MD05G1288200.v1.1

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000341
Physical Location & Seq
Reverse (-)
42089181 .. 42090540
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1288200.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGCTTTTGAAAGAAACATTTTAATTGTTGCCATACTCATCACCTTGGGGACCTTGGCATCTCAAGCCACGTCCCGCACATTGTACGAAGATTCCATTGCTGCAAAACATGAGCAATGGATGGAAGAGTTTGGGCGCGTTTACCAGGACAGCGCAGAGAAGGAAAGGCGTTTCCACATATTCAAGAACAATGTGGAATTCGTGGAGAAATTCAACAGTGAAGGGAAGAAGACATACAAGCTAAGCATCAATCAATTTTCTGATATGACCCATGAAGAATTTCTCAGACATCATACTGGATACAAGAAGCCCACTGAATCAACCTCATCCGCAGATGCATCTTTTACGCACAAAAACCTAAGCCTGACGGATGTTCCCCCAAGCATTGACTGGAGGGAGCAAGATGCTGTTACTCCTGTAAAAGATCAAGGGAACTGTGGTTGTTGCTGGGCATTTTCTGCAGTGGCAGCAGTCGAAGGGATTACCAAAATCAAAACTGGCCAATTGATATCACTGTCTGAGCAACAACTTGTGGACTGTGATACAAGTAACTTTGGCTGCCAAGGTGGTGACATGGCTAAAGCCTTTGCATACATTCAACAGAACGGAGGAATTGCCAGTGAAGAAAATTACCCATACCAGGGTTCGGATGGAACATGTGATACGAACCTGGGAAATGAGGCTGCTGCCAAGATAACTAATTATGCAAGGGTAACTCCCAACAGTGAAACCGATTTGCTCAAGGCTGTATCCATGCAACCAGTCTCCGTTGCCATCGCTGCCTCACAAGCCTTTCGGCTCTATTCCGGGGGCCTGTTCACTGATGATAGTTGTGGGACAGAATTGAACCACGCTGTTACCATCATTGGGTATGGGACAACTGAAGATGGCACCCCGTATTGGTTAATCAAGAACTCATGGGCCGAAAGGTGGGGTGATAATGGTTACATGAAGATTCTTAGAAACGCCGATGCCCCAGAAGGTGTTTGTGGCCTCGCTATGGAAGCTTCCTATCCAATTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

342

Amino Acids

37.57

Weight (kDa)

