Rmu_ssc0000050.1_g000057

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000050.1
OG0000705
Physical Location & Seq
Reverse (-)
267799 .. 268770
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000050.1_g000057.1.cds

Sequence Viewer

Length: 681 bp
atgccaaaagaagatgaagctcttactattgcttatatcaaagtctcggaagatatggagaagggtaccaaccaatcaaaggaagggctttgggcacgaatccaacaaacatacttggagtattatgaaggcaccaaacttccgaaggacgactctggaggacaaccatttaagtttggtaattgttataatttgtgcaaaaattggctgacttttcaagatccacctcaagaacaatttggtccaacactggttcaacgaaacacctcaatttctttggaggatgatgaggatcccattgaggattctcctacttcatctcaaatgagaattcctagacctatgggaagaaatgcagcaagaaaaacgaggcaaaagagcaaagaggcagaagtcaaagccattggagaagaaatggttgcacaaatgaaagaggcggaagaggaaatgaagagaagagagaagaaaaggaaggatcgaagagaggttgctaggttagaggctattttaatgatgcaaaccatcaattttactccaaggataaagagtattttgatgggaagaaaagagaagctctccaccaactcccaggacgtgaattgtttccaaattccggtgagatatccaatgtctattgtccacaaatgtcatccgatgaagatgaagcttaatttttattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

26.36

Weight (kDa)

