pycom16g13550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000705
Physical Location & Seq
Forward (+)
9715080 .. 9719546
4467 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g13550.24

Sequence Viewer

Length: 3087 bp
ATGAGGTTATTTAATGTAAATTTTTATCACACTATCATGTTGATTTCTTCATTATTGTATTTATTTTTTTTGAGCTTCTTACTTTTACATTTTAATGATACTGTAACAACGATAATTTTACGTTGTGCTCGTCATTTTACACTATTGACGTATAATATTATACGTGAACATGCTTACATTACAAAATTATCGTTCAATCATTGCCAGTTGCGGAGCCATATAGAGCTTAGGATTGGCCAGGGCTACCCTGCGGTTATCACTTTTAATACCTATTATCTGCAAATATTTGCAAAACCTACCTTGTGCCCCATTTGTCGGTGGTGTTTTGCACTTCCAAAAGGCCACGGTTCGAGACCTTTAGTTATCTTATTTTTTGCCATATTAAAACTAACCCCTATTTTTTGTTATAATTGGCCTTATGTTTTAACTATAAAAACCAGACCAATGTCTTACTTTAAATCAAAACTTGTAAACAAGTTTTGTTTTTACGATATAATGATTTCAAAACTAAGATTTTCTACAAAATTAAGGCTCCACCATTCTTGTTTGATTAAAAATTTTCATGCCAATACAAAACACAAAAACTTTAATTTTCCTCTCATTAGTTTTTCTAGCTCCACCACTGATCATAGCTATGAATACCCTTTTTTTTTTTTTAAAGGTGTTAGCTATGTAAAATATATAGAAAACTTGGATGAACACTCATTATGTACATGTAACCAAGATTTTTTGTATGTTTTAGTATTTTATACTATACATATCATCTATTTTGAAATTTATGTTTCCAATATAATTATATACTTCTTAAAAACTAAAAACAAACCTCCTAGACGGCTATGCTACGAAAATAGCCCGAAAATCGTCTCTAACCGAGGGTTAGGGCAAAGGACTTGTAGGCCCCACTGGACAAAAAATGGCCAAAGGGTACCGTTGGATTTGAATTTTGTTTTTCTTTTTTCCCCAAATTTTTTTTTTTTAATTCTCATTTTTTCCTATAAATACCTAAGCCTTCCTATGTCACATCTCAGTCTTGACTCCGCCGTAGCAAGATATTGTCCGCTTTGGGCCCCGATCACGCCTTCACGGTTTTGTTTCTGGGAACTCACACGAGAACTTCCCAGTGGGTCACCCATCCTACGATTGCTTTCGTCCAAACTCACTGGCTTCAGTAGGCCCAAAGCAGCCAGCCAGCCGGCCCTGGGTACCGTTGGATTTGAATTTTGTTTTTTCTTTTTTAAATACCTAAATATTGTCCGCTTTGGGCCCCGGCCACGCCTTCACGGTTTTGTTTCTAGGAACTCACACGAGAACTTCCCAGTGGGTCACCCATCCTACGATTGCTTTCGTCCTAACTCGCTTAACTTCGGAGTCCTGATGGAATCCGAAGCCAGTGAGTTCGGAAAATGCCTCGTGCTATATGGAGGTAAGCATGGGATGTTACAATCCGCCCCCTTAGGGGCCCGACTTCCTCGTCAACACACTCGCACCACACGGCATAGTGGCTCTGATACCATTCTGACACACCCCGACCTTGGAAGGTCGAAACATGTTGGCCATCACCTCGAAAGTTCCAGCCAAAATGTCTTAGAGGTCTTGGAAAGTTGTGGGAAAGAACTAAAACTCACTGAGTTCGGACCTTCCGAGTTCGACGTTCAGAACTTAGCAAGTTTGAAATTTTTGCAAAAACCAGCGAGATTCGAGTTACAAACGACGGTGTCGGTGGCTGAGGACGACGGAGCTTGTCAGAGAAGGCAGGGAATATTCCGTCAAAGTCGACAGAATATTCTAACGGCGTCAGGTAATGCTATTCCTAACAGCGTCAGTCACACAAACGGCACGTGCCTACGCGTGGGGACGTGTCTAGCCGTGCCTTGGCTAGCGCGTGATGGTGCCGATTTCATGTATTTAAACCCTAGGTTTAAGCAAACTATGGGGTTTTATTTAAGTTTTTGTATATGGAAACGTATCCCGATGACTTACAAAGCCAGGTTAAGCTCAGGGGCTATGACCTTGCGACGTACTTGTGAGTGGGCAATTTATTTTATACCTATACATATTTATAGTTTCCCTAAATGCGTAATTACTCCACTTTTACGTTTATATTGCCAAGTATTCATTATTGTGATATTAAATGTGATAAATGCTACTATATGTTTGGGATATTACTGTCATGACATTTATATGTATATGTGGCCAAGGCCAGTCCTTCACGTGTATGTTCACATCGCACCATTTGCTCACCTTAGATCCAACCGACTTGTATGTGACCATGCTTCTGCACCATCCTGTTCGTGTCAAAGATTTTCTATTCGAACTAGTGTGTCAGCTTTGATGGATGAGCACTTAGCTATACTTGATTATCACATGATTTTTATTACTGTGGCATTGAAATATCATGACTTGGCATATTTCTGGTTATATTGTTGTTATTGTTGTTTACATACCTACGTTACTATATTCGAAAATAAAGGTTTTTGTGTAAACATTGTTTTGCTGAGCCACTCAACTTTGTTTTTCGCCCCTCCAGGACCTAGATACCACTGTTGTGTAATAAGTGTACTTAGCTACTTTGACTACTCTACGGTATCCCATGATTTTTTAAATTTATCCACTTTTTCACATCACTTGCCCTTATGGTTACATCACCTCACGGTGATGGCTAGCATTTCTCACATAATTTTTACCATTCCTACACCATTTCAATTCTCATATTTTTCCTCTTCCAATAATCTTTCCTTCAAATTTTTCAATAATTTTCAAATGGTCTCATTTGCAATGAAAGGTAGGAGATGGGTCTCGGAAGATAGTGTGAGGGGGAGTTCTCAAACAAGTGAAGGTGTTTGGACTCGTGTGTCCAAAAAATACTATGTATGGAAGAAACATCTTCATCTAAGTTTGAATAAATGGCATCAAGTACTGTTAGCAGCCATAAGTAAACATGAAAGCGGCGCCAATTACTACGACGAAGTAAGTGTTTTCACAATTTATTTTAAATATTTAATTATATTACATTTAATTTCACAAATTAATATTCCTTTACGTTTTGTAATTATATTTAATTATATTACATTTAATTTCATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1029

Amino Acids

119.57

Weight (kDa)

9.42

Isoelectric Point (pI)

43.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23932 FvH4_3g35672 FvH4_4g18641 FvH4_7g17721
malus_domestica MD03G1177400.v1.1 MD07G1181800.v1.1 MD08G1042000.v1.1 MD08G1109500.v1.1 MD10G1235600.v1.1 MD14G1015600.v1.1 MD17G1218600.v1.1
prunus_persica Prupe.3G094000_v2.0.a1 Prupe.4G233800_v2.0.a1
pyrus_communis pycom05g03000 pycom06g19840 pycom08g03390 pycom08g03420 pycom09g12380 pycom11g05900 pycom13g28390 pycom13g28400 pycom14g03000 pycom16g13550 pycom16g13560 pycom16g25710 pycom17g22320
rosa_chinensis RchiOBHm_Chr2g0111501 RchiOBHm_Chr3g0494851 RchiOBHm_Chr4g0406761 RchiOBHm_Chr6g0260681 RchiOBHm_Chr6g0298551
rosa_laevigata RLG00000002080 RLG00000002250 RLG00000002368 RLG00000012994 RLG00000017948 RLG00000022683
rosa_multiflora Rmu_sc0000441.1_g000135 Rmu_sc0000610.1_g000022 Rmu_sc0000861.1_g000009 Rmu_sc0000938.1_g000012 Rmu_sc0001551.1_g000021 Rmu_sc0001685.1_g000059 Rmu_sc0002132.1_g000058 Rmu_sc0002539.1_g000108 Rmu_sc0002856.1_g000004 Rmu_sc0002868.1_g000022 Rmu_sc0003642.1_g000004 Rmu_sc0005394.1_g000003 Rmu_sc0005742.1_g000002 Rmu_sc0006138.1_g000008 Rmu_sc0006320.1_g000009 Rmu_sc0006616.1_g000006 Rmu_sc0007485.1_g000021 Rmu_sc0008518.1_g000027 Rmu_sc0010999.1_g000017 Rmu_sc0011648.1_g000001 Rmu_sc0013558.1_g000009 Rmu_sc0013665.1_g000001 Rmu_sc0014762.1_g000006 Rmu_ssc0000050.1_g000057
