RchiOBHm_Chr6g0260681

regulation of endocannabinoid signaling pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000705
Physical Location & Seq
Reverse (-)
15641334 .. 15642436
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23378

Sequence Viewer

Length: 930 bp
ATGGAGAATTCTGAAAAGACAATAAGAGGACTTGCATTCTCCGGTCCAGAAGACGACGCACTATGCAAGGCATTTTTGTATGTAAGTCAAGATTCTGCTATTGGGTCCGGGCAAACAAAGGTTGTATTTTGGCGACGAATTCATGCAAAATGGGAAGAATTGTATGTTGCAGAAACAGGGCTTGCCCCAAAGAGAGTACCAGGTAGTCTCCGTGCTCGATTCAAAGCTATAAAAACTCAGTGTACTAAATATGCTGCAAGAGTAACAGAGACAAAACGAAGACGGATTAGCGGTTTTACTGAAGCCGACATTATGGAACAAGCAAATATAAGCTTTAAAAATAAGGAGAAAACTGTGTTTGCTCATCATCATTGTTGGAGATATTTGAAAGATGCTATTACATGGAAAATTCCTGCGGGAACCGACAACAACTCACTCTACGGCAATTCATCGTCGCAGTTTACCGAAACAGAAGAAACACACACTTCACTTGATGGAGATGGAGATGAAGAGCCGCCGGTTAGTATTCCGGCCACTAACCCCAATCAAAGGCCCATCGGGAAAAAGGCTGCAAAGAGGAAGATTATTGAAGAGGGGCAAGCTTCTGATATGGCTCAAGAACTTCGGCGATATAATAACATCATGGAAGCTGAAGCTATTAGGAAGAAAGAGAAGGACAAACATATGATACGAGTATTTCAAGAGTCCGCAAGACGGGAAGAAGAAAAGGCAGATTTGGAGTATTTGAAAATAGATACCACCTATATGCCTCCAGAGCAGAGAGAATTCTTCGAGCATAAGAAAACTGAGATTTATAATAAAATGAGATTCCGTGGCTCCGATTCCTCAAGCTCCGGTAACCCATCAGTAACCTCAGACTTCGATACGTCATCAGAATATCATGTTGATCCGAATCCTGGTCCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

35.35

Weight (kDa)

