RchiOBHm_Chr3g0474061

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000430
Physical Location & Seq
Reverse (-)
19789492 .. 19790724
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43971

Sequence Viewer

Length: 1233 bp
ATGAAAATCAAAACAACCAACACTGAGAGAGATCAAGAAGTAGAAGACAAAAGAGATGATGAGAAACCCTGCATTATTCTACAATTGCCCGACCACATAATCCTGGAGATCTTCTGTAGAATCCCAATAAACACGGTCATCCAATGCAAGTATGTATGCAAGTCTTGGCGCCGTTCTCTCACAGACCAAGAGTTCTCAAAATCCCTATTTGCACGTACTCCGGCGTGCTTGTTTCTCCAAGACTATAGTAGGACCCCATGCCGGAGGCAAAACACCATACGTCATCGTATACCCCCGAATAAGCACATCTTGGCCAACTTGCAAAGCGTCTCGAGCCCAAACAACGTGGTCTTGAAGCTTTCAGATCCCAATGCCTTATACAACAGGCTCCATTCAAGTGTTACAGGTATTATGGGATCCTGCAATGGCTTCCTCTGCTTATTCCGCTATAATTGTAAAATTGGTGCTTTCATTTTTAACATATCCAATCCCATTACCGGCGAGTCTGTACCTCTTCCAGTCAATAAAGAAATAGGCCGTCCGGCTCACTTTGGGTTTGGGTTTAGTCCCATAAGTGATGTCTATAAGGTGGTTGTGTTTACATCCAATGAAGATAATGAACATCGCTTTCACAAAAGAAAATTGGACGTGATGGTTTTGACTGTTGGCTCTGGGATTTGGAGAAGAATTGGCAAAGTATCCAATGTCATGCATGGAACACGAGAAGGGGTCTTTCATAATGGATTTCTTCACTGGGTTTGCCACTGTAGAAAAGGTTGTCACTTTTTCATTCGTGCATTTGATGTTGAAAGCGAGCGTTTCAAGGATTTACCAATGCCCCCTTATTATTTCCGTCCCGATTCTATACCTCAAATGCGAGTCTTAGGAGGTTCACTTTCTGTAAGTGATGGCCGCACGTTTTGGGTAATGAAAGAGTACGGCGTCAAGAGGTCTTGGACCAAGGAGCTTGTAATTGGACATGATACAATTGATCCTAATGACGCATATGGGTCCTATATACGATATGTTTCAGTTTTGAAATTTACAGAGGGGAAAGTTTTGTTGTTAGCAAACACTAATTTGTGCCTCTATACTCCTGAAACAAGGACCCTTGAGAGGGTTGAAATTGATGGGATGCCACCAGGGGGCTTCCATTTCGTGGCAGACCATATTCCAAGCTTTGTTTCTCCGAAGGATATTATCAAGGATTACGTCTCCAAAGTACCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

410

Amino Acids

46.98

Weight (kDa)

