MD04G1021100.v1.1

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000430
Physical Location & Seq
Reverse (-)
2494579 .. 2496579
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1021100.v1.1.491

Sequence Viewer

Length: 1239 bp
ATGGATTTGTTTTCATGGATTTGGCGAATCGACATGGATGCACACACAAAGAAAACCGAACCAACCAACACTGACATAGAAGAGGATGATAAGAAACCCTACATTCTGCAATTGCCAGACCACATAACAGTGCAGATATTCTGCAAAGTCCCAATTAAAACCCTCATCCAATGCCAGTGTGTGTGCAAGTCTTGGCGCCATTCGCTCTCAGACCCCCAGTTTACCAAACACCTATTTTCACAAACACCCCCTTCATTTTTGTTCCATGGCTTAAGAGGCGGCCCCATAAACCCCAAATCGGAGGGATACTTATTGTTCGACCTTGATAAGGTCTCAAACCCAAATGACTTTGTACTGAAGCTTGCTAGAGATCCAAATGTCCACGCTCTTCGTGTGGGCATTTTGGGTTCTTGCAATGGTTTGCTCTGCATATATGAATTGAACCAACGCCATTTTTACATTTCCAATCCCATTACTAGCGAGTCTTTAGCGCTTCCAGTGATATCCTTGTCTCCCCATACTATCGCCTATTGTCAGCGTCGGAGAATTGATAATCCGTATTATTGTGGGTTTGGGTTTAGTCCCATCAGTAATGTCTACAAAGTTGTTGTGTTTATGTCTCCGAGTGATAAATCTAGTGAAGATGAAGTATTGGTTTTGACTGTTGGCTCCAGGATTTGGAGAAGCATTGGGGATGTGTCTGTGTTCCCTTCCTCTCGGTTTCAACCATATGGGGTTTATCTTAATGGATTCCTTCATTGGGTTGGTCAGTATGGGGATAGTTCGACTTTCATATGGGCCTTTGACGTTGAAAGGGAGCATTTCCAGCAGTTACCACTTCCTCCCTGTTATTTGGACTTAGACCAGCTGCCGAGCTGTTTGGATTTGCTAGAAACTCAATTTACCCTTGGAGTCTTGAATGGTTGCCTCTCTGTAACTGTTCGCTCTTGCTCGACTCGTAATATCAATGTCTGGATGATGAAGGATTATGGCATTAACGAGTCTTGGACCAAAGAGCTTGAAATTAGCAACGAAGCACTTGATTCTCCAACTTTTGCCCGAGTGTTGAAGTTTACAGAGGAGGGGAAAGTCTTGTTGTTAAATGGGTCTCACTTGCAGGCTTACACTCCTGGAACAAGGGGTGTTGTGATGGTTGAGGTTGATGGGATGCTATCATTGATCGATGAGTGTGTTCATATTCCAAGCTTTCTGCAGCTTAGAGATATCATCGCAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

413

Amino Acids

46.76

Weight (kDa)