rosa_roxburghii Rroxscaffold_1G00024040
rosa_samantha Rh1CG033000 Rh1CG207600 Rh6CG418600
rosa_wichuraiana Rw1G008980 Rw1G034040 Rw4G013350 Rw4G032310 Rw5G001190 Rw5G026320 Rw5G038830 Rw6G008970 Rw7G034950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 408
AasI GACNNNNNNGTC 2 cut(s) 1470, 2851
Acc65I GGTACC 2 cut(s) 925, 1202
AccB1I GGYRCC 4 cut(s) 925, 1202, 1889, 2948
AccI GTMKAC 1 cut(s) 1774
AccII CGCG 2 cut(s) 1848, 1882
AciI CCGC 7 cut(s) 211, 251, 1038, 1058, 1255, 1446, 2946
AclWI GGATC 1 cut(s) 2241
AcoI YGGCCR 5 cut(s) 235, 916, 1268, 1552, 2192
AcsI RAATTY 9 cut(s) 19, 556, 774, 940, 964, 1217, 1673, 2602, 2741
AcuI CTGAAG 1 cut(s) 1150
AcvI CACGTG 2 cut(s) 1839, 2212
AcyI GRCGYC 2 cut(s) 1793, 2949
AfaI GTAC 6 cut(s) 712, 927, 1204, 2020, 2559, 2916
AfiI CCNNNNNNNGG 7 cut(s) 315, 1199, 1455, 1456, 1532, 1849, 1872
AflIII ACRYGT 5 cut(s) 713, 1546, 1846, 1856, 2211
AhlI ACTAGT 1 cut(s) 2315
AjiI CACGTC 1 cut(s) 1857
AjnI CCWGG 4 cut(s) 237, 1197, 1985, 2524
AleI CACNNNNGTG 1 cut(s) 2544
Alw21I GWGCWC 2 cut(s) 130, 2343
Alw26I GTCTC 4 cut(s) 346, 868, 2770, 2800
AlwI GGATC 1 cut(s) 2241
ApaI GGGCCC 3 cut(s) 1069, 1266, 1462
ApeKI GCWGC 2 cut(s) 1181, 2924
ApoI RAATTY 9 cut(s) 19, 556, 774, 940, 964, 1217, 1673, 2602, 2741
AseI ATTAAT 1 cut(s) 3027
Asp718I GGTACC 2 cut(s) 925, 1202
AspA2I CCTAGG 1 cut(s) 1913
AspLEI GCGC 2 cut(s) 1882, 2951
AsuC2I CCSGG 1 cut(s) 1267
AsuHPI GGTGA 6 cut(s) 1119, 1316, 1550, 2231, 2636, 2665
AsuII TTCGAA 2 cut(s) 2311, 2460
AsuNHI GCTAGC 2 cut(s) 1876, 2660
AvaII GGWCC 2 cut(s) 1634, 2528
AvrII CCTAGG 1 cut(s) 1913
AxyI CCTNAGG 1 cut(s) 1453
BaeGI GKGCMC 4 cut(s) 308, 1069, 1266, 1462
BaeI ACNNNNGTAYC 2 cut(s) 2527, 2560
BalI TGGCCA 4 cut(s) 237, 918, 1554, 2194
BanI GGYRCC 4 cut(s) 925, 1202, 1889, 2948
BanII GRGCYC 3 cut(s) 1069, 1266, 1462
BauI CACGAG 4 cut(s) 1107, 1304, 1409, 2847
BbrPI CACGTG 2 cut(s) 1839, 2212
Bbv12I GWGCWC 2 cut(s) 130, 2343
BbvCI CCTCAGC 1 cut(s) 1725
BbvI GCAGC 2 cut(s) 1193, 2936
BccI CCATC 9 cut(s) 1139, 1336, 1369, 1563, 1880, 2290, 2326, 2650, 2784