9.13

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 65 1.1e-08 F-box domain
F-box-like PF12937 26 - 70 6.8e-07 F-box-like
FBA_1 PF07734 120 - 395 1.6e-13 F-box associated beta propeller domain
FBA_3 PF08268 131 - 381 8.5e-26 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000430)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g34920 FvH4_3g37340 FvH4_4g12931 FvH4_4g13090 FvH4_4g13100 FvH4_6g33904 FvH4_6g39874
malus_domestica MD04G1018800.v1.1 MD04G1020000.v1.1 MD04G1020500.v1.1 MD04G1021100.v1.1
prunus_persica Prupe.1G167600_v2.0.a1 Prupe.1G167800_v2.0.a1 Prupe.1G167900_v2.0.a1 Prupe.1G168000_v2.0.a1 Prupe.1G168100_v2.0.a1 Prupe.1G169600_v2.0.a1 Prupe.1G169800_v2.0.a1 Prupe.1G169900_v2.0.a1 Prupe.1G170000_v2.0.a1 Prupe.1G170100_v2.0.a1 Prupe.1G170100_v2.0.a1 Prupe.1G187600_v2.0.a1
pyrus_communis pycom04g01610 pycom04g01630 pycom04g01660
rosa_chinensis RchiOBHm_Chr3g0474061 RchiOBHm_Chr3g0474071 RchiOBHm_Chr3g0494781 RchiOBHm_Chr4g0412631 RchiOBHm_Chr4g0412831 RchiOBHm_Chr4g0412861
rosa_laevigata RLG00000008252 RLG00000008255 RLG00000008272 RLG00000023952 RLG00000026909 RLG00000026910
rosa_multiflora Rmu_co8499129.1_g000001 Rmu_sc0001755.1_g000010 Rmu_sc0001755.1_g000011 Rmu_sc0002068.1_g000006 Rmu_sc0002072.1_g000048 Rmu_sc0002878.1_g000004 Rmu_sc0003022.1_g000007 Rmu_sc0003961.1_g000002 Rmu_sc0010735.1_g000006 Rmu_sc0016141.1_g000002
rosa_roxburghii Rroxscaffold_3G00261940 Rroxscaffold_4G00285070 Rroxscaffold_5G00356800 Rroxscaffold_5G00357100 Rroxscaffold_5G00357120 Rroxscaffold_6G00407500
rosa_rugosa Rorug01G0370900 Rorug03G0138300 Rorug03G0138700 Rorug04G0117100 Rorug04G0117200 Rorug04G0117300 Rorug04G0117300 Rorug07G0011200
rosa_samantha Rh1AG379300 Rh1BG342700 Rh1BG342800 Rh1BG384500 Rh1BG384800 Rh1CG356400 Rh1CG398400 Rh1CG398800 Rh1DG374100 Rh1DG415800 Rh1DG416000 Rh3AG188500 Rh3BG217500 Rh3BG217700 Rh3CG213400 Rh3CG213600 Rh4AG174500 Rh4AG175800 Rh4AG175900 Rh4AG176000 Rh4BG176100 Rh4BG176400 Rh4CG185400 Rh4CG186900 Rh4CG187100 Rh4DG172900 Rh4DG173100 Rh7AG137700 Rh7BG138000 Rh7CG141600
rosa_wichuraiana Rw4G014620 Rw4G014750 Rw4G014760 Rw4G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 168
AccB7I CCANNNNNTGG 1 cut(s) 1159
AccI GTMKAC 1 cut(s) 289
AciI CCGC 2 cut(s) 445, 913
AclWI GGATC 4 cut(s) 359, 411, 424, 986
AcoI YGGCCR 2 cut(s) 312, 910
AcsI RAATTY 1 cut(s) 1040
AcyI GRCGYC 2 cut(s) 169, 942
AfaI GTAC 4 cut(s) 217, 510, 938, 1224
AfiI CCNNNNNNNGG 6 cut(s) 261, 497, 1116, 1117, 1145, 1159
AgsI TTSAA 6 cut(s) 355, 396, 809, 823, 1039, 1124
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 2 cut(s) 102, 1141
AjuI GAANNNNNNNTTGG 2 cut(s) 1168, 1200
AluBI AGCT 3 cut(s) 358, 967, 1179
AluI AGCT 3 cut(s) 358, 967, 1179
Alw26I GTCTC 2 cut(s) 334, 1219
AlwI GGATC 4 cut(s) 359, 411, 424, 986
Ama87I CYCGRG 1 cut(s) 331
AoxI GGCC 3 cut(s) 312, 535, 910
ApoI RAATTY 1 cut(s) 1040
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 4 cut(s) 252, 957, 1011, 1107
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 4 cut(s) 252, 957, 1011, 1107