7.7

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 120 - 271 4.9e-15 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g23932 FvH4_3g35672 FvH4_4g18641 FvH4_7g17721
malus_domestica MD03G1177400.v1.1 MD07G1181800.v1.1 MD08G1042000.v1.1 MD08G1109500.v1.1 MD10G1235600.v1.1 MD14G1015600.v1.1 MD17G1218600.v1.1
prunus_persica Prupe.3G094000_v2.0.a1 Prupe.4G233800_v2.0.a1
pyrus_communis pycom05g03000 pycom06g19840 pycom08g03390 pycom08g03420 pycom09g12380 pycom11g05900 pycom13g28390 pycom13g28400 pycom14g03000 pycom16g13550 pycom16g13560 pycom16g25710 pycom17g22320
rosa_chinensis RchiOBHm_Chr2g0111501 RchiOBHm_Chr3g0494851 RchiOBHm_Chr4g0406761 RchiOBHm_Chr6g0260681 RchiOBHm_Chr6g0298551
rosa_laevigata RLG00000002080 RLG00000002250 RLG00000002368 RLG00000012994 RLG00000017948 RLG00000022683
rosa_multiflora Rmu_sc0000441.1_g000135 Rmu_sc0000610.1_g000022 Rmu_sc0000861.1_g000009 Rmu_sc0000938.1_g000012 Rmu_sc0001551.1_g000021 Rmu_sc0001685.1_g000059 Rmu_sc0002132.1_g000058 Rmu_sc0002539.1_g000108 Rmu_sc0002856.1_g000004 Rmu_sc0002868.1_g000022 Rmu_sc0003642.1_g000004 Rmu_sc0005394.1_g000003 Rmu_sc0005742.1_g000002 Rmu_sc0006138.1_g000008 Rmu_sc0006320.1_g000009 Rmu_sc0006616.1_g000006 Rmu_sc0007485.1_g000021 Rmu_sc0008518.1_g000027 Rmu_sc0010999.1_g000017 Rmu_sc0011648.1_g000001 Rmu_sc0013558.1_g000009 Rmu_sc0013665.1_g000001 Rmu_sc0014762.1_g000006 Rmu_ssc0000050.1_g000057
rosa_roxburghii Rroxscaffold_1G00024040
rosa_samantha Rh1CG033000 Rh1CG207600 Rh6CG418600
rosa_wichuraiana Rw1G008980 Rw1G034040 Rw4G013350 Rw4G032310 Rw5G001190 Rw5G026320 Rw5G038830 Rw6G008970 Rw7G034950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
AciI CCGC 4 cut(s) 291, 416, 515, 708
AclWI GGATC 1 cut(s) 902
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 4 cut(s) 7, 138, 408, 785
AcuI CTGAAG 2 cut(s) 321, 672
AfaI GTAC 2 cut(s) 198, 244
AfiI CCNNNNNNNGG 3 cut(s) 549, 714, 917
AgsI TTSAA 5 cut(s) 223, 388, 590, 701, 748
AjnI CCWGG 2 cut(s) 199, 916
AluBI AGCT 6 cut(s) 227, 333, 602, 650, 656, 852
AluI AGCT 6 cut(s) 227, 333, 602, 650, 656, 852
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 2 cut(s) 212, 263
AlwI GGATC 1 cut(s) 902
AoxI GGCC 2 cut(s) 531, 551
ApeKI GCWGC 2 cut(s) 254, 569
ApoI RAATTY 4 cut(s) 7, 138, 408, 785
AspS9I GGNCC 4 cut(s) 44, 105, 552, 920
AsuC2I CCSGG 1 cut(s) 109
AvaII GGWCC 3 cut(s) 44, 105, 920
BbsI GAAGAC 2 cut(s) 57, 286
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 241, 556
BccI CCATC 4 cut(s) 488, 494, 563, 871
BceAI ACGGC 1 cut(s) 457
BciT130I CCWGG 2 cut(s) 201, 918
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 212, 263
BisI GCNGC 3 cut(s) 255, 515, 570
BlsI GCNGC 3 cut(s) 256, 516, 571
Bme1390I CCNGG 3 cut(s) 109, 201, 918
Bme18I GGWCC 3 cut(s) 44, 105, 920
BmgT120I GGNCC 4 cut(s) 44, 105, 552, 920
BmiI GGNNCC 3 cut(s) 106, 421, 838
BmrFI CCNGG 3 cut(s) 109, 201, 918
BmsI GCATC 1 cut(s) 382
BpiI GAAGAC 2 cut(s) 57, 286
BpmI CTGGAG 1 cut(s) 756
BpuEI CTTGAG 2 cut(s) 600, 832
BpuMI CCSGG 1 cut(s) 109
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 1 cut(s) 832
BsaWI WCCGGW 2 cut(s) 41, 854
Bsc4I CCNNNNNNNGG 3 cut(s) 549, 714, 917
Bse118I RCCGGY 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 2 cut(s) 201, 918
BseDI CCNNGG 1 cut(s) 832
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 3 cut(s) 549, 714, 917
BseMII CTCAG 3 cut(s) 251, 798, 888
BseXI GCAGC 2 cut(s) 241, 556
BshFI GGCC 2 cut(s) 533, 553
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 5 cut(s) 42, 108, 518, 530, 855
BslI CCNNNNNNNGG 3 cut(s) 549, 714, 917
BsmAI GTCTC 2 cut(s) 212, 263
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 2 cut(s) 533, 553
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 1 cut(s) 907
BspACI CCGC 4 cut(s) 291, 416, 515, 708
BspANI GGCC 2 cut(s) 533, 553
BspCNI CTCAG 3 cut(s) 250, 799, 887
BspLI GGNNCC 3 cut(s) 106, 421, 838
BspPI GGATC 1 cut(s) 902
BspQI GCTCTTC 1 cut(s) 504
BsrFI RCCGGY 1 cut(s) 517
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 832
BssMI GATC 1 cut(s) 907
Bst2UI CCWGG 2 cut(s) 201, 918
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 2 cut(s) 504, 585
BstC8I GCNNGC 2 cut(s) 183, 600
BstDEI CTNAG 3 cut(s) 237, 807, 874
BstDSI CCRYGG 1 cut(s) 832
BstEII GGTNACC 1 cut(s) 857
BstKTI GATC 1 cut(s) 910
BstMAI GTCTC 2 cut(s) 212, 263
BstMBI GATC 1 cut(s) 907
BstMWI GCNNNNNNNGC 1 cut(s) 775