5.64

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 35 - 79 1.2e-09 F-box-like
F-box PF00646 35 - 76 1.6e-09 F-box domain
FBA_1 PF07734 117 - 367 4.9e-20 F-box associated beta propeller domain
FBA_3 PF08268 132 - 368 2.8e-22 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000430)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g34920 FvH4_3g37340 FvH4_4g12931 FvH4_4g13090 FvH4_4g13100 FvH4_6g33904 FvH4_6g39874
malus_domestica MD04G1018800.v1.1 MD04G1020000.v1.1 MD04G1020500.v1.1 MD04G1021100.v1.1
prunus_persica Prupe.1G167600_v2.0.a1 Prupe.1G167800_v2.0.a1 Prupe.1G167900_v2.0.a1 Prupe.1G168000_v2.0.a1 Prupe.1G168100_v2.0.a1 Prupe.1G169600_v2.0.a1 Prupe.1G169800_v2.0.a1 Prupe.1G169900_v2.0.a1 Prupe.1G170000_v2.0.a1 Prupe.1G170100_v2.0.a1 Prupe.1G170100_v2.0.a1 Prupe.1G187600_v2.0.a1
pyrus_communis pycom04g01610 pycom04g01630 pycom04g01660
rosa_chinensis RchiOBHm_Chr3g0474061 RchiOBHm_Chr3g0474071 RchiOBHm_Chr3g0494781 RchiOBHm_Chr4g0412631 RchiOBHm_Chr4g0412831 RchiOBHm_Chr4g0412861
rosa_laevigata RLG00000008252 RLG00000008255 RLG00000008272 RLG00000023952 RLG00000026909 RLG00000026910
rosa_multiflora Rmu_co8499129.1_g000001 Rmu_sc0001755.1_g000010 Rmu_sc0001755.1_g000011 Rmu_sc0002068.1_g000006 Rmu_sc0002072.1_g000048 Rmu_sc0002878.1_g000004 Rmu_sc0003022.1_g000007 Rmu_sc0003961.1_g000002 Rmu_sc0010735.1_g000006 Rmu_sc0016141.1_g000002
rosa_roxburghii Rroxscaffold_3G00261940 Rroxscaffold_4G00285070 Rroxscaffold_5G00356800 Rroxscaffold_5G00357100 Rroxscaffold_5G00357120 Rroxscaffold_6G00407500
rosa_rugosa Rorug01G0370900 Rorug03G0138300 Rorug03G0138700 Rorug04G0117100 Rorug04G0117200 Rorug04G0117300 Rorug04G0117300 Rorug07G0011200
rosa_samantha Rh1AG379300 Rh1BG342700 Rh1BG342800 Rh1BG384500 Rh1BG384800 Rh1CG356400 Rh1CG398400 Rh1CG398800 Rh1DG374100 Rh1DG415800 Rh1DG416000 Rh3AG188500 Rh3BG217500 Rh3BG217700 Rh3CG213400 Rh3CG213600 Rh4AG174500 Rh4AG175800 Rh4AG175900 Rh4AG176000 Rh4BG176100 Rh4BG176400 Rh4CG185400 Rh4CG186900 Rh4CG187100 Rh4DG172900 Rh4DG173100 Rh7AG137700 Rh7BG138000 Rh7CG141600
rosa_wichuraiana Rw4G014620 Rw4G014750 Rw4G014760 Rw4G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 195
AccB7I CCANNNNNTGG 1 cut(s) 678
AccI GTMKAC 1 cut(s) 597
AciI CCGC 1 cut(s) 279
AclWI GGATC 1 cut(s) 365
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 196
AfaI GTAC 1 cut(s) 354
AfeI AGCGCT 1 cut(s) 492
AfiI CCNNNNNNNGG 4 cut(s) 298, 328, 678, 760
AflII CTTAAG 1 cut(s) 271
AgsI TTSAA 6 cut(s) 442, 725, 812, 919, 1022, 1069
AjnI CCWGG 2 cut(s) 671, 1129
AluBI AGCT 6 cut(s) 361, 868, 876, 1018, 1206, 1216
AluI AGCT 6 cut(s) 361, 868, 876, 1018, 1206, 1216
Alw26I GTCTC 4 cut(s) 337, 516, 624, 1113
AlwI GGATC 1 cut(s) 365
Ama87I CYCGRG 1 cut(s) 1059
Aor51HI AGCGCT 1 cut(s) 492
AoxI GGCC 2 cut(s) 280, 798
ApeKI GCWGC 2 cut(s) 868, 1213
AspLEI GCGC 2 cut(s) 198, 493