BalI TGGCCA 1 cut(s) 314
BamHI GGATCC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 338
BauI CACGAG 1 cut(s) 720
BbsI GAAGAC 1 cut(s) 51
BccI CCATC 3 cut(s) 646, 902, 1124
BceAI ACGGC 3 cut(s) 156, 522, 955
BciT130I CCWGG 2 cut(s) 104, 1143
BciVI GTATCC 1 cut(s) 709
BcoDI GTCTC 2 cut(s) 334, 1219
BfaI CTAG 1 cut(s) 1227
BfmI CTRYAG 3 cut(s) 115, 244, 766
BfoI RGCGCY 1 cut(s) 172
BfuI GTATCC 1 cut(s) 709
BglII AGATCT 1 cut(s) 108
BisI GCNGC 1 cut(s) 913
BlsI GCNGC 1 cut(s) 914
Bme1390I CCNGG 2 cut(s) 104, 1143
Bme18I GGWCC 4 cut(s) 252, 957, 1011, 1107
BmeT110I CYCGRG 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 4 cut(s) 252, 957, 1011, 1107
BmiI GGNNCC 6 cut(s) 170, 254, 389, 418, 1012, 1109
BmrFI CCNGG 2 cut(s) 104, 1143
BmrI ACTGGG 1 cut(s) 763
BmsI GCATC 1 cut(s) 1125
BmuI ACTGGG 1 cut(s) 763
BpiI GAAGAC 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 1133
BsaAI YACGTR 1 cut(s) 215
BsaHI GRCGYC 2 cut(s) 169, 942
BsaJI CCNNGG 2 cut(s) 960, 1142
Bsc4I CCNNNNNNNGG 6 cut(s) 261, 497, 1116, 1117, 1145, 1159
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 2 cut(s) 518, 758
Bse3DI GCAATG 1 cut(s) 430
BseBI CCWGG 2 cut(s) 104, 1143
BseDI CCNNGG 2 cut(s) 960, 1142
BseGI GGATG 3 cut(s) 138, 602, 1140
BseLI CCNNNNNNNGG 6 cut(s) 261, 497, 1116, 1117, 1145, 1159
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 15
BseNI ACTGG 2 cut(s) 518, 758
BshFI GGCC 3 cut(s) 314, 537, 912
BshNI GGYRCC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 4 cut(s) 221, 262, 498, 542
BslFI GGGAC 2 cut(s) 552, 840
BslI CCNNNNNNNGG 6 cut(s) 261, 497, 1116, 1117, 1145, 1159
BsmAI GTCTC 2 cut(s) 334, 1219
BsmBI CGTCTC 2 cut(s) 334, 1219
BsmFI GGGAC 2 cut(s) 552, 840
BsnI GGCC 3 cut(s) 314, 537, 912
BsoBI CYCGRG 1 cut(s) 331
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 5 cut(s) 31, 108, 364, 416, 991
BspACI CCGC 2 cut(s) 445, 913
BspANI GGCC 3 cut(s) 314, 537, 912
BspCNI CTCAG 1 cut(s) 16
BspLI GGNNCC 6 cut(s) 170, 254, 389, 418, 1012, 1109
BspPI GGATC 4 cut(s) 359, 411, 424, 986
BspT107I GGYRCC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 430
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 2 cut(s) 518, 758
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 960, 1142
BssMI GATC 5 cut(s) 31, 108, 364, 416, 991
BssNAI GTATAC 1 cut(s) 290
BssNI GRCGYC 2 cut(s) 169, 942
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 960
Bst1107I GTATAC 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 720
Bst2UI CCWGG 2 cut(s) 104, 1143
Bst4CI ACNGT 3 cut(s) 136, 664, 767
Bst6I CTCTTC 1 cut(s) 519
BstACI GRCGYC 2 cut(s) 169, 942
BstBAI YACGTR 1 cut(s) 215
BstC8I GCNNGC 2 cut(s) 226, 815
BstDEI CTNAG 2 cut(s) 24, 883
BstF5I GGATG 3 cut(s) 138, 602, 1140
BstH2I RGCGCY 1 cut(s) 172
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 5 cut(s) 34, 111, 367, 419, 994
BstMAI GTCTC 2 cut(s) 334, 1219
BstMBI GATC 5 cut(s) 31, 108, 364, 416, 991
BstMWI GCNNNNNNNGC 3 cut(s) 333, 435, 444
