pycom04g01630

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000430
Physical Location & Seq
Reverse (-)
1569430 .. 1572304
2875 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01630.2

Sequence Viewer

Length: 1200 bp
ATGACATCTCCAGCCATGAAGAAAATCAAACCCAAGCACACCAGCCCGCCAACCAACTCCGACGCCGAACCGCCAAAGCAAAAAGATGAACAGGAAGAAGGAAACGAGAAGAAGCCCTACATTCTCCAACTCCCAAACCACATAACGGTCGAGATCTTCGGCAAAATCCCAATCAAAGCTCTGATACAATGCCGCTGCGTGTGCAAGTCATGGCGCCGATCGCTCTCGGACCCCCAATTCACAAAGTACCTACTTTCACAGACACCCGATTGCCTTTTGCTCCAAAACAGCAGCACCCGAAACCCCAACACCGCCGGCCTCTTCTTGGTCGACCTCGACAAACCCTCAGGCAAGAACGACGTCGTAATCAGGCTTCCCAAAGACCCCAATGTCCCGAATTTCGGTGTGCAAATTGTGGGTTCCTGCAATGGCTTCCTCTGCGTGTACGATAGGCTTAACAGCGGTCGGTTTTACATCTCCAATCCCGTTATTGGTGAGTCCATAACTCTTCCAAGATTTCCCAAGGATATTGCTTTTCCGTTTCTTTATGGGTTTGGGTTTAGTCCTGTCGATGGTGTCTATAAATTAGTTGTGGTTGCATCTACGAGTAACGGATTGAAATCGAATGAATGGGAGGTAATGGTTTTGACTGTTGGCACTGGAAGTTGGAGAAATGTTGGGAATTCTGTGTACCCTTTTGGGTACCAATCGTATGGGGTTTATCATGACGGTTTTCTTCATTGGATTGCTCGTAGCGACAATTCTGTATTGATTTGTGCCTTTGATGTTGGAAGTGAGTGTTTCCAAGAGTTGCCATTACCGCCTTGTTCTTTGGGAAAGCGTCTTGTTAGCCTCGCTGCCCTGAGAGGCTGCCTCTCCGTATACGTTCTCTCGATGAGTGATATCAATGTTTGGGTGATGAAGGATTATGGCGTTAAGGAATCGTGGGCCGAGGAGCTTGTCATGCAGCAAGTGATTGGGTACGGAACTAGTTTTTCTGCTACTCAATTGTTGAAATTTACAAAGAAGGGCCAAGTGGTGTTTTCACTCAAGTATAAACTGAGGGTTTATACTCCTGGAAAAAAGGGCTCTGCTGCGGTTGAAGTTGATGGAATACCATCAATGGTTGAAGCATTTGTCCATATTCCAAGCGTTGTTTCTCTTAAGGATGCCATCATGGATTTGAGCTCAGAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1 FBA_3

Protein Analysis

400

Amino Acids

44.18

Weight (kDa)