BceAI ACGGC 6 cut(s) 848, 1025, 1508, 1806, 1849, 1850
BciT130I CCWGG 4 cut(s) 239, 1199, 1987, 2526
BciVI GTATCC 2 cut(s) 1976, 2596
BclI TGATCA 1 cut(s) 625
BcnI CCSGG 1 cut(s) 1267
BcoDI GTCTC 4 cut(s) 346, 868, 2770, 2800
BcuI ACTAGT 1 cut(s) 2315
BfaI CTAG 9 cut(s) 612, 828, 1293, 1862, 1877, 1914, 2316, 2532, 2661
BfoI RGCGCY 1 cut(s) 2952
BfuI GTATCC 2 cut(s) 1976, 2596
BisI GCNGC 3 cut(s) 1182, 2925, 2947
BlnI CCTAGG 1 cut(s) 1913
BlpI GCTNAGC 1 cut(s) 2495
BlsI GCNGC 3 cut(s) 1183, 2926, 2948
BmcAI AGTACT 1 cut(s) 2916
Bme1390I CCNGG 5 cut(s) 239, 1199, 1267, 1987, 2526
Bme18I GGWCC 2 cut(s) 1634, 2528
BmgBI CACGTC 1 cut(s) 1857
BmrFI CCNGG 5 cut(s) 239, 1199, 1267, 1987, 2526
BmrI ACTGGG 2 cut(s) 1113, 1310
BmsI GCATC 1 cut(s) 2917
BmtI GCTAGC 2 cut(s) 1880, 2664
BmuI ACTGGG 2 cut(s) 1113, 1310
BoxI GACNNNNGTC 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 2508
Bpu10I CCTNAGC 4 cut(s) 227, 1004, 1725, 1996
Bpu1102I GCTNAGC 1 cut(s) 2495
Bpu14I TTCGAA 2 cut(s) 2311, 2460
BpuMI CCSGG 1 cut(s) 1267
BsaAI YACGTR 3 cut(s) 164, 1839, 2212
BsaHI GRCGYC 2 cut(s) 1793, 2949
BsaI GGTCTC 3 cut(s) 346, 2770, 2800
Bsc4I CCNNNNNNNGG 7 cut(s) 315, 1199, 1455, 1456, 1532, 1849, 1872
Bse118I RCCGGY 1 cut(s) 1192
Bse1I ACTGG 7 cut(s) 205, 908, 1119, 1165, 1316, 1389, 2201
Bse21I CCTNAGG 1 cut(s) 1453
Bse3DI GCAATG 2 cut(s) 199, 2781
BseBI CCWGG 4 cut(s) 239, 1199, 1987, 2526
BseGI GGATG 6 cut(s) 700, 1131, 1328, 1440, 2282, 2341
BseLI CCNNNNNNNGG 7 cut(s) 315, 1199, 1455, 1456, 1532, 1849, 1872
BseMI GCAATG 2 cut(s) 199, 2781
BseMII CTCAG 5 cut(s) 1039, 1617, 1716, 2010, 2486
BseNI ACTGG 7 cut(s) 205, 908, 1119, 1165, 1316, 1389, 2201
BseSI GKGCMC 4 cut(s) 308, 1069, 1266, 1462
BseXI GCAGC 2 cut(s) 1193, 2936
BsgI GTGCAG 1 cut(s) 2262
Bsh1236I CGCG 2 cut(s) 1848, 1882
BshNI GGYRCC 4 cut(s) 925, 1202, 1889, 2948
BsiHKAI GWGCWC 2 cut(s) 130, 2343
BsiSI CCGG 2 cut(s) 1193, 1267
BslFI GGGAC 1 cut(s) 1867
BslI CCNNNNNNNGG 7 cut(s) 315, 1199, 1455, 1456, 1532, 1849, 1872
BsmAI GTCTC 4 cut(s) 346, 868, 2770, 2800
BsmBI CGTCTC 1 cut(s) 868
BsmFI GGGAC 1 cut(s) 1867
Bso31I GGTCTC 3 cut(s) 346, 2770, 2800
Bsp119I TTCGAA 2 cut(s) 2311, 2460
Bsp120I GGGCCC 3 cut(s) 1065, 1262, 1458
