Rw5G007480

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000341
Physical Location & Seq
Forward (+)
7911080 .. 7912040
961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G007480.1

Sequence Viewer

Length: 549 bp
ATGGCTTTGGAAGGAAACCTTGTGCTTGTCACACAGCTTGCTGTTGCCATATTATCCCTCCGTTTGTTGGGTACTTTGGCAATATCCCAGGCCACATCCCGCTCATCTTATGGAGTCAATTCCACCAACATTGCCCAAACCTTTGAGCAATGGATGGCAAAGTACGGCCGCATTTACCCTAACGGCCAGGAGAAGGGAAAACGTTCTGCCATATTCCGGACCAATTTGGAGTATGTTGAGAGCTTCAACAAACAAGAGAGTAAGACTTACAAGTTAGGCCTGAATCAATTTTCTGATATGACCAATGCAGAATTTTTCCGACATCATACCGGATACAAAAGAATGCCCACCTCCTCAACCTCTTTTAGGTACCAAAACATGTCCGATGATGATGTCCCCAATAGCATTGATTGGAGGAAACAAGGAGCTGTCACCCCTGTAAAGTTGTTGTTGGGCTTTCTCAGCGGTGGCAGCTGTGGAAGGGATTACCAAAATAAGAACTGGCCAAATGATCCCGCTGTCCGAGCAACAACTTGTGGACTGCAATAG

Protein Analysis

182

Amino Acids

20.42

Weight (kDa)