8.66

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000430)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g34920 FvH4_3g37340 FvH4_4g12931 FvH4_4g13090 FvH4_4g13100 FvH4_6g33904 FvH4_6g39874
malus_domestica MD04G1018800.v1.1 MD04G1020000.v1.1 MD04G1020500.v1.1 MD04G1021100.v1.1
prunus_persica Prupe.1G167600_v2.0.a1 Prupe.1G167800_v2.0.a1 Prupe.1G167900_v2.0.a1 Prupe.1G168000_v2.0.a1 Prupe.1G168100_v2.0.a1 Prupe.1G169600_v2.0.a1 Prupe.1G169800_v2.0.a1 Prupe.1G169900_v2.0.a1 Prupe.1G170000_v2.0.a1 Prupe.1G170100_v2.0.a1 Prupe.1G170100_v2.0.a1 Prupe.1G187600_v2.0.a1
pyrus_communis pycom04g01610 pycom04g01630 pycom04g01660
rosa_chinensis RchiOBHm_Chr3g0474061 RchiOBHm_Chr3g0474071 RchiOBHm_Chr3g0494781 RchiOBHm_Chr4g0412631 RchiOBHm_Chr4g0412831 RchiOBHm_Chr4g0412861
rosa_laevigata RLG00000008252 RLG00000008255 RLG00000008272 RLG00000023952 RLG00000026909 RLG00000026910
rosa_multiflora Rmu_co8499129.1_g000001 Rmu_sc0001755.1_g000010 Rmu_sc0001755.1_g000011 Rmu_sc0002068.1_g000006 Rmu_sc0002072.1_g000048 Rmu_sc0002878.1_g000004 Rmu_sc0003022.1_g000007 Rmu_sc0003961.1_g000002 Rmu_sc0010735.1_g000006 Rmu_sc0016141.1_g000002
rosa_roxburghii Rroxscaffold_3G00261940 Rroxscaffold_4G00285070 Rroxscaffold_5G00356800 Rroxscaffold_5G00357100 Rroxscaffold_5G00357120 Rroxscaffold_6G00407500
rosa_rugosa Rorug01G0370900 Rorug03G0138300 Rorug03G0138700 Rorug04G0117100 Rorug04G0117200 Rorug04G0117300 Rorug04G0117300 Rorug07G0011200
rosa_samantha Rh1AG379300 Rh1BG342700 Rh1BG342800 Rh1BG384500 Rh1BG384800 Rh1CG356400 Rh1CG398400 Rh1CG398800 Rh1DG374100 Rh1DG415800 Rh1DG416000 Rh3AG188500 Rh3BG217500 Rh3BG217700 Rh3CG213400 Rh3CG213600 Rh4AG174500 Rh4AG175800 Rh4AG175900 Rh4AG176000 Rh4BG176100 Rh4BG176400 Rh4CG185400 Rh4CG186900 Rh4CG187100 Rh4DG172900 Rh4DG173100 Rh7AG137700 Rh7BG138000 Rh7CG141600
rosa_wichuraiana Rw4G014620 Rw4G014750 Rw4G014760 Rw4G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 389
AatII GACGTC 1 cut(s) 363
Acc65I GGTACC 1 cut(s) 702
AccB1I GGYRCC 2 cut(s) 213, 702
AccI GTMKAC 2 cut(s) 330, 882
AciI CCGC 7 cut(s) 47, 71, 193, 312, 462, 821, 1097
AcsI RAATTY 3 cut(s) 397, 682, 1016
AcyI GRCGYC 3 cut(s) 63, 214, 360
AfaI GTAC 5 cut(s) 248, 446, 692, 704, 983
AfiI CCNNNNNNNGG 5 cut(s) 145, 325, 401, 491, 572
AflII CTTAAG 1 cut(s) 1163
AgsI TTSAA 4 cut(s) 619, 1015, 1103, 1130
AhlI ACTAGT 1 cut(s) 989
AjnI CCWGG 1 cut(s) 1075
AjuI GAANNNNNNNTTGG 2 cut(s) 1141, 1173
AluBI AGCT 3 cut(s) 179, 958, 1188
AluI AGCT 3 cut(s) 179, 958, 1188
Alw21I GWGCWC 1 cut(s) 1190
AoxI GGCC 3 cut(s) 316, 948, 1030
ApeKI GCWGC 6 cut(s) 195, 291, 857, 870, 967, 1094
ApoI RAATTY 3 cut(s) 397, 682, 1016
Asp718I GGTACC 1 cut(s) 702
AspLEI GCGC 1 cut(s) 216
AspS9I GGNCC 3 cut(s) 229, 948, 1030
AsuHPI GGTGA 2 cut(s) 506, 928
AvaII GGWCC 1 cut(s) 229
AxyI CCTNAGG 1 cut(s) 346
BanI GGYRCC 2 cut(s) 213, 702
BanII GRGCYC 2 cut(s) 1091, 1190
BarI GAAGNNNNNNTAC 4 cut(s) 101, 133, 357, 389
