Rh5AG167500

cysteine-type peptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000341
Physical Location & Seq
Forward (+)
18208763 .. 18209804
1042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG167500.1

Sequence Viewer

Length: 918 bp
ATGAGCAATGGATGGCCGAGTATGGGCACAGAGAAGGAAAGACGTTTTGCCATATTCAAGAAGAATGTAGAGTTTGTGGAGAAATTCAACAATGACAGGGAAAAGACGTACAAGTTAAGCGTCAATCAGTTCTCTGATATGACCAGTGAAGAATTCCGCAGACAACATACTGGATACAAAACTCCCGCCGGCTCCAGTTTAACATCCTCATCCGAAGATAAGTCCTTCACATACCGAAGCCTGTCCATTGATGTTATTCCAACTAGCCTTGACTGGAGGGAACAAGGAGCCGTCACCTCTATTAAGGATCAAGGTTCATGTACTGTTTGCTGGGCATTTGCTGCGGCGGCAGCTGTGGAAGGGATTACGAAAATCAAAACCGGCCAATTGGTCTCGCTGTCCGAGCAACAACTTTTGGAGTGTGACCGCAACATTGGCTGCGAAAGTGGTAGCGTCGAATCAGCTTTTGAATACATAAAGACAAATGGAGGAATTGCTAGTGAAGAAAAGTACCCATACCTGAGCACTTCTAATGTGTCAACGGTCCGAAAATGCGACACTCAAAAGGAAACTATGCATGCTGCCCGGATAACTGGTTATAAAAATGTACCATCCTACAATCAAAGTGCTCTACTCGAGGCTGTTTCCATGCAACCAGTCTCGACTGCCATCGAGGGTCATGGAAGGGAATTTCAGTTATACAGAGGTGGAGTGTTTAATGGTTCTTGCGGTCACATACTGAGCCATGCTGTCACAATTATTGGGTACGGGACTGAAAACGGCACCAACTACTGGCTAATTAAGAATTCATGGGGGGAGAAGTGGGGTGAAAAGGGCTACATGAGAATTCTTAGAACTGAAACATATGGTGCAGGTCTTTGTGGCCTCGCTAAGCAAGCTTCCTATCCGATTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko04621 ko04924 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map04621 map04924 map05205 map05323 map05418
Pfam Domains
Protein Families
Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

305

Amino Acids

33.83

Weight (kDa)

