RchiOBHm_Chr5g0023621

Zingipain-2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000341
Physical Location & Seq
Forward (+)
17684393 .. 17685001
609 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30348

Sequence Viewer

Length: 609 bp
ATGATATGTGTAGCTGTTTGCTGGGCATTTGCTGCGGCGGCAGCAGTGGAAGGGATTACGAAAATCAAAACCGGCCAATTGGTCTCGCTGTCCGAGCAACAACTTTTGGAGTGTGACCGCAACATTGGCTGCGAAAGTGGTAGCGTCCGATCAGCTTTTGAATACATAAAGACAAATGGAGGAATTGCTAGTGAAGAAAAGTACCCATACCTGAGCACTTCTAATGTGTCAACGGTCCGAAAATGCGACACTCAAAAGGAAACTATGCATGCTGCCCGGATAACTGGTTATAAAAATGTACCATCCAACAATCAAAGTGCTCTACTCGAGGCTGTTTCCATGCAACCAGTCTCGACTGCCATCGAGGGTCATGGAAGGGAATTTCAGTTATACAGAGGTGGAGTGTTTAATGGTTCTTGCGGTCACATACTGACCCATGCTGTCACAATTATTGGGTACGGGACTGAAAATGGCACCAACTACTGGCTAATTAAGAATTCATGGGGGGAGAAGTGGGGTGAAAAGGGCTACATGAGAATTCTTAGAACTGAAACATATGGTGCAGGTCTTTGTGGCCTCGCTAAGCATGCTTCCTATCCGATTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04140 ko04142 ko04145 ko04210 ko04612 ko05205 ko05323 ko05418 map04140 map04142 map04145 map04210 map04612 map05205 map05323 map05418
Pfam Domains
Protein Families
Inhibitor_I29 Peptidase_C1

Protein Analysis

202

Amino Acids

21.96

Weight (kDa)

8.13

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C1 PF00112 6 - 201 1e-63 Papain family cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000341)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27420 AT3G49340
fragaria_vesca FvH4_3g07960 FvH4_3g07970 FvH4_3g08030 FvH4_3g14350 FvH4_3g14380 FvH4_3g20730
malus_domestica MD04G1190600.v1.1 MD05G1287600.v1.1 MD05G1287900.v1.1 MD05G1288200.v1.1 MD05G1288400.v1.1 MD05G1302800.v1.1 MD10G1281600.v1.1 MD10G1281700.v1.1 MD10G1281800.v1.1 MD10G1282100.v1.1 MD10G1282200.v1.1 MD16G1031600.v1.1 MD16G1032300.v1.1
prunus_persica Prupe.4G059600_v2.0.a1
pyrus_communis pycom10g23500 pycom10g23510 pycom10g23520
rosa_chinensis RchiOBHm_Chr5g0010261 RchiOBHm_Chr5g0010361 RchiOBHm_Chr5g0010391 RchiOBHm_Chr5g0010401 RchiOBHm_Chr5g0023511 RchiOBHm_Chr5g0023601 RchiOBHm_Chr5g0023621 RchiOBHm_Chr5g0023691 RchiOBHm_Chr5g0033681 RchiOBHm_Chr5g0034901 RchiOBHm_Chr5g0034951 RchiOBHm_Chr5g0067391
rosa_laevigata RLG00000016148 RLG00000031732 RLG00000031742 RLG00000031743 RLG00000031748 RLG00000031751 RLG00000031752 RLG00000031754 RLG00000032725 RLG00000032730 RLG00000033542 RLG00000033584 RLG00000033587 RLG00000035932
rosa_multiflora Rmu_co7996926.1_g000001 Rmu_co8207458.1_g000001 Rmu_co8273509.1_g000001 Rmu_sc0000062.1_g000016 Rmu_sc0000062.1_g000017 Rmu_sc0001580.1_g000004 Rmu_sc0001580.1_g000023 Rmu_sc0007816.1_g000005 Rmu_sc0007816.1_g000009 Rmu_sc0008877.1_g000003 Rmu_sc0009313.1_g000011 Rmu_sc0009313.1_g000015 Rmu_sc0009313.1_g000021
rosa_roxburghii Rroxscaffold_1G00012970 Rroxscaffold_1G00045430 Rroxscaffold_1G00055550 Rroxscaffold_1G00065850 Rroxscaffold_1G00065880 Rroxscaffold_1G00065890 Rroxscaffold_1G00065900 Rroxscaffold_1G00065990
rosa_rugosa Rorug04G0450100 Rorug04G0450100 Rorug04G0450200 Rorug04G0451200 Rorug05G0145700 Rorug05G0149000 Rorug05G0385000
rosa_samantha Rh5AG080800 Rh5AG081500 Rh5AG081700 Rh5AG081900 Rh5AG167500 Rh5AG168500 Rh5AG232900 Rh5AG241400 Rh5AG242000 Rh5AG444500 Rh5BG075300 Rh5BG076500 Rh5BG076600 Rh5BG077000 Rh5BG165800 Rh5BG237800 Rh5BG241500 Rh5BG241600 Rh5BG461200 Rh5BG461300
rosa_wichuraiana Rw0G000780 Rw0G021930 Rw5G007480 Rw5G007560 Rw5G007570 Rw5G015200 Rw5G015260 Rw5G022090 Rw5G022100 Rw5G041490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 473
AccB7I CCANNNNNTGG 1 cut(s) 483
AciI CCGC 4 cut(s) 35, 38, 118, 420
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 3 cut(s) 380, 496, 537
AfaI GTAC 3 cut(s) 203, 300, 458
AfiI CCNNNNNNNGG 1 cut(s) 483
AgsI TTSAA 1 cut(s) 161
AluBI AGCT 2 cut(s) 14, 155
AluI AGCT 2 cut(s) 14, 155
Alw21I GWGCWC 2 cut(s) 218, 322
Alw26I GTCTC 2 cut(s) 88, 355
Ama87I CYCGRG 1 cut(s) 326
AoxI GGCC 2 cut(s) 73, 574
ApeKI GCWGC 4 cut(s) 32, 41, 129, 272
ApoI RAATTY 3 cut(s) 380, 496, 537
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 326
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 473
Bbv12I GWGCWC 2 cut(s) 218, 322
BbvI GCAGC 4 cut(s) 19, 53, 116, 259
BccI CCATC 2 cut(s) 310, 368
BcnI CCSGG 1 cut(s) 277
BcoDI GTCTC 2 cut(s) 88, 355
BfaI CTAG 1 cut(s) 189
BfuAI ACCTGC 1 cut(s) 554
BisI GCNGC 6 cut(s) 33, 36, 39, 42, 130, 273
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 6 cut(s) 34, 37, 40, 43, 131, 274
Bme1390I CCNGG 1 cut(s) 277
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 1 cut(s) 277
Bpu10I CCTNAGC 1 cut(s) 212
Bpu1102I GCTNAGC 1 cut(s) 582
BpuMI CCSGG 1 cut(s) 277
BsaI GGTCTC 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 483
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 289, 347, 488
BseGI GGATG 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 3 cut(s) 289, 347, 488
BseXI GCAGC 4 cut(s) 19, 53, 116, 259
BseYI CCCAGC 1 cut(s) 21
BsgI GTGCAG 1 cut(s) 582
BshFI GGCC 2 cut(s) 75, 576
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 2 cut(s) 218, 322
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 2 cut(s) 72, 277
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 1 cut(s) 483
BsmAI GTCTC 2 cut(s) 88, 355
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 2 cut(s) 75, 576
Bso31I GGTCTC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 218, 322
Bsp143I GATC 1 cut(s) 149
Bsp1720I GCTNAGC 1 cut(s) 582
BspACI CCGC 4 cut(s) 35, 38, 118, 420
BspANI GGCC 2 cut(s) 75, 576
BspCNI CTCAG 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 475
BspMI ACCTGC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 3 cut(s) 289, 347, 488
BssAI RCCGGY 1 cut(s) 71
BssMI GATC 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 270, 588
