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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AsuC2I | CCSGG | 1 cut(s) | 91 |
| BcnI | CCSGG | 1 cut(s) | 91 |
| Bme1390I | CCNGG | 1 cut(s) | 91 |
| BmrFI | CCNGG | 1 cut(s) | 91 |
| BpuMI | CCSGG | 1 cut(s) | 91 |
| BseGI | GGATG | 1 cut(s) | 10 |
| BsiSI | CCGG | 1 cut(s) | 91 |
| BstF5I | GGATG | 1 cut(s) | 10 |
| BstSCI | CCNGG | 1 cut(s) | 89 |
| BtsCI | GGATG | 1 cut(s) | 10 |
| FaiI | YATR | 5 cut(s) | 17, 44, 86, 117, 156 |
| FokI | GGATG | 1 cut(s) | 17 |
| FspEI | CC | 9 cut(s) | 7, 17, 52, 61, 70, 76, 103, 104, 135 |
| HapII | CCGG | 1 cut(s) | 91 |
| HpaII | CCGG | 1 cut(s) | 91 |
| HpyCH4V | TGCA | 1 cut(s) | 19 |
| LpnPI | CCDG | 1 cut(s) | 104 |
| MaeIII | GTNAC | 1 cut(s) | 50 |
| MboII | GAAGA | 2 cut(s) | 100, 152 |
| MluCI | AATT | 2 cut(s) | 28, 81 |
| MnlI | CCTC | 1 cut(s) | 69 |
| MseI | TTAA | 1 cut(s) | 163 |
| MslI | CAYNNNNRTG | 1 cut(s) | 44 |
| MspI | CCGG | 1 cut(s) | 91 |
| MspR9I | CCNGG | 1 cut(s) | 91 |
| NciI | CCSGG | 1 cut(s) | 91 |
| RseI | CAYNNNNRTG | 1 cut(s) | 44 |
| SaqAI | TTAA | 1 cut(s) | 163 |
| ScrFI | CCNGG | 1 cut(s) | 91 |
| SgeI | CNNG | 2 cut(s) | 102, 103 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 44 |
| Sse9I | AATT | 2 cut(s) | 28, 81 |
| StyD4I | CCNGG | 1 cut(s) | 89 |
| TaqI | TCGA | 1 cut(s) | 111 |
| TasI | AATT | 2 cut(s) | 28, 81 |
| Tru1I | TTAA | 1 cut(s) | 163 |
| Tru9I | TTAA | 1 cut(s) | 163 |
| TspDTI | ATGAA | 1 cut(s) | 153 |