6.96

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I29 PF08246 11 - 52 1.2e-13 Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1 PF00112 86 - 304 2.9e-74 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 782
AccB7I CCANNNNNTGG 1 cut(s) 792
AciI CCGC 6 cut(s) 157, 186, 344, 347, 427, 729
AclWI GGATC 1 cut(s) 315
AcoI YGGCCR 2 cut(s) 14, 382
AcsI RAATTY 5 cut(s) 83, 152, 689, 805, 846
AfaI GTAC 5 cut(s) 110, 322, 512, 609, 767
AfiI CCNNNNNNNGG 2 cut(s) 23, 792
AgsI TTSAA 4 cut(s) 58, 88, 470, 914
AluBI AGCT 3 cut(s) 353, 464, 899
AluI AGCT 3 cut(s) 353, 464, 899
Alw21I GWGCWC 2 cut(s) 527, 631
Alw26I GTCTC 2 cut(s) 397, 664
AlwI GGATC 1 cut(s) 315
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 3 cut(s) 14, 382, 883
ApeKI GCWGC 4 cut(s) 341, 350, 438, 581
ApoI RAATTY 5 cut(s) 83, 152, 689, 805, 846
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 1 cut(s) 586
AsuHPI GGTGA 2 cut(s) 286, 839
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 782
Bbv12I GWGCWC 2 cut(s) 527, 631
BbvI GCAGC 4 cut(s) 328, 362, 425, 568
BccI CCATC 3 cut(s) 6, 619, 677
BceAI ACGGC 2 cut(s) 275, 796
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 586
BcoDI GTCTC 2 cut(s) 397, 664
BfaI CTAG 2 cut(s) 264, 498
BfuAI ACCTGC 1 cut(s) 863
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 6 cut(s) 342, 345, 348, 351, 439, 582
BlpI GCTNAGC 1 cut(s) 891
BlsI GCNGC 6 cut(s) 343, 346, 349, 352, 440, 583
Bme1390I CCNGG 1 cut(s) 586
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 3 cut(s) 193, 289, 784
BmrFI CCNGG 1 cut(s) 586
BpmI CTGGAG 2 cut(s) 178, 295
Bpu10I CCTNAGC 1 cut(s) 521
Bpu1102I GCTNAGC 1 cut(s) 891
BpuMI CCSGG 1 cut(s) 586
BsaI GGTCTC 1 cut(s) 397
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 792
Bse118I RCCGGY 2 cut(s) 188, 380
Bse1I ACTGG 7 cut(s) 144, 175, 195, 278, 598, 656, 797
Bse3DI GCAATG 1 cut(s) 13
BseGI GGATG 4 cut(s) 17, 203, 209, 611
BseLI CCNNNNNNNGG 2 cut(s) 23, 792
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 512, 731
BseNI ACTGG 7 cut(s) 144, 175, 195, 278, 598, 656, 797
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 4 cut(s) 328, 362, 425, 568
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 891
BshFI GGCC 3 cut(s) 16, 384, 885
BshNI GGYRCC 1 cut(s) 782
BsiHKAI GWGCWC 2 cut(s) 527, 631
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 3 cut(s) 189, 381, 586
BslFI GGGAC 1 cut(s) 784
BslI CCNNNNNNNGG 2 cut(s) 23, 792
BsmAI GTCTC 2 cut(s) 397, 664
BsmFI GGGAC 1 cut(s) 784
BsnI GGCC 3 cut(s) 16, 384, 885
Bso31I GGTCTC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 29, 527, 631
Bsp143I GATC 1 cut(s) 307
Bsp1720I GCTNAGC 1 cut(s) 891
BspACI CCGC 6 cut(s) 157, 186, 344, 347, 427, 729
BspANI GGCC 3 cut(s) 16, 384, 885
BspCNI CTCAG 2 cut(s) 513, 732
BspLI GGNNCC 3 cut(s) 193, 289, 784
BspMI ACCTGC 1 cut(s) 863
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 397
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 2 cut(s) 188, 380
BsrI ACTGG 7 cut(s) 144, 175, 195, 278, 598, 656, 797
BssAI RCCGGY 2 cut(s) 188, 380
BssMI GATC 1 cut(s) 307
Bst4CI ACNGT 2 cut(s) 325, 544
BstAPI GCANNNNNTGC 1 cut(s) 341
BstC8I GCNNGC 3 cut(s) 190, 579, 897
BstDEI CTNAG 4 cut(s) 521, 740, 851, 891
BstF5I GGATG 4 cut(s) 17, 203, 209, 611
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 2 cut(s) 397, 664
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 6 cut(s) 341, 347, 350, 403, 435, 896
BstNSI RCATGY 1 cut(s) 581
BstSCI CCNGG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 29
BstV1I GCAGC 4 cut(s) 328, 362, 425, 568
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 3 cut(s) 16, 384, 885
BtsCI GGATG 4 cut(s) 17, 203, 209, 611
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 863
Cac8I GCNNGC 3 cut(s) 190, 579, 897
Cfr10I RCCGGY 2 cut(s) 188, 380
Cfr13I GGNCC 1 cut(s) 544
CpoI CGGWCCG 1 cut(s) 544
CseI GACGC 2 cut(s) 109, 442
Csp6I GTAC 5 cut(s) 109, 321, 511, 608, 766
CspI CGGWCCG 1 cut(s) 544
CviAII CATG 7 cut(s) 318, 578, 649, 680, 746, 810, 841
CviQI GTAC 5 cut(s) 109, 321, 511, 608, 766
DdeI CTNAG 4 cut(s) 521, 740, 851, 891
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 14, 382
Eco31I GGTCTC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 635
EcoRI GAATTC 3 cut(s) 152, 805, 846
EcoT22I ATGCAT 1 cut(s) 579
