MD05G1302800.v1.1

Cathepsin propeptide inhibitor domain (I29)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000341
Physical Location & Seq
Forward (+)
43559401 .. 43560535
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1302800.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGGCTTTTGAAAGAAACATTTTAATTGTTGCCATACTCATCACCTTGGGGACCTTGGCATCTCAAGCCACGTTTTCCATTGCTGCAAAACATGAGCAATGGATGGAAAAGTTTGGGCGCGTTTACCAGGACAGCGCAGAGAAGGAAAGGCGTTTCCACATATTCAAGAACAATGTGGAATTCGTGGAGAAATTCAACAGTGAAGGGAAGAAAAAATACAAGCTAAGCATCAATCAATTTTCTGATATGACCCATGAAGAGTTTCTCAGACATCATACTGGTTACAAGAAGCCCACTGAATCAACCTCATCCGCAGATGCATCTTTTACGTACGAAAGCCTAAGCCTGGCGGCTGTTCCCCCAAGCATTGACTGGAGGAAGCAAGATGCTGTTACTCCTGTAAAATATCAAGGGAACTGTGCTTGTTGCTGGGCATTTTCTGCAGTGGCAGCAGTCGAAGGGATAACCAAAATCAAAACTGGCCAATTGATCTCACTGTCTGAGCAACAACTTGTGAGCTGTGATACAAGTAACTTTGGCTGCCAAAATGGTGACATGGCTAAAGCCTTTGCATACATTCAACAGAACGGAGGAATTGCCAGTGAAAAAAATTACCCATACCAGGGTTCGGATGGAACATGTGATATGAACCGGGAAAATGAGGCTGCTGCCAAGATAACTAATTATGCAAGGGTAACTCCCAACAGTGAAACCGATTTGCTCAAGGCTGTATCCATGCAACCAGTCTCCGTTGCCATCGTTGCCTCACAAGCCTTTGAGCTCTATTCCGGCGGGCTGTTCACTGATGATAATTGTGGGACAGAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCACCCCCTATTGGTTAATCAAGAACTCATGGAGCACGTCGTGGGGTGAGAATGGCTACATGAAGATTCTTAGAAACGCCGATGCCCCAGAAGGTGTTTGTGGCCTCGCTATGGATGCTTCCTATCCAATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

336

Amino Acids

36.99

Weight (kDa)

5.37

Isoelectric Point (pI)