Bbv12I GWGCWC 1 cut(s) 1190
BbvI GCAGC 6 cut(s) 182, 303, 844, 857, 979, 1081
BccI CCATC 4 cut(s) 566, 1103, 1126, 1181
BciT130I CCWGG 1 cut(s) 1077
BcuI ACTAGT 1 cut(s) 989
BfaI CTAG 1 cut(s) 990
BfoI RGCGCY 1 cut(s) 217
BfrI CTTAAG 1 cut(s) 1163
BglII AGATCT 1 cut(s) 153
BisI GCNGC 7 cut(s) 193, 196, 292, 858, 871, 968, 1095
BlsI GCNGC 7 cut(s) 194, 197, 293, 859, 872, 969, 1096
Bme1390I CCNGG 1 cut(s) 1077
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 229, 948, 1030
BmiI GGNNCC 4 cut(s) 215, 231, 421, 704
BmrFI CCNGG 1 cut(s) 1077
BmsI GCATC 2 cut(s) 608, 1159
BplI GAGNNNNNCTC 2 cut(s) 858, 890
BpuEI CTTGAG 1 cut(s) 1034
BsaBI GATNNNNATC 1 cut(s) 619
BsaHI GRCGYC 3 cut(s) 63, 214, 360
BsaJI CCNNGG 2 cut(s) 522, 951
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 325, 401, 491, 572
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 664
Bse21I CCTNAGG 1 cut(s) 346
Bse3DI GCAATG 1 cut(s) 433
Bse8I GATNNNNATC 1 cut(s) 619
BseBI CCWGG 1 cut(s) 1077
BseDI CCNNGG 2 cut(s) 522, 951
BseGI GGATG 1 cut(s) 1174
BseJI GATNNNNATC 1 cut(s) 619
BseLI CCNNNNNNNGG 5 cut(s) 145, 325, 401, 491, 572
BseMI GCAATG 1 cut(s) 433
BseMII CTCAG 3 cut(s) 360, 854, 1052
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 1 cut(s) 968
BseXI GCAGC 6 cut(s) 182, 303, 844, 857, 979, 1081
Bsh1285I CGRYCG 3 cut(s) 150, 221, 466
BshFI GGCC 3 cut(s) 318, 950, 1032
BshNI GGYRCC 2 cut(s) 213, 702
BsiEI CGRYCG 3 cut(s) 150, 221, 466
BsiHKAI GWGCWC 1 cut(s) 1190
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 5 cut(s) 145, 325, 401, 491, 572
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 3 cut(s) 318, 950, 1032
Bsp1286I GDGCHC 2 cut(s) 1091, 1190
Bsp143I GATC 2 cut(s) 153, 218
BspACI CCGC 7 cut(s) 47, 71, 193, 312, 462, 821, 1097
BspANI GGCC 3 cut(s) 318, 950, 1032
BspCNI CTCAG 3 cut(s) 359, 855, 1053
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 4 cut(s) 215, 231, 421, 704
BspT107I GGYRCC 2 cut(s) 213, 702
BspTI CTTAAG 1 cut(s) 1163
BsrDI GCAATG 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 522, 951
BssMI GATC 2 cut(s) 153, 218
BssNAI GTATAC 1 cut(s) 883
BssNI GRCGYC 3 cut(s) 63, 214, 360
BssT1I CCWWGG 1 cut(s) 522
Bst1107I GTATAC 1 cut(s) 883
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 3 cut(s) 148, 652, 731
Bst6I CTCTTC 2 cut(s) 326, 513
BstACI GRCGYC 3 cut(s) 63, 214, 360
BstAFI CTTAAG 1 cut(s) 1163
BstC8I GCNNGC 2 cut(s) 47, 316
BstDEI CTNAG 4 cut(s) 346, 863, 1061, 1189
BstF5I GGATG 1 cut(s) 1174
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 2 cut(s) 156, 221
BstMBI GATC 2 cut(s) 153, 218
BstMCI CGRYCG 3 cut(s) 150, 221, 466
BstMWI GCNNNNNNNGC 5 cut(s) 201, 220, 438, 820, 964
BstNI CCWGG 1 cut(s) 1077
BstSCI CCNGG 1 cut(s) 1075
BstV1I GCAGC 6 cut(s) 182, 303, 844, 857, 979, 1081
BstX2I RGATCY 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 1 cut(s) 153
BstZ17I GTATAC 1 cut(s) 883
Bsu36I CCTNAGG 1 cut(s) 346