FaeI CATG 7 cut(s) 321, 581, 652, 683, 749, 813, 844
FaqI GGGAC 1 cut(s) 784
FatI CATG 7 cut(s) 317, 577, 648, 679, 745, 809, 840
FauI CCCGC 1 cut(s) 193
FauNDI CATATG 1 cut(s) 865
Fnu4HI GCNGC 6 cut(s) 342, 345, 348, 351, 439, 582
FokI GGATG 4 cut(s) 24, 190, 196, 598
Fsp4HI GCNGC 6 cut(s) 342, 345, 348, 351, 439, 582
FspBI CTAG 2 cut(s) 264, 498
GluI GCNGC 6 cut(s) 342, 345, 348, 351, 439, 582
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 2 cut(s) 178, 295
HaeIII GGCC 3 cut(s) 16, 384, 885
HapII CCGG 3 cut(s) 189, 381, 586
HgaI GACGC 2 cut(s) 109, 442
Hin1II CATG 7 cut(s) 321, 581, 652, 683, 749, 813, 844
HincII GTYRAC 1 cut(s) 540
HindII GTYRAC 1 cut(s) 540
HindIII AAGCTT 1 cut(s) 897
HinfI GANTC 1 cut(s) 458
HpaII CCGG 3 cut(s) 189, 381, 586
HphI GGTGA 2 cut(s) 286, 839
Hpy166II GTNNAC 1 cut(s) 540
Hpy188I TCNGA 5 cut(s) 136, 214, 403, 548, 909
Hpy188III TCNNGA 2 cut(s) 58, 661
Hpy8I GTNNAC 1 cut(s) 540
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 4 cut(s) 28, 235, 353, 678
HpyCH4III ACNGT 2 cut(s) 325, 544
HpyCH4IV ACGT 2 cut(s) 43, 107
HpyCH4V TGCA 3 cut(s) 577, 652, 872
HpyF10VI GCNNNNNNNGC 6 cut(s) 341, 347, 350, 403, 435, 896
HpyF3I CTNAG 4 cut(s) 521, 740, 851, 891
HpySE526I ACGT 2 cut(s) 43, 107
Hsp92II CATG 7 cut(s) 321, 581, 652, 683, 749, 813, 844
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 197, 287
Lsp1109I GCAGC 4 cut(s) 328, 362, 425, 568
MaeI CTAG 2 cut(s) 264, 498
MaeII ACGT 2 cut(s) 43, 107
MaeIII GTNAC 4 cut(s) 292, 422, 731, 751
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 4 cut(s) 73, 161, 227, 515
MfeI CAATTG 1 cut(s) 386
MhlI GDGCHC 3 cut(s) 29, 527, 631
MluCI AATT 9 cut(s) 83, 152, 386, 492, 689, 756, 798, 805, 846
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 8 cut(s) 217, 270, 307, 482, 631, 667, 698, 896
Mph1103I ATGCAT 1 cut(s) 579
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 5 cut(s) 116, 200, 303, 717, 801
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 3 cut(s) 189, 381, 586
MspR9I CCNGG 1 cut(s) 586
MunI CAATTG 1 cut(s) 386
MwoI GCNNNNNNNGC 6 cut(s) 341, 347, 350, 403, 435, 896
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 586
NdeI CATATG 1 cut(s) 865
NdeII GATC 1 cut(s) 307
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 7 cut(s) 321, 581, 652, 683, 749, 813, 844
NlaIV GGNNCC 3 cut(s) 193, 289, 784
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 4 cut(s) 292, 422, 731, 751
NsiI ATGCAT 1 cut(s) 579
NspI RCATGY 1 cut(s) 581
PaeI GCATGC 1 cut(s) 581
PaeR7I CTCGAG 1 cut(s) 635
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 792
PkrI GCNGC 6 cut(s) 343, 346, 349, 352, 440, 583
PsiI TTATAA 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 193, 289, 784
PspPI GGNCC 1 cut(s) 544
PspXI VCTCGAGB 1 cut(s) 635
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 5 cut(s) 110, 322, 512, 609, 767
RsaNI GTAC 5 cut(s) 109, 321, 511, 608, 766
Rsr2I CGGWCCG 1 cut(s) 544
RsrII CGGWCCG 1 cut(s) 544
SaqAI TTAA 5 cut(s) 116, 200, 303, 717, 801
SatI GCNGC 6 cut(s) 342, 345, 348, 351, 439, 582
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 1 cut(s) 544
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 3 cut(s) 29, 527, 631
Sfr274I CTCGAG 1 cut(s) 635
SinI GGWCC 1 cut(s) 544
SlaI CTCGAG 1 cut(s) 635
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
SphI GCATGC 1 cut(s) 581
Sse9I AATT 9 cut(s) 83, 152, 386, 492, 689, 756, 798, 805, 846
SsiI CCGC 6 cut(s) 157, 186, 344, 347, 427, 729
SspMI CTAG 2 cut(s) 264, 498
StyD4I CCNGG 1 cut(s) 584
TaaI ACNGT 2 cut(s) 325, 544
TaiI ACGT 2 cut(s) 46, 110
TaqI TCGA 4 cut(s) 456, 636, 662, 672
TasI AATT 9 cut(s) 83, 152, 386, 492, 689, 756, 798, 805, 846
TatI WGTACW 1 cut(s) 320
TauI GCSGC 2 cut(s) 347, 350
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 5 cut(s) 116, 200, 303, 717, 801
Tru9I TTAA 5 cut(s) 116, 200, 303, 717, 801
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 4 cut(s) 292, 422, 731, 751
TseI GCWGC 4 cut(s) 341, 350, 438, 581
Tsp45I GTSAC 4 cut(s) 292, 422, 731, 751
TspDTI ATGAA 2 cut(s) 306, 798
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 792
VpaK11BI GGWCC 1 cut(s) 544
XapI RAATTY 5 cut(s) 83, 152, 689, 805, 846
XceI RCATGY 1 cut(s) 581
XhoI CTCGAG 1 cut(s) 635
XspI CTAG 2 cut(s) 264, 498
Zsp2I ATGCAT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.