BstNI CCWGG 2 cut(s) 104, 1143
BstSCI CCNGG 2 cut(s) 102, 1141
BstSFI CTRYAG 3 cut(s) 115, 244, 766
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 3 cut(s) 108, 364, 416
BstYI RGATCY 3 cut(s) 108, 364, 416
BstZ17I GTATAC 1 cut(s) 290
BsuI GTATCC 1 cut(s) 709
BsuRI GGCC 3 cut(s) 314, 537, 912
BtgZI GCGATG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 3 cut(s) 138, 602, 1140
BtsIMutI CAGTG 3 cut(s) 21, 751, 763
Cac8I GCNNGC 2 cut(s) 226, 815
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 4 cut(s) 252, 957, 1011, 1107
CseI GACGC 3 cut(s) 316, 931, 1010
Csp6I GTAC 4 cut(s) 216, 509, 937, 1223
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 4 cut(s) 258, 709, 713, 980
CviQI GTAC 4 cut(s) 216, 509, 937, 1223
DdeI CTNAG 2 cut(s) 24, 883
DinI GGCGCC 1 cut(s) 170
DpnI GATC 5 cut(s) 33, 110, 366, 418, 993
DpnII GATC 5 cut(s) 31, 108, 364, 416, 991
EaeI YGGCCR 2 cut(s) 312, 910
Eam1104I CTCTTC 1 cut(s) 519
EarI CTCTTC 1 cut(s) 519
Eco130I CCWWGG 1 cut(s) 960
Eco24I GRGCYC 1 cut(s) 338
Eco47I GGWCC 4 cut(s) 252, 957, 1011, 1107
Eco88I CYCGRG 1 cut(s) 331
EcoO109I RGGNCCY 3 cut(s) 252, 1011, 1107
EcoRII CCWGG 2 cut(s) 102, 1141
EcoT14I CCWWGG 1 cut(s) 960
EcoT22I ATGCAT 1 cut(s) 714
EcoT38I GRGCYC 1 cut(s) 338
EgeI GGCGCC 1 cut(s) 170
EheI GGCGCC 1 cut(s) 170
ErhI CCWWGG 1 cut(s) 960
Esp3I CGTCTC 2 cut(s) 334, 1219
FaeI CATG 4 cut(s) 261, 712, 716, 983
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 2 cut(s) 552, 840
FatI CATG 4 cut(s) 257, 708, 712, 979
FauNDI CATATG 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 913
FokI GGATG 3 cut(s) 125, 589, 1147
FriOI GRGCYC 1 cut(s) 338
Fsp4HI GCNGC 1 cut(s) 913
FspBI CTAG 1 cut(s) 1227
GlaI GCGC 1 cut(s) 170
GluI GCNGC 1 cut(s) 913
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 172
HaeIII GGCC 3 cut(s) 314, 537, 912
HapII CCGG 4 cut(s) 221, 262, 498, 542
HgaI GACGC 3 cut(s) 316, 931, 1010
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 2 cut(s) 169, 942
Hin1II CATG 4 cut(s) 261, 712, 716, 983
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HindIII AAGCTT 2 cut(s) 356, 1177
HinfI GANTC 4 cut(s) 120, 503, 860, 879
HpaII CCGG 4 cut(s) 221, 262, 498, 542
Hpy166II GTNNAC 3 cut(s) 290, 600, 893
Hpy188I TCNGA 2 cut(s) 364, 1191
Hpy188III TCNNGA 6 cut(s) 35, 331, 352, 857, 946, 1097
Hpy8I GTNNAC 3 cut(s) 290, 600, 893
HpyAV CCTTC 2 cut(s) 719, 1186
HpyCH4III ACNGT 3 cut(s) 136, 664, 767
HpyCH4IV ACGT 6 cut(s) 214, 280, 345, 648, 917, 1212
HpyCH4V TGCA 8 cut(s) 72, 147, 159, 212, 322, 423, 712, 797
HpyF10VI GCNNNNNNNGC 3 cut(s) 333, 435, 444
HpyF3I CTNAG 2 cut(s) 24, 883
HpySE526I ACGT 6 cut(s) 214, 280, 345, 648, 917, 1212
Hsp92I GRCGYC 2 cut(s) 169, 942
Hsp92II CATG 4 cut(s) 261, 712, 716, 983
HspAI GCGC 1 cut(s) 169
KasI GGCGCC 1 cut(s) 168
Kzo9I GATC 5 cut(s) 31, 108, 364, 416, 991
LmnI GCTCC 2 cut(s) 393, 964
LweI GCATC 1 cut(s) 1125
MaeI CTAG 1 cut(s) 1227
MaeII ACGT 6 cut(s) 214, 280, 345, 648, 917, 1212
MaeIII GTNAC 2 cut(s) 400, 779