4.92

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 37 - 94 3.9e-20 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 125 - 340 7.8e-82 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 890
AccII CGCG 1 cut(s) 138
AciI CCGC 2 cut(s) 76, 330
AcoI YGGCCR 1 cut(s) 499
AcsI RAATTY 3 cut(s) 197, 209, 278
AcuI CTGAAG 1 cut(s) 903
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 7 cut(s) 640, 641, 646, 867, 900, 930, 1000
AflIII ACRYGT 1 cut(s) 656
AgsI TTSAA 5 cut(s) 11, 184, 214, 599, 847
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 3 cut(s) 144, 639, 669
AluBI AGCT 2 cut(s) 241, 1007
AluI AGCT 2 cut(s) 241, 1007
Alw26I GTCTC 1 cut(s) 769
AoxI GGCC 4 cut(s) 499, 811, 921, 991
ApeKI GCWGC 6 cut(s) 101, 467, 558, 683, 686, 779
ApoI RAATTY 3 cut(s) 197, 209, 278
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 2 cut(s) 138, 155
AspS9I GGNCC 3 cut(s) 51, 811, 921
AsuC2I CCSGG 1 cut(s) 808
AsuHPI GGTGA 3 cut(s) 34, 581, 947
AvaII GGWCC 1 cut(s) 51
BalI TGGCCA 1 cut(s) 501
BanI GGYRCC 1 cut(s) 890
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BbsI GAAGAC 1 cut(s) 236
BbvI GCAGC 6 cut(s) 88, 479, 545, 670, 673, 766
BccI CCATC 5 cut(s) 115, 644, 782, 869, 881
BciT130I CCWGG 3 cut(s) 146, 641, 671
BciVI GTATCC 2 cut(s) 293, 760
BcnI CCSGG 1 cut(s) 808
BcoDI GTCTC 1 cut(s) 769
BfmI CTRYAG 1 cut(s) 459
BfuI GTATCC 2 cut(s) 293, 760
BisI GCNGC 6 cut(s) 102, 468, 559, 684, 687, 780
BlpI GCTNAGC 1 cut(s) 242
BlsI GCNGC 6 cut(s) 103, 469, 560, 685, 688, 781
Bme1390I CCNGG 4 cut(s) 146, 641, 671, 808
Bme18I GGWCC 1 cut(s) 51
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 51, 811, 921
BmiI GGNNCC 3 cut(s) 52, 812, 892
BmrFI CCNGG 4 cut(s) 146, 641, 671, 808
BmsI GCATC 6 cut(s) 68, 255, 325, 347, 394, 961
BpiI GAAGAC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 412
Bpu10I CCTNAGC 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 48, 725
BpuMI CCSGG 1 cut(s) 808
BsaJI CCNNGG 6 cut(s) 45, 54, 562, 640, 670, 807
Bsc4I CCNNNNNNNGG 7 cut(s) 640, 641, 646, 867, 900, 930, 1000
Bse1I ACTGG 5 cut(s) 301, 395, 502, 618, 761
Bse3DI GCAATG 2 cut(s) 96, 122
BseBI CCWGG 3 cut(s) 146, 641, 671
BseDI CCNNGG 6 cut(s) 45, 54, 562, 640, 670, 807
BseGI GGATG 4 cut(s) 126, 326, 376, 655
BseLI CCNNNNNNNGG 7 cut(s) 640, 641, 646, 867, 900, 930, 1000
BseMI GCAATG 2 cut(s) 96, 122
BseMII CTCAG 2 cut(s) 298, 510
BseNI ACTGG 5 cut(s) 301, 395, 502, 618, 761
BseXI GCAGC 6 cut(s) 88, 479, 545, 670, 673, 766
BseYI CCCAGC 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 4 cut(s) 501, 813, 923, 993
BshNI GGYRCC 1 cut(s) 890
BsiSI CCGG 1 cut(s) 807
BslFI GGGAC 4 cut(s) 58, 64, 850, 889
BslI CCNNNNNNNGG 7 cut(s) 640, 641, 646, 867, 900, 930, 1000
BsmAI GTCTC 1 cut(s) 769
BsmFI GGGAC 4 cut(s) 58, 64, 850, 889
BsnI GGCC 4 cut(s) 501, 813, 923, 993
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 242
BspACI CCGC 2 cut(s) 76, 330
BspANI GGCC 4 cut(s) 501, 813, 923, 993
BspCNI CTCAG 2 cut(s) 297, 511
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 3 cut(s) 52, 812, 892
BspMAI CTGCAG 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 890
BsrDI GCAATG 2 cut(s) 96, 122
BsrI ACTGG 5 cut(s) 301, 395, 502, 618, 761
BssECI CCNNGG 6 cut(s) 45, 54, 562, 640, 670, 807
BssMI GATC 1 cut(s) 424
BssT1I CCWWGG 3 cut(s) 45, 54, 562
Bst2UI CCWGG 3 cut(s) 146, 641, 671
Bst4CI ACNGT 5 cut(s) 218, 437, 516, 539, 725
Bst6I CTCTTC 1 cut(s) 120
BstAPI GCANNNNNTGC 1 cut(s) 458
BstDEI CTNAG 5 cut(s) 242, 284, 359, 519, 959
BstF5I GGATG 4 cut(s) 126, 326, 376, 655
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 2 cut(s) 138, 155
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 1 cut(s) 769
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 6 cut(s) 65, 458, 467, 779, 788, 1004
BstNI CCWGG 3 cut(s) 146, 641, 671
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 4 cut(s) 144, 639, 669, 806
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 6 cut(s) 88, 479, 545, 670, 673, 766
BstV2I GAAGAC 1 cut(s) 236
BsuI GTATCC 2 cut(s) 293, 760
BsuRI GGCC 4 cut(s) 501, 813, 923, 993
BtgZI GCGATG 1 cut(s) 760
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 4 cut(s) 126, 326, 376, 655
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 7 cut(s) 223, 312, 468, 512, 625, 730, 819
CfoI GCGC 2 cut(s) 138, 155
Cfr13I GGNCC 3 cut(s) 51, 811, 921
Csp6I GTAC 1 cut(s) 85
CviAII CATG 7 cut(s) 110, 272, 574, 657, 754, 918, 949
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 5 cut(s) 242, 284, 359, 519, 959
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 499