BsuRI GGCC 3 cut(s) 318, 950, 1032
BtsCI GGATG 1 cut(s) 1174
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 47, 316
CciI TCATGA 1 cut(s) 724
CfoI GCGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 3 cut(s) 229, 948, 1030
CseI GACGC 2 cut(s) 71, 830
Csp6I GTAC 5 cut(s) 247, 445, 691, 703, 982
CviAII CATG 5 cut(s) 16, 210, 725, 964, 1177
CviQI GTAC 5 cut(s) 247, 445, 691, 703, 982
DdeI CTNAG 4 cut(s) 346, 863, 1061, 1189
DinI GGCGCC 1 cut(s) 215
DpnI GATC 2 cut(s) 155, 220
DpnII GATC 2 cut(s) 153, 218
DrdI GACNNNNNNGTC 1 cut(s) 389
DseDI GACNNNNNNGTC 1 cut(s) 389
Eam1104I CTCTTC 2 cut(s) 326, 513
EarI CTCTTC 2 cut(s) 326, 513
Ecl136II GAGCTC 1 cut(s) 1188
Eco130I CCWWGG 1 cut(s) 522
Eco24I GRGCYC 2 cut(s) 1091, 1190
Eco32I GATATC 1 cut(s) 904
Eco47I GGWCC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 1188
Eco81I CCTNAGG 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 1188
EcoRI GAATTC 1 cut(s) 682
EcoRII CCWGG 1 cut(s) 1075
EcoRV GATATC 1 cut(s) 904
EcoT14I CCWWGG 1 cut(s) 522
EcoT38I GRGCYC 2 cut(s) 1091, 1190
EgeI GGCGCC 1 cut(s) 215
EheI GGCGCC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 5 cut(s) 19, 213, 728, 967, 1180
FaqI GGGAC 1 cut(s) 377
FatI CATG 5 cut(s) 15, 209, 724, 963, 1176
FauI CCCGC 1 cut(s) 54
FblI GTMKAC 2 cut(s) 330, 882
Fnu4HI GCNGC 7 cut(s) 193, 196, 292, 858, 871, 968, 1095
FokI GGATG 1 cut(s) 1181
FriOI GRGCYC 2 cut(s) 1091, 1190
Fsp4HI GCNGC 7 cut(s) 193, 196, 292, 858, 871, 968, 1095
FspBI CTAG 1 cut(s) 990
GlaI GCGC 1 cut(s) 215
GluI GCNGC 7 cut(s) 193, 196, 292, 858, 871, 968, 1095
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 3 cut(s) 318, 950, 1032
HapII CCGG 1 cut(s) 315
HgaI GACGC 2 cut(s) 71, 830
HhaI GCGC 1 cut(s) 216
Hin1I GRCGYC 3 cut(s) 63, 214, 360
Hin1II CATG 5 cut(s) 19, 213, 728, 967, 1180
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 2 cut(s) 497, 941
HpaII CCGG 1 cut(s) 315
HphI GGTGA 2 cut(s) 506, 928
Hpy166II GTNNAC 4 cut(s) 331, 445, 691, 883
Hpy188I TCNGA 4 cut(s) 61, 183, 229, 1192
Hpy188III TCNNGA 4 cut(s) 151, 394, 725, 892
Hpy8I GTNNAC 4 cut(s) 331, 445, 691, 883
Hpy99I CGWCG 3 cut(s) 65, 362, 365
HpyAV CCTTC 3 cut(s) 92, 916, 1021
HpyCH4III ACNGT 3 cut(s) 148, 652, 731
HpyCH4IV ACGT 2 cut(s) 360, 885
HpyCH4V TGCA 5 cut(s) 204, 409, 426, 599, 967
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 220, 438, 820, 964
HpyF3I CTNAG 4 cut(s) 346, 863, 1061, 1189
HpySE526I ACGT 2 cut(s) 360, 885
Hsp92I GRCGYC 3 cut(s) 63, 214, 360
Hsp92II CATG 5 cut(s) 19, 213, 728, 967, 1180
HspAI GCGC 1 cut(s) 214
KasI GGCGCC 1 cut(s) 213
KpnI GGTACC 1 cut(s) 706
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 153, 218
LmnI GCTCC 2 cut(s) 285, 955
Lsp1109I GCAGC 6 cut(s) 182, 303, 844, 857, 979, 1081
LweI GCATC 2 cut(s) 608, 1159
MaeI CTAG 1 cut(s) 990
MaeII ACGT 2 cut(s) 360, 885
MaeIII GTNAC 1 cut(s) 608
MalI GATC 2 cut(s) 155, 220
MboI GATC 2 cut(s) 153, 218