Bsp1286I GDGCHC 6 cut(s) 130, 308, 1069, 1266, 1462, 2343
Bsp1407I TGTACA 1 cut(s) 710
Bsp143I GATC 3 cut(s) 625, 1071, 2246
Bsp1720I GCTNAGC 1 cut(s) 2495
BspACI CCGC 7 cut(s) 211, 251, 1038, 1058, 1255, 1446, 2946
BspCNI CTCAG 5 cut(s) 1038, 1618, 1717, 2009, 2487
BspFNI CGCG 2 cut(s) 1848, 1882
BspHI TCATGA 2 cut(s) 2170, 2395
BspOI GCTAGC 2 cut(s) 1880, 2664
BspPI GGATC 1 cut(s) 2241
BspT104I TTCGAA 2 cut(s) 2311, 2460
BspT107I GGYRCC 4 cut(s) 925, 1202, 1889, 2948
BspTNI GGTCTC 3 cut(s) 346, 2770, 2800
BsrDI GCAATG 2 cut(s) 199, 2781
BsrFI RCCGGY 1 cut(s) 1192
BsrGI TGTACA 1 cut(s) 710
BsrI ACTGG 7 cut(s) 205, 908, 1119, 1165, 1316, 1389, 2201
BssAI RCCGGY 1 cut(s) 1192
BssMI GATC 3 cut(s) 625, 1071, 2246
BssNI GRCGYC 2 cut(s) 1793, 2949
BssSI CACGAG 4 cut(s) 1107, 1304, 1409, 2847
BssT1I CCWWGG 4 cut(s) 1531, 1871, 1913, 2195
Bst2BI CACGAG 4 cut(s) 1107, 1304, 1409, 2847
Bst2UI CCWGG 4 cut(s) 239, 1199, 1987, 2526
Bst6I CTCTTC 1 cut(s) 2725
BstACI GRCGYC 2 cut(s) 1793, 2949
BstAPI GCANNNNNTGC 1 cut(s) 2234
BstAUI TGTACA 1 cut(s) 710
BstBAI YACGTR 3 cut(s) 164, 1839, 2212
BstBI TTCGAA 2 cut(s) 2311, 2460
BstC8I GCNNGC 5 cut(s) 1186, 1190, 1194, 1878, 2662
BstDSI CCRYGG 1 cut(s) 343
BstEII GGTNACC 2 cut(s) 1125, 1322
BstF5I GGATG 6 cut(s) 700, 1131, 1328, 1440, 2282, 2341
BstFNI CGCG 2 cut(s) 1848, 1882
BstH2I RGCGCY 1 cut(s) 2952
BstHHI GCGC 2 cut(s) 1882, 2951
BstKTI GATC 3 cut(s) 628, 1074, 2249
BstMAI GTCTC 4 cut(s) 346, 868, 2770, 2800
BstMBI GATC 3 cut(s) 625, 1071, 2246
BstMWI GCNNNNNNNGC 1 cut(s) 2234
BstNI CCWGG 4 cut(s) 239, 1199, 1987, 2526
BstNSI RCATGY 3 cut(s) 173, 717, 1550
BstPAI GACNNNNGTC 1 cut(s) 445
BstPI GGTNACC 2 cut(s) 1125, 1322
BstSCI CCNGG 5 cut(s) 237, 1197, 1265, 1985, 2524
BstSLI GKGCMC 4 cut(s) 308, 1069, 1266, 1462
BstUI CGCG 2 cut(s) 1848, 1882
BstV1I GCAGC 2 cut(s) 1193, 2936
BstX2I RGATCY 1 cut(s) 2246
BstYI RGATCY 1 cut(s) 2246
Bsu36I CCTNAGG 1 cut(s) 1453
BsuI GTATCC 2 cut(s) 1976, 2596
BtgI CCRYGG 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 2209
BtrI CACGTC 1 cut(s) 1857
BtsCI GGATG 6 cut(s) 700, 1131, 1328, 1440, 2282, 2341
BtsIMutI CAGTG 8 cut(s) 621, 901, 1126, 1158, 1323, 1396, 1623, 2539
Cac8I GCNNGC 5 cut(s) 1186, 1190, 1194, 1878, 2662
CciI TCATGA 2 cut(s) 2170, 2395
CfoI GCGC 2 cut(s) 1882, 2951