MalI GATC 5 cut(s) 33, 110, 366, 418, 993
MboI GATC 5 cut(s) 31, 108, 364, 416, 991
MboII GAAGA 6 cut(s) 56, 103, 506, 623, 696, 740
MfeI CAATTG 2 cut(s) 83, 987
MflI RGATCY 3 cut(s) 108, 364, 416
MhlI GDGCHC 1 cut(s) 338
MlsI TGGCCA 1 cut(s) 314
MluNI TGGCCA 1 cut(s) 314
Mly113I GGCGCC 1 cut(s) 169
MlyI GAGTC 2 cut(s) 512, 888
MnlI CCTC 9 cut(s) 258, 443, 522, 879, 881, 942, 1042, 1097, 1110
Mox20I TGGCCA 1 cut(s) 314
Mph1103I ATGCAT 1 cut(s) 714
MscI TGGCCA 1 cut(s) 314
MseI TTAA 2 cut(s) 477, 1231
MslI CAYNNNNRTG 1 cut(s) 396
Msp20I TGGCCA 1 cut(s) 314
MspI CCGG 4 cut(s) 221, 262, 498, 542
MspR9I CCNGG 2 cut(s) 104, 1143
MunI CAATTG 2 cut(s) 83, 987
MvaI CCWGG 2 cut(s) 104, 1143
MwoI GCNNNNNNNGC 3 cut(s) 333, 435, 444
NarI GGCGCC 1 cut(s) 169
NdeI CATATG 1 cut(s) 1006
NdeII GATC 5 cut(s) 31, 108, 364, 416, 991
NlaIII CATG 4 cut(s) 261, 712, 716, 983
NlaIV GGNNCC 6 cut(s) 170, 254, 389, 418, 1012, 1109
NmuCI GTSAC 1 cut(s) 779
NsiI ATGCAT 1 cut(s) 714
PaeR7I CTCGAG 1 cut(s) 331
PfeI GAWTC 2 cut(s) 120, 860
PflMI CCANNNNNTGG 1 cut(s) 1159
PfoI TCCNGGA 1 cut(s) 102
PkrI GCNGC 1 cut(s) 914
PleI GAGTC 2 cut(s) 511, 887
PluTI GGCGCC 1 cut(s) 172
PpsI GAGTC 2 cut(s) 511, 887
Ppu21I YACGTR 1 cut(s) 215
PpuMI RGGWCCY 3 cut(s) 252, 1011, 1107
Psp5II RGGWCCY 3 cut(s) 252, 1011, 1107
Psp6I CCWGG 2 cut(s) 102, 1141
PspGI CCWGG 2 cut(s) 102, 1141
PspN4I GGNNCC 6 cut(s) 170, 254, 389, 418, 1012, 1109
PspPI GGNCC 4 cut(s) 252, 957, 1011, 1107
PspPPI RGGWCCY 3 cut(s) 252, 1011, 1107
PsuI RGATCY 3 cut(s) 108, 364, 416
RsaI GTAC 4 cut(s) 217, 510, 938, 1224
RsaNI GTAC 4 cut(s) 216, 509, 937, 1223
RseI CAYNNNNRTG 1 cut(s) 396
SaqAI TTAA 2 cut(s) 477, 1231
SatI GCNGC 1 cut(s) 913
Sau3AI GATC 5 cut(s) 31, 108, 364, 416, 991
Sau96I GGNCC 4 cut(s) 252, 957, 1011, 1107
SchI GAGTC 2 cut(s) 512, 888
ScrFI CCNGG 2 cut(s) 104, 1143
SduI GDGCHC 1 cut(s) 338
SfaNI GCATC 1 cut(s) 1125
SfcI CTRYAG 3 cut(s) 115, 244, 766
SfoI GGCGCC 1 cut(s) 170
Sfr274I CTCGAG 1 cut(s) 331
SinI GGWCC 4 cut(s) 252, 957, 1011, 1107
SlaI CTCGAG 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 396
SmlI CTYRAG 2 cut(s) 331, 1112
SmoI CTYRAG 2 cut(s) 331, 1112
SsiI CCGC 2 cut(s) 445, 913
SspDI GGCGCC 1 cut(s) 168
SspMI CTAG 1 cut(s) 1227
StyD4I CCNGG 2 cut(s) 102, 1141
StyI CCWWGG 1 cut(s) 960
TaaI ACNGT 3 cut(s) 136, 664, 767
TaiI ACGT 6 cut(s) 217, 283, 348, 651, 920, 1215
TaqI TCGA 1 cut(s) 332
TauI GCSGC 1 cut(s) 915
TfiI GAWTC 2 cut(s) 120, 860
Tru1I TTAA 2 cut(s) 477, 1231
Tru9I TTAA 2 cut(s) 477, 1231
TscAI CASTG 3 cut(s) 28, 758, 770
TseFI GTSAC 1 cut(s) 779
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 7 cut(s) 17, 460, 624, 633, 725, 778, 944
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 3 cut(s) 28, 758, 770
Van91I CCANNNNNTGG 1 cut(s) 1159
VpaK11BI GGWCC 4 cut(s) 252, 957, 1011, 1107
XapI RAATTY 1 cut(s) 1040
XhoI CTCGAG 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 289
XspI CTAG 1 cut(s) 1227
Zsp2I ATGCAT 1 cut(s) 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.