MboII GAAGA 7 cut(s) 31, 107, 121, 148, 313, 500, 728
MfeI CAATTG 1 cut(s) 1007
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 2 cut(s) 1091, 1190
MluCI AATT 8 cut(s) 236, 397, 411, 584, 682, 760, 1007, 1016
Mly113I GGCGCC 1 cut(s) 214
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 4 cut(s) 84, 151, 647, 769
MroNI GCCGGC 1 cut(s) 314
MseI TTAA 3 cut(s) 456, 936, 1164
MspA1I CMGCKG 2 cut(s) 195, 462
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 1077
MunI CAATTG 1 cut(s) 1007
MvaI CCWGG 1 cut(s) 1077
MwoI GCNNNNNNNGC 5 cut(s) 201, 220, 438, 820, 964
NaeI GCCGGC 1 cut(s) 316
NarI GGCGCC 1 cut(s) 214
NdeII GATC 2 cut(s) 153, 218
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 5 cut(s) 19, 213, 728, 967, 1180
NlaIV GGNNCC 4 cut(s) 215, 231, 421, 704
NmeAIII GCCGAG 1 cut(s) 976
PagI TCATGA 1 cut(s) 724
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 1 cut(s) 941
PfoI TCCNGGA 1 cut(s) 1075
PkrI GCNGC 7 cut(s) 194, 197, 293, 859, 872, 969, 1096
Ple19I CGATCG 1 cut(s) 221
PleI GAGTC 1 cut(s) 505
PluTI GGCGCC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 505
Psp124BI GAGCTC 1 cut(s) 1190
Psp6I CCWGG 1 cut(s) 1075
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 4 cut(s) 215, 231, 421, 704
PspPI GGNCC 3 cut(s) 229, 948, 1030
PsuI RGATCY 1 cut(s) 153
PvuI CGATCG 1 cut(s) 221
RsaI GTAC 5 cut(s) 248, 446, 692, 704, 983
RsaNI GTAC 5 cut(s) 247, 445, 691, 703, 982
SacI GAGCTC 1 cut(s) 1190
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 3 cut(s) 456, 936, 1164
SatI GCNGC 7 cut(s) 193, 196, 292, 858, 871, 968, 1095
Sau3AI GATC 2 cut(s) 153, 218
Sau96I GGNCC 3 cut(s) 229, 948, 1030
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 1077
SduI GDGCHC 2 cut(s) 1091, 1190
SetI ASST 8 cut(s) 181, 252, 336, 363, 639, 888, 960, 1190
SfaNI GCATC 2 cut(s) 608, 1159
SfoI GGCGCC 1 cut(s) 215
SinI GGWCC 1 cut(s) 229
SmlI CTYRAG 2 cut(s) 1049, 1163
SmoI CTYRAG 2 cut(s) 1049, 1163
SpeI ACTAGT 1 cut(s) 989
Sse9I AATT 8 cut(s) 236, 397, 411, 584, 682, 760, 1007, 1016
SsiI CCGC 7 cut(s) 47, 71, 193, 312, 462, 821, 1097
SspDI GGCGCC 1 cut(s) 213
SspMI CTAG 1 cut(s) 990
SstI GAGCTC 1 cut(s) 1190
StyD4I CCNGG 1 cut(s) 1075
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 3 cut(s) 148, 652, 731
TaiI ACGT 2 cut(s) 363, 888
TaqI TCGA 6 cut(s) 150, 330, 336, 570, 623, 893
TasI AATT 8 cut(s) 236, 397, 411, 584, 682, 760, 1007, 1016
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 1 cut(s) 941
Tru1I TTAA 3 cut(s) 456, 936, 1164
Tru9I TTAA 3 cut(s) 456, 936, 1164
TscAI CASTG 1 cut(s) 664
TseI GCWGC 6 cut(s) 195, 291, 857, 870, 967, 1094
TspDTI ATGAA 5 cut(s) 32, 102, 642, 728, 935
TspGWI ACGGA 4 cut(s) 528, 627, 868, 999
TspRI CASTG 1 cut(s) 664
Vha464I CTTAAG 1 cut(s) 1163
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 3 cut(s) 397, 682, 1016
XmiI GTMKAC 2 cut(s) 330, 882
XspI CTAG 1 cut(s) 990
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.