MD10G1235600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000705
Physical Location & Seq
Reverse (-)
33173908 .. 33174811
904 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1235600.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGCCTCATCTGCAATGAAAGGTAGGGCTTGGACCCGAAAAGAAGATGAAGCTCTTTGCAAGGCTTATAGATGGGTCTCAGAAGATAGTGTGAGGGGGATTTCTCAAACAAGTGAAGGTGTTTGGACTCGTGTGTCCAAAAAATACTTAGAGTTCTACGAAGGCACCATTCTACCGAATACCCGAAACCACGAAAGTTGTTCTTCAAGATGGAAGAAACATCTTCATCCAAGTTTGAATAAATGGCATCAAGCACTATTAGCAGCCGCAAGTAGACGTGAAAACAGCGCCAATTACTATGACAAAGTACACCAAGCGGAGGAATTGTATATGAAGGGCAACTCAAAACCCTTTCAGTTTCACGGTTGTTGGGAAATTTGTAAAGGGTGGGTGTTATTTGAAGATCCACATCAACATAGAGTGGATCCTTTGGGAGCTGCATTCCCATATGTAGATATGAATGAAGATGGATCTCCTACCTTCAACAAACAAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

19.13

Weight (kDa)

8.64

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23932 FvH4_3g35672 FvH4_4g18641 FvH4_7g17721
malus_domestica MD03G1177400.v1.1 MD07G1181800.v1.1 MD08G1042000.v1.1 MD08G1109500.v1.1 MD10G1235600.v1.1 MD14G1015600.v1.1 MD17G1218600.v1.1
prunus_persica Prupe.3G094000_v2.0.a1 Prupe.4G233800_v2.0.a1
pyrus_communis pycom05g03000 pycom06g19840 pycom08g03390 pycom08g03420 pycom09g12380 pycom11g05900 pycom13g28390 pycom13g28400 pycom14g03000 pycom16g13550 pycom16g13560 pycom16g25710 pycom17g22320
rosa_chinensis RchiOBHm_Chr2g0111501 RchiOBHm_Chr3g0494851 RchiOBHm_Chr4g0406761 RchiOBHm_Chr6g0260681 RchiOBHm_Chr6g0298551
rosa_laevigata RLG00000002080 RLG00000002250 RLG00000002368 RLG00000012994 RLG00000017948 RLG00000022683
rosa_multiflora Rmu_sc0000441.1_g000135 Rmu_sc0000610.1_g000022 Rmu_sc0000861.1_g000009 Rmu_sc0000938.1_g000012 Rmu_sc0001551.1_g000021 Rmu_sc0001685.1_g000059 Rmu_sc0002132.1_g000058 Rmu_sc0002539.1_g000108 Rmu_sc0002856.1_g000004 Rmu_sc0002868.1_g000022 Rmu_sc0003642.1_g000004 Rmu_sc0005394.1_g000003 Rmu_sc0005742.1_g000002 Rmu_sc0006138.1_g000008 Rmu_sc0006320.1_g000009 Rmu_sc0006616.1_g000006 Rmu_sc0007485.1_g000021 Rmu_sc0008518.1_g000027 Rmu_sc0010999.1_g000017 Rmu_sc0011648.1_g000001 Rmu_sc0013558.1_g000009 Rmu_sc0013665.1_g000001 Rmu_sc0014762.1_g000006 Rmu_ssc0000050.1_g000057
rosa_roxburghii Rroxscaffold_1G00024040
rosa_samantha Rh1CG033000 Rh1CG207600 Rh6CG418600
rosa_wichuraiana Rw1G008980 Rw1G034040 Rw4G013350 Rw4G032310 Rw5G001190 Rw5G026320 Rw5G038830 Rw6G008970 Rw7G034950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 164
AccI GTMKAC 1 cut(s) 274
AciI CCGC 2 cut(s) 267, 317
AclWI GGATC 4 cut(s) 398, 419, 432, 478
AcsI RAATTY 1 cut(s) 375
AfaI GTAC 1 cut(s) 309
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 4 cut(s) 207, 238, 401, 484
AjiI CACGTC 1 cut(s) 278
AluBI AGCT 2 cut(s) 53, 437
AluI AGCT 2 cut(s) 53, 437
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 4 cut(s) 398, 419, 432, 478
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 263, 437
ApoI RAATTY 1 cut(s) 375
AspLEI GCGC 1 cut(s) 290
AspS9I GGNCC 1 cut(s) 33
AvaII GGWCC 1 cut(s) 33
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 164
BauI CACGAG 1 cut(s) 129
BbvI GCAGC 2 cut(s) 275, 424
BccI CCATC 3 cut(s) 66, 204, 461
BcoDI GTCTC 1 cut(s) 82
BfoI RGCGCY 1 cut(s) 291
BisI GCNGC 3 cut(s) 264, 267, 438
BlsI GCNGC 3 cut(s) 265, 268, 439
Bme18I GGWCC 1 cut(s) 33
BmgBI CACGTC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 33
BmiI GGNNCC 3 cut(s) 35, 166, 426
BmsI GCATC 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 21
Bse8I GATNNNNATC 1 cut(s) 408
BseGI GGATG 1 cut(s) 226
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 21
BseMII CTCAG 1 cut(s) 93
BseXI GCAGC 2 cut(s) 275, 424
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 164