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 3 cut(s) 45, 54, 562
Eco32I GATATC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 903
EcoO109I RGGNCCY 2 cut(s) 51, 811
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 144, 639, 669
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 3 cut(s) 45, 54, 562
EcoT22I ATGCAT 1 cut(s) 340
ErhI CCWWGG 3 cut(s) 45, 54, 562
FaeI CATG 7 cut(s) 113, 275, 577, 660, 757, 921, 952
FaqI GGGAC 4 cut(s) 58, 64, 850, 889
FatI CATG 7 cut(s) 109, 271, 573, 656, 753, 917, 948
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 6 cut(s) 102, 468, 559, 684, 687, 780
FokI GGATG 4 cut(s) 133, 313, 383, 662
Fsp4HI GCNGC 6 cut(s) 102, 468, 559, 684, 687, 780
GlaI GCGC 2 cut(s) 137, 154
GluI GCNGC 6 cut(s) 102, 468, 559, 684, 687, 780
GsaI CCCAGC 1 cut(s) 451
GsuI CTGGAG 1 cut(s) 412
HaeIII GGCC 4 cut(s) 501, 813, 923, 993
HapII CCGG 1 cut(s) 807
HhaI GCGC 2 cut(s) 138, 155
Hin1II CATG 7 cut(s) 113, 275, 577, 660, 757, 921, 952
Hin6I GCGC 2 cut(s) 136, 153
HinP1I GCGC 2 cut(s) 136, 153
HindIII AAGCTT 1 cut(s) 1005
HinfI GANTC 3 cut(s) 92, 317, 955
HpaII CCGG 1 cut(s) 807
HphI GGTGA 3 cut(s) 34, 581, 947
Hpy166II GTNNAC 3 cut(s) 142, 535, 819
Hpy188I TCNGA 4 cut(s) 262, 287, 520, 649
Hpy188III TCNNGA 2 cut(s) 184, 910
Hpy8I GTNNAC 3 cut(s) 142, 535, 819
HpyAV CCTTC 4 cut(s) 154, 215, 470, 974
HpyCH4III ACNGT 5 cut(s) 218, 437, 516, 539, 725
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 6 cut(s) 104, 338, 461, 590, 707, 757
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 458, 467, 779, 788, 1004
HpyF3I CTNAG 5 cut(s) 242, 284, 359, 519, 959
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 7 cut(s) 113, 275, 577, 660, 757, 921, 952
HspAI GCGC 2 cut(s) 136, 153
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 1 cut(s) 397
Lsp1109I GCAGC 6 cut(s) 88, 479, 545, 670, 673, 766
LweI GCATC 6 cut(s) 68, 255, 325, 347, 394, 961
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 6 cut(s) 409, 548, 569, 712, 856, 944
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 8 cut(s) 101, 137, 238, 241, 287, 635, 896, 964
MfeI CAATTG 1 cut(s) 503
MlsI TGGCCA 1 cut(s) 501
MluNI TGGCCA 1 cut(s) 501
MnlI CCTC 6 cut(s) 334, 387, 602, 673, 793, 1004
Mox20I TGGCCA 1 cut(s) 501
Mph1103I ATGCAT 1 cut(s) 340
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 1 cut(s) 501
MseI TTAA 2 cut(s) 23, 905
Msp20I TGGCCA 1 cut(s) 501
MspI CCGG 1 cut(s) 807
MspR9I CCNGG 4 cut(s) 146, 641, 671, 808
MunI CAATTG 1 cut(s) 503
MvaI CCWGG 3 cut(s) 146, 641, 671
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 6 cut(s) 65, 458, 467, 779, 788, 1004
NciI CCSGG 1 cut(s) 808
NdeII GATC 1 cut(s) 424
NlaIII CATG 7 cut(s) 113, 275, 577, 660, 757, 921, 952
NlaIV GGNNCC 3 cut(s) 52, 812, 892
NmuCI GTSAC 1 cut(s) 569
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 660
PciI ACATGT 1 cut(s) 656
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 92, 317, 955
PkrI GCNGC 6 cut(s) 103, 469, 560, 685, 688, 781
PpuMI RGGWCCY 1 cut(s) 51
PscI ACATGT 1 cut(s) 656
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 144, 639, 669
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 3 cut(s) 144, 639, 669
PspN4I GGNNCC 3 cut(s) 52, 812, 892
PspPI GGNCC 3 cut(s) 51, 811, 921
PspPPI RGGWCCY 1 cut(s) 51
PsrI GAACNNNNNNTAC 2 cut(s) 646, 678
PstI CTGCAG 1 cut(s) 463
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
SaqAI TTAA 2 cut(s) 23, 905
SatI GCNGC 6 cut(s) 102, 468, 559, 684, 687, 780
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 3 cut(s) 51, 811, 921
ScrFI CCNGG 4 cut(s) 146, 641, 671, 808
SfaNI GCATC 6 cut(s) 68, 255, 325, 347, 394, 961
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 51
SmlI CTYRAG 2 cut(s) 63, 740
SmoI CTYRAG 2 cut(s) 63, 740
SsiI CCGC 2 cut(s) 76, 330
StyD4I CCNGG 4 cut(s) 144, 639, 669, 806
StyI CCWWGG 3 cut(s) 45, 54, 562
TaaI ACNGT 5 cut(s) 218, 437, 516, 539, 725
TaiI ACGT 1 cut(s) 74
TaqI TCGA 1 cut(s) 474
TfiI GAWTC 3 cut(s) 92, 317, 955
Tru1I TTAA 2 cut(s) 23, 905
Tru9I TTAA 2 cut(s) 23, 905
TscAI CASTG 7 cut(s) 223, 319, 468, 519, 625, 730, 826
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 6 cut(s) 101, 467, 558, 683, 686, 779
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 2 cut(s) 288, 965
TspGWI ACGGA 3 cut(s) 383, 621, 757
TspRI CASTG 7 cut(s) 223, 319, 468, 519, 625, 730, 826
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 3 cut(s) 197, 209, 278
XceI RCATGY 1 cut(s) 660
XcmI CCANNNNNNNNNTGG 2 cut(s) 387, 647
XmnI GAANNNNTTC 1 cut(s) 279
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.