AspS9I GGNCC 3 cut(s) 281, 798, 1008
AvaI CYCGRG 1 cut(s) 1059
AvaII GGWCC 1 cut(s) 1008
BanI GGYRCC 1 cut(s) 195
BbvI GCAGC 2 cut(s) 855, 1225
BccI CCATC 3 cut(s) 593, 1144, 1157
BcgI CGANNNNNNTGC 2 cut(s) 20, 54
BciT130I CCWGG 2 cut(s) 673, 1131
BciVI GTATCC 1 cut(s) 299
BcoDI GTCTC 4 cut(s) 337, 516, 624, 1113
BfaI CTAG 5 cut(s) 366, 477, 636, 890, 1237
BfmI CTRYAG 1 cut(s) 1211
BfoI RGCGCY 2 cut(s) 199, 494
BfrI CTTAAG 1 cut(s) 271
BfuI GTATCC 1 cut(s) 299
BisI GCNGC 3 cut(s) 280, 869, 1214
BlsI GCNGC 3 cut(s) 281, 870, 1215
Bme1390I CCNGG 2 cut(s) 673, 1131
Bme18I GGWCC 1 cut(s) 1008
BmeT110I CYCGRG 1 cut(s) 1059
BmgT120I GGNCC 3 cut(s) 281, 798, 1008
BmiI GGNNCC 3 cut(s) 197, 283, 670
BmrFI CCNGG 2 cut(s) 673, 1131
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 2 cut(s) 28, 1158
BmuI ACTGGG 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 655
Bsa29I ATCGAT 1 cut(s) 1182
BsaHI GRCGYC 1 cut(s) 196
BsaI GGTCTC 2 cut(s) 337, 1113
BsaJI CCNNGG 2 cut(s) 265, 907
Bsc4I CCNNNNNNNGG 4 cut(s) 298, 328, 678, 760
Bse1I ACTGG 3 cut(s) 175, 217, 497
Bse3DI GCAATG 1 cut(s) 421
BseBI CCWGG 2 cut(s) 673, 1131
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 2 cut(s) 265, 907
BseGI GGATG 6 cut(s) 43, 91, 165, 700, 981, 1173
BseLI CCNNNNNNNGG 4 cut(s) 298, 328, 678, 760
BseMI GCAATG 1 cut(s) 421
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 3 cut(s) 175, 217, 497
BseRI GAGGAG 1 cut(s) 1094
BseXI GCAGC 2 cut(s) 855, 1225
BsgI GTGCAG 1 cut(s) 152
BshFI GGCC 2 cut(s) 282, 800
BshNI GGYRCC 1 cut(s) 195
BshVI ATCGAT 1 cut(s) 1182
BsiHKCI CYCGRG 1 cut(s) 1059
BslFI GGGAC 2 cut(s) 134, 567
BslI CCNNNNNNNGG 4 cut(s) 298, 328, 678, 760
BsmAI GTCTC 4 cut(s) 337, 516, 624, 1113
BsmFI GGGAC 2 cut(s) 134, 567
BsnI GGCC 2 cut(s) 282, 800
Bso31I GGTCTC 2 cut(s) 337, 1113
BsoBI CYCGRG 1 cut(s) 1059
Bsp143I GATC 2 cut(s) 370, 1179
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 1 cut(s) 279
BspANI GGCC 2 cut(s) 282, 800
BspCNI CTCAG 1 cut(s) 221
BspDI ATCGAT 1 cut(s) 1182
BspLI GGNNCC 3 cut(s) 197, 283, 670
BspMAI CTGCAG 1 cut(s) 1215
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 195
BspTI CTTAAG 1 cut(s) 271
BspTNI GGTCTC 2 cut(s) 337, 1113
BsrDI GCAATG 1 cut(s) 421
BsrI ACTGG 3 cut(s) 175, 217, 497
BssECI CCNNGG 2 cut(s) 265, 907
BssMI GATC 2 cut(s) 370, 1179
BssNI GRCGYC 1 cut(s) 196
BssT1I CCWWGG 2 cut(s) 265, 907
Bst2UI CCWGG 2 cut(s) 673, 1131
Bst4CI ACNGT 3 cut(s) 130, 664, 940
Bst6I CTCTTC 2 cut(s) 75, 393
BstACI GRCGYC 1 cut(s) 196
BstAFI CTTAAG 1 cut(s) 271
BstC8I GCNNGC 3 cut(s) 363, 1119, 1234
BstDEI CTNAG 3 cut(s) 208, 859, 1217
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 6 cut(s) 43, 91, 165, 700, 981, 1173
BstH2I RGCGCY 2 cut(s) 199, 494
BstHHI GCGC 2 cut(s) 198, 493
BstKTI GATC 2 cut(s) 373, 1182
BstMAI GTCTC 4 cut(s) 337, 516, 624, 1113
BstMBI GATC 2 cut(s) 370, 1179