8.9

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23932 FvH4_3g35672 FvH4_4g18641 FvH4_7g17721
malus_domestica MD03G1177400.v1.1 MD07G1181800.v1.1 MD08G1042000.v1.1 MD08G1109500.v1.1 MD10G1235600.v1.1 MD14G1015600.v1.1 MD17G1218600.v1.1
prunus_persica Prupe.3G094000_v2.0.a1 Prupe.4G233800_v2.0.a1
pyrus_communis pycom05g03000 pycom06g19840 pycom08g03390 pycom08g03420 pycom09g12380 pycom11g05900 pycom13g28390 pycom13g28400 pycom14g03000 pycom16g13550 pycom16g13560 pycom16g25710 pycom17g22320
rosa_chinensis RchiOBHm_Chr2g0111501 RchiOBHm_Chr3g0494851 RchiOBHm_Chr4g0406761 RchiOBHm_Chr6g0260681 RchiOBHm_Chr6g0298551
rosa_laevigata RLG00000002080 RLG00000002250 RLG00000002368 RLG00000012994 RLG00000017948 RLG00000022683
rosa_multiflora Rmu_sc0000441.1_g000135 Rmu_sc0000610.1_g000022 Rmu_sc0000861.1_g000009 Rmu_sc0000938.1_g000012 Rmu_sc0001551.1_g000021 Rmu_sc0001685.1_g000059 Rmu_sc0002132.1_g000058 Rmu_sc0002539.1_g000108 Rmu_sc0002856.1_g000004 Rmu_sc0002868.1_g000022 Rmu_sc0003642.1_g000004 Rmu_sc0005394.1_g000003 Rmu_sc0005742.1_g000002 Rmu_sc0006138.1_g000008 Rmu_sc0006320.1_g000009 Rmu_sc0006616.1_g000006 Rmu_sc0007485.1_g000021 Rmu_sc0008518.1_g000027 Rmu_sc0010999.1_g000017 Rmu_sc0011648.1_g000001 Rmu_sc0013558.1_g000009 Rmu_sc0013665.1_g000001 Rmu_sc0014762.1_g000006 Rmu_ssc0000050.1_g000057
rosa_roxburghii Rroxscaffold_1G00024040
rosa_samantha Rh1CG033000 Rh1CG207600 Rh6CG418600
rosa_wichuraiana Rw1G008980 Rw1G034040 Rw4G013350 Rw4G032310 Rw5G001190 Rw5G026320 Rw5G038830 Rw6G008970 Rw7G034950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 189
Acc65I GGTACC 1 cut(s) 65
AccB1I GGYRCC 2 cut(s) 65, 131
AciI CCGC 1 cut(s) 437
AclWI GGATC 4 cut(s) 215, 287, 300, 483
AcsI RAATTY 2 cut(s) 330, 609
AfaI GTAC 1 cut(s) 67
AfiI CCNNNNNNNGG 2 cut(s) 79, 613
AgsI TTSAA 2 cut(s) 218, 257
AjiI CACGTC 1 cut(s) 595
AjnI CCWGG 1 cut(s) 588
AluBI AGCT 3 cut(s) 20, 574, 667
AluI AGCT 3 cut(s) 20, 574, 667
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 4 cut(s) 215, 287, 300, 483
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 330, 609
Asp700I GAANNNNTTC 1 cut(s) 602
Asp718I GGTACC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 1 cut(s) 628
AvaII GGWCC 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 97
BamHI GGATCC 1 cut(s) 292
BanI GGYRCC 2 cut(s) 65, 131
BbvI GCAGC 1 cut(s) 368
BccI CCATC 2 cut(s) 530, 550
BciT130I CCWGG 1 cut(s) 590
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 2 cut(s) 336, 492
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 590
Bme18I GGWCC 1 cut(s) 242
BmgBI CACGTC 1 cut(s) 595
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 3 cut(s) 67, 133, 294
BmrFI CCNGG 1 cut(s) 590
BmsI GCATC 1 cut(s) 504
BplI GAGNNNNNCTC 2 cut(s) 560, 592
BpmI CTGGAG 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 213
BsaBI GATNNNNATC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 536, 588
BsaWI WCCGGW 1 cut(s) 613
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 613
Bse1I ACTGG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 291
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 2 cut(s) 536, 588
BseGI GGATG 2 cut(s) 289, 649
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 2 cut(s) 79, 613
BseNI ACTGG 1 cut(s) 255
BseSI GKGCMC 1 cut(s) 97
BseXI GCAGC 1 cut(s) 368
BshNI GGYRCC 2 cut(s) 65, 131
BsiSI CCGG 1 cut(s) 614
BslI CCNNNNNNNGG 2 cut(s) 79, 613
BsmAI GTCTC 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 97
Bsp143I GATC 3 cut(s) 220, 292, 475
BspACI CCGC 1 cut(s) 437
BspLI GGNNCC 3 cut(s) 67, 133, 294
BspPI GGATC 4 cut(s) 215, 287, 300, 483
BspT107I GGYRCC 2 cut(s) 65, 131
BsrI ACTGG 1 cut(s) 255
BssECI CCNNGG 2 cut(s) 536, 588
BssMI GATC 3 cut(s) 220, 292, 475
BssT1I CCWWGG 1 cut(s) 536
Bst2UI CCWGG 1 cut(s) 590
Bst6I CTCTTC 4 cut(s) 435, 446, 451, 475
BstF5I GGATG 2 cut(s) 289, 649
BstKTI GATC 3 cut(s) 223, 295, 478
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 3 cut(s) 220, 292, 475
BstNI CCWGG 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 588
BstSLI GKGCMC 1 cut(s) 97
BstV1I GCAGC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 220, 292
BstYI RGATCY 2 cut(s) 220, 292
BtrI CACGTC 1 cut(s) 595
BtsCI GGATG 2 cut(s) 289, 649
BtsIMutI CAGTG 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 242
Csp6I GTAC 1 cut(s) 66
CviJI RGCY 7 cut(s) 20, 88, 208, 401, 503, 574, 667
CviKI_1 RGCY 7 cut(s) 20, 88, 208, 401, 503, 574, 667
CviQI GTAC 1 cut(s) 66
DpnI GATC 3 cut(s) 222, 294, 477
DpnII GATC 3 cut(s) 220, 292, 475
Eam1104I CTCTTC 4 cut(s) 435, 446, 451, 475
EarI CTCTTC 4 cut(s) 435, 446, 451, 475
EciI GGCGGA 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 536
Eco32I GATATC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 588
EcoRV GATATC 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 536
FaiI YATR 6 cut(s) 36, 56, 112, 126, 189, 344
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 296, 636
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 2 cut(s) 336, 492
GluI GCNGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 177
HapII CCGG 1 cut(s) 614
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 3 cut(s) 99, 152, 305
HpaII CCGG 1 cut(s) 614
HphI GGTGA 1 cut(s) 628
Hpy166II GTNNAC 1 cut(s) 640
Hpy188I TCNGA 3 cut(s) 49, 144, 654
Hpy188III TCNNGA 3 cut(s) 156, 218, 230
Hpy8I GTNNAC 1 cut(s) 640
HpyAV CCTTC 5 cut(s) 55, 77, 122, 139, 466
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 4 cut(s) 198, 356, 422, 517
HpySE526I ACGT 1 cut(s) 594
KpnI GGTACC 1 cut(s) 69
Kzo9I GATC 3 cut(s) 220, 292, 475
LpnPI CCDG 5 cut(s) 141, 236, 575, 602, 627
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 1 cut(s) 504
MaeI CTAG 2 cut(s) 336, 492
MaeII ACGT 1 cut(s) 594
MalI GATC 3 cut(s) 222, 294, 477
MboI GATC 3 cut(s) 220, 292, 475
MflI RGATCY 2 cut(s) 220, 292
MhlI GDGCHC 1 cut(s) 97
MlyI GAGTC 1 cut(s) 146
MmeI TCCRAC 2 cut(s) 127, 269
MroXI GAANNNNTTC 1 cut(s) 602
MseI TTAA 4 cut(s) 171, 509, 669, 679
MspI CCGG 1 cut(s) 614
MspR9I CCNGG 1 cut(s) 590
MvaI CCWGG 1 cut(s) 590
NdeII GATC 3 cut(s) 220, 292, 475
NlaIV GGNNCC 3 cut(s) 67, 133, 294
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 2 cut(s) 99, 305
PkrI GCNGC 1 cut(s) 358
PleI GAGTC 1 cut(s) 146
PpsI GAGTC 1 cut(s) 146
PsiI TTATAA 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 3 cut(s) 67, 133, 294
PspPI GGNCC 1 cut(s) 242
PsuI RGATCY 2 cut(s) 220, 292
RsaI GTAC 1 cut(s) 67
RsaNI GTAC 1 cut(s) 66
SaqAI TTAA 4 cut(s) 171, 509, 669, 679
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 3 cut(s) 220, 292, 475
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 590
SduI GDGCHC 1 cut(s) 97
SetI ASST 9 cut(s) 22, 229, 269, 343, 489, 497, 576, 597, 669
SfaNI GCATC 1 cut(s) 504
SinI GGWCC 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 228
SmoI CTYRAG 1 cut(s) 228
SsiI CCGC 1 cut(s) 437
SspMI CTAG 2 cut(s) 336, 492
StyD4I CCNGG 1 cut(s) 588
StyI CCWWGG 1 cut(s) 536
TaiI ACGT 1 cut(s) 597
TaqI TCGA 1 cut(s) 478
TfiI GAWTC 2 cut(s) 99, 305
Tru1I TTAA 4 cut(s) 171, 509, 669, 679
Tru9I TTAA 4 cut(s) 171, 509, 669, 679
TscAI CASTG 1 cut(s) 255
TseI GCWGC 1 cut(s) 356
TspDTI ATGAA 7 cut(s) 30, 141, 306, 443, 464, 671, 677
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 2 cut(s) 330, 609
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 2 cut(s) 336, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.