Cfr10I RCCGGY 1 cut(s) 1192
CseI GACGC 2 cut(s) 1782, 1807
Csp6I GTAC 6 cut(s) 711, 926, 1203, 2019, 2558, 2915
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
CviQI GTAC 6 cut(s) 711, 926, 1203, 2019, 2558, 2915
DinI GGCGCC 1 cut(s) 2950
DpnI GATC 3 cut(s) 627, 1073, 2248
DpnII GATC 3 cut(s) 625, 1071, 2246
DraI TTTAAA 6 cut(s) 457, 658, 1237, 1908, 2601, 2992
DrdI GACNNNNNNGTC 2 cut(s) 1470, 2851
DseDI GACNNNNNNGTC 2 cut(s) 1470, 2851
EaeI YGGCCR 5 cut(s) 235, 916, 1268, 1552, 2192
Eam1104I CTCTTC 1 cut(s) 2725
EarI CTCTTC 1 cut(s) 2725
EciI GGCGGA 2 cut(s) 1027, 1435
Eco130I CCWWGG 4 cut(s) 1531, 1871, 1913, 2195
Eco24I GRGCYC 3 cut(s) 1069, 1266, 1462
Eco31I GGTCTC 3 cut(s) 346, 2770, 2800
Eco47I GGWCC 2 cut(s) 1634, 2528
Eco57I CTGAAG 1 cut(s) 1150
Eco72I CACGTG 2 cut(s) 1839, 2212
Eco81I CCTNAGG 1 cut(s) 1453
Eco91I GGTNACC 2 cut(s) 1125, 1322
EcoO109I RGGNCCY 5 cut(s) 897, 1066, 1263, 1458, 2528
EcoO65I GGTNACC 2 cut(s) 1125, 1322
EcoRII CCWGG 4 cut(s) 237, 1197, 1985, 2524
EcoT14I CCWWGG 4 cut(s) 1531, 1871, 1913, 2195
EcoT38I GRGCYC 3 cut(s) 1069, 1266, 1462
EgeI GGCGCC 1 cut(s) 2950
EheI GGCGCC 1 cut(s) 2950
ErhI CCWWGG 4 cut(s) 1531, 1871, 1913, 2195
Esp3I CGTCTC 1 cut(s) 868
FaqI GGGAC 1 cut(s) 1867
FbaI TGATCA 1 cut(s) 625
FblI GTMKAC 1 cut(s) 1774
Fnu4HI GCNGC 3 cut(s) 1182, 2925, 2947
FokI GGATG 6 cut(s) 707, 1118, 1315, 1447, 2269, 2348
FriOI GRGCYC 3 cut(s) 1069, 1266, 1462
Fsp4HI GCNGC 3 cut(s) 1182, 2925, 2947
FspBI CTAG 9 cut(s) 612, 828, 1293, 1862, 1877, 1914, 2316, 2532, 2661
GlaI GCGC 2 cut(s) 1881, 2950
GluI GCNGC 3 cut(s) 1182, 2925, 2947
GsuI CTGGAG 1 cut(s) 2508
HaeII RGCGCY 1 cut(s) 2952
HapII CCGG 2 cut(s) 1193, 1267
HgaI GACGC 2 cut(s) 1782, 1807
HhaI GCGC 2 cut(s) 1882, 2951
Hin1I GRCGYC 2 cut(s) 1793, 2949
Hin6I GCGC 2 cut(s) 1880, 2949
HinP1I GCGC 2 cut(s) 1880, 2949
HincII GTYRAC 2 cut(s) 1475, 1775
HindII GTYRAC 2 cut(s) 1475, 1775
HinfI GANTC 5 cut(s) 1034, 1368, 1379, 1695, 2845
HpaII CCGG 2 cut(s) 1193, 1267
HphI GGTGA 6 cut(s) 1119, 1316, 1550, 2231, 2636, 2665
Hpy166II GTNNAC 9 cut(s) 167, 472, 1475, 1775, 2221, 2438, 2482, 2558, 2936
Hpy188III TCNNGA 6 cut(s) 351, 1031, 1372, 1969, 2171, 2396
Hpy8I GTNNAC 9 cut(s) 167, 472, 1475, 1775, 2221, 2438, 2482, 2558, 2936