BstMWI GCNNNNNNNGC 3 cut(s) 202, 276, 826
BstNI CCWGG 2 cut(s) 673, 1131
BstSCI CCNGG 2 cut(s) 671, 1129
BstSFI CTRYAG 1 cut(s) 1211
BstV1I GCAGC 2 cut(s) 855, 1225
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
Bsu15I ATCGAT 1 cut(s) 1182
BsuI GTATCC 1 cut(s) 299
BsuRI GGCC 2 cut(s) 282, 800
BsuTUI ATCGAT 1 cut(s) 1182
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 1213
BtsCI GGATG 6 cut(s) 43, 91, 165, 700, 981, 1173
BtsIMutI CAGTG 4 cut(s) 69, 135, 182, 504
Cac8I GCNNGC 3 cut(s) 363, 1119, 1234
CfoI GCGC 2 cut(s) 198, 493
Cfr13I GGNCC 3 cut(s) 281, 798, 1008
ClaI ATCGAT 1 cut(s) 1182
CseI GACGC 1 cut(s) 527
Csp6I GTAC 1 cut(s) 353
CviAII CATG 3 cut(s) 15, 34, 266
CviQI GTAC 1 cut(s) 353
DdeI CTNAG 3 cut(s) 208, 859, 1217
DinI GGCGCC 1 cut(s) 197
DpnI GATC 2 cut(s) 372, 1181
DpnII GATC 2 cut(s) 370, 1179
Eam1104I CTCTTC 2 cut(s) 75, 393
EarI CTCTTC 2 cut(s) 75, 393
Eco130I CCWWGG 2 cut(s) 265, 907
Eco31I GGTCTC 2 cut(s) 337, 1113
Eco32I GATATC 2 cut(s) 504, 1225
Eco47I GGWCC 1 cut(s) 1008
Eco47III AGCGCT 1 cut(s) 492
Eco57I CTGAAG 1 cut(s) 377
Eco88I CYCGRG 1 cut(s) 1059
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoRII CCWGG 2 cut(s) 671, 1129
EcoRV GATATC 2 cut(s) 504, 1225
EcoT14I CCWWGG 2 cut(s) 265, 907
EgeI GGCGCC 1 cut(s) 197
EheI GGCGCC 1 cut(s) 197
ErhI CCWWGG 2 cut(s) 265, 907
FaeI CATG 3 cut(s) 18, 37, 269
FaqI GGGAC 2 cut(s) 134, 567
FatI CATG 3 cut(s) 14, 33, 265
FauNDI CATATG 2 cut(s) 730, 794
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 3 cut(s) 280, 869, 1214
FokI GGATG 6 cut(s) 50, 98, 152, 707, 988, 1180
Fsp4HI GCNGC 3 cut(s) 280, 869, 1214
FspBI CTAG 5 cut(s) 366, 477, 636, 890, 1237
GlaI GCGC 2 cut(s) 197, 492
GluI GCNGC 3 cut(s) 280, 869, 1214
GsuI CTGGAG 1 cut(s) 655
HaeII RGCGCY 2 cut(s) 199, 494
HaeIII GGCC 2 cut(s) 282, 800
HgaI GACGC 1 cut(s) 527
HhaI GCGC 2 cut(s) 198, 493
Hin1I GRCGYC 1 cut(s) 196
Hin1II CATG 3 cut(s) 18, 37, 269
Hin6I GCGC 2 cut(s) 196, 491
HinP1I GCGC 2 cut(s) 196, 491
HindIII AAGCTT 2 cut(s) 359, 1204
HinfI GANTC 7 cut(s) 27, 482, 750, 912, 955, 1001, 1043
Hpy166II GTNNAC 4 cut(s) 222, 382, 598, 1074
Hpy188I TCNGA 4 cut(s) 211, 301, 543, 624
Hpy188III TCNNGA 2 cut(s) 916, 973
Hpy8I GTNNAC 4 cut(s) 222, 382, 598, 1074
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 4 cut(s) 261, 720, 764, 976
HpyCH4III ACNGT 3 cut(s) 130, 664, 940
HpyCH4IV ACGT 1 cut(s) 807
HpyCH4V TGCA 9 cut(s) 41, 109, 133, 144, 186, 414, 429, 1117, 1213
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 276, 826
HpyF3I CTNAG 3 cut(s) 208, 859, 1217
HpySE526I ACGT 1 cut(s) 807
Hsp92I GRCGYC 1 cut(s) 196
Hsp92II CATG 3 cut(s) 18, 37, 269
HspAI GCGC 2 cut(s) 196, 491
KasI GGCGCC 1 cut(s) 195
Kzo9I GATC 2 cut(s) 370, 1179
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 674, 817
Lsp1109I GCAGC 2 cut(s) 855, 1225
LweI GCATC 2 cut(s) 28, 1158