BstDEI CTNAG 3 cut(s) 212, 542, 582
BstF5I GGATG 1 cut(s) 302
BstKTI GATC 1 cut(s) 152
BstMAI GTCTC 2 cut(s) 88, 355
BstMBI GATC 1 cut(s) 149
BstMWI GCNNNNNNNGC 6 cut(s) 32, 38, 41, 94, 126, 587
BstNSI RCATGY 2 cut(s) 272, 590
BstSCI CCNGG 1 cut(s) 275
BstV1I GCAGC 4 cut(s) 19, 53, 116, 259
BsuRI GGCC 2 cut(s) 75, 576
BtsCI GGATG 1 cut(s) 302
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 554
Cac8I GCNNGC 2 cut(s) 270, 588
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 235
CpoI CGGWCCG 1 cut(s) 235
CseI GACGC 1 cut(s) 133
Csp6I GTAC 3 cut(s) 202, 299, 457
CspI CGGWCCG 1 cut(s) 235
CviAII CATG 7 cut(s) 269, 340, 371, 437, 501, 532, 587
CviJI RGCY 8 cut(s) 14, 75, 129, 155, 332, 487, 528, 576
CviKI_1 RGCY 8 cut(s) 14, 75, 129, 155, 332, 487, 528, 576
CviQI GTAC 3 cut(s) 202, 299, 457
DdeI CTNAG 3 cut(s) 212, 542, 582
DpnI GATC 1 cut(s) 151
DpnII GATC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 326
EcoRI GAATTC 2 cut(s) 496, 537
EcoT22I ATGCAT 1 cut(s) 270
FaeI CATG 7 cut(s) 272, 343, 374, 440, 504, 535, 590
FaqI GGGAC 1 cut(s) 475
FatI CATG 7 cut(s) 268, 339, 370, 436, 500, 531, 586
FauNDI CATATG 1 cut(s) 556
Fnu4HI GCNGC 6 cut(s) 33, 36, 39, 42, 130, 273
FokI GGATG 1 cut(s) 289
Fsp4HI GCNGC 6 cut(s) 33, 36, 39, 42, 130, 273
FspBI CTAG 1 cut(s) 189
GluI GCNGC 6 cut(s) 33, 36, 39, 42, 130, 273
GsaI CCCAGC 1 cut(s) 25
HaeIII GGCC 2 cut(s) 75, 576
HapII CCGG 2 cut(s) 72, 277
HgaI GACGC 1 cut(s) 133
Hin1II CATG 7 cut(s) 272, 343, 374, 440, 504, 535, 590
HincII GTYRAC 1 cut(s) 231
HindII GTYRAC 1 cut(s) 231
HpaII CCGG 2 cut(s) 72, 277
HphI GGTGA 1 cut(s) 530
Hpy166II GTNNAC 1 cut(s) 231
Hpy188I TCNGA 4 cut(s) 94, 149, 239, 600
Hpy188III TCNNGA 1 cut(s) 352
Hpy8I GTNNAC 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 44, 369
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 3 cut(s) 268, 343, 563
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 38, 41, 94, 126, 587
HpyF3I CTNAG 3 cut(s) 212, 542, 582
Hsp92II CATG 7 cut(s) 272, 343, 374, 440, 504, 535, 590
Kzo9I GATC 1 cut(s) 149
LpnPI CCDG 8 cut(s) 7, 85, 224, 270, 290, 360, 469, 549
Lsp1109I GCAGC 4 cut(s) 19, 53, 116, 259
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 3 cut(s) 113, 422, 442
MalI GATC 1 cut(s) 151
MboI GATC 1 cut(s) 149
MboII GAAGA 1 cut(s) 206
MfeI CAATTG 1 cut(s) 77
MhlI GDGCHC 2 cut(s) 218, 322
MluCI AATT 7 cut(s) 77, 183, 380, 447, 489, 496, 537
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 5 cut(s) 173, 322, 358, 389, 587
Mph1103I ATGCAT 1 cut(s) 270
MseI TTAA 2 cut(s) 408, 492
MspI CCGG 2 cut(s) 72, 277
MspR9I CCNGG 1 cut(s) 277
MunI CAATTG 1 cut(s) 77
MwoI GCNNNNNNNGC 6 cut(s) 32, 38, 41, 94, 126, 587
NciI CCSGG 1 cut(s) 277
NdeI CATATG 1 cut(s) 556
NdeII GATC 1 cut(s) 149
NlaIII CATG 7 cut(s) 272, 343, 374, 440, 504, 535, 590
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 3 cut(s) 113, 422, 442
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 2 cut(s) 272, 590
PaeI GCATGC 2 cut(s) 272, 590
PaeR7I CTCGAG 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 483
PkrI GCNGC 6 cut(s) 34, 37, 40, 43, 131, 274
PsiI TTATAA 1 cut(s) 291
PspFI CCCAGC 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 1 cut(s) 235
PspXI VCTCGAGB 1 cut(s) 326
RsaI GTAC 3 cut(s) 203, 300, 458
RsaNI GTAC 3 cut(s) 202, 299, 457
Rsr2I CGGWCCG 1 cut(s) 235
RsrII CGGWCCG 1 cut(s) 235
SaqAI TTAA 2 cut(s) 408, 492
SatI GCNGC 6 cut(s) 33, 36, 39, 42, 130, 273
Sau3AI GATC 1 cut(s) 149
Sau96I GGNCC 1 cut(s) 235
ScrFI CCNGG 1 cut(s) 277
SduI GDGCHC 2 cut(s) 218, 322
SetI ASST 5 cut(s) 16, 157, 213, 400, 568
Sfr274I CTCGAG 1 cut(s) 326
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 2 cut(s) 272, 590
Sse9I AATT 7 cut(s) 77, 183, 380, 447, 489, 496, 537
SsiI CCGC 4 cut(s) 35, 38, 118, 420
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 275
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 3 cut(s) 327, 353, 363
TasI AATT 7 cut(s) 77, 183, 380, 447, 489, 496, 537
TauI GCSGC 2 cut(s) 38, 41
Tru1I TTAA 2 cut(s) 408, 492
Tru9I TTAA 2 cut(s) 408, 492
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 3 cut(s) 113, 422, 442
TseI GCWGC 4 cut(s) 32, 41, 129, 272
Tsp45I GTSAC 3 cut(s) 113, 422, 442
TspDTI ATGAA 1 cut(s) 489
TspRI CASTG 1 cut(s) 51
Van91I CCANNNNNTGG 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 235
XapI RAATTY 3 cut(s) 380, 496, 537
XceI RCATGY 2 cut(s) 272, 590
XhoI CTCGAG 1 cut(s) 326
XspI CTAG 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.