Hpy99I CGWCG 5 cut(s) 1652, 1714, 1736, 2019, 2966
HpyAV CCTTC 9 cut(s) 1019, 1089, 1286, 1530, 1647, 1743, 2216, 2746, 2828
HpyCH4V TGCA 6 cut(s) 280, 290, 329, 1681, 2279, 2774
HpyF10VI GCNNNNNNNGC 1 cut(s) 2234
Hsp92I GRCGYC 2 cut(s) 1793, 2949
HspAI GCGC 2 cut(s) 1880, 2949
KasI GGCGCC 1 cut(s) 2948
KpnI GGTACC 2 cut(s) 929, 1206
KroI GCCGGC 1 cut(s) 1192
KroNI GCCGGC 1 cut(s) 1194
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 3 cut(s) 625, 1071, 2246
LmnI GCTCC 4 cut(s) 213, 537, 620, 1736
Lsp1109I GCAGC 2 cut(s) 1193, 2936
LweI GCATC 1 cut(s) 2917
MaeI CTAG 9 cut(s) 612, 828, 1293, 1862, 1877, 1914, 2316, 2532, 2661
MalI GATC 3 cut(s) 627, 1073, 2248
MboI GATC 3 cut(s) 625, 1071, 2246
MboII GAAGA 5 cut(s) 39, 2712, 2813, 2876, 2887
MflI RGATCY 1 cut(s) 2246
MhlI GDGCHC 6 cut(s) 130, 308, 1069, 1266, 1462, 2343
MlsI TGGCCA 4 cut(s) 237, 918, 1554, 2194
MluI ACGCGT 1 cut(s) 1846
MluNI TGGCCA 4 cut(s) 237, 918, 1554, 2194
Mly113I GGCGCC 1 cut(s) 2949
MlyI GAGTC 3 cut(s) 1028, 1377, 2839
MmeI TCCRAC 3 cut(s) 912, 1189, 2274
Mox20I TGGCCA 4 cut(s) 237, 918, 1554, 2194
MroNI GCCGGC 1 cut(s) 1192
MscI TGGCCA 4 cut(s) 237, 918, 1554, 2194
MslI CAYNNNNRTG 8 cut(s) 35, 93, 633, 2120, 2180, 2214, 2544, 2654
Msp20I TGGCCA 4 cut(s) 237, 918, 1554, 2194
MspI CCGG 2 cut(s) 1193, 1267
MspR9I CCNGG 5 cut(s) 239, 1199, 1267, 1987, 2526
MvaI CCWGG 4 cut(s) 239, 1199, 1987, 2526
MvnI CGCG 2 cut(s) 1848, 1882
MwoI GCNNNNNNNGC 1 cut(s) 2234
NaeI GCCGGC 1 cut(s) 1194
NarI GGCGCC 1 cut(s) 2949
NciI CCSGG 1 cut(s) 1267
NdeII GATC 3 cut(s) 625, 1071, 2246
NgoMIV GCCGGC 1 cut(s) 1192
NheI GCTAGC 2 cut(s) 1876, 2660
NmuCI GTSAC 5 cut(s) 1017, 1125, 1322, 1823, 2264
NspI RCATGY 3 cut(s) 173, 717, 1550
NspV TTCGAA 2 cut(s) 2311, 2460
OliI CACNNNNGTG 1 cut(s) 2544
PagI TCATGA 2 cut(s) 2170, 2395
PasI CCCWGGG 1 cut(s) 1198
PciI ACATGT 2 cut(s) 713, 1546
PcsI WCGNNNNNNNCGW 2 cut(s) 127, 1638
PdiI GCCGGC 1 cut(s) 1194
PfeI GAWTC 2 cut(s) 1379, 1695
PflFI GACNNNGTC 1 cut(s) 1714
PfoI TCCNGGA 1 cut(s) 2524
PkrI GCNGC 3 cut(s) 1183, 2926, 2948
PleI GAGTC 3 cut(s) 1028, 1376, 2839
PluTI GGCGCC 1 cut(s) 2952
PmaCI CACGTG 2 cut(s) 1839, 2212
PmlI CACGTG 2 cut(s) 1839, 2212
PpsI GAGTC 3 cut(s) 1028, 1376, 2839
Ppu21I YACGTR 3 cut(s) 164, 1839, 2212