32.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 31 - 88 6.6e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 119 - 334 2.2e-79 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 872
AccII CGCG 1 cut(s) 120
AciI CCGC 3 cut(s) 312, 350, 792
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 2 cut(s) 179, 191
AcuI CTGAAG 1 cut(s) 885
AdeI CACNNNGTG 1 cut(s) 912
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 7 cut(s) 346, 622, 623, 628, 849, 882, 982
AflIII ACRYGT 1 cut(s) 638
AgsI TTSAA 5 cut(s) 11, 166, 196, 581, 829
AjiI CACGTC 1 cut(s) 909
AjnI CCWGG 3 cut(s) 126, 345, 621
AluBI AGCT 3 cut(s) 223, 519, 781
AluI AGCT 3 cut(s) 223, 519, 781
Alw21I GWGCWC 2 cut(s) 783, 908
Alw26I GTCTC 1 cut(s) 751
AoxI GGCC 2 cut(s) 481, 973
ApeKI GCWGC 5 cut(s) 83, 449, 540, 665, 668
ApoI RAATTY 2 cut(s) 179, 191
Asp700I GAANNNNTTC 1 cut(s) 261
AspLEI GCGC 2 cut(s) 120, 137
AspS9I GGNCC 1 cut(s) 51
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 3 cut(s) 34, 563, 929
AvaII GGWCC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 483
BanI GGYRCC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 783
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
Bbv12I GWGCWC 2 cut(s) 783, 908
BbvI GCAGC 5 cut(s) 70, 461, 527, 652, 655
BccI CCATC 5 cut(s) 97, 626, 764, 851, 863
BciT130I CCWGG 3 cut(s) 128, 347, 623
BciVI GTATCC 1 cut(s) 742
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 1 cut(s) 751
BfmI CTRYAG 1 cut(s) 441
BfuI GTATCC 1 cut(s) 742
BisI GCNGC 6 cut(s) 84, 351, 450, 541, 666, 669
BlpI GCTNAGC 1 cut(s) 224
BlsI GCNGC 6 cut(s) 85, 352, 451, 542, 667, 670
Bme1390I CCNGG 4 cut(s) 128, 347, 623, 653
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 909
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 2 cut(s) 52, 874
BmrFI CCNGG 4 cut(s) 128, 347, 623, 653
BmsI GCATC 7 cut(s) 68, 237, 307, 329, 376, 943, 976
BpmI CTGGAG 1 cut(s) 394
Bpu10I CCTNAGC 1 cut(s) 341
Bpu1102I GCTNAGC 1 cut(s) 224
BpuEI CTTGAG 2 cut(s) 48, 707
BpuMI CCSGG 1 cut(s) 653
BsaAI YACGTR 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 45, 54, 622
Bsc4I CCNNNNNNNGG 7 cut(s) 346, 622, 623, 628, 849, 882, 982
Bse1I ACTGG 5 cut(s) 283, 377, 484, 600, 743
Bse3DI GCAATG 2 cut(s) 78, 104
BseBI CCWGG 3 cut(s) 128, 347, 623
BseDI CCNNGG 3 cut(s) 45, 54, 622
BseGI GGATG 4 cut(s) 108, 308, 637, 991
BseLI CCNNNNNNNGG 7 cut(s) 346, 622, 623, 628, 849, 882, 982
BseMI GCAATG 2 cut(s) 78, 104
BseMII CTCAG 2 cut(s) 280, 492
BseNI ACTGG 5 cut(s) 283, 377, 484, 600, 743
BseXI GCAGC 5 cut(s) 70, 461, 527, 652, 655
BseYI CCCAGC 1 cut(s) 429
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 483, 975
BshNI GGYRCC 1 cut(s) 872
BsiHKAI GWGCWC 2 cut(s) 783, 908
BsiSI CCGG 2 cut(s) 652, 789
BsiWI CGTACG 1 cut(s) 330
BslFI GGGAC 3 cut(s) 64, 832, 871
BslI CCNNNNNNNGG 7 cut(s) 346, 622, 623, 628, 849, 882, 982
BsmAI GTCTC 1 cut(s) 751
BsmFI GGGAC 3 cut(s) 64, 832, 871
BsnI GGCC 2 cut(s) 483, 975
Bsp1286I GDGCHC 2 cut(s) 783, 908
Bsp143I GATC 1 cut(s) 489
Bsp1720I GCTNAGC 1 cut(s) 224
BspACI CCGC 3 cut(s) 312, 350, 792
BspANI GGCC 2 cut(s) 483, 975
BspCNI CTCAG 2 cut(s) 279, 493