9.51

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 48 - 105 1e-21 Cathepsin propeptide inhibitor domain (I29)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 369
AccBSI CCGCTC 1 cut(s) 102
AccIII TCCGGA 1 cut(s) 216
AciI CCGC 4 cut(s) 100, 169, 465, 516
AclI AACGTT 1 cut(s) 202
AclWI GGATC 1 cut(s) 506
AcoI YGGCCR 3 cut(s) 166, 184, 503
AcsI RAATTY 1 cut(s) 311
AfaI GTAC 3 cut(s) 73, 164, 371
AfiI CCNNNNNNNGG 4 cut(s) 67, 193, 216, 366
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 2 cut(s) 87, 186
AluBI AGCT 4 cut(s) 37, 243, 428, 474
AluI AGCT 4 cut(s) 37, 243, 428, 474
AlwI GGATC 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 216
AoxI GGCC 5 cut(s) 90, 166, 184, 277, 503
ApeKI GCWGC 1 cut(s) 471
ApoI RAATTY 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 202
Asp718I GGTACC 1 cut(s) 369
AspS9I GGNCC 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 424
AvaII GGWCC 1 cut(s) 219
BalI TGGCCA 1 cut(s) 505
BanI GGYRCC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 483
BccI CCATC 1 cut(s) 148
BceAI ACGGC 2 cut(s) 181, 199
BciT130I CCWGG 2 cut(s) 89, 188
BciVI GTATCC 1 cut(s) 326
BfuI GTATCC 1 cut(s) 326
BisI GCNGC 2 cut(s) 169, 472
BlsI GCNGC 2 cut(s) 170, 473
Bme1390I CCNGG 2 cut(s) 89, 188
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 371
BmrFI CCNGG 2 cut(s) 89, 188
BsaJI CCNNGG 1 cut(s) 87
BsaWI WCCGGW 2 cut(s) 216, 329
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 193, 216, 366
Bse1I ACTGG 1 cut(s) 506
Bse3DI GCAATG 2 cut(s) 129, 155
BseAI TCCGGA 1 cut(s) 216
BseBI CCWGG 2 cut(s) 89, 188
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 2 cut(s) 95, 159
BseLI CCNNNNNNNGG 4 cut(s) 67, 193, 216, 366
BseMI GCAATG 2 cut(s) 129, 155
BseMII CTCAG 1 cut(s) 475
BseNI ACTGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 343
BseX3I CGGCCG 1 cut(s) 166
BseXI GCAGC 1 cut(s) 483
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 5 cut(s) 92, 168, 186, 279, 505
BshNI GGYRCC 1 cut(s) 369
BsiEI CGRYCG 1 cut(s) 169
BsiSI CCGG 2 cut(s) 217, 330
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 4 cut(s) 67, 193, 216, 366
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 5 cut(s) 92, 168, 186, 279, 505
Bsp13I TCCGGA 1 cut(s) 216
Bsp143I GATC 1 cut(s) 511
BspACI CCGC 4 cut(s) 100, 169, 465, 516
BspANI GGCC 5 cut(s) 92, 168, 186, 279, 505
BspCNI CTCAG 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 216
BspLI GGNNCC 1 cut(s) 371
BspPI GGATC 1 cut(s) 506
BspT107I GGYRCC 1 cut(s) 369
BsrBI CCGCTC 1 cut(s) 102
BsrDI GCAATG 2 cut(s) 129, 155
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 511
Bst2UI CCWGG 2 cut(s) 89, 188
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 1 cut(s) 461
BstENI CCTNNNNNAGG 1 cut(s) 364
BstF5I GGATG 2 cut(s) 95, 159
BstKTI GATC 1 cut(s) 514
BstMBI GATC 1 cut(s) 511
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 3 cut(s) 462, 471, 524
BstNI CCWGG 2 cut(s) 89, 188
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 87, 186
BstV1I GCAGC 1 cut(s) 483
BstZI CGGCCG 1 cut(s) 166
BsuI GTATCC 1 cut(s) 326
BsuRI GGCC 5 cut(s) 92, 168, 186, 279, 505
BtsCI GGATG 2 cut(s) 95, 159
Cac8I GCNNGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 219
Csp6I GTAC 3 cut(s) 72, 163, 370
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 1 cut(s) 379
CviQI GTAC 3 cut(s) 72, 163, 370
DdeI CTNAG 1 cut(s) 461
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
EaeI YGGCCR 3 cut(s) 166, 184, 503
EagI CGGCCG 1 cut(s) 166
EclXI CGGCCG 1 cut(s) 166
Eco147I AGGCCT 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 219
Eco52I CGGCCG 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 364
EcoRII CCWGG 2 cut(s) 87, 186
FaeI CATG 1 cut(s) 382
FaiI YATR 7 cut(s) 50, 111, 212, 234, 299, 327, 380
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 1 cut(s) 380
FatI CATG 1 cut(s) 378
FauI CCCGC 2 cut(s) 107, 523
Fnu4HI GCNGC 2 cut(s) 169, 472
FokI GGATG 2 cut(s) 82, 166
Fsp4HI GCNGC 2 cut(s) 169, 472
GluI GCNGC 2 cut(s) 169, 472
HaeIII GGCC 5 cut(s) 92, 168, 186, 279, 505
HapII CCGG 2 cut(s) 217, 330
Hin1II CATG 1 cut(s) 382
HinfI GANTC 2 cut(s) 114, 283
HpaII CCGG 2 cut(s) 217, 330
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 539
Hpy188I TCNGA 4 cut(s) 295, 320, 385, 524
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 1 cut(s) 539
HpyAV CCTTC 3 cut(s) 5, 187, 474
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 2 cut(s) 308, 544
HpyF10VI GCNNNNNNNGC 3 cut(s) 462, 471, 524
HpyF3I CTNAG 1 cut(s) 461
HpySE526I ACGT 1 cut(s) 202
Hsp92II CATG 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 216
KpnI GGTACC 1 cut(s) 373
Kzo9I GATC 1 cut(s) 511
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 9 cut(s) 74, 101, 173, 200, 230, 293, 343, 450, 487
Lsp1109I GCAGC 1 cut(s) 483
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 2 cut(s) 28, 430
MalI GATC 1 cut(s) 513
MbiI CCGCTC 1 cut(s) 102
MboI GATC 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 505
MluCI AATT 4 cut(s) 118, 223, 287, 311
MluNI TGGCCA 1 cut(s) 505
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 5 cut(s) 68, 361, 364, 370, 408
Mox20I TGGCCA 1 cut(s) 505
MroI TCCGGA 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 202
MscI TGGCCA 1 cut(s) 505
Msp20I TGGCCA 1 cut(s) 505
MspA1I CMGCKG 3 cut(s) 465, 474, 518
MspI CCGG 2 cut(s) 217, 330
MspR9I CCNGG 2 cut(s) 89, 188
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 2 cut(s) 89, 188
MwoI GCNNNNNNNGC 3 cut(s) 462, 471, 524
NdeII GATC 1 cut(s) 511
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 1 cut(s) 371
NmuCI GTSAC 2 cut(s) 28, 430
NspI RCATGY 1 cut(s) 382
PceI AGGCCT 1 cut(s) 279
PciI ACATGT 1 cut(s) 378
PctI GAATGC 1 cut(s) 348
PdmI GAANNNNTTC 1 cut(s) 202
PfeI GAWTC 1 cut(s) 283
PkrI GCNGC 2 cut(s) 170, 473
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
PscI ACATGT 1 cut(s) 378
Psp1406I AACGTT 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 87, 186
PspGI CCWGG 2 cut(s) 87, 186
PspN4I GGNNCC 1 cut(s) 371
PspPI GGNCC 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 474
RsaI GTAC 3 cut(s) 73, 164, 371
RsaNI GTAC 3 cut(s) 72, 163, 370
SatI GCNGC 2 cut(s) 169, 472
Sau3AI GATC 1 cut(s) 511
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 2 cut(s) 89, 188
SinI GGWCC 1 cut(s) 219
Sse9I AATT 4 cut(s) 118, 223, 287, 311
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 4 cut(s) 100, 169, 465, 516
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 87, 186
TaiI ACGT 1 cut(s) 205
TasI AATT 4 cut(s) 118, 223, 287, 311
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 283
TseFI GTSAC 2 cut(s) 28, 430
TseI GCWGC 1 cut(s) 471
Tsp45I GTSAC 2 cut(s) 28, 430
TspGWI ACGGA 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 219
XagI CCTNNNNNAGG 1 cut(s) 364
XapI RAATTY 1 cut(s) 311
XceI RCATGY 1 cut(s) 382
XmnI GAANNNNTTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.