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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 57, 177 |
| AgsI | TTSAA | 1 cut(s) | 203 |
| ApoI | RAATTY | 2 cut(s) | 57, 177 |
| AspLEI | GCGC | 1 cut(s) | 29 |
| AsuHPI | GGTGA | 1 cut(s) | 53 |
| BccI | CCATC | 1 cut(s) | 131 |
| BfaI | CTAG | 1 cut(s) | 15 |
| BfoI | RGCGCY | 1 cut(s) | 30 |
| Bse3DI | GCAATG | 2 cut(s) | 57, 129 |
| BseMI | GCAATG | 2 cut(s) | 57, 129 |
| BsrDI | GCAATG | 2 cut(s) | 57, 129 |
| BstC8I | GCNNGC | 1 cut(s) | 11 |
| BstH2I | RGCGCY | 1 cut(s) | 30 |
| BstHHI | GCGC | 1 cut(s) | 29 |
| BstNSI | RCATGY | 2 cut(s) | 13, 110 |
| Cac8I | GCNNGC | 1 cut(s) | 11 |
| CfoI | GCGC | 1 cut(s) | 29 |
| CviAII | CATG | 2 cut(s) | 10, 107 |
| FaeI | CATG | 2 cut(s) | 13, 110 |
| FaiI | YATR | 3 cut(s) | 11, 32, 108 |
| FatI | CATG | 2 cut(s) | 9, 106 |
| FspBI | CTAG | 1 cut(s) | 15 |
| GlaI | GCGC | 1 cut(s) | 28 |
| HaeII | RGCGCY | 1 cut(s) | 30 |
| HhaI | GCGC | 1 cut(s) | 29 |
| Hin1II | CATG | 2 cut(s) | 13, 110 |
| Hin6I | GCGC | 1 cut(s) | 27 |
| HinP1I | GCGC | 1 cut(s) | 27 |
| HphI | GGTGA | 1 cut(s) | 53 |
| HpyAV | CCTTC | 2 cut(s) | 15, 155 |
| HpyCH4V | TGCA | 1 cut(s) | 103 |
| Hsp92II | CATG | 2 cut(s) | 13, 110 |
| HspAI | GCGC | 1 cut(s) | 27 |
| LmnI | GCTCC | 1 cut(s) | 24 |
| LpnPI | CCDG | 1 cut(s) | 149 |
| MaeI | CTAG | 1 cut(s) | 15 |
| MaeIII | GTNAC | 2 cut(s) | 41, 73 |
| MboII | GAAGA | 1 cut(s) | 140 |
| MluCI | AATT | 3 cut(s) | 36, 57, 177 |
| MseI | TTAA | 1 cut(s) | 96 |
| NlaIII | CATG | 2 cut(s) | 13, 110 |
| NmuCI | GTSAC | 1 cut(s) | 41 |
| NspI | RCATGY | 2 cut(s) | 13, 110 |
| PaeI | GCATGC | 1 cut(s) | 13 |
| SaqAI | TTAA | 1 cut(s) | 96 |
| SgeI | CNNG | 8 cut(s) | 22, 27, 76, 81, 101, 119, 123, 148 |
| SphI | GCATGC | 1 cut(s) | 13 |
| Sse9I | AATT | 3 cut(s) | 36, 57, 177 |
| SspMI | CTAG | 1 cut(s) | 15 |
| TasI | AATT | 3 cut(s) | 36, 57, 177 |
| Tru1I | TTAA | 1 cut(s) | 96 |
| Tru9I | TTAA | 1 cut(s) | 96 |
| TseFI | GTSAC | 1 cut(s) | 41 |
| Tsp45I | GTSAC | 1 cut(s) | 41 |
| XapI | RAATTY | 2 cut(s) | 57, 177 |
| XceI | RCATGY | 2 cut(s) | 13, 110 |
| XspI | CTAG | 1 cut(s) | 15 |