PpuMI RGGWCCY 1 cut(s) 2528
PscI ACATGT 2 cut(s) 713, 1546
PshAI GACNNNNGTC 1 cut(s) 445
PshBI ATTAAT 1 cut(s) 3027
PsiI TTATAA 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 2528
Psp6I CCWGG 4 cut(s) 237, 1197, 1985, 2524
PspCI CACGTG 2 cut(s) 1839, 2212
PspEI GGTNACC 2 cut(s) 1125, 1322
PspGI CCWGG 4 cut(s) 237, 1197, 1985, 2524
PspOMI GGGCCC 3 cut(s) 1065, 1262, 1458
PspPPI RGGWCCY 1 cut(s) 2528
PsuI RGATCY 1 cut(s) 2246
PsyI GACNNNGTC 1 cut(s) 1714
RsaI GTAC 6 cut(s) 712, 927, 1204, 2020, 2559, 2916
RsaNI GTAC 6 cut(s) 711, 926, 1203, 2019, 2558, 2915
RseI CAYNNNNRTG 8 cut(s) 35, 93, 633, 2120, 2180, 2214, 2544, 2654
SalI GTCGAC 1 cut(s) 1773
SatI GCNGC 3 cut(s) 1182, 2925, 2947
Sau3AI GATC 3 cut(s) 625, 1071, 2246
ScaI AGTACT 1 cut(s) 2916
SchI GAGTC 3 cut(s) 1028, 1377, 2839
ScrFI CCNGG 5 cut(s) 239, 1199, 1267, 1987, 2526
SduI GDGCHC 6 cut(s) 130, 308, 1069, 1266, 1462, 2343
SfaNI GCATC 1 cut(s) 2917
SfoI GGCGCC 1 cut(s) 2950
SfuI TTCGAA 2 cut(s) 2311, 2460
SinI GGWCC 2 cut(s) 1634, 2528
SmiMI CAYNNNNRTG 8 cut(s) 35, 93, 633, 2120, 2180, 2214, 2544, 2654
SpeI ACTAGT 1 cut(s) 2315
SsiI CCGC 7 cut(s) 211, 251, 1038, 1058, 1255, 1446, 2946
SspDI GGCGCC 1 cut(s) 2948
SspI AATATT 7 cut(s) 157, 285, 1249, 1761, 1783, 2996, 3031
SspMI CTAG 9 cut(s) 612, 828, 1293, 1862, 1877, 1914, 2316, 2532, 2661
StyD4I CCNGG 5 cut(s) 237, 1197, 1265, 1985, 2524
StyI CCWWGG 4 cut(s) 1531, 1871, 1913, 2195
TaqI TCGA 8 cut(s) 350, 1541, 1563, 1647, 1698, 1774, 2311, 2460
TatI WGTACW 3 cut(s) 710, 2557, 2914
TauI GCSGC 1 cut(s) 2949
TfiI GAWTC 2 cut(s) 1379, 1695
TscAI CASTG 8 cut(s) 628, 908, 1126, 1165, 1323, 1396, 1630, 2546
TseFI GTSAC 5 cut(s) 1017, 1125, 1322, 1823, 2264
TseI GCWGC 2 cut(s) 1181, 2924
Tsp45I GTSAC 5 cut(s) 1017, 1125, 1322, 1823, 2264
TspGWI ACGGA 2 cut(s) 1749, 1754
TspRI CASTG 8 cut(s) 628, 908, 1126, 1165, 1323, 1396, 1630, 2546
Tth111I GACNNNGTC 1 cut(s) 1714
VpaK11BI GGWCC 2 cut(s) 1634, 2528
VspI ATTAAT 1 cut(s) 3027
XapI RAATTY 9 cut(s) 19, 556, 774, 940, 964, 1217, 1673, 2602, 2741
XceI RCATGY 3 cut(s) 173, 717, 1550
XmaJI CCTAGG 1 cut(s) 1913
XmiI GTMKAC 1 cut(s) 1774
XspI CTAG 9 cut(s) 612, 828, 1293, 1862, 1877, 1914, 2316, 2532, 2661
ZrmI AGTACT 1 cut(s) 2916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.