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 1 cut(s) 82
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 82
Bsp143I GATC 3 cut(s) 403, 424, 470
BspACI CCGC 2 cut(s) 267, 317
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 92
BspLI GGNNCC 3 cut(s) 35, 166, 426
BspPI GGATC 4 cut(s) 398, 419, 432, 478
BspT107I GGYRCC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 82
BsrDI GCAATG 1 cut(s) 21
BssMI GATC 3 cut(s) 403, 424, 470
BssSI CACGAG 1 cut(s) 129
Bst2BI CACGAG 1 cut(s) 129
Bst4CI ACNGT 1 cut(s) 365
BstDEI CTNAG 2 cut(s) 79, 148
BstF5I GGATG 1 cut(s) 226
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 3 cut(s) 406, 427, 473
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 3 cut(s) 403, 424, 470
BstMWI GCNNNNNNNGC 2 cut(s) 11, 260
BstV1I GCAGC 2 cut(s) 275, 424
BstX2I RGATCY 3 cut(s) 403, 424, 470
BstYI RGATCY 3 cut(s) 403, 424, 470
BsuRI GGCC 1 cut(s) 5
BtrI CACGTC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 226
CfoI GCGC 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 33
Csp6I GTAC 1 cut(s) 308
CspCI CAANNNNNGTGG 2 cut(s) 179, 214
CviJI RGCY 6 cut(s) 5, 29, 53, 65, 266, 437
CviKI_1 RGCY 6 cut(s) 5, 29, 53, 65, 266, 437
CviQI GTAC 1 cut(s) 308
DdeI CTNAG 2 cut(s) 79, 148
DpnI GATC 3 cut(s) 405, 426, 472
DpnII GATC 3 cut(s) 403, 424, 470
DrdI GACNNNNNNGTC 1 cut(s) 133
DseDI GACNNNNNNGTC 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 33
FaiI YATR 8 cut(s) 69, 300, 330, 332, 417, 448, 450, 458
FalI AAGNNNNNCTT 2 cut(s) 187, 219
FauNDI CATATG 1 cut(s) 448
FblI GTMKAC 1 cut(s) 274
Fnu4HI GCNGC 3 cut(s) 264, 267, 438
FokI GGATG 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 264, 267, 438
GlaI GCGC 1 cut(s) 289
GluI GCNGC 3 cut(s) 264, 267, 438
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 290
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HinfI GANTC 1 cut(s) 127
Hpy166II GTNNAC 2 cut(s) 275, 310
Hpy188I TCNGA 1 cut(s) 82
Hpy188III TCNNGA 1 cut(s) 207
Hpy8I GTNNAC 2 cut(s) 275, 310
HpyAV CCTTC 4 cut(s) 110, 155, 328, 490
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 3 cut(s) 14, 60, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 260
HpyF3I CTNAG 2 cut(s) 79, 148
HpySE526I ACGT 1 cut(s) 277
HspAI GCGC 1 cut(s) 288
Kzo9I GATC 3 cut(s) 403, 424, 470
LmnI GCTCC 1 cut(s) 434
Lsp1109I GCAGC 2 cut(s) 275, 424
LweI GCATC 1 cut(s) 256
MaeII ACGT 1 cut(s) 277
MalI GATC 3 cut(s) 405, 426, 472
MboI GATC 3 cut(s) 403, 424, 470
MboII GAAGA 7 cut(s) 56, 95, 195, 215, 226, 413, 476
MflI RGATCY 3 cut(s) 403, 424, 470
MluCI AATT 3 cut(s) 292, 323, 375
MlyI GAGTC 1 cut(s) 121
MnlI CCTC 3 cut(s) 16, 87, 313
Mva1269I GAATGC 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 11, 260
NdeI CATATG 1 cut(s) 448
NdeII GATC 3 cut(s) 403, 424, 470
NlaIV GGNNCC 3 cut(s) 35, 166, 426
PctI GAATGC 1 cut(s) 440
PkrI GCNGC 3 cut(s) 265, 268, 439
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 35, 166, 426
PspPI GGNCC 1 cut(s) 33
PsuI RGATCY 3 cut(s) 403, 424, 470
RsaI GTAC 1 cut(s) 309
RsaNI GTAC 1 cut(s) 308
SatI GCNGC 3 cut(s) 264, 267, 438
Sau3AI GATC 3 cut(s) 403, 424, 470
Sau96I GGNCC 1 cut(s) 33
SchI GAGTC 1 cut(s) 121
SetI ASST 6 cut(s) 25, 55, 121, 280, 439, 482
SfaNI GCATC 1 cut(s) 256
SinI GGWCC 1 cut(s) 33
Sse9I AATT 3 cut(s) 292, 323, 375
SsiI CCGC 2 cut(s) 267, 317
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 1 cut(s) 280
TasI AATT 3 cut(s) 292, 323, 375
TatI WGTACW 1 cut(s) 307
TauI GCSGC 1 cut(s) 269
TseI GCWGC 2 cut(s) 263, 437
TspDTI ATGAA 6 cut(s) 32, 63, 215, 347, 473, 477
VpaK11BI GGWCC 1 cut(s) 33
XapI RAATTY 1 cut(s) 375
XmiI GTMKAC 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.