BstNI CCWGG 2 cut(s) 201, 918
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 3 cut(s) 107, 199, 916
BstV1I GCAGC 2 cut(s) 241, 556
BstV2I GAAGAC 2 cut(s) 57, 286
BsuRI GGCC 2 cut(s) 533, 553
BtgI CCRYGG 1 cut(s) 832
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 2 cut(s) 183, 600
Cfr10I RCCGGY 1 cut(s) 517
Cfr13I GGNCC 4 cut(s) 44, 105, 552, 920
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 199
Csp6I GTAC 2 cut(s) 197, 243
CviAII CATG 4 cut(s) 143, 402, 643, 902
CviQI GTAC 2 cut(s) 197, 243
DdeI CTNAG 3 cut(s) 237, 807, 874
DpnI GATC 1 cut(s) 909
DpnII GATC 1 cut(s) 907
DraI TTTAAA 1 cut(s) 337
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 2 cut(s) 504, 585
EarI CTCTTC 2 cut(s) 504, 585
Eco47I GGWCC 3 cut(s) 44, 105, 920
Eco57I CTGAAG 2 cut(s) 321, 672
Eco91I GGTNACC 1 cut(s) 857
EcoO65I GGTNACC 1 cut(s) 857
EcoRI GAATTC 3 cut(s) 7, 138, 785
EcoRII CCWGG 2 cut(s) 199, 916
FaeI CATG 4 cut(s) 146, 405, 646, 905
FatI CATG 4 cut(s) 142, 401, 642, 901
FauI CCCGC 1 cut(s) 409
FauNDI CATATG 1 cut(s) 684
Fnu4HI GCNGC 3 cut(s) 255, 515, 570
Fsp4HI GCNGC 3 cut(s) 255, 515, 570
GluI GCNGC 3 cut(s) 255, 515, 570
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 2 cut(s) 533, 553
HapII CCGG 5 cut(s) 42, 108, 518, 530, 855
HgaI GACGC 1 cut(s) 65
Hin1II CATG 4 cut(s) 146, 405, 646, 905
HindIII AAGCTT 2 cut(s) 331, 600
HinfI GANTC 6 cut(s) 92, 219, 704, 828, 842, 913
HpaII CCGG 5 cut(s) 42, 108, 518, 530, 855
Hpy166II GTNNAC 2 cut(s) 243, 462
Hpy188I TCNGA 6 cut(s) 13, 607, 841, 877, 895, 912
Hpy188III TCNNGA 6 cut(s) 47, 89, 559, 617, 701, 773
Hpy8I GTNNAC 2 cut(s) 243, 462
Hpy99I CGWCG 3 cut(s) 59, 138, 457
HpyAV CCTTC 1 cut(s) 667
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 887
HpyCH4V TGCA 6 cut(s) 35, 66, 146, 170, 257, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 775
HpyF3I CTNAG 3 cut(s) 237, 807, 874
HpySE526I ACGT 1 cut(s) 887
Hsp92II CATG 4 cut(s) 146, 405, 646, 905
Kzo9I GATC 1 cut(s) 907
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 2 cut(s) 842, 857
Lsp1109I GCAGC 2 cut(s) 241, 556
LweI GCATC 1 cut(s) 382
MabI ACCWGGT 1 cut(s) 199
MaeII ACGT 1 cut(s) 887
MaeIII GTNAC 3 cut(s) 262, 857, 868
MalI GATC 1 cut(s) 909
MboI GATC 1 cut(s) 907
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 6 cut(s) 7, 138, 158, 408, 445, 785
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 6 cut(s) 20, 570, 586, 780, 856, 883
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 1 cut(s) 764
MspI CCGG 5 cut(s) 42, 108, 518, 530, 855
MspR9I CCNGG 3 cut(s) 109, 201, 918
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 2 cut(s) 201, 918
MwoI GCNNNNNNNGC 1 cut(s) 775
NciI CCSGG 1 cut(s) 109
NdeI CATATG 1 cut(s) 684
NdeII GATC 1 cut(s) 907
NlaIII CATG 4 cut(s) 146, 405, 646, 905
NlaIV GGNNCC 3 cut(s) 106, 421, 838
PciSI GCTCTTC 1 cut(s) 504
PctI GAATGC 1 cut(s) 35
PfeI GAWTC 5 cut(s) 92, 219, 828, 842, 913
PkrI GCNGC 3 cut(s) 256, 516, 571
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
PsiI TTATAA 1 cut(s) 816
Psp6I CCWGG 2 cut(s) 199, 916
PspEI GGTNACC 1 cut(s) 857
PspGI CCWGG 2 cut(s) 199, 916
PspN4I GGNNCC 3 cut(s) 106, 421, 838
PspPI GGNCC 4 cut(s) 44, 105, 552, 920
RsaI GTAC 2 cut(s) 198, 244
RsaNI GTAC 2 cut(s) 197, 243
RseI CAYNNNNRTG 1 cut(s) 764
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 3 cut(s) 255, 515, 570
Sau3AI GATC 1 cut(s) 907
Sau96I GGNCC 4 cut(s) 44, 105, 552, 920
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 3 cut(s) 109, 201, 918
SduI GDGCHC 1 cut(s) 217
SexAI ACCWGGT 1 cut(s) 199
SfaNI GCATC 1 cut(s) 382
SinI GGWCC 3 cut(s) 44, 105, 920
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 2 cut(s) 615, 847
SmoI CTYRAG 2 cut(s) 615, 847
Sse9I AATT 6 cut(s) 7, 138, 158, 408, 445, 785
SsiI CCGC 4 cut(s) 291, 416, 515, 708
StyD4I CCNGG 3 cut(s) 107, 199, 916
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 890
TaqI TCGA 3 cut(s) 217, 792, 882
TasI AATT 6 cut(s) 7, 138, 158, 408, 445, 785
TatI WGTACW 1 cut(s) 242
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 5 cut(s) 92, 219, 828, 842, 913
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 245
TseI GCWGC 2 cut(s) 254, 569
TspDTI ATGAA 3 cut(s) 131, 438, 522
TspGWI ACGGA 3 cut(s) 200, 298, 821
TspRI CASTG 1 cut(s) 245
VpaK11BI GGWCC 3 cut(s) 44, 105, 920
XapI RAATTY 4 cut(s) 7, 138, 408, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.