC 6 cut(s) 72, 372, 480, 571, 702, 876
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 2 cut(s) 513, 1008
HapII CCGG 2 cut(s) 840, 992
HgaI GACGC 1 cut(s) 147
Hin1II CATG 9 cut(s) 52, 116, 190, 289, 397, 667, 675, 701, 869
HincII GTYRAC 2 cut(s) 19, 547
HindII GTYRAC 2 cut(s) 19, 547
HindIII AAGCTT 2 cut(s) 93, 364
HinfI GANTC 1 cut(s) 624
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 2 cut(s) 840, 992
HphI GGTGA 5 cut(s) 37, 815, 914, 950, 962
Hpy166II GTNNAC 3 cut(s) 19, 145, 547
Hpy188I TCNGA 5 cut(s) 265, 340, 532, 1019, 1032
Hpy188III TCNNGA 3 cut(s) 187, 286, 735
Hpy8I GTNNAC 3 cut(s) 19, 145, 547
HpyAV CCTTC 5 cut(s) 218, 482, 741, 893, 989
HpyCH4III ACNGT 5 cut(s) 87, 221, 472, 551, 578
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 6 cut(s) 158, 374, 573, 596, 875, 1037
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 101, 461, 532, 579, 875, 1019
HpyF3I CTNAG 3 cut(s) 105, 378, 974
HpySE526I ACGT 1 cut(s) 981
Hsp92II CATG 9 cut(s) 52, 116, 190, 289, 397, 667, 675, 701, 869
KpnI GGTACC 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 553
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 4 cut(s) 519, 553, 708, 748
LmnI GCTCC 2 cut(s) 406, 413
Lsp1109I GCAGC 5 cut(s) 358, 491, 557, 688, 887
LweI GCATC 4 cut(s) 83, 258, 784, 976
MaeI CTAG 2 cut(s) 390, 630
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 5 cut(s) 31, 43, 418, 674, 692
MalI GATC 4 cut(s) 521, 555, 710, 750
MboI GATC 4 cut(s) 519, 553, 708, 748
MboII GAAGA 7 cut(s) 175, 202, 290, 327, 647, 755, 937
MfeI CAATTG 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 8 cut(s) 200, 257, 281, 515, 640, 844, 928, 969
MluI ACGCGT 1 cut(s) 139
MluNI TGGCCA 1 cut(s) 513
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 5 cut(s) 343, 373, 396, 614, 869
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 598
MroXI GAANNNNTTC 1 cut(s) 810
MscI TGGCCA 1 cut(s) 513
MseI TTAA 2 cut(s) 18, 600
MslI CAYNNNNRTG 1 cut(s) 662
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 526
MspI CCGG 2 cut(s) 840, 992
MspR9I CCNGG 1 cut(s) 992
MunI CAATTG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 598
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 7 cut(s) 68, 101, 461, 532, 579, 875, 1019
NciI CCSGG 1 cut(s) 992
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 519, 553, 708, 748
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 9 cut(s) 52, 116, 190, 289, 397, 667, 675, 701, 869
NlaIV GGNNCC 2 cut(s) 359, 384
NmuCI GTSAC 3 cut(s) 43, 674, 692
NsiI ATGCAT 1 cut(s) 598
NspI RCATGY 1 cut(s) 675
PaeR7I CTCGAG 1 cut(s) 322
PagI TCATGA 2 cut(s) 186, 285
PciI ACATGT 1 cut(s) 671
PctI GAATGC 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 1 cut(s) 624
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 6 cut(s) 73, 373, 481, 572, 703, 877
PscI ACATGT 1 cut(s) 671
Psp1406I AACGTT 1 cut(s) 981
PspEI GGTNACC 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 359, 384
PspXI VCTCGAGB 1 cut(s) 322
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
RsaI GTAC 4 cut(s) 89, 134, 359, 887
RsaNI GTAC 4 cut(s) 88, 133, 358, 886
RseI CAYNNNNRTG 1 cut(s) 662
SalI GTCGAC 1 cut(s) 545
SaqAI TTAA 2 cut(s) 18, 600
SatI GCNGC 6 cut(s) 72, 372, 480, 571, 702, 876
Sau3AI GATC 4 cut(s) 519, 553, 708, 748
ScrFI CCNGG 1 cut(s) 992
SduI GDGCHC 1 cut(s) 659
SfaNI GCATC 4 cut(s) 83, 258, 784, 976
Sfr274I CTCGAG 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 322
SmiI ATTTAAAT 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 2 cut(s) 322, 755
SmoI CTYRAG 2 cut(s) 322, 755
Sse9I AATT 8 cut(s) 200, 257, 281, 515, 640, 844, 928, 969
SsiI CCGC 3 cut(s) 72, 424, 524
SspI AATATT 1 cut(s) 446
SspMI CTAG 2 cut(s) 390, 630
StyD4I CCNGG 1 cut(s) 990
StyI CCWWGG 1 cut(s) 48
SwaI ATTTAAAT 1 cut(s) 601
TaaI ACNGT 5 cut(s) 87, 221, 472, 551, 578
TaiI ACGT 1 cut(s) 984
TaqI TCGA 4 cut(s) 198, 323, 546, 909
TasI AATT 8 cut(s) 200, 257, 281, 515, 640, 844, 928, 969
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 1 cut(s) 624
Tru1I TTAA 2 cut(s) 18, 600
Tru9I TTAA 2 cut(s) 18, 600
TscAI CASTG 5 cut(s) 45, 90, 226, 589, 718
TseFI GTSAC 3 cut(s) 43, 674, 692
TseI GCWGC 5 cut(s) 371, 479, 570, 701, 875
Tsp45I GTSAC 3 cut(s) 43, 674, 692
TspDTI ATGAA 6 cut(s) 175, 203, 274, 291, 735, 980
TspGWI ACGGA 2 cut(s) 150, 903
TspRI CASTG 5 cut(s) 45, 90, 226, 589, 718
Van91I CCANNNNNTGG 1 cut(s) 367
XapI RAATTY 5 cut(s) 200, 257, 281, 928, 969
XceI RCATGY 1 cut(s) 675
XcmI CCANNNNNNNNNTGG 2 cut(s) 55, 506
XhoI CTCGAG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 546
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 2 cut(s) 390, 630
Zsp2I ATGCAT 1 cut(s) 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.