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 2 cut(s) 52, 874
BspMAI CTGCAG 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 872
BsrDI GCAATG 2 cut(s) 78, 104
BsrI ACTGG 5 cut(s) 283, 377, 484, 600, 743
BssECI CCNNGG 3 cut(s) 45, 54, 622
BssMI GATC 1 cut(s) 489
BssT1I CCWWGG 2 cut(s) 45, 54
Bst2UI CCWGG 3 cut(s) 128, 347, 623
Bst4CI ACNGT 4 cut(s) 200, 419, 498, 707
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 440
BstBAI YACGTR 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 794
BstDEI CTNAG 5 cut(s) 224, 266, 341, 501, 941
BstF5I GGATG 4 cut(s) 108, 308, 637, 991
BstFNI CGCG 1 cut(s) 120
BstHHI GCGC 2 cut(s) 120, 137
BstKTI GATC 1 cut(s) 492
BstMAI GTCTC 1 cut(s) 751
BstMBI GATC 1 cut(s) 489
BstMWI GCNNNNNNNGC 6 cut(s) 65, 440, 449, 761, 770, 986
BstNI CCWGG 3 cut(s) 128, 347, 623
BstNSI RCATGY 1 cut(s) 642
BstSCI CCNGG 4 cut(s) 126, 345, 621, 651
BstSFI CTRYAG 1 cut(s) 441
BstSNI TACGTA 1 cut(s) 330
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 5 cut(s) 70, 461, 527, 652, 655
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 2 cut(s) 483, 975
BtrI CACGTC 1 cut(s) 909
BtsCI GGATG 4 cut(s) 108, 308, 637, 991
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 7 cut(s) 205, 294, 450, 494, 607, 712, 801
Cac8I GCNNGC 1 cut(s) 794
CfoI GCGC 2 cut(s) 120, 137
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 1 cut(s) 331
CviAII CATG 7 cut(s) 92, 254, 556, 639, 736, 900, 931
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 5 cut(s) 224, 266, 341, 501, 941
DpnI GATC 1 cut(s) 491
DpnII GATC 1 cut(s) 489
DraIII CACNNNGTG 1 cut(s) 912
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 781
Eco105I TACGTA 1 cut(s) 330
Eco130I CCWWGG 2 cut(s) 45, 54
Eco24I GRGCYC 1 cut(s) 783
Eco47I GGWCC 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 885
EcoICRI GAGCTC 1 cut(s) 781
EcoO109I RGGNCCY 1 cut(s) 51
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 3 cut(s) 126, 345, 621
EcoT14I CCWWGG 2 cut(s) 45, 54
EcoT22I ATGCAT 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 2 cut(s) 45, 54
FaeI CATG 7 cut(s) 95, 257, 559, 642, 739, 903, 934
FaqI GGGAC 3 cut(s) 64, 832, 871
FatI CATG 7 cut(s) 91, 253, 555, 638, 735, 899, 930
FauI CCCGC 1 cut(s) 785
Fnu4HI GCNGC 6 cut(s) 84, 351, 450, 541, 666, 669
FokI GGATG 4 cut(s) 115, 295, 644, 998
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 6 cut(s) 84, 351, 450, 541, 666, 669
GlaI GCGC 2 cut(s) 119, 136
GluI GCNGC 6 cut(s) 84, 351, 450, 541, 666, 669
GsaI CCCAGC 1 cut(s) 433
GsuI CTGGAG 1 cut(s) 394
HaeIII GGCC 2 cut(s) 483, 975
HapII CCGG 2 cut(s) 652, 789
HhaI GCGC 2 cut(s) 120, 137
Hin1II CATG 7 cut(s) 95, 257, 559, 642, 739, 903, 934
Hin6I GCGC 2 cut(s) 118, 135
HinP1I GCGC 2 cut(s) 118, 135
HinfI GANTC 2 cut(s) 299, 937
HpaII CCGG 2 cut(s) 652, 789
HphI GGTGA 3 cut(s) 34, 563, 929
Hpy166II GTNNAC 2 cut(s) 124, 801
Hpy188I TCNGA 4 cut(s) 244, 269, 502, 631
Hpy188III TCNNGA 2 cut(s) 166, 892
Hpy8I GTNNAC 2 cut(s) 124, 801
Hpy99I CGWCG 1 cut(s) 913
HpyAV CCTTC 4 cut(s) 136, 197, 452, 956
HpyCH4III ACNGT 4 cut(s) 200, 419, 498, 707
HpyCH4IV ACGT 3 cut(s) 71, 329, 908