MaeI CTAG 5 cut(s) 366, 477, 636, 890, 1237
MaeII ACGT 1 cut(s) 807
MaeIII GTNAC 2 cut(s) 831, 934
MalI GATC 2 cut(s) 372, 1181
MboI GATC 2 cut(s) 370, 1179
MboII GAAGA 3 cut(s) 92, 380, 653
MfeI CAATTG 1 cut(s) 110
MflI RGATCY 1 cut(s) 370
MluCI AATT 6 cut(s) 110, 153, 437, 546, 899, 1023
Mly113I GGCGCC 1 cut(s) 196
MlyI GAGTC 4 cut(s) 491, 921, 949, 1010
MmeI TCCRAC 2 cut(s) 521, 1073
MseI TTAA 5 cut(s) 156, 272, 744, 996, 1100
MslI CAYNNNNRTG 1 cut(s) 128
MspA1I CMGCKG 1 cut(s) 868
MspCI CTTAAG 1 cut(s) 271
MspR9I CCNGG 2 cut(s) 673, 1131
MunI CAATTG 1 cut(s) 110
MvaI CCWGG 2 cut(s) 673, 1131
MwoI GCNNNNNNNGC 3 cut(s) 202, 276, 826
NarI GGCGCC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 265
NdeI CATATG 2 cut(s) 730, 794
NdeII GATC 2 cut(s) 370, 1179
NlaIII CATG 3 cut(s) 18, 37, 269
NlaIV GGNNCC 3 cut(s) 197, 283, 670
NmeAIII GCCGAG 1 cut(s) 897
PciSI GCTCTTC 1 cut(s) 393
PfeI GAWTC 3 cut(s) 27, 750, 1043
PflMI CCANNNNNTGG 1 cut(s) 678
PfoI TCCNGGA 2 cut(s) 671, 1129
PkrI GCNGC 3 cut(s) 281, 870, 1215
PleI GAGTC 4 cut(s) 490, 920, 949, 1009
PluTI GGCGCC 1 cut(s) 199
PpsI GAGTC 4 cut(s) 490, 920, 949, 1009
Psp6I CCWGG 2 cut(s) 671, 1129
PspGI CCWGG 2 cut(s) 671, 1129
PspN4I GGNNCC 3 cut(s) 197, 283, 670
PspPI GGNCC 3 cut(s) 281, 798, 1008
PstI CTGCAG 1 cut(s) 1215
PsuI RGATCY 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 1 cut(s) 354
RsaNI GTAC 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 128
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 5 cut(s) 156, 272, 744, 996, 1100
SatI GCNGC 3 cut(s) 280, 869, 1214
Sau3AI GATC 2 cut(s) 370, 1179
Sau96I GGNCC 3 cut(s) 281, 798, 1008
SchI GAGTC 4 cut(s) 491, 921, 949, 1010
ScrFI CCNGG 2 cut(s) 673, 1131
SfaNI GCATC 2 cut(s) 28, 1158
SfcI CTRYAG 1 cut(s) 1211
SfoI GGCGCC 1 cut(s) 197
SinI GGWCC 1 cut(s) 1008
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 6 cut(s) 110, 153, 437, 546, 899, 1023
SsiI CCGC 1 cut(s) 279
SspDI GGCGCC 1 cut(s) 195
SspMI CTAG 5 cut(s) 366, 477, 636, 890, 1237
StyD4I CCNGG 2 cut(s) 671, 1129
StyI CCWWGG 2 cut(s) 265, 907
TaaI ACNGT 3 cut(s) 130, 664, 940
TaiI ACGT 1 cut(s) 810
TaqI TCGA 5 cut(s) 30, 318, 785, 953, 1182
TasI AATT 6 cut(s) 110, 153, 437, 546, 899, 1023
TatI WGTACW 1 cut(s) 352
TauI GCSGC 1 cut(s) 282
TfiI GAWTC 3 cut(s) 27, 750, 1043
Tru1I TTAA 5 cut(s) 156, 272, 744, 996, 1100
Tru9I TTAA 5 cut(s) 156, 272, 744, 996, 1100
TscAI CASTG 4 cut(s) 76, 135, 182, 504
TseI GCWGC 2 cut(s) 868, 1213
TspDTI ATGAA 7 cut(s) 243, 450, 660, 746, 781, 995, 1184
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 4 cut(s) 76, 135, 182, 504
Van91I CCANNNNNTGG 1 cut(s) 678
Vha464I CTTAAG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 1008
XagI CCTNNNNNAGG 1 cut(s) 326
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 5 cut(s) 366, 477, 636, 890, 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.