HpyCH4V TGCA 6 cut(s) 86, 320, 443, 572, 689, 739
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 440, 449, 761, 770, 986
HpyF3I CTNAG 5 cut(s) 224, 266, 341, 501, 941
HpySE526I ACGT 3 cut(s) 71, 329, 908
Hsp92II CATG 7 cut(s) 95, 257, 559, 642, 739, 903, 934
HspAI GCGC 2 cut(s) 118, 135
Kzo9I GATC 1 cut(s) 489
LmnI GCTCC 1 cut(s) 903
Lsp1109I GCAGC 5 cut(s) 70, 461, 527, 652, 655
LweI GCATC 7 cut(s) 68, 237, 307, 329, 376, 943, 976
MaeII ACGT 3 cut(s) 71, 329, 908
MaeIII GTNAC 6 cut(s) 281, 391, 530, 551, 694, 838
MalI GATC 1 cut(s) 491
MboI GATC 1 cut(s) 489
MboII GAAGA 4 cut(s) 220, 269, 878, 946
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 2 cut(s) 783, 908
MlsI TGGCCA 1 cut(s) 483
MluNI TGGCCA 1 cut(s) 483
MnlI CCTC 6 cut(s) 316, 369, 584, 655, 775, 986
Mox20I TGGCCA 1 cut(s) 483
Mph1103I ATGCAT 1 cut(s) 322
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 1 cut(s) 483
MseI TTAA 2 cut(s) 23, 887
Msp20I TGGCCA 1 cut(s) 483
MspI CCGG 2 cut(s) 652, 789
MspR9I CCNGG 4 cut(s) 128, 347, 623, 653
MunI CAATTG 1 cut(s) 485
MvaI CCWGG 3 cut(s) 128, 347, 623
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 6 cut(s) 65, 440, 449, 761, 770, 986
NciI CCSGG 1 cut(s) 653
NdeII GATC 1 cut(s) 489
NlaIII CATG 7 cut(s) 95, 257, 559, 642, 739, 903, 934
NlaIV GGNNCC 2 cut(s) 52, 874
NmuCI GTSAC 1 cut(s) 551
NsiI ATGCAT 1 cut(s) 322
NspI RCATGY 1 cut(s) 642
PciI ACATGT 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 299, 937
Pfl23II CGTACG 1 cut(s) 330
PkrI GCNGC 6 cut(s) 85, 352, 451, 542, 667, 670
Ppu21I YACGTR 1 cut(s) 330
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 126, 345, 621
PspFI CCCAGC 1 cut(s) 429
PspGI CCWGG 3 cut(s) 126, 345, 621
PspLI CGTACG 1 cut(s) 330
PspN4I GGNNCC 2 cut(s) 52, 874
PspPI GGNCC 1 cut(s) 51
PspPPI RGGWCCY 1 cut(s) 51
PstI CTGCAG 1 cut(s) 445
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SacI GAGCTC 1 cut(s) 783
SaqAI TTAA 2 cut(s) 23, 887
SatI GCNGC 6 cut(s) 84, 351, 450, 541, 666, 669
Sau3AI GATC 1 cut(s) 489
Sau96I GGNCC 1 cut(s) 51
ScrFI CCNGG 4 cut(s) 128, 347, 623, 653
SduI GDGCHC 2 cut(s) 783, 908
SfaNI GCATC 7 cut(s) 68, 237, 307, 329, 376, 943, 976
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 63, 722
SmoI CTYRAG 2 cut(s) 63, 722
SnaBI TACGTA 1 cut(s) 330
SsiI CCGC 3 cut(s) 312, 350, 792
SstI GAGCTC 1 cut(s) 783
StyD4I CCNGG 4 cut(s) 126, 345, 621, 651
StyI CCWWGG 2 cut(s) 45, 54
TaaI ACNGT 4 cut(s) 200, 419, 498, 707
TaiI ACGT 3 cut(s) 74, 332, 911
TaqI TCGA 1 cut(s) 456
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 2 cut(s) 299, 937
Tru1I TTAA 2 cut(s) 23, 887
Tru9I TTAA 2 cut(s) 23, 887
TscAI CASTG 7 cut(s) 205, 301, 450, 501, 607, 712, 808
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 5 cut(s) 83, 449, 540, 665, 668
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 3 cut(s) 270, 662, 947
TspGWI ACGGA 2 cut(s) 603, 739
TspRI CASTG 7 cut(s) 205, 301, 450, 501, 607, 712, 808
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 2 cut(s) 179, 191
XceI RCATGY 1 cut(s) 642
XcmI CCANNNNNNNNNTGG 2 cut(s) 369, 629
XmnI GAANNNNTTC 1